IDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF METHANOL

Size: px
Start display at page:

Download "IDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF METHANOL"

Transcription

1 Open Access Research Journal Medical and Health Science Journal, MHSJ ISSN: (Print) (Online) Volume 12, 2012, pp IDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF METHANOL EXTRACT OF LUFFA ACUTANGULA ROXB. Antibacterial activity of Luffa acutangula Roxb. (angled luffa) has been assayed against some pathogenic bacterial. Fruit powder of angled luffa was macerated with methanol, and the methanol ed sequentially with hexane, chloroform, ethyl acetate and buthanol. Antibacterial activity was evaluated by well diffusion method. Extract with the highest antibacterial activity was identified regarding their class of compounds using phytochemical screening and Thin Layer Chromatography (TLC) method. The antibacterial activity of the was compared with that of the ampicillin used. The methanol inhibited the growth of the P. aeruginosa, E. coli, B. subtilis and S.aureus, but did not inhibit the growth of the E. aerogenes, S. dysentriae and S. thypi. The ethyl acetate showed the highest antibacterial activity against P. aeruginosa, E. coli, B. subtilis and S. aureus, followed by chloroform, buthanol, and hexane, respectively. The ethyl acetate possed phenolic, condensed tannin, flavonoids, saponins and terpenoids. Based on the MIC and the equivalent value of ethyl acetate compared with that ampicillin used, the antibacterial activity of ethyl acetate was lower than with that of the ampicillin used. RESMI MUSTARICHIE 1, LINAR ZALINAR UDIN 2, MUCHTARIDI 1, SUPRIYATNA 1 1 Faculty of Pharmacy, Universitas Padjadjaran, Indonesia 2 Department of Applied Chemistry, Indonesian Institute of Sciences, Indonesia Keywords: Luffa acutangula Roxb., antibacterial activity, well diffusion, methanol, ethyl acetate. UDC: Introduction The problems of unwanted side effects of some antibiotics and the urgent need to cure infectious diseases induce scientists to search for new antibacterial drugs derived from plants (Aliero, Aliero, and Buhari, 2008). Cucurbitaceae plants tribes that have been reported among other things provide antibacterial activity of Luffa cylindrica (= Belustru) and Benincosa hispida (= Beligo). Oyetayo et al. (2007) states that the leaves and fruit seeds contain antibacterial compounds of Belustru alkaloids and saponins. Kumar, Swamy, Sanjay et al. (2006) on the other hand reported that fruit Beligo methanol containing triterpenoids and flavonoids could inhibit bacterial growth. In this paper, we report testing the antibacterial activity of methanol of Luffa acutangula Roxb. (angled luffa). This test includes identification of the phytochemical screening and testing of in-vitro antibacterial by hole diffusion method against E. coli, B. subtilis, S. aureus, P. aeruginosa, S. dysentriae and/or S. thypi and Ampicillin was used as the MIC appeal against antibacterial activity. Experimental Materials and instruments All chemicals were used as received without further purification and all solvents were of reagent grade. Test bacteria used were E. coli FNCC 0091, B. subtilis FNCC 0059, S. aureus FNCC 0047, P. aeruginosa FNCC 0063 and S. thypi FNCC E. aerogenes and E. dysentriae

2 were obtained from Indonesian Institute of Sciences - Bandung. One-way ANOVA with p> 0.05 were applied for data analysis. Flow chart of the experiment is shown in Figure 1. FIGURE 1. FLOW CHART OF THE RESEARCH Angled luffa fruit (1000 g) Dried powder Thinly sliced, dried in an oven for 3 days 55 o C then finally milled Test for antibacterial activity against E.coli, B.subtilis, S. aureus, P. aeruginosa, E. aerugenes, S. dysentriae and S. thypi 1. Macerated with 2.5 L methanol 1x 24 hours, and succesvely by 3 x 30 hours with 850, 990 dan 600 ml methanol. 2. Evaporated by Rotary Evaporator at 40 o C Concentrated methanol (261.6 g) Added 200 ml water:metanol (1:3) Aqueous methanol 1. Multilevel solvent ion using successive hexane, chloroform, ethyl acetate and butanol. 2. Evaporated by Rotary evaporator at 40 oc n-hexane Chloroform Ethyl acetate Butanol Residue/wat er Phytochemical screening: saponin, phenolate, tannin / polyphenol, flavonoid, terpenoid and alkaloid Tests for antibacterial activity against bacteria that can be inhibited by the methanol Antibacterial Determination of antibacterial the highest by comparing the inhibition diameter formed Determination of minimum inhibitory concentration and value appeal to ampicillin The highest antibacterial Confirmation test compound class with the TLC

3 Results and discussion Maceration Medical and Health Science Journal / MHSJ / ISSN: (Print) (Online) Maceration method used as ion method provided quite a lot ion and reduced the excessive heat of the samples. Therefore, not many damaged ed compounds conditioned use of methanol as a solvent; all the compounds of plants suspected of being the compound are compound antimicroorganisms dissolved in organic solvents, so that these compounds can be obtained through solvent ion with methanol (Cowan, 1999). It is known that methanol can almost all the compounds including anthocyanin compounds, terpenoids, saponins, lactone, tannins, flavones, and polyphenols. Durmaz, Sagun, Tarakci, and Ozgokce (2006) showed that ion with methanol is a good method for ing plant antibacterial compounds from Allium vineale Linn., Chaerphyllum macropodum Linn. and Prangos ferulacea (L) Lindl. Multiple soaking was useful to optimize the ion of the compounds contained in samples. Tests for antibacterial activity of methanol s Tests for antibacterial activity of methanol s are shown in Table 1. Tests conducted by the hole diffusion method using DMSO as solvent. DMSO is also used as a negative control, since DMSO is a colorless aprotic polar solvent that can dissolve polar and non- that the methanol s inhibited the polar with a very wide range. Table 1 shows growth of E. coli, S. aureus, P. aeruginosa and B. subtilis, but did not inhibit bacterial growth of E. aerogenes and S. dysentriae. Repeated testing with two different concentrations as shown in Figure 2 confirms these results wheree for S. thypi there was not observed growth inhibition. TABLE 1. TESTS FOR ANTIBACTERIAL ACTIVITY OF METHANOL EXTRACT OF LUFFA FRUIT No. Tested Bacteria Test results Sample weight 20 mg/hole Sample weight 15 mg/hole Sample weight 10 mg/hole 1 S. aureus * P. aeruginosa E. coli B. subtilis + * + 5 E. aerogenes S. dysentriae S. thypi * * * Note: (+) - positive anti bacterial; (-) - negative antibacterial; (*) - diameter of inhibition is not so clear (dubious) Effect of variation of bacteria in each sample weight and influence of the increasing weight of each sample to bacteria growth inhibition can be detected by data analysis using One-way ANOVA. This study shows that the effect of weight variation test sample generally provides the effect of bacterial variation in inhibiting the growth of bacteria; however, bacteria S.aureus with P. aeruginosa, B. subtilis with S. aureus, P. aeruginosa with B. subtilis gave similar effects in inhibiting the growth of tested bacteria. Effect of increasing the weight of each sample of bacteria showed that increasing sample weight of methanol s from 10 mg/hole to 15 mg/hole had no effect in inhibiting the growth of bacteria S. aureus, E. coli and B. subtilis, but gave effect in inhibiting the growth of bacteria P. aeruginosa marked with significant differences between sample weight 10 mg/hole with a sample weight 15 mg/sample

4 FIGURE 2. ANTIBACTERIAL ACTIVITY TEST RESULTS OF THE METHANOL EXTRACT OF THE BACTERIUM S. AUREUS, P. AERUGINOSA, E. COLI, B. SUBTILIS AND S. THYPI Diameter of Inhibition (MM) mg/hole 15mg/hole S.aureus P aeruginosa E coli B subtilis S.typhi The above results show that the methanol s inhibited the growth of gram positive bacteria (B. subtilis and S. aureus) and gram negative bacteria (P. aeruginosa and E. coli), and the obtained results are consistent with results from Tomori et al. (2007) showed that the ethanol of the fruit L. breviflora (also Cucurbitaceae) inhibits the growth of the bacterium E. coli, B. subtilis, S. aureus, and P. aeruginosa Tests for antibacterial activity of s from various solvents As shown in the Figure 1, the methanol was ed using a solvent gradually increasing polarity. The was then tested for antibacterial activity (Figure 3). FIGURE. 3 ANTIBACTERIAL ACTIVITY TEST RESULTS OF VARIOUS SOLVENT EXTRACTS OF THE BACTERIUM S. AUREUS, P. AERUGINOSA, E. COLI, AND B. SUBTILIS WITH EXTRACT WEIGHT OF 15 MG/HOLE 14 diameter of inhibition (mm) E coli B subtilis S aureus P aeruginosa hexane chloroform n butanol ethyl acetate water

5 Extracts which had the highest bacterial activity included ethyl acetate followed by chloroform, butanol and hexane. Water s had no antibacterial activity and were marked by the lack of inhibition diameter around the hole. Testing antibacterial active compounds To identify groups of compounds contained in s, chemical screening was carried out with the result shown in Table 2. TABLE 2. THE TEST RESULTS ANTIBACTERIAL COMPOUNDS CONTAINED IN THE VARIOUS EXTRACTS No. Group Test results Compound Methanol Hexane Chloroform Ethyl acetate Butanol tested Extract 1 Alkaloid Phenolic Saponin Tannin* Flavonoid Terpenoid Note: * condensed tannin, (+)= test positive, (-) = test negative As shown in Table 2, hexane which contains only terpenoid compounds has antibacterial activity against all tested bacteria but smallest compared to other s. This indicates that the terpenoid group has contributed in inhibiting the growth of E. coli, B. subtilis, S. aureus, and P. aeruginosa with little strength. The phytochemical screening of the fractions of ethyl acetate was followed by TLC mobile phase chloroform test (Table 3). TABLE 3. TEST RESULTS OF GROUPS OF COMPOUNDS IN THE EXTRACT OF ETHYL ACETATE AND ITS TLC AND PS RESULTS Group PS TLC results Compound results Visible UV 254 nm UV 365 nm Tested Rf Colour Note Rf Colour Note Rf Colour Note Flavonoid Tawny Yellowish + Green 0.39 Greenish + Yellow Tannin & Grayish Grayish Grayish + Phenolic Black Black Black Terpenoid Purple + Saponin Greenish + Blue 0.55 Blue + Note : (+) Test positive, PS = Phytochemical Screening, TLC = Thin Layer Chromatography Cowan (1999) states that alkaloids have antibacterial activity associated with high aromatic compound content which have contributed to form a chelate with bacteria DNA. Flavonoids have pharmacological activity associated with the ability to work as a powerful

6 antioxidant, free radical catchers, chelate with metal forming and interacting with the enzyme (Bylka, Matlawska, and Pilewski, 2004) and flavonoids also. Tannin has antibacterial activity through molecular action, i.e. by forming complexes with proteins through hydrogen bonding and hydrophobic bonding, binding of bacterial peptidoglycan cell wall and inhibiting the activity of the enzyme lactase which is a b- destroying enzyme b-lactam antibiotics (Shimamura, Zhao, and Hu, 2007). Saponins can act as detergents that have a structure binding of hydrophilic molecules and organic molecules to damage the cytoplasmic membrane and kill the bacteria. Terpenoids have antibacterial activity associated with the destruction of the cell membrane by lipophilic compounds. Phenolic compounds can cause denaturation of proteins through adsorption processes involving hydrogen bonds. Comparative antibacterial activity of ethyl acetate with ampicillin Ampicillin is used as a comparator antibacterial activity of the ethyl acetate. The test result of minimum inhibitory concentration (MIC) of ethyl acetate s is shown in Figure 4. FIGURE 4. THE TEST RESULTS OF MINIMUM INHIBITORY CONCENTRATION OF ETHYL ACETATE EXTRACT diameter of inhibition (mm) E coli B Subtilis S aureus P aeruginosa 0,75mg/micro L 0,30mg/micro L 0,1 mg/micro L 0,28mg/micro L The smallest concentration used was mg/µl which can still inhibit the growth of all bacteria. Table 4 is obtained by using concentrations of 0.10 mg/µl or sample weight 2.0 mg/hole up to mg/µl or sample weight 0.20 mg/hole. Ampicillin inhibitory concentration test results are shown in Table 5. Results of comparative determination of ethyl acetate and aampicillin are shown in Table 6. It can be concluded that the antibacterial activity of ethyl acetate from the fruit of Luffa is weaker when compared with ampicillin; because the ethyl acetate has a minimum inhibitory concentration greater than the MIC for ampicillin, that is E.coli is 0.003%, B. subtilis %, S. aureus %, and P. aeruginosa %

7 TABLE 4. THE MIC OF ETHYL ACETATE EXTRACT Concentrations Inhibition diameter (mm) mg/µ L E.coli B. subtilis S. aureus P. aeruginosa ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.00 Note : Inhibiton diameter of negative control 6 mm TABLE 5. THE MIC OF AMPICILLIN Concentrations Inhibition diameter (mm) mg/µ L E.coli B. subtilis S. aureus P. aeruginosa ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.00 Note : Inhibiton diameter of negative control 6 mm Bacteria TABLE 6. THE RESULT OF THE COMPARATIVE DETERMINATION OF ETHYL ACETATE EXTRACT AND AMPICILLIN The actual concentration of ethyl acetate The equivalent concentration of ethyl acetate to Ampicillin The comparative value against Ampicillin E. coli 0.10 mg/µ L mg/µ L % B. subtilis 0.10 mg/µ L mg/µ L % S. aureus 0.10 mg/µ L mg/µ L % P. aeruginosa 0.10 mg/µ L mg/µ L % Conclusion Luffa fruit methanol inhibited the growth of B. Subtilis, S. Aureus, P. aeruginosa and E. Coli, but did not inhibit the growth of E. Aerogenes and S. Thypi. Extracts which had the highest antibacterial activity was ethyl acetate followed by chloroform, butanol and hexane s. Chemical screening results obtained from the ethyl acetate did not contain a class of alkaloid compounds, chloroform s did not contain the saponin compound, butanol did not contain saponins and terpenoid compounds and hexane s contained only terpenoid compounds. Antibacterial activity of ethyl acetate luffa fruits is weaker when compared to Ampicillin

8 References Aliero, A., Aliero, B., and Buhari, U., Preliminary phytochemical and antibacterial screening of Scadoxus multiflorus," Int. Jor. P. App. Scs., Vol.2(4), pp Bylka, M., Matlawska, I., Pilewski, N., Natural flavonoids as antimicrobial agents, JANA, Vol.7(2), pp Cowan, M., Plant products as antimicrobial agents, Clinical Microbiology Reviews, Vol.12(4), pp Durmaz, H; Sagun, E; Tarakci, Z; Ozgokce, F., Antibacterial activities of Allium vineale, Chaerophyllum macropodum and Prangos ferulacea, African Journal of Biotechnology, Vol.5(19), pp Kumar, G., Swamy, V.B., Sanjay, P. And Kumar, A., Antimicrobial effect of Benincasa hispida against acne inducing bacteria, AAPSJ, Abstratct retrived in August 22, 2012 at abstracts/am_2006/staged/aaps pdf Oyetayo, F., Oyetayo, V., and Ajewole, V., Phytochemical profile and antibacterial properties of the seed and leaf of the Luffa plant (Luffa cylindrica), J. Pharmacol. Toxicol., Vol. 2(6), pp Shimamura, T., Zhao, W., and Hu, Z., Mechanism of action and potential for use of tea catechin as an antiinfective agent, Anti-infective Agent In Medicinal Chemistry, Vol.6, pp Tomori, O., Saba, A., and Dada-Adegbola, H., Antibacterial activity of ethanolic of whole fruit of Lagenaria breviflora Robert, J. Anim.Vet.Adv., Vol.6(5), pp

Jl. Perintis Kemerdekaan KM.5 Makassar 90231, South Sulawesi Indonesia.

Jl. Perintis Kemerdekaan KM.5 Makassar 90231, South Sulawesi Indonesia. ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com ANTIMICROBIAL POTENCY OF PASSIFLORA FOETIDA LINN FROM SOUTH SULAWESI INDONESIA AGAINST BACTERIA IN VITRO Andi

More information

Chandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp.

Chandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp. RESEARCH ARTICLE OPEN ACCESS Antibacterial properties of Unripened Fruits of Trapa natans, Annona squamosa, Ficus carica and Anthocephalus cadamba against Pathogenic Gram Positive and Negative Bacteria.

More information

In vitro study of antibacterial activity of Carissa carandas leaf extracts

In vitro study of antibacterial activity of Carissa carandas leaf extracts Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (1):36-40 ISSN : 2249-7412 CODEN (USA): AJPSKY In vitro study of antibacterial activity of Carissa

More information

Phytochemical analysis and antibacterial properties of leaf extract of Azima tetracantha (Lam.)

Phytochemical analysis and antibacterial properties of leaf extract of Azima tetracantha (Lam.) Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (2):110-114 ISSN : 2249-7412 CODEN (USA): AJPSKY Phytochemical analysis and antibacterial properties

More information

Chapter 4 Solvent-solvent separation

Chapter 4 Solvent-solvent separation Chapter 4 Solvent-solvent separation 88 Chapter 4 Solvent-solvent separation Abstract The skilful application of fractionation methods exploits the fact that an extract can be separated into groups of

More information

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS OF Saccharum officinarum Linn (Ikshu) ROOTS

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS OF Saccharum officinarum Linn (Ikshu) ROOTS International Journal of Science, Environment and Technology, Vol. 5, No 4, 2016, 2315 2322 ISSN 2278-3687 (O) 2277-663X (P) PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DIFFERENT

More information

Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography

Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography Middha Himanshu 1* and Parihar Pradeep 2 1Department of Microbiology, DTM College of Biosciences, Bikaner, Rajasthan 2 Lovely Professional

More information

ANTIMICROBIAL ACTIVITY OF NON EDIBLE SEEDS AGAINST IMPORTANT PATHOGENIC MICROORGANISMS PROJECT REFERENCE NO.: 38S _B_MSC_010

ANTIMICROBIAL ACTIVITY OF NON EDIBLE SEEDS AGAINST IMPORTANT PATHOGENIC MICROORGANISMS PROJECT REFERENCE NO.: 38S _B_MSC_010 ANTIMICROBIAL ACTIVITY OF NON EDIBLE SEEDS AGAINST IMPORTANT PATHOGENIC MICROORGANISMS PROJECT REFERENCE NO.: 38S _B_MSC_010 COLLGE BRANCH GUIDE STUDENTS : UNIVERSITY OF MYSORE, MYSORE : DEPARTMENT OF

More information

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.7, No.4, pp ,

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.7, No.4, pp , International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 974-44 Vol.7, No.4, pp 6-65, 4-5 Burn Wound Healing Effect of Trembesi (Samanea saman) Leaves Extract Gel on Rats (Rattus novergicus)

More information

ANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS

ANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS ANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS Ajima Karphrom 1, Suthisak Suknaisilp 2, Pasawadee Pradeepasaena 2, Sumate Tantratian 2 * ABSTRACT Seeds of betel nuts grown in

More information

ISSN Volume 2 (2015) The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013)

ISSN Volume 2 (2015) The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013) ISSN 2413-0877 Volume 2 (2015) 129-133 The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013) DETECTION OF ALKALOID, FLAVONOID, AND TERPENOID COMPOUNDS IN BREAD (Artocarpus communis

More information

Antimicrobial activity of leaf extract of Ocimum sanctum on various clinical isolates

Antimicrobial activity of leaf extract of Ocimum sanctum on various clinical isolates Microbiology- Original article DOI: http://dx.doi.org/10.18320/jimd/201603.0291 JOURNAL OF INTERNATIONAL MEDICINE AND DENTISTRY To search..to know...to share p-issn: 2454-8847 e-issn: 2350-045X Antimicrobial

More information

QUANTITATIVE ESTIMATION OF PHYTOSTEROL FROM TWO MEDICINALLY IMPORTANT PLANTS OF CUCURBITACEAE

QUANTITATIVE ESTIMATION OF PHYTOSTEROL FROM TWO MEDICINALLY IMPORTANT PLANTS OF CUCURBITACEAE Int. J. Engg. Res. & Sci. & Tech. 2014 Renu Sarin and Sangeeta Samria, 2014 Research Paper ISSN 2319-5991 www.ijerst.com Vol. 3, No. 2, May 2014 2014 IJERST. All Rights Reserved QUANTITATIVE ESTIMATION

More information

Sulistiyaningsih et al ISPST 2018 ISSN:

Sulistiyaningsih et al ISPST 2018 ISSN: EVALUATION OF ETHANOLIC EXTRACT AND FRACTIONS OF RAMBUTAN LEAVES (Nephelium lappaceum) AGAINST Shigella dysenteriae AND Bacillus cereus AS ANTI DIARRHEA AGENT Sulistiyaningsih 1*, Amita Putri Afini 1,

More information

Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts

Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts American Journal of Analytical Chemistry, 2016, 7, 254-259 Published Online March 2016 in SciRes. http://www.scirp.org/journal/ajac http://dx.doi.org/10.4236/ajac.2016.73022 Study of Phytochemical Screening

More information

ANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT.

ANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT. International Journal of Advanced Research and Review www.ijarr.in ANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT. Dr.Mayuri Thanwar 1, Dr.Dhananjay Dwivedi 2 1. Scientific Officer,

More information

Studies on the Antibacterial Activity of Quercus Infectoria Galls

Studies on the Antibacterial Activity of Quercus Infectoria Galls 2011 International Conference on Bioscience, Biochemistry and Bioinformatics IPCBEE vol.5 (2011) (2011) IACSIT Press, Singapore Studies on the Antibacterial Activity of Quercus Infectoria Galls T.Leela

More information

THIN LAYER CHROMATOGRAPHY

THIN LAYER CHROMATOGRAPHY THIN LAYER CHROMATOGRAPHY Thin layer chromatography is the best known technique of plant biochemistry. TLC is used for preliminary separation and determination of plant constituents. It is helpful for

More information

Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes

Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes ISSN: 2454-132X Impact factor: 4.295 (Volume3, Issue1) Available online at: www.ijariit.com Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes R. Prasanna PRIST University, Tamilnadu

More information

Octa Journal of Biosciences

Octa Journal of Biosciences Octa Journal of Biosciences ISSN 2321 3663 International peer-reviewed journal June 2014 Octa. J. Biosci. Vol. 2(1):59-63 Octa Journal of Biosciences journal homepage: www.sciencebeingjournal.com Antimicrobial

More information

Process Optimization for Preparation of an Antibacterial Agent Using a Combined Extract of Tulsi and Marigold Leaves

Process Optimization for Preparation of an Antibacterial Agent Using a Combined Extract of Tulsi and Marigold Leaves IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-issn: 319-40,p- ISSN: 319-399.Volume 11, Issue 5 Ver. I (May. 017), PP 31-35 www.iosrjournals.org Process Optimization

More information

6. SUMMARY AND CONCLUSION

6. SUMMARY AND CONCLUSION 6. SUMMARY AND CONCLUSION Infectious diseases are one of man s oldest enemies. They continue to be a serious burden around the world, in developing and industrialised countries alike. It is said that every

More information

Phytochemical screening and antibacterial properties of Garcinia kola

Phytochemical screening and antibacterial properties of Garcinia kola 2013; 2(3): 34-38 Online at: www.phytopharmajournal.com Research Article ISSN 2230-480X JPHYTO 2013; 2(3): 34-38 2013, All rights reserved Phytochemical screening and antibacterial properties of Garcinia

More information

SUBMISSION OF THE FINAL REPORT OF THE WORK DONE ON THE PROJECT

SUBMISSION OF THE FINAL REPORT OF THE WORK DONE ON THE PROJECT SUBMISSION OF THE FINAL REPORT OF THE WORK DONE ON THE PROJECT NAME OF THE PRINCIPAL INVESTIGATOR : Dr.V.M.Barot, NAME AND ADDRESS OF THE INSTITUTION : Smt.S.M.Panchal Science College, -383215,Gujarat

More information

International Journal of Pharma and Bio Sciences

International Journal of Pharma and Bio Sciences Research Article Microbiology International Journal of Pharma and Bio Sciences ISSN 0975-6299 PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF CLITORIA TERNATEA LEAVES AGAINST STAPHYLOCOCCUS AUREUS

More information

International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:

International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: Research Article CODEN: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com SYNERGISTIC ANTIBACTERIAL ACTIVITY OF CLOVE, CUMIN

More information

Dian Riana Ningsih, Zusfahair, Dwi Kartika. Chemistry Department Basic Science Faculty Jenderal Soedirman University. ABSTRACT

Dian Riana Ningsih, Zusfahair, Dwi Kartika. Chemistry Department Basic Science Faculty Jenderal Soedirman University. ABSTRACT In Vitro Antibacterial Test of Soursop (Annona muricata Linn) n-hexane Extract Leaves on Propionibacterium Acnes Dian Riana Ningsih, Zusfahair, Dwi Kartika Chemistry Department Basic Science Faculty Jenderal

More information

3. REVIEW OF LITERATURE

3. REVIEW OF LITERATURE 3. REVIEW OF LITERATURE Geissman TA. (1963) reported that the mostly traditional medicinal cum areomatic plants have an almost maximum power to synthesize aromatic substances because approx all aromatic

More information

In vitro Evaluation of Antibacterial Activity of Bark and Flower Extracts of Pimenta officinalis Lindl.

In vitro Evaluation of Antibacterial Activity of Bark and Flower Extracts of Pimenta officinalis Lindl. ADVANCES IN BIORESEARCH, Vol 1 [2] December 2010: 61-68 Society of Education, India http://www.soeagra.com ISSN 0976-4585 RESEARCH PAPER In vitro Evaluation of Antibacterial Activity of Bark and Flower

More information

Pharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L.

Pharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L. Pharmacognostic and preliminary phytochemical analysis of Aegle marmelos L. and Centella asiatica L. A. Vijayalakshmi, G. Sakthivigneswari and Suganya, J. Department of Botany, Avinashilingam Institute

More information

*MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN. Department of Botany, GC University, Lahore. ABSTRACT INTRODUCTION

*MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN. Department of Botany, GC University, Lahore. ABSTRACT INTRODUCTION BIOLOGIA (PAKISTAN) 01, 8 (1&), 17-17 PK ISSN 000-09 Antibacterial activity of crude s of different parts of Butea monosperma (Lamk.) Taub. *MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN Department of Botany,

More information

Keywords- Hibiscus rosa- sinensislinn., phytochemical, extract.antibacterial, axillary solitary, companulate.

Keywords- Hibiscus rosa- sinensislinn., phytochemical, extract.antibacterial, axillary solitary, companulate. GLOBAL JOURNAL OF ENGINEERING SCIENCE AND RESEARCHES COMPARATIVE PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF ETHANOLIC AND WATER FLOWER EXTRACTS OF HIBISCUS ROSASINENSIS LINN. (HIBISCUS RED)

More information

Jigna Parekh, Nehal Karathia and Sumitra Chanda*

Jigna Parekh, Nehal Karathia and Sumitra Chanda* African Journal of Biomedical Research, Vol. 9 (); 53-5 ISSN 1119 59 Ibadan Biomedical Communications Group Full Length Research Article Evaluation of antibacterial activity and phytochemical analysis

More information

ANTIBACTERIAL ACTIVITY OF LEAF AND SEED EXTRACTS OF DELONIX REGIA AND ACHYRANTHUS ASPERA AGAINST SELECTED BACTERIAL STRAINS

ANTIBACTERIAL ACTIVITY OF LEAF AND SEED EXTRACTS OF DELONIX REGIA AND ACHYRANTHUS ASPERA AGAINST SELECTED BACTERIAL STRAINS Int. J. Pharm. Med. & Bio. Sc. 2013 Dhanalakshmi D and Manimegalai K, 2013 Research Paper ISSN 2278 5221 www.ijpmbs.com Vol. 2, No. 2, April 2013 2013 IJPMBS. All Rights Reserved ANTIBACTERIAL ACTIVITY

More information

CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS

CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS 8.1 INTRODUCTION Medicinal plants are the backbone of traditional medicine and

More information

Research Article. Study on antibacterial activity of some medicinally important plants

Research Article. Study on antibacterial activity of some medicinally important plants Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 205, 7():2520-252 Research Article ISSN : 0975-78 CODEN(USA) : JCPRC5 Study on antibacterial activity of some medicinally

More information

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6.

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6. WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Neeru et al. SJIF Impact Factor 6.041 Volume 5, Issue 6, 1884-1891 Research Article ISSN 2278 4357 DETERMINATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID

More information

THE EFFECT OF ALKALOIDS AND FLAVONOIDS EXTRACTS OF VITEX DONIANA SEED ON SOME MICROORGANISMS

THE EFFECT OF ALKALOIDS AND FLAVONOIDS EXTRACTS OF VITEX DONIANA SEED ON SOME MICROORGANISMS THE EFFECT OF ALKALOIDS AND FLAVONOIDS EXTRACTS OF VITEX DONIANA SEED ON SOME MICROORGANISMS E.I. Okoye Department of Pure and Industrial Chemistry, Anambra State University, Uli, Nigeria ABSTRACT: This

More information

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.7, No.2, pp ,

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.7, No.2, pp , International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304 Vol.7, No.2, pp 238-242, 2014-2015 Cytotoxicity of Ekor naga Leaf (Rhaphidophora pinnata (Lf) Schott) Chloroform Extract

More information

ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR

ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR Sahaya Sathish S, Vijayakanth P, Palani R, Thamizharasi T and Vimala A Department of Botany, St. Joseph s College

More information

Pinus glabra - As a potential source of anti-mycobacterium tuberculosis agent: Phytochemical and antimicrobial studies of its stem extracts

Pinus glabra - As a potential source of anti-mycobacterium tuberculosis agent: Phytochemical and antimicrobial studies of its stem extracts Pinus glabra - As a potential source of anti-mycobacterium tuberculosis agent: Phytochemical and antimicrobial studies of its stem extracts I. O. Olanrewaju 1, J. O. Echeme 2, E. E. Adjere-Taunu 1, R.

More information

Comparative Phytochemical Evaluation of Mentha piperita Varieties (citrate and perpeta) Leaves and Stem Extracts

Comparative Phytochemical Evaluation of Mentha piperita Varieties (citrate and perpeta) Leaves and Stem Extracts Science Arena Publications Specialty Journal of Biological Sciences Available online at www.sciarena.com 2017, Vol, 3 (3): 1-5 Comparative Phytochemical Evaluation of Mentha piperita Varieties (citrate

More information

Antibacterial Activity of Francoeuria crispa, Pulicaria undulata, Ziziphus spina-christi and Cucurbita pepo Against Seven Standard Pathogenic Bacteria

Antibacterial Activity of Francoeuria crispa, Pulicaria undulata, Ziziphus spina-christi and Cucurbita pepo Against Seven Standard Pathogenic Bacteria Ethnobotanical Leaflets : 733. 09. Antibacterial Activity of,, Ziziphus spinachristi and Against Seven Standard Pathogenic Bacteria Hatil Hashim ElKamali 1* and Sana AlTayeb Mahjoub 2 1 Department of Botany,

More information

Life Science Archives (LSA)

Life Science Archives (LSA) Available online at www.jpsscientificpublications.com Life Science Archives (LSA) ISSN: 2454-1354 Volume 1; Issue - 3; Year 2015; Page: 161-165 Research Article In vitro STUDIES ON INHIBITORY EFFECT OF

More information

Available online at Scholars Research Library

Available online at  Scholars Research Library Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2017, 9 (2):74-78 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Anti-microbial Properties of Thai Traditional Flower Vegetable Extracts

Anti-microbial Properties of Thai Traditional Flower Vegetable Extracts Anti-microbial Properties of Thai Traditional Flower Vegetable Extracts Wunwisa Krasaekoopt and Areeya Kongkarnchanatip Faculty of Biotechnology, Assumption University Bangkok, Thailand Abstract In this

More information

Antibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn

Antibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn Antibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn Dhasarathan. P 1, Hemalatha. N 2, Theriappan. P 1 and Ranjitsingh, A.J.A 3 1 Department of Biotechnology, Prathyusha

More information

EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME EXTRACT

EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME EXTRACT International Journal of Allied Practice, Research and Review Website: www.ijaprr.com (ISSN 2350-1294) EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME

More information

Higher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991).

Higher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991). 4. ANTIMICROBIAL ACTIVITY OF PHYSALIS MINIMA L. 4.1. Introduction Use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicine

More information

Available online at Phytochemical analysis and inhibitory activity of Ornamental Plant (Bougainvillea spectabilis)

Available online at   Phytochemical analysis and inhibitory activity of Ornamental Plant (Bougainvillea spectabilis) Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 13, 3(2):1- ISSN : 2249-7412 CODEN (USA): AJPSKY Phytochemical analysis and inhibitory activity of Ornamental

More information

Antimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam

Antimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam Human Journals Research Article October 2016 Vol.:7, Issue:3 All rights are reserved by A. Lavanya et al. Antimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam Keywords:

More information

Screening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria

Screening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria Screening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria Original Article Digge V.G 1, Kuthar Sonali S 2, Hogade Maheshwar G 3,

More information

Antibacterial Terpenoid from The Bark of Lansium domesticum Corr cv. Kokossan (Meliaceae)

Antibacterial Terpenoid from The Bark of Lansium domesticum Corr cv. Kokossan (Meliaceae) ABSTRACT Antibacterial Terpenoid from The Bark of Lansium domesticum Corr cv. Kokossan (Meliaceae) Tri Mayanti, 1 Siti Soidah 1, W. Drajat Natawigena, 2 Unang Supratman, 1 dan Roekmi-ati Tjokronegoro 1

More information

The potential antioxidant activity of ethanolic extract of Aceh ant-plant (Mymercodia sp) on the free radical DPPH(1,1-Diphenyl-2-pikrylhidrazil)

The potential antioxidant activity of ethanolic extract of Aceh ant-plant (Mymercodia sp) on the free radical DPPH(1,1-Diphenyl-2-pikrylhidrazil) The potential antioxidant activity of ethanolic extract of Aceh ant-plant (Mymercodia sp) on the free radical DPPH(1,1-Diphenyl-2-pikrylhidrazil) 1 Suryawati, 2 Frengki, 1 Hijra Novia Suardi 1 Faculty

More information

Antioxidant Activity and Lipoxygenase Enzyme Inhibition Assay with Total Flavonoid Content from Garcinia hombroniana Pierre Leaves

Antioxidant Activity and Lipoxygenase Enzyme Inhibition Assay with Total Flavonoid Content from Garcinia hombroniana Pierre Leaves Pharmacogn J. 2017; 9(2): 267-272 A multifaceted peer reviewed journal in the field of Pharmacognosy www.phcogj.com www.phcog.net Original Article Antioxidant Activity and Lipoxygenase Enzyme Inhibition

More information

Standardization of Milagathi Choornam

Standardization of Milagathi Choornam 2014; 1 (6): 69-74 ISSN 2321-2187 IJHM 2014; 1 (6): 69-74 Received: 19-12-2013 Accepted: 16-01-2014 Priya F Government Siddha Medical College, Anna Hospital Campus, Shakila R Siddha Central Research Institute

More information

NEW JERSEY TEA, DRIED FOR HOMOEOPATHIC PREPARATIONS CEANOTHUS AMERICANUS SICCUM FOR HOMOEOPATHIC PREPARATIONS

NEW JERSEY TEA, DRIED FOR HOMOEOPATHIC PREPARATIONS CEANOTHUS AMERICANUS SICCUM FOR HOMOEOPATHIC PREPARATIONS NEW JERSEY TEA, DRIED FOR HOMOEOPATHIC PREPARATIONS CEANOTHUS AMERICANUS SICCUM FOR HOMOEOPATHIC PREPARATIONS Ceanothus americanus siccum ad praeparationes homoeopathicas Other Latin name used in homoeopathy

More information

Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin

Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin Indonesian Journal of Cancer Chemoprevention, 2016, 7(2): 49-53 ISSN: 2088 0197 Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin Laeli

More information

Aliphatic acids. Cycloaliphatic compounds. Lactone carboxylic acids. Polyketide pathway LICHEN. Shikimic acid. pathway

Aliphatic acids. Cycloaliphatic compounds. Lactone carboxylic acids. Polyketide pathway LICHEN. Shikimic acid. pathway LICHEN CHEMISTRY The secondary chemicals produced by lichens have attracted the attention of investigators for over 100 years, and information about their structure, biogenic origin and phylogenetic significance

More information

In vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae)

In vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae) Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2017, 9 [6]:28-34 [http://scholarsresearchlibrary.com/archive.html] ISSN 0975-5071 USA CODEN: DPLEB4

More information

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA CORCHORIFOLIA PLANT EXTRACTS

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA CORCHORIFOLIA PLANT EXTRACTS INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA

More information

ISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL

ISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL Indo American Journal of Pharmaceutical Research, 2013 ISSN NO: 2231-6876 Journal home page: http:///index.php/en/ INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH ISOLATION AND CHARACTERIZATION OF HESPERIDIN

More information

Phytochemical studies of Hybanthus enneaspermus Linn., F Muell.,

Phytochemical studies of Hybanthus enneaspermus Linn., F Muell., World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original

More information

Comparism of the Antimicrobial Activities of N-hexane and Diethyl Ether Extracts of Garcinia kola Heckel [Bitter kola] Seed

Comparism of the Antimicrobial Activities of N-hexane and Diethyl Ether Extracts of Garcinia kola Heckel [Bitter kola] Seed INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Comparism of the Antimicrobial Activities of N-hexane and Diethyl Ether Extracts of Garcinia kola Heckel [Bitter kola]

More information

Title: Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L.

Title: Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. Author's response to reviews Title: Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. Authors: Naima Saeed (naima_qau@hotmail.com) Muhammad

More information

International Journal of ChemTech Research CODEN (USA): IJCRGG ISSN: Vol.8, No.6, pp , 2015

International Journal of ChemTech Research CODEN (USA): IJCRGG ISSN: Vol.8, No.6, pp , 2015 International Journal of ChemTech Research CODEN (USA): IJCRGG ISSN: 0974-4290 Vol.8, No.6, pp 905-914, 2015 Antibacterial activity of Ethyl Acetate and Ethanol extract of Rhaphidophora pinnata (L.f) Schott

More information

A Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum

A Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum RESEARCH ARTICLE A Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum Fabiola 1, Dr. V. Judia Harriet Sumathy 2 PG & Research Department of

More information

International Journal of Pharma and Bio Sciences

International Journal of Pharma and Bio Sciences Research Article Biotechnology International Journal of Pharma and Bio Sciences ISSN 0975-6299 ANTIBACTERIAL ACTIVITY OF ALLIUM SATIVUM (GARLIC) AND IDENTIFICATION OF ACTIVE COMPOUND BY GC-MS ANALYSIS

More information

Life Science Archives (LSA)

Life Science Archives (LSA) Available online at www.jpsscientificpublications.com Life Science Archives (LSA) ISSN: 2454-1354 Volume 2; Issue - 2; Year 2016; Page: 514-518 Research Article ROLE OF Piper nigrum L. IN THE HEALTH CARE

More information

IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES

IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES Int. J. Chem. Sci.: 8(1), 2010, 559-564 IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES R. MEERA *, P. DEVI a, P. MUTHUMANI, B. KAMESWARI b and B. ESWARAPRIYA c Department

More information

Journal of Pharmacognosy and Phytochemistry. Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Extracts of Pisonea aculeata

Journal of Pharmacognosy and Phytochemistry. Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Extracts of Pisonea aculeata ISSN 2278-4136 ZDB-Number: 2668735-5 IC Journal No: 8192 Volume 2 Issue 1 Online Available at www.phytojournal.com Journal of Pharmacognosy and Phytochemistry Preliminary Phytochemical Screening and HPTLC

More information

CHAPTER 3 PHYTOCHEMICAL SCREENING AND ANTI-MICROBIAL ACTIVITY OF THE EXTRACTS OF SELECTED MEDICINAL PLANTS

CHAPTER 3 PHYTOCHEMICAL SCREENING AND ANTI-MICROBIAL ACTIVITY OF THE EXTRACTS OF SELECTED MEDICINAL PLANTS CHAPTER 3 PHYTOCHEMICAL SCREENING AND ANTI-MICROBIAL ACTIVITY OF THE EXTRACTS OF SELECTED MEDICINAL PLANTS 3.1. INTRODUCTION Medicinal plants are of important therapeutic aid for various ailments. Naturally

More information

COMMON BARBERRY FOR HOMOEOPATHIC PREPARATIONS BERBERIS VULGARIS FOR HOMOEOPATHIC PREPARATIONS

COMMON BARBERRY FOR HOMOEOPATHIC PREPARATIONS BERBERIS VULGARIS FOR HOMOEOPATHIC PREPARATIONS COMMON BARBERRY FOR HOMOEOPATHIC PREPARATIONS BERBERIS VULGARIS FOR HOMOEOPATHIC PREPARATIONS Berberis vulgaris ad praeparationes homoeopathicas Other Latin name used in homoeopathy : Berberis DEFINITION

More information

In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.)

In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.) INDIAN JOURNAL OF PHARMACEUTICAL & BIOLOGICAL RESEARCH (IJPBR). Vol. 1 (1), March., 13 In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.) G.Ramakrishnaiah * 1, T. Hariprasad

More information

Preliminary Phytochemical Evaluation and HPTLC Fingerprinting of Leaves of Azadirachta indica

Preliminary Phytochemical Evaluation and HPTLC Fingerprinting of Leaves of Azadirachta indica American Research Journal of Bio Sciences Original Article Volume 1, Issue1, 2015 Preliminary Phytochemical Evaluation and HPTLC Fingerprinting of Leaves of Azadirachta indica Sunita Dalal *, Leena Seasotiya

More information

A study on proximate analysis and antimicrobial properties of Bombax ceiba pentandra fruit and spike extracts

A study on proximate analysis and antimicrobial properties of Bombax ceiba pentandra fruit and spike extracts World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original

More information

*Corres.author: Mobile-: ,

*Corres.author: Mobile-: , International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.6, No.2, pp 881-885, April-June 2014 Production of Flavonoids from Terminalia arjuna (ROXB.) in vivo and in vitro Tissue

More information

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE PRELIMINARY PHYTOCHEMICAL ANALYSIS OF LEAF EXTRACTS OF DELONIX ELATA ARAVINDHAN THIRUPUTKUZHI RANGANATHAN 1, CHITRAA R CHANDRAN 1, PRADEEP

More information

Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts

Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 826-830 http://www.ijcmas.com Original Research Article Phytochemical Analysis and Antioxidant property of Aegle marmelos Extracts Anjay Kumar Gupta*, Sumeet

More information

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X

More information

Antibacterial Effect of Pulsatilla chinensis towards Staphylococcus aureus, Shigella dysenteriae, and Salmonella typhi

Antibacterial Effect of Pulsatilla chinensis towards Staphylococcus aureus, Shigella dysenteriae, and Salmonella typhi 292 AMJ June 2016 Antibacterial Effect of Pulsatilla chinensis towards Sim Chee Fong, 1 Yanti Mulyana, 2 Dolvy Girawan 3 1 Faculty of Medicine Universitas Padjadjaran, 2 Department of Microbiology Faculty

More information

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA SEED EXTRACT

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA SEED EXTRACT INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND INVITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA

More information

International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN

International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August-2016 105 Antimicrobial activity of Andrographis paniculata stem extracts. S.Gurupriya 1 and Dr.L.Cathrine 2 1 M.phil

More information

AMERICAN SPIKENARD FOR HOMOEOPATHIC PREPARATIONS ARALIA RACEMOSA FOR HOMOEOPATHIC PREPARATIONS

AMERICAN SPIKENARD FOR HOMOEOPATHIC PREPARATIONS ARALIA RACEMOSA FOR HOMOEOPATHIC PREPARATIONS AMERICAN SPIKENARD FOR HOMOEOPATHIC PREPARATIONS ARALIA RACEMOSA FOR HOMOEOPATHIC PREPARATIONS Aralia racemosa ad praeparationes homoeopathicas DEFINITION Dried, underground part of Aralia racemosa L.

More information

Pharmacologyonline 2: (2011) ewsletter Tiwari et al. A EVALUATIO OF A TIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CALE DULA OFFICI ALIS (LI.

Pharmacologyonline 2: (2011) ewsletter Tiwari et al. A EVALUATIO OF A TIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CALE DULA OFFICI ALIS (LI. A EVALUATIO OF A TIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CALE DULA OFFICI ALIS (LI.) *Prashant Tiwari 1, Ritesh Jain 1, Kuldeep Kumar 2, Rajnikant Panik 3, Pratap Kumar Sahu 4 1. School of Pharmacy,

More information

Phytochemical study and bioactivity of solvent extracts on Coriandrum sativum

Phytochemical study and bioactivity of solvent extracts on Coriandrum sativum International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com Volume 3, Issue 5-2016 Research Article Phytochemical study and bioactivity of solvent extracts on Coriandrum

More information

Enhanced antibacterial potential of ethanolic extracts of neem leaf (Azadiracta indica A. Juss.) upon combination with bacteriocin

Enhanced antibacterial potential of ethanolic extracts of neem leaf (Azadiracta indica A. Juss.) upon combination with bacteriocin ISSN: 2319-7706 Volume 3 Number 9 (2014) pp. 617-621 http://www.ijcmas.com Original Research Article Enhanced antibacterial potential of ethanolic extracts of neem leaf (Azadiracta indica A. Juss.) upon

More information

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS ISSN 2395-3411 Available online at www.ijpacr.com 238 Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS G. Jyoti Jain 1* and S. Ramachandra Setty 2 1 Department of Pharmacology,

More information

Oligomeric proanthocyanidin fractions from fresh tea leaves and Staphylococcus aureus

Oligomeric proanthocyanidin fractions from fresh tea leaves and Staphylococcus aureus J.Natn.Sci.Foundation Sri Lanka 2014 42 (3): 241-246 DI: http://dx.doi.org/10.4038/jnsfsr.v42i3.7397 RESEARCH ARTICLE ligomeric proanthocyanidin fractions from fresh tea leaves and Staphylococcus aureus

More information

Chapter III. Effect of sorghum crude phenolic extracts on Bacillus cereus, Escherichia coli and Listeria monocytogenes

Chapter III. Effect of sorghum crude phenolic extracts on Bacillus cereus, Escherichia coli and Listeria monocytogenes Chapter III Effect of sorghum crude phenolic extracts on Bacillus cereus, Escherichia coli and Listeria monocytogenes 46 3.1 Abstract Freeze-dried phenolic extracts obtained from defatted bran fractions

More information

Evaluation of Antibacterial and Phytochemical Analysis of Mangifera indica Bark Extracts

Evaluation of Antibacterial and Phytochemical Analysis of Mangifera indica Bark Extracts ISSN: 2319-7706 Volume 3 Number 5 (2014) pp. 567-580 http://www.ijcmas.com Original Research Article Evaluation of Antibacterial and Phytochemical Analysis of Mangifera indica Bark Extracts V.Gomashe Ashok,

More information

Bioactivity Screening of Crude Alkaloidal Extracts from Some Rubiaceae

Bioactivity Screening of Crude Alkaloidal Extracts from Some Rubiaceae CMU. Journal (2005) Vol. 4(1) 59 Bioactivity Screening of Crude Alkaloidal Extracts from Some Rubiaceae Dammrong Santiarworn 1, Saisunee Liawruangrath 2, Apiwat Baramee 2, Hiromitsu Takayama 3 and Boonsom

More information

Antifungal activity of methanolic and ethanolic leaf extracts of medicinal plants

Antifungal activity of methanolic and ethanolic leaf extracts of medicinal plants INTERNATIONAL JOURNAL OF PLANT PROTECTION VOLUME 9 ISSUE 2 OCTOBER, 216 474-478 e ISSN-976-68 Visit us : www.researchjournal.co.in IJPP RESEARCH PAPER DOI : 1.74/HAS/IJPP/9.2/474-478 Antifungal activity

More information

Keywords: antioxidant; extraction; paper flower; phenolic compound

Keywords: antioxidant; extraction; paper flower; phenolic compound PHENOLIC ANTIOXIDANTS FROM Bougainvillea SPP. Punyawatt Pintathong 1, *, Prisana Pinket 1, Monthira Papoodplook 1, Natthawut Thitipramote 1,2, Phanuphong Chaiwut 1 1 School of Cosmetic Science, Mae Fah

More information

Antimicrobial activity of Terminalia chebula

Antimicrobial activity of Terminalia chebula , ISSN 2249 4340 Vol. 1, No. 2, pp. 175-179, September 2011 RESEARCH ARTICLE Antimicrobial activity of Terminalia chebula M. Golam MOSTAFA, Mahdia RAHMAN, M. Manjurul KARIM* Department of Microbiology,

More information

The Study of Antioxidant and Antibacterial Properties of Skin, Seeds and Leaves of The Sri Lankan Variety of Pumpkin.

The Study of Antioxidant and Antibacterial Properties of Skin, Seeds and Leaves of The Sri Lankan Variety of Pumpkin. IOSR Journal Of Pharmacy (e)-issn: 2250-3013, (p)-issn: 2319-4219 Volume 8, Issue 2 Version. 1 (February 2018), PP. 43-48 www.iosrphr.org The Study of Antioxidant and Antibacterial Properties of Skin,

More information

Preparation and characterization of Aloe vera extract

Preparation and characterization of Aloe vera extract Chapter 2 Preparation and characterization of Aloe vera extract 2.1. INTRODUCTION Aloe vera ethanolic extract was prepared according to Noor et.al, 2008 with few modifications. The phytochemical analysis

More information

COCHINEAL FOR HOMOEOPATHIC PREPARATIONS COCCUS CACTI FOR HOMOEOPATHIC PREPARATIONS

COCHINEAL FOR HOMOEOPATHIC PREPARATIONS COCCUS CACTI FOR HOMOEOPATHIC PREPARATIONS COCHINEAL FOR HOMOEOPATHIC PREPARATIONS COCCUS CACTI FOR HOMOEOPATHIC PREPARATIONS Coccus cacti ad praeparationes homoeopathicas DEFINITION Whole, dried, female insect, Coccus cacti L. (Dactylopius coccus

More information

International Journal of Research in Pharmacy and Science

International Journal of Research in Pharmacy and Science Research Article Available online www.ijrpsonline.com ISSN: 2249 3522 International Journal of Research in Pharmacy and Science DPPH Scavenging Activity of Various Extracts of Sweet Potatoes Leaves with

More information

ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEAVES OF CANAVALIA MOLLIS WIGHT & ARN. (HORSE BEAN).

ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEAVES OF CANAVALIA MOLLIS WIGHT & ARN. (HORSE BEAN). Britto et al., IJPSR, 2011; Vol. 2(1): 95-101 ISSN: 0975-8232 IJPSR (2011), Vol. 2, Issue 1 (Research Article) Received on 14 August, 2010; received in revised form 12 November, 2010; accepted 26 December,

More information