NEW! Micromanipulator meets microscope. Eppendorf Cell Technology

Transcription

1 NEW! Micromanipulator meets microscope Eppendorf Cell Technology

2 Eppendorf Systems... Intracytoplasmic sperm injection (ICSI) Intracytoplasmic sperm injection (ICSI), the injection of a single sperm into an oocyte, has become a powerful means of overcoming male factor infertility. For this application, Eppendorf offers the TransferMan NK 2 micromanipulator. One manipulator is used for moving the holding capillary and a second for transferring the sperm. The oocyte is held in place using the manual injector CellTram Air. Sperm transfer is carried out using the hydraulic manual injector CellTram vario. CellTram CellTram Air, CellTram Oil and CellTram vario manual microinjectors for pressure control, manual microinjection, and liquid dispensing are designed with special emphasis on optimal ergonomics, operational comfort, and high precision. CellTram microinjectors are the ideal complement to your Eppendorf workstation, yet they also fit into all other common micromanipulator workstations. Simple operation and utmost reliability Ultrafine sensitivity through high-precision pressure setting Maintenance-free piston/cylinder system Large, ergonomic dials Universal capillary holder for mounting onto all common micromanipulators CellTram specifications CellTram Air CellTram Oil CellTram vario Method of pressure generation Cylinder/piston system, air-filled, maintenance-free Cylinder/piston system with QuickValve-System, oil-filled, maintenance-free Cylinder/piston system with gear and QuickValve System, oil-filled, maintenance-free Volume alteration per revolution 88 µl 9.5 µl 9.5 µl/950 nl (coarse/fine) Selectable total volume 2,640 µl 950 µl Total piston stroke 15 mm (corresponds to 30 revolutions) 50 mm (corresponds to 100 revolutions) Minimum adjustable volume <200 nl <20 nl < 20 nl/< 2 nl (coarse/fine) Maximum pressure 2,900 hpa 20,000 hpa Additional for IMSI: Objectives Bright field 5x/10x, L 20 40x(Corr) Contrast options IMC (Integrated Modulation Contrast) Other requirements Stage with insert for petri dishes, heated stage or insert Objective Contrast options Other requirements 100x oil DIC MagChange 1.6x, 2.0x, digital camera with C-mount Vario-TV-Adapter 0.33x 1.6x 2

3 ... plus qualified Microscopes... Embryonic stem cell transfer Embryonic stem cell transfer is used to insert, as well as to modify DNA sequences of the animal genome. For the transfer of ES cells into blastocysts, two TransferMan NK 2 devices are used for controlling the holding and transfer capillary. CellTram Air holds the blastocyst while the CellTram vario is used for transferring the ES cells. Eppendorf TransferMan NK 2 This micromanipulator is especially designed for work with suspension cells. It combines simplicity and innovation in a user-friendly system that satisfies highest sophisticated user requirements. With the smooth, stepfree proportional kinetics and menu-driven control, TransferMan NK 2 offers an intuitive operation, allowing the most complex micromanipulation techniques to be carried out rapidly, easily and precisely. One single joystick for coarse/fine movements and control of all axes Proportional kinetics provide intuitive control Automated Home function for fast capillary exchange Individual speed setting allows to work at full capacity Storage of three independent work positions TransferMan specifications Control Via central joystick; proportional kinetics Drive Stepper motor Resolution Approx. 40 nm per micro step Max. movement Approx. 20 mm (each axis) Max. speed of tool 7,500 µm/s Objectives Bright field 5x/10x, L 20 40x Contrast options IMC (Integrated Modulation Contrast), DIC Other requirements Stage with insert for petri dishes, heated stage or insert 3

4 ... result in excellent Workplaces Pronucleus injection The pronuclear microinjection method of producing a transgenic animal is based on the introduction of linear DNA sequences into the chromosomes of fertilised eggs. The TransferMan NK 2 micromanipulator in combination with Leica microscopes, FemtoJet and CellTram Air forms an ideal system to satisfy high demands on accuracy and functionality. FemtoJet This microinjector features a wide range of functionality, simple operation and a built-in compressor to independently deliver the required pressure. It is ideal for injecting aqueous solutions into adherent cells with InjectMan NI 2 and suspension cells (with TransferMan NK 2). The FemtoJet forms the perfect system with Eppendorf micromanipulators, and it integrates just as easily with non-eppendorf microinjection systems. Independent pressure supply with built-in compressor Programmable injection ensures reproducibility Inject and Clean functions triggered via control button on instrument, supplied mouse or optional foot control Electronic linkage to InjectMan NI 2 FemtoJet specifications Pressure supply Integrated compressor Injection time (Ti) s; can be set in increments of 0.1 s Compensation pressure (Pc) Rinsing pressure 0; 5 hpa-max. 6,000 hpa (87 psi); can be set in increments of 1 hpa Max. 7,000 hpa (102 psi) (W H D) cm Weight 6.2 kg Objectives Bright field 5x/10x, L 20 40x Contrast options IMC (Integrated Modulation Contrast), DIC Other requirements Optional fluorescence, temperable stage or insert 4

5 ... meet Microscopes Microinjection into adherent cells Microinjection is one of the core methods to introduce foreign DNA and other non-permeable molecules into cells. Nuclear injection of plasmid DNA enables rapid expression of proteins in specific cells within a population. The menu-controlled, programmable micromanipulator InjectMan NI 2 is ideally suited for microinjection of adherent cells. The electronic connection of the InjectMan NI 2 and the FemtoJet guarantees a very rapid, safe and reproducible microinjection process. InjectMan NI 2 This ultra-precise micromanipulator was designed in response to researchers' suggestions and application requirements. As a result, the InjectMan NI 2 is your ideal partner for microinjection into adherent cells as well as for microinjection into early stage embryos and small organisms. The unique ability to connect the InjectMan NI 2 with our microinjectors FemtoJet and FemtoJet express ensures a very efficient and reproducible processing. Precise coarse/fine control of all axes with one single joystick Axial mounting of the Z-module Can be used with FemtoJet or FemtoJet express for automated axial injection movement Automated Home function for rapid capillary exchange Programmable Z-axis limit for defined injection level avoids capillary breakage InjectMan specifications Control Via central joystick; dynamical kinetics Drive Stepper motor Resolution Approx. 40 nm per micro step Max. movement Approx. 20 mm (each axis) Angle of inclination to object table Max. speed of tool 7,500 µm/s Objectives 10x, L 20 40x Contrast PH (Phase contrast), IPH (Integrated PH) Other requirements Fluorescence, stage with insert for petri dishes 5

6 Micromanipulation competence Ordering information Description Micromanipulator InjectMan NI 2 Your local distributor: Eppendorf AG Hamburg Germany Tel: Fax: eppendorf@eppendorf.com Eppendorf North America, Inc. One Cantiague Road P. O. Box 1019 Westbury, N.Y USA Tel: Toll free phone: Fax: info@eppendorf.com Order no. manipulator for semiautomatic microinjection into adherent cells TransferMan NK 2 micromanipulator for suspension cells with menu-controlled programming adapter A-head 1 for 10 assembly Microscope adapter Leica DM IL LED Leica DMI3000 B/4000 B/6000 B Universal Stand 2, suitable for mounting of manipulators on upright microscopes and stereo microscopes independent of the microscope tripod used Microinjectors FemtoJet, programmable microinjector with integrated pressure supply FemtoJet express, programmable microinjector with external pressure supply CellTram CellTram Air, pneumatic manual microinjector CellTram Oil, hydraulic manual microinjector CellTram vario, hydraulic manual microinjector, with gears Consumables Femtotip, sterile injection capillaries, 0.5 µm inner and 1.0 µm outer diameter, set of Femtotip II, sterile injection capillaries, 0.5 µm inner and 0.7 µm outer diameter, set of Microloader, 2 racks of VacuTip, sterile microcapillaries for holding suspension cells; 35 angle; 15 µm inner diameter, 100 µm outer diameter (set of 25) TransferTip -RP (ICSI), sterile microcapillaries for sperm injection with the ICSI technique; rigid, parallel flange; 0.5 mm long; angle; 4 µm inner diameter, 7 µm outer diameter (set of 25) TransferTip -F (ICSI), sterile microcapillaries for sperm injection with the ICSI flexible flange; 0.4 mm long; 35 angle µm inner diameter, 7 µm outer diameter (set of 25) TransferTip -R (ICSI), sterile microcapillaries for sperm injection with the ICSI rigid flange; 1 mm long; 35 angle; µm inner diameter, 7 µm outer diameter (set of 25) TransferTip (ES), sterile microcapillaries for transferring ES cells into blastocysts; 20 angle; 15 µm inner diameter, µm outer diameter (set of 25) 1 The assembly of the 10 adapters, together with the A-head, enables work with very shallow injection angles (e.g., for injection into the pronucleus of mouse embryos). 2 The universal stand is suitable for the assembly of manipulators independent of the microscope tripod used. For more information, visit our webpages at and eppendorf is a registered trademark. All rights reserved, including graphics and images. Order No. Eppendorf AQ /GB1/3,5T/0809/FEEL/WEID Printed in Germany Order No. Leica AM6000 / Eppendorf, EN Application Support Europe, International: Tel: support@eppendorf.com North America: Tel: ext support_na@eppendorf.com Asia Pacific: Tel: support_asiapacific@eppendorf.com