49 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /0, No. +,, 0// 0/3 (,**3)

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1 49 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /0, No. +,, 0// 0/3 (,**3) 655 B,B,C,E +, DPPH ORAC * + *, ++, -Diphenyl-,-picrylhydrazyl Radical-scavenging Capacity and Oxygen Radical Absorbance Capacity of Sweet Potato Cultivars with Various Flesh Colors Tomoyuki Oki, Maki Sato, Masaru Yoshinaga, -. 0 Tetsufumi Sakai, Terumi Sugawara, Norihiko Terahara* and Ikuo Suda National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Food Electron Transfer, ET Research Organization,,.,+ Suya, Hydrogen Atom Transfer, HAT Koshi, Kumamoto , 1 * Department of Food Science for Health, Faculty of Health and Nutrition, Minami-Kyushu University, / +, Kirishima, Miyazaki 22* **-,, ++, -Diphenyl-,-picrylhydrazyl (DPPH) radical-scavenging. to capacity and oxygen radical absorbance capacity (ORAC). / of ten sweet potato cultivars with various flesh colors DPPH ABTS were measured. The DPPH radical-scavenging activity 0 (DPPH-RSA) was almost the same among the sweet potato cultivars with white, yellow, and orange flesh ; however, purple-fleshed sweet potato cultivars showed about +* times higher activity than those with other flesh colors. A ET ++, -diphenyl-,- similar tendency was observed for ORAC. Therefore, picrylhydrazyl DPPH HAT electron and hydrogen atom transfer reaction-based assays could both detect the high antioxidant activity of Oxygen Radical Absorbance Ca- purple-fleshed sweet potatoes. Moreover, a high correlation pacity, ORAC between the DPPH-RSA and ORAC values was found for three cultivars and seventeen breeding lines of purple- fleshed sweet potato (R *. 23. ). Based on these results, DPPH ORAC our DPPH radical assay appears e# ective as an alternative method for estimating ORAC values of purple-fleshed + sweet potatoes. (Received Jul. +1,,**3 ; Accepted Aug. +1,,**3),**1 +* Keywords : sweet potato, antioxidant activity, ++, -diphenyl-,-,1 picrylhydrazyl radical, oxygen radical absorbance capacity, anthocyanin : DPPH ORAC +. Ipomoea batatas L ,,.,+ * 22* **-, / +, Corresponding author tomooki@a# rc.go.jp, cm

2 656 /0 +,,**3 +, 50 +* Table + DPPH radical-scavenging activities and ORAC,* values of sweet potato cultivars and breeding lines with various flesh colors DPPH radical- ORAC value Cultivar flesh scavenging activity ( mol-te DPPH,,, -azobis (,-amidino-propane) dihydrochlo- Breeding line color ( mol-te/ +** g-fw) / +** g-fw) ride AAPH 0 -hydroxy-,/12,,, - Ayamurasaki purple +32,,/3 1+0, +-3, tetramethylchroman-, -carboxylic acid Trolox Murasakimasari purple,/*, -0, 33,/ +/0* Fluorescein Na salt Aldrich, - Akemurasaki purple,,03,.1 3*, morpholinoethanesulfonic acid MES Hamakomachi orange,/ Ayakomachi orange +3* +. /,2 3/ Beniharuka yellow,*- -. /3. 33 Koukei +. yellow,*2,2 / , M cm Koganesengan white +1* +-./1 22 Joy white white,.1,0 /20.1 Daichinoyume white +2/ Wu Kyukei *+,,0-+ purple /,2/ 0+- +/,2/ +1/2 Kyukei *,,,2-. purple.3*+ / , -.01 Dionex ASE-,*g A,, -+* purple./*+ 0,0 +,120-3// Kyukei *,,-.-. purple.2* *-, : + : + Kyukei *,,---, purple.-, : : 1* :,3./ : *./ Kyukei *,,,2-, purple.,.* -+1 +/,02 +.*, AWA Kyukei *,,,2-3 purple.*0-,,* +/ Kyukei 32+0* -+ purple -22, * Kyukei *,,,2--, purple -0/, 1*3 +.,.1,,11 : : 2/ : +../ : Kyukei *+,---+, purple -/* / */. Wu Kyukei *,,,2-,1 purple --.+,3. +,//+ +/2. DPPH A-. -- purple -,,/ DPPH A-. -. purple,/2.,3, 3,+/ 3,. +* Kyukei *,,+*-1 purple,/2,,, *.* DPPH A, -- purple,-*+,-* 0-/, 3,, +** g-fw Trolox Kyukei *,+33-- purple +2*3 +* *33 A+3 -+ purple +1+/,-. //,* +*+1 ORAC Mean valuessd of four to seven tubers4 ++ +, ORAC ORAC +** g-fw Trolox DPPH ORAC Table + 2 DPPH +- 1 :,*3 mol-te+** g-fw YGM-0 Peonidin --( 0, 0 -ca# eoyl- +* - :,,/+ mol-te/ feruloylsophoroside)-/ -glucoside g-fw ORAC DPPH +** g- FW, Furuta. HAT DPPH AAPH +. +/ USDA ORAC +0 ORAC ET HAT ET HAT, USDA +* ORAC 1/0

3 51 : 657 Table, Anthocyanin content and composition of sweet potato cultivars and breeding lines with purple flesh Cultivar Anthocyanin content (mg/ +** g-fw) Cy Pn Breeding line YGM-+ a YGM-+ b YGM-, YGM-- YGM-. b YGM-/ a YGM-/ b YGM-0 Total ( ) ( ) Ayamurasaki /43 *41 +*42 +4*.242 /40,*4. *41.-4+,4.,*43.4/ * -0+42,+4-,. 10 Murasakimasari 143 *4/ /4* *42.,4/ /4. +.4* +4/ -/4*,4+ /* , / Akemurasaki ,4* 3*42 34.,,40 +4.,,4*.4,.+4/ -42 +/-40 +/43./43,4,.*+4* Kyukei *+,,0-+ ++*4+,* ,+42,24* +/-41 24/ , ++4* 2*4/ +04* *4. 3*/42 +*,40 1/,/ Kyukei *,,, ,*4* +34+,.4*.43 +1*4-., *.+* ,/ , Kyukei *,,-.-. / *4,,1-4/,* ,+41-4, * / /4, /4. 0/*4* +/4, 13,+ A,, -+*,*14*,,4* /* **4/ +*4, +*,4* +,42 / , /43-4* * /+142 /-4/ Kyukei *,,---,,41 *4,.4/ *40 /+41-4-,*43,4/ ,,24,, * --41,,*4, 24* Kyukei *,,,2-,,/4.,40,+4* +4/ / /4. +, ,0*4* +24+,,14* , Kyukei *,,,2-3 +,4., * +* ,40 /14+.4, / -/43 3, ,24/,, 12 Kyukei 32+0* ,4+.* * 14- +* / * , / +,4, /.4+ -* 1* Kyukei *,,,2--, 241,4+ +*42-43 /+40 /42, * -*4* / ,0/ /34/ ++4* 0/141 2/ Kyukei *+,---+, / +242 / / , 24* +0/43,240,*/4- +04/ +3+4*,,4* 2-24/ +*-41,/ 1/ Kyukei *,,,2-,1 ++4/,42 +,4- /41,24/ /4* -/ ,*4/,24, +*/4/,/ ,340 -+, A / 14+ 0*4*,4. +*04/ * ,4+ 2* / /1 A /41,4+, , * +*42 / *4* ,4* 041 /,*40 /+43,0 1. Kyukei *,,+*-1 +.4,,4,., * *-4* +.42 /14/ 242,+4,.4-004, 240 +, / // A-,- +24/.4, /040, ,.*4, / , +4/ +,4*.41 34/.4- -/14/ * 3 Kyukei *, *,4+ *41,,4*,4, * -/ * /*40,*41 +,*4/ +.4* -2/4*.240 +* 3* A ,43 +04, *42 /,40 14+,.42-4*,*4. +41,.4,.43.,43.4., ,,04-,*4+.3 /+ Mean valuessd of four to seven tubers Abbreviations : YGM-+ a, Cyanidin --( 00, -ca# eoyl-p-hydroxylbenzoylsophoroside)-/ -glucoside ; YGM-+ b, Cyanidin --( 00, - dica# eoylsophoroside)-/ -glucoside ; YGM-,, Cyanidin --( 0 -ca# eoylsophoroside)-/ -glucoside ; YGM- -, Cyanidin --( 0, 0- ca# eoylferuloylsophoroside)-/ -glucoside ; YGM-. b, Peonidin --( 0, 0 -dica# eoylsophoroside)-/ -glucoside ; YGM-/ a, Peonidin --( 0, 0 -ca# eoyl-p-hydroxylbenzoylsophoroside)-/ -glucoside ; YGM-/ b, Peonidin --( 0 -ca# eoylsophoroside)-/ -glucoside ; YGM- 0, Peonidin --( 0, 0 -ca# eoylferuloylsophoroside)-/ -glucoside ; Cy, Cyanidin-based anthocyanin ; Pn, Peonidin-based anthocyanin4 + *,* mol-te+** g-fw : 2/2 mol-te+** g-. YGM -. b -/ a -/ b FW -0-0,. YGM-a + -b + -, -- / +./12-3 mol-te +** g-fw - +1 ORAC ORAC / /,* +3 20, mol-te +** g-fw ++ DPPH Table + - YGM-+ YGM-0 Table 2 1*. mol-teg-fw,,,0.- +,3-. 2 mg+** g-fw +* YGM / Wu ORAC.. :. / ORAC ORAC - +1 DPPH +/ 2,* +. /** +* *0* 0 /,* 0,.+ mol-te+** g-fw +0 ORAC,* +*, 2 ORAC

4 658 /0 +,,**3 +, 52 R *. 2-- ORAC ORAC 2 R *. 2-- ORAC Table, DPPH ORAC ORAC R *23.. DPPH 2 ORAC ORAC +1 - DPPH ORAC R * ,+ DPPH ORAC + FFI , Suda, I., Oki, T., Masuda, M., Kobayashi, M., Nishiba, Y. and Furuta, S., JARQ, -1, (,**-). Table, - Kaur, C. and Kapoor, H.C., Antioxidants in fruits and vegetables the millennium s health. Int. J. Food Sci. Technol., -0, 1*-1,/ (,**+). DPPH. Furuta, S., Suda, I., Nishiba, Y. and Yamakawa O., High tert-butylperoxyl radical scavenging activities of sweet +2 ORAC DPPH ORAC / potato cultivars with purple flesh, Food Sci. Technol. Int. Tokyo,., ---/ ( +332). Oki, T., Masuda, M., Furuta, S., Nishiba, Y., Terahara, N. and Suda, I., Involvement of anthocyanins and other DPPH phenolic compounds in radical-scavenging activity of purple-fleshed sweet potato cultivars, J. Food Sci., 01, ORAC +1/, +1/0 (,**,). ORAC 0 Oki, T., Nagai, S., Yoshinaga, M., Nishiba, Y. and Suda, I., Contribution of b-carotene to radical scavenging capacity varies among orange-fleshed sweet potato cultivars, Food Sci. Techonol. Res., +,, +/0 +0* (,**0). 1 3* 1 Prior, R.L., Wu, X. and Schaich, K., Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements, J. Agric. Food DPPH Chem., /-,.,3*.-*, (,**/). 2 Wu, X., Gu, L., Holden, J., Haytowitz, D.B., Gebhardt, S.E., Beecher, G. and Prior, R.L., Development of a database ORAC for total antioxidant capacity in foods : a preliminary study, J. Food Compos. Anal., +1,.*1.,, (,**.). DPPH 3 Wu, X., Beecher, G.R., Holden, J.M., David, B., ORAC Haytowitz, D.B., Gebhardt, S.E. and Prior, R.L., Concentrations of anthocyanins in common foods in the United States and estimation of normal consumption, J. Agric. - Food Chem., /.,.*03.*1/ (,**0). ++ : - :, :, +* DPPH : - DPPH ORAC pp ,**2 DPPH ++ Prior, R.L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B. and Jacob, R.A., Assays for hydrophilic and lipophilic antioxidant +* ORAC capacity (oxygen radical absorbance capacity DPPH ET (ORAC FL) of plasma and other biological and food HAT, samples. J. Agric. Food Chem., /+, -,1--,13 (,**-). +,,*

5 53 : 659 ORAC.1+,**/ +0 http : / / www. ars. usda. gov / SP, UserFiles / Place / pp. 1320,**2 +,-/./** /Data/ORAC/ORAC*1.pdf Wu, X., Beecher, G.R., Holden, J.M., Haytowitz, D.B., Gebhardt, S.E. and Prior, R.L., Lipophilic and hydro- /. ---2,**1 philic antioxidant capacities of common foods in the +. United States, J. Agric. Food Chem., /,,.*,0.*-1 (,**.) /, 00+,**,.1,-1,/,,**3 +/,+ 1 +1, /,.0,