38 Noble ] mm. Acta Physiologica Sinica Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved. ; icv M ; icv H 1 (250 , NT

Size: px

Start display at page:

Janel Paul
5 years ago
Views:

1 140, , 51 (2), Acta Physiologica Sinica (, ) Sprague2Dawley, (112 g/ kg) (icv) (NT) (10, 20 g), icv 1 (115 g/ 3 l), NT ; icv M (215 g/ 3 l), NT ; icv H 1 (50 g/ 3 l) H 2 (250 g/ 3 l), NT : ; NT, 1 ; NT, M, NT : ; ; ; ; : Q463 (NT) 13,, NT,, [1 4, ], [5, NT ], [6 8, NT ] NT NT, 1 Sprague2Dawley, g, (112 g/ kg),,, 011 %, (PT26, ), ( ) (, BP), XDH23B ( ) (HR) 38 [9 Noble ], 115 mm mm, 018 mm mm, 502,

2 : 141 (NT, Sigma ), ( ), ) ( ) (CSF), ph 7 ( Pfizer ) 0101 mol/ L HCl CSF, NaOH ph 7, 3 l, 1 min CSF, ph 3 NT, NT, NT 1 h NT 10 20 g, 3 l 5 %,,, NT, ( x s x ), t 2 211 ( icv) NT BP HR NT 1 20 g, 1

2 2 : 141 (NT, Sigma ), ( ), ) ( ) (CSF), ph 7 ( Pfizer ) 0101 mol/ L HCl CSF, NaOH ph 7, 3 l, 1 min CSF, ph 3 NT, NT, NT 1 h NT g, 3 l 5 %,,, NT, ( x s x ), t ( icv) NT BP HR NT 1 20 g, g,, ( ) ( ) ( ) ( ) ( ) kpa ( n = 6) ;, ( ) ( ) ( ) ( ) ( ) kpa ( n = 5), 20 g, icv NT NT ( 1 h), 14 BP ( ) kpa, HR ( ) bpm icv, 14 icv CSF 3 l, BP HR [BP ( ) kpa, HR ( ) bpm ] NT icv NT (10 g) NT, s, 3 5 min, ( ) kpa NT BP min HR 1 min, NT (10 g) Fig. 1 Changes in blood pressure in re2 sponse to NT (10 g) injected in2 to the lateral cerebroventricle Injection of NT induced a rise ( ) or a drop followed by a rise ( ) of blood pressure. : injection of NT.

3 min, HR ( ) bpm HR min NT (10 g) Fig. 2 Cardiovascular responses induced by injection of NT (10 g ) into the lateral cerebroventricle : pressor response ( n = 15) ; " : depressor response ( n = 12) ; and : control group ( n = 14). : injection of NT of CSF. 3 P < 0105, 3 3 P < 0101, P < vs CSF group NT 12, icv NT (10 g) s, BP, 6 10 min, ( ) kpa, NT BP min HR BP 3 4 s, 7 15 min, ( ) bpm, min ( 2, 3), 3 icv NT (10 g), BP ( 1, 3), HR 16, icv NT (20 g), 7 BP [ ( ) kpa ], 7 BP [ ( ) kpa ], 2 BP, 3 HR [ ( ), ( ) 70 bpm], 20 g NT 10 g NT 5, icv NT (10 g), BP HR, icv NT (10 g), NT NT 212 NT ,icv NT (10 g), 1 (115 g) min, NT(10 g),

4 2 : NT (10 g) Fig. 3 Changes in blood pressure in response to NT (10 g) injected into the lateral cerebroventricle Injection of NT induces a drop ( )or a rise followed by drop( ) of blood pressure. I : injection of CSF ; : injection of NT. ( 1) 60 min icv NT (10 g), NT 4, icv NT (10 g), NT NT, NT , icv NT (10 g), (215 g) min icv NT (10 g), ( 1) 60 min icv NT (10 g), NT 4, icv NT (10 g), NT, M NT, NT , icv NT (10 g), 5 icv NT (10 g), H 1 (50 g) min,, NT (10 g) 1, NT NT H NT (10 g) (6 ) NT (10 g) (5 ), H 2 (250 g) 9 10 min,, NT (10 g), NT ( 1) NT H 2

5 (10 g, icv) Table 1 Effects of various blockers on the cardiovascular responses induced by neurotensin Treatment (NT) (10 g, icv) n Maximal change in BP / kpa Before inj1 After inj1 Maximal change in HR / bpm Before inj1 After inj1 Prazosin Atropine Chlorpheniramine BP group BP group Cimetidine BP group BP group P < vs before injection., icv NT (20 g), icv NT (20 g) NT (10 g), 3, NT, NT 10 g 20 g, 1 NT ; M NT NT, 1 ; M,,, NT,, [10 Ishizuka ], NT (iv), (iv) 1 NT NT [11 Ciriello ], NT ; M, NT NT [4 Shido ], NT, NT, NT,, NT

6 2 : 145 [12 Kubo ], NT NT, NT, icv NT,, NT,,, NT SR48692 SR142948A, [13, 14 NT, ], NT NT Quirion Rioux [15 ], NT 52 H 1 H 2 NT, NT, [1 ] Rioux F, Quirion R, St2Pierre S, et al. The hypotensive effect of centrally administered neurotensin in rats. Eur J Pharmacol, 1981, 69 : [2 ] Quirion R, Rioux F, St2Pierre S, et al. Hypotensive effects of centrally and peripherally administered neuro2 tensin and neurotensin derivatives in rats. Neuropeptides, 1981, 1 : [3 ] Sumners C, Phillips MI, Richards EM. Central pressor action of neurotensin in conscious rats. Hypertension, 1982, 4 : [4 ] Shido O, Nagasaka T. Effects of intraventricular neurotensin on blood pressure and heat balance in rats. Jap J Physiol, 1985, 35 : [5 ] Higgins GA, Hoffman GE, Wray S, et al. Distribution of neurotensin2immunoreactivity within baroreceptive por2 tions of the nucleus tractus solitarius and the dorsal vagal nucleus of the rat. J Comp Neurol, 1984, 226 : [6 ] Young WS, Kuhar MJ. Neurotensin receptor localization by light microscopic autoradiography in rat brain. Brain Res, 1981, 206 : [7 ] Kessler JP, Moyse E, Kitabgi P, et al. Distribution of neurotensin binding sites in the caudal brainstem of the rat : A light microscopic radioautographic study. Neuroscience, 1987, 23 : [8 ] Kessler JP, Beaudet A. Association of neurotensin binding sites with sensory and visceromotor components of the vagus nerve. J Neurosci, 1989, 9 : [9 ] Noble EP, Wurtman RJ, Axelrod J. A simple and rapid method for injecting H 3 2norepinephrine into the lateral ventricle of the rat brain. Life Sci, 1967, 6 : [10 ] Ishizuka T, Wei X, Kubo T. Cardiovascular effects of microinjections of thyrotropin2releasing hormone, oxytocin and other neuropeptides into the rostral ventrolateral medulla of the rat. Arch Int Pharmacodyn Ther, 1993, 322 : [11 ] Ciriello J, Zhang TX. Cardiovascular effects of neurotensin microinjections into the nucleus of the solitary tract. Brain Res, 1997, 749 : [12 ] Kubo T, Kihara M. Modulation of the aortic baroreceptor reflex by neuropeptide Y, neurotensin and vasopressin microinjected into the nucleus tractus solitarii of the rat. Naunyn Schmiedebergs Arch Pharmacol, 1990, 342 : [13 ] Nisato D, Guiraudou P, Barthelemy G, et al. SR48692, a non2peptide neurotensin receptor antagonist, blocks the cardiovascular effects elicited by neurotensin in guinea pigs. Life Sci, 1994, 54 : PL

7 [14 ] Schaeffer P, Laplace MC, Bernat A, et al. SR142948A is a potent antagonist of the cardiovascular effects of neurotensin. J Cardiovasc Pharmacol, 1998, 31 : [15 ] Quirion R, Rioux F, Regoli D, et al. Compound 48/ 80 inhibits neurotensin2induced hypotension in rats. Life Sci, 1980, 27 : Acta Physiologica Sinica Apr. 1999, 51 (2), EFFECT OF INTRACEREBROVENTRICULAR NEUROTENSIN INJECTION ON BLOOD PRESSURE IN RAT ZHANG XIAO2HUI, YIN GUO2XIANG, NI HUI ( Department of Physiology, Nanjing Medical University, Nanjing ) ABSTRACT Injection of neurotensin (NT) (10 and 20 g/ rat) into the lateral cerebroventricle (icv) could induce a rise or a drop of blood pressure and a slowing down of heart rate in urethane anaesthetized male rats (112 g/ kg). The central pressor and depressor response to NT could be respectively blocked by pretreatment with 1 2receptor blocker prazosin (115 g/ 3 l, icv) and M receptor blocker atropine (215 g/ 3 l, icv). No significant changes in central cardiovascular responses to NT were observed following pretreatment with either H 1 receptor blocker chlorpheniramin (50 g/ 3 l, icv ) or H 2 receptor blocker cimetidine (250 g/ 3 l, icv). In view of the above results it appears that NT, cate2 cholamine and acetylcholine are all involved in central regulation of blood pressure. Key words : neurotensin ; blood pressure ; prazosin ; atropine ; intracerebroventricular injection

Cardiovascular Physiology IV.

Cardiovascular Physiology IV. 48. Short-term control mechanisms of arterial blood pressure. 49. Long-term control of arterial blood pressure. Ferenc Domoki, November 14 2017. Challenges/expectations Blood