Latest Innovations in LC/MS/MS from Waters for Metabolism and Bioanalytical Applications

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1 Latest Innovations in LC/MS/MS from Waters for Metabolism and Bioanalytical Applications Ignatius J. Kass Senior Field Marketing Manager Pharmaceutical MS

2 Challenges in Pharmaceutical Sample Analysis Quantitative Bioanalysis Robust reliable methods Sensitivity Analyte confirmation Matrix effects Metabolites In Safety Testing Obtain qualitative data? Metabolic Profiling Confidence Sensitivity Throughput Obtain quantitative data?

3 New Additions to the Xevo Family Class leading UPLC MS and MS/MS performance Technology for simultaneous targeted quantification and high-sensitivity full scan MS and MS/MS monitoring The most sensitive, exact-mass, quantitative and qualitative benchtop MS/MS system Uses UPLC/MS E, a simple, patented method of data acquisition to comprehensively catalog complex samples in a single analysis

4 Analytical Workflow Engineered Simplicity Prepare IntelliStart technology for simplified set-up Analyze Enhanced compatibility with ACQUITY UPLC Performance and usability through Engineered Simplicity TM Automated calibration check and system monitoring Outstanding instrument sensitivity and selectivity Interpret MassLynx application managers, utilizing chemical intelligence, make sense of large, complex data sets Decide Arrive at business / research critical information faster

5 Intellistart IntelliStart Automated MS Resolution & Calibration Checks Performance and usability through Engineered Simplicity TM IntelliStart Automated MRM Method Developer Quanpedia Automated MRM Time Window & Data Rate Scheduling IntelliStart Automated LC/MS System Check Automatically ensure the system is ready to run IntelliStart Automated System Monitoring.

6 Innovations Providing Solutions for Quantitative Bioanalysis Radar Faster method development Qualitative profiling capability Step Wave Increased sensitivity Tools to assist with Regulatory Guidelines Matrix Factor determination MIST Guidelines

7 Method Development: More Depth to Your Data Fluticasone 501> Fluticasone 501> Radar Solvent Blank Radar Solvent Blank Radar Plasma Blank Radar Plasma Blank Phospholipids 184> Time0 Phospholipids 184> Time

8 Calculating a Spiked Simple MethodMatrix Factor XIC Analyte XIC Internal Standard Solvent + Analyte and Internal Standard Extracted Matrix + Analyte and Internal Standard A= 100 A= 90 A= 95 A= 85 MF = A matrix /A solvent MF = 90/100 =.90 MF = A matrix /A solvent MF = 85/95 =.89 IS Normalized MF = MF AN /MF IS IS Normalized MF =.90/.89 = 1.01

9 Alternate Approach for Calculating Matrix Factors LC Column MRM Signal 1) Determine AnalyteProfile AN and IS injected through the column 2) Inject Solvent Blank 3) Inject Extracted Matrix Blank Solvent Blank injected with post column infusion of AN and IS Extracted Blank injected with post column infusion of AN and IS AN and IS infused post-column Analyte = AN Internal Standard = IS

10 Automating Matrix Factor Calculations: Integrated Intellistart Fluidics

11 Matrix Factor Calculation Results Time Time

12 Quantitative Bioanalysis: Pushing the Sensitivity Barriers Potent, low dose compounds Biologics and Therapeutic peptides challenge the sensitivity requirements: Multiple charge states Many fragments Desmopressin

13 Desmopressin 25x S:N Xevo TQ-S Xevo TQ

14 StepWave, Radar and ScanWave Enabled Analysis: More Information Ibuprofen - MRM Glucuronide Metabolite Full Scan MS Hydroxyl - Glucuronide Metabolite - Full Scan MS Ketone Metabolite - Full Scan MS

15 ScanWave Enhanced Product Ion Spectra Ibuprofen MRM ScanWave Collision Cell Enhanced product ion spectra Ketone Metabolite Glucuronide Metabolite Hydroxyl Glucuronide Metabolite

16 An alternate Approach to Full Scan Profiling Metabolites Screen for selected panel of metabolites Commonly reported metabolites User definable Predicts metabolite MRMs based upon Parent Compound MSMS Generates MANY MRMs 1 msec dwell With RADAR, full scan MS may be added to the experiment

17 Develop Theoretical MRMs to Investigate MSMS of Parent Compound Identify Product Ions From Parent Compound Select Metabolites from list of probable transformations MS Source and Analyzer Conditions Automatic Product Ion Selection Spectra of Parent Automatically Obtained from MassLynx Calculate MRMs and Write MS Method File imrm Method List of Potential Metabolites from Text File

18 imrm method

19 Compare Full Scan vs MRM Experiment Full Scan MS MRM Group hr 17_4_005a _4_005a _4_005a 100 XIC Group hr 12: MS2 ES e : MS2 ES e5 XIC : MS2 ES e5 XIC Dealkylation Hydroxylation Demethylation 1.84 MRM > MRM > MRM > _4_005a : MS2 ES e6 XIC Parent 2.11 MRM 442.2> Time 0 Time

20 m/z 428 -Demethylation 1.84 MRM > PIC Scan Acquisition e Full Scan MS XIC m/z

21 Metabolic Stability Plots Demethylation Hydroxylation

22 Latest innovations in Metabolite Profiling Xevo G2 QTof Intellistart Maximizes instrument uptime and ensures optimal performance and consistency From Lab to Lab and user to user QuanTof MS E Quantitative information in addition to full high resolution scan accurate mass profiling Structural analysis for metabolite localization

23 Metabolite Identification WorkFlow For in-vitro/in-vivo samples Resolve metabolites chromatographically UPLC strategy for sensitivity and resolution Detect metabolites UPLC/MS, MS E Exact mass, Data rich information Eliminate background and control peaks Identify metabolites MetaboLynx for data processing Identification of expected and unexpected metabolites Mass defect filtering Confirm metabolite structures MassFragment for metabolite structure assignment and confirmation Quantify metabolites Absolute or relative quantification of metabolites

24 Alternate Scanning LC-MS (LC-MS E ) time resolved accurate mass measurements LE LE m1 sec EE D. EE Gas cell collision energy alternates between MS E low (typically, 5 ev) and is a UNBIASED process elevated energy (typically,5 ev linear ramp from 15 ev - 42 ev) LC-MS E alternate scanning provides quantitative accurate mass measurement for all detectable peptide precursor and product ions throughout UPLC separation EE EE

25 MetaboLynx XS: 1 µm Human Liver Microsome Verapamil Incubation Samples Found Metabolites Combined Metabolites MS Spectrum Metabolite XIC of Metabolite XIC of Metabolite in Control Parent Drug Structure

26 MSMS Information Displayed in the Fragment Analysis Window. Product Ion Information Precursor Ion Information Neutral Loss Information

27 Verapamil Product Ion Spectrum Interpretation Ver0319_ (8.845) : TOF MS ES+ 4.15e m/z

28 MassFragment Structural Assignment

29 Verapamil Fragment Assignment to Structure Ver0319_ (8.845) mDa 2: TOF MS ES+ 4.15e5-0.1 mda -0.1 mda -0.5mDa -0.1mDa -0.0 mda m/z

30 Samples, Time Course

31 Metabolic Stability Plot

32 Alprazolam Quantitation Curve QuanTof Fully TargetLynx Compatible Alprazolam in PPT Plasma 3x Dev <20 at LOQ Dev <15 other

33 Alprazolam Quantitation Curve Absolute, Internal Standards: R 2 = R 2 = pg/ml 20 pg/ml to 200 ng/ml 200,000 pg/ml Relative Response Factors: Via Orthogonal Detection (UV, RAD)

34 Summary Xevo TQ-S and Xevo G2 QTof provide class leading instrument performance Innovative Technologies such as StepWave, ScanWave and QuanTof engineered to integrate into existing workflows and open paths to new workflows for comprehensive sample analysis and increased productivity LCMS Innovations to merge Quantitative and Qualitative analysis without compromising your analytical objectives

35 Acknowledgements Waters Corporation Paul Rainville Marian Twohig Joanne Mather Rob Plumb Kate Yu Henry Shion Steve McDonald Gordon Fujumoto Alan Millar Jeff Goshawk Alex Hooper Hoffman-LaRoche Zhenmin Liang Astra Zeneca, UK David Higton Dotmatics, LTD

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