-RR MC-LR MC-RR MC-YR, China Environmental Science , )

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1 27,27(3)382~386 China Environmental Science -RR 1,2, 1,2, 1, 1 (1., 3722., 19).1,1.,1.µg/mL-RR(MC-RR)BY-2,,(ACP).,2d,.MC-RR,2d5.57%;MC-RR.d,;7d.3,,7.98%76.7%8.%.3ACP. -RRBY-2 X53.23 A 16923(27)33825 Effect of microcystin-rr on cell viability and nutrient physiology of tobacco BY-2 suspension cells. HUANG Wen-min 1,2, XING Wei 1,2, LI Dun-hai 1, LIU Yong-ding 1* (1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 372, China2.Graduate University of Chinese Academy of Sciences, Beijing 19, China). China Environmental Science, 27,27(3)382~386 AbstractAdopting.1, 1., 1.µg/mL microcystin-rr (MC-RR) to treat tobacco BY-2 suspension cells, the cell viability, contents of protein, soluble sugar, nitrate-nitrogen, and total phosphorus in cells were determineded; and the viability change condition of acid phosphatase (ACP) was detected. The cell viability and protein content after the cells were treated by middle and high concentration toxin for 2d decreased markedly compared with the contrast. The soluble sugar content decreased by treating of high concentration MC-RR, after exposure of 2d, it was only 5.57% of the contrast. The soluble sugar content increased in the later period of low concentration MC-RR treating. After the cells were treated by high concentration toxin for d, the content of nitrate-nitrogen in cells were lower markedly than the contrast, after the cells were treated by middle and low concentration toxin for 7d, the content of nitrate-nitrogen in cells were lowered. 3 groups of toxin treating all lowered the total phosphorus content. At the end of test, the phosphorus contently of low, middle, high concentration treating groups were 7.98%, 76.7% and 8.% of the contrast respectively. The ACP activity of 3 treating groups compared with the contrast appeared the tendency of first lowering then raising. Key wordsmicrocystin-rrtobacco BY-2 suspension cellcell viabilitynutrient physiology.,(mc),.mc6, MC-LRMC-RRMC-YR, MCMC-RR [1]., [2], [3].,MC [] [5], [6].MC. BY-2,,,,, (22CB123);863 (25AA611) *,, liuyd@ihb.ac.cn

2 3 -RR 383. MC-RR BY-2, [1,78],MC-RRBY-2, ;, MC-RR.,MC-RR,,MC (Nicotiana. tabacum L. cv. Bright Yellow 2).MS[MS,.5mg/L,1.3mg/L B1,2mg/L KH 2 PO 3%(W/V),.2mg/L 2,-D.1mg/L],pH r/min, (25±1),2h. :,5%,3h, 8r/min3min,.2,3,C 18,9%,,HPLC. Sigma,. 1.2 (GL-1LM, );(RV 6-ML 1-B,IKA,);(Waters 6, waters, ); (SANYO-MSE SONIPREP 15,MSE,); TECAN(SUNRISE,);Millipore (Millipore,). 1.3 MC-RR,,.1,1.,1.µg/mL. KCMS, MC-RR,.1,1.,1.µg/mL,,3.2,, 5,7,9d,, (ACP). 1. TTC [9],g 85nmOD(A 85 ); Bradford [1], (BSA); Kochert [11] ; [12] ; ;ACP. 1.5 ±, SPSSOne-Way ANOVA MC-RR 1, 2d,,,.,6d,,.TTC, [13].,,.

3 38 27 A85(g -1 ) 1 MC-RR Fig.1 Effect of MC-RR on the viability of BY-2 cells,* P<.5, 2.2 MC-RR,MC [1].2, 2d, 57.89%53.59%,..,;,.,.,. (mg/ml ) (d). 2 MC-RR Fig (d) Effect of MC-RR on the content of protein 2.3 MC-RR,.3,,,.~2d,,,.,,, 5d,,.MC-RR 2d, 5.57%,2d,.,,. 7d,9d 9.62%., MC,,,,. (mg/mg ) (d) 3 MC-RR Fig.3 Effect of MC-RR on the content of soluble sugar 2. MC-RR,., *

4 3 -RR 385.,d,71.86%. 7d ().,MC-RR.,,,,. MC-RR Fig. Effect of MC-RR on the content of nitrate-n in the BY-2 cells 2.5 MC-RR,.,. (mg/mg ) (mg/mg ) (d) MC-RR Fig.5 Effect f MC-RR on the content of phosphorus 5,d, ;5d, ; 7d,; (d), 7.98%76.7%8.%.MC-RR. 2.6 MC-RRACP ACP [15].6, ~5d,ACP ;ACP, 7d,ACP,9d, ACP 73.82%53.19%68.25%.ACP,.ACP [15].,MC5d,,ACP,7d,,ACP.ACP,MC,. ACP (U/mg ) MC-RRACP Fig.6 Effect of MC-RR on the activity of ACP in the BY-2 cells (d) 3.1 1,MC-RR2d,,d MC-RR, MC-RR..

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