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1 , , 51 (4), Acta Physiologica Sinica 3 (, ) 30 2 (CSN), 39 (NA) : (1) (MTS), imp/ s ( P < 01001) ; (2), imp/ s ( P < 0101) ; (3) imp/ s ( P > 0105), 10-6 mol/ L MTS, ; (4) imp/ s, 10-6 mol/ L MTS, ; (5) imp/ s ( P < 01001),,, imp/ s ( P < 01001) ; (6) 7, imp/ s ( P < 0101),,, imp/ s imp/ s ( P > 0105) : (1) NA, NA ; (2), 2,, CSN, : ; ; ; ; : Q438 ; R (CB) ( ) (CA), (DA) (NA) [112 ] DA NA [3 ], NA,, NA, [4 ], CB, PO 2 PCO 2 ph, CSN, NA CSN, 1 2 CSN, NA (No ) Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

2 ,, kg 25 % ( g/ kg),,, CSN, CSN, CSN CSN, CB CSN, 100 % MTS, 4 4 h, 95 % O 2 25 % CO 2 MTS [MTS (mmol/ L) : NaCl 112, KCl 4169, CaCl , MgCl 2 115, Na glutamate 42, glucose 515, HEPES 4199 ], ( IBM2PC, 450D 3 2),, (ABL230) PO 2 PCO 2 ph : (1) : 95 % O 2 25 % CO 2 MTS (PO kpa, PCO kpa, ph , ) 1, 95 % N 2 5 % CO 2 MTS 5 min (PO kpa, ),,, : (2) (Sigma1N5785) : ( kpa ), NA MTS 5 min (3) (prazosin, Praz ; ) :, Praz MTS 5 min (4) (yohimbine, Yoh ; ) :, Yoh MTS 5 min 113 x s, t 2 211, imp/ s, imp/ s ( P < 0101, 1B, 1) mol/ L NA 5 min, imp/ s imp/ s ( P < 0101, 1) mol/ L NA MTS 5 min,, ( 2) Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

3 4 : Fig11 Unit discharges recorded from chemosensory afferent fibers of carotid2sinus nerve preparation in the rabbit A, C, E1 Control1 B1 Hypoxia1 D1 Hypoxia + yohimbine. F1 Hypoxia + no2 radrenaline , 10-6 mol/ L Praz MTS, ( P > 0105, 1) mol/ L Praz MTS 5 min, 10-6 mol/ L Praz MTS 5 min, ( P < 01001, 2) 214 6, 10-6 mol/ L Yoh MTS, ( P < 0105, 1) mol/ L Yoh 5 min 10-6 mol/ L Yoh MTS 5 min, ( 1D, 2) Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

4 Table 1 Effects of noradrenaline, prazosin and yohimbine on the unit dis2 charge of carotid chemoreceptor Table 2 Effects of prazosin, yohimbine and nor2 adrenaline on the unit discharge of carotid chemoreceptor during hypoxia n Unit discharge / imp s - 1 Control Hypoxia Control Noradrenaline Control Hypoxia Control Prazosin Control Hypoxia Control Yohimbine P < 01001, 3 3 P < 0101, and 3 P < 0105 vs control. n Unit discharge / imp s - 1 Control Hypoxia Control Hypoxia + noradrenaline Control Hypoxia Control Hypoxia + prazosin Control Hypoxia Control Hypoxia + yohimbine P < 01001, 3 3 P < 0101, and 3 P < 0105 vs control1 3,,, PO 2, NA CSN, NA CSN NA 1 2 Yoh, CSN, PO 2, CSN NA, Yoh CSN, Yoh NA, NA, CSN NA CSN 2, 2, Yoh 2 NA [6 ] [6 Almaraz ], CB 2, 2 Yoh PO 2 ( kpa) [ H 3 ]NA 100 % 53 % ;, Yoh, CSN ( ), NA Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

5 4 : 411, CSN NA, NA,,, NA MTS,, NA ( [7 1F), ], Ryan [8 ],, PO 2, NA ( 56 %) ;, NA, ;, [ 1 ] Mir AK, McQueen DS, Pallot DJ, et al. Direct biochemical and neuropharmacological identification of dopamine D22receptors in the rabbit carotid body. Brain Res, 1984, 291 : [2 ] Folgering H, Ponte J, Sadig T. Adrenergic mechanisms and chemoreception in the carotid body of the cat and rabbit. J Physiol, 1982, 325 : [3 ] Gonzalez C, Lopez2Lopez JR, Obeso A, et al. Cellular mechanisms of oxygen chemoreception in the carotid body. Respir Physiol, 1995, 102 : [4 ] Pang L ( ), Miao ZH ( ), Wang YL ( ) et al. Effects of different natural stimulants on the activity of the rabbits carotid chemoreceptor. Acta Physiol Sin ( ), 1996, 48 : (in Chinese with English abstract). [5 ] Iturriaga R, Lahiri S, Mokashi A. Carbonic anhydrase and chemoreception in the cat carotid body. Am J Physi2 ol, 1991, 261 : C565 C573. [ 6 ] ALmaraz L, Perez2Garcia MT, Gomez2Nino, et al. Mechanisms of alpha222adrenoreceptor mediated inhibition in rabbit carotid body. Am J Physiol, 1997, 272 : C628 C637. [7 ]..,, :. :, 1995, [8 ] Ryan ML, Hedrick MS, Pizarro J, et al. Carotid body noradrenergic sensitivity in ventilatory acclimatization to hypoxia. Respir Physiol, 1993, 92, Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

6 412 Acta Physiologica Sinica Aug. 1999, 51 (4), HYPOXIA2IND UCED INCREASE IN NERVE ACTIVITY OF RABBIT CAROTID BODY MEDIATED BY NORADRENALINE 3 PANG L EI, MIAO ZHI2HUI, DONG L I, WANG YAN2L ING ( Department of Physiology, Hebei Academy of Medical Sciences, Shijiazhuang ) ABSTRACT The responses of 39 hypoxia2sensitive units of chemoreceptive afferent in sinus nerve to noradrenaline ( NA) and its antagonist were recorded in carotid body2sinus nerve preparations from 30 rabbits. The results are as follows. (1) Discharges of the units in2 creased from to imp/ s ( P < 01001, n = 19) upon lowering PO 2 of modified Tyrode solution. (2) Adding NA to the perfusate led to an increase in the unit discharge from to imp/ s ( P < 0101, n = 19). (3) Prazosin did not alter the basal frequency of chemosensory u2 nit discharges under normoxic conditions ( P > 0105, n = 4). (4) Yohimbine (10-6 mol/ L) did not alter the basal frequency of chemosensory unit discharges under normoxic conditions ( P > 0105, n = 6). (5) Chemosensory responses to hypoxia were not altered after pretreatment with prazosin. (6) Chemosensory responses to hypoxia were inhibited by pretreatment with yohimbine. The present results suggest that (1) NA is not mainly concerned with spontaneous discharges of chemoreceptor sensitive to hypoxia, but does elicit an increase in spontaneous discharges, and (2) the increase of chemosensory unit discharges produced by hypoxia can be inhibited by yohimbine. It is likely that the exci2 tatory action of hypoxia on chemoreceptive process is mediated by NA. Key words : carotid body ; unit discharge ; hypoxia ; noradenaline ; yohimbine 3 Supported by the National Natural Science Foundation of Hebei Provine (No ) Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

Effects of muscle spindle afferents on the regulation of respiration in rabbit 3

Effects of muscle spindle afferents on the regulation of respiration in rabbit 3 , Dec. 2000, 52 (6) : 491 496 491 Acta Physiologica Sinica 3 3,,, (, 710061) : 63, (RRN) (Phr1D), (Sch) : (1) Sch, (Ti), (Te), Ti/ Te (RF), ; Te, Ti, Ti/ Te, RF, (2) Sch (37/ 56), (10/ 17), (3) Sch, 1