Cornerstone Biochemical Reagents

Transcription

1 Cornerstone Biochemical Reagents Don t just rely on good fortune, use high quality products that you can trust Peace of Mind EMD Millipore is a division of Merck KGaA, Darmstadt, Germany.

2 Common applications for our high quality reagents include, but are not limited to: Western Blotting Transfection Bacterial Transformation Protein Extraction Protein Expression Cell Culture Cell Lysis Protein Purification Protein Concentration Dialysis/Buffer Exchange Achieve Your Research Goals with Highly Cited Calbiochem Reagents. EMD Millipore has years of experience in delivering the highest quality Calbiochem and Novagen products that have been cited in over 100,000 peer-reviewed articles and have enabled scientists worldwide to rapidly achieve their research goals. Our antibiotics, buffers, detergents, and other research essentials undergo extensive quality testing to ensure consistent performance and to provide you with a strong foundation in every experiment. For greater peace of mind, you can always count on the best in class technical support. 2

3 Antibiotics and Selection Agents Antibiotics are natural substances secreted by microorganisms that are toxic to other microorganisms, but are generally nontoxic toward higher organisms. The modern age of antibiotics began in the late 1920s, when Alexander Fleming discovered that the mold Penicillium notatum was able to block bacterial growth. Natural antibiotics had inadvertently been used for centuries, but with Fleming s discovery, the full potential of antibiotics could be appreciated. The key advantage of some antibiotics is their ability to selectively target a microorganism s metabolic pathway without seriously affecting the eukaryotic host. Because many metabolic activities of the bacterial cell differ from those in the mammalian cell, these differences in antibiotics can be exploited to develop new agents. Furthermore, antibiotic resistance has proven to be advantageous in a variety of research applications. Calbiochem antibiotics are manufactured in a controlled environment and perform with exceptional consistency to ensure the best results, lot-after-lot. Four primary methods by which antibiotics act on bacteria are: Inhibition of cell wall synthesis Inhibition of protein synthesis Inhibition of nucleic acid synthesis Anti-metabolic activity or competitive antagonism Antibiotics can also be categorized based on: Narrow- or Broad-spectrum Gram-positive or Gram-negative Bactericidal or Bacteriostatic Below are examples of antibiotics offered with corresponding number of citations: Product Description Cat No Available Sizes # Citations from 2000 to 2009 G 418 Sulfate, Cell Culture Tested mg,,, 2, 6200, 250 g,, Bafilomycin A1, Streptomyces griseus µg 460 Hygromycin B, Streptomyces sp., Cell Culture-Tested ml, 5 ml, 20 ml, 50 ml 2550 See page 6 for recent citations 3

4 Buffers Buffers are aqueous systems that resist changes in ph when small amounts of acid or base are added. Buffer solutions are composed of a weak acid (the proton donor) and its conjugate base (the proton acceptor). Buffering results from two reversible reaction equilibria in a solution wherein the concentration of proton donor and its conjugate proton acceptor are equal. Buffers have both intensive and extensive properties. The intensive property is a function of the pka value of the buffer acid or base. Most simple buffers work effectively in the ph scale of pka +/-1.0. The extensive property of the buffers is also known as the buffering capacity. It is a measure of the protection a buffer offers against changes in ph. Buffering capacity generally depends on the concentration of buffer solution. Buffers with higher concentrations offer greater buffering capacity. EMD Chemicals offers a wide selection of buffers, each tailored for specific applications. For greater flexibility some buffers can be purchased either as solids or as ready-to-use solutions. Below are examples of antibiotics offered with corresponding number of citations: Product Description Cat No Available Sizes # Citations from 2000 to 2009 Triethylammonium Acetate, 1 M PBS Tablets ea 1040 HEPES, Free Acid, ULTROL ,,,, 2550 See page 6 for recent citations 4

5 Detergents In order to understand the function and structure of membrane proteins, it is necessary to carefully isolate these proteins in their native and highly purified state. Although membrane protein solubilization can be accomplished by using amphiphilic detergents, preservation of their biological and functional activities can be a challenging process as many membrane proteins are susceptible to denaturation during the isolation process. Detergents solubilize membrane proteins by mimicking the lipid bi-layer environment. Type of Detergents: Main Features Type of Detergent Ionic Detergents Non-Ionic Detergents Zwitterionic Detergents Examples Anionic: Sodium dodecyl sulfate (SDS) Cationic: Cetyl methyl ammonium bromide (CTAB) TRITON -X-100, n-octyl-ß- D-glucopyranoside CHAPS, ZWITTERGENT Detergents Comments Contain head group with a net charge. Either anionic (- charged) or cationic (+ charged). Micelle size is determined by the combined effect of hydrophobic attraction of the side chain and the repulsive force of the ionic head group. Neutralizing the charge on the head group with increasing counter ions can increase micellar size. Useful for dissociating protein-protein interactions. The CMC of an ionic detergent is reduced by increasing the ionic strength of the medium, but is relatively unaffected by changes in temperature. Uncharged hydrophilic head group. Better suited for breaking lipid-lipid and lipid-protein interactions. Considered to be non-denaturants. Salts have minimal effect on micellar size. Solubilize membrane proteins in a gentler manner, allowing the solubilized proteins to retain native subunit structure, enzymatic activity and/or non-enzymatic function. The CMC of a non-ionic detergent is relatively unaffected by increasing ionic strength, but increases substantially with rising temperature. Offer combined properties of ionic and non-ionic detergents. Lack conductivity and electrophoretic mobility. Do not bind to ion-exchange resins. Suited for breaking protein-protein interactions. Below are examples of antibiotics offered with corresponding number of citations: Product Description Cat No Available Sizes # Citations from 2000 to 2009 ZWITTERGENT 3-14 Detergent , 2, 182 Digitonin, High Purity mg,, 470 CHAPS ,, 10 g, See page 6 for recent citations 5

6 Recent Citations Antibiotics and Selection Agents Hui, L., Yao, Y., et al. (2009) Inhibition of Janus kinase 2 and signal transduction and activator of transcription 3 protect against cecal ligation and puncture-induced multiple organ damage and mortality. J Trauma. 66(3): Petschnigg, J., Wolinski, H., et al. (2009) Good Fat, Essential Cellular Requirements for Triacylglycerol Synthesis to Maintain Membrane Homeostasis in Yeast. J. Biol. Chem. 284: Nijnik, A., Pistolic, J., et al. (2009) Human Cathelicidin Peptide LL-37 Modulates the Effects of IFN- on APCs. J. Immunol. 183: Detergents Schug, Z.T., Joseph, S.K. (2006) The Role of the S4-S5 Linker and C-terminal Tail in Inositol 1,4,5-Trisphosphate Receptor Function. J. Biol. Chem., 281: Zakrzewska, M., Wiedlocha, A., et al. (2009) Increased Protein Stability of FGF1 Can Compensate for Its Reduced Affinity for Heparin. J. Biol. Chem., 284: Wang, A., Rud, J., et al. (2009) Phosphorylation of Nur77 by the MEK-ERK-RSK Cascade Induces Mitochondrial Translocation and Apoptosis in T Cells. J. Immunol., 183: Buffers Hawkins, J., Robbins, M.D., et al.j (2008) Pharmacologic Inhibition of Site 1 Protease Activity Inhibits Sterol Regulatory Element-Binding Protein Processing and Reduces Lipogenic Enzyme Gene Expression and Lipid Synthesis in Cultured Cells and Experimental Animals. J. Pharmacol. Exp. Ther. 326: Wilsher, N.E., Court, W.J., et al. (2007) The Phosphoinositide-Specific Phospholipase C Inhibitor U73122 (1-(6-((17ß-3-Methoxyestra- 1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole- 2,5-dione) Spontaneously Forms Conjugates with CommonComponents of Cell Culture Medium. Drug Metab. Dispos., 35: Nichols, R.A., Dengler, A.F., et al. (2007) A Constitutive, Transient Receptor Potential-like Ca 2+ Influx Pathway in Presynaptic Nerve Endings Independent of Voltage-gated Ca 2+ Channels and Na + /Ca 2+ Exchange. J. Biol. Chem., 282: Other Related Products Applications Families of Products Affinity Chromatography Agonists and Antagonists Bead based assays Amino Acids Cell Culture Chelating Agents Cell Expression Denaturants Cloning DNA, RNA Polymerases Cytokine analysis DNA Markers Dialysis Dyes and Stains DNA analysis and interaction Enzymes Electrophoresis Nucleases and Nucleic Acids ELISA Organics and Inorganics Glycoanalysis - Agarose Nucleic acid purification - Water PCR - Glycerol Protein Folding and Refolding - Formaldehyde Protein Expression and PCR Reagents Purification Phosphatases Protein-to-protein interactions Polysaccharides RNA analysis Proteases Sample Preparation and Reducing Agents clean-up RNA Markers Transfection Salts, Acids and Bases Western Blotting Sample Loading Buffers Solvents Substrates Sugars 6

7 Antibiotics and Selection Agents Antifungal Agents Amphotericin B, Streptomyces sp. 100 mg Cerulenin, Cephalosporium caerulens 5 mg Clotrimazole 100 mg Cycloheximide Cycloheximide, High Purity 100 mg In Cycloheximide 1 ml In Sinefungin 2 mg Ketoconazole 50 mg Nystatin, S. noursei, Sterile, TC Grade 50 mg Nystatin, S. noursei Pimaricin, S. chattanoogensis 100 mg Polyoxin D, S. cacaoi var. aso 1 mg Radicicol, Diheterospora chlamydosporia 500 µg Antimicrobial Agents Actinomycin D, 7-Amino- 1 mg Actinomycin D, Streptomyces sp. Each Ampicillin, Sodium, Sterile 10 ml Ampicillin, Sodium, Sterile, TC Grade 20 ml Ampicillin, Sodium Salt Anisomycin, S. griseolus 10 mg Bacitracin 250 KU Bafilomycin A1, S. griseus 10 µg Benzalkonium Chloride 250 ml Blasticidin S, HCl, S. griseochromogenes 25 mg Blasticidin S, Streptomyces sp., 10 ml BLEOCIN, S. verticillus, CCT 10 mg 100 mg 250 mg BLEOCIN, S. verticillus, Sterile 10 ml Bleomycin Sulfate, S. verticillus 10 mg Carbenicillin, Disodium Salt 250 mg Cefotaxime, Sodium Salt 2 g Chetomin, Chaetomium sp. 1 mg Chloramphenicol Peace of Mind 1

8 Antibiotics and Selection Agents Antimicrobial Agents (continued) Chromomycin A3 10 mg Erythromycin, S. erythreus G 418 Sulfate, CCT 10 ml G 418 Sulfate, CCT 20 ml ml G 418 Sulfate, CCT 250 mg 500 mg Gentamycin Sulfate Gentamycin Sulfate, Sterile-Filtered 20 ml Hygromycin B in PBS, CCT 5 ml 20 ml 50 ml Hygromycin B, in 25 mm HEPES, CCT Hygromycin B, Streptomyces sp. Hygromycin B, Streptomyces sp., CCT Kanamycin Sulfate, S. kanamyceticus 5 ml 20 ml 100 KU 1 MU 5 MU 10 MU 100 mg 500 mg Kanamycin Sulfate, S. kanamyceticus 20 ml Kanamycin Sulfate, S. kanamyceticus, CCT Mitomycin C, S. caespitosus 10 mg Mitomycin C, S. caespitosus 2 mg Neomycin Sulfate Neomycin Sulfate, TC Grade 20 ml Oxytetracycline, Hydrochloride 10 g Paromomycin Sulfate Pen/Strept/Ampho B Soln. (100X) TC Grade 20 ml Penicillin G, Potassium Salt 100 MU Penicillin/Streptomycin, 100X, TC Grade 20 ml Polymyxin B Sulfate 500 mg Polymyxin B Sulfate, Sterile-Filtered 20 ml Puromycin, DiHCl 25 mg mg Puromycin, DiHCl, CCT Rifampicin 25 mg 100 mg Spectinomycin, Dihydrochloride, Pentahydrate 10 g Streptomycin Sulfate, Streptomyces sp Streptozotocin 250 mg Tetracycline, HCl Tetracycline, HCl, CCT 10 g 2 50 g 10 g 2 50 g Tobramycin, Free Base 100 mg Triclosan Tunicamycin, S. lysosuperficus 10 mg 50 mg Vancomycin, HCl, S. orientalis 250 mg Virstatin 25 mg Antiviral Agents KEY (MB Gr) Molecular Biology Grade, (CCT) Cell Culture Tested, (TG Gr) Tissue Culture Grade, (MU) Million Units, (TU) Thousand Units 3 -Azido-3 -deoxythymidine 10 mg Acyclovir 25 mg Glycyrrhizin, Ammonium Salt Penciclovir 100 mg Ribavirin 10 mg Lit. No. PB3704ENUS 2

9 Buffers Buffers BES, Free Acid, ULTROL Grade Bicine, ULTROL Grade BIS-Tris, ULTROL Grade CAPS, ULTROL Grade CHES, ULTROL Grade HEPES, Free Acid, MB Grade g HEPES, Free Acid, ULTROL Grade HEPES, Free Acid, ULTROL Grade, 1M HEPES, Sodium Salt, ULTROL Grade Imidazole, ULTROL Grade MES, Free Acid, ULTROL Grade MES, Sodium Salt, ULTROL Grade MOPS, Free Acid, MB Grade MOPS, Free Acid, ULTROL Grade MOPS, Sodium Salt, ULTROL Grade MOPS/EDTA Buffer, 10X Liquid Concentrate, MB Grade PBS Tablets 1 each PBS-TWEEN Tablets 1 each PIPES, Free Acid, ULTROL Grade PIPES, Sesquisodium Salt, ULTROL Grade PIPPS SSC Buffer, 20X Powder Pack, ULTROL Grade 2 pack SSPE Buffer Tablets 1 each TAE Buffer, 10X, MB Grade TAPS, ULTROL Grade TBE Buffer, 10X Powder Pack, ULTROL Grade 2 pack TES, Free Acid, ULTROL Grade TG-SDS Buffer, 10X Powder Pack, ULTROL Grade Tricine, ULTROL Grade pack Triethylammonium Acetate, 1 M Tris Base, MB Grade Tris Base, ULTROL Grade Tris Buffer, 1 M, ph 8.0, MB Grade Tris Buffer, 100 mm, ph 7.4, MB Grade Tris, HCl, MB Grade Tris, HCl, ULTROL Grade 250 g KEY (MB Gr) Molecular Biology Grade Peace of Mind Lit. No. PB3703ENUS

10 Detergents Zwitterionic Detergents ASB ZWITTERGENT Set 1 set ASB-14 ASB-14-4 ASB-16 ASB-C7BzO ASB-C8Ø CHAPS g CHAPSO DDMAB ZWITTERGENT 3-08 Detergent ZWITTERGENT 3-10 Detergent ZWITTERGENT 3-12 Detergent ZWITTERGENT 3-14 Detergent ZWITTERGENT 3-16 Detergent ZWITTERGENT Test Kit 1 kit Ionic Detergents Chenodeoxycholic Acid, Free Acid Chenodeoxycholic Acid, Sodium Salt Cholic Acid, Sodium Salt 50 g 250 g Cholic Acid, Sodium Salt, ULTROL Grade CTAB, MB Grade Deoxycholic Acid, Sodium Salt Deoxycholic Acid, Sodium, ULTROL Grade Glycocholic Acid, Sodium Salt Glycodeoxycholic Acid, Sodium Salt Lauroylsarcosine, Sodium Salt LPD-12 1 mg 2 mg SDS Tablets 1 ea SDS, 20% (w/v) 200 ml SDS, MB Grade 50 g Sodium n-dodecyl Sulfate Sodium n-dodecyl Sulfate, High Purity Taurocholic Acid, Sodium, ULTROL Grade Taurocholic Acid, Sodium Salt Taurodeoxycholic Acid, Sodium Salt Tauroursodeoxycholic Acid, Sodium Salt 2 50 g Peace of Mind 1

11 Detergents Non-ionic Detergents APO Detergent Set 1 set APO APO Big CHAP Big CHAP, Deoxy 250 mg BRIJ 35 Detergent, PROTEIN GRADE., 10% 50 ml BRIJ 35 Detergent, 30% Aqueous NDSB-195 NDSB g Cyclohexyl-n-hexyl-ß-D-maltoside, ULTROL Grade C12E9, PROTEIN GRADE Detergent, 10% 1 set C12E8, PROTEIN GRADE Detergent, 10% 1 set C12E Digitonin, High Purity 250 mg ELUGENT Detergent, 50% GENAPOL X-080, PROTEIN GRADE Det. 10% 50 ml GENAPOL X-100, PROTEIN GRADE Det. 10% 50 ml GENAPOL C-100, PROTEIN GRADE Det. 10% 50 ml HECAMEG MEGA-10, ULTROL Grade MEGA-9, ULTROL Grade n-octanoylsucrose n-octyl-ß-d-thioglucopyranoside, ULTROL Grade n-octyl-ß-d-glucopyranoside, ULTROL Grade 250 mg n-octyl-ß-d-glucopyranoside Non-detergent Sulfobetaines n-nonyl-ß-d-glucopyranoside n-hexyl-ß-d-glucopyranoside n-dodecanoylsucrose n-dodecyl-ß-d-maltoside, ULTROL Grade n-dodecyl-ß-d-glucopyranoside n-decanoylsucrose n-decyl-ß-d-maltopyranoside, ULTROL Grade NP-40 Alternative, PROTEIN GRADE 10% NP-40 Alternative NDSB NDSB ml 1000 ml n-octyl-ß-d-maltopyranoside PLURONIC F-127, PROTEIN GRADE 10% 50 ml Saponin TWEEN 80, PROTEIN GRADE Detergent, 50 ml % TWEEN 20, PROTEIN GRADE Detergent, 50 ml % TWEEN 20 Detergent 250 ml TWEEN 20 Detergent, MB Grade TRITON X-114, PROTEIN GRADE 10% 50 ml TRITON X-100 Detergent, MB Grade 50 ml TRITON X-100 Detergent, Hydrogenated 10 g TRITON X-100, Hydrogenated, PROTEIN 10 ml GRADE 10% TRITON X-100, PROTEIN GRADE 10% 50 ml TRITON X-100 Detergent 3 kg KEY (MB Gr) Molecular Biology Grade NDSB Set 1 set Lit. No. PB3702ENUS 2

12 OmniPur Amino Acids OmniPur Glycine OmniPur L-Histidine 2 50 kg EM-4810 EM-4840 EM-4860 EM-4870 EM-5450 Antibiotics OmniPur Amphotericin B Solubilized 100 mg EM-2500 EM-2501 OmniPur Ampicillin, Sodium Salt 2 EM-2200 OmniPur G418 Sulfate Antibiotic OmniPur Gentamicin Sulfate 2 EM-4700 EM-4705 EM-4710 EM-4730 OmniPur Kanamycin Sulfate 10 g EM-5880 OmniPur Tetracycline Hydrochloride EM-8990 OmniPur Penicillin G, Sodium Salt 20 ml EM-6400 OmniPur Penicillin/Streptomycin 20 ml EM-6402 OmniPur Streptomycin Sulfate EM-8610 OmniPur Actinomycin D 5 mg EM-2472 OmniPur Chloramphenicol EM-3130 OmniPur Hygromycin B, HPLC 100 mg EM-4260 OmniPur Hygromycin B 50Mg/ml 20 ml EM-4265 Buffers OmniPur 10X PBS Liquid Concentrate 4 l EM l EM-6504 OmniPur TRIS OmniPur 10X PBS Liquid Concentrate 2 4 l EM-9210 EM-9230 EM-9260 EM-6506 EM-6507 OmniPur 10X PBS, Premixed Powder EM-6508 OmniPur PBS Tablets 100 ea 200 ea EM-6500 EM-6501 OmniPur 20X SSC Buffer 4 l EM-8310 OmniPur 10X TAE Buffer OmniPur 25X TAE Buffer, Premixewd Powder 4 l 20 l 1 pc 1 pac EM-8720 EM-8725 EM-8750 EM-8770 OmniPur 5X TBE Buffer 4 l EM-8800 OmniPur 10X TBE Buffer 4 l 20 l 200 l 1 pac EM-8820 EM-8830 EM-8840 EM-8850 TE Buffer EM-8890 OmniPur 100X TE Buffer EM-8910 OmniPur HEPES OmniPur HEPES, Sodium Salt EM-5310 EM-5320 EM-5330 EM-5340 EM-5380 EM-5390 EM-5400 OmniPur Imidazole 50 g EM-5710 EM-5720 OmniPur BIS TRIS EM-2650 EM-2670 OmniPur 20X SSPE Buffer 4 l EM-8410 OmniPur MES 2 EM-6110 EM-6120 EM-6150 OmniPur MOPS 2 EM-6310 EM-6320 EM-6340 OmniPur 10X MOPS, Concentrate EM-6350 OmniPur TRIS Hydrochloride 2 EM-9310 EM-9350 EM-9380 OmniPur TRIS, 1.0M ph 7.5 EM-9285 OmniPur TRIS, 1.0M ph 8.0 EM-9290 OmniPur TRIS, 1.0M ph 9.0 EM-9295 OmniPur TRIS, 1.0M ph 10.0 EM-9299 OmniPur TCEP-HCl 2 g EM OmniPur 10X TG Buffer 4 l EM-9000 OmniPur TRICINE 2 EM-9010 EM-9050 OmniPur 10X TG-SDS Buffer 4 l EM-9015 OmniPur TBS 20X Liquid Concentrate 4 l EM-8320 OmniPur TBS 20X Ready Pack Powder 2 pac EM-8325 OmniPur 20X SSC Buffer, Premixed Powder 1 pac EM

13 OmniPur Buffers (continued) OmniPur 20X SSPE Buffer,Premixed Powder 1 pac EM-8450 OmniPur CHES EM-3100 OmniPur PIPES, Sodium Salt OmniPur Sodium Citrate, Dihydrate 250 g EM-6910 EM-6912 EM-7810 EM-7860 OmniPur BIS-TRIS Hydrochloride EM Carbohydrates OmniPur IPTG 2 EM-5800 EM-5810 EM-5815 EM-5820 OmniPur Sucrose Maltose Monohydrate D(+)-Glucose, Monohydrate OmniPur Dextran Sulfate Sodium OmniPur Dextran Sulfate, 50% Sterile OmniPur Ficoll kg 50 g EM-8510 EM-8550 EM-MX EM-MX EM-MX EM EM EM-3710 EM-3712 EM g EM-4550 EM-4555 OmniPur D-(-)-Ribose EM-7410 OmniPur Sucrose EM-8590 Sorbitol 500g EM-SX Chelating Agents OmniPur EDTA EM-4005 EM-4008 OmniPur EDTA, Disodium Salt, Dihydrate OmniPur EDTA, Disodium Salt, Dihydrate,, 5M 250 g EM-4010 EM-4050 EM-4055 OmniPur EGTA 50 g EM-4100 OmniPur 8-Hydroxyquinoline EM-4690 EM-4692 Denaturants OmniPur Guanidine Hydrochloride 10 kg EM-5010 EM-5020 EM-5030 OmniPur Urea OmniPur Guanidine Isothiocyanate g EM-9510 EM-9530 EM-9550 EM-5120 EM-5140 EM-5150 OmniPur Denaturation 10 l EM-3510 OmniPur Glyoxal, 40% EM-4910 Detergents OmniPur Sodium Dodecyl Sulfate, SDS EM-7910 EM-7960 OmniPur Sodium Dodecyl Sulfate 20% OmniPur Tween 20 (Polyoxyethylene (20) Monolaurate) OmniPur Tween 80 (Polyoxyethylene (20) Monooleate) OmniPur CHAPS OmniPur Triton X-100 Surfactant Dyes and Stains 200 ml 4 l 4 l 10 g 4 l 20 l EM-7990 EM-7991 EM-9480 EM-9485 EM-9490 EM-9495 EM-3050 EM-3055 EM-9400 EM-9410 EM-9440 EM-9460 OmniPur Coomassie Blue G EM-3300 OmniPur Coomassie Blue R g EM-3340 EM-3345 OmniPur 6X Loading Buffer 5ml EM-2100 OmniPur Bromocresol Green, Sodium Salt 2 EM-2800 OmniPur Bromophenol Blue, Sodium Salt 2 EM-2830 OmniPur Ethidium Bromide EM-4310 EM-4340 OmniPur Ethidium Bromide 10ml EM-4410 OmniPur Methylene Blue, Trihydrate 100g EM-6210 OmniPur Silver Pro Stain Kit kit EM-7490 OmniPur Xylene Cyanol FF 25g EM

14 OmniPur Organics and Inorganics OmniPur Agarose OmniPur Agarose PCR Plus OmniPur Agarose Super-Fine Resolution g EM-2120 EM-2125 EM-2005 EM-2010 EM-2020 EM-2081 EM-2082 OmniPur Agarose, Low Melting EM-2070 OmniPur Agarose, High Gel Strength EM-2090 OmniPur Ethyl Alcohol, Pure, 200 Proof 4 l EM-4450 EM-4455 OmniPur Glycerol OmniPur Acrylamide:Bis-acrylamide, 29:1, 40% OmniPur Acrylamide 4 l EM-4750 EM-4760 EM-1690 EM-1700 EM-1710 EM-1150 EM-1160 EM-1170 OmniPur Acrylamide, 40% W/V EM-1185 OmniPur Acrylamide: Bis-acrylamide, 19:1 Premixed Powder OmniPur Acrylamide: Bis-acrylamide, 19:1, 40% OmniPur Acrylamide:Bis-acrylamide,37.5:1, 40% OmniPur Acrylamide:Bis-acrylamide, 29:1 Premixed Powder OmniPur N,N -Methylene Bis Acrylamide OmniPur X-Gal 40 g 200 g EM-1190 EM-1200 EM-1290 EM-1300 EM-1310 EM-1490 EM g EM mg EM-2610 EM-2620 EM-9630 EM-9660 OmniPur 50X Denhardts 50ml EM-3610 OmniPur DEPC 2 EM-3660 OmniPur DNA Extraction Buffer 20 l EM-3700 OmniPur ACRIDINE ORANGE 50 g EM-1125 OmniPur ABTS Liquid Substrate EM-2006 OmniPur BCIP 500 mg EM-2510 OmniPur Blocking Agent in TBS EM-2552 OmniPur N,N -Methylene-Bis-Acrylamide 100ml EM % W/V OmniPur Bradford Method Protein Assay Kit EM-2740 OmniPur DNase I 50,000 Units EA 1 pc EM-3750 OmniPur Enviro-Coat EM-4190 EM-4200 OmniPur Fast Green FCF 50 g EM-4510 OmniPur Hybridization, 50% EM-5680 Formamide OmniPur Nonfat Powdered Milk 10 g EM-6250 OmniPur Neutralization 1l EM-6410 OmniPur Polyethylene Glycol 8,000 OmniPur Phenylmethyl Sulfonyl Fluoride 250 g EM-6510 EM-6520 EM-7110 EM-7130 OmniPur Polyvinylpyrrolidone (PVP) EM-7220 OmniPur Precipitation 20 l EM-7280 OmniPur RNase Inhibitor 2 KU EM-7480 OmniPur Sodium Lauroyl Sarcosine EM-8110 OmniPur TEMED 25 ml EM-8920 EM-8930 Ninhydrin 10 g EM-NX EM-NX EM-NX Proteins and Enzymes OmniPur BSA, Fraction V 2 EM-2905 EM-2910 EM-2930 EM-2960 EM-2980 OmniPur Lysozyme, Egg White OmniPur Horse Raddish Peroxidase Rz g 25000pc pc EM-5950 EM-5960 EM-5975 EM-4240 EM-4245 Pronase E EM Pancreatin EM-PX Pepsin Powder EM-PX Reducing Agents OmniPur 2-Mercaptoethanol EM ml EM-6050 OmniPur DTT (Dithiothreitol) 2 EM-3860 EM-3870 EM-3890 OmniPur DTE EM

15 OmniPur Salts, Acids and Bases OmniPur Sodium Chloride 2 EM-7710 EM-7760 EM-7780 Chloride Phosphate 197 g EM-PW OmniPur Magnesium Chloride, Hexahydrate EM-5980 EM-5982 OmniPur Magnesium Chloride, 1M EM-5985 OmniPur Calcium Chloride, Dihydrate OmniPur Cesium Chloride OmniPur Ammonium Acetate OmniPur Ammonium Sulfate OmniPur Ammonium Persulfate 2 2 EM-3000 EM-3004 EM-3030 EM-3040 EM-3045 EM-2145 EM-2149 EM-2150 EM-2160 EM-2170 EM-2300 EM-2310 OmniPur Ammonium Persulfate, Pouches 50 ea EM-2400 OmniPur Boric Acid 2 EM-2710 EM-2720 EM-2730 OmniPur Citric Acid, Anhydrous EM-3200 EM-3220 OmniPur Sodium Phosphate, Dibasic, Heptahydrate OmniPur Sodium Phosphate, Monobasic, Monohydrate OmniPur Potassium Chloride OmniPur Sodium Acetate, Anhydrous OmniPur Sodium Acetate, Trihydrate EM-8210 EM-8240 EM-8290 EM-8295 EM-7300 EM-7320 EM-7510 EM-7560 EM-7610 EM-7660 Maleic Acid 50 kg EM-MX OmniPur Lithium Chloride EM-5910 EM-5922 OmniPur Casamino Acids EM-2240 OmniPur Cacodylic Acid 50 g EM-2990 Oleic Acid EM-OX Solvents OmniPur Formamide 4 l EM-4610 EM-4650 EM-4670 OmniPur Phenol:Chloroform: Isoamyl Alcohol, 25:24:1 OmniPur Phenol, Saturated (TE Buffer Saturated) OmniPur Phenol, Double Distilled OmniPur Phenol Saturated, (Citrate Buffered ph 4.5) OmniPur Phenol:Chloroform: Isoamyl Alcohol, 125:24:1 (Citrate Buffered ph 4.5) OmniPur Phenol:Chloroform:Isoamyl Single Phase. 25:24:1 OmniPur Chloroform: Isoamyl Alcohol, 24:1 400 ml 400 ml 400 ml 400 ml EM-6805 EM-6810 EM-6705 EM-6710 EM-6610 EM-6650 EM-6700 EM-6702 EM-6800 EM-6802 EM ml EM-3155 EM-3160 OmniPur Dimethyl Sulfoxide EM-2951 OmniPur Chloroform 950 ml EM-3150 OmniPur Isopropyl Alcohol EM Water OmniPur Water, DEPC Treated, Sterile, Nuclease-Free Water, WFI Quality, Sterile Purified Water 5 l 10 l 5 l 10 l 20 l 200 l EM-9601 EM-9602 EM-9610 EM-9612 EM-9613 EM EM EM EM EM EM OmniPur A.C.E. Sequencing Water EM-2017 OmniPur Water, Sterile 5 l 10 l EM-9801 EM-9811 EM-9812 OmniPur Water, Sterile, Nuclease Free, Low Endotoxin EM-9820 Lit. No. PB3706ENUS 4

16 Western Blotting with OmniPur Quick Reference Product Guide OmniPur Acrylamide:Bis-acrylamide, 29:1, EM % Agarose C melt range, DNA Fragments EM-2070 >500kbp PCR plus Agarose C melt range, DNA EM-2120 Fragments >1000bp PCR plus Agarose C melt range DNA EM-2125 Fragments>1000bp OmniPur Ammonium Persulfate 2 EM-2300 OmniPur Ammonium Persulfate, Pouches 1 pack EM-2400 OmniPur Bromophenol Blue, Sodium Salt 2 EM-2830 OmniPur BSA, Fraction V EM-2905 OmniPur DTT EM-3860 OmniPur EDTA EM-4005 OmniPur EDTA, Disodium Salt, Dihydrate 250 g EM-4010 OmniPur Glycerol EM-4750 OmniPur Magnesium Chloride, Hexahydrate EM-5980 OmniPur PBS Tablets 1 pack EM-6500 OmniPur 10X PBS Liquid Concentrate 4 l EM-6505 OmniPur 10X PBS Liquid Concentrate, Sterile EM-6506 OmniPur 10X PBS, Premixed Powder 1 pack EM-6508 OmniPur Phenylmethyl Sulfonyl Fluoride7110 EM-7110 OmniPur Protein Molecular Weight Marker, EM-7331 Wide Range OmniPur Sodium Chloride EM-7710 OmniPur Sodium Dodecyl Sulfate EM-7910 OmniPur TEMED 25 ml EM-8920 OmniPur 10X TG Buffer, Liquid Concentrate 4 l EM-9000 OmniPur 10X TG-SDS Buffer, Liquid Concentrate 4 l EM-9015 OmniPur TRIS EM-9210 OmniPur TRIS Hydrochloride EM-9310 OmniPur Triton X-100 Surfactant EM-9410 OmniPur Tween 20 (Polyoxyethylene (20) EM-9480 Monolaurate) OmniPur Tween 80 (Polyoxyethylene (20) EM-9490 Monooleate) OmniPur Glycine 2 50 kg EM-4810 EM-4840 EM-4860 EM-4870 Peace of Mind 1

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18 The M mark is a trademark of Merck KGaA, Darmstadt, Germany. Lit No.PB3701ENUS Job No. LS SBU Millipore Corporation. All rights reserved M Lit. No

19 Western Blotting with OmniPur Prepare Western Blotting Buffers using OmniPur products Buffer Product Description VWR Cat #* Lysis Buffer 0.15M NaCl EM mM EDTA, ph 8 EM % Triton X-100 EM mM Tris HCL, ph 7.4 EM :1000 5M DTT EM : mM PMSF EM x Sample Buffer (2105) 130mM Tris HCL, ph 8.0 EM % (v/v) Glycerol EM % (w/v) SDS EM % Bromophenol blue EM % DTT EM x Resolving Gel Buffer: 100 ml 0.8g SDS (add last) EM g Tris base (=3M) EM x Stacking Gel Buffer: 100 ml 0.4g SDS (add last) EM g Tris base (=0.5M) EM-9210 Buffer Product Description VWR Cat #* 10x Running Buffer: 1L 10x Transfer Buffer: 1L Blocking Buffer: 0.5L Stripping Buffer: 0.5L 30.3g Tris base (=0.25M) EM g Glycine (=1.92M) 10g SDS (=1%, add last) (Cat. No. 9000, ready-to-use) EM-4810 EM-7910 EM g Tris base (=0.25M) EM g Glycine (=1.92M) EM % Bovine serum albumin EM-2905 Make up in PBS and sterile EM-6505 filter Then add 0.05% Tween 20 EM M Glycine, ph 2.5 EM % Tween 20 EM-9490 * Catalogue numbers offered are used to prepare solutions for buffers. They do not represent the ready-to-use molar solutions. Lit. No. PB3705ENUS 2