McMASTER UNIVERSITY DEPARTMENT OF BIOCHEMISTRY Biochemistry 3N3 - NUTRITION AND METABOLISM 2004 (Term II)
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1 McMASTER UNIVERSITY DEPARTMENT OF BIOCHEMISTRY Biochemistry 3N3 - NUTRITION AND METABOLISM 2004 (Term II) Course Instructors: Stephanie A. Atkinson, Ph.D. Professor in Pediatrics, Associate Member, Biochemistry HSC 3V42, Ext , satkins@mcmaster.ca Michelle MacDonald, Ph.D. Assistant Professor, Biochemistry HSC 4H24B, Ext , macdonml@mcmaster.ca Teaching Assistants: Patricia Collins, x 22135, Room 3H25, collip@mcmaster.ca Tyler Watt, x 22734, Room 4N71, watttk@mcmaster.ca Course Description: The objectives of this course are to build on the basic knowledge of metabolic pathways and their regulation as learned in previous biochemistry courses, and to integrate this knowledge into the study of nutritional biochemistry, how nutrients influence gene expression, and the relevance of specific nutrients to the prevention and treatment of common human diseases. Drug-nutrient and nutrient-nutrient interactions important to metabolism will be covered. Perspectives on the subject matter will evolve from the cellular and molecular level to whole body integration of metabolism. Opportunities will be provided for independent study, knowledge evaluation and self-selection of course content. NOTE: You do not attend class on Independent Study Days but instead complete the Independent Study Assignments provided to ensure that you have the background knowledge expected as prerequisite for the new course material. Times: Mon. 9:30-10:20 Place: HSC 1A1 Tues. 10:30-11:20 Thurs. 9:30-10:20 Recommended Text: Reference Texts: - Groff JL and Gropper SS (eds). Advanced Nutrition and Human Metabolism. Canada: Wadsworth/Thomson Learning 3 rd ed (diagrams used in teaching will be primarily from this textbook) Voet D and Voet JG. Biochemistry. 2 nd Edition John Wiley & Sons Inc., N.Y Voet D, Voet JG, Pratt CW. Fundamentals of Biochemistry. John Wiley & Sons Inc., NY a list is attached of Figures and Tables from these texts that will be used in the lectures and for which you are responsible for tests. 1
2 Reference Materials: 1. Course Outline will be on the Website for Department of Biochemistry: - Independent study assignments, problems for tutorials and lecture outlines will be accessible through learnlink@mcmaster.ca 2. BCH 3N3 Binder will contain answers and marking schemes for Term test and special review papers if used as resources for lectures. (2 copies ON RESERVE in library). 3. CDs on Nutrition available for use in HSC Library (A/V section). - McNUTrition (developed at McMaster) - Nutrition in Medicine Series (Univ. Of North Carolina at Chapel Hill) - the CDs on Nutritional Anemias, Diet, Obesity and Cardiovascular Disease and Nutrition in Cancer will be most useful for the course content. 4. WEBSITES with Reliable Information on Nutrition Office of Nutrition Policy and Promotion, Health Canada Centers for Disease Control & Prevention - National Institute of Nutrition (Canada) - Tufts University Nutrition Navigator - American Journal of Clinical Nutrition - U.S. Government Food and Nutrition Information Centre - Mark Allocation: EVALUATION 1. Mid-term test 1 (Feb 12) Lectures 1-16 = 25% 2. Assignment (given out Feb.23 - due for hand-in = 25% and presentation April 1 st ) 3. Final Exam - Lectures 1-36 = 50% For final marks, percentage grades will be rounded to the nearest integer and converted to a final letter grade using the conversion scale in the undergraduate calendar. You will be informed if any changes to the evaluation format become necessary. Assignment: Critical Appraisal of Current Topic in Nutrition The assignment will consist of a critical appraisal of the current scientific literature on a topic of interest related to nutrition and metabolism. A choice of selected papers to review will be provided. Guidelines for critical appraisal of a scientific paper can be found in: (Groff/Gropper 3 rd edition, Chapter 17, Experimental Design and Critical Interpretation of Research). This chapter is recommended reading prior to starting the assignment. The class will be divided into small groups each with a preceptor assigned (either one of the 2
3 professors or Teaching Assistants). Group work on the assignment will take place via Learn Link. The paper and presentation will be a group effort. Students will be marked for participation on Learn Link. Further details of the assignment will be discussed in class. Self-Evaluation: 1) Assignments for Independent Study Days are provided for you to consolidate your previous knowledge of nutritional biochemistry prior to delving into the more applied topic areas that will be covered in the lectures. The assignments for each Independent Study Day will be available on Learnlink. 2) A Problems Tutorial will be held on two occasions. Problem sets will be available in advance of the Tutorial on LearnLink. The Problem Questions provided will help you to self-test your understanding of the course material and to practice for the type of exam questions used in this course. To obtain the greatest benefit from these exercises, you must prepare answers to the problems and then attend the class in which the Teaching Assistants will review the answers. The Problems Tutorial will also be an opportunity to review the Independent Study assignments if you have questions. University Policy Exams and Academic Dishonesty 1. You must bring student I.D. to all tests/exams. 2. Absence from a test or exam will result in an automatic zero unless evidence can be provided for medical or legal conflicts. If you know ahead of time that you re going to be absent during a time when a test is scheduled then you must notify the REGISTRAR S OFFICE as soon as possible. 3. You may use a non-programmable calculator during any of the tests/exams. 4. ACADEMIC DISHONESTY: Please refer to the Statement on academic ethics and the Senate resolutions on academic dishonesty found in the Senate policy statements as distributed by the Senate office. Plagiarism of your classmate s work (be it from this year or previous years), or any text from books, the web, tests or papers, is unacceptable conduct and is subject to penalty if observed by the course professor or assistants. 3
4 Biochemistry 3N3 Lecture Schedule - January - April 2004 * NOTE: You do not attend class on days noted by a * but instead complete Independent Study Assignments which are posted on LearnLink on the Biochemistry Website. Chapters - Groff Date Lecture Title Chapters Lecturer A. Introduction Jan.5 1 The Digestive System: An overview Groff-1 & S.Atkinson Dietary Reference Intakes Notes on S.Atkinson - derivation and application Learn Link B. Carbohydrate Metabolism * 8 3 Independent Study #1: Voet/Groff Independent Review of classification of carbohydrates & proteins Study and metabolic pathways for carbohydrates & proteins 12 4 Carbohydrate digestion and mucosal transporters Groff- 2 & 4 S.Atkinson 13 5 Insulin action and glucose transporters Groff-4 S.Atkinson 15 6 Origins of obesity and metabolic syndrome Groff-8 S.Atkinson (P. 242) & notes on Learn Link C. Protein Metabolism 19 7 Digestive enzymes & membrane transport Groff-7 S.Atkinson systems for protein 20 8 Functions & metabolic defects of specific Notes on S.Atkinson amino acids Learn Link 22 9 Diet, neurotransmitters & behaviour Groff- 16 S.Atkinson (p ) 4
5 D. Lipid Metabolism Independent Study #2 Groff-6 Independent Classification of lipids and nomenclature Voet, et al Study of fatty acids Cholesterol Metabolism Groff-6 M.MacDonald Fat digestion and absorption Groff-2 & 6 Feb.2 13 Lipoproteins classification, Groff-6 function & apolipoproteins 3 14 Lipoproteins and lipid transport Groff Essential Fatty Acids & Eicosanoids: Groff-6 biochemistry and function 9 16 Lipids & cardiovascular disease risk Groff Problems Tutorial: The Independent Study Assignment #1 & #2 and Problems set #1 will be reviewed P. Collins T. Watt TEST #1 (50 minutes) includes to end of lecture #16 READING WEEK FEBRUARY 16-21, 2004 E. Vitamins Feb Orientation to assignment S.Atkinson M.MacDonald P.Collins Group meetings for assignment T.Watt Vitamins: Biochemistry, Function, and Groff 9 & 10 M.MacDonald Pharmacology 5
6 Mar.1 22 Vitamins as Antioxidant Nutrients: Generation Groff 9 & 10 of reactive oxygen species ( p ) 2 23 Vitamin Action in elimination of reactive Groff 9 & 10 oxygen species Vitamins C and E Mar.4 24 Folate, B 12, homocysteine & cardiovascular Notes on Learn Link disease Groff 9 Mar Selected topics in Nutrition (student choice) TBA 9 26 Selected topics in Nutrition (student choice) TBA F. Vitamin D and Macrominerals Mar Vitamin D: A steroid hormone Groff 10 S.Atkinson Gene defects in vitamin D metabolism Notes on S.Atkinson Learn Link Ca, P and Mg: Regulation of Metabolism Groff 11 S.Atkinson Osteoporosis: nutrition s role in prevention/ Groff 11 S.Atkinson treatment & 15 (p ) G. Trace Elements Case studies on Iron Metabolism Notes on Learn Link S.Atkinson Trace elements I: Regulation of iron Groff 9 & 12 S.Atkinson absorption & gene expression of iron binding ( p ) proteins Trace elements II: Assessment of iron status Groff 12 S.Atkinson Trace elements III: Iron deficiencies and Groff 12 S.Atkinson toxicities H. Assignment Presentations & Review Assignment Presentations Atkinson, McDonald Watt & Collins 6
7 Apr Assignment Presentations Atkinson, McDonald Watt & Collins 5 37 Problems Tutorial lectures END OF CLASSES - April 5, Final exam for BCH 3N03 will be three (3) hours duration. Biochemistry 3NO FIGURES and TABLES For the lectures, many of the Figures and Tables from the recommended textbooks will be used to demonstrate the key information regarding metabolites of nutrients in the body and disease processes. The following list provides the Figure and Table numbers from the textbooks that will be used in each section of the course. It would be helpful if you reviewed this material in advance or brought a copy to class to follow. Groff & Gropper Voet & Voet 3 rd Edition (1999) 2 nd edition Introduction Fig. 2.1, 2.4, 2.5, 2.6, 2.8, 2.9, 2.20, 2.21 Table 2.2 (part) Carbohydrate Fig. 1.17, 2.18, 4.3, 4.4, 5.2 Fig Metabolism Pg. 242 (Fig. 1) 12.17, Table 4.2 Protein Metabolism Fig. 2.19, 7.5, 7.6, 7.7, , 7.22, , 7.30, 7.39, 7.40, 16.1, 16.A, 16.2, 16.4 Table 7.2,
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