7200 Series Q-TOF for GC/MS. A new analytical tool for solving complex analytical problems

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1 7200 Series Q-TOF for GC/MS A new analytical tool for solving complex analytical problems

2 What Will GC/Q-TOF Do For You? TOF mode (full spectrum acquisition) High resolution full scan spectra Accurate mass measurements Fast scanning of full spectra Q-TOF mode (MS/MS) Full spectrum of Product Ions (SIM-TOF) With high resolution and accurate mass Selectivity beyond any triple quad MRM method Confirm TOF spectrum without interferences High sensitivity structural elucidation tool Ideal tool for solving complex analytical problems

3 What is it? = 7200 GC/Q-TOF Triple Quadrupole MS + = Time of Flight MS Quadrupole Time of Flight MS

4 7200 Q-TOF Comp NEW Removable Ion Source 7000B TQ NEW Optics 6500 Q-TOF Two 300 L/s Turbos

5 Removable Ion Source (RIS) Automated Gate Valve RIS Automated Retractable Transfer Line

6 30 Minute Source Swap with RIS Manual Steps Automated Steps Install extraction tool Rinse the chamber with nitrogen Pump out chamber Open gate valve/retract transfer line Withdraw source into chamber Close gate valve/re-engage transfer line Vent chamber and cool ion source Open chamber Replace/maintain source components Close chamber Rinse chamber & new source with N 2 Pump out chamber Open gate valve/retract transfer line Insert source into the manifold Close gate valve/re-engage transfer line Pressurize the chamber Remove extraction tool Load appropriate tune (heat source) New Clean Ion Source Original Dirty Ion Source 1pg OFN

7 Removable Ion Source (RIS) RIS Standard on Q-TOF Allows fast EI/CI source swapping without venting Allows swap of complete ion source, including filaments, in ~30 minutes without venting

8 Response Response Large dynamic range detection system ADC is important for chromatographic peaks Time-to-Digital Converter Analog-to-Digital Converter Ions Ions TDC has limited linear range and is quantitative over a small concentration range ADC has greater linear range and is quantitation over a larger concentration range

9 New Q-TOF System... Yet Totally Proven 4GHz ADC electronics enable a high sampling rate (32 Gbit/s) which improves the resolution, mass accuracy, and sensitivity for low-abundance samples. Hot, quartz monolithic quadrupole analyzer and collision cell identical to the 7000 Quadrupole MS/MS Dual-stage ion mirror improves second-order time focusing for high mass resolution. Proprietary INVAR flight tube sealed in a vacuum-insulated shell eliminates thermal mass drift due to temperature changes to maintain excellent mass accuracy, 24/7. Added length improves mass resolution. Analog-to-digital (ADC) Detector: Unlike time-to-digital (TDC) detectors which record single ion events, ADC detection records multiple ion events, allowing very accurate mass assignments over a wide mass range and dynamic range of concentrations. New Removable Ion Source includes repeller, ion volume, extraction lens and dual filaments Two 300L/s t urbos pump the focusing optics and flight tube Split-flow turbo differentially pumps the ion source and quadrupole analyzer compartments Hexapole collision cell accelerates ion through the cell to enable faster generation of high-quality MS/MS spectra without cross-talk

10 Spectral presentation (Tune File) Most users think in centroid, but the MS operates in profile Profile View Centroid View

11 Resolving Power R = 614/0.68 = 903 Mz=614 Single Quad or Ion trap Resolving Power, R=Mass/PW Pw=0.68 TOF

12 OFN Results 1pg OFN Theoretical Mass = error = -0.5 ppm Resolving Power = FWHM u 0.25 u

13 High Res TOF to Eliminate Isobaric Interferences Appears as Single 240 u Mass Peak for Any Unit Mass Resolution MS Δ m/z = Flurenol methyl ester m/z = Mass error = 1.7 ppm Dimetilan m/z = Mass error = 0.4 ppm ~13,500 resolution FWHM No Internal Reference Mass (IRM) corrections applied 13

14 Mass Accuracy Δmz = 0.1/614 = 160 ppm scan Mz=614 SQ IT TQ Mass Accuracy Δmz=dm/mz*1E6, ppm Pw=0.68 Δmz < 1ppm TOF

15 # Possible Chemical Formulas Many possible formulas with an MSD or IT But only a few with TOF Possible Number of Chemical Formulas at m/z 272 Octafluoronaphthalene (CAS ) C 10 F 8 = mass uncertainty, ppm mass uncertainty ppm # of Possible Formulas amu Formulas made of: C,H,N,O,F, & Cl Accurate mass reduces risk of investing effort on the wrong molecule

16 Internal Reference Mass (IRM) Engineering goal for the Agilent 7200: easy and reliable ~2-5ppm mass accuracy under all conditions Design results: Proprietary INVAR flight tube Sealed in a vacuum-insulated shell eliminates thermal mass drift due to temperature Internal Reference Mass (IRM) delivery system for on the fly mass axis correction IRM is often not needed since it may be an interference with analyte ions Locks the mass axis to known background ions Requires reliable IRM calibration signals with and without matrix

17 Example of IRM Correction Mass accuracy of 2-formyl thiophene and 2-acetyl thiazole in spiked Whiskey 1 pg on col DG 2 pg on col DG 5 pg on col DG 10 pg on col DG 20 pg on col DG 50 pg on col DG 100 pg on col DG 200 pg on col DG 500 pg on col DG 1000 pg on col DG uncorrected IRM corrected calc mass obs mass ppm Error obs mass ppm Error 2-formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole formyl thiophen acetyl-thiazole Average ABS Error Standard Deviation of Error Excellent: < 5 ppm Excellent: < 5 ppm Good: < 10 ppm Good: < 10 ppm Average mass error < 2 ppm over X1000 concentration range

18 TOF Accurate Mass to Eliminate Matrix Interferants Okra QuEChERS Extract Matrix interferant ion (b-tocopherol) Da Analyte Indoxacarb ion (100pg) Da (fragment ion) +/-0.5 amu (~ 300ppm) b-tocopherol Indoxacarb 5 ppm Tocopherol interference eliminated in MS mode Indoxacarb If even more selectivity is needed, option of MS/MS MS/MS with high resolution and accurate mass!

19 Q-TOF MS/MS for Structural Elucidation C16H14O4 (Rings + Double Bonds = 10) (M H) Candidate structures H Fragment Sequence from Multiple MS/MS Experiments m/z (experimental) Formula Error (ppm) Score C 16 H 13 O C 6 H C 10 H 9 O CH=CH C 6 H C 8 H 7 O N/A CH 2 =CH C 6 H 5 CO C 8 H 6 O N/A C 7 H 6 O CO C 6 H 6 O N/A CH C 5 H 3 O Candidate structure #4 fits the fragmentation information the best 19

20 What About TOF SPEED? TOF always collects full mass range Q-TOF always display full product ion spectrum Spectral Rate: Typical max rate: spectra/sec (Hz) to disk Usable rate is limited by signal level (ion count) New analysis opportunities for GC/MS: Fast GC and Ultra high resolution GC: ~ 20-40Hz

21 High Data Rate = Better Deconvolution Time Slow data rate will not pick each peak apex Time Fast data rate will allow deconvolution of closely eluting peaks

22 High Data Rate = Better Deconvolution The 7200 Q-TOF in TOF mode will have: Faster Data Rates Maintain Excellent S/N New MassHunter Deconvolution Time Slow data rate will not pick each peak apex Time Fast data rate will allow deconvolution of closely eluting peaks

23 7200 Performance Specifications Resolving Power: >12.5K at m/z K to 15K typical Mass Accuracy: <5 ppm at m/z 272 <2 ppm typical MS Sensitivity >2500:1 at m/z=272 MS/MS Sensitivity is between SRM and Product Ion scan of a Tandem Quad MS Dynamic Range: > 3 orders of magnitude 4 orders with Dual Gain TOF Mass range: Da MS/MS Mass range: Da ( Da FWHM) Spectral Rate: 1-50 Spectra/sec

24 Batch-at-a-Glance (also Compound-at-a-Glance) Powerful Navigation

25 Batch-at-a-Glance Batch Table: Single Compound/Multiple Compounds Single Compound, Qualifier, ISTD, ISTD Qualifier Multiple Compounds

26 Outlier Detection Over 20 criteria Limits in method Flagging & Filtering Outlier Report

27 MassHunter Winning Benefits Summary Less time spent reviewing data means faster sample throughput Batch- & Compound-at-a-glance Automated file open actions Parameter-less integration designed for MS data Flexible reporting capabilities means your data, your way Unification of your Agilent MS software MassHunter Data Processing used across all MS product lines: GC/MS, LC/MS, and ICP-MS MassHunter: increased productivity tool for your MS workflow

28 7200 Series Q-TOF for GC/MS How is this new capability used - application results Nobuo Ochiai, Kikuo Sasamoto GERSTEL KK, Japan Anthony Gravell Environment Agency, England and Wales Hans Mol RIKILT Institute of Food Safety, Wageningen, Netherlands Tom Doherty, Ryo Ogasawara, Viorica Lopez-Avila, Stephan Baumann Agilent Technologies

29 Sulfur-Containing Compounds in Beverages

30 Compounds Affecting Taste and Flavor 2-formyl thiophene and 2-acetyl thiazole are common contaminants Low sensory threshold and can have negative effect on product flavor or aroma Often requires sophisticated extraction/enrichment procedures and/or powerful 2D GC techniques for separation from matrix for quantitation

31 TIC and EICs of Coffee Extract 2-formyl thiophene m/z ± 100 ppm 2-acetyl thiazole m/z ± 100 ppm

32 Calibration in Matrix STD addition calibration curve for 2-acetyl thiazole in spiked coffee (STD amount: pg) Average mass error = 1.1 ppm (max 1.6) over entire concentration range m/z Mass window: ±20 ppm

33 Environmental: Quantitative Analysis of SPMD Extracts in River SPMD: semi-permeable membrane device

34 Range of Compounds Polyaromatic Hydrocarbons (PAHs) Polychlorinated Biphenyls (PCBs) Organochlorine Pesticides (OCPs) Polybrominated Diphenyl Ethers (PBDEs) Polycyclic Musk Fragrances (PCMs)

35 Mass Accuracy (Standards) Standard solution 100 ppb Molecular formula Exact mass Mass error (ppm) Mass error (ppm) fluorene C13H hexachlorobutadiene C4Cl hexachlorobenzene C6Cl dieldrin C12H8Cl6O BZ # 52 (2,2',5,5' -tetrachlorobiphenyl) C12H6Cl BDE-47 C12H6Br4O DPMI (Cashmeran) C14H22O HHCB (Galaxolide) C18H26O Average uncorrected corrected Standard solution 0.5 ppb Molecular formula Exact mass Mass error (ppm) Mass error (ppm) fluorene C13H hexachlorobutadiene C4Cl hexachlorobenzene C6Cl dieldrin C12H8Cl6O BZ # 52 (2,2',5,5' -tetrachlorobiphenyl) C12H6Cl BDE-47_1 C12H6Br4O DPMI (Cashmeran) C14H22O HHCB (Galaxolide) C18H26O Average uncorrected corrected

36 Mass Accuracy in Matrix Compound Matrix Avg (n = 2), ppb Exact mass Measured mass ppm diff acenaphthylene PAH_extract from SRM 1941b 19.2 ± hexachlorobenzene BZ # 52 BDE- 47 PAH_extract from SRM 1941b 2.98 ± PAH_extract from SRM 1941b 0.81 ± PAH_extract from SRM 1941b 1.10 ± Cashmeran Waste water SPMD drain 573 ±

37 Accurate Mass is Necessary to Eliminate Matrix Interferences SPMD river, Dual Gain TIC EIC amu Hexachlorobenzene EIC ppm

38 EIC Examples: Waste Water SPMD Drain Phenanthrene Anthracene Pyrene Extraction window: +/- 20 ppm Acenaphthene Fluoranthene Fluorene Benz[a]antracene Benzo[a]pyrene

39 Why Add Q to TOF to Make Q-TOF? Sometimes the combined power of GC Resolution (Agilent) + MS Resolving Power (Agilent)

40 Additional Applications Performed with GC/Q-TOF Petrochemicals Analysis of biomarkers in crude oil Analysis of oxidized fuel Forensics Synthetic cannabinoids in herbal blends Environmental Fluorotelomer alcohols Chlorinated paraffins Food testing Olive oil adulteration Photodegradation of beer Industrial Identification of impurities Doping

41 Where is MS/MS Used? To improve selectivity When high res TOF still has interferences Structural elucidation from product Ion spectra using high resolution and accurate mass Confirmation of identified unknown

42 Why Add Q to TOF to Make Q-TOF? Sometimes the combined power of GC Resolution (Agilent) + MS Resolving Power (Agilent) Is not enough based upon: Small ΔMass Defect (Nature) + Intense Matrix Ions (Sample) Add Q+CID to TOF solution High resolution MS/MS can solve some of these problems

43 A Few Conclusions The world about us is always changing and new problems are always emerging New problems often require new tools to find a solution The GC Q-TOF offers capability to solve new problems in new ways Higher resolution (HR), better mass accuracy (MA), and faster scan speed always improves analytical results Add HR and AM to MS/MS product ions and structure elucidation is possible Agilent offers the largest range of GC/MS tools SQ, IT, TQ, & Q-TOF

44 There are known knowns; there are things we know we know. We also know there are known unknowns; that is to say we know there are some things we do not know. But there are also unknown unknowns the ones we don't know we don't know. Former United States Secretary of Defense Donald Rumsfeld February 12, 2002 at a press briefing

45 Additional Slides

46 Pesticides in Food Matrices Target Confirmation and Selectivity of MS/MS

47 aldrin isodrin dieldrin endrin Mass Accuracy of Low Concentrations Analytes in Heavy Matrix (TOF mode) EIC (Leek extract) drins 10 pg ±0.5 Da drins 10 pg ±20 ppm

48 Mass Accuracy of Low Concentrations Analytes in Heavy Matrix (TOF mode) Endrin -2.7 ppm

49 MS/MS Experiments for Target Confirmation product ion spectrum of endrin m/z 263 C 7 H 2 Cl 4 [ 37 Cl] CE 20 ev C 7 H 2 Cl 2 [ 37 Cl] C 7 H 2 Cl 3 [ 37 Cl] 1.85 ppm 1.09 ppm C 5 HCl 3 [ 37 Cl] 6.86 ppm 6.1 ppm CE 35 ev 263 Cl Cl Cl Cl 281 O 245 Cl Cl MS/MS generates multiple ion fragments that can be used for confirmation

50 MS/MS Experiments for Selectivity Enhancement drins 10 pg ±20 ppm

51 MS/MS Experiments for Selectivity Enhancement interference m/z 263 endrin TIC of Product Ions from fragmentation of m/z 263 CE 35 ev drins 10 pg ±20 ppm

52 MS/MS Experiments for Selectivity Enhancement interference m/z 263 endrin TIC of Product Ions from fragmentation of m/z 263 CE 35 ev drins 10 pg ±20 ppm endosulfanalpha, chlordane dieldrin endrin EIC of product ion 263>

53 Targeted and Untargeted Metabolomics: Dissecting Drug Mechanism by Metabolic Profiling of Yeast Sterols

54 Metabolic Profiling of Yeast Sterols Changes in sterol metabolism were evaluated following the treatment with known inhibitors (Fluconazole and Terbinafine) as well as new chemical entity Mass Profiler Professional (MPP) was used for statistical evaluation of the data after deconvolution (data filtering, baseline correction, statistical significance, finding unique compounds in trace levels and visualization)

55 Terbinafine Treatment Terbinafine inhibits the ERG1 gene product, which is a squalene epoxidase. As we expected squalene abundance in terbinafine treated samples increased significantly. mass extraction window: +/-20 ppm squalene Accurate mass data allow narrow mass extraction window helping eliminate matrix interferences.

56 Fluconazole Treatment Fluconazole inhibits the ERG11 gene product, a 14α-demethylase that prevents the conversion of lanosterol to its subsequent intermediate. Downsteam enzymes are not very selective and accumulation of several 14α-methyl sterols is observed Compounds accumulated in fluconazole-treated yeast Component Compound Formula Derivatized MI 25.0 Squalene C 30 H Lanosterol C 30 H 50 O , or Methyl 3-keto-4-methylzymosterol C 29 H 46 O Methyl 4-α-methyl zymosterol C 29 H 48 O Methyl zymosterol C 28 H 46 O , or Methyl fecosterol C 29 H 48 O , or Methyl episterol C 29 H 48 O Compounds other than lanosterol and squalene were tentatively identified based on the accurate mass information

57 Treatment with new chemical entity: Proposed step of ergosterol biosynthesis affected by Accurate mass information and excellent sensitivity in scan mode and dynamic range help to identify and quantify all intermediates of interest in one simple experiment!

58 Natural Product Structure Elucidation Aided by MS/MS Experiments

59 MS/MS for Structure Elucidation Start with full scan EI spectrum Use accurate mass to estimate molecular formula Perform CID experiments on molecular ion and selected fragment ions to confirm losses Use information from sequence of losses to estimate reconstructed molecular structure Precursor-product ion relationship is documented and ion molecular formula confirmed by accurate mass Requires multiple analyses and much more sensitive than NMR Will not replace NMR, but will complement nicely

60 The Problem Confirm Most Likely Structure Kava Extract - Compound B, C 16 H 14 O 4 (Rings + Double Bonds = 10) EI Full Scan (M H) Candidate structures

61 The Problem Confirm Most Likely Structure Kava Extract - Compound B, C 16 H 14 O 4 (Rings + Double Bonds = 10) EI Full Scan (M H) Candidate structures H MS/MS experimental measurements m/z (experimental) Formula Error (ppm) Score C 16 H 13 O C 6 H 5 CH=CH C 6 H 5 CH 2 =CH C 6 H 5 CO CO CH C 10 H 9 O C 8 H 7 O N/A C 8 H 6 O N/A C 7 H 6 O C 6 H 6 O N/A C 5 H 3 O

62 The Problem Confirm Most Likely Structure Kava Extract - Compound B, C 16 H 14 O 4 (Rings + Double Bonds = 10) EI Full Scan (M H) Candidate structures X H C 6 H 5 CH=CH C 6 H 5 MS/MS experimental measurements m/z (experimental) Formula Error (ppm) Score C 16 H 13 O C 10 H 9 O C 8 H 7 O N/A X CH 2 =CH C 6 H 5 CO CO CH C 8 H 6 O N/A C 7 H 6 O C 6 H 6 O N/A C 5 H 3 O

63 The Problem Confirm Most Likely Structure Kava Extract - Compound B, C 16 H 14 O 4 (Rings + Double Bonds = 10) EI Full Scan (M H) Candidate structures H MS/MS experimental measurements m/z (experimental) Formula Error (ppm) Score C 16 H 13 O C 6 H 5 CH=CH C 6 H 5 CH 2 =CH C 6 H 5 CO CO CH C 10 H 9 O C 8 H 7 O N/A C 8 H 6 O N/A C 7 H 6 O C 6 H 6 O N/A C 5 H 3 O

64 Problem confirm most likely structure? c Mass at 138 is consistent with: loss of COCH=CH-C 6 H 5 ( ) from or loss of C 2 H 4 CH=CH-C 6 H 5 ( ) from However, measured value of = is consistent only with COCH=CH- C 6 H 5.

65 Problem confirm most likely structure EI Full Scan (M H) Candidate structures H MS/MS experimental measurements m/z (experimental) Formula Error (ppm) Score C 16 H 13 O C 6 H C 10 H 9 O CH=CH C 6 H C 8 H 7 O N/A CH 2 =CH C 6 H 5 CO C 8 H 6 O N/A C 7 H 6 O CO C 6 H 6 O N/A CH C 5 H 3 O For the 5 candidate structures, only one fit the losses identified by CID experiments on multiple precursor ions