«πμ æ» μ å À ªï Ë Û Ë Ú æ..-.. ÚııÛ Volume 30 Number 2 May-Aug Contents «Original article

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1 Antimicrobial activity of Thai medicinal plants (Murraya paniculata, Azadirachta indica var. Siamensis, Chromolaena odorata) against periodontopathic bacteria Shear bond strengths of Co-Cr alloys luted with two resin cements ª Ë π ª ß à æ Õ Õß π ÈÕøíπ ËÕ à Œ Õ å π Õß øíπ ª æ à ÈÕ àõ π àõߪ Õß å å ª ß ÿ æ μ π μ ÿ æ àõߪ Á π π È𪻠ªï Ë 5 ß π ß ÿß æ À π àπª Õ ß â ß μ Õß Õ Àß Õ Ë Àπà 𪻠À º μ ±å â æ ß π Õ æ μá øï

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3 πμ æ» μ å À Original article πμ À ªï Ë 30 à Ë 2, 2553 Antimicrobial activity of Thai medicinal plants (Murraya paniculata, Azadirachta indica var. Siamensis, Chromolaena odorata) against periodontopathic bacteria Pirasut Rodanant M.D.Sc. (Periodontics) Lecturer, Department of Hospital Dentistry, Faculty of Dentistry, Mahidol University Phorntip Sae-Lim M.Sc., Postgraduate student, Department of Chemistry, Faculty of Science, Ramkamhaeng University Apichart Suksamrarn Ph.D. (Organic Chemistry) Professor, Department of Chemistry, Faculty of Science, Ramkamhaeng University Jintakorn Kuvatanasuchati M.Sc. (Oral Microbiology) Associate professor, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University Correspondence author: Pirasut Rodanant Department of Hospital Dentistry, 6 Yothi Street, Rachathewi, Bangkok 10400, Thailand research grant: This research is supported by Research grant from Faculty of Dentistry, Mahidol University Fiscal year received: 13 July 2009 accepted: 24 May 2010 Abstract Objective: The purpose of this study was to evaluate the antimicrobial activity of three Thai medicinal plants on three periodontopathic bacteria. Materials and methods: Parts of Murraya paniculata, Azadirachta indica var. Siamensis and Chromolaena odorata were extracted using various organic solvents of different polarity; i.e. n-hexane, ethyl acetate, dichloromethane, chloroform and methanol.the agar diffusion technique was used to screen the crude extracts for their antimicrobial activity against Porphyromonas gingivalis (ATCC33277, FDC381), Prevotella intermedia (ATCC25611), and Aggrigatibactor actinomycetemcomitans (ATCC43718,Y4). Results: All extracts demonstrated growth-inhibition effect against all strains of tested bacteria but were different in the spectrum of inhibition. The ethyl acetate extracts of Murraya paniculata leaves and Murraya paniculata branches exhibited comparable activity to that of the standard drug chlorhexidine when tested against Porphyromonas gingivalis FDC 381. Conclusion: Crude extracts from leaves and branches of Murraya paniculata, leaves of Azadirachta indica var. Siamensis, and leaves of Chromolaena odorata using appropriate organic solvent demonstrated antimicrobial activity to Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus Actinomycetemcomitans. Key words: antimicrobial activity, Thai medicinal plants, periodontopathic bacteria, crude extracts Antimicrobial activity of Thai medicinal plants (Murraya paniculata, Azadirachta indica var. Siamensis, Chromolaena odorata) against periodontopathic bacteria Pirasut Rodanant Phorntip Sae-Lim Apichart Suksamrarn Jintakorn Kuvatanasuchati 63

4 πμ À ªï Ë 30 à Ë 2, 2553 πμ æ» μ å À ƒ Ïμâ π ÈÕ ÿ π å Õß ÿπ æ ( â,, Õ) μàõ ÈÕ àõ ª πμåõ æ»ÿ å Õ Õπ πμå M.D.Sc. (Periodontics) Õ å πμ ßæ πμ æ» μ å À À æ æ å à È M.Sc., π» À Ÿμ ª» μ å ÿ Æ ± μ Õ π å À Àß Õ μ ÿ Ph.D. (Organic Chemistry)» μ å» μ å À Àß πμ Ÿ π ÿ μ M.Sc. (Microbiology) Õß» μ å ÿ πμ æ» μ å ÿã ß å À àõ μ ÿª ß å: æ ËÕ» ª æ Õß À Õß â, Ëßμâπ â, Õ π Èß Õß ÈÕ àõ ª πμåõ π ÿõÿª å» : àπμà ßÊ Õß â (Murraya paniculata), (Azadirachta indica var. Siamensis) Õ (Chromolaena odorata) â Ÿ π â μ Õ π å Ë È Ë μ μà ß π â à Œ π, Õ Õ μμ, Õ π, Õ øõ å πõ π Ë â Õ ƒ Ïμâ π ÈÕ ÿ π åμàõ ÈÕæÕ å ø π ß (ATCC33277, FDC381), ÈÕæ Õ π Õ (ATCC25611) ÈÕ Õ μ Õ å Õ π μ π å (ATCC43718,Y4). Õ å øøî Ëπ º» : ÿπ æ ÿ π ߃ Ï π Èß μ μ Õß ÿ æ π ÿå μà μ μà ß πª æ Õß Èß Èßπ È À Õß â Ëßμâπ â Ë â Õ Õ μμ ªìπμ ߪ æ Ë â ß Õ å Œ π 2% π Èß Õß ÈÕæÕ å ø π ß æ π ÿå FDC 381 ÿª: À Õß â, Ëßμâπ â, Õ Ë âμ Õ π å Ë À π ߪ æ π Èß Õß ÈÕ àõ ª πμåõ æõ å ø π ß, æ - Õ π Õ Õ Õ å Õ π Á Õ π å À : ƒ Ïμâ π ÈÕ ÿ π å, ÿπ æ, ÈÕ àõ ª πμåõ, À 64 μ μàõ Ë : æ»ÿ å Õ Õπ πμå πμ ßæ πμ æ» μ å À À 6. μ ÿß æœ À àß ß π ÿπ:» π È â ÿπ ÿπõÿ Àπÿπ πμ æ» μ å À À ª ªïß ª 2551 π ËÕß: 13 Æ 2552 π Õ μ æ æå: 24 æƒ 2553 Antimicrobial activity of Thai medicinal plants (Murraya paniculata, Azadirachta indica var. Siamensis, Chromolaena odorata) against periodontopathic bacteria Pirasut Rodanant Phorntip Sae-Lim Apichart Suksamrarn Jintakorn Kuvatanasuchati

5 πμ æ» μ å À Original article πμ À ªï Ë 30 à Ë 2, 2553 Shear bond strengths of Co-Cr alloys luted with two resin cements Wanlapa Tantipokin D.D.S., Master of Prosthodontics candidate Department of Prosthodontics Somchai Urapepon D.D.S., Grad. Dip. in Clin. Sc. (Prosthodontics), Ph.D. Department of Prosthodontics Abstract Objective: The purpose of this study was to compare shear bond strengths of two resin cements: Maxcem Elite (containing GPDM) and Panavia F (containing MDP) to Co-Cr alloy. Materials and methods: 24 pairs of two sizes of casting Co-Cr alloy cylinders ( 15x1, 10x2 mm) were bonded together by two different resin cements (Maxcem Elite and Panavia F). After bonding, they were stored in 37 ÌC water for 24 hours. After that, half of the specimens of each group were thermal cycled in 4 ÌC and 60 ÌC water for 10,000 cycles. Shear bond strength test was performed using a universal testing machine at a crosshead speed of 0.5mm/min. Results: Both resin cements showed no significant difference in shear bond strength to Co-Cr alloy with or without thermal cycling conditions. Conclusions: resin cement containing GPDM monomer (Maxcem Elite) can achieve bonds to Co-Cr alloys comparable to resin cement containing MDP monomer (Panavia F). Key words: MDP, GPDM, resin cement, shear bond strength, Co-Cr alloy Correspondence author: Somchai Urapepon Department of Prosthodontics 6 Yothi Street, Rachathewi, Bangkok 10400, Thailand Tel: dtsor@mahidol.ac.th research grant: - received: 23 July 2009 accepted: 22 July 2010 Shear bond strengths of Co-Cr alloys luted with two resin cements Wanlapa Tantipokin Somchai Urapepon 73

6 πμ À ªï Ë 30 à Ë 2, 2553 πμ æ» μ å À ß ß Õπ Õß π πμå2 π μàõ À Õ Õ åμ- μ πμ π.., π» ª πμ ª å πμ æ» μ å À À Õÿ æ æ.., ª. ± μ ( πμ ª å), Ph.D. πμ ª å πμ æ» μ å À À àõ μ ÿª ß å: æ ËÕ» ß ß Õπ Õß π πμå 2 π Õ Á Á Õ Ëª Õ â π Õ å æ ÕÁ æ π å Õø ˪ Õ â π Õ å ÕÁ æ Ë μ À Õ Õ åμ- ÿõÿª å» : À Õ Õ åμ- Ÿª ß Õ 2 π Õ âπºà π»ÿπ å ß 15x1 μ âπºà π»ÿπ å ß 10x2 μ ππ 24 Ÿà π μ â â π â π πμå 2 π ( Á Á Õ æ π å Õø) à Èπμ Õ à ß πõà ß ÿ Õÿ À Ÿ Ë 37 Õß» π π 24 Ë ß À ß àπ Èπß π ËßÀπ Ëß Õß μà ÿà Õ å ß Ë 4 60 Õß» ππ 10,000 Õ π Èπμ Õ à ß Õ ß ß Õπ â ËÕß Õ Ë Á À 0.5 μ μàõπ º» : ß ß Õπ Õß π πμå Èß 2 π μàõ À Õ Õ åμ- à μ μà ß πõ à ß π Èß ª μ Õ å ß ÿª: π πμå Á Á Õ Ëª Õ â π Õ å æ ÕÁ æ π å Õø ˪ Õ â π Õ å ÕÁ æ Àâ μ À Õ Õ åμ- à μ μà ß π À : ÕÁ æ, æ ÕÁ, π πμå, ß ß Õπ, À Õ Õ åμ- 74 μ μàõ Ë : Õÿ æ æ πμ ª å πμ æ» μ å À À 6. μ ÿß æœ 10400» æ å: Õ å: dtsor@mahidol.ac.th À àß ß π ÿπ: - π ËÕß: 23 Æ 2552 π Õ μ æ æå: 22 Æ 2553 Shear bond strengths of Co-Cr alloys luted with two resin cements Wanlapa Tantipokin Somchai Urapepon

7 πμ æ» μ å À»ÿ ÿ ±π ÿ ª. Èπ Ÿß ( ÕÁπ Õπμå), Õ..( ÕÁπ Õπμå) πμ À μ ÕÁπ Õπμå πμ æ» μ å À À π å æ å».., ª. ± μ ( ÕÁπ Õπμå) ßæ Á æ π ß â μ Ï æ å À Õ Õ Õ μà ßÀ ÿ μ μàõ Ë :»ÿ ÿ ±π ÿ πμ À μ ÕÁπ Õπμå πμ æ» μ å À À 6. μ ÿß æœ 10400» æ å: : Õ å: dtsst@mahidol.ac.th À àß ß π ÿπ: - π ËÕß: 23 ÿ æ π å 2553 π Õ μ æ æå: 29 π 2553 πμ À ªï Ë 30 à Ë 2, 2553 ª Ë π ª ß à æ Õ Õß π ÈÕøíπ ËÕ à Œ Õ å π Õß øíπ àõ μ ÿª ß å: æ ËÕ» ª à æ Õ Õß π ÈÕøíπ Ë ß ª À ß à Œ Õ å 2 Õ Ëº πè Ëπ Á Ÿª π Õß øíπ ÿõÿª å» : âøíπ Ë Õπ ππ 45 Ë À ß μ μ øíπõõ μ Õß øíπ â Ÿ ˺ øíπμ ß ª øíπ Àâ À Õ π ÈÕøíπÀà ß Õß øíπ 1 μ Ë ß øíπ Ÿ Ë â π Àπâ Àâ À Õ π ÈÕøíπÀπ 1 μ Ë â π Èπ À Õ π ÈÕøíπÀπ 2 μ ªî ª øíπ â Ⱥ Èß Àπ Á 2 Èπ àßøíπ ªìπ 3 ÿà π øíπ ÿ ÿà ª à æ Õ Ë μâπ Ë Ÿ Ë â â ËÕß æ Õ ÿà ππ 5 Ë ªìπ ÿà ÿ àπè Ëπª» Õ ÕÕπ ππ 5 μ π Õß øíπ ÿà Ë 2 ππ 20 Ë ªíòπ Œ Õ å ˺ πè Ëπ â ª π Õß øíπ â π Ÿ ª ÿà Ë 3 ππ 20 Ë Õ μ À Õ â ª π Õß øíπ ªî ß â Õß øíπ π Õøíπ ÿ ÿà â Àπ 4 μ Á ªî π 2 Èπ à æ ß μ Ÿ Àπ πàπ Õß Œ Õ å π Õß øíπ π øíπ ÿ ÿà ª à πøõ øμ ø øõ å π Á â ËÕÿ À Ÿ 37 Õß» Èπ æ å âõ 100 π Èππ øíπ à æ Õ Õß π ÈÕøíπ π π Ë 1 ß 7 π Ë 14 º» : à æ Õ Õß π ÈÕøíπ π ÿà Œ Õ å ˺ πè Ëπ ÿà Õ μ à Ÿß à π Ë μâπ àõπ à Œ Õ å Ÿß à ÿà ÿ Õ à ß π (p<0.05) ÿà Œ Õ å ˺ πè Ëπ à æ Õ Õß π ÈÕøíπ Ÿß à ÿà Õ μ π ß ª Õ à ß π (p<0.05) Ë ß øíπ Ë π ÈÕøíπÀπ 1 μ 2 μ à æ Õ Ÿß à ª øíπõ à ß (p<0.05) Ë ß øíπ Ë π ÈÕøíπÀπ 1 μ à æ Õ Ÿß à π ÈÕøíπ ËÀπ 2 μ Õ à ß π (p<0.05) ÿª: Œ Õ å ˺ πè Ëπ Õ μ º Àâ à æ Õ Õß π ÈÕøíπ Ÿß ÈπÕ à ß π (p<0.05) à æ Õ Õß π ÈÕøíπ Ë Ÿß Èπ Œ Õ å ˺ πè Ëπ Ÿß à Ë Õ μ Õ à ß π (p<0.05) Ë ß øíπ à æ Õ Õß π ÈÕøíπ Ÿß à Ë ª øíπõ à ß π (p<0.05) à æ Õ π ÈÕøíπμ Àπàß ËÕ ŸàÀà ß Õß øíπ 1 μ à Ÿß à μ Àπàß ËÕ ŸàÀà ß Õß øíπ 2 μ Õ à ß π Ë π (p<0.05) À : à æ Õ Õß π ÈÕøíπ, Œ Õ å, Õ μ ª Ë π ª ß à æ Õ Õß π ÈÕøíπ ËÕ à Œ Õ å π Õß øíπ»ÿ ÿ ±π ÿ π å æ å» 81

8 πμ À ªï Ë 30 à Ë 2, 2553 πμ æ» μ å À Original article The ph changes of root dentine when dressing with calcium hydroxide Supachai Sutimuntanakul Grad. Dip. in Clin. Sc. (Endodontics), Diplomate Thai Board of Endodontics Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University. Charinthip Srisumran D.D.S., Grad. Dip. in Clin. Sc. (Endodontics) Queen Sirikit Hospital, Naval Medical Department, Satahip, Chonburi Thailand Correspondence author: Supachai Sutimuntanakul Department of Operative Dentistry and Endodontics,. 6 Yothi Street, Rachathewi, Bangkok 10400, Thailand Tel: Fax: dtsst@mahidol.ac.th Research grant: - received: 23 February 2010 accepted: 29 April 2010 Abstract Objective: This study was to compare the dentinal ph when dressing root canals with calcium hydroxide paste and Ultracal. Materials and Methods: Forty five extracted human single-rooted teeth were sectioned at crown level. Root canals were instrumented with step back technique. Three cavities were drilled on each root surface. One cavity was on the lingual surface at apical level and drilled to the depth that left a 1-mm dentin thickness from the root canal wall. The other two cavities were at the middle level. One cavity was on the facial surface until the dentine had a 1-mm thickness and the other cavity was on the lingual surface with 2 mm of dentine thickness. All roots apices were sealed with sticky wax and coated with two layers of nail varnish. Initial dentinal ph was measured from each drilled cavity by a calibrated microelectrode. Specimens were randomly divided into 3 groups. Group I, 5 roots served as control and root canals were filled with deionized water. Groups II and III, 20 roots in each group were filled with calcium hydroxide paste and Ultracal respectively. Coronal access in all groups were sealed with Cavit for 4 millimeters depth. Radiographs were taken to check the thouroughly filling of calcium hydroxide, then 2 layers of nail varnish was coated over the Cavit. All groups were stored in phosphate buffer saline at 37 ÌC and 100% humidity. The dentinal ph of all cavities were measured daily from 1 to 7 days and at 14 days. Results: Dentinal ph of each experimental group increased higher than the initial ph and the control group (p<0.05). The dentinal ph of the calcium hydroxide paste group was significantly higher than the Ultracal group at the middle level with 1 mm and 2 mm dentine thickness and apical level (p<0.05). The dentinal ph at the middle level with 1 mm and 2 mm dentine thickness were raised significantly higher than the apical level (p<0.05). At the middle level, the ph of 1 mm dentine thickness was significantly higher than that of 2 mm dentine thickness (p<0.05). Conclusion: The dentinal ph of calcium hydroxide group was significantly higher than Ultracal group (p<0.05). The dentinal ph at the middle level was raised more significantly than the apical level (p<0.05). The ph of dentine at 1 mm from root canal was significantly higher than that of 2 mm at the same time interval (p<0.05). Key words: dentinal ph, calcium hydroxide, Ultracal 82 ª Ë π ª ß à æ Õ Õß π ÈÕøíπ ËÕ à Œ Õ å π Õß øíπ»ÿ ÿ ±π ÿ π å æ å»

9 πμ æ» μ å À πμ À ªï Ë 30 à Ë 2, 2553 ª æ à ÈÕ àõ π àõߪ Õß å å ª ß Õ Ÿà π., ª. Èπ Ÿß ( ÕÁπ Õπμå) ÿà ß π πμ ßæ μ π æ π» å Ph.D. (Microbiology & Immunology) Õß» μ å ÿ πμ æ» μ å À À àõ μ ÿª ß å: æ ËÕ» ª ª æ Õß å å ª ß π à π Ëæ π Õß øíπ ÿõÿª å : μ å Ëߪ Õ â æ ø Õ π π π π å ª ß Ëߪ Õ â æ ø Õ π π ÁÕ π π ªØ π å Èß 3 π π âπ º π πõ μ àπ 1: 1: 1 μ ÕÁ æ π Õ æ æ π Õ º π πõ μ àπ 1:1 π Èπº ªØ π å Èß 2 Ÿμ ÕÁ æ πõ μ àπ 7:1 Õ ª æ à ÈÕ Õß å â Õ å øî Ëπ (Agar well diffusion technique) â Ëæ π Õß øíπ 7 π â à ÈÕ Ÿ π Õ Ÿ π ÈÕ Õ Õ ÈÕ ÕÁπ Õ Õ øï ÈÕ μ Áª μ Õ π å ÈÕ μ Õ ÈÕæÕ åøî π π ÈÕøî π Õμ º» : å å ª ß π ÕÁ æ à ÈÕ Èß 7 π â ËÕ ª π âπºà»ÿπ å ß Õßß Èß ÈÕ Àà ß Èß Õß æ à àμà ß πõ à ß π ß μ (p>.05) ÿª: å ª ß Ëª Õ â æ ø Õ π π ÁÕ π π â π μ ÈÕ π Õß øíπ â àπ å À : å, ÁÕ π, π Õß øíπ μ μàõ Ë : æ π» å ÿ πμ æ» μ å À À 6. μ ÿß æœ 10400» æ å: : Õ å: dtrss@mahidol.ac.th À àß ß π ÿπ: ÿπ àß ß π ßæ μ π ªïß ª 2553 π ËÕß: 17 π 2553 π Õ μ æ æå: 7 Æ 2553 ª æ à ÈÕ àõ π àõߪ Õß å å ª ß Õ Ÿà π æ π» å 91

10 πμ À ªï Ë 30 à Ë 2, 2553 Swaros Yuyuen DDS., Cert. in Endodontics Division of Dentistry, Taksin Hospital Ratchapin Srisatjaluk Ph.D. (Microbiology & Immunology) Associate professor, Department of Microbiology, πμ æ» μ å À Original article Antibacterial efficiency of 3Mix and modified 3Mix against oral pathogens Abstract Objective: To compare the antibacterial effectiveness of 3Mix and modified 3Mix antibiotics against the bacteria found in endodontic infections. Methods and Materials: 3Mix containing ciprofloxacin, metronidazole, and minocyclin and modified 3Mix containing ciprofloxacin, metronidazole, and doxycyclin were prepared by mixing the antibiotics with the macrogol and propylene glycol (MP). The antimicrobial effects of both 3Mix pastes were assessed by the agar well diffusion technique. The test bacteria were Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Streptococcus mutans, Lactobacillus casei, Porphyromonas gingivalis, and Fusobacterium nucleatum. Results: 3Mix and modified 3Mix inhibited growth of all test bacteria. When comparing the inhibition zones produced from both 3Mix antibiotic paste, no significant difference was found, p>.05. Conclusion: Modified 3Mix containing ciprofloxacin, metronidazole, and doxycyclin can use for endodontic medicament. Key words: 3Mix, doxycyclin, endodontic bacteria 92 Correspondence author: Ratchapin Srisatjaluk Department of Microbiology, 6 Yothi Street, Rachathewi, Bangkok 10400, Thailand Tel: Fax: dtrss@mahidol.ac.th Research grant: Taksin Hospital received: 17 March 2010 accepted: 7 July 2010 ª æ à ÈÕ àõ π àõߪ Õß å å ª ß Õ Ÿà π æ π» å

11 πμ æ» μ å À πμ À ªï Ë 30 à Ë 2, 2553 ÿ æ μ π μ ÿ æ àõߪ Á π π È𪻠ªï Ë 5 ß π ß ÿß æ À π ÿμ æ Ï â..,.. Õß πμ ÿ π Õπ ÿß æ À π μ μàõ Ë : ÿμ æ Ï â Õß πμ ÿ π Õπ ÿß æ À π 123. μ μ μ π ß ÿß æœ 10400» æ å: » æ å Õ Õ: : Õ å: cphokaew@hotmail.com À àß ß π ÿπ: ÿπ àπμ π ËÕß: 25 惻 π 2553 π Õ μ æ æå: 21 Æ 2553 àõ μ ÿª ß å: æ ËÕª π ÿ â π Õߺ μàõ ªØ μ ª π π ËÕß ªí À π àõߪ Õßπ π È𪻠ªï Ë 5 ß πª» ß ÿß æ À π ÿõÿª å» :» ß μ ß π π Àà ß Õπ ßÀ ß π π æ.».2552 åπ π È𠪻 ªï Ë 5 Õ ÿ10-12 ªï ππ 481 π â ËÕß Õ ÿ æ μ π μ ÿ æ àõߪ À Á Õ Child-OIDP π Èπμ àõߪ π øíπºÿ â ±å øíπºÿ ÕßÕß å Õπ (WHO criteria 1997) º» : ÿ øíπºÿ πøíπ Õß ÿà μ Õ à ß à âõ 43 ÿà μ Õ à ß à Ë øíπºÿ Õπ Õÿ à 0.88 ËμàÕ π(±1.30) ÿ Õß øíπºÿ à Ë øíπºÿ Õπ Õÿ Õß ÿà μ Õ à ß Àà ß æ» æ»à ß μ μà ß πõ à ß π ß μ (p<0.05) ÿà μ Õ à ß âõ 55.1 â º π ªØ μ ª π ªí À π àõߪ π 3 Õπ ˺à π ª ËßÀπ Ëß( âõ 30.8) ÕߺŸâ â º ÿà â º æ ß 1 ÿ Õß º π Á æ» À ß à μ μà ß π à Ë ππº à 3.4 ±6.1 ππ âõ 61.8 Õß ÿà μ Õ à ß â º π Á πâõ ß Á πâõ ª π 8 ª πõ À ªìπ Ë â º Ë ÿ Õ âõ 38.3 Õ øíπ Õ ª øíπ øíππè π À À ÿ ªìπ Àμÿ Ë Àâ º μ Ÿâ Á Ë ÿ μ Õ âõ 45.7 âõ 26.0 âõ 23.0 μ ÿª: ÿ â Õߺ μàõ ªØ μ ª π Ë Àμÿ ªí À àõߪ Ëæ π Á ÿà π ÈÕ Ÿà π ª π ß (55.1%) àπ À à (61.8%) â Õߺ π πâõ πâõ àπ π Õߺ πâõ ( ππ 3.4 (±6.1) ππ μá 100) ª πõ À ªìπ Ë â º Ë ÿ Àμÿμ Ÿâ Ë Á ÿ Ë ÿ Õ øíπ ª øíπ øíπ πè π À À ÿ μ À : ÿ æ μ π μ ÿ æ àõߪ, ÿ æ μ, Á π π ÿ æ μ π μ ÿ æ àõߪ Á π π È𪻠ªï Ë 5 ß π ß ÿß æ À π ÿμ æ Ï â 103

12 πμ À ªï Ë 30 à Ë 2, 2553 πμ æ» μ å À Original article Oral health-related quality of life of pratomsuksa 5 students in schools under the Jurisdiction of Bangkok Metropolitan Administration Chutima Phokaew D.D.S., M.P.H. Dental Health Division, Health Department, Bangkok Metropolitan Administration Correspondence author: Chutima Phokaew Dental Health Division, Health Department, Bangkok Metropolitan Administration 123 Mitr maitri Rd, Dindaeng district, Bangkok 10400, Thailand. Tel: Mobile phone: Fax: cphokaew@hotmail.com Research grant: Personal fund received: 25 November 2009 accepted: 21 July 2010 Abstract Objective: To assess the prevalence, intensity and extent of the impacts caused by oral problems on daily performances of Pratomsuksa 5 students in schools under the jurisdiction of Bangkok Metropolitan Administration, Bangkok, Thailand. Materials & methods: Cross-sectional study was performed in August - September Four hundred and eighty-one schoolchildren aged years were interviewed about oral impacts on daily performance during the past 3 months, using the Child Oral Impacts on Daily Performances Index (Child-OIDP), and then were examined for dental caries status by WHO Oral Health Surveys, 4th ed, 1997 criteria. Results: The prevalence of dental caries was 43 %. The mean DMFT score was 0.88 (±1.30) There were statistically significant differences in prevalence of dental caries and the mean DMFT scores by gender (p<0.05). Fifty-five percentages of children had one or more impacts, with 30.8% reported only 1 affected performance. There was no difference in prevalence of impacts between girls and boys. Mean impact score was 3.4 (±6.1). More than half (61.8 %) of those with impacts were affected in very little or little intensity. Among the eight performances, eating was the most frequently reported oral impact (38.3%). Sensitive tooth, toothache and an exfoliating primary tooth were mainly perceived as the causes of impacts (45.7%, 26.0% and 23.0% respectively). Conclusion: The prevalence and intensity of oral impact on daily performance of these children were medium. Fifty-five percentages of the interviewed children reported oral impacts on daily performances during the last three months. Most of those with impacts (61.8%) reported impacts of very little or little intensity. The overall impact score was low (score 3.4(± 6.1) from 100). Eating was the most commonly affected performance. The main oral conditions that caused impacts were sensitive tooth, toothache and an exfoliating primary tooth. Key words: oral health related quality of life, quality of life, schoolchildren 104 ÿ æ μ π μ ÿ æ àõߪ Á π π È𪻠ªï Ë 5 ß π ß ÿß æ À π ÿμ æ Ï â

13 πμ æ» μ å À πμ À ªï Ë 30 à Ë 2, 2553 àπª Õ ß â ß μ Õß Õ Àß Õ Ë Àπà 𪻠À º μ ±å»ÿæÿ ß.. ( πμ ª å), Ph.D. (Materials Science and Engineering) πμ ª å πμ æ» μ å À À Õ μπå ß å Ë.. ( πμ ª å), Ph.D. (Dental Materials Science) πμ ª å πμ æ» μ å À À ÿ μ È æß»å M.Phil. (Dental materials), Õ..( πμ ª å) πμ ª å πμ æ» μ å À À æ æ å»ÿ å».., ª. ± μ ( πμ ª å) ÿà ß π πμ ÿ π ß π ÿ ßÀ ÿ π å μ μàõ Ë : Õ μπå ß å Ë πμ ª å πμ æ» μ å À À 6. μ ÿß æœ 10400» æ å: μàõ 6441 Õ å: dtawl@mahidol.ac.th À àß ß π ÿπ: πμ æ» μ å À À π ËÕß: 13 Æ 2552 π Õ μ æ æå: 7 Æ 2553 àõ μ ÿª ß å: π È μ ÿª ß å æ ËÕ» ß â ß àπª Õ μ ËÕ ªï π ß ß Õß Õ Àß Õ Ë Àπà 𠪻 ππ 6 º μ ±å ÿõÿª å Õ : Õ Àß Õ Ë» â à μ μ åæÿμ å Õ μ æ π» Õ ππ âπ â Ë ªìπ àπª Õ â âõß ÿ»πå ß μë» Õß âπ Õ Àß Õ π âπºà»ÿπ å ß Õß âπ Õ Àß Õ â âõß ÿ»πåõ μ Õπ àõß Àå π Õß âπ μ Õ Õß π ß π μ π Õÿμ À 121 à Ë 15:1981 AATCC 20:2004» μ Õß âπ Õ Àß Õ â ª Ë π ª ß Õß âπºà»ÿπ å ß Õß âπ Õ â âõß ÿ»πå àõπ À ß àπè π º μ Õß Õ Àß Õ ªìπ âõ Àå ª ª π Ë π 0.05» π ß ß Õß Õ Àß Õ π æ Àâß æ ªï â ËÕß Õ Àå π ß ß â Àå ª ª π Ë π 0.05 º» : æ à Õ Àß Õ àπ À à Õ ªìπ ªï âπ ΩÑ ªìπ àπª Õ âπ Õ Ë μ À ß à Ÿß ÿ à Ë π ß ß Ÿß ÿ Õ μ Õ å 2 μ âõ 15.1 π ß ß 84.8 π μ π π æ Àâß æ ªï à Ë π ß ß Õß âπ Õ μ Õ å 2 μ åæÿμ Õ å 1 π Õ å 0 å Õ å 9 μ μà ß πõ à ß π ÿª: μ ËÕ ªï ÈπÕ Ÿà π âπ âπºà»ÿπ å ß π ß ß Õß âπ Õ Àß Õ ÈπÕ Ÿà π Õß âπ π Õß âπºà»ÿπ å ß Õß âπ Õ À : Õ Àß Õ, àπª Õ, μ ËÕ ªï, π ß ß àπª Õ ß â ß μ Õß Õ Àß Õ Ë Àπà 𪻠À º μ ±å»ÿæÿ ß Õ μπå ß å Ë ÿ μ È æß»å æ æ å»ÿ å» 117

14 πμ À ªï Ë 30 à Ë 2, 2553 πμ æ» μ å À Original article Composition, structure and properties of six gingival retraction cords commercially available in Thailand. Kallaya Suputtamongkol M.Sc. (Prosthodontics), Ph.D. (Materials Science and Engineering) Department of Prosthodontics, Amornrat Wonglamsam M.Sc. (Prosthodontics), Ph.D. (Dental Materials Science) Department of Prosthodontics, Chatcharee Suchatlampong M.Phil. (Dental materials), Dip. Thai Board (Prosthodontics) Department of Prosthodontics, Porntip Sukornvetsiri D.D.S., Grad. Dip in Clinical Science (Prosthodontics) Dental Public Health Division, Surin Provincial Public Health Office Correspondence author: Amonrat Wonglamsam Department of Prosthodontics, 6 Yothi Street, Rachathewi, Bangkok 10400, Thailand Tel: ext dtawl@mahidol.ac.th Research grant: Faculty of Dentistry, Mahidol University received: 13 July 2009 accepted: 7 July 2010 Abstract Objectives: The objectives of this study were to determine the structure, composition, percentage of expansion after immersion in distilled water, and tensile strength of six gingival retraction cords commercially available in Thailand. Materials and methods: Six gingival retraction cords used in this study were Roeko Retracto Roeko Stayput Sil-trax Ultrapak Gingibraid and Voco Traco. Pattern of weaving and number of threads were observed using light microscope. Characteristics of cord and cord diameter were observed using scanning electron microscopy. Types of fiber were identified using TIS 121 book 15:1981 and AATCC 20:2004 techniques. The amount of expansion of gingival retraction cords were determined by measuring the diameter before and after immersion cord. The difference between means diameter were calculated as expansion percentage. ANOVA was used for analyzing the percentage expansion at 0.05 significance level. Tensile testing was performed using a universal testing machine at a cross-head speed of 100 mm/min. ANOVA was used for analyzing the tensile strength at 0.05 significance level. Results: Most gingival retraction cords used in this study were braided, composed of cotton fiber. Roeko Retracto No.2 had the highest expansion after immersion (15.1 %) and the highest means tensile strength (84.8 N). In dry and wet condition, means tensile strength of Roeko Retracto No.2, Roeko Stay-put No.1, Gingibraid No.0 and Sil-trax No.9 were significantly different. Conclusions: The amount of cord expansion depended on the type of fibers and diameter. Mean tensile strength of gingival retraction cord was influenced by the type of fibers and diameter. Keywords: gingival retraction cord, composition, expansion after immersion, tensile strength 118 àπª Õ ß â ß μ Õß Õ Àß Õ Ë Àπà 𪻠À º μ ±å»ÿæÿ ß Õ μπå ß å Ë ÿ μ È æß»å æ æ å»ÿ å»

15 πμ æ» μ å À ª»πå πμ À ªï Ë 30 à Ë 2, 2553 â æ ß π Õ æ μá øï π μ μ Àπàßøíπ π: ß πºÿâªé 3 ª æ» ß å.., ª. ± μ ( ß àõߪ Á ø ) ß πμ æ» μ å À À å ß æ.., ª. ± μ ( ß àõߪ Á ø ) ß πμ æ» μ å À À æ æ πå øóòõß æ å.., Ph.D. (Dental Science) πμ ßæ πμ æ» μ å À À» å ß»å μ.., Õ.. (»» μ å àõߪ Á ø )»» μ å πμ æ» μ å À À àõ øíπ π ªìπ º ª μ π â π æ π ππøíπ π øíπ àõàπàõøíπ øíππ ÈπÕ ΩíßÕ Ÿà π Ÿ À Õ Èπ π àõߪ Àâ ÀÁπ ß àπà Õ ß Èß ÀÁπ μá Ëøíπ øíπ π ËΩíßÕ Ÿà π Ÿ μ æ â æ ß Ëß πªí ÿ π π à æ ß π Õ æ μá øï à π Õ μ Àπàßøíπ π â à π Ë ª π È ªìπ ß πºÿâªé Ëæ øíπ π ππ 3 π ºŸâªÉ Ë 1 æ øíπ π Èπ à â 2 Ë Õ Ÿà π Õß æ πª À ßøíπμ ºŸâªÉ Ë 2 æ øíπ πõ Ÿà â π â Õßøíπ Ë Ë Õß à ß â πºÿâªé Ë 3 æ øíπ πõ Ÿà Õßøíπ Ë ËÀπ Ëß π À : øíπ π, æ ß àπμ Õ» Õ æ μõ å, π Õ æ μá - øï μ μàõ Ë : ª æ» ß å ß πμ æ» μ å À À 6. μ ÿß æœ 10400» æ å: Õ å: dtpsm@mahidol.ac.th À àß ß π ÿπ: - π ËÕß: 3 π 2552 π Õ μ æ æå: 11 π 2553 â æ ß π Õ æ μá øï π μ μ Àπàßøíπ π: ß πºÿâªé 3 ª æ» ß å å ß æ æ æ πå øóòõß æ å» å ß»å μ 129

16 πμ À ªï Ë 30 à Ë 2, 2553 Preeyaporn Srimawong D.D.S., Grad.Dip.in Clin.Sc. (Oral and Maxillofacial Radiology) Department of Radiology,. Khumpee Songkampol D.D.S., Grad.Dip.in Clin.Sc. (Oral and Maxillofacial Radiology) Department of Radiology,. Pornpoj Fuangtharnthip D.D.S., Ph.D. (Dental Science) Department of Hospital Dentistry,. Natthamet Wongsirichat D.D.S., Diplomate Thai Board of Oral and Maxillofacial Surgery Department of Surgery,. Application of cone beam computed tomography for supernumerary tooth Abstract Supernumerary tooth is a developmental anomaly in number during the formation of the dental germ. These teeth may remain embedded in the alveolar bone or erupt into the oral cavity. When it embedded, the diagnosis is performed through conventional radiographs. In the present, it has new technique called cone beam computed tomography. These modalities shows the superiority of images compared with conventional radiographs. This paper will report of three cases of supernumerary teeth. The first case shows two embedded supernumerary teeth located in nasopalatine canal. The second case shows paramolar located in buccal side of lower left second molar. The third case shows supernumerary tooth located in furcation of upper right first molar. Key words: supernumerary tooth, computed tomography, cone beam computed tomography πμ æ» μ å À Review article 130 Correspondence author: Preeyaporn Srimawong Department of Radiology, 6 Yothi Street, Rachathewi, Bangkok 10400, Thailand Tel: dtpsm@mahidol.ac.th research grant: received: 3 December 2009 accepted: 11 March 2010 â æ ß π Õ æ μá øï π μ μ Àπàßøíπ π: ß πºÿâªé 3 ª æ» ß å å ß æ æ æ πå øóòõß æ å» å ß»å μ

17 πμ æ» μ å À Mahidol Dental Journal ªï Ë Ûapple Ë Ú æ..-.. ÚııÛ Volume 30 Number 2 May-Aug ƒ Ïμâ π ÈÕ ÿ π å Õß ÿπ æ ( â,, Õ) μàõ ÈÕ àõ ª πμåõ æ»ÿ å Õ Õπ πμå æ æ å à È Õ μ ÿ πμ Ÿ π ÿ μ ß ß Õπ Õß π πμå 2 π μàõ À Õ Õ åμ- μ πμ π Õÿ æ æ ª Ë π ª ß à æ Õ Õß π ÈÕøíπ ËÕ à Œ Õ å π Õß øíπ»ÿ ÿ ±π ÿ π å æ å» ª æ à ÈÕ àõ π àõߪ Õß å å ª ß Õ Ÿà π æ π» å ÿ æ μ π μ ÿ æ àõߪ Á π π È𪻠ªï Ë 5 ß π ß ÿß æ À π ÿμ æ Ï â àπª Õ ß â ß μ Õß Õ Àß Õ Ë Àπà 𪻠À º μ ±å»ÿæÿ ß Õ μπå ß å Ë ÿ μ È æß»å æ æ å»ÿ å» Original article Contents Antimicrobial activity of Thai medicinal plants (Murraya paniculata, Azadirachta indica var. Siamensis, Chromolaena odorata) against periodontopathic bacteria Pirasut Rodanant Phorntip Sae-Lim Apichart Suksamrarn Jintakorn Kuvatanasuchati Shear bond strengths of Co-Cr alloys luted with two resin cements Wanlapa Tantipokin Somchai Urapepon The ph changes of root dentine when dressing with calcium hydroxide Supachai Sutimuntanakul Charinthip Srisumran Antibacterial efficiency of 3Mix and modified 3Mix against oral pathogens Swaros Yuyuen Ratchapin Srisatjaluk Oral health-related quality of life of pratomsuksa 5 students in schools under the Jurisdiction of Bangkok Metropolitan Administration Chutima Phokaew Composition, structure and properties of six gingival retraction cords commercially available in Thailand. Kallaya Suputtamongkol Amornrat Wonglamsam Chatcharee Suchatlampong Porntip Sukornvetsiri ª»πå â æ ß π Õ æ μá øï π μ μ Àπàßøíπ π: ß πºÿâªé 3 ª æ» ß å å ß æ æ æ πå øóòõß æ å» å ß»å μ 129 Review article Application of cone beam computed tomography for supernumerary tooth Preeyaporn Srimawong Khumpee Songkampol Pornpoj Fuangtharnthip Natthamet Wongsirichat