Spectral Properties and Monosaccharides Compositon Analysis of Polysaccharide from Potentilla anserina L.

Transcription

1 Nat Prod Res Dev * jmyou6304@ 163. com * % α- NMP μg /mg μg /mg GC-MS R A Spectral Properties and Monosaccharides Compositon Analysis of Polysaccharide from Potentilla anserina L. XIA Lian SUN Zhi-wei 1 3 LI Guo-liang 1 3 SUO You-rui 1 YOU Jin-mao 1 2* 1 Key Laboratory of Life-Organic Analysis of Shandong Province College of Chemistry Science Qufu Normal University Qufu China 2 Northwest Plateau Institute of Biology the Chinese Academy of Sciences Xining China 3 Graduate University of Chinese Academy of Sciences Beijing China Abstract The polysaccharide of Potentilla anserine L. was extracted and purified and its contents was determined as 98. 4% by phenyl-sulfuric acid method. The polysaccharide was identified by IR spectrum and UV scanning spectrum. The IR spectrum indicates that the characteristic absorption peaks at cm -1 and 845 cm -1 belonged the characteristic peak of α-pyranose. In addition the peak at 1630 cm -1 assigned to the C = O of acetamide moiety stretching vibration meant that amino sugars exited in the polysaccharide. A method was developed for the separation of derivatized carbohydrates of Potentilla anserine L. using 1-naphthyl-3-methyl-5-pyrazolone NMP as derivatization reagent by capillary zone electrophoresis and the results shows that the monosaccharides compositions of the polysaccharide from Potentilla anserine L. are xylose arabinose glucose rhamnose mannose fucose galactose glucuronic acid and galacturonic acid with contents of μg / mg and μg /mg respectively which are consistent with the results carried out by GC-MS. Key words Potentilla anserine L. polysaccharide spectral properties monosaccharide composition Potentilla anserine L

2 454 Vol μg /ml 1 ml ~ 400 cm NMP μl M NMP 20 μl 15 sigma Milli-Q CARY 300 Varian Nicolet 10DX-FTIR HP6890 HP min 2 ml - HP-3D ph kv 10 Agilent s cm μm 10 mg mg 100 μl min 100 g100 ml 100 μl min h 100 ml 85% 30 min 800 μl 1 μl GC-MS 90 min 60 ml 3 95% 24 h % min 1. 2 ml /min 20 1 EI 90% 24 h mg 3 mol /L mg /ml savage mg ml ml 5% 1 ml 5 ml min μg /ml 10 min 488 nm A C 98. 4% mg /ml ~ 190 nm NMP 2 ml M 20 μl 17% cm 50 μm id 50 cm 55 mmol /L /min /min ev 55 ~ ml h N A C C = A r = ~ N 2

3 Vol nm Suzuki 260 nm 280 nm PMP C NMP 2 Fig. 1 1 The IR spectrum of polysaccharide from Potentilla anserine L ~ ~ ~ 1200 cm ~ 1000 cm -1 4 O-H C-H C-H C-O-H C-O-C C-O 1630 cm NMP cm -1 α- 890 Fig. 2 Derivatization scheme of 1-2-naphthyl -3-methyl-5- cm -1 pyrazolone NMP with reductive saccharides α β 2921 cm -1 NMP 250 ~ 5 μmol /L Table 1 1 n = 6 Linear regression equations correlation coefficients detection limits and repeatabilities for peak area and retention time of saccharide derivatives n = 6 Carbohydrates Regression equation * Relative RSD of RSD of migration time Detection limits cefficient migration time % % μmol /L S /N = 3 Xyl Y = X Ara Y = X Glc Y = X Rha Y = X Man Y = X Fuc Y = X Gal Y = X

4 456 Vol. 23 GlcUA Y = X GalUA Y = X * Y peak Area X concentration of monosaccharide μmol /L Fig. 3 3 Chromatograms of saccharides from Potentilla anserine L. sample by capillary zone electrophoresis GC-MS % 195 nm cm -1 α- Fig. 4 Quantitative of saccharides separated from Potentilla anserine L cm α μg / mg μg /mggc-ms 5 Fig. 5 Total ion chromatogram of acetylated derivatives of saccharides from Potentilla anserine L. sample GC-MS 1 Wu ZY. Xinhua Herbal Scheme 5. Shanghai Shanghai Science and Technology Press 1 Rha Fuc 3 Ara 4 Xyl 5 2 Qinghai institute of biology Tongren Man 6 Glc 7 Gal Longwu dispensary. Qinghai Altiplano Medicament Illustrated Handbook. 19 Xining Qinghai People s Press

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