RELATIONSHIP OF HOMOCYSTEINE AND ITS METABOLIC ENZYME GENES POLYMORPHISMS WITH ESSENTIAL HYPERTENSION IN MONGOLIANS

Transcription

1 377 A Hcy PCR-RFLP MS A 2756 G Hcy Hcy Hcy P<0.05 MS A 2756 G P>0.05 MS A 2756 G Hcy R544.1 A DOI: /j.cnki.issn x X RELATIONSHIP OF HOMOCYSTEINE AND ITS METABOLIC ENZYME GENES POLYMORPHISMS WITH ESSENTIAL HYPERTENSION IN MONGOLIANS JIA Hai-yu CAO Zhong-chao LI Chang-qing Department of Health Affiliated Hospital Inner Mongolia Medical University Hohhot China Abstract Objective To investigate the relationship between the plasm homocysteine Hcy level as well as methionine synthase A 2756 G MS A 2756 G polymorphisms and essential hypertension in Mongolians.Methods A total of 165 Mongolian pations with essential hypertension Mongolian hypertension group and 150 Mongolian normal controls control group were detected the gene polymorphisms of methionine synthase A 2756 G by polymerase chain reaction - restriction fragment length polymorphism technique.the level of plasma Hcy was measured by enzymoimmunoassay.result The plasma Hcy s lever in hypertension group was significantly higer than that in control group P< 0.05 and in the both of the hypertension group and the control group the lever of the plasma Hcy was different in sex.the males level was higer than the females P <0.05. The distribution of genotypic MS A

2 378 Journal Of Inner Mongolia Medical University Oct.2016 Vol.38 No.5 and allele frequencies had no significant difference between the two groups P > Conclusion There was probably no relation between MS A 2765 G genetic polymorphism and essential hypertension in Mongolians.Higher lever of the plasma Hcy may be the one of the risk factors in the Mongolian hypertensions. Key words homocysteine methionine synthase essential hypertension Mongolian 2002 DNA Bio Rad DNA 100ng /μl 18.1% MS A 2756 G PCR - 1~ RFLP 7 PCR homocysteinemia Hcy 20μL PCR 95 5min 94 30s 57 20s 72 45s min PCR 4 189bp 2% PCR Hcy PCR 10μL1μL 5 2μL ddh 2 O 20μL MS A 2756 G Hcy 37 24h PCR 96 PCR Hcy Monoblock 96 G SPSS 17.0 χ 2 t 165 x±s 珋 ~ ± ~ ± triacylglycerol TG BMI low density lipoprotein cholesterol LDL - C DBP LDL-C SBP fasting plasma glucose FPG FPG HDL-C high density lipoprotein cholesterol HDL-C uric cholesterol TC acid UA total Hcy EDTA-Na 3mL 60min <0.01 Hcy Hcy Hcy P<0.05 THERMO synergyht DNA EDTA -Na DNA LADER MAKERI MD101-1 HAE III PCR mix 1.3 Logistic P< Cr TC TG 2.2 Hcy Hcy P Hcy 2

3 MS A 2756 G MS A 2756 G Hcy Hardy-Weiberg P P> >0.05 MS A 2.5 Logistic 2756 G P> LDL-C DBP HDL-C BMI SBP Cr TC FPG TG 2.4 MS A 2756 G Hcy MS A 2756 G MS A 2756 G AA OR = %CI P = BMI OR 1 189bp AG 3 30bp 1 = %CI P = bp 189bp GG 2 30bp 159bp Tab.1 1 Comparison of the general characteristics % BIM SBP mmhg DBP mmhg ±9.86 * ** 27.96±5.10 * * 159.8±21.70 * * 96.44±10.76 * * ± ± ± ±9.27 * P<0.05 ** P<0.01 Tab.2 2 mmol /L Comparison of biochemical lindicators mmol /L FPG Cr TG TC HDL_C LDL_C ± ± ± ± ± ±0.64 ** ± ± ± ± ± ±0.37 ** P<0.01 Tab.3 3 Hcy μmol /L plasma Hcy in the two group μmol /L 15.76± ± ± ± ± ± P< Tab.4 4 MS A2756G n % The distribution of genotypes and allele frequencyes in MS A2756G n % AA AG GG AG+GG A G A = 2 AA + AG Hcy Hcy 8 11 Hcy Hcy 4 Hcy Hcy Hcy

4 380 Journal Of Inner Mongolia Medical University Oct.2016 Vol.38 No.5 Hcy MS 2756 GG AA AG Hcy Hcy MS A 2756 G 12 Hcy 13 BIM 1 M Marker AA 10 GG AG MS A 2756 G china a cross - sectional study J. PLoS One Fig.1 MS A 2756 G Gene polymorphism agarose gel electrophoregram 1 Wang H Zhang X Zhang J et al. Factors associated with prevalence Awareness Treatment and control of hypertension among adults in southern chian a community -based Cross - sectional survey J. PLoS One Zhou Y Zhou X Guo X et al. Prevalence and risk factors of hypertension among pre-and post-menopausal women a cross-sectional study in a rural area of northeast china J. Maturitas Gao Y Chen G Tian H et al.prevalence of hypertension in Veeranna V Zalawaadiya SK Niraj A et al. Homocysteine and reclassification off cardiovascular disease risk J.J Am Hcy Coll Cardiol Nabipour I Ebrahimi A Jafari SM et al. The metabolic syndrome is not association with homocysteinemia the persian gulf healthy heart study J. J Endocrinol Linvest Hcy Hcy 6. Hcy 2005 M Leclerc D Campeau E Goyette P et al.human methionine synthase cdna cloning and identifyion of mulations in patients of cb 1 G complementation group of folate /cobalam 14~ 17 Hcy in disorders J. Hum Mole Genet Hcy methionine synthase MS Hcy 8 Chobanian AV Bakris GL Black HR et al. Seventh report of the joint national committee on prevention Detection E- MS 2756A valuation And treatment of high blood pressure J.Hypertension G MS Morita 9 Rocha MS Teerlink T Janssen mch et al. Asymmentric G 15% dimethylarginine in adults with cystathionine B - synthase G deficiency J.Atherosclerosis ~ 19.52% 31 MS A G J

5 J J Vinukonda G Shaik mohammand N M d nurul jain J et al.genettic and environmental influences on total plasma homocysteine and coronary artery disease cad risk among south indians J. Clin Chim Acta / Giltay EJ Hoogeveen EK Elbers JM et al.effects of sexsteroids on plasma total homocysteine levels a study in transsexual males and females J. J Clin Endocrinol Metab Hill CH Mecham R.Starcher B fibrillin-2defects impairelastic fiber assembly in a homocysteinemic chick modal J.Joumal of Nutrition Mujumdar VS Aru GM Tyagi SC. Induction of oxidative stress by homocysteine impairs endothelial function J. Joumal of Cellular biochemistry mthfr A 1298 C 16. J J Tawakol A Omland T Gerhard M et al. Hyperhomocyst e memia is associated with impaired endothelium de- J pendent vasodilation in humans J.Circulation mthfr J J J. 19. msr J Morita H Kurihara H Sugiyama T et al.polymorphism of the methionine synthase gene associateon with homocysteine metabolism and late onset vascular disease in the japanese population J. Arteroscler Thromb Vasc Biol J Hcy cbst 833 C J Hcy mthfr C 677 T J J J J