Preparation of Novel Structured Lipids

Transcription

1 JOURNAL OF OLEO SCIENCE Copyright 2005 by Japan Oil Chemists Society JOS Setsuko HARA, Ayato KANDA and Yoichiro TOTANI Faculty of Engineering, Seikei University 3-1 Kichijoji-kitamachi 3, Musashino-shi, Tokyo , JAPAN Edited by K. Miyashita, Hokkaido Univ., and accepted April 18, 2005 (received for review March 7, 2005) Abstract: Structured lipids with n-6/n-3 polyunsaturated fatty acids PUFA or medium chain fatty acids MC at optimal ratios were prepared by lipase catalyzed transacylation of tuna orbital oil consisting of about 40 n-3 PUFA such as eicosapentaenoic acid EPA and docosahexaenoic acid DHA with purified linoleic acid or MC, such as caprylic, capric, and lauric acids, at 60. 1, 3- Specific immobilized lipases of Lipozyme TL-IM and Lipozyme RM- IM originated from Thermomyces lanuginosus and Rhizomucor miehei were used in the preparation, respectively. By controlling enzyme amount from IUN for Lipozyme TL-IM and 1-12 BAUN for Lipozyme RM-IM with reaction time from 4 to 24h, structured lipids at n-6/n-3 ratios from 1.3 till 6.9 were easily prepared by the reaction of tuna orbital oil with highly purified linoleic acid. MC was introduced into tuna orbital oil at various MC/n-3 PUFA ratios such as for caprylic, for capric, and for lauric acids, respectively during transacylation for 32 h. Lipozyme RM-IM was clearly shown a better enzyme for the present transacylation, compared to Lipozyme TL-IM in consideration of the above ratio values and triglycerides recovery. Key words: transacylation, Lipozyme TL-IM, Lipozyme RM-IM, n-3 PUFA, n-6 PUFA, MC Correspondence to: Setsuko HARA, Faculty of Engineering, Seikei University, 3-1 Kichijoji-kitamachi 3, Musashino-shi, Tokyo , JAPAN shara@ch.seikei.ac.jp Journal of Oleo Science ISSN print / ISSN online 473

2 S. Hara, A. Kanda and Y. Totani b Riozopus delemar Candida rugosa Fig Fig. 1 Preparation of Structured Lipids. 474

3 Table Thermomyces lanuginosus 75 Rhizomucor miehei m Table Table 1 Fatty Acid Composition of Substrates. Tuna Oil Linoleic Acid Caprylic Acid Capric Acid Lauric Acid Caprylic Acid 99.5 Capric Acid 99.3 Lauric Acid 99.1 Linoleic Acid (n-6 PUFA) EPA + DHA (n-3 PUFA) 39.4 Others

4 S. Hara, A. Kanda and Y. Totani Fig Fig Table Table Table Fig. 2 Procedure of Transacylation. Fig. 3 Effects of Enzyme Amounts on PUFA-TG Preparation Catalyzed by Lipozyme TL-IM or Lipozyme RM-IM. 476

5 Table 2 Comparison of n-6/n-3 Ratio of PUFA-TG Prepared. Lipozyme TL-IM Lipozyme RM-IM Effect of Enzyme Amounts Enzyme Amounts (Unit) 20 IUN 40 IUN 60 IUN 80 IUN 1 BAUN 4 BAUN 8 BAUN 12 BAUN Reaction Time (h) 8 8 PUFA Content (%) n-6/n-3 Ratio Enzyme Amounts 80 IUN 8 BAUN Effect of Reaction Time Reaction Time (h) 4 h 8 h 16 h 24 h 4 h 8 h 16 h 24 h PUFA Content (%) n-6/n-3 Ratio IUN: Interesterification Unit, BAUN: Batch Acidolysis Unit Fig. 4 6 Fig Fig Fig Fig. 4 Fatty Acid Composition of n-3 PUFA-C8-TG Prepared Fig Fig. 5 Fatty Acid Composition of n-3pufa-c10-tg Prepared. 477

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