S T-2691 TAPS, Free Acid T-2695 TBE Buffer,10X concentrate powder T-2707 TES, FREE ACID T-1050 TES, High Purity T-2709 T-2711

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2 Protein Purification In order to characterize the structure, function and interaction of proteins with their environment it is essential to isolate the proteins of interest. Protein Purification is a process that involves steps associated with dissolution and isolation of proteins. For safe extraction of proteins, we carry products that form essential components of protein purification kits or used independently in research endeavors. Our protease inhibitor cocktails are a unique series of enzyme inhibitors that knock out specific proteases to avoid peptide bond hydrolysis and prevent protein destruction. Remove unwanted phosphatases by adding our phosphatase inhibitors which are also available in cocktail forms that selectively target particular contaminants. A complete package! Along with Protease and Phosphatase inhibitors we also specialize in buffers and detergents without which protein purification process is incomplete. Biological Buffers BES [N,N-bis(2-Hydroxyethyl)-2- B-2582 aminoethanesulfonic acid] B-2589 Bicine Ultra-Pure P-2815 Bis-Tris Propane C-1033 CAPS D-2577 Distilled water, Laboratory reagent grade H-1030 HEPPS M-1219 MES Buffer M-2697 MES Monohydrate M-2697 MES Monohydrate M-2699 MES ULTRAPURE M-2699 MES ULTRAPURE M-2721 MOPS O-2573 Orange-G Loading Dye 6X Solution P-2825 PBS 10x Powder with Ca and Mg P-2865 PIPES Sodium Salt P-2869 Potassium Acetate P-2871 Potassium Acetate ACS GRADE P-2885 Potassium Phosphate Dibasic P-2887 Potassium Phosphate, Dibasic, ACS Grade P-2891 Potassium Phosphate, Monobasic P-2893 Potassium Phosphate, Monobasic, ACS S-2857 Sodium Phosphate Dibasic Heptahydrate S-2859 Sodium Phosphate Dibasic, Anhydrous S-2867 Sodium Phosphate Monobasic Anhydrous S-2863 Sodium Phosphate Monobasic Monohydrate Sodium Phosphate Monobasic Monohydrate, S-2865 ACS Grade T-2691 TAPS, Free Acid T-2695 TBE Buffer,10X concentrate powder T-2707 TES, FREE ACID T-1050 TES, High Purity TG 10X Solution [TRIS-Glycine Buffer 10X T-2709 Solution] TGS 10X Solution [TRIS-Glycine-SDS Buffer 10X T-2711 Solution] T-2731 Transfer/Electro Blotting 10X Solution T-2733 TRICINE T-2735 Tricine-SDS Sample Buffer 2X Solution T-2745 TRIS Acetate ultra-pure T-2747 TRIS Buffered Saline, 10X solution T-2749 TRIS HCI 1M Stock Solution T-2749 Potassium Acetate ACS GRADE T-2751 TRIS-Acetate-EDTA [TAE], 10X solution T-2757 Triton Lysis Buffer 1

3 Partial list of Protease/Phosphatase/Turbocharged Inhibitor Cocktails Components Specificity of Inhibition P-1510 Protease Inhibitor Cocktail I AEBSF, Aprotinin, E-64, EDTA, Leupeptin Hemisulfate Serine, cysteine, and trypsin-like proteases; esterases; metalloproteases P-1511 Protease Inhibitor Cocktail II AEBSF, bestatin, E-64, EDTA, pepstatin A aminopeptidases; metalloproteases P-1542 Protease Inhibitor Cocktail III AEBSF, aprotinin (recombinant), bestatin, E-64, leupeptin, pepstatin A Serine, cysteine, aspartic, and trypsin-like proteases; aminopeptidases P-1513 Protease Inhibitor Cocktail IV AEBSF, E-64, Pepstatin A, Phenanthroline metalloproteases P-1514 Protease Inhibitor Cocktail V AEBSF, Aprotinin, E-64, Leupeptin Hemisulfate AEBSF, Aprotinin, E-64, Leupeptin Hemisulfate Serine, cysteine, and trypsin-like proteases P-1545 Protease Inhibitor Cocktail VI AEBSF, bestatin, E-64, Leupeptin Hemisulfate, phenanthroline, pepstatin A amiopeptidases; metalloproteases Trypsin-like Proteases P-1546 Protease Inhibitor Cocktail VII AEBSF, bestatin, E-64, pepstatin A, phosphoramidon, Leupeptin Hemisulfate metalloproteases P-1548 Protease Inhibitor Cocktail VIII ALLN, antipain, E-64 Calpain I/II, cathepsin A/B, cathepsin L/S, papain, cysteine proteases P-1517 Phosphatase Inhibitor Cocktail I (-)-p-bromotetramisole oxalate, cantharidin, microcystin LR Alkaline phosphatases, protein phosphatase 1 and 2A (PP-1/PP-2A) P-1518 Phosphatase Inhibitor Cocktail IISolid/Solution Imidazole, sodium (orthovanadate, fluoride, Tartrate Dihydrate, molybdate) Protein tyrosine phosphatase, acid phosphatases, alkaline phosphatases, Acid and Phosphoprotein Phosphatases P-1549 Phosphatase Inhibitor Cocktail III Sodium (orthovanadate, fluoride, pyrophosphate), β-glycerophosphate Ser/Thr phosphatases, acid phosphatases, alkaline phosphatases, PP-1/PP-2A P-1550 Phosphatase Inhibitor Cocktail IV (-)-p-bromotetramisole oxalate, cantharidin, Calyculin A, Discodermia calyx Alkaline phosphatases, protein phosphatases 1 and 2A (PP-1 and PP-2A) T-2492 Turbocharged Protease Inhibitor Cocktail AEBSF HCl, Aprotinin, Bestatin, Pepstatin, E-64. Cysteine, Aspartic and Trypsin-like Proteases, Metalloproteases, Papain, and Cathepsin A/B Turbocharged Phosphatase Inhibitor Cocktail Imidazole, Sodium orthovanadate, Sodium fluoride, Sodium tartrate dihydrate, Sodium molybdate, Sodium Pyrophosphate, Glyserophosphate, Bromotetramisole Oxalate Protein tyrosine phosphatase, Acid phosphatases, Alkaline phosphatases, PP-1/ PP-2A, Acid and phosphoprotein phosphatases, Ser/Thr Phosphatases T AG Scientific Inc. 2 agscientific.com

4 Individual Inhibitors Components Specificity of Inhibition A-1018 AEBSF A-1448 Antipain Dihydrochloride A-1420 Aprotinin B-1075 Bestatin HCl Serine proteases such as thrombin, plasmin, kallikrein, chymotrypsin, and trypsin. Also inhibits TGFβ (transforming growth factor beta), moderately inhibits CD26 (Dipeptidyl peptidase IV: DPP IV) Papain and trypsin, and to a smaller extent plasmin Kallikrein, trypsin, chymotrypsin, and various serine proteases Aminopeptidase and Leukotriene A4 hydrolase (LTA4H). Irreversible inhibitor, lower toxicity than PMSF or DFP More specific to papain and trypsin than leupeptin and 100-fold more potent than elastatinal. Competitive, reversible inhibitor, proteolytic inhibitor in radioimmunoassays of polypeptide hormones. Binds to cell surfaces. Also activates macrophages and T lymphocytes. C-1031 Calyculin A, Discodermia calyx protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A-C) Also potent non-phorbol tumor promoter P-1685 DPP IV E-2030 E-64 E-2044 Elastatinal L-1165 Leupeptin Hemisulfate M-1157 MG-132 P-2401 PMSF L-1165 Leupeptin Hemisulfate catalyzes the hydrolysis of N-terminal dipeptides from polypeptides with proline or alanine in the penultimate position. Cysteine proteases, Calpain, cathepsin K and cathepsin S phenylmethylsulfonyl fluoride (PMSF, sc- 3597) degradation of the H1 histone and high mobility group (HMG) proteins. Also inhibits Neutrophil Elastase. Serine and cysteine proteases. Often used for inhibition of plasmin, trypsin, papain, kallikrein, and cathepsin B. Blocks various apoptotic pathways in T cells. Proteasome inhibitor, reduces the degradation of ubiquitin-conjugated proteins in mammalian cells and permeable strains of yeast by the 26S complex without affecting its ATPase or isopeptidase activities. serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). Serine and cysteine proteases. Often used for inhibition of plasmin, trypsin, papain, kallikrein, and cathepsin B. Blocks various apoptotic pathways in T cells. Potent, orally active. Irreversible inhibitor Competitive inhibitor. Reversible inhibitor Specific, potent, reversible, and cell-permeable. Reversible inhibitor Reversible inhibitor 3

5 Biological Detergents Biological detergents are critical tools for the study of membrane proteins. They are vital for the isolation and purification of the proteins and are used in the primary solubilization step of reconstitution. Biological detergent solutions are also used in a variety of bioprocessing methods including electrophoresis, immunostaining, DNA extraction, and drug manufacture A high-quality biological detergent with the right properties can increase your yield and lower your costs. For your protein solubilization and protein purification needs, AG Scientific carries the highest quality zwitterionic CHAPS, Octyl-β-D-Glucopyranoside (OG), Non-Ionic DDM, Synthetic Zwitterionic Sulfobetaines, Protein Solubilizers, Triton X-100, Tween 80 and dust-free SDS to name a few. Ionic Biological Detergents Ionic Biological detergents have a hydrophilic head group that is charged either negatively (anionic) or positively (cationic). Ionic detergents are used for the denaturation of proteins and complete disruption of cellular structure. It is sensitively affected by ph, ionic strength, and the nature of the counter ion, and can interfere with downstream charge-based analytical methods. S-2066 Dust Free-SDS, Sodium Dodecyl Sulfate S-2428 SDS Solution (10% in H₂O) S-2451 Sodium Dodecyl Sulfate (SDS), powder form Non-Ionic Biological Detergents Non-ionic Biological detergents have a hydrophilic head group that is uncharged. They break protein-lipid, lipidlipid associations but not protein-protein interactions, and most of them do not denature proteins. That s why non-ionic biological detergents are considered as mild surfactants comparing to harsh surfactant, ionic detergents, to denature protein structure. By not separating protein-protein bonds, non-ionic detergents allow the protein to retain its native and active form. D-1304 n-dodecyl-beta-d-maltopyranoside (DDM) P-1504 Protein Solubilizer 35, Brij-35 N-2366 NP-40 (10% in H2O) P-1505 Protein Solubilizer 40, NP-40 N-2410 NP-40 Lysis High Salt Buffer Solution O-1036 n-octyl-β-d-glucopyranoside (OG) P-1508 Protein Solubilizer Set 1, Sterile 10% (Tween 20, Tween 80, Triton 80, Triton X-100, NP-40, & BR) P-1496 Protein Solubilizer X-114, Triton X-114 P-2387 PBS-Tween Tablets P-1498 Protein Solubilizer 80, Tween 80 T-2407 TBS/ Tween20 10X Solution P-1500 Protein Solubilizer X-100, Triton X-100 T-1056 Triton X Non-Ionic Detergent EIA P-1502 Protein Solubilizer 20, Tween 20 T-2779 Tween 20, 10% Solution AG Scientific Inc. 4 agscientific.com

6 Zwitterionic Biological detergents Zwitterionic Biological detergents have characteristics of both ionic and non-ionic detergent types. Its headgroups are hydrophilic and contain both positive and negative charges in equal numbers, resulting in zero net charge. Zwitterionic detergents are cause less denaturation of proteins than ionic detergents by protecting the native charge of protein molecules similar to non-ionic biological detergents but are more harsh surfactants. They are more efficient than non-ionic biological detergents at disrupting protein-protein bonds and reducing aggregation. These properties have been used for chromatography, mass spectrometry, and electrophoresis methods, and solubilization of integral membrane proteins. A-2187 ASB-14 C-1019 CHAPS, High Purity C-1461 CHAPSO S-1018 Sulfobetaine-8 S-1020 Sulfobetaine-10 S-1022 Sulfobetaine-12 S-1024 Sulfobetaine-14 S-1026 SulfoBetaine-16 Non-Detergent Sulfobetaines (NDSB) Non-detergent sulfobetaines (NDSB) consists sulfobetaine hydrophilic groups like those of zwitterionic detergents and short hydrophobic chains that cannot aggregate to form micelles. Therefore, NDSB s are not considered as detergent. They have been reported to improve the extraction yield of membrane, nuclear and cyto-skeletal associated proteins. NDSB s are used for protein folding, renaturation, and crystallization processes D-1089 NDSB-195 D-1119 NDSB-201 D-1091 NDSB-211 D-1121 NDSB-256 Corporate Promise AG Scientific, Inc. is an ISO 9001: 2008 certified facility since 2005; we will handle your projects with integrity and privacy. Comprehensive customer service and accountability at all phases of your project is our pledge. We value you as our customer and will work until your needs are met. We will listen to your requirements and be extremely responsive to your needs. On time delivery is a hallmark of our organization. You will be consistently informed of the status of your project so that you can confidently & effectively communicate to your organization. Our desire is to make this the best overall experience and earn a life-long client. This is our solemn commitment to you. 5

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