mrna Vaccination with Charge- Altering Releasable Transporters Cures Established Experimental Tumors
|
|
|
- Judith Caldwell
- 5 years ago
- Views:
Transcription
1 mrna Vaccination with Charge- Altering Releasable Transporters Cures Established Experimental Tumors Dr. Timothy R. Blake Cancer TNT Quarterly Meeting 5/4/18
2 Principles of cancer vaccination Introduction of antigen/protein to APCs is the critical first step, afterward the immune system can take over mrna-based vaccination was proposed to be an ideal approach in the early 1970s Current methods include ex vivo activation by electroporation or viral delivery Efficient mrna transfection avoids expensive, invasive treatments
3 Nanoparticle-based mrna delivery Effective and non-toxic delivery of mrna is the major challenge of gene therapy Recent efforts using mrna delivery have shown remarkable utility as a new class of prodrug Jun Cancer vaccine (BioNTech) Nov Dec Cancer vaccine CRISPR (Moderna) (Moderna) Mar Hemophilia (Arcturas) Mar ZIKA vaccine (BioNTech) *All use proprietary Lipid Nanoparticles (LNPs) Which by Lipid nanoparticles (LNPs) Many components (some toxic) Component selection is non trivial Require microfluidic formulation Biodegradable polyplexes Amphipathic cationic oligomers Electrostatic complexation with mrna Successful with sirna
4 Synthesis or Charge-Altering Releasable Transporters (CARTs) R OH Lipid: + O O O O O n Lipid Urea Alkoxide Catalyst O OK N N H O NBoc m O R O O O n O O Lipid O O R O O O n O O Lipid O Boc N HCl Cl H 2 N CART O H m O H m Robust straightforward chemistry Fluorophores and targeting ligands can be incorporated Tunable for: sirna delivery pdna delivery Organ targeting Mix and shoot
5 Charge-Altering Releasable Transporters (CARTs) protect, deliver, and release mrna intracellularly Fluorophores - Dansyl: attached to transporter mrna complexation Intracellular delivery and cytosolic release Gene Expression - GFP: indicates expression of mrna - Cy5: attached to mrna Endocytosis charge-altering mechanism osmotic rupture translation mrna-binding by CART CART-mRNA complex endosome w/ complex endosome w/ mrna and HEGD mrna release therapeutic protein expression Endocytosis no significant degradation endosomal acidification acidic and/or enzymatic degradation mrna-binding by non-releasing transporter non-releasing transportermrna complex endosome w/ complex endosome w/ complex late endosome/ lysosome inactivated mrna extracellular intracellular Major limitation for mrna delivery is endosomal escape
6 CARTs transfect cells in vivo via multiple routes of administration IV administration shows robust uptake by the spleen and lymph nodes
7 Fluorophore labeled CARTs allow for phenotypic identification of transfected cells Blood samples taken after 1hr Spleens isolated/digested after 1 hr Skin at the site of SC injection digested at 1 hr Stained for FACS
8 CART-based mrna vaccination CARTs alone demonstrate low immunogenicity However, simply adding in a polyanionic adjuvant gives CART/mRNA vaccines enhances the desired immuno-stimulatory effect *Adjuvant (Adj.) short nucleotide PAMP (pathogen associated molecular pattern) Cells alone CART/mRNA (mcherry) CART/mRNA (mcherry) +FITC-adj. FITC-adj. alone
9 Ovalbumin-expressing A20 lymphoma model OVA Subcutaneous inoculation of 10 7 ovalbumin-expressing A20 cells Mice sacrificed upon tumor reaching a volume of 1000 mm 3 Readout: Tumor size and survival Wait 7 days Prophylactic treatment: Single dose of 7.5ug CART/mRNA/adj. mrna/cart Tumor volum measured at ea timepoint Therapeutic treatment: 3x3 ug dose of CART/mRNA/adj.
10 CART-mRNA vaccine gives long lasting prophylactic protection against OVA-A20 cells T-cells isolated from spleen Used for ex-vivo A20 cell killing assay Adj.
11 Antigen specific ex vivo killing of cancer cells Adj. Adj.
12 IV vaccination with CART/mRNA cures established tumors Adj. Adj.
13 CART-mRNA vaccine cures established tumors Adj. Adj.
14 Conclusions CARTs effectively transfect cells in vivo and express mrna cargo CARTs show preferential uptake in the spleen, where a high percentage of APCs are transfected with antigenic prodrugs (mrna) T-Cells of vaccinated mice kill antigen expressing A20 cells ex vivo The immunity granted by mrna/cart/adjuvant vaccines can cure established tumors in mice
15 Acknowledgements Prof. Robert M. Waymouth Prof. Paul A. Wender Prof. Ronald Levy Colin J. McKinlay Ole Audun Werner Haabeth Waymouth Lab Levy Lab Wender Lab Stanford University
AsiaTIDES 2012: Formulation and Delivery of Peptides and Oligonucleotides Strategies for Delivery of RNAi Therapeutics. March 1, 2012 Akin Akinc, PhD
AsiaTIDES 2012: Formulation and Delivery of Peptides and Oligonucleotides Strategies for Delivery of RNAi Therapeutics March 1, 2012 Akin Akinc, PhD Lead Selection Alnylam RNAi Product Platform Turning
Cationic Liposome-DNA Complexes as Immunomodulators and Vaccine Adjuvants
Cationic Liposome-DNA Complexes as Immunomodulators and Vaccine Adjuvants September 29, 28 Platform Technology Proprietary Cationic Lipid-DNA Complexes Cationic/Neutral Lipid + DNA = JVRS-1 JVRS-1 + Antigen
Antigen presenting cells
Antigen recognition by T and B cells - T and B cells exhibit fundamental differences in antigen recognition - B cells recognize antigen free in solution (native antigen). - T cells recognize antigen after
Chapter 22: The Lymphatic System and Immunity
Bio40C schedule Lecture Immune system Lab Quiz 2 this week; bring a scantron! Study guide on my website (see lab assignments) Extra credit Critical thinking questions at end of chapters 5 pts/chapter Due
BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003
Lecture 16: Intracellular drug delivery Last time: Today: nano- and micro-particle drug carriers Delivery to tissues from systemic circulation Intracellular drug delivery Reading: A.S. Hoffman et al.,
General information. Cell mediated immunity. 455 LSA, Tuesday 11 to noon. Anytime after class.
General information Cell mediated immunity 455 LSA, Tuesday 11 to noon Anytime after class T-cell precursors Thymus Naive T-cells (CD8 or CD4) email: lcoscoy@berkeley.edu edu Use MCB150 as subject line
M.Sc. III Semester Biotechnology End Semester Examination, 2013 Model Answer LBTM: 302 Advanced Immunology
Code : AS-2246 M.Sc. III Semester Biotechnology End Semester Examination, 2013 Model Answer LBTM: 302 Advanced Immunology A. Select one correct option for each of the following questions:- 2X10=10 1. (b)
VIROMER ONE. standardized transfection reagent
VIROMER ONE standardized transfection reagent 1 Doing transfection is like making coffee. Same procedure, somewhat different result each time you do it. Then came Nespresso. An easy, comfortable and clean
CELL BIOLOGY - CLUTCH CH THE IMMUNE SYSTEM.
!! www.clutchprep.com CONCEPT: OVERVIEW OF HOST DEFENSES The human body contains three lines of against infectious agents (pathogens) 1. Mechanical and chemical boundaries (part of the innate immune system)
NANO 243/CENG 207 Course Use Only
L4: Nanomedicine in Immunotherapy April 12, 2018 Branches of the Immune System Body has two main branches of immunity, innate and adaptive. Innate immunity is the first line of defense. Phagocytes such
THE ROLE OF ALTERED CALCIUM AND mtor SIGNALING IN THE PATHOGENESIS OF CYSTINOSIS
Research Foundation, 18 month progress report THE ROLE OF ALTERED CALCIUM AND mtor SIGNALING IN THE PATHOGENESIS OF CYSTINOSIS Ekaterina Ivanova, doctoral student Elena Levtchenko, MD, PhD, PI Antonella
Chapter 6. Antigen Presentation to T lymphocytes
Chapter 6 Antigen Presentation to T lymphocytes Generation of T-cell Receptor Ligands T cells only recognize Ags displayed on cell surfaces These Ags may be derived from pathogens that replicate within
Antigen Presentation and T Lymphocyte Activation. Abul K. Abbas UCSF. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Abul K. Abbas UCSF FOCiS 2 Lecture outline Dendritic cells and antigen presentation The role of the MHC T cell activation Costimulation, the B7:CD28 family
Figure S1. Western blot analysis of clathrin RNA interference in human DCs Human immature DCs were transfected with 100 nm Clathrin SMARTpool or
Figure S1. Western blot analysis of clathrin RNA interference in human DCs Human immature DCs were transfected with 100 nm Clathrin SMARTpool or control nontargeting sirnas. At 90 hr after transfection,
COURSE: Medical Microbiology, MBIM 650/720 - Fall TOPIC: Antigen Processing, MHC Restriction, & Role of Thymus Lecture 12
COURSE: Medical Microbiology, MBIM 650/720 - Fall 2008 TOPIC: Antigen Processing, MHC Restriction, & Role of Thymus Lecture 12 FACULTY: Dr. Mayer Office: Bldg. #1, Rm B32 Phone: 733-3281 Email: MAYER@MED.SC.EDU
Institute t of Experimental Immunology University of Bonn, Germany
How T cells recognize antigen Christian Kurts Institute t of Experimental Immunology University of Bonn, Germany When do T cells recognize antigen? Antigen uptake Activation phase 1st recognition Effector
TCR, MHC and coreceptors
Cooperation In Immune Responses Antigen processing how peptides get into MHC Antigen processing involves the intracellular proteolytic generation of MHC binding proteins Protein antigens may be processed
Antigen Presentation to T lymphocytes
Antigen Presentation to T lymphocytes Immunology 441 Lectures 6 & 7 Chapter 6 October 10 & 12, 2016 Jessica Hamerman jhamerman@benaroyaresearch.org Office hours by arrangement Antibodies and T cell receptors
T-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
T-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
Medical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University
Medical Virology Immunology Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University Human blood cells Phases of immune responses Microbe Naïve
Anonymous MIT students MK and NM Natalie Kuldell May 2009
Anonymous MIT students MK and NM Natalie Kuldell 20.020 13 May 2009 Technical Document: oncocures Problem: Dynamic Metastasis Imaging Our project has been designed with the hope of creating a way to detect
LAMPvax DNA Vaccines as Immunotherapy for Cancer - Three Case Studies
LAMPvax DNA Vaccines as Immunotherapy for Cancer - Three Case Studies Cancer immunotherapy has emerged as a clinically validated tool for fighting certain kinds of cancers. These therapeutic cancer vaccines
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Supplementary Figure 1: Chemokine receptor expression profiles of CCR6 + and CCR6 - CD4 + IL-17A +/ex and Treg cells. Quantitative PCR analysis of chemokine receptor transcript abundance
Spherical Nucleic Acids For Advanced Wound Healing Applications Chad A. Mirkin
Spherical Nucleic Acids For Advanced Wound Healing Applications Chad A. Mirkin Departments of Chemistry, Infectious Disease, Materials Science & Engineering, Chemical & Biological Engineering, and Biomedical
Boosting intracellular delivery of lipid nanoparticle-encapsulated messenger RNA
Supplementary Information (additional figures and tables) Boosting intracellular delivery of lipid nanoparticle-encapsulated messenger RNA 1 Siddharth Patel, 1 N Ashwanikumar, 1 Emily Robinson, 1 Allison
ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY
ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY The recognition of specific antigen by naïve T cell induces its own activation and effector phases. T helper cells recognize peptide antigens through
Dendritic cells in cancer immunotherapy Aimin Jiang
Dendritic cells in cancer immunotherapy Aimin Jiang Feb. 11, 2014 Dendritic cells at the interface of innate and adaptive immune responses Dendritic cells: initiators of adaptive immune responses Dendritic
ECM1 controls T H 2 cell egress from lymph nodes through re-expression of S1P 1
ZH, Li et al, page 1 ECM1 controls T H 2 cell egress from lymph nodes through re-expression of S1P 1 Zhenhu Li 1,4,Yuan Zhang 1,4, Zhiduo Liu 1, Xiaodong Wu 1, Yuhan Zheng 1, Zhiyun Tao 1, Kairui Mao 1,
Cover Page. The handle holds various files of this Leiden University dissertation
Cover Page The handle http://hdl.handle.net/1887/35908 holds various files of this Leiden University dissertation Author: Soema, Peter Title: Formulation of influenza T cell peptides : in search of a universal
Overview of the Immune System
Overview of the Immune System Immune System Innate (Nonspecific) Adaptive (Specific) Cellular Components Humoral Components Cell-Mediated Humoral (Ab) Antigens Definitions Immunogen Antigen (Ag) Hapten
Instructions. Fuse-It-mRNA easy. Shipping and Storage. Overview. Kit Contents. Specifications. Note: Important Guidelines
Membrane fusion is a highly efficient method for transfecting various molecules and particles into mammalian cells, even into sensitive and primary cells. The Fuse-It reagents are cargo-specific liposomal
Supplemental Figure 1. Cell-bound Cetuximab reduces EGFR staining intensity. Blood
Antibody-mediated depletion of CD19-CAR T cells Supplemental 1 Supplemental Materials Supplemental Figure 1. Supplemental Figure 1. Cell-bound Cetuximab reduces EGFR staining intensity. Blood cells were
Supplementary Figure 1. Deletion of Smad3 prevents B16F10 melanoma invasion and metastasis in a mouse s.c. tumor model.
A B16F1 s.c. Lung LN Distant lymph nodes Colon B B16F1 s.c. Supplementary Figure 1. Deletion of Smad3 prevents B16F1 melanoma invasion and metastasis in a mouse s.c. tumor model. Highly invasive growth
LabBOOK VIROMER RED VIROMER YELLOW. plasmid DNA /mrna transfection
LabBOOK VIROMER RED VIROMER YELLOW plasmid DNA /mrna transfection General information Technology Viromer RED and Viromer YELLOW are polymer-based transfection reagents featuring a viral mechanism of membrane
The Major Histocompatibility Complex (MHC)
The Major Histocompatibility Complex (MHC) An introduction to adaptive immune system before we discuss MHC B cells The main cells of adaptive immune system are: -B cells -T cells B cells: Recognize antigens
Dr. Ahmed K. Ali Attachment and entry of viruses into cells
Lec. 6 Dr. Ahmed K. Ali Attachment and entry of viruses into cells The aim of a virus is to replicate itself, and in order to achieve this aim it needs to enter a host cell, make copies of itself and
RXi Pharmaceuticals. Immuno-Oncology World Frontiers Conference. January 23, 2018 NASDAQ: RXII. Property of RXi Pharmaceuticals
RXi Pharmaceuticals Immuno-Oncology World Frontiers Conference January 23, 2018 NASDAQ: RXII Forward Looking Statements This presentation contains forward-looking statements within the meaning of the Private
Determinants of Immunogenicity and Tolerance. Abul K. Abbas, MD Department of Pathology University of California San Francisco
Determinants of Immunogenicity and Tolerance Abul K. Abbas, MD Department of Pathology University of California San Francisco EIP Symposium Feb 2016 Why do some people respond to therapeutic proteins?
genome edited transient transfection, CMV promoter
Supplementary Figure 1. In the absence of new protein translation, overexpressed caveolin-1-gfp is degraded faster than caveolin-1-gfp expressed from the endogenous caveolin 1 locus % loss of total caveolin-1-gfp
Bioassays for Quality Control of Cell & Gene Therapy Products
Bioassays for Quality Control of Cell & Gene Therapy Products Erik Rutjens, Cell & Gene Therapy, Novartis Pharma AG CASSS Bioassays, Silver Spring, March2015 CTL019 Introduction CARTs = Chimeric Antigen
Development of a VP6 subunit rotavirus vaccine A dual role of VP6 as a vaccine antigen and an adjuvant
30 August 2018, Minsk 13TH INTERNATIONAL ROTAVIRUS SYMPOSIUM Development of a VP6 subunit rotavirus vaccine A dual role of VP6 as a vaccine antigen and an adjuvant Dr. Vesna Blazevic Head of Laboratory
Virus Entry. Steps in virus entry. Penetration through cellular membranes. Intracellular transport John Wiley & Sons, Inc. All rights reserved.
Virus Entry Steps in virus entry Penetration through cellular membranes Intracellular transport Steps in virus entry How do virions get into cells? Viruses of bacteria, archaea, algae and plants use different
General Overview of Immunology. Kimberly S. Schluns, Ph.D. Associate Professor Department of Immunology UT MD Anderson Cancer Center
General Overview of Immunology Kimberly S. Schluns, Ph.D. Associate Professor Department of Immunology UT MD Anderson Cancer Center Objectives Describe differences between innate and adaptive immune responses
5.6 Diffusion, Membranes, and Metabolism
5.6 Diffusion, Membranes, and Metabolism Concentration of a substance Number of atoms or molecules in a given volume Concentration gradient of a substance A difference in concentration between two regions
Pre-made Lentiviral Particles for intracelular labeling: (LocLight TM Living cell imaging lentivirus for sub-cellular localization)
Pre-made Lentiviral Particles for intracelular labeling: (LocLight TM Living cell imaging lentivirus for sub-cellular localization) LocLight TM cell organelle labeling lentivirus is provided as 200ul/per
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Supplementary Figure 1. (A) Left, western blot analysis of ISGylated proteins in Jurkat T cells treated with 1000U ml -1 IFN for 16h (IFN) or left untreated (CONT); right, western
Using Modified Bacterial Toxins To Deliver Vaccine Antigens
Using Modified Bacterial Toxins To Deliver Vaccine Antigens Researchers are using toxins to deliver epitopes in candidate vaccines that specifically stimulate protective cytotoxic T lymphocyte responses
Supplementary Figure S1. Flow cytometric analysis of the expression of Thy1 in NH cells. Flow cytometric analysis of the expression of T1/ST2 and
Supplementary Figure S1. Flow cytometric analysis of the expression of Thy1 in NH cells. Flow cytometric analysis of the expression of T1/ST2 and Thy1 in NH cells derived from the lungs of naïve mice.
Protein Trafficking in the Secretory and Endocytic Pathways
Protein Trafficking in the Secretory and Endocytic Pathways The compartmentalization of eukaryotic cells has considerable functional advantages for the cell, but requires elaborate mechanisms to ensure
Light and X-ray triggered liposomal gene/drug delivery system for cancer therapy
Light and X-ray triggered liposomal gene/drug delivery system for cancer therapy Wei Deng, 1 Wenjie Chen, 2 Sandhya Clement, 1 Anna Guller, 1 Alexander Engel, 3 Ewa Goldys 1 1 The Graduate School of Biomedical
Live cell imaging of trafficking of the chaperone complex vaccine to the ER. BMDCs were incubated with ER-Tracker Red (1 M) in staining solution for
Live cell imaging of trafficking of the chaperone complex vaccine to the ER. BMDCs were incubated with ER-Tracker Red (1 M) in staining solution for 15 min at 37 C and replaced with fresh complete medium.
Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder I Nakaya, Kousik
SUPPLEMENTARY FIGURES 1-19 T H 2 response to cysteine-proteases requires dendritic cell-basophil cooperation via ROS mediated signaling Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder
Nanodevices for Treatment of Hyperlipidemia
Nanodevices for Treatment of Hyperlipidemia Vladimir Reukov, Daniel Carey and Alexey Vertegel Bioengineering Department, Clemson University, SC, USA Atherosclerosis is the leading cause of death in the
2. The normal of the gut, and vagina keep the growth of pathogens in check. 3. in the respiratory tract sweep out bacteria and particles.
Chapter 39 Immunity I. Three Lines of Defense A. Surface Barriers to Invasion 1. is an important barrier. 2. The normal of the gut, and vagina keep the growth of pathogens in check. 3. in the respiratory
Personalized Cancer Neoantigen Vaccines
Personalized Cancer Neoantigen Vaccines Turning the Immune System Against Your own Unique Tumour-Specific Antigens 3 rd Annual Advances in Immuno-Oncology Congress London, May 24, 2018 Agnete Fredriksen,
Introduction. Cancer Biology. Tumor-suppressor genes. Proto-oncogenes. DNA stability genes. Mechanisms of carcinogenesis.
Cancer Biology Chapter 18 Eric J. Hall., Amato Giaccia, Radiobiology for the Radiologist Introduction Tissue homeostasis depends on the regulated cell division and self-elimination (programmed cell death)
Trends in vaccinology
Trends in vaccinology Mathieu Peeters, MD Joint Conference of European Human Pharmacological Societies and Joint Conference of European Human Pharmacological Societies and 20th Anniversary of AGAH March
By:Reham Alahmadi NOV The production of antibodies and vaccination technology
By:Reham Alahmadi NOV 2018 The production of antibodies and vaccination technology Antibody Production The blood contains two types of white blood cell or leukocyte Phagocytes ingest bacteria by endocytosis
Innate Immunity: (I) Molecules & (II) Cells. Part II: Cells (aka the Sentinels)
Innate Immunity: (I) Molecules & (II) Cells Stephanie Eisenbarth, M.D., Ph.D. FOCIS Advanced Course 2/19/18 Department of Laboratory Medicine Yale School of Medicine Department of Immunobiology Yale School
(A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and a
Supplementary figure legends Supplementary Figure 1. Expression of Shh signaling components in a panel of gastric cancer. (A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and
(b) fluid returns to venous end of capillary due to hydrostatic pressure and osmotic pressure
(Slide 1) Lecture Notes: Lymphatic System and Immunity (Body Defenses) I. (Slide 2) Introduction A) General 1) Lymphatic System performs 2 basic functions: 2) Organs of the Lymphatic System (a) lymphatic
Intranasal DNA Vaccine for Protection against Respiratory Infectious Diseases: The Delivery Perspectives
Pharmaceutics 2014, 6, 378-415; doi:10.3390/pharmaceutics6030378 OPEN ACCESS pharmaceutics ISSN 1999-4923 www.mdpi.com/journal/pharmaceutics Review Intranasal DNA Vaccine for Protection against Respiratory
Supplementary Information:
Supplementary Information: Follicular regulatory T cells with Bcl6 expression suppress germinal center reactions by Yeonseok Chung, Shinya Tanaka, Fuliang Chu, Roza Nurieva, Gustavo J. Martinez, Seema
Intracellular Vesicular Traffic Chapter 13, Alberts et al.
Intracellular Vesicular Traffic Chapter 13, Alberts et al. The endocytic and biosynthetic-secretory pathways The intracellular compartments of the eucaryotic ell involved in the biosynthetic-secretory
RAISON D ETRE OF THE IMMUNE SYSTEM:
RAISON D ETRE OF THE IMMUNE SYSTEM: To Distinguish Self from Non-Self Thereby Protecting Us From Our Hostile Environment. Innate Immunity Acquired Immunity Innate immunity: (Antigen nonspecific) defense
The development and function of immune cells
The development and function of immune cells Our research focusses on the development and function of immune cells. For cells to develop and carry out their jobs properly, instructions must be read and
Supplementary Figure 1. NAFL enhanced immunity of other vaccines (a) An over-the-counter, hand-held non-ablative fractional laser (NAFL).
Supplementary Figure 1. NAFL enhanced immunity of other vaccines (a) An over-the-counter, hand-held non-ablative fractional laser (NAFL). (b) Depiction of a MTZ array generated by NAFL. (c-e) IgG production
mrna vaccines a new era in vaccinology
mrna vaccines a new era in vaccinology Norbert Pardi 1, Michael J. Hogan 1, Frederick W. Porter 2 and Drew Weissman 1 Abstract mrna vaccines represent a promising alternative to conventional vaccine approaches
Emerging Viruses. Part IIb Follow Up from Part I Vaccines and Inhibitors
Emerging Viruses Part IIb Follow Up from Part I Vaccines and Inhibitors Cellular Responses to Viral Invasion: Restriction Factors Cells fight viral infection using a series of restriction factors Restriction
In-San Kim, M.D., Ph.D. KIST InterMembrane Signaling Lab & KU-KIST, Korea University The 15 th Korea-US Forum on Nanotechnology,
In-San Kim, M.D., Ph.D. KIST InterMembrane Signaling Lab & KU-KIST, Korea University The 15 th Korea-US Forum on Nanotechnology, 2018. 7. 12 Annals of Oncol. 2018 Tang et al., 2004 new IO agents : 940
Opening Activity. Make a list of all the diseases and infections you have had.
Opening Activity Make a list of all the diseases and infections you have had. If you have had chicken pox, indicate whether you have had it more than once. Content Objectives I will be able to identify
Dendritic cell subsets and CD4 T cell immunity in Melanoma. Ben Wylie 1 st year PhD Candidate
Dendritic cell subsets and CD4 T cell immunity in Melanoma Ben Wylie 1 st year PhD Candidate Melanoma Melanoma is the 4 th most common cancer in Australia. Current treatment options are ineffective resulting
Immunology. T-Lymphocytes. 16. Oktober 2014, Ruhr-Universität Bochum Karin Peters,
Immunology T-Lymphocytes 16. Oktober 2014, Ruhr-Universität Bochum Karin Peters, karin.peters@rub.de The role of T-effector cells in the immune response against microbes cellular immunity humoral immunity
Molecular and Cellular Basis of Immune Protection of Mucosal Surfaces
Molecular and Cellular Basis of Immune Protection of Mucosal Surfaces Department of Biologic & Materials Sciences School of Dentistry University of Michigan Ann Arbor, Michigan 48109-1078 1 Image quality
A Multicistronic DNA Vaccine Induces Significant Protection against Tuberculosis in Mice and Offers Flexibility in the Expressed Antigen Repertoire.
Company LOGO A Multicistronic DNA Vaccine Induces Significant Protection against Tuberculosis in Mice and Offers Flexibility in the Expressed Antigen Repertoire. Fayaz Ahmad Mir, Stefan H. E. Kaufmann,
Innate Immunity. Chapter 3. Connection Between Innate and Adaptive Immunity. Know Differences and Provide Examples. Antimicrobial peptide psoriasin
Chapter Know Differences and Provide Examples Innate Immunity kin and Epithelial Barriers Antimicrobial peptide psoriasin -Activity against Gram (-) E. coli Connection Between Innate and Adaptive Immunity
General Biology. A summary of innate and acquired immunity. 11. The Immune System. Repetition. The Lymphatic System. Course No: BNG2003 Credits: 3.
A summary of innate and acquired immunity General iology INNATE IMMUNITY Rapid responses to a broad range of microbes Course No: NG00 Credits:.00 External defenses Invading microbes (pathogens). The Immune
PHARMACEUTICALLY ENGINEERED NANOPARTICLES FOR ENHANCING IMMUNE RESPONSES TO HIV-1 TAT AND GAG p24 PROTEINS
University of Kentucky UKnowledge University of Kentucky Doctoral Dissertations Graduate School 2006 PHARMACEUTICALLY ENGINEERED NANOPARTICLES FOR ENHANCING IMMUNE RESPONSES TO HIV-1 TAT AND GAG p24 PROTEINS
Point of Care Tests for respiratory viruses: impact on clinical outcomes of patients
Point of Care Tests for respiratory viruses: impact on clinical outcomes of patients Dr Sacha Stelzer-Braid Postdoctoral Scientist Virology Research Laboratory (VRL), UNSW and POWH @DrSachaSB Outline 1.
Human metapneumovirus (hmpv) and parainfluenza virus 3 (PIV3) vaccine (mrna-1653)
Human metapneumovirus (hmpv) and parainfluenza virus 3 (PIV3) vaccine (mrna-1653) Conference Call February 12, 2019 Flu H10N8 H7N9 Prophylactic vaccines RSV VZV CMV hmpv+piv3 Chikungunya VLP Zika VLP Cancer
Production of Exosomes in a Hollow Fiber Bioreactor
Production of Exosomes in a Hollow Fiber Bioreactor John J S Cadwell, President and CEO, FiberCell Systems Inc INTRODUCTION Exosomes are small lipid membrane vesicles (80-120 nm) of endocytic origin generated
Medical Officer of Health Medical Director, Travel Health and Tropical Medicine Services Winnipeg Regional Health Authority
DNA Vaccines The Future of Travel Health Vaccines? Or Just a Bad Idea? Dr. Pierre J. Plourde Medical Officer of Health Medical Director, Travel Health and Tropical Medicine Services Winnipeg Regional Health
IN VIVO STUDIES ON VIRAL VIRULENCE
IN VIVO STUDIES ON VIRAL VIRULENCE M.Phil student: Emily TSUI Supervisor: Professor Paul K.S Chan Department of Microbiology, CUHK Date: 15th Dec, 2014 Viral Virulence Capacity of a virus to cause disease
Summary and Discussion antigen presentation
Summary and Discussion antigen presentation 247 248 Summary & Discussion Summary and discussion: antigen presentation For a cell to communicate information about its internal health and status to the immune
Acquired Immunity 2. - Vaccines & Immunological Memory - Wataru Ise. WPI Immunology Frontier Research Center (IFReC) Osaka University.
Acquired Immunity 2 - Vaccines & Immunological Memory - Wataru Ise WPI Immunology Frontier Research Center (IFReC) Osaka University Outline 1. What is vaccine (vaccination)? 2. What is immunological memory?
1 Virus-like nanoparticles. 2 Cationic carriers for gene delivery
This lecture highlights the strategies that have been evolved to use biomimetic materials as gene delivery vehicles such that they exhibit virus-like efficiency in delivery of the oligonucleotides. 1 Virus-like
Spleen. mlns. E Spleen 4.1. mlns. Spleen. mlns. Mock 17. Mock CD8 HIV-1 CD38 HLA-DR. Ki67. Spleen. Spleen. mlns. Cheng et al. Fig.
C D E F Mock 17 Mock 4.1 CD38 57 CD8 23.7 HLA-DR Ki67 G H I Cheng et al. Fig.S1 Supplementary Figure 1. persistent infection leads to human T cell depletion and hyper-immune activation. Humanized mice
Molecular Cell Biology 5068 In class Exam 1 October 2, Please print your name: Instructions:
Molecular Cell Biology 5068 In class Exam 1 October 2, 2012 Exam Number: Please print your name: Instructions: Please write only on these pages, in the spaces allotted and not on the back. Write your number
100 years of Influenza Pandemic and the prospects for new influenza vaccines
100 years of Influenza Pandemic and the prospects for new influenza vaccines Dr John McCauley Director, WHO Collaborating Centre for Reference and Research on influenza The Francis Crick Institute London
NANO 243/CENG 207 Course Use Only
L9. Drug Permeation Through Biological Barriers May 3, 2018 Lipids Lipid Self-Assemblies 1. Lipid and Lipid Membrane Phospholipid: an amphiphilic molecule with a hydrophilic head and 1~2 hydrophobic tails.
2 - Adaptive Immunity
2 - Adaptive Immunity The Division of the Immune System - Macrophages are in the tissues, neutrophils migrate through the blood stream - There s a release of a chemical signal which attracts all the cells
Primary Isolation and Cultivation of Viruses
Primary Isolation and Cultivation of Viruses Practical Medical Virology 450 MBIO 2017-18 01/10/2017 Amal Alghamdi Reham Alahmadi Dalia Alsrar 1 Diagnostic Virology Virus Isolation and Cultivation Viral
chapter 17: specific/adaptable defenses of the host: the immune response
chapter 17: specific/adaptable defenses of the host: the immune response defense against infection & illness body defenses innate/ non-specific adaptable/ specific epithelium, fever, inflammation, complement,
Supporting Information
Supporting Information Chan et al. 1.173/pnas.9654916 A Patient B Xenograft C * remaining feature of normal lymph node * * * D lymphocytes Infiltrating transitional carcinoma cells E Enlarged axillary
Dendritic Cells in T Cell Migration and Tolerance Induction. Dimitris Skokos The Rockefeller University
Dendritic Cells in T Cell Migration and Tolerance Induction Dimitris Skokos The Rockefeller University infection mature DCs an epithilium with dendritic cells Immature DCs steady state Regulatory T cells,
Vaccination with OVA-bound nanoparticles encapsulating IL-7 inhibits the growth of OVA-expressing E.G7 tumor cells in vivo
292 Vaccination with OVA-bound nanoparticles encapsulating IL-7 inhibits the growth of OVA-expressing E.G7 tumor cells in vivo HIROKO TOYOTA 1, NORIKO YANASE 1, TAKAYUKI YOSHIMOTO 2, MITSUNORI HARADA 3,
Suppl. Figure 1. T 3 induces autophagic flux in hepatic cells. (A) RFP-GFP-LC3 transfected HepG2/TRα cells were visualized and cells were quantified
Suppl. Figure 1. T 3 induces autophagic flux in hepatic cells. (A) RFP-GFP-LC3 transfected HepG2/TRα cells were visualized and cells were quantified for RFP-LC3 puncta (red dots) representing both autolysosomes
Nanomedicine & Drug Delivery Systems Group Research Institute for Medicines and Pharmaceutical Sciences (imed.ul)
Faculty of Pharmacy of the University of Lisbon Nanomedicine & Drug Delivery Systems Group Research Institute for Medicines and Pharmaceutical Sciences (imed.ul) Leishmania in southern Europe. (Duiardin,