Polymeric Sorbents for Sample Preparation

Transcription

1 Polymeric Sorbents for Sample Preparation Better Reproducibility Cleaner Extracts Faster Method Development Discover the benefits of strata -X

2 It s Absolute Magic! We re now using half the reagents, half the time, and half the price with triple results! We re getting chromatography that is sharp and clean, with improved resolution and recovery. This is a revolutionary product for analysis. Meredith Wilson, Dynacare Labs, AL, USA

3 strata-x polymeric sorbents! Faster Method Development Cleaner Extracts Better Reproducibility

4 Polymeric Sorbent Technology for Better SPE Most traditional solid phase extraction sorbents on the market are just variations of the same technology, and often result in poor analyte recoveries, insufficient cleanup, or irreproducibility from extraction to extraction. By contrast, strata-x sorbents have been developed using revolutionary polymeric technology and are designed to eliminate the common bottlenecks associated with traditional SPE sorbents. strata-x Polymer Surface modified Styrene divinylbenzene High Analyte Capacity Better Retention for Target Analytes Deconditioning ResistAnt Better Reproducibility From Extraction to Extraction Polymeric Surface ModifIcation Better Selectivity for Polar and Non-Polar Compounds strata- X strata-x- C strata-x- CW strata-x- AW Reversed phase Strong cation exchange & Reversed Phase Weak cation exchange & Reversed Phase Weak Anion Exchange & Reversed Phase

5 ) ) ) ) Faster Method Development With strata-x polymeric sorbents the guesswork that leads to lengthy method development and complications of screening multiple SPE sorbents is eliminated. Four unique selectivities have been developed to cover a diverse spectrum of analytes, and simplify the method development process for faster and efficient sample preparation. Sorbent Functional Group Mode Analyte strata-x )n Ṅ. O Reversed Phase Polar and Non-Polar strata-x-c )n O S O O - Reversed Phase & Strong Cation Exchange Bases strata-x-cw )n O OH Reversed Phase & Weak Cation Exchange Bases (including Quaternary Amines) strata-x-aw )n NH NH 2 Reversed Phase & Weak Anion Exchange Acids

6 Consistent Sample Processing and Automation strata-x polymeric sorbents are deconditioning resistant and will not lose selectivity after the sorbent bed runs dry. This results in consistent sample processing, preventing variable recoveries, which occurs on traditional SPE sorbents, as a result of the sorbent becoming deconditioned when processing samples on a vacuum manifold or during 96-well plate automation strata-x Sorbents High Recoveries % Recovery Si-based Conventional C Drying time (min) Doxepin Ranitidine Caffeine Theophylline Procainamide Conditions: Tubes: 30 mg/1 ml strata-x, 100 mg/1 ml Strata C18-E Sample: Analytes spiked in phosphate buffer saline (PBS) solution at concentration of 1 µg/ml, n=3 Method: The strata-x method was used. After the conditioning step the sorbent was dried under vacuum up to 10 min at 10 Hg. Percent recoveries were determined by HPLC analysis.

7 Better Reproducibility from Extraction to Extraction A carefully controlled manufacturing process combined with deconditioning resistant sorbent bed, strong retention mechanisms ensure that strata-x sorbents provide better reproducibility from extraction to extraction in comparison to traditional SPE sorbents. Theophylline Caffeine Batch Batch Batch Batch Batch Batch Batch Batch % Recovery % Recovery Conditions: Tubes: 30 mg/1 ml Method: Analytes were spiked in PBS solution at the concentration of 1 µg/ml. The strata-x method was used. Contact Phenomenex for method details.

8 Successful Cleanup! Rat bile was successfully cleaned up for LC-MS analysis using strata-x 200 mg cartridge. Approximately 90 % of semi-polar metabolites were recovered. Herve Aloysius, Merck, NJ, USA

9 Selective Retention for Target Analytes! strata-x: Reversed Phase pp strata-x-aw: Mixed-mode Weak Anion Exchange p. 15 strata-x-c: Mixed-mode Strong Cation Exchange p. 14 strata-x-cw: Mixed-mode Weak Cation Exchange pp

10 10 strata- X for Reversed Phase Enhanced Polar and Aromatic Selectivity strata-x is a surface modified styrene divinylbenzene polymer, which gives an SPE sorbent that has superior H-bonding and p-p bonding capabilities for enhanced retention of polar and aromatic analytes. This can be very powerful for applications that require simultaneous extraction of polar analytes (Log P<3) and non-polar parent compounds (Log P>3) B A % Recovery Prednisolone Betamethasone Betamethasone Valerate CN Extraction of Polar Steroids from Plasma (Conditions): Sorbent: strata-x 30 mg/1 ml Part No.: 8B-S100-TAK Condition: 1 ml methanol Equilibrate: 1 ml water Load: 1 ml porcine spiked with analyte Wash: 1 ml 5 % methanol in water Elute: 1 ml methanol Evaporate: add 25 µl of 0.1 mg/ml butyl paraben (external standard) dry down under slow stream of N 2 and reconstitute in 200 µl acetonitrile HPLC Column: Synergi Max-RP 4 µm 150 x 4.6 mm SecurityGuard C18 4 x 3.0 mm Part No.: 00F-4337-E0 and AJ Sample: 50 µl of reconstituted extract Mobile Phase: A: 20 mm KH 2 PO 4 (ph 7.0); B: methanol Isocratic: A/B (35:65) for 20 min Flow Rate: 1.0 ml/min Temperature: 25 C Detector: 254 nm Peaks: 1. Prednisolone (1.0 µg/ml) 2. Betamethasone (1.0 µg/ml) 3. Butyl Paraben (external std) 4. Betamethasone Valerate (4.0 µg/ml) Chromatogram of Extracts: A) Blank B) Spiked Sample

11 strata- X for Reversed Phase Cleaner Extracts from Biological Sample Matrices 11 The strong multimode retention mechanisms of strata-x allows for aggressive washing with organic solvents without dramatically affecting recovery of analytes. This is exceptionally useful for completely removing hydrophobic contaminants from biological sample matrices found in urine or plasma. % Analyte Loss Less than 15 % of analyte lost when washed with 50 % methanol on strata-x! % Methanol in Water strata-x Waters Oasis Atenolol Wash Study (Log P = 0.16) (Conditions): Sorbents: strata-x and Oasis HLB 30 mg/1 ml Condition: 1 ml methanol Equilibrate: 1 ml water Load: 1 ml of spiked Atenolol (40.0 µg/ml) in water (ph 5.0) Wash: 1 ml volumes of 30, 40, and 50 % methanol in de-ionized water (see graph), to collected fractions add 100 µl of Propranolol (100 µg/ml) for HPLC analysis. HPLC Column: Luna 5 µm C8(2), 150 x 4.6 mm Part No.: 00F-4249-E0 Mobile Phase: A: 20 mm KH 2 PO 4 (ph 7.0); B: acetonitrile Gradient: A/B (90:10) to A/B (40:60) in 5 minutes hold for 6 minutes Flow Rate: 1.0 ml/min Temperature: 25 C Detector: 214 nm Sample: Atenolol In this comparison test of SPE tubes, strata-x has stronger retention for the polar compound Atenolol.

12 12 strata- X-CW for Weak Cation Exchange Elution of Quaternary Amines Weak cation exchange functionality of strata-x-cw allows for elution of strong bases such as quaternary amines that are irreversibly retained on strong cation exchangers. The pka of the strata-x-cw sorbent is more versatile in comparison to strong cation exchangers, giving a wider range of flexibility for elution conditions. No Dry Down Needed 100 Plasma Extracts (LC/MS extracted ions m/z; 304(C12) and 332(C14)) TIC + - All Elute Fraction 80 EIC All EIC All % Recovery C12 Benzalkonium Chloride C14 Benzalkonium Chloride CN Extraction of Benzalkonium Chloride from Plasma (Conditions): Sorbent: strata-x-cw 30 mg/1 ml Part No.: 8B-S035-TAK Condition: 1 ml methanol Equilibrate: 1 ml water Load: Analyte spiked human plasma (350 µl plasma µl water and acidify with 20 µl phosphoric acid) Wash 1: 1 ml 25 mm sodium acetate buffer ph 4.5, Dry for 1 min Wash 2: 1 ml methanol Elute: 1 ml 5 % formic acid/methanol Direct Inject: Direct injection of the elution fraction onto the LC-MS (no need to dry down) HPLC Column: Luna 5 µm CN 150 x 2.0 mm Part No.: 00F-4255-B0 Mobile Phase: A: 75 mm ammonium acetate (ph 5.0), B: acetonitrile Isocratic: A/B (40:60) Flow Rate: 0.2 ml/min MS Instrument: Bruker Esquire 2000 IT Source: ESI-Positive mode Samples: C12 Benzalkonium Chloride (100 ng/ml) C14 Benzalkonium Chloride (100 ng/ml)

13 strata- X-CW for Weak Cation Exchange Direct Inject of Analyte onto LC/MS 13 Analysis of basic compounds by LC-MS can be achieved with strata-x-cw. The functionality of strata-x-cw allows for elution of basic compounds in LC-MS compatible solvents for direct injection. Direct injection leads to faster analysis by removing time-consuming dry down and reconstitution steps. No Dry Down Needed A: Standard Solution B: Blank Plasma Extract C: Spiked Plasma Extract % Recovery Clidinium Bromide CN Extraction of Clidinium Bromide from Plasma (Conditions): Sorbent: strata-x-cw 30 mg/1 ml Part No.: 8B-S035-TAK Condition: 1 ml methanol Equilibrate: 1 ml water Load: Analyte spiked human plasma (350 µl plasma µl water and acidify with 20 µl phosphoric acid) Wash 1: 1 ml 25 mm sodium acetate buffer ph 4.5, Dry for 1 min Wash 2: 1 ml methanol Elute: 1 ml 5 % formic acid/methanol Direct Inject: Direct injection of the elution fraction onto the LC-MS (no need to dry down) HPLC Column: Luna 5 µm CN 150 x 2.0 mm Part No.: 00F-4255-B0 Mobile Phase: A: 75 mm ammonium acetate (ph 5.0), B: acetonitrile Isocratic: A/B (35:65) Flow Rate: 0.2 ml/min MS Instrument: Bruker Esquire 2000 IT Source: ESI-Positive mode Sample: Clidinium Bromide (50 ng/ml)

14 14 strata- X-C for Strong Cation Exchange Strong Retention of Basic Compounds strata-x-c has a strong cation exchange group uniformly bonded on the polymeric surface unlike traditional silica-based mixed mode sorbents that are blended, completely eliminating recovery or reproducibility problems. strata-x-c provides strong retention of basic compounds. The mixed mode mechanism can also be used to fractionate basic compounds from acidic and neutral impurities. 100 % organic wash results in clean extracts % Recovery B A min 20 0 Metoprolol Propranolol CN Extraction of Beta Blockers from Urine (Conditions): Sorbent: strata-x-c 30 mg/1 ml Part No.: 8B-S029-TAK Condition: 1 ml methanol Equilibrate: 1 ml water Load: 1 ml urine spiked with analytes; dilute 1:1 with phosphate buffer saline solution ph 7; acidified with 2 % phosphoric acid Wash 1: 1 ml 0.1 N HCI Wash 2: 1 ml methanol, Dry for 1 min Wash 3: 1 ml NH 4 OH/methanol/water (2:50:48) Elute: 1 ml 5 % NH 4 OH/methanol Evaporate: Spike extract with external standard (1 µg/ml); dry down under nitrogen and reconstitute with 200 µl 20 mm KH 2 PO 4 (ph 2.5) HPLC Column: Luna 5 µm C18(2), 150 x 4.6 mm Part No.: 00F-4252-E0 Sample: 50 µl of reconstituted extract Mobile Phase: A: 20 mm KH 2 PO 4 (ph 2.5); B: acetonitrile Gradient: A/B (95:5) to (50:50) in 15 minutes Flow Rate: 1.5 ml/min Temperature: 25 C Detector: 210 nm Peaks: 1. Pindolol (ext. std.), 2. Metoprolol (1.0 µg/ml) 3. Propranolol (1.0 µg/ml) Chromatogram of Extracts: A) Blank B) Spiked Sample

15 strata- X-AW for Weak Anion Exchange Selective Elution of Acidic Analytes 15 strata-x-aw can be used to retain acidic compounds. The weak anion exchanger of the strata- X-AW sorbent allows for the selective elution of compounds under acidic conditions or basic conditions for direct inject analysis onto a MS No Dry Down Needed min % Recovery Ortho-Phosphotyrosine CN Extraction of Ortho-Phosphotyrosine (Conditions): Sorbent: strata-x-aw 30 mg/1 ml Part No.: 8B-S038-TAK Condition 1: 1 ml methanol Condition 2: 1 ml formic acid/methanol/water (2:25:73) Equilibrate: 1 ml water Load: 1 ml 200 μg/ml in phosphate buffered saline/water (1:3) Wash 1: 1 ml water Wash 2: 1 ml methanol/water (50:50) Elute: 1 ml NH 4 OH/ methanol/water (5:50:45) Elution fraction 1:1 with water prior to injection (no need to dry down or reconstitute) HPLC Column: Gemini 5 μm C Å, 150 x 4.6 mm Part No.: 00F-4435-E0 Mobile Phase: 20 % acetonitrile/10 mm KH 2 HP0 4, ph 7.0 / 5 mm TBAC Injection: 10 μl Flow Rate: 1.0 ml/min Temperature: 30 C Detection: 220 nm Recovery Data: Sample % Recovery % RSD Ortho-Phosphotyrosine 99 3

16 16 X-SPEct More! Had many problems cleaning up dog plasma. Followed your elution procedure for strata-x. Remarkably clean. Very high analyte recovery. We will be switching to strata-x tubes. Howard Lanidis, Jacobus Pharmaceuticals, NJ, USA

17 17 Versatile SPE Applications Pharmaceutical pp Environmental pp Toxicology pp

18 18 Pharmaceutical strata- X On-Line for Reversed Phase On-Line Extraction Acyclovir (Log P -1.56) EIC % Recovery Acyclovir 40 ng/1 ml Acyclovir 20 ng/1 ml TN Analysis Conditions: Sorbent: strata-x 20 x 2.0 mm cartridge plus holder Part No.: 00M-S033-B0-CB Mobile Phase: A: water; B: acetonitrile Flow Rate: Pump 1-3 ml/min, cartridge; Pump 2-1 ml/min, analytical column Load: Pump % A (cartridge) Elution: Pump 2-80 % B: 20 % A (analytical column) HPLC Column: Luna 3 µm NH 2 50 x 3.0 mm Part No.: 00B-4377-Y0 Mobile Phase: 80 % B: 20 % A Flow Rate: 1.0 ml/min Detection: MS in ESI-Negative mode m/z Samples: Acyclovir (40 ng/1 ml); Acyclovir (20 ng/1 ml) Note: no buffers are used in this method

19 strata- X-CW for Weak Cation Exchange Extraction of Antihistamines Pharmaceutical 2 1 B: Spiked Sample A: Blank min % Recovery Chlorpheniramine Triprolidine CN Analysis Conditions: Sorbent: strata-x-cw 30 mg/1 ml Part No.: 8B-S036-TAK Condition.: 1 ml methanol Equilibrate: 1 ml water Load: 1 ml spiked urine (500 µl urine µl 100 mm sodium acetate buffer ph 4.5) Wash 1: 1 ml water Wash 2: 1 ml 100 mm sodium acetate buffer ph 4.5 Wash 3: 1 ml methanol, dry for 1 min Elute: 1 ml 2 % formic acid/methanol. Add 100 µl of diphenhydramine (20.0 µg/ml) Evaporate and Reconstitute: Evaporate under nitrogen to dryness & reconstitute with 200 µl mobile phase buffer HPLC Column: Synergi Max-RP, 4 µm, 150 x 4.6 mm Part No.: 00F-4337-E0 Sample: 50 µl of reconstituted human urine extract Mobile Phase: A: 20 mm KH 2 PO 4 (ph 2.5); B: acetonitrile Isocratic: 90:10 (hold for 1 min) to 75:25 in 3 min, then hold for 9 min Flow Rate: 1.5 ml/min Temperature: 25 C Detection: 230 nm Peaks: 1. Chlorpheniramine (1.0 µg/ml) 2. Triprolidine (1.0 µg/ml) 3. Diphenhydramine (2.0 µg/ml) (IS)

20 20 toxicology strata- X-C for Strong Cation Exchange Extraction of Methadone from Urine % Recovery B A min 20 0 Methadone CN Analysis Conditions: Sorbent: strata-x-c 60 mg/3 ml Part No.: 8B-S029-UBJ Condition: 2 ml methanol Equilibrate: 2 ml water Load: 3 ml urine spiked with analytes and IS (0.5 µg/ml); dilute 1:1 with 100 mm KH 2 PO 4 (ph 6); acidified with 2 % phosphoric acid Wash 1: 2 ml 0.1 N HCI Wash 2: 2 ml methanol Elute: 2 ml 5 % NH 4 OH/methanol Evaporate: Dry down and reconstitute with 200 µl mobile phase HPLC Column: Synergi Max-RP 4 µm 150 x 4.6 mm Part No.: 00F-4337-E0 Sample: 50 µl of reconstituted urine extract Mobile Phase: A: 0.1 % TFA in water ph (2.1) B: acetonitrile Isocratic: A/B (65:35) Flow Rate: 1.0 ml/min Temperature: 25 C Detector: 210 nm Peaks: 1. Estazolam (IS) 2. Methadone (0.5 µg/ml) Chromatogram of Extracts: A) Blank B) Spiked Sample

21 strata- X-C for Strong Cation Exchange Extraction of THC-COOH from Urine Peaks: THC-COOH-d 3 (IS) THC-COOH (analyte) min A B % Recovery THC-COOH toxicology CN Analysis Conditions: Sorbent: strata-x-c 60 mg/3 ml Part No.: 8B-S029-UBJ Condition: 2 ml methanol Equilibrate: 2 ml 0.1 N HCI Load: Base hydrolysis: 2 ml urine spiked with analyte and IS, add 100 µl 10 N KOH. Mix, vortex and hydrolyze for 20 min at 60 C. Cool and adjust ph to (by adding 200 µl glacial acetic acid) Wash 1: 2 ml 0.1 N HCI Wash 2: 2 ml 0.1 N HCl/acetonitrile (70:30) Dry for 4 min under vacuum. To further remove water: add 200 µl glacial acetic acid, letting it percolate under gravity for sec. Dry sorbent for 1 min under vacuum Elute: 2 ml 2 % glacial acetic acid/acetonitrile Evaporate: under nitrogen and reconstitute with 30 µl 10 mm imidazole/ ethylacetate + 30 µl BSTFA (1 % TMCS). React at 70 C for 20 min. GC Column: Zebron ZB-5 Dimension: 15 m x 0.25 mm x 0.25 µm Part No.: 7EG-G Injection: 3 µl splitless for 0.4 min at 270 C Carrier Gas: Helium constant flow at 1.8 ml/min Oven Program: 160 C to 235 C at 20 C/min to 250 C at 3 C/min to 340 C at 25 C/min Detector: SIM mode (371, 374*, 473, 476*, 488, and 491*); source=240 C; Transfer line=300 C. * from d 3 Internal Standard (IS) Sample: THC-COOH (7.0 ng/ml) Chromatogram of Extracts: A) Blank B) Spiked Sample

22 22 environmental strata- X for Reversed Phase Extraction of Urea Pesticides from Water % Recovery min B A CN Analysis Conditions: Sorbent: strata-x 60 mg/3 ml Part No.: 8B-S100-UBJ Condition: 2 ml dichloromethane Equilibrate: 2 ml acetonitrile; 2 ml water Load: 2 ml spiked tap water Wash 1: 2 ml water; Dry for 2 min Elute: 2 ml of acetonitrile/dichloromethane (1:1) add 20 µg/ml of Linuron (external standard, E.S) Evaporate: under nitrogen and reconstitute with 200 µl water HPLC Column: Luna 5 µm C18(2) 150 x 4.6 mm Part No.: 00F-4252-E0 Sample: 50 µl of reconstituted water extract Mobile Phase: A: water; B: acetonitrile Gradient: A/B (85:15) hold for 2 min to A/B (42:58) in 13 min hold for 2 min Flow Rate: 2 ml/min Temperature: 25 C Detector: 214 nm Peaks: 1. Fenuron (0.5 µg/ml) 4. Diuron (0.5 µg/ml) 2. Monuron (0.5 µg/ml) 5. Siduron (0.5 µg/ml) 3. Forchlorfenuron (0.5 µg/ml) 6. Linuron (E.S.) Chromatogram of Extracts: A) Blank B) Spiked Sample *Method can be scaled up for 1-2 L sample volumes.

23 strata- X for Reversed Phase Extraction of Phenols from Water environmental % Recovery TN Analysis Conditions: Sorbent: strata-x 500 mg/6 ml Part No.: 8B-S100-HCH Condition: 5 ml methanol Equilibrate: 5 ml water Load: 500 ml of sample (loaded in a continuous fashion) Elute: 5 ml acetone, followed by 5 ml methylene chloride Drying: extracts were dried using anhydrous sodium sulfate concentrated to 0.5 ml GC Column: Zebron ZB-5 Dimension: 30 m x 0.25 mm x 0.25 µm Part No.: 7HG-G Injection: 1 µl at 250 C in 11:1 split ratio Carrier Gas: Helium Oven Program: 40 C to 325 C at 9 C/min Detector: MS in selective ion mode Peaks: 1. Phenol 5. 2,4,6-Trichlorophenol 2. 2,4,6-Tribromophenol 6. 2-Nitrophenol 3. 2-Flurophenol 7. 2,4-Dinitrophenol 4. 2-Chlorophenol 8. o-cresol

24 24 Ordering Information strata -X, strata -X Material Characteristics Pore size 85 Å Particle Size 33 μm Particle shape Spherical Traditional Tubes ml (100/box) ml (50/box) ml (30/box) Phase 0 mg 60 mg 200 mg 500 mg 100 mg 200 mg 500 mg strata-x 8B-S100-TAK 8B-S100-UBJ 8B-S100-FBJ 8B-S100-HBJ 8B-S100-ECH 8B-S100-FCH 8B-S100-HCH strata-x-c 8B-S029-TAK 8B-S029-UBJ 8B-S029-FBJ 8B-S029-HBJ 8B-S029-ECH 8B-S029-FCH 8B-S029-HCH strata-x-cw 8B-S035-TAK 8B-S035-UBJ 8B-S035-FBJ 8B-S035-HBJ 8B-S035-ECH 8B-S035-FCH 8B-S035-HCH strata-x-aw 8B-S038-TAK 8B-S038-UBJ 8B-S038-FBJ 8B-S038-HBJ 8B-S038-ECH 8B-S038-FCH 8B-S038-HCH strata-x-cw for 1 g / 6 ml is available. Please call Phenomenex for more information.

25 -C, strata -X-CW, & strata -X-AW 25 High-Throughput 96-Well Plates (2/box) Phase mg 0 mg 0 mg 0 mg strata-x 8E-S100-NGB 8E-S100-AGB 8E-S100-TGB 8E-S100-UGB strata-x-c 8E-S029-AGB 8E-S029-TGB 8E-S029-UGB strata-x-cw 8E-S035-AGB 8E-S035-TGB 8E-S035-UGB strata-x-aw 8E-S038-AGB 8E-S038-TGB 8E-S038-UGB Flash & Large Scale Cleanup Giga Tubes ml (20/box) 0 ml (20/box) Phase 00 mg 00 mg g g strata-x 8B-S100-HDG 8B-S100-JDG 8B-S100-JEG strata-x-c 8B-S029-HDG 8B-S029-JDG strata-x-cw 8B-S035-HDG 8B-S035-JDG 8B-S035-JEG strata-x-aw 8B-S038-HDG 8B-S038-JDG Rapid Screening On-Line Extraction Part No. Description unit/box Price 00M-S033-B0-CB strata-x on-line extraction cartridge, 20 x 2.0 mm ea 00M-S036-B0-CB strata-x-cw on-line extraction cartridge, 20 x 2.0 mm ea CH Cartridge holder, 20 mm ea

26 26 INTRODUCING strata -XL and strata -XL-C Large particle polymeric sorbents for viscous samples and gravity flow applications Phase 2 g/12 ml (20/box) 2 g/20 ml (20/box) Contact Phenomenex for 96-well plate ordering information. 5 g/ 20 ml (20/box) Sample Preparation Method Development Software Instantly develops analyte specific SPE methods on strata-x sorbents Generates methods for traditional and high-through put screening Provides fast, accurate and simple SPE method development Material Characteristics Pore size Particle Size Particle Shape 5 g/60 ml (16/box) 300 Å 100 μm Spherical 10 g/ 60 ml (16/box) strata-xl 8B-S043-KDG 8B-S043-KEG 8B-S043-LEG 8B-S043-LFF 8B-S043-MFF strata-xl-c 8B-S044-KDG 8B-S044-KEG 8B-S044-LEG 8B-S044-LFF 8B-S044-MFF Part No. Description unit/box Price AA Software: Strata Method Development ea Full Program

27 ordering Information SPE Accessories Well Plate Accessories Part No. Description unit Price Manifold AH Well Plate Manifold, Acrylic ea Sealing Mats AH Pierceable Sealing Mats, 96 Square Well 50/pk AH Sealing Tape Pad 10/pk Collection Plates AH ml/well 96 Square Well Collection Plate 50/pk AH ml/well 96 Square Well Collection Plate 50/pk AH ml/well 96 Square Well Collection Plate 50/pk AH Solvent Waste Reservoir Tray 25/pk Vacuum Manifolds* Part No. Description unit Price 10-Position Vacuum Manifold AH Tall-Boy Vacuum Manifold ea Complete Assembly for Giga Tubes 12-Position Vacuum Manifold AH Complete Assembly for 1, 3, 6 ml Tubes ea 24-Position Vacuum Manifold AH Complete Assembly for 1, 3, 6 ml Tubes ea * Replacement parts available contact your local Phenomenex technical consultant or distributor. If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE products for FREE!

28 28 Polymeric Sorbents for Sample Preparation Better Reproductibility Cleaner Extracts Faster Method Development Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department by telephone, fax or tel.: fax: tel.: fax: Australia phenomenex.com.au Ireland phenomenex.com Austria phenomenex.com Italy phenomenex.com Canada (800) (310) phenomenex.com New Zealand phenomenex.co.nz Denmark phenomenex.com Puerto Rico (800) 541-HPLC (310) phenomenex.com France phenomenex.com United Kingdom phenomenex.com Germany phenomenex.com USA (310) (310) phenomenex.com Luna and Gemini are registered trademarks of Phenomenex, Inc. Zebron, SecurityGuard, Synergi, Strata, strata-x, strata X-C, strata X-CW, strata X-AW, strata-xl, and strata-xl-c are trademarks of Phenomenex. Oasis is a registered trademark of Waters Corp. Phenomenex is not affiliated with Waters Corp. SPE applications shown may not be representative of all SPE applications Phenomenex, Inc. All rights reserved 4855_I