About OMICS International Conferences
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1 About OMICS Group OMICS Group is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information on Sciences and technology Open Access, OMICS Group publishes 500 online open access scholarly journals in all aspects of Science, Engineering, Management and Technology journals. OMICS Group has been instrumental in taking the knowledge on Science & technology to the doorsteps of ordinary men and women. Research Scholars, Students, Libraries, Educational Institutions, Research centers and the industry are main stakeholders that benefitted greatly from this knowledge dissemination. OMICS Group also organizes 500 International conferences annually across the globe, where knowledge transfer takes place through debates, round table discussions, poster presentations, workshops, symposia and exhibitions.
2 About OMICS International Conferences OMICS International is a pioneer and leading science event organizer, which publishes around 500 open access journals and conducts over 500 Medical, Clinical, Engineering, Life Sciences, Pharma scientific conferences all over the globe annually with the support of more than 1000 scientific associations and 30,000 editorial board members and 3.5 million followers to its credit. OMICS Group has organized 500 conferences, workshops and national symposiums across the major cities including San Francisco, Las Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai.
3 HDL Signaling in Macrophages and its role in atherosclerosis protection Bernardo Trigatti Department of Biochemistry and Biomedical Sciences Thrombosis and Atherosclerosis Research Institute McMaster University Hamilton, Canada 3 rd International Conference on Integrative Biology August 5, 2015, Valencia, Spain
4 Acknowledgements Monty Krieger Cambridge, USA Rick Austin Hamilton, Canada Suleiman Igdoura Hamilton, Canada Geoff Werstuck Hamilton, Canada Richard Lehner Edmonton, Canada Dennis Vance -- Edmonton, Canada Former members Aishah Al-Jarallah Scott Covey Omid Dadoo Kelvyn Fernades Mark Fuller Ying Pei Ali Rizvi Vivienne Tedesco David Wang Yi Zhang Current members Yazeed Alshuweishi Kristina Durham Leticia Gonzalez Melissa MacDonald Jane McBride Pei Yu Khosrow Adeli Toronto, Canada Gary Lewis Toronto, Canada Canadian Institutes of Health Research Funders Heart and Stroke Foundation of Canada
5 Atherosclerosis Necrotic Core Thrombus Ruptured Fibrous Cap Slide Source: Lipids Online Slide Library
6 Heart disease risk versus LDL and HDL-cholesterol levels
7 Low and High Density Lipoproteins LDL HDL Apolipoproteins Cholesterol Esters Triglycerides Phospholipids Cholesterol
8 HDL: Reverse cholesterol transport and signaling Degradation (Kidney) migration enos apoptosis HDL apoa1 ABCA1 SR-B1 ABCG1 Liver Hepatocytes blood Vascular Endothelium vascular wall Macrophage Foam Cell
9 Atherosclerosis Necrotic Core Thrombus Ruptured Fibrous Cap Slide Source: Lipids Online Slide Library
10 Macrophages resist ER-stress induced apoptosis 40% Annexin V TUNEL Pe ercent apoptosis Percent apoptosis 35% 30% 25% 20% 15% 10% 5% 0% 35% 30% 25% 20% 15% 10% 5% Thapsigargin Serum 0% - + Newborn Calf
11 Macrophages resist ER-stress induced apoptosis but become sensitive when lipoproteins are removed from serum Annexin V Pe ercent apoptosis 40% 35% 30% 25% 20% 15% 10% 5% 0% 35% TUNEL Percent apoptosis 30% 25% 20% 15% 10% 5% Thapsigargin Serum 0% Newborn Calf Newborn Calf Lipoprotein Deficient Pei Yu
12 HDL but not LDL suppresses ER-stress induced apoptosis in macrophages 25% Percent Ap poptosis 20% 15% 10% 5% 0% HDL (µg/ml) LDL (µg/ml) Tunicamycin Pei Yu
13 HDL signaling in macrophages SR-B1 HDL S1PR1 PDZK1 Gα i PI3K Akt 1 Bim Apoptosis Necrotic core Plaque instability
14 HDL treatment of macrophages reduces levels of the pro-apoptotic factor Bim HDL (min) Bim β-actin Yi Zhang, Aishah Al-Jarallah
15 HDL mediated suppression of ER stress- induced apoptosis requires SR-B1 % Apoptoti ic Cells Free Chol Load HDL Wild Type * * * NS % Apoptot tic Cells SR-B1 KO Yi Zhang, Aishah Al-Jarallah
16 HDL is unable to suppress ER stress-induced apoptosis in the absence of PDZK1 40% Wild Type PDZK1 KO Percent Apop ptosis 30% 20% 10% 0% Tunicamycin HDL Pei Yu
17 S1P is required for HDL mediated protection of macrophages from apoptosis S1P Lyase Reaction S1P S1P Lyase H Fatty aldehyde Pei Yu O + Leticia Gonzalez Phosphoethanolamine Tunicamycin Apoptosis (Clea aved Caspase 3) Control Treated HDL S1P-Lyase Treated HDL
18 Knockout of S1PR1 in macrophages reduces HDL mediated protection against ER-stress induced apoptosis Control S1PR1 KO Percent Apop ptosis Tunicamycin HDL Leticia Gonzalez
19 HDL signaling in macrophages SR-B1 HDL S1PR1 PDZK1 Gα i PI3K Akt 1 Bim Apoptosis Necrotic core Plaque instability
20 Measurement of atherosclerosis in mice Aortic Sinus Top Cut Away 20th U.S. edition of Gray's Anatomy of the Human Body, 1918
21 Increased Bim immunofluorescence in atherosclerotic plaques of mice lacking SR-B1 in bone marrow derived cells Bone Marrow SR-B1 B1 +/+ SR-B1 -/- nce s) Bim Fluorescen (arbitrary units SR-B1 * B1 +/+ SR-B1 -/- Yi Zhang, Aishah Al-Jarallah
22 Increased apoptosis in atherosclerotic plaques of mice lacking SR-B1 in bone marrow derived cells Bone Marrow SR-B1 B1 +/+ L * SR-B1 -/- L Apoptotic Nuclei/µm Apoptosis Nuclei SR-B1 B1 +/+ SR-B1 -/- Yi Zhang, Aishah Al-Jarallah
23 Increased necrotic core size in atherosclerotic plaques of mice lacking SR-B1 in bone marrow derived cells Bone Marrow SR-B1 B1 +/+ L L % Necrotic Cor re Necrotic Core Size * * SR-B1 -/- 0.0 SR-B1 B1 +/+ SR-B1 -/- Apoptosis Nuclei Yi Zhang, Aishah Al-Jarallah
24 Summary SR-B1 HDL S1PR1 Bim Apoptosis PDZK1 PI3K Gα i Thrombus Necrotic Core Ruptured Fibrous Cap Akt 1 Slide Source: Lipids Online Slide Library
25
26 Inactivation of SR-B1 in All Tissues of LDL Receptor -/- Mice Results in Diet-Induced Coronary Artery Atherothrombosis LDL r -/- SR-BI -/ - LDL r -/- Oil red O (Lipids) CD41 (red) Platelets 15 % Fat, 1.25 % Cholesterol, 0.5 % Cholate; 3.5 weeks Mark Fuller
27 SR-BI-/-LDL R-/- Mice Fed High Fat, High Cholesterol Diet Develop Extensive Cardiac Fibrosis LDL r -/- healthy SRSR-BI-/-LDL r -/- Infarcted fibrotic 15 % Fat, 1.25 % Cholesterol, 0.5 % Cholate; Cholate; 3.5 weeks; trichrome stain Mark Fuller
28 Reduced Survival of SR-BI -/ - LDLr -/ - mice Fed an Atherogenic Diet Survi ival 100% 75% 50% 25% LDL r -/- SR-BI -/ - LDL r -/- 0% Weeks on Diet 15 % Fat, 1.25 % Cholesterol, 0.5 % Cholate Mark Fuller
29 Let Us Meet Again We welcome you all to our future conferences of OMICS International Please Visit: biology-conferences.php
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS International Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group. OMICS Group International is an amalgamation of Open Access acetyl-coa
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 27 with the sole aim of making
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access Publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
About OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access Publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 27 with the sole aim of making
OMICS Group is an amalgamation of Open Access Publications and worldwide international science conferences and events. Established in the year 2007
* OMICS Group is an amalgamation of Open Access Publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information on Sciences
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation ofopen Access publicationsand worldwide international science conferences and events. Established in the year 2007 with the sole aim of making
OMICS International Conferences
About OMICS Group OMICS Group is an amalgamation of Open Access Publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information
OMICS Group International is an amalgamation of Open Access publications
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
About OMICS Group Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
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About OMICS International Conferences
About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of
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