Appendix - I. Composition of media

Transcription

1 138 Appendix - I Composition of media 1. Corn meal agar a. Corn meal 40 g b. Dextrose 20 g c. Agar 20 g d. Distilled water 1000 ml 2. Lima bean agar a. Lima bean (Ready-made) 23 g b. Distilled water 1000 ml 3. Modified lima bean agar Amended with tomato 10 per cent 4. Malt extract agar a. Malt extract 20g b. Agar 20g c. Water 1000ml 5. Oat meal agar a. Rolled Oats 70 g b. Dextrose 5 g c. Agar 20 g d. Distilled water 1000 ml 6. Potato dextrose agar a. Peeled potato 200 g b. Dextrose 20 g

2 139 c. Agar 20 g d. Distilled water 1000 ml 7. V 8 juice a. V 8 Juice (100 ml) 8.3 g b. L- asparagine 10.0 g c. Calcium carbonate 2.0 g d. Glucose 2.0 g e. Yeast extract 2.0 g f. Agar 20 g g. Distilled water 1000 ml 8. Nutrient Agar a. Beef extract 3g b. Peptone 5g c. Agar 20g d. Nacl 5% e.water 1000ml

3 140 Appendix - II Composition of organic inputs 1. Agneyastra a. Ipomea leaves 500 g b. Darek leaves (Melia azaderach) 2500 g c. Hot chillies ( Capsicum annuum) 250 g d. Garlic (Allium cepa) 250 g e. Cow urine 5 l f. Water 6 l Boil the whole content to its half and dilute to 1:20 with water before use. 2. Akshwan a. Aak powder( Calotropis sp.) 400 g b. Agnihotra ash 5 g c. Reetha powder 400 g d. Cow urine 1 L e. Water 4 L 3. Amritastra a. Black Pepper 50 g b. Garlic clove 50 g c. Bichhoo buti( Artica dioica) 500 g d. Datura 500 g e. Lemongrass 500 g f. Bana (Vitex negundu) 100 g g.cow dung h.ash i.water 2 kg 5 g 5000 ml

4 Amritpani a. Cow dung 10 kg b. Honey 500 g c. Cow ghee 250 g Make a paste and dilute with 200 l water and sprinkle over 1 acre. 5. Beejamrit a. Cow dung 5 kg b. Cow Urine 5 L c. Cow Milk 1 L d. Lime (deactivated) 250 g e. Water 100 L 6. Fermented butter milk (FBM) a. Fermented butter milk 2 L b. Darek (Melia sp.) 200 g c. Water 4 L Ferment butter milk in an earthen pot for days, dilute 1:20 for foliar spray. 7. Compost - Tea a. Prepared compost kg b. Water 10 L c. Muslin cloth 1/2 m d. Bucket 15 L capacity 8. Darekastra( Melia azaderach) a. Darek leaves 5 kg b. Cow urine 5 L

5 142 c. Cow dung 2 kg Strain after 3 days and dilute with water 10 times. 9. Dashparni (Leaves of following) a. Darek 400 g b. Ipomea 100 g c. Ghaniri(Polygonum sp.) 100 g d. Walnut (Juglans sp.) 100 g e. Bichhoo Buti 100 g f. Lantana 100 g g. Eupatorium 100 g h. Bana 100 g i. Marigold (Tagetes sp.) 100 g j. Garlic ( A. cepa) 250 g k. Hot chilli leaves ( C. annuum) 250 g l. Water 10 L m. Cow urine 2 L Mix all the ingredients thoroughly by crushing the leaves. Ferment the content for 1 month with intermittent stirring. Strain the content and filtrate can be stored up to 6 months. Dilute 1:10 with water. Keep the material in shade. 10. Ghaniri extract (Polygonum sp.) a. Ghaniri leaves 2 kg b. Cow urine 4 L c. Water 4 L

6 Himsol a. Vermi compost 90 kg b. Cow Dung 75 kg c. Cow Urine 15 L d. Ash 500 g e. Shriyantra 1 (one) f. Water 200 L 12. Jeevamrit a. Cow dung 5 kg b. Cow urine 5 L c. Jaggery 1 kg d. Gram flour 1 kg e. Live soil 500 g f. Ash 25 g g. CPP 100 g h. Water 100 L 13. Keetmar a. Safeda leaves( Eucalyptus) 500 g b. Horsetail leaves 500 g c. Lemon Grass 500 g d. Reetha powder 100 g e. Ash 5 g f. Turmeric powder 100 g g. Mint Powder 10 g

7 144 h. Cow dung 1 kg i. Cow urine 5 L j. Water 5 L 14. Lantana extract a. Lantana leaves 3 kg b. Cow urine 12 L c. Water 12 L 15. Matkakhad a. Cow dung 5 kg b. Cow urine 5 L c. Jaggery 250 g d. Water 5 L 16. Neemastra a. Fresh Neem leaves( Azadirachta indica) 5 kg b. Cow Dung 2 kg c. Cow urine 5 L d. Water 2 L Crush 5kg neem leaves in water, add 5l cow urine and 2kg cow dung, ferment for 24 hours with intermittent stirring. Filter the extract and dilute to 100l for foliar spray over one acre. 17. Panchgavya a. Cow dung slurry 4 L b. Cow dung 2 kg c. Milk 2 L d. Curd 2 L

8 145 e. Cow urine 3 L f. Ghee 1 L Mix all the ingredients thoroughly and ferment for 7 days with twice stirring per day. Use as soil amendment with irrigation water (20l/acre)or as seed treatment (soak the seeds for 20 minutes). In modified Panchgavya, oil (Linseed/ Sesame/ Apricot) are used substituting ghee. 18. Sanjeevak a. Cow dung 6 kg b. Cow urine 3 L c. Jaggery 200 g d. Water 10 L 19. Tamra lassi a. Fermented butter milk 10 L b. Darek leaves 200 g Pour the above in copper vessel and bury in soil for 15 days. Dilute 1:20 before use. 20. Vermiwash a. Cow dung (15-20 days old) As per requirement b. Degradable farm waste As per requirement c. Earthworms d. Earthen pot 3 e. Water As per requirement

9 146 III Chemical constituents of healthy cow urine 1. Urine volume ml/kg/day 2. Specific gravity ph Urea nitrogen ml/kg/day 5. Ammonia nitrogen ml/kg/day 6. Total nitrogen ml/kg/day 7. Allnation ml/kg/day 8. Calcium ml/kg/day 9. Chloride mmol/kg/day 10. Coproporphyrin 5-14 microgram/dl 11. Creatine mg/kg/day 12. Magnisium 3.7 mg/kg/day 13. Potassium mmol/kg/day 14. Sodium mmol/kg/day 15. Sulphate 3-5 mg/kg/day 16. Uric acid 1-4 mg/kg/day 17. Uroporphyrin mg/dl 18. Leucocyte <15 micro Lt Soure: samadsetu-gaumata.blogspot.in

10 147 IV ph of fresh cow products 1. Cow milk Butter milk She calf urine Local bull urine Local cow urine Jersey cow urine Jersey bull urine Buffalo urine 8.96

11 148 Brief Biodata of Student Name : Raj Krishan Father s Name : Late Sh. Gian Chand Pruthi Mother s Name : Smt. Kaushalya Devi Date of Birth : Permanent Address : Shri Ram Ashram, Kataula Road, Vill. Chipnu, P.O. Bijani, Distt. Mandi (H.P.) Academic Qualifications: Qualification Year School/Board/Univ. % Division 10 th 1982 A.S. High School, Pundri, Distt. Kaithal (Haryana) 73.2 First B. Sc.(Hons.) Agriculture M. Sc. Plant Pathology Ph. D. Plant Pathology 1987 COA, Kaul, HAU (Hisar) 81.2 First 1991 CCS HAU, Hisar (HR.) 74.6 First 2014 CSK HPKV, Palampur (H.P.) 78.2 First Professional Experience Worked as (i) Field Supervisor, WIMCO Pvt. Ltd. July'1987 to June 1988 (ii) Agriculture Officer in Punjab National Bank from July 1991 to 29th Feb (iii) (iv) Additional Deputy Commissioner, Kullu, before joining Ph.D (HPAS 1996 Batch, ) At present Special Secretary-cum-Additional Director (Environment, Science and Technology) to Govt. of H.P. Fellow/Scholarship/: ICAR Junior Fellowship during M.Sc. Publication: Total: 1 Research Paper: 1