Funkcje soli żółciowych w innych niż lipolityczne procesach trawienia

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Frederica Newman
5 years ago
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1 Funkcje soli żółciowych w innych niż lipolityczne procesach trawienia Adam Macierzanka Politechnika Gdańska, Wydz. Chemiczny Katedra Technologii Koloidów i Lipidów

2 1. Structuring of food colloids, proteins, lipids FOOD EMULSION, GEL (gelation, adsorption / emulsification, enzymatic crosslinking, heat treatment 2. Digestion of food (in vitro models, in vivo, interactions with physiological surfactants) 3. Transport of food (interaction of post-digested particles with the small intestinal mucus, ex vivo tissue, bioavailability) Intestinal mucus Epithelium

3 Sole żółciowe (bile salts) Maldano-Valderrama J., Wilde P., Macierzanka A., Mackie A.R. (2011) The role of bile salts in digestion. Advances in Colloid and Interface Science,

4 Interfaces & Lipid Digestion Lipase Breakdown products Lipase Colipase Aqueous Phase Oil Phase Triglyceride Phospholipid Bile salt Triglyceride Oil drop

5 Lipase Breakdown products Lipolysis in the small intestine lipid drop Aqueous phase FA, MG Lipid phase TG polar lipids fatty acids protein bile salts lipase co-lipase mixed micelle

6 Lipolysis (triglyceride droplet under confocal microscope) Norwich, UK

7 Absorption of Fats/Lipids (Triglycerides) Copyright Rozaini Othman

8 Rola SOLI ŻÓŁCIOWYCH w trawieniu białek

9 SDS-PAGE analysis of dietary proteins that were exposed to trypsin and chymotrypsin in the presence or absence of conjugated bile acids. For each protein, the following treatments were performed: (a) protein control in 20 mmol/l sodium phosphate, ph 7 (no incubation); (b) protein treated with 0.1 mg/ml trypsin and chymotrypsin at 37 C for 15 minutes; (c) protein treated with 0.1 mg/ml trypsin and chymotrypsin and 10 mmol/l physiologic bile acid mixture at 37 C for 15 minutes. Effect of Bile Salts on Protein Digestion Gass et al., GASTROENTEROLOGY 2007

Bile Salts conc. -Lactoglobulin (BLG) Gass et al., GASTROENTEROLOGY 2007 Effect of conjugated bile acid concentration on proteolysis of BLG. BLG was incubated with 0.

10 Bile Salts conc. -Lactoglobulin (BLG) Gass et al., GASTROENTEROLOGY 2007 Effect of conjugated bile acid concentration on proteolysis of BLG. BLG was incubated with 0.01 mg/ml trypsin and chymotrypsin and increasing levels of physiologic bile acid mixture (0 14 mmol/l). All mixtures were incubated at 37 C for 15 minutes before heat deactivation and application onto SDS- PAGE.

11 Maldano-Valderrama J., Wilde P., Macierzanka A., Mackie A.R. (2011) The role of bile salts in digestion. Advances in Colloid and Interface Science,

12 1. Structuring of food colloids, proteins, lipids FOOD EMULSION, GEL (gelation, adsorption / emulsification, enzymatic crosslinking, heat treatment 2. Digestion of food (in vitro models, in vivo, interactions with physiological surfactants) 3. Transport of food (interaction of post-digested particles with the small intestinal mucus, ex vivo tissue, bioavailability) Intestinal mucus Epithelium

13 Small intestinal mucus: SAMPLE COLLECTION Porcine small intestine Mucus gently removed from the mucosal surface Mucus mixed up with enzyme inhibitors and antimicrobial agents; stored at -80C

CONFOCAL MICROSCOPY (diffusion of particles) Ensemble mean-squared displacements (MSD) +BS ζ-potential -50 mv Particulate covered with bile salts (high negative charge) - - - - - - - -

14 CONFOCAL MICROSCOPY (diffusion of particles) Ensemble mean-squared displacements (MSD) +BS ζ-potential -50 mv Particulate covered with bile salts (high negative charge) BS ζ-potential -15 mv Diffusion in the mucus was driven by high negative charged provided by bile salts (BS) adsorbed to latex beads A. Macierzanka et al., Soft Matter (2011)

Small intestinal mucus: SAMPLE COLLECTION HUMAN

gently from the mucosal surface of distal small

Dr Łukasz Krupa, Dr Robert Staroń, Prof.

15 Small intestinal mucus: SAMPLE COLLECTION HUMAN small intestine COLONOSCOPY Mucus aspirated gently from the mucosal surface of distal small intestine 50 healthy volunteers aged yrs Dr Łukasz Krupa, Dr Robert Staroń, Prof. Krzysztof Gutkowski Klinika Gastroenterologii i Hepatologii

16 Digesta penetration into small intestinal mucus Digestion of yogurt Olivia MÉNARD, Didier DUPONT (INRA-Agrocampus Ouest, UMR 1253, Rennes)

17 Digesta penetration into small intestinal mucus Digestion of yogurt Gastric compartment: pepsin, fungal lipase Intestinal compartment: porcine pancreatin, porcine bile extract Olivia MÉNARD, Didier DUPONT (INRA-Agrocampus Ouest, UMR 1253, Rennes)

18 Digesta penetration into small intestinal mucus Digestion of yogurt Samples of yogurt digesta withdrawn form the intestinal vessel after: 1h, 2h, 3h, 4h and 4.5h Before After

19 Digesta penetration into small intestinal mucus Time-lapse confocal microscopy Mucus Prof. Alan R. MACKIE 500 um Mucus boundary Digesta t = 0

20 Digesta penetration into small intestinal mucus Time-lapse confocal microscopy Mucus 500 um Digesta Time 90 min

21 Podsumowanie Sole żółciowe wspomagają kinetykę trawienia białek (in vitro). Sole żółciowe są niezbędne do efektywnego transportu substancji pokarmowych ze światła jelita cienkiego do powierzchni warstwy nabłonkowej jelita, poprzez ochronną warstwę śluzową pokrywającą nabłonek.

Considering whether improved knowledge of how we digest fat can help reduce its impact on health Professor Pete Wilde

Considering whether improved knowledge of how we digest fat can help reduce its impact on health Professor Pete Wilde Institute of Food Research, Norwich, UK Evolution of the modern diet Structurally rich