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3 Abstracts NEU-13 Estimation of Pharmacologically Interesting Dose Range and Treatment Regimen of Ascorbic Acid in Mice Naveen Shivavedi 1, Shyam Sunder Chatterjee 2, Vikas Kumar 1 1 Neuropharmacology Research Laboratory, Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi , India, 2 Stettiner Str. 1, Karlsruhe, Germany (Retired Head of Pharmacology Research Laboratories, Dr. Willmar Schwabe GmbH and Co. KG, Karlsruhe, Germany) Objective: L-Ascorbic acid (AA) is the most abundant watersoluble naturally occurring organic compound with antioxidant properties. It serves as a cofactor for enzymes involved in hormone biosynthesis, and the regeneration of antioxidants. Plants and food rich in AA have been widely used in Ayurveda to treat a variety of common and stress related disorders. Materials and Methods: The experimental protocol was designed to evaluate the oral efficacies of AA for putative adaptogenic activity in male mice. A battery of behaviour test models viz. stress-induced hyperthermia, tail suspension test and pentobarbital-induced hypnosis were used to evaluate the adaptogenic activity of AA. Mice were divided in different groups, and were administered with AA (5, 25, 125 and 625 mg/kg) daily as solution (10 ml/kg) prepared with ph balanced distilled water for 11 consecutive days. Body weight and basal rectal temperature was measured to access the changes occurs due to daily foot shock induced stress. Results: Stress-induced hyperthermia was significant (p<0.05) reduced by AA in dose dependant manner and also compensated the body weight and basal rectal temperature due to daily handling and intermittent foot shock induced stress. Daily administration with test drug was also reduced the immobility period in tail suspension test and showed considerable potentiating effect on onset and duration of sleep in pentobarbital-induce hypnosis test in dose dependent manner. Conclusion: These observations suggest that AA has potential for adaptogenic activity, and it could be used against various spectrums of neuropsycopharmalogical associated with environmental stress. NEU-14 Paroxetine Attenuates Modified Stress-Re-Stress- Induced Post-traumatic Stress Disorder-Like Behavioral Symptoms Debapriya Garabadu, Neha Singh, Sairam Krishnamurthy* Neurotherapeutics Laboratory, Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi, Uttar Pradesh, India Objective: To attenuate post-traumatic stress disorder-like behavioral symptoms. Materials and Methods: Halothane, paroxetine. The establishment of an appropriate animal model of post-traumatic stress disorder (PTSD) is necessary to promote better understanding of the mechanisms of the disorder. Although no single widely accepted animal model of PTSD has been established to date, the stress re-stress (SRS) animal model has been partially validated as a model for PTSD. However, there is no chronic model for PTSD-like symptoms. Hence, a modification has been proposed to develop long-term PTSD-like behavioral symptoms in the present study. On day-1 (D-1) rats underwent for training session in elevated-plus maze (EPM) test. On D-2, rats were subjected to stress protocol of 2 h restraint and 20 min forcedswim followed by halothane anesthesia. The rats were exposed to re-stress (forced-swim) on D-8 and at six day intervals on D-14, D-20, D-26 and D-32. The rats were treated with paroxetine (PAX; 10.0 mg/kg; p.o.) from D-8 to D-32. Results: Paroxetine attenuated depressive-like symptom in terms of increase in immobility period in forced-swim test in SRS exposed rats from D-14 to D-32. Paroxetine exhibited anxiolytic activity in terms of decrease in the percentage entries and time spent into open arm in EPM in modified SRS exposed animals. Moreover, paroxetine also improved the cognitive deficits in terms of increase in the percentage decrease in the novel arm entries into novel arm in Y-maze in modified SRS rats. Conclusion: These observations emphasize the fact that paroxetine could be an alternative candidate in the long-lasting PTSD. NEU-15 Stress Response Desensitizing Efficacies of Triethylene Glycol and Quercetin in Mice. Nikita Shrivastava 1, Shyam Sunder Chatterjee 2, Vikas Kumar 1 Neuropharmacology Research Laboratory, Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi, India, 2 Stettiner Str. 1, Karlsruhe, Germany (Retired Head of Pharmacology Research Laboratories, Dr. Willmar Schwabe GmbH and Co. KG, Karlsruhe, Germany) Objective: Triethylene glycol and quercetin are two antimicrobial and cyto-static agents encountered also in the adaptogenic herb Withania somnifera. Aim of this study was to compare their efficacies in a mouse bioassay for stress response modulating efficacies of test agents. Materials and Methods: Groups of mice were orally treated with graded daily oral doses of triethylene glycol, or quercetin, or with their vehicles for 11 consecutive days. They were subjected to a foot shock stress triggered hyperthermia test on the 1 st, 5 th, 7 th and 10 th days of treatments, and to tail suspension test on the 11 th day of the treatments. One day after these tests, effects of pretreatments on pentobarbital induced sleep was quantified. Results: Efficacies of both the test agents for inhibiting transient foot shock triggered hyperthermia increased with their increasing daily doses, and their dose dependant antidepressant like effects in tail suspension test were apparent after their 11 daily doses. Daily handling and intermittent foot shock stress triggered looses in body weights and slight elevation of basal core temperatures observed in vehicle treated mice were also dose dependently reversed by both of them. Minimum effective daily oral dose of triethylene glycol for antagonizing pentobarbital hypnosis was 20 mg/kg, whereas that of quercetin was 400 mg/ kg. Conclusion: Both triethylene glycol and quercetin are two stress response modulating secondary metabolites of Withania somnifera, whereupon triethylene glycol is orally more effective. Triethylene glycol is a structurally simpler and functionally novel therapeutic lead from this well known Ayurvedic medicinal plant. NEU-16 Resveratrol Inhibits Behavioral and Neuroinflammatory Changes in Oxaliplatin Induced Sensory Neuropathy Indian Journal of Pharmacology December 2014 Vol 46 Supplement S91
4 ~~COLOGIc. q; ~"~~. ~<~ c::> (') -~ -m Q V ~ ~ ~ 47th Annual Conference of Indian Pharmacological Society (28th -30th December 2014) Gauhati Medical College, Guwahati GMC Guvvahatf NIPER G-uwahati Cszrtifieetsz of Tlpprszeietion Tlweracszd,--to Dr./Mr./Mrs.1Ms." ~A SHR'''ASiflltA'--- _ in acknowledgement of your contribution to the Sclentiftc Program of - IPSCON 2014 B Delegate as DChairperson DJudge B Presenter of a Paper -' in OrM I Poster Session Assam Council of Medical Registration has granted ~ credit hours for this program vide letter No. MCI-Academics/2013/31228? Dr. B. Dinesh Kumar President Indian Pharmacological Society ~~ Dr.K. L. Talukdar Registrar ACMR ~~ k Dr. T. K. Mandai Dr.B. K. Bezbaruah General Secretary_ Organizing Secretary, Indian Pharmacological Society IPSCON -2014,Guwahati
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