Vector Newcastle vaccine usage in Latin America. Luiz Sesti, Technical Services Latin America Ceva, Brazil

Size: px

Start display at page:

Download "Vector Newcastle vaccine usage in Latin America. Luiz Sesti, Technical Services Latin America Ceva, Brazil"

Antonia Carpenter
5 years ago
Views:

1 Vector Newcastle vaccine usage in Latin America. Luiz Sesti, Technical Services Latin America Ceva, Brazil

2 Countries with endemic velogenic Newcastle Disease (ND) in Latin America Mexico Guatemala Belize El Salvador Honduras Dominican Republic Venezuela Colombia Ecuador Peru Bolivia Newcastle Velogénico en Latinoamérica OIE, 2013

OIE, 2013 PERU Medium to low field challenge from a very virulent field strain (ICPI 1.88).

3 Newcastle Velogénico en Latinoamérica Distinct ND epidemiological realities MEXICO High field challenge from very virulent field strains (ICPI ). OIE, 2013 PERU Medium to low field challenge from a very virulent field strain (ICPI 1.88). BRAZIL No field challenge from velogenic strains, medium to low challenge from lentogenic vaccine strains in the northeast region only.

4 Broiler ND vaccination MEXICO High field challenge by very virulent field strains (ICPI ). PERU Medium to low field challenge by a very virulent field strain (ICPI 1.88). 100% Very heavy protocols Protect against high mortality No concern for post vaccination reactions 100% Heavy to medium protocols Protect against high mortality Some concern for post vaccination reactions BRAZIL No field challenge from velogenic strains, medium to low challenge by lentogenic vaccine strains. Only 25% of all broilers Very light vaccination protocols Protect against eventual challenge from backyard chickens High concern for post vaccination reactions

5 Large field trials with the vector vaccine Vectormune ND (VTM ND) Representative trials from each country will be reviewed

TRIALS IN MEXICO MEXICO Field trials with commercial layers. Several field and controlled challenge trials carried out during the last 3 years in broilers.

6 TRIALS IN MEXICO MEXICO Field trials with commercial layers. Several field and controlled challenge trials carried out during the last 3 years in broilers. Broilers conventional vaccination programs (control groups) usually included: 3-4 live doses (day 1 = apathogenic or HB1 strains and field = La Sota mostly ED/spray) and 1-2 killed doses (day 1 and field - SQ) VTM ND at day one replaces the killed vaccines in both 1 and 2 doses protocols, conventional live program remains the same. Controlled lab challenge usually carried in broilers taken from the farms at days of age and monitored for 14 days after the challenge (Challenge Chimalhuacán strain, ICPI = 1.89, dose = 6 log 10 EID 50, eye drop route)

7 MEXICO Vaccination protocols Vectormune ND Control Field ND challenge 1 VTM ND + Live day 1 Live 15 days Killed + Live day 1 Live 15 days High 2 VTM ND + Live day 1 Live 12/23 days Killed + Live day 1 Killed 12 days Live 12/23 days Very high 3 VTM ND + Live day 1 Live 12/28 days Killed + Live day 1 Killed 12 days Live 12/28 days Very high

8 Commercial layers data to be presented by Dr. Rubén Ambario

9 8 broiler field trials % Livability after controlled lab challenge in broilers taken from the farms MEXICO average 100 % average % % VTM ND Control Non vaccinated SPF birds Vaccination Program

10 Reduction of ND challenge virus excretion in broilers vaccinated with VTM ND in comparison with broilers vaccinated with conventional ND vaccines in Mexico. ND challenge Chimalhuacán strain Genotype 5, ICPI = 1.89 Dose = 6 log 10 EID 50 eye drop Measured by quantitative RT-PCR in tracheal and cloacal swabs MEXICO TRIAL VACCINE % REDUCTION A VTM ND % Conventional B VTM ND % Conventional C VTM ND % Conventional D VTM ND - 100% Conventional E VTM ND % Conventional F VTM ND % Conventional

11 TRIALS IN MEXICO FINAL COMMENTS MEXICO VTM ND protected equally and sometimes better than the most heavy ND conventional vaccination program against a severe experimental ND challenge. VTM ND eliminated the need of killed vaccines providing an better opportunity for growth (less metabolic costs). And most prominently, VTM ND induced a significantly reduction in challenge virus excretion and thus, providing a reduced environmental contamination. This may, along with improvements in other biosecurity measures, decrease field challenge in the median to long run and again providing a better environment for growth performance.

12 TRIAL IN PERU PERU Large broiler field trial in a low to medium velogenic ND challenge area. Main objectives were: Eliminate field vaccination (post vaccination reactions) Maintain ND protection Possibly improve broiler performance Data collected broiler performance Income generated serology

13 PERU Vaccination protocols Vectormune ND Control VTM ND + Live day 1 (spray) Live day 1 (spray) Live day 10 (DW) Live day 20 (DW) VTM ND = 995,250 broilers Control = 950,275 broilers 100% contemporary groups

14 PERU Broiler performance Age (days) Mortality (%) a 41.2 a 7,5 4,5 6 1, a 3.7 b Production efficiency Index Control VTM HVT-NDV Control VTM HVT-NDV b Feed conversion (g/g) Final weight (kg) a 1,7 1, a 1.68 b 2,5 2 1, a 2.49 b Control VTM HVT-NDV Control VTM HVT-NDV Control VTM HVT-NDV

15 Cost:Benefit analysis between groups PERU Day old chick cost Feed cost Vaccination cost Medication cost Livability Slaughter age Feed Conversion (FC) Sales price (slaughter broiler) FC adjustment Weight adjustment

16 US$ 217 dollars EXTRA income per 1000 broilers vaccinated with VTM ND total of US$ 216,000 in this trial

Serology PERU 3000 2802 2500 2000 1500 1000

(idexx) 7 14 21 28 35 42 Log 2 5 4 3 2 4.2 2.

17 Serology PERU cutoff ND Elisa (idexx) Log cutoff ND HI

18 TRIAL IN PERU FINAL COMMENTS PERU VTM ND protected equally as the ND conventional vaccination program used (no ND break in both groups). VTM ND vaccinated broilers presented a significantly better clinical and growth performance. VTM ND vaccinated broilers generated a quite important extra income when compared to the final income generated by the control group.

19 BRAZIL TRIALS IN BRAZIL Several large field trials were carried out in nonchallenge areas for velogenic ND in a low to medium velogenic ND challenge area. Main objectives were: Eliminate field vaccination (post vaccination reactions) Possibly improvement broiler performance Data collected broiler performance Income generated serology

BRAZIL Broiler ND vaccination in Brazil Northeast Southeast South Current broiler production = 560 mi / month Only 25% ND vaccinated (20% northeast / 5% southeast) South area = 28 years without

20 BRAZIL Broiler ND vaccination in Brazil Northeast Southeast South Current broiler production = 560 mi / month Only 25% ND vaccinated (20% northeast / 5% southeast) South area = 28 years without broiler ND vaccination (responsible for 53% of all broiler production) Recent comprehensive surveys (serology, virology, molecular detection) found no velogenic NDV in industrial and backyard poultry (Orsi et al. 2010; Thomazelli et al. 2012) Clinical signs and their consequences in the poultry industry are 100% related to lentogenic vaccine virus circulation ( rolling reactions)

21 Field trials BRAZIL

22 BRAZIL Companies 1, 3, 4 Newcastle Vaccination program Company 1 Hatchery Field Control apath. Phy.LMV.42 (spray) La Sota (DW) 18 days rhvt - NDV rhvt NDV (SQ) Company 3 Hatchery Field Control apath. Phy.LMV.42 (spray) rhvt NDV rhvt NDV (SQ) Company 4 Hatchery Field Control C2 (spray) rhvt - NDV rhvt NDV (SQ)

23 Broiler Performance BRAZIL

24 BRAZIL Cost:Benefit Analysis between groups Day old chick cost Feed cost Vaccination cost Medication cost Livability Slaughter age Feed Conversion (FC) Sales price (slaughter broiler) FC adjustment Weight adjustment

VTM ND vaccinated broilers BRAZIL US$ 90 80 70 60 50 40 30 20 10 0 85 Extra income per

25 VTM ND vaccinated broilers BRAZIL US$ Extra income per 1000 broilers (US$) Company 1 Company 3 Company 4 females [VALOR] Company 4 males

BRAZIL Trachea histopathology lesion scores per age Treaments rhvt- NDV Control rhvt- NDV Control rhvt- NDV Control 14 days of age 21 days of age 28 days of

26 BRAZIL Trachea histopathology lesion scores per age Treaments rhvt- NDV Control rhvt- NDV Control rhvt- NDV Control 14 days of age 21 days of age 28 days of age Company a 0.43 a 0.53 a 0.60 b 0.69 a 0.79 b Company a 0.52 b 0.66 a 0.86 b 0.69 a 0.99 b Company a 0.33 b 0.35 a 0.45 b 0.56 a 0.60 a

27 ,4 6,4 6,3 5,1 6,8 5,2 1,5 1,4 1,4 1,6 1,7 1,5 rhvt - NDV Control HI Newcastle > 3 log 2 = pos 1 1,1 1 1,4 1,2 1 1,7 2,4 1,1 1,7 1,2 1 2 BRAZIL Serology HI (GMT Log2) for Newcastle (Companies 1, 3 e 4) 1. apath. Phy.LMV.42 + La Sota 3. apath. Phy.LMV C2 (lentogenic) 3,1 1,3 2,3 1,9 1 0 V1 V1 V1 C1 C1 C1 V14 V14 V14 C14 C14 C14 V21 V21 V21 C21 C21 C21 V35 V35 V35 C35 C35 C35 V42 V42 V42 C42 C42 C42 Day 1 Day 14 Day 21 Day 35 Day 42

28 Serology Elisa (Idexx) for Newcastle (Companies 1, 3 e 4) BRAZIL rhvt - NDV Control 1. apath. Phy.LMV.42 + La Sota 3. apath. Phy.LMV C2 (lentogenic) Elisa Newcastle > 400 = pos V1 V1 C1 V14 V14 C14 V21 V21 C21 V35 V35 C35 V42 V42 C42 V49 V49 C Day 1 Day 14 Day 21 Day 35 Day 42 Day 49

TRIALS IN BRAZIL FINAL COMMENTS BRAZIL Production performance of VTM ND vaccinated broilers was either statistically better or presented a strong trend of improvement in all trials.

29 TRIALS IN BRAZIL FINAL COMMENTS BRAZIL Production performance of VTM ND vaccinated broilers was either statistically better or presented a strong trend of improvement in all trials. Better clinical performance for VTM ND vaccinated broilers was consistently reported by caretakers in all companies. Financial results were always better to VTM ND vaccinated broiler flocks. Elisa seroconversion was evident, of low magnitude and very uniform from 5 weeks of age. The safety of VTM ND has been clearly demonstrated in comparison with conventional live ND vaccines by tracheas' histopathology lesion score analysis (no "rolling" post vaccination reactions). VTM ND has been proved to be the safest immunological tool for ND prevention in countries with no velogenic NDV.

30 FINAL COMMENTS After being tested in three totally different Newcastle Disease epidemiological situations the vector HVT NDV vaccine Vectormune ND has been clearly shown to be: significantly effective for the clinical protection against Newcastle Disease and certainly the safest immunological tool to for prevention of Newcastle Disease With such a tool, many countries around the world will have a quite better opportunity to work on ND eradication programs.

CEVA MEXICO Justino Hernandez Mario Lechuga

Rosemberg Roberto Valdivia Juan Carlos Romero CEVA

31 CEVA MEXICO Justino Hernandez Mario Lechuga Francisco Martínez Maurício Gonzalez David Dueñas Jesus Reyes CEVA PERU Pedro Patrício Jorge Cortegana Yesenia Vega Rocio Orosco Ricardo Rosemberg Roberto Valdivia Juan Carlos Romero CEVA BRAZIL Ricardo Pereira Carlos Kneipp Rodrigo Paranhos Amaro Borges Alberto Inoue

32 Thanks so much! Luiz Sesti

RECOMBINANT VACCINES Live or Killed

Antigens and Vaccines Molecular biology drives Innovation Antigen Gene of Interest Recombination RECOMBINANT VACCINES Live or Killed Antigens and Vaccines Molecular biology drives Innovation Antigen Gene