Trigeminal high-frequency stimulation produces short- and long-term modification of reflex blink gain

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1 J Neurophysiol : , 24. First published November 27, 23; doi:.52/jn Trigeminal high-frequency stimulation produces short- and long-term modification of reflex blink gain Michael Ryan, Jaime Kaminer, 2 Patricia Enmore, 4 and Craig Evinger,3 Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, New York; 2 Department of Psychology, Stony Brook University, Stony Brook, New York; 3 SUNY Eye Institute, Stony Brook, New York; and 4 Program in Neuroscience, Stony Brook University, Stony Brook, New York Submitted 7 September 23; accepted in final form 22 November 23 Ryan M, Kaminer J, Enmore P, Evinger C. Trigeminal highfrequency stimulation produces short- and long-term modification of reflex blink gain. J Neurophysiol : , 24. First published November 27, 23; doi:.52/jn Reflex blinks provide a model system for investigating motor learning in normal and pathological states. We investigated whether high-frequency stimulation (HFS) of the supraorbital branch of the trigeminal nerve before the R2 blink component (HFS-B) decreases reflex blink gain in alert rats. As with humans (Mao JB, Evinger C. J Neurosci 2: RC5, 2), HFS-B significantly reduced blink size in the first hour after treatment for rats. Repeated days of HFS-B treatment produced long-term depression of blink circuits. Blink gain decreased exponentially across days, indicating a long-term depression of blink circuits. Additionally, the HFS-B protocol became more effective at depressing blink amplitude across days of treatment. This depression was not habituation, because neither long- nor short-term blink changes occurred when HFS was presented after the R2. To investigate whether gain modifications produced by HFS-B involved cerebellar networks, we trained rats in a delay eyelid conditioning paradigm using HFS-B as the unconditioned stimulus and a tone as the conditioned stimulus. As HFS-B depresses blink circuits and delay conditioning enhances blink circuit activity, occlusion should occur if they share neural networks. Rats acquiring robust eyelid conditioning did not exhibit decreases in blink gain, whereas rats developing low levels of eyelid conditioning exhibited weak, short-term reductions in blink gain. These results suggested that delay eyelid conditioning and long-term HFS-B utilize some of the same cerebellar circuits. The ability of repeated HFS-B treatment to depress trigeminal blink circuit activity long term implied that it may be a useful protocol to reduce hyperexcitable blink circuits that underlie diseases like benign essential blepharospasm. blepharospasm; cerebellum; gain modification; motor learning; trigeminal nerve Address for reprint requests and other correspondence: C. Evinger, Dept. of Neurobiology and Behavior, Stony Brook Univ., Stony Brook, NY ( leslie.evinger@stonybrook.edu). MAMMALS SHARE a common motor control plan for trigeminal reflex blinks (Basso et al. 993, 996; Basso and Evinger 996; Gnadt et al. 997) and exhibit nearly identical patterns of muscle activity for all other types of blinking (Aramideh et al. 994; Evinger et al. 984, 99; Evinger and Manning 993; Kaminer et al. 2). These homologies make trigeminal reflex blinks an ideal model system for investigating motor learning and its relationship with movement disorders. For example, pathological reductions in lid motility and eye irritation initiate motor learning in trigeminal blink circuits (Evinger and Manning 988; Kassem and Evinger 26; Schicatano et al. 22) and eyelid dystonias appear to be exaggerations of this normally adaptive motor learning (Evinger et al. 22; Hallett et al. 28). It is possible to investigate these interactions between motor learning and disease states with high-frequency stimulation (HFS) of the supraorbital branch of the trigeminal nerve (SO) to create a short-term modification of reflex blink gain (Mao and Evinger 2). The HFS paradigm identifies motor learning abnormalities in Parkinson s disease, dystonia, and Tourette syndrome (Battaglia et al. 26; Crupi et al. 28; Quartarone et al. 26; Suppa et al. 2). Determining how these neurological disorders disrupt HFS gain modifications requires identification of the neural bases of this form of motor learning. Short-term HFS-induced modifications occur in trigeminal blink circuits only on the side of the brain receiving HFS. For example, after HFS treatment of the left SO to increase blink gain, stimulating the left SO evokes larger than normal blinks whereas right SO stimulation elicits normal-amplitude blinks. This pattern is analogous to other blink adaptations initiated by reduced eyelid motility (Cossu et al. 999; Evinger et al. 989; Evinger and Manning 988; Kassem and Evinger 26; Manca et al. 2; Schicatano et al. 22; VanderWerf et al. 27). It is unknown, however, whether HFS treatment reproduces the long-term changes in blink gain that occur with reductions in lid motility with facial palsy (Abell et al. 998; Manca et al. 2; VanderWerf et al. 27). We address the long-term effects by examining the consequence of multiple days of HFS treatment on blink reflex gain. Given the role of the cerebellum in gain modifications of blinking and other motor systems (Blazquez et al. 24; Boyden et al. 24; Evinger et al. 989; Hopp and Fuchs 24; Pellegrini and Evinger 997; Raymond et al. 996; Scudder and McGee 23; Soetedjo et al. 28), we delineate some of the critical neural circuits in HFS blink modification by combining HFS treatment designed to depress blink amplitude with cerebellum-dependent delay eyelid conditioning that increases blink circuit activity (Christian and Thompson 23; De Zeeuw and Yeo 25; McCormick and Thompson 984; Thompson 25; Topka et al. 993). We posit that if delay conditioning and HFS-induced modifications utilize overlapping cerebellar circuits, then some occlusion will occur between the two forms of learning. MATERIALS AND METHODS Experiments were performed on 2 male Sprague-Dawley rats (75 55 g) maintained on a reversed 2:2-h light-dark cycle and fed ad libitum. Data were collected during the rats subjective night. All experiments received approval by the Stony Brook University Institutional Animal Care and Use Committee and complied with all /4 Copyright 24 the American Physiological Society Downloaded from by ${individualuser.givennames} ${individualuser.surname} ( ) on February 9, 28. Copyright 24 American Physiological Society. All rights reserved.

2 MODIFYING REFLEX BLINK GAIN 889 federal, state, and university regulations regarding the use of animals in research. Surgery. Under general anesthesia (9 mg/kg ketamine and mg/kg xylazine), rats were prepared for chronic recording of the orbicularis oculi (OO) EMG (OOemg) and SO stimulation (Dauvergne and Evinger 27; Evinger et al. 993). To record the OOemg, a pair of Teflon-coated wires (.3-in. diameter bare,.55 in. coated; no. 79, A-M Systems, Everett, WA) with mm exposed at the tip were implanted in the OO muscle below the lateral canthus. To stimulate the SO nerve, a nerve cuff containing a pair of stainless steel wires with the insulation removed (.3-in. diameter bare,.55 in. coated; no. 79, A-M Systems) encased in Teflon tubing (-mm diameter; no. 633, Small Parts, Miami, FL) was placed around the SO branch of the trigeminal nerve. Wires were led subcutaneously to a connector embedded in a dental acrylic platform on the skull. The platform was attached to the skull by four stainless steel screws. A silver wire connected to one of the stainless steel screws served as the ground. Rats received an analgesic (ketorolac, 7 mg/kg) for at least 24 h after the surgery. Rats were alert and eating within 24 h of the surgery. The experiments began at least wk after the surgery. Stimulation paradigm. The SO stimulus in all experiments was relative to the minimum current at which a unipolar - s stimulus reliably elicited the R component of a reflex blink, threshold (T). This current was determined at the beginning of each day for each rat A R SO Alone R2 E Pre 2 trials and was held constant throughout that day s experiment. Across all subjects and days tested, the range of threshold currents was 9 A, with a median of 25 A. For each rat, threshold varied little across days. The range of coefficients of variation for thresholds across all rats was.25.35, with a mean of.3.3. All data were collected at twice threshold (2T), a stimulus intensity that evoked a strong R response and an inconsistent and small R2 component in rats (Fig. A) (Basso et al. 993; Dauvergne and Evinger 27; Evinger et al. 993). The HFS train was five 2T SO stimuli presented at 4 Hz. Each day s data collection consisted of five blocks: ) before treatment, 2) treatment condition, 3) immediately after treatment, 4) 3 min after treatment, and 5) 6 min after treatment (Fig. E). Preand posttreatment blocks were the same for all experiments. In these blocks, rats received 2 trials of paired 2T SO stimuli with an interstimulus interval of ms. Thus two blinks were evoked in each nontreatment trial. The first evoked blink was called the condition blink, and the second blink was termed the test blink. The intertrial interval for all data blocks varied pseudorandomly over the range of 2 5 s. Each rat received at least days of a given treatment. Treatment conditions. One group (n ) underwent a blink motor learning paradigm to depress blink amplitude (Mao and Evinger 2). As with the human study, rats in this treatment group received 6 trials of high-frequency SO stimulation that occurred during the R but before the R2 component of the blink (HFS-B). Each treatment ms B HFS-B HFS-B 6 trials 6 trials HFS Cond 6 trials T 2 trials C T3 2 trials T6 2 trials Fig.. Treatment protocols. A: supraorbital branch of the trigeminal nerve (SO)-evoked reflex blink with R and R2 components. B: high-frequency stimulation (HFS) presented before R2 (HFS-B). C: HFS presented after R2 (). D, right: HFS Cond trial, in which HFS-B coterminated with a 3-ms tone. Left: expansion of the same record to show the HFS-B stimulus. E: experimental paradigm. Triangles show a twice-threshold - s supraorbital nerve stimulus. T, T3, T6, immediately, 3 min, and 6 min after treatment. D HFS Cond 25 ms tone 3 ms tone HFS J Neurophysiol doi:.52/jn Downloaded from by ${individualuser.givennames} ${individualuser.surname} ( ) on February 9, 28. Copyright 24 American Physiological Society. All rights reserved.

3 89 MODIFYING REFLEX BLINK GAIN trial consisted of a single SO stimulus at 2T to evoke a reflex blink followed by five 2T SO stimuli delivered at 4 Hz before the onset of the R2 component of the OOemg activity (Fig. B). The latency between first SO stimulus and HFS was adjusted for each rat based on their average R2 latency. The second group (n 8) underwent delay eyelid conditioning (HFS Cond) in which the HFS-B stimulus served as the unconditioned stimulus (US) and a tone served as the conditioned stimulus (CS) (Fig. D). Three rats in the HFS Cond group also contributed to the HFS-B group data. Each of the 6 paired trials consisted of a 3-ms CS with a 4-ms rise time that coterminated with the HFS-B US. Four rats were trained with a 2-kHz tone and the other four with an 8-kHz tone. Both tones were 85 db. Two rats in each CS group developed robust conditioning (see Fig. 4A). During the HFS Cond treatment each day, rats received paired CS-US trials followed by a CS alone on the eleventh trial and a single 2T SO stimulus on the twelfth trial. We presented 6 of these 2 trial blocks each day, delivering a total of 6 CS HFS-B, 6 CS alone, and 6 SO alone trials. Thus rats in the HFS-Cond treatment received the same number of HFS-B stimuli as rats in the HFS-B alone condition. In the third group (n 4), HFS occurred after the R2 component of the blink reflex (). This protocol did not modify blink amplitude in humans (Mao and Evinger 2) and served as a control for the number of stimuli delivered in the other treatment conditions. Rats received 6 trials of stimulation in which the HFS occurred after termination of the R2 blink component (Fig. C). Latency between first SO blink evoking stimulus and HFS was adjusted for each rat based on the average latency and duration of the R2 component. Data collection and analysis. Reflex and conditioned blinks were monitored as the rats moved freely in their home cage in a darkened room during their subjective night. OOemg signals were amplified (A-M Systems model 7; four-channel differential amplifier), filtered at.3 5 khz, collected at 4 khz per channel (DT 283, Data Translation, Marlboro, MA; 2-bit analog-to-digital resolution), and stored for later off-line analysis on laboratory-developed software. Blink amplitude was determined by integrating the rectified OOemg activity between the beginning and end of each blink component (Dauvergne and Evinger 27; Evinger et al. 99; Pellegrini et al. 995; Pellegrini and Evinger 995). Because stimulus intensity was constant within a day, changes in blink amplitude after HFS were changes in trigeminal blink reflex gain. We normalized within-day treatment-induced changes in blink amplitude by dividing both the average pre-hfs and the average post-hfs EMG amplitude by the median pre-hfs EMG amplitude for that day. As there were no consistent differences in data between post-hfs blocks, we combined them for analysis. We quantified the effect of the HFS treatment by subtracting the normalized pre-hfs blink amplitude from the normalized post-hfs blink amplitude and termed this measure relative gain change. A negative value demonstrated a decrease in blink gain, whereas a positive value indicated an increased blink gain. We calculated two measures of long-term modifications induced by HFS treatment. For each day of treatment, we determined the mean amplitude of 2T SO-evoked blinks before treatment relative to the median pretreatment amplitude on the first day of testing. If treatment produced a long-term depression in the trigeminal blink amplitude, then pretreatment blink amplitude should decrease across days of HFS treatment. Second, to determine whether the HFS treatment created a larger relative change after repeated days of treatment, we plotted the relative change each day as a function of the number of days of treatment. If the treatment became more effective at modifying trigeminal reflex blink gain after repeated treatments, then there should be greater relative change across days of treatment. In addition to reflex blinks, conditioned responses (CRs) in the HFS Cond treatment were analyzed. OOemg activity in the first 35 ms of tone onset was designated as an alpha response and not analyzed. A CR was defined as OOemg activity 5 standard deviations above the baseline OOemg activity determined from the ms of OOemg activity prior to tone onset. Trials in which OOemg baseline activity was disrupted by other activities, e.g., face cleaning, were not analyzed. Percent CRs was calculated as the number of trials exhibiting a CR divided by the total number of useable CS trials. Statistical tests of significance (P.5) were performed with SPSS software (SPSS, Chicago, IL) using an independent-samples t-test or a paired-samples t-test. Data are presented as means SE. Regressions were performed with linear, power, logarithmic, and exponential curves. The function generating the highest correlation was employed to describe the data. RESULTS In humans, the HFS-B condition depressed the R2 component of subsequent blinks (Mao and Evinger 2). The R response, however, is not reported in this study because the R response is inconsistent and provides very little lid closure in primates (Evinger et al. 99; Snow and Frith 989). In mammals other than primates, however, the R component provides most of the lid closure (Pellegrini et al. 995), whereas the R2 response is weak and inconsistent (Anor et al. 996, 2; Basso et al. 993; LeDoux et al. 997; Pellegrini et al. 995). Thus we investigated changes in R magnitude. Averaged across all rats and days, HFS-B treatment significantly depressed subsequent condition R and test R responses by % [t (5) 9.7, P.; Fig. 2A, left] and % [t (5) 2.2, P.5; Fig. 2A, left], respectively, relative to pre-hfs-b R values. Before HFS treatment, a condition R2 blink response occurred on slightly more than half of the trials, 57 3%. After HFS-B treatment, the probability of a condition R2 occurring fell to 8.4%, significantly less than before treatment [t (5) 5.3, P.]. As with humans, treatment did not alter the R2 component of blinks (Mao and Evinger 2); treatment in the rat also did not significantly affect R blink amplitude (Fig. 2A, right). Averaged across all rats and days of treatment, treatment produced an insignificant % decrease [t (4).53, P.5; Fig. 2A, right] and % increase [t (4).46, P.5; Fig. 2A, right] in condition R and test R blink amplitude, respectively. Thus, as occurred with the human R2, the timing of the HFS relative to the R2 determined the effect of HFS on trigeminal blink circuits. In the rat, HFS-B treatment created a short-term reduction in R blink gain. Averaging across days, however, obscured two long-term modifications in trigeminal blink circuits created by repeated HFS-B treatment. First, R blink amplitude collected before HFS-B treatment decreased with repeated days of HFS-B treatment (Fig. 2B). Normalizing the mean R blink amplitude before HFS-B each day to the median R blink amplitude before HFS-B on the first day of treatment revealed a significant exponential decrease across days of HFS-B treatment for both condition R (r 2.49, P.5; Fig. 2B) and test R (r 2.4, P.5; Fig. 2B) blink amplitude. The second long-term modification caused by repeated HFS-B treatment was the enhanced ability of HFS-B treatment to cause relative gain decreases (r 2.3; Fig. 2C). Over the first 3 days of HFS-B treatment, the rats showed an average relative change in condition R amplitude of 6%, whereas over the last 3 days of HFS-B treatment the average relative change was 27%. The J Neurophysiol doi:.52/jn Downloaded from by ${individualuser.givennames} ${individualuser.surname} ( ) on February 9, 28. Copyright 24 American Physiological Society. All rights reserved.

4 Relative Change Pre HFS re Day Relative Change A * B C HFS-B 3 2 RC RT Days of HFS-B R2 Probability MODIFYING REFLEX BLINK GAIN 4 D HFS-B E F Days of HFS Days of 89 Fig. 2. A: average relative change in condition R (RC) and test R (RT) blink amplitude following HFS treatment. B: average pre-hfs blink amplitude relative to median pre-hfs blink amplitude on day for RC and RT as a function of days of HFS-B treatment. Lines are best fit exponentials. C: average relative change for RC and RT as a function of days of HFS-B treatment. Lines are logarithmic best fits. D: average condition R2 (R2C) probability as a function of days of HFS-B and treatment. Lines are best fit linear regressions. E: average pre-hfs blink amplitude relative to median pre-hfs blink amplitude on day for RC and RT as a function of days of treatment. F: average relative change for RC and RT as a function of days of treatment. *P.5, P.. Error bars are SE Days of HFS-B Days of test R showed a similar, but smaller, change in relative gain across days of treatment (r 2.4; Fig. 2C). Repeated days of HFS-B decreased the probability of an R2 occurring before HFS-B treatment (r 2.73, P.; Fig. 2D). Thus the decreasing R blink amplitude and R2 probability demonstrated that repeated HFS-B treatment reduced trigeminal drive on blink circuits. In addition, repeated HFS-B treatment made daily HFS-B treatment more effective at decreasing blink gain. Consistent with the absence of short-term effects (Fig. 2A, right), repeated days of treatment failed to produce long-term changes. Repeated days of treatment did not change pretreatment amplitude relative to day pretreatment blink amplitude for condition R (r 2.3, P.5; Fig. 2E) or test R (r 2.2, P.5; Fig. 2E). Likewise, R2 probability did not change with repeated days of treatment (r 2.7, P.5; Fig. 2D). There were also no consistent modifications of relative change across days for condition R (r 2.3, P.5; Fig. 2F) or test R (r 2.27, P.5; Fig. 2F). Indeed, the variability of the data demonstrated that when the HFS occurred after the R2 component it produced no consistent effect on blink amplitude (Fig. 2, E and F). Thus the absence of long-term modifications with treatment demonstrated that the long-term reductions in blink gain created by HFS-B treatment were the result of the timing of HFS relative to the R2 and not generalized habituation. Repeated HFS-B or treatment did not modify blink threshold (Fig. 3A). For each rat and condition, every day s reflex blink threshold was normalized to the threshold current on the first day of treatment. These relative thresholds were averaged across rats for each day of treatment. Blink threshold did not change significantly with repeated days of HFS-B treatment (r 2.32, P.5; Fig. 3A), indicating that the long-term changes in trigeminal blink amplitude were not the result of a change in blink threshold. Likewise, repeated treatment did not alter blink threshold (r 2.3, P.5; Fig. 3A). Thus the HFS-B paradigm produced both shortand long-term decrease in the gain of trigeminal reflex blinks without affecting blink threshold. Delay eyelid conditioning (HFS Cond). The cerebellum plays a critical role in eyelid conditioning (Christian and Thompson 23; De Zeeuw and Yeo 25; Raymond et al. 996; Thompson 25) and trigeminal reflex blink adaptation (Chen and Evinger 26; Evinger and Manning 988; Pellegrini and Evinger 997). If short- and long-term HFS-B depression of trigeminal reflex blinks involves some of the same cerebellar neurons responsible for delay eyelid conditioning, then some occlusion should occur when HFS-B treatment and delay eyelid conditioning are combined, because HFS-B depresses blink circuits (Fig. 2A, left) whereas eyelid conditioning increases the activity in blink circuits. If occlusion occurred between the two paradigms, then we expected that rats failing to develop eyelid conditioning would generate small gain decreases whereas rats that developed robust eyelid conditioning would not show blink gain changes with HFS Cond treatment. Using 7% CRs as a threshold for the devel- J Neurophysiol doi:.52/jn Downloaded from by ${individualuser.givennames} ${individualuser.surname} ( ) on February 9, 28. Copyright 24 American Physiological Society. All rights reserved.

5 892 MODIFYING REFLEX BLINK GAIN Relative Blink Threshold A B HFS-B <7% HFS Cond >7% HFS Cond Days of HFS Fig. 3. A: mean blink threshold relative to day blink threshold as a function of days of HFS-B and treatment. B: mean blink threshold relative to day blink threshold as a function of days of HFS Cond treatment for rats achieving 7% conditioned responses (CRs) and rats achieving 7% CRs. Error bars are SE. opment of robust eyelid conditioning, we found four rats that consistently achieved 7% CRs (Fig. 4A) and four rats that failed to attain this level of conditioning ( 7% CRs; Fig. 4A). Averaged across all days and rats that exhibited 7% CRs, condition R amplitude decreased by.8 5.3% after HFS Cond [t (49) 2.25, P.5; Fig. 4B]. As predicted, this decrease in condition R amplitude was significantly smaller than the depression produced by HFS-B treatment alone [t (43) 3., P.]. For rats that achieved 7% CRs, however, neither condition (RC) nor test (RT) R amplitude changed significantly [t (5).2, P.5, RC; t (5).2, P.5, RT; Fig. 4B, 7%]. Comparing blink depression caused by HFS-B treatment with that caused by HFS Cond treatment in the three rats that participated in both experiments reinforced the differences between the two treatments on blink gain. The blink depression with HFS-B treatment for these three rats was not different from that for rats that received HFS-B treatment alone [RC, t (4).6, P.5; RT, t (4).5, P.5; Fig. 4B, HFS-B*]. In the HFS Cond treatment, these rats exhibited less blink depression than they developed in the HFS-B treatment and were similar to the 7% group (Fig. 4B, HFS-Cond*). Thus combining the HFS-B stimulus with eyelid conditioning disrupted both forms of motor learning. The long-term changes with repeated HFS Cond treatment were smaller than those with HFS-B treatment. Looked at across days of HFS Cond treatment, neither the 7% nor the 7% group exhibited a significant change in pre-hfs condition R amplitude (Fig. 4C; 7% r 2.4, P.5; 7% r 2.4, P.5). Blink threshold also did not change across days of HFS Cond (Fig. 3B; r 2.28, P.5, 7%; r 2.22, P.5, 7%). There was a trend for relative change to become more negative across days of HFS Cond treatment for the 7% group (Fig. 4D; r 2.8, P.5) but to become more positive for the 7% group (Fig. 4D; r 2.2, P.5). The trend for larger relative changes across days of treatment for the 7% group was confirmed by averaging relative change across days (Fig. 4E). Averaged over the first 4 days of HFS Cond treatment there was no short-term relative change for rats in the 7% group [.3., t (5).26, P.5; Fig. 4E, 7%], but averaged over days 8 2 the relative change was.23.8 [t (5) 3., P.; Fig. 4E, 7%]. Thus it took at least 8 days of HFS Cond treatment before these rats exhibited a significant short-term decrease in condition R blink gain. In contrast, HFS-B treatment produced a relative change in condition R blink gain of.2.4 [t (43) 4.9, P.; Fig. 4E, HFS-B] in the first 4 days of treatment, and by days 8 2 the mean relative change was.3.5 [t (23) 6., P.] over all of the rats receiving HFS-B. In rats that achieved 7% CRs and rats receiving treatment, there were no significant changes in condition R gain in either the first 4 or the last 4 days of treatment (Fig. 4E). Consistent with occlusion occurring between HFS-B and eyelid conditioning, rats that failed to acquire robust eyelid conditioning only slowly developed short-term gain reductions and never exhibited long-term changes and rats that acquired robust eyelid conditioning never exhibited shortor long-term reductions in blink gain. DISCUSSION HFS treatment in which the 4-Hz SO stimulus train occurs before the R2 component of a SO-evoked blink produces both short- and long-term decreases in trigeminal reflex blink amplitude (Fig. 2, Fig. 4E) independent of short- or long-term habituation (Abel et al. 998; Ferguson et al. 978; Hirano et al. 996; Sanes and Ison 983). For example, matched for the number of HFS-B trains does not cause short (Fig. 2A)- or long (Fig. 2, E and F)-term decreases in blink amplitude. Further evidence against habituation causing the blink depression demonstrated here is that potentiation rather than habituation develops when the HFS occurs during the R2 blink component in humans (Battaglia et al. 26; Crupi et al. 28; Kranz et al. 23; Mao and Evinger 2; Quartarone et al. 26). Independent of the number of HFS trains, however, habituation can occur when the intertrial interval used to collect pre- and post-hfs data is s (Zeuner et al. 2) instead of the 2-s intertrial intervals used in this and other HFS studies. Thus the timing of the HFS relative to the R2 is critical in creating the short- and long-term decreases in blink reflex amplitude. This reduction is a modification of reflex blink gain, because blink amplitude decreases while the blinkevoking stimulus remains constant (Fig. 3A). HFS treatment in humans (Battaglia et al. 26; Crupi et al. 28; Mao and Evinger 2; Quartarone et al. 26; Suppa et al. 2; Zeuner et al. 2) focuses exclusively on changes in the R2 component of SO-evoked blinks (Evinger et al. 99; Snow and Frith 989). In this study, we primarily measured changes in the R component because it produces most of the lid closure in nonprimate mammals (Pellegrini et al. 995). Our data demonstrated that the R showed the same pattern of gain changes as the R2 in human HFS paradigms. The demonstration that R and R2 responses of SO-evoked blinks arise from J Neurophysiol doi:.52/jn Downloaded from by ${individualuser.givennames} ${individualuser.surname} ( ) on February 9, 28. Copyright 24 American Physiological Society. All rights reserved.

6 MODIFYING REFLEX BLINK GAIN 893 Percent CRs Pre HFS re Day Relative Change 75 5 A 25 <7% >7% C.5 <7% >7% E Days of HFS Conditioning Days - 4 Days Days of HFS Conditioning -A ** HFS-B <7% >7% Relative Change Relative Change B.2 HFS-B HFS-B* HFS-Cond* <7% >7% D * Days of HFS Conditioning * RC RT * <7% >7% Fig. 4. A: % CRs as a function of days of HFS Cond for rats that achieved 7% CRs and rats that achieved 7% CRs over 2 days of HFS Cond treatment. B: average relative change in RC and RT blink amplitude following HFS treatment for all HFS-B rats, HFS-B treatment for the 3 rats that participated in both HFS-B and HFS Cond treatments (HFS-B*), HFS Cond treatment for the 3 rats that participated in both HFS-B and HFS Cond treatments (HFS-Cond*), and for all 7% and all 7% rats. C: mean pre- HFS blink amplitude relative to median day pre-hfs blink amplitude as a function of days of HFS Cond treatment for rats that achieved 7% CRs and rats achieving 7% CRs. D: mean relative change as a function of days of HFS Cond treatment for rats in the 7% and 7% groups. Lines are best fit linear regressions. E: mean relative change over days 4 and days 8 2 for all rats in the HFS-B,, 7%, and 7% groups. Error bars are SE. *P.5, **P., P.. different regions of the trigeminal complex and involve different pools of medullary interneurons (Cruccu et al. 25; Ongerboer de Visser 983; Ongerboer de Visser and Kuypers 978; Pellegrini et al. 995) suggests that the blink changes created by HFS treatment reflect modifications in second-order trigeminal neurons and/or OO motoneurons. Repeated HFS-B treatment produced a cumulative depression of trigeminal reflex blink circuits. Over days of HFS-B treatment, trigeminal blink drive fell exponentially, so that a 2T SO stimulus evoked smaller blinks (Fig. 2B). HFS-B treatment also became increasingly effective at reducing blink gain over days of HFS-B treatment (Fig. 2C). Just as facial palsy creates a long-term enhancement of blink circuits (Cossu et al. 999; Manca et al. 2; VanderWerf et al. 27), repeated HFS-B treatment produced a longterm depression of blink circuits. Thus repeated HFS-B treatment created long-term modifications of trigeminal blink circuits and blink gain equivalent to those caused by facial palsy. The cerebellum plays a major role in gain modification of trigeminal reflex blinks, saccadic eye movements, and the vestibuloocular reflex (Blazquez et al. 24; Boyden et al. 24; Lisberger 988; Miles and Lisberger 98; Pellegrini and Evinger 997; Rambold et al. 22; Raymond et al. 996; Robinson et al. 22; Scudder and McGee 23; Soetedjo and Fuchs 26; Straube et al. 2). The present data suggest that the cerebellum also participates in gain modification created by HFS-B treatment. The activity of interpositus neurons changes with trigeminal blink gain modification (Chen and Evinger 26) and delay eyelid conditioning (Freeman and Nicholson 2; Gould and Steinmetz 996; Kim and Thompson 997; Nicholson and Freeman 24). If HFS-B gain modification also involves some of the same pools of interpositus neurons as delay eyelid conditioning, then competition between HFS-B treatment to depress blink circuits and delay eyelid conditioning to excite blink circuits should affect both types of learning. In one group of rats, 7%, robust conditioning did not occur, as evidenced by the inability to exhibit at least 7% CRs in 2 days of conditioning. Thus the excitatory drive created by delay conditioning competed only partially with the decreased drive of HFS-B on blink gain. This competition slowed the development of short-term changes in blink gain and prevented long-term changes (Fig. 4). Rats developing 7% CRs exhibited rates of eyelid conditioning similar to those reported in other studies of eyelid conditioning (Freeman et al. 27; Green et al. 2; Lee and Kim 24; Weiss and Thompson 99). In these rats, eyelid conditioning won the competition for neural resources, blocking short- and long-term blink depression normally produced by HFS-B treatment. The long-term changes created by repeated days of HFS-B treatment suggest the possibility of reducing trigeminal activity in the focal dystonia benign essential blepharospasm (BEB), involving trigeminal overactivity (Hallett et al. 28) and cerebellar abnormalities (Kerrison et al. 23; Obermann et al. 27). The visually disabling spasms of lid closure that characterize BEB appear to be an exaggeration of blink adaptations to dry eye or eye irritation (Evinger et al. 22; Hallett et al. 28) initiated in trigeminal blink circuits (Henriquez and Evinger 27; Schicatano et al. 22) and probably sustained J Neurophysiol doi:.52/jn Downloaded from by ${individualuser.givennames} ${individualuser.surname} ( ) on February 9, 28. Copyright 24 American Physiological Society. All rights reserved.

7 894 MODIFYING REFLEX BLINK GAIN by modifications in cerebellar activity (Evinger et al. 989; Pellegrini and Evinger 997). Other characteristics of BEB, photophobia and excessive blinking, also reflect exaggerated excitability of trigeminal blink circuits (Dolgonos et al. 2; Kaminer et al. 2). A single day of HFS-B treatment, however, does not produce significant clinical changes in lid symptoms, although patients report feeling considerably better after HFS-B (Kranz et al. 23). The lack of a significant reduction in lid abnormalities with a single treatment may reflect the inability of one HFS-B treatment to modify cerebellar circuits significantly. Repeated HFS-B treatment, however, clearly modifies cerebellar circuits to create an exponential reduction in the activity of trigeminal blink circuits and with repetition becomes increasingly effective at short-term gain decreases. This long-term change may be effective at reducing spasms of lid closure and other trigeminal abnormalities. Because BEB patients exhibit enhanced learning in HFS paradigms (Quartarone et al. 26), repeated HFS-B treatment may produce more long-term blink circuit depression than occurs in normal subjects. ACKNOWLEDGMENTS We thank Donna Schmidt and Pratibha Thakur for technical assistance and Dr. Alice S. Powers, Pratibha Thakur, and Mala Ananth for helpful comments on earlier versions of this manuscript. Present address of M. Ryan: Cold Spring Harbor Laboratory, Bungtown Rd., Cold Spring Harbor, NY 724. GRANTS This work was supported by grants from the National Eye Institute (EY- 739) and the Thomas Hartman Center for Parkinson Research to C. Evinger and National Institute of Neurological Disorders and Stroke Grant F3 NS to J. Kaminer. DISCLOSURES No conflicts of interest, financial or otherwise, are declared by the author(s). 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