Bacillus subtilis Fusarium oxysporum f. sp. radicis-cucumerinum. B. subtilis F. oxysporum f. sp radicis-cucumerinum

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1 .Fusarium oxysporum f. sp. radicis-cucumerinum 3 * 3 (90/3/ : - 9// : ) (339-35) 390 Fusarium oxysporum f. sp. radicis-cucumerinum. F. oxysporum f. sp radicis-cucumerinum ( -) F. oxysporum f. sp.. radicis- cucumerinum Fusarium : oxysporum f. sp. radicis-cucumerinum. yousefi.ariya@gmail.com : : *

2 (SAR).(Durrant & Dong, 00). SAR.(Ryals et al., 996) (ISR) (SAR).(Pieterse et al., 00) ISR SAR.(Pieterse et al., 00) (SA) (Kessmann et al., 99; Vallad & Goodman, 00) (Murphy et al., 999 ; Naylor et.al., 99) (Prithiviraj et al., 005). (Ryals et al.,.996) Murphy et al. (000). Raju et al. (00) (L550) (ICC0).. 0/ 0/5...(Fayza & Sabrey, 006) (GE) Glomus etunicatum. (Fol) F. oxysporum f. sp lycopersici GE. GE 0. GE. GE (Ozgonen et al.,.00) (PGPR)

3 3... :.(Shahriyari & Zare, 00).... F. oxysporum f. sp. radiciscucumerinum.. :::.. ± C ) (. p Fusarium oxysporum f. sp. radiciscucumerinum (Kloepper et al.,.90) (Pieterse et (ISR) ).al., 00) ( PGPR (PGRS). PGPR.(Kloepper et al., 99) PGPR..(Kurata, 99) B. pumilis. ( ) Cercospora beticola (BACJ ) B. mycoides (Bargabus et al., 00; Bargabus Fusarium oxysporum.et al., 00) Schlechtend.: Fr. f. sp. radicis-cucumerinum D.J.Vakalounakis (Vakalounakis, 996; Punja & Parker,.000; Moreno et al., 00)

4 /5.(Ricker, 963) /5 :::.. ) 9 (.. NB (0 9 CFU/ml) 590nm (OD= 0.06).(Thompson, 996) -3.. (SA) (Merck) 00 %96.(Oka et al., 999) 00 NA 9. ).( PDA N=A-B/A. =A B = =N (Etebarian et.al., 005). SA : -

5 33... : ( ) Fusarium oxysporum f. sp. radicis-.cucumerinum (Vakalounakis, 996) 0 3 = = = =. =.(PAL) 00 6/ ph 0/ (Sahebani, 00) (PAL) 0/. 0 0/ 0/ 0/. - :. - ( ) - ( ) ( ) - - ( ) ) () ( ) ( p.. ().. 5 SAS (9.0). : (0 9 CFU/ml)

6 390 3 %. 5.(3) F. oxysporum f. sp. - radicis-cucumerinum (Mm) (%) 3/35C /59C 6/55C /95B 00A 00A 00A Cv=.05 (mm) SA % ( ). %..() F. oxysporum f. sp. radicis-cucumerinum F. o. r. c. 5.( ) ( 3 ) 5 %00 ( 6).( ) ( ) % ). (. 5

7 35... : C A C B 5 - () F. oxysporum f. sp. radicis-.(a-c) cucumerinum 5-3 (cm) 3/69bc 3/ab /95c 3/353bc /990bc /3a (g) /9b 3/30ab /c /90b /6b 3/66a (g) / a /0 a 0/5b /0 a /59 a / a -936 a -a /533 a /533 a /936 a 0/0B P+F+B P+F+SA sp (5mM)+B P+F P+F+SA sd (5mM) P+F+SA sd (5mM)+B (. ) % =SA sd =SA sp =B CO =F =P. =co - (cm) /035a /5636b /559b /699ab /660ab /6393ab (g) /69a /03a /5a /653a /66a /3a (g) /5a /953a /0a /9a /63A /B 0/0d /ab /0bc /0c /036c /53a CO P+SA sd (5mM)+F P+SA sd (5mM)+B+F P+ SA sp (5mM)+B+F P+B+F (. ) % =SA sd =SA sp =B =F =P. =CO P+F 3.

8 ).(5-5 (g) (g) (cm) P+B+F /036bc /055a /9a /35ab P+SA sd (mm)+b+f /0c /600ab /560a /305ab P+SA sp (mm)+b+f 0/5d /636ab /9a /39a P+SA sd (mm)+f /ab /0c /6305a 3/56cd P+SA sd (3mM)+F /93a /06c 0/363c 3/60bcd P+F /53a /0c 0/093c 3/653d CO 0/0e /55b /9b 3/935abcd P+SA sp (mm)+b+f * /0c /90ab /6903a /359ab P+SA sd (3mM)+B+F ** /30abc /5B /533a /00abc % ).( =P =F =B SA sp = =SA sd =CO * ** = F. oxysporum f. sp. radicis-cucumerinum. ) (

9 : Fusarium oxysporum f. sp. radicis-cucumerinum. ±.. =sa) =b =sb =co =f.(. 00.(Yu & Zheng, 006) SA ) ( ) (.. (SAR). (ISR).(Van Loon et al., 99).

10 (ISR).(Van Loon, 00).. PGPR. 6. 0/ F. oxysporum f. sp lycopersici 0/6.(Ozgonen et al., 00) ph SA ) ( ) (.... PAL.

11 : % PAL -- Seah et al. (996) ) ( ) ( 0 0/ SA. 0 0/ 0/5. SA PAL POX SA Ggt. 00 Fusarium oxysporum f. sp lycopersici (Fol). 6 5/9 3/.(Sudhamoy et al., 009) W 6 Alternaria alternata.(chandrasekaran et al., 009) Fusarium oxysporum f. sp ciceri.(saikia et al., 006)

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