Nitric Oxide and Lipid Peroxidation
|
|
- Octavia Baldwin
- 5 years ago
- Views:
Transcription
1 Nitric Oxide and Lipid Peroxidation Valerie B. O Donnell a, Neil Hogg b and Victor M. Darley Usmar c a Dept of Med. Biochem. University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK. Ph: Fax: o-donnellvb@cardiff.ac.uk b Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI USA. c Department of Pathology, University of Alabama at Birmingham, AL USA NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 1
2 Nitric Oxide is a Free Radical N=O NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 2
3 Nitric oxide is not particularly oxidizing. Nitric oxide is (infinitely) stable*. Nitric oxide does not rapidly react with organic molecules. *In the absence of dioxygen. NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 3
4 The Nitric Oxide Radical and some of its stable end-products N=O nitric oxide - O-N=O nitrite RO-N=O alkyl nitrite RS-N=O nitrosothiol NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 4
5 Reactions of NO with Other Free Radicals Oxidation of NO to nitrite: 4 NO+ O=O +2H 2 O 4HNO 2 Nitric oxide reaction with organic peroxyl radicals: NO+ ROO ROONO?? NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 5
6 Lipid Peroxidation LOO + LH + O 2 LOO + LOOH LOO is the central and rate-limiting species of lipid peroxidation NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 6
7 LOOH Non-enzymatic lipid oxidation Metal or enzyme AOO O 2 LOO LH L LOOH O 2 LOO LH L LOOH O 2 LOO LH GPx GPx Initiation LOH Propagation LOH Propagation NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 7
8 Lipid peroxidation products in an atherosclerotic lesion are both enzymatically and nonenzymatically produced. Foam cell 15-LOX Necrotic core lipid hydroxides, hydroperoxides, aldehydes, epoxides, ketones, ceroid, isoprostanes Fibrous cap NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 8
9 Location of lipid oxidation enzymes in the vasculature LOX: lipoxygenase PGHS: prostaglandin H synthase Smooth muscle: 12-LOX Endothelium PGHS-1 Monocyte: PGHS-1,2 5, 15-LOX Platelet: PGHS-1 12-LOX Neutrophil: PGHS-1,2 5,12-LOX NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 9
10 Lipid oxidation enzymes are upregulated during vascular disease. * Inhibition of 12-LOX restores blood pressure in Angiotensin- II dependent hypertensive rats. * Increased products of PGHS in hypertensive rats, and PGHS inhibition restores blood pressure in humans and rats. * Smooth muscle 12-LOX in hypertension * Platelet 12-LOX in hypertension * Foam cell/macrophage 15-LOX in atherosclerosis. NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 10
11 Nitric oxide reaction with lipid peroxyl radicals LOO NO LOONO H 2 O [LO NO 2 ] LOOH + NO 2 - LO NO 2 NO [L(O) NO 2 ] NO LONO NO - 2 L(O)NO 2 NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 11
12 Reactions between NO-derived reactive nitrogen species and unsaturated lipid NO LOONO L(O)NO 2 O 2 O 2 - NO 2 ONOO - NO HONO LH O L 2 LOO e -, H + LOOH NO 2, ONOO - LNO 2 NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 12
13 Nitric oxide inhibition of lipid oxidation coincides with fast rates of NO uptake during ABAP-dependent linoleate oxidation. (A) (B) Oxygen (µm) 100 ABAP 0.2 NO (µm) [ NO] = 0.9 µm added at vertical lines to 7.3 mm linoleate in 0.5% cholate, 11 mm ABAP Time/s NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 13
14 Mechanism of Inhibition of LDL Oxidation by NO LOO + NO LOONO LO + NO 2 LONO 2 LOOH OLOO + NO OLOONO OLONO 2 Two NO consumed for each LOO 4 NO + 2LOO + H 2 O 2HNO 2 + LONO 2 + LONO NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 14
15 Cholesteryl Ester Triglyceride Phospholipid Apolipoprotein B Oxidant LH NO 2 O 2 O 2 L NO 2 LH LOO NO LNO 2 2 L α-tocopherol LOOH NO L(O)NO 2 Cholesterol NO NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 15
16 500 NO and LDL Oxidation: The effect of HPODE Lag time of LDL (125 µg/ml) oxidation by Cu(II) (20 µm) with and without HPODE (5 µm) length of lag time (min) A SNN SNN + HpODE SNN (µm) B BHT BHT + HpODE BHT (µm) NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 16
17 Comparison of nitric oxide and tocopherol inhibition of linoleate oxidation. 300 ABAP α -tocopherol (1 µm) [Oxygen]/µM T inh NO (1 µm) T inh Time/s Oxidation of linoleate (7.3 mm) by ABAP (11 mm) NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 17
18 Nitric oxide is consumed by rabbit 15-lipoxygenase during oxidation of linoleate. 2 LOX LOX LOX NO (µm) 1 Add figure caption 0 (i) (ii) (iii) Time (secs) NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 18
19 Site of lipoxygenase inhibition by NO E-Fe 2+ LOOH E-Fe 3+ NO E-Fe 3+ LOO E-Fe 2+ L O 2 E-Fe 2+ LOO NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 19
20 Activation of LOX inhibits activation of sgc by NO NO Lox 100 sgc cgmp cgmp (% control) 50 ** 0 control AA, 5 µm LOX AA, 5 µm, LOX NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 20
21 Further Reading: Darley-Usmar, VM et al., (1992) Free Radic. Res. Commun. 17, 9-20 Graham, A et al., (1993) FEBS Lett. 330, Rubbo, H et al., (1994) J. Biol. Chem. 265, Hogg, N et al., (1995) J. Lipid Res. 36, Struck, N et al., (1995) FEBS Lett 361, O Donnell, VB et al., (1997) Biochemistry 36, O Donnell, VB et al., (1999) Chem. Res. Toxicol. 12, O Donnell, VB et al., (1999) J. Biol. Chem. 274, Hogg, N & Kalyanaraman, B (1999) Biochim. Biophys. Acta. 1411, Rubbo, H et al., (2000) J. Biol. Chem. 275, O Donnell, VB & Freeman, BA (2001) Circ. Res. 88, NO & LPO Society For Free Radical Biology and Medicine O Donnell et al. 21
Enzymatic oxidation of lipids: mechanisms and functions.
Enzymatic oxidation of lipids: mechanisms and functions. Valerie B. O Donnell, PhD. Cardiff University. Enzymatic lipid oxidation: involves an enzyme catalyst, and gives very specific stereo- and regiospecific
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationReactivity and Biological Functions of Oxidized Lipids
Reactivity and Biological Functions of Oxidized Lipids Matthew J. Picklo, Sr. Outline 1. Lipid Oxidation Mechanisms and Products 2. Biological Mechanisms 3. n-3 vs n-6 PUFA Oxidation 1. Fatty acids 2.
More informationOXIDIZED LIPIDS FORMED NON-ENZYMATICALLY BY REACTIVE OXYGEN SPECIES
JBC Papers in Press. Published on February 19, 2008 as Manuscript R800006200 The latest version is at http://www.jbc.org/cgi/doi/10.1074/jbc.r800006200 OXIDIZED LIPIDS FORMED NON-ENZYMATICALLY BY REACTIVE
More informationTypes of Free Radicals
Terminology Lipid Oxidation and Lipid peroxidation Free Radicals and Reactive oxygen species Fatty acyl group and methylene group Hydroperoxides and lipid peroxides Peroxyl radicals and alkoxyl radicals
More informationNitric Oxide Inhibits Iron Induced Lipid Peroxidation in HL-60 Cells 1
Archives of Biochemistry and Biophysics Vol. 370, No. 1, October 1, pp. 97 104, 1999 Article ID abbi.1999.1386, available online at http://www.idealibrary.com on Nitric Oxide Inhibits Iron Induced Lipid
More informationCholesterol Oxidation. Mechanisms and Signature Products
: Mechanisms and Signature Products Albert W. Girotti, Ph.D. Department of Biochemistry Medical College of Wisconsin Milwaukee, WI 53226, USA Tel: 414-456-8432 Fax: 414-456-6510 E-mail: agirotti@mcw.edu
More informationPreservation methods for fish oil
Preservation methods for fish oil Charlotte Jacobsen Professor and group leader Research group for Bioactives Analysis and Application chja@food.dtu.dk Agenda Intro to antioxidants Different examples on
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2003 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2003) offered
More informationReview. Interactions Between Nitric Oxide and Lipid Oxidation Pathways: Implications for Vascular Disease
Review This Review is part of a thematic series on Oxidant Signaling in Cardiovascular Cells, which includes the following articles: NAD(P)H Oxidase: Role in Cardiovascular Biology and Disease Oxidant
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationPart 1 Risk Factors and Atherosclerosis. LO1. Define the Different Forms of CVD
Week 3: Cardiovascular Disease Learning Outcomes: 1. Define the difference forms of CVD 2. Describe the various risk factors of CVD 3. Describe atherosclerosis and its stages 4. Describe the role of oxidation,
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2001 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2001) offered
More informationReactive Oxygen species ROS + Anti-oxidants. Dr. Naif Karadsheh
Reactive Oxygen species ROS + Anti-oxidants Dr. Naif Karadsheh Oxygen Toxicity & Free Radicals Biradical O 2 Radical O 2 Non-Radical Radical H 2 O 2 OH ROS O 2 Metabolism and Toxicity O 2 Consumption >90%
More informationLipid Oxidation and its Implications to Food Quality and Human Health. Dong Uk Ahn Animal Science Department Iowa State University
Lipid Oxidation and its Implications to Food Quality and Human Health Dong Uk Ahn Animal Science Department Iowa State University Introduction Process of Lipid Oxidation Free Radicals and Reactive Oxygen
More informationEicosapentaenoic Acid and Docosahexaenoic Acid: Are They Different?
Eicosapentaenoic Acid and Docosahexaenoic Acid: Are They Different? Trevor A Mori, Ph.D., Professor, School of Medicine and Pharmacology, Royal Perth Hospital Unit, University of Western Australia, Perth,
More informationRelationship between serum glutathione peroxidase-1activity with endothelial dysfunction level in patients with coronary artery diseases
Relationship between serum glutathione peroxidase-1activity with endothelial dysfunction level in patients with coronary artery diseases Introduction Reactive oxygen species (ROS),such as superoxide and
More informationGlossary For TheFatNurse s For All Ages Series Adipocytes, also known as lipocytes and fat cells, are the cells that primarily compose adipose tissue, specialized in storing energy as fat. Apolipoprotein
More informationWhat is an antioxidant? How do antioxidants work?
What is an antioxidant? How do antioxidants work? Garry R. Buettner and Freya Q. Schafer Free Radical and Radiation Biology Program and ESR Facility The University of Iowa Iowa City, IA 52242-1101 Tel:
More informationLipid/Lipoprotein Structure and Metabolism (Overview)
Lipid/Lipoprotein Structure and Metabolism (Overview) Philip Barter President, International Atherosclerosis Society Centre for Vascular Research University of New South Wales Sydney, Australia Disclosures
More information1Why lipids cannot be transported in blood alone? 2How we transport Fatty acids and steroid hormones?
1Why lipids cannot be transported in blood alone? 2How we transport Fatty acids and steroid hormones? 3How are dietary lipids transported? 4How lipids synthesized in the liver are transported? 5 Lipoprotien
More informationIron Chelates and Unwanted Biological Oxidations
The Virtual Free Radical School Iron Chelates and Unwanted Biological Oxidations Kevin D. Welch and Steven D. Aust Department of Chemistry and Biochemistry Biotechnology Center Utah State University Logan,
More informationClean Label Solutions to Controlling Lipid Oxidation
Clean Label Solutions to Controlling Lipid Oxidation Eric A Decker Department of Food Science University of Massachusetts, Amherst, USA March 27-28, 2018 Itasca, Illinois, USA Why is Oxidation Important?
More informationThe Role of Fats. Fat provides flavour and texture to foods. Fat contributes to making us feel satiated because
The Role of Fats Fat provides flavour and texture to foods. Fat contributes to making us feel satiated because Fats are more energy dense than carbohydrates or protein Fats take longer to digest Emulsion
More informationArteriosclerosis & Atherosclerosis
Arteriosclerosis & Atherosclerosis Arteriosclerosis = hardening of arteries = arterial wall thickening + loss of elasticity 3 types: -Arteriolosclerosis -Monckeberg medial sclerosis -Atherosclerosis Arteriosclerosis,
More informationCholesterol and Cholesterol Oxides on Coronary Heart Diseases
Cholesterol and Cholesterol Oxides on Coronary Heart Diseases Trends in egg consumption in U.S. and the reason A. Egg Consumption: - 403 in 1945-234 in 1993 (176 Table eggs, 58 Processed eggs) - 256 in
More informationGeneration of reactive oxygen and nitrogen species in pea cultivars under copper exposure
Volume 55(2):273-278, 2011 Acta Biologica Szegediensis http://www.sci.u-szeged.hu/abs ARTICLE Generation of reactive oxygen and nitrogen species in pea cultivars under copper exposure Nóra Lehotai, Andrea
More informationMetabolism of xenobiotics FM CHE 5-6
Metabolism of xenobiotics FM 3.5-10 CHE 5-6 Metabolism of xenobiotics Also called:! Biotransformation, or detoxification Primary metabolism secondary metabolism Goal: To make use of a compound or to facilitate
More informationWhat Is Oxidative Stress?
Oxidative Stress What Is Oxidative Stress? JMAJ 45(7): 271 276, 2002 Toshikazu YOSHIKAWA* and Yuji NAITO** Professor* and Associate Professor**, First Department of Medicine, Kyoto Prefectural University
More informationEVALUATION OF ANTIOXIDANT ACTIVITY OF NEW NUTRACEUTICALS: IN VITRO AND CELLULAR ASSAYS. Ana Denicola
EVALUATION OF ANTIOXIDANT ACTIVITY OF NEW NUTRACEUTICALS: IN VITRO AND CELLULAR ASSAYS Ana Denicola Lab. Fisicoquímica Biológica. Instituto Química Biológica. Facultad de Ciencias, UdelaR. Center for Biomedical
More informationHow to increase oxidative stability in fats and oils
How to increase oxidative stability in fats and oils 2015. 04. 27. Department of Food Science and Biotechnology, Sungkyunkwan University JaeHwan Lee 1 2 Sungkyunkwan University Founded in 1398 as the highest
More informationStatus of LDL Oxidation and antioxidant potential of LDL in Type II Diabetes Mellitus
Biomedical Research 2010; 21 (4): 416-418 Status of LDL Oxidation and antioxidant potential of LDL in Type II Singh N, Singh N, Singh S K, Singh A K, Bhargava V. Department of Biochemistry, G. R. Medical
More informationab Lipid Hydroperoxide (LPO) Assay Kit
ab133085 Lipid Hydroperoxide (LPO) Assay Kit Instructions for Use For the measurement of hydroperoxides directly utilizing the redox reactions with ferrous ions. This product is for research use only and
More informationLipid Peroxidation in vivo
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 949 Lipid Peroxidation in vivo Evaluation and Application of Methods for Measurement BY EVA SÖDERGREN ACTA UNIVERSITATIS UPSALIENSIS
More informationComplications of Diabetes mellitus. Dr Bill Young 16 March 2015
Complications of Diabetes mellitus Dr Bill Young 16 March 2015 Complications of diabetes Multi-organ involvement 2 The extent of diabetes complications At diagnosis as many as 50% of patients may have
More informationDetection of Lipid Peroxidation Products From Free Radical and Enzymatic Processes. Jason D. Morrow M.D. Vanderbilt University School of Medicine
Detection of Lipid Peroxidation Products From Free Radical and Enzymatic Processes Jason D. Morrow M.D. Vanderbilt University School of Medicine Question? Which one of the following is the most accurate
More informationLipid oxidation and related protein modifications in oil-in-water emulsions
Lipid oxidation and related protein modifications in oil-in-water emulsions C. Berton-Carabin, M.-H. Ropers, D. Guibert, V. Solé, C. Genot May 6 th, 214 15 th AOCS AnnualMeeting San Antonio, Texas Context
More informationPathophysiology of Lipid Disorders
Pathophysiology of Lipid Disorders Henry Ginsberg, M.D. Division of Preventive Medicine and Nutrition CHD in the United States CHD is the single largest killer of men and women 12 million have history
More informationCHAPTER 2. Detection of nitric oxide and reactive oxygen species
CHAPTER 31 Chapter Introduction Nitric oxide (NO) is one of the key players in the vasculature. It is involved in vessel dilation, inhibition of platelet and leukocyte adhesion, and inhibition of proliferation
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2003 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2003) offered
More informationLOOKING FOR LIPID PEROXIDATION IN VITRO AND IN VIVO: IS SEEING BELIEVING? Vanderbilt University School of Medicine Jason D.
LOOKING FOR LIPID PEROXIDATION IN VITRO AND IN VIVO: IS SEEING BELIEVING? Vanderbilt University School of Medicine Jason D. Morrow MD Which of the following assays of lipid peroxidation may be useful and
More informationRelation between Lipid Profile and Total Antioxidant Status among Normal Population
Relation between Lipid Profile and Total Antioxidant Status among Normal Population N. Gupta *, P. Khadka, S. Yadav Department of Biochemistry, Universal Science College, Pokhara University, Kathmandu,
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationROLE OF ANTIOXIDANTS IN FEED AND EFFECTS ON MEAT QUALITY
ROLE OF ANTIOXIDANTS IN FEED AND EFFECTS ON MEAT QUALITY Sergi Carné*, Anna Zaragoza Technical Department Industrial Técnica Pecuaria, S.A. (ITPSA) *scarne@itpsa.com Rancidity is associated with the breakdown
More informationNitric oxide, peroxynitrite and lipoxygenase in atherogenesis: mechanistic insights
Toxicology 208 (2005) 305 317 Nitric oxide, peroxynitrite and lipoxygenase in atherogenesis: mechanistic insights Homero Rubbo a,, Valerie O Donnell b a Departamento de Bioquímica and Center for Free Radical
More informationEndothelium. A typical endothelial cell is about 30mm long, Accounts for 1% or less of the arterial weight
Endothelium Discovered in 1845 A typical endothelial cell is about 30mm long, 10mm wide, and 0.2 3 mm thick Accounts for 1% or less of the arterial weight As recently as the late 1960s it was thought of
More informationDyslipidemia Endothelial dysfunction Free radicals Immunologic
ATHEROSCLEROSIS Hossein Mehrani Professor of Clinical Biochemistry Definition Atherosclerosis: Is a chronic inflammatory process characterized by plaque formation within the vessel wall of arteries and
More informationImportant reactions of lipids
Taif University College of Medicine Preparatory Year Students Medical chemistry (2) Part II (Lipids) week 4 lectures 1435-36 Important reactions of lipids Lectures outlines Definition and importance of
More informationATHEROSCLEROSIS زيد ثامر جابر. Zaid. Th. Jaber
ATHEROSCLEROSIS زيد ثامر جابر Zaid. Th. Jaber Objectives 1- Review the normal histological features of blood vessels walls. 2-define the atherosclerosis. 3- display the risk factors of atherosclerosis.
More informationCell signalling by oxidized lipids and the role of reactive oxygen species in the endothelium
Cellular Information Processing 1385 Cell signalling by oxidized lipids and the role of reactive oxygen species in the endothelium J.W. Zmijewski*, A. Landar*, N. Watanabe*, D.A. Dickinson, N. Noguchi*
More informationCritical Review. Reactivity of Peroxynitrite and Nitric Oxide with LDL
IUBMB Life, 57(6): 407 412, June 2005 Critical Review Reactivity of Peroxynitrite and Nitric Oxide with LDL Horacio Botti, Andre s Trostchansky, Carlos Batthya ny and Homero Rubbo Departamento de Bioquı
More informationHuman Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General
Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General For the detection and quantitation of human OxLDL in plasma, serum or other biological fluid samples Cat. No. KT-959 For Research Use Only.
More informationEffects of Oxidized Low Density Lipoprotein on Nitric Oxide Production in Macrophages
East Tennessee State University Digital Commons @ East Tennessee State University Electronic Theses and Dissertations December 1997 Effects of Oxidized Low Density Lipoprotein on Nitric Oxide Production
More informationTHE USE OF STABILIZED FRYING FAT IN THE PRODUCTION OF POTATO CHIPS
Journal homepage: www.fia.usv.ro/fiajournal Journal of Faculty of Food Engineering, Ştefan cel Mare University of Suceava, Romania Volume XV, Issue 3-2016, pag. 249-254 THE USE OF STABILIZED FRYING FAT
More informationOriginal Contribution
PII S0891-5849(01)00737-7 Free Radical Biology & Medicine, Vol. 31, No. 12, pp. 1570 1581, 2001 Copyright 2001 Elsevier Science Inc. Printed in the USA. All rights reserved 0891-5849/01/$ see front matter
More informationModule 13: Changes occurring in oils and fats during frying
Module 13: Changes occurring in oils and fats during frying Rajan Sharma and Bimlesh Mann Dairy Chemistry Division National Dairy Research Institute, Karnal rajansharma21@gmail.com Frying is a process
More informationAbout OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
More informationLipoproteins Metabolism Reference: Campbell Biochemistry and Lippincott s Biochemistry
Lipoproteins Metabolism Reference: Campbell Biochemistry and Lippincott s Biochemistry Learning Objectives 1. Define lipoproteins and explain the rationale of their formation in blood. 2. List different
More informationCo-oxidation of NADH and NADPH by a mammalian 15-lipoxygenase: inhibition of lipoxygenase activity at near-physiological NADH concentrations
Biochem. J. (1997) 327, 203 208 (Printed in Great Britain) 203 Co-oxidation of NADH and NADPH by a mammalian 15-lipoxygenase: inhibition of lipoxygenase activity at near-physiological NADH concentrations
More informationAN ACTIVE SHELTER AGAINST POLLUTION V.16
AN ACTIVE SHELTER AGAINST POLLUTION V.16 THE SKIN IS CONSTANTLY EXPOSED The skin is an interface and thus is constantly exposed to environmental agents: Sun radiation Smoke Air pollution Free radicals
More informationStatins: 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitors demonstrate anti-atherosclerotic character due to their antioxidant capacity
Author version: Appl. Biochem. Biotechnol., vol.163(2); 2011; 215-222 Statins: 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitors demonstrate anti-atherosclerotic character due to their antioxidant
More informationAlgorithmic Simulation and Mathematical Modeling in Studying the Kinetics of Iron (II)-Ascorbate-Dependent Lipid Peroxidation
Algorithmic Simulation and Mathematical Modeling in Studying the Kinetics of Iron (II)-Ascorbate-Dependent Lipid Peroxidation Anatole D. Ruslanov and Anton V. Bashylau, Members, IAENG Abstract We studied
More informationOxiSelect Human Oxidized LDL ELISA Kit (OxPL-LDL Quantitation)
Product Manual OxiSelect Human Oxidized LDL ELISA Kit (OxPL-LDL Quantitation) Catalog Number STA-358 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipoproteins are submicroscopic
More informationNitrolinoleate, a nitric oxide-derived mediator of cell function: Synthesis, characterization, and vasomotor activity
Nitrolinoleate, a nitric oxide-derived mediator of cell function: Synthesis, characterization, and vasomotor activity Dong Gun Lim*, Scott Sweeney*, Allison Bloodsworth*, C. Roger White, Phillip H. Chumley*,
More informationANTIOXIDANT ACTIVITY OF THE 1,7-DIARYLHEPTANOIDS AND THEIR METAL COMPLEXES
ANTIOXIDANT ACTIVITY OF THE 1,7-DIARYLHEPTANOIDS AND THEIR METAL COMPLEXES Malini.P.T Lanthanide complexes of curcuminoids Thesis. Department of Chemistry, University of Calicut, 2004 CHAPTER IV ANTIOXIDANT
More informationRole of apolipoprotein B-containing lipoproteins in the development of atherosclerosis Jan Borén MD, PhD
Role of apolipoprotein B-containing lipoproteins in the development of atherosclerosis Jan Borén MD, PhD Our laboratory focuses on the role of apolipoprotein (apo) B- containing lipoproteins in normal
More informationPCTH 400. Endothelial dysfunction and cardiovascular diseases. Blood vessel LAST LECTURE. Endothelium. High blood pressure
PCTH 400 LAST LECTURE Endothelial dysfunction and cardiovascular diseases. Classic Vascular pharmacology -chronic -systemic Local Vascular pharmacology -acute -targeted High blood pressure Blood pressure
More informationPHM142 Lecture 4: Platelets + Endothelial Cells
PHM142 Lecture 4: Platelets + Endothelial Cells 1 Hematopoiesis 2 Platelets Critical in clotting - activated by subendothelial matrix proteins (e.g. collagen, fibronectin, von Willebrand factor) and thrombin
More informationINFLAMMATION & REPAIR
INFLAMMATION & REPAIR Lecture 7 Chemical Mediators of Inflammation Winter 2013 Chelsea Martin Special thanks to Drs. Hanna and Forzan Course Outline i. Inflammation: Introduction and generalities (lecture
More informationMING TIEN*, BARBARA S. BERLETT, RODNEY L. LEVINE, P.BOON CHOCK, AND EARL R. STADTMAN MATERIALS AND METHODS
Proc. Natl. Acad. Sci. USA Vol. 96, pp. 7809 7814, July 1999 Biochemistry Peroxynitrite-mediated modification of proteins at physiological carbon dioxide concentration: ph dependence of carbonyl formation,
More informationSource Variation in Antioxidant Capacity of Cranberries from Eight U.S. Cultivars
33 Source Variation in Antioxidant Capacity of Cranberries from Eight U.S. Cultivars Peter J. Schaaf Faculty Sponsors: Margaret A. Maher and Ted Wilson, Departments of Biology/Microbiology ABSTRACT Antioxidants
More informationThe Effect of Vitamin E Supplementation on Lipid Peroxidation in Patients with Endothelial Dysfunction
The Effect of Vitamin E Supplementation on Lipid Peroxidation in Patients with Endothelial Dysfunction Corina Zorila 1,Liana Mos 1, Elena Magopat 2, Eva Mera 3 1.Western University V. Goldis Arad, 2.Clinical
More informationCatalysts of Lipid Oxidation
Catalysts of Lipid Oxidation Iron The most important nonenzymic catalyst for initiation of lipid peroxidation The most abundant transitional metal in biological systems Possibility of various oxidation
More informationImpact of Chemical and Physical Properties on the Ability of Antioxidants to Inhibit Lipid Oxidation in Foods
University of Massachusetts Amherst ScholarWorks@UMass Amherst Masters Theses 1911 - February 2014 2008 Impact of Chemical and Physical Properties on the Ability of Antioxidants to Inhibit Lipid Oxidation
More informationCholesterol is more susceptible to oxidation than linoleates in cultured cells under oxidative stress induced by selenium deficiency and free radicals
Cholesterol is more susceptible to oxidation than linoleates in cultured cells under oxidative stress induced by selenium deficiency and free radicals Yoshiro Saito *, Yasukazu Yoshida, Etsuo Niki Human
More informationLipid metabolism in familial hypercholesterolemia
Lipid metabolism in familial hypercholesterolemia Khalid Al-Rasadi, BSc, MD, FRCPC Head of Biochemistry Department, SQU Head of Lipid and LDL-Apheresis Unit, SQUH President of Oman society of Lipid & Atherosclerosis
More informationCHAPTER 28 LIPIDS SOLUTIONS TO REVIEW QUESTIONS
28 09/16/2013 17:44:40 Page 415 APTER 28 LIPIDS SLUTINS T REVIEW QUESTINS 1. The lipids, which are dissimilar substances, are arbitrarily classified as a group on the basis of their solubility in fat solvents
More informationOxiSelect Human Oxidized LDL ELISA Kit (MDA- LDL Quantitation)
Product Manual OxiSelect Human Oxidized LDL ELISA Kit (MDA- LDL Quantitation) Catalog Number STA- 369 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipoproteins are
More informationG.J.B.B., VOL.4 (3) 2015: ISSN LIPID OXIDATION IN BIOLOGICAL SYSTEMS: BIOCHEMICAL, BIOLOGICAL & BIOPHYSICAL ASPECTS
G.J.B.B., VOL.4 (3) 2015: 224-233 ISSN 2278 9103 LIPID OXIDATION IN BIOLOGICAL SYSTEMS: BIOCHEMICAL, BIOLOGICAL & BIOPHYSICAL ASPECTS Y. K. Lahir Department of Biophysics, University of Mumbai, Santa Cruz
More informationAshlee N. Higdon. (205)
Ashlee N. Higdon ashleehigdon@yahoo.com (205) 413-2200 Education PhD, Molecular and Cellular Pathology Program September 2011 University of Alabama at Birmingham Dissertation: Protein Modifications and
More informationEffects of Diet, Packaging and Irradiation on Protein Oxidation, Lipid Oxidation of Raw Broiler Thigh Meat
AS 659 ASL R26 203 Effects of Diet, Packaging and Irradiation on Protein Oxidation, Lipid Oxidation of Raw Broiler Thigh Meat Shan Xiao Iowa State University Wan Gang Zhang Iowa State University Eun Joo
More informationThe 10 th International & 15 th National Congress on Quality Improvement in Clinical Laboratories
The 10 th International & 15 th National Congress on Quality Improvement in Clinical Laboratories Cardiac biomarkers in atherosclerosis Najma Asadi MD-APCP Ross and Colleagues in 1973: Response to Injury
More informationLujain Hamdan. Faisal Nimri
20 Lujain Hamdan Faisal Nimri...... Sources of NADPH [ The pentose phosphate pathway is the primary source of the NADPH and is the only source in RBC.] Cytosolic conversion of oxaloacetate to pyruvate
More informationBiological Chemistry of Hydrogen Peroxide
Biological Chemistry of Hydrogen Peroxide Christine Winterbourn Department of Pathology University of Otago, Christchurch New Zealand Hydrogen Peroxide Intermediate in reduction of oxygen to water A major
More informationCHAPTER 28 LIPIDS SOLUTIONS TO REVIEW QUESTIONS
HAPTER 28 LIPIDS SLUTINS T REVIEW QUESTINS 1. The lipids, which are dissimilar substances, are arbitrarily classified as a group on the basis of their solubility in fat solvents and their insolubility
More informationNafith Abu Tarboush DDS, MSc, PhD
Nafith Abu Tarboush DDS, MSc, PhD natarboush@ju.edu.jo www.facebook.com/natarboush Lipids (cholesterol, cholesterol esters, phospholipids & triacylglycerols) combined with proteins (apolipoprotein) in
More informationOxiSelect Hydrogen Peroxide Assay Kit (Colorimetric)
Product Manual OxiSelect Hydrogen Peroxide Assay Kit (Colorimetric) Catalog Number STA-343 5 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Oxidative stress is a physiological
More informationTracking a Killer Molecule
Tracking a Killer Molecule Mercodia Oxidized LDL ELISA www.mercodia.com Mercodia Oxidized LDL ELISA products Product Catalog No Kit size Oxidized LDL ELISA 10-1143-01 96 wells Oxidized LDL competitive
More information8. OXIDATIVE STRESS IN DIABETICS
8. OXIDATIVE STRESS IN DIABETICS Prof. Victor Blaton, Ph.D. Department of Clinical Chemistry, Hospital AZ Sint-Jan AV, Brugge, Belgium 1.1. Introduction Diabetes mellitus is a major source of morbidity
More informationRetrotope, Inc. A Clinical Stage Pharmaceutical Company
Reinforcing the molecules of life Retrotope, Inc. A Clinical Stage Pharmaceutical Company Retrotope has created a proprietary drug platform to preserve and restore cellular health in degenerative diseases.
More informationMyeloperoxidase-generated reactive nitrogen species convert LDL into an atherogenic form in vitro
Myeloperoxidase-generated reactive nitrogen species convert LDL into an atherogenic form in vitro Eugene A. Podrez,, Henry F. Hoff, Stanley L. Hazen J Clin Invest. 1999;103(11):1547-1560. https://doi.org/10.1172/jci5549.
More informationComparison of Glucose & Lipid Profiles in Oxidative Stress Contain Diabetic Patients
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-issn: 2279-0853, p-issn: 2279-0861. Volume 4, Issue 4 (Jan.- Feb. 2013), PP 40-44 Comparison of Glucose & Lipid Profiles in Oxidative Stress Contain
More informationGeneral Biochemistry-1 BCH 202
General Biochemistry-1 BCH 202 1 I would like to acknowledge Dr. Farid Ataya for his valuable input & help in this course. 2 Outline Lipids Definition, function, fatty acids, classification: simple lipids:
More informationVDR GENE (Vitamin D Receptor)
12 GENES EXPLAINED. VDR GENE (Vitamin D Receptor) What is the purpose of the VDR gene? The Vitamin D receptor gene influences the ability to efficiently use vitamin D. Healthy bone density and strength
More informationFluoro Cholesterol Total Cholesterol Assay Kit
Fluoro Cholesterol Total Cholesterol Assay Kit Contact Information Address Telephone Toll Free Fax General Information Sales Technical Questions Website Cell Technology Inc 950 Rengstorff Ave Suite D Mountain
More informationInhibition of Lipid Peroxidation in Synaptosomes and Liposomes by Nitrates and Nitrites
Article Subscriber access provided by Columbia Univ Libraries Inhibition of Lipid Peroxidation in Synaptosomes and Liposomes by Nitrates and Nitrites Adrian C. Nicolescu, Sergei I. Zavorin, Nicholas J.
More informationAN ACTIVE SHELTER TO PROTECT C ELLS CELLS
AN ACTIVE SHELTER TO PROTECT CELLS v5 WHERE IS THE MARKET GOING TO? Niche segments, such as teenagers and men, are growing in the skin care market. They are also following the trends settled by women s
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2003 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2003) offered
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationAn analysis of accelerated atherosclerosis in the hyperglycemic hyperlipidemic Syrian F(1)B hamster
University of New Hampshire University of New Hampshire Scholars' Repository Master's Theses and Capstones Student Scholarship Spring 2007 An analysis of accelerated atherosclerosis in the hyperglycemic
More information