Osteochondral Transplant System
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1 Osteochondral Transplant System Rodrigo Umanzor (Team Leader) Nick Zacharias (BWIG & BSAC) Bilin Loi (BPAG) Eduardo Enriquez (Communicator) Client: Dr. Brian Walczak, DO Advisor: Dr. Kris Saha, PhD February 17, 2017
2 Overview Client Overview Problem Statement Background on Procedure Current Designs Design Ideas Design Matrix Future Work Image Courtesy of: Osteochondral_defect
3 Client: Dr. Brian Walczak, DO Faculty, UW-Madison School of Medicine and Public Health Specialties: Orthopedic Surgery Pediatric Sports Medicine Knee Arthroscopy Walczak_Brian_DO.jpg
4 Problem Statement Osteochondral transplants are commonly used to correct defects in cartilage and bone tissue 20-25% chance of failure (Chahal, J, et al, 2013) Our Role: Create a new system that reduces the forces applied to cartilage layer during insertion Increase chondrocyte viability to decrease failure rate of procedure Figure 1: Graft recipient site (above) and inserted graft (below) 4 Images Courtesy of: S. Akhavan, A. Miniaci, M. T. Provencher, C. B. Dewing, A. G. McNickle, A. B. Yanke, an d B. J. Cole, Cartilage Repair an d Replacemen t: From Osteochon dral Autograft Tran sfer to Allograft, in SURGICAL TREATMENT OF THE ARTHRITIC KNEE: ALTERNATIVES TO TKA, pp
5 Product Design Specifications (PDS) Achieve more than 70% viability impaction forces < 165 N during implantation (Walczak, et al, 2016) Graft must exhibit proper integration postoperatively Tools used in procedure should be capable of operating on bone Range of 5mm-20mm diameter and at least 10 mm depth for damage repair Materials should be sterilizable and comply with FDA regulations
6 Current Clinical Procedure5 Proposed Design Procedure Reference: ALLOGRAFT CARTILAGE TRANSPLANT SURGICAL TECHNIQUE, MTF Sports Medicine. [Online]. Available: [Accessed: 16-Feb-2017].
7 Fluorescent Microscopy Stain Incubate Cryofreeze and Section thinly Image Figure 2: Fluorescent Microscope 7 Image Courtesy of
8 Flow Cytometry Digest using collagenase Stain Cells Fix Run through flow cytometer obtain live/dead numbers Figure 4: Flow Cytometer 8 Image Courtesy of
9 Confocal Microscopy Stain Incubate Fix and Section Image Multiple layers Figure 5: Confocal Microscope 9 Image Courtesy of
10 Design Matrix Criteria Fluorescent Microscopy Flow Cytometry Confocal Microscopy Accuracy (35) (3/5) 21 (4/5) 28 (5/5) 35 Cost (30) (5/5) 30 (1/5) 6 (4/5) 24 Ease of Use (20) (5/5) 20 (2/5) 8 (3/5) 12 Tissue Section Prep (10) (3/5) 6 (5/5) 10 (4/5) 8 Procedure Length (5) (3/5) 3 (2/5) 2 (5/5) 5 Total
11 Current Progress From Dec Figure 1: Threaded Bone plug Figure 2: Recipient holes 400 um Figure 3: Live dead staining. FITC (Live) TRITC (Dead)
12 Future Work Fabricate a guide to accurately extract a plug Thread the plug Test compatibility with threaded recipient hole Acknowledgments Dr. Saha Dr. Walczak
13 References 1. Chahal, J, et al. (2013). Outcomes of Osteochondral Allograft Transplantation in the Knee. Arthroscopy: The Journal of Arthroscopic & Related Surgery, 29(3), doi: /j.arthro J. L. Cook et al., "Importance of Donor Chondrocyte Viability for Osteochondral Allografts," American Journal of Sports Medicine, vol. 44, no. 5, pp , May =http%3a%2f%2fcartilage.org%2fpatient%2fabout-cartilage%2fcartilagerepair%2fallograft%2f&psig=afqjcnhid7ilhaee07d8el5kh4nshnju3a&ust= S. Akhavan, A. Miniaci, M. T. Provencher, C. B. Dewing, A. G. McNickle, A. B. Yanke, and B. J. Cole, Cartilage Repair and Replacement: From Osteochondral Autograft Transfer to Allograft, in SURGICAL TREATMENT OF THE ARTHRITIC KNEE: ALTERNATIVES TO TKA, pp S. L. Sherman, J. Garrity, K. Bauer, J. Cook, J. Stannard, and W. Bugbee, "Fresh Osteochondral Allograft Transplantation for the Knee: Current Concepts (vol 22, pg 121, 2014)," Journal of the American Academy of Orthopaedic Surgeons, vol. 22, no. 3, pp , Mar ALLOGRAFT CARTILAGE TRANSPLANT SURGICAL TECHNIQUE, MTF Sports Medicine. [Online]. Available: [Accessed: 16-Feb-2017]. 7. OLYMPUS BX51 FLUORESCENCE MICROSCOPE, Spach Optics Inc., [Online]. Available: [Accessed: 16-Feb-2017]. 8. Filters for Flow Cytometry, IDEX Health & Science LLC., [Online]. Available: [Accessed: 17-Feb-2017]. 9. Immunohistochemical Microscopy, Sino Biological Inc., [Online]. Available: [Accessed: 17-Feb-2017].
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