Oxidative Stress: an Introduction
|
|
- Felix Webb
- 6 years ago
- Views:
Transcription
1 Oxidative Stress: an Introduction Helmut Sies Institute for Biochemistry and Molecular Biology I, Heinrich-Heine-University Düsseldorf, Germany
2 Oxidative Stress: Reactive Oxygen Species, Antioxidants Nutritional (dietary) Oxidative Stress: Postprandial Oxidative Stress Total Antioxidant Capacity (TAC) Plasma Redox Potentials, Glutathione Photooxidative Stress: Carotenoids, Flavonoids Optimum Dose of Antioxidants?
3 Michaelis, L.(1939) Cold Spring Harbor Symp., 33-49
4 Das Sauerstoff übertragende Ferment: Cytochrome-Oxidase Otto Warburg ( ) Kaiser-Wilhelm-Institut für Zellphysiologie, Berlin-Dahlem
5 Pathways of Hydrogen in the Living Organisation Angewandte Chemie 70 (1958)
6 In: Metabolic Compartmentation, Sies, H., ed. (1982) pp , Academic Press, London
7 Single cell image of rogfp1 targeted to HeLa cell mitochondria obtained by fluorescence microscopy The rogfp1 probe reports that the matrix space in HeLa cell mitochondria is highly reducing, with a midpoint potential near mv (assuming mitochondrial ph 8.0 at 37 C). Hanson GT et al (2004) J. Biol. Chem. 279,
8 Biological Half-Lives of Reactive Oxygen Species (ROS) (= Prooxidants) Sies, H. (1993) Eur. J. Biochem. 215:
9 Antioxidants in Biological Systems Enzymatic Nonenzymatic Superoxide Dismutases Ascorbate (Vitamin C) Glutathione Peroxidases Tocopherols (Vitamin E) Catalase Quinone Reductases Flavonoids Carotenoids Urate Glutathione (GSH)
10 Oxidative Stress Oxidative Stress Prooxidant - Antioxidant Balance Disturbance in favor of the prooxidants Pro- Oxidants Anti- Oxidants H. Sies (1985) in: Oxidative Stress, Academic Press, London, pp.1-8; (1986) Angewandte Chemie Int. Ed. 25,
11 Janus Oxidative Stress denotes a disturbance in the prooxidant/antioxidant balance in favor of the prooxidants, leading to potential damage In: Oxidative Stress (1985) Sies, H., Academic Press
12 Hydroperoxide Metabolism in Mammalian Systems Chance, Sies and Boveris (1979) Physiol. Revs. 58:
13 Oxidative Stress An imbalance between oxidants and antioxidants in favor of the oxidants, leading to a disruption of redox signaling and control and/or molecular damage. Sies, H, Jones, D.P. in: Encyclopedia of Stress (2007), Vol. 3, (Fink, G., ed.), Elsevier
14 Oxidative Stress: Reactive Oxygen Species, Antioxidants Nutritional (dietary) Oxidative Stress: Postprandial Oxidative Stress Total Antioxidant Capacity (TAC) Plasma Redox Potentials, Glutathione Photooxidative Stress: Carotenoids, Flavonoids Optimum Dose of Antioxidants?
15 Metabolic Fine-Tuning through Modulation by Dietary Micronutrients Postprandial Oxidative Stress Hyperglycemia Hypertriglyceridemia oxidizable Lipids oxidized Lipids Reactive Oxygen Species (ROS) ox- LDL Protection by Antioxidant Micronutrients e.g. Dietary Flavanols Endothelial Dysfunction Diabetic complications Atherosclerosis Sies, H., Stahl, W., Sevanian, A. (2005) J. Nutr. 135,
16 In industrialized societies, a significant part of the day is spent in the postprandial state Unsaturated fatty acids incorporated into LDL are suitable targets for oxidation, and oxidized LDL are an atherogenic factor. Lipid hydroperoxides already present in the diet are absorbed and contribute to the prooxidant load.
17 Effect of a Single High-Fat meal on Endothelial Function in Healthy Subjects Vogel RA, Corretti MS, Plotnick GD (1997) Am J Cardiol 79,
18
19 Vasoprotective endothelial effects of a standardized grape product in humans High-Fat Meal + Grape Product (standardized) High-Fat meal Chaves AA et al (2009) Vasc Pharmacol 50, 20-26
20 Red wine mitigates the postprandial increase of LDL susceptibility to oxidation. Natella et al (2001) Free Radic Biol Med. 30:
21 Postprandial oxidative stress is attenuated when dietary antioxidants are supplied together with a meal rich in oxidized or oxidizable lipids. Ingestion of dietary polyphenols, e.g. from wine, cocoa, or tea, improves endothelial dysfunction and lowers the susceptibility of LDL lipids to oxidation.
22 High-flavanol cocoa drink ameliorates endothelial function in vivo through improved bioactivity and bioavailability of NO Flow-mediated dilation (%) * RNO in plasma (nmol/l) * 0 before 2 h after before 2 h after low-flavanol cocoa drink high-flavanol cocoa (17 mg/100ml) drink (176mg/10 0ml) 0 before 2 h after before 2 h after low-flavanol high-flavanol cocoa drink cocoa drink C. Heiss et al (2003) JAMA 290,
23 Oxidative Stress: Reactive Oxygen Species, Antioxidants Nutritional (dietary) Oxidative Stress: Postprandial Oxidative Stress Total Antioxidant Capacity (TAC) Plasma Redox Potentials, Glutathione Photooxidative Stress: Carotenoids, Flavonoids Optimum Dose of Antioxidants?
24 The issue of TAC Is there a use in non-compositional assays of antioxidant capacity?
25 A pioneering paper... Quantitative measurement of the total, peroxyl radical-trapping antioxidant capability of human blood plasma by controlled peroxidation. The important contribution made by plasma proteins. Keith Ingold Wayner, Burton, Ingold, Locke (1985) FEBS Lett 187:33-7
26 followed by a flurry of assay methods... TRAP, TEAC, ORAC, FRAP...: Total Radical-trapping Antioxidant Parameter (TRAP) Trolox Equivalent Antioxidant Capacity (TEAC) Oxygen Radical Absorbance Capacity (ORAC) Ferric Reducing Antioxidant Power (FRAP)... Generic: TAC, Total Antioxidant Capacity
27 Transfer to the Public...
28 Note: TAC applied to in vivo: neither Total nor the real Antioxidant Capacity is measured what is measured by TAC is mainly ascorbate, urate and other minor components; in vivo antioxidant enzyme support is not assessed in isolated fluids out of steady-state
29 % Erhola et al Wayner et al. Tubaro et al Benzie & Strain Courtesyof G. Bartosz, Lodz
30 Upshot: TAC in vitro (e.g. in foods or extracts): OK TAC in vivo (e.g. in plasma): not OK Publication of such data is encouraged Publication of such data is discouraged See: Total antioxidant capacity: appraisal of a concept. Sies, H. (2007) J Nutr. 137:1493-5
31 Sies, H. (2007) J Nutr 137,
32 Oxidative Stress: Reactive Oxygen Species, Antioxidants Nutritional (dietary) Oxidative Stress: Postprandial Oxidative Stress Total Antioxidant Capacity (TAC) Plasma Redox Potentials, Glutathione Photooxidative Stress: Carotenoids, Flavonoids Optimum Dose of Antioxidants?
33 Glutathione (L-γ-glutamyl-L-cysteinyl-glycine) GSH Transferases GSH Peroxidases GSSG Reductase γ Glutamyl Transpeptidase Sies, H. (1999) FRBM 27,
34 GLUTATHIONE What does it do? Roles in: signal transduction gene expression; protein folding cell proliferation apoptosis
35 Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple Oxidation-Reduction Potential (E(h)) of GSSG/2GSH correlates with the biological status of the cell: Proliferation E(h) approx mv Differentiation E(h) approx mv Apoptosis E(h) approx mv Schafer F, Buettner G (2001) Free Rad Biol Med 30,
36 Meier et al (1989) Biochem. J. 263,
37 Cytokines also regulate production of NO via intracellular signal pathways after Kroencke (2002)
38 Oxidative Stress: Reactive Oxygen Species, Antioxidants Nutritional (dietary) Oxidative Stress: Postprandial Oxidative Stress Total Antioxidant Capacity (TAC) Plasma Redox Potentials, Glutathione Photooxidative Stress: Carotenoids, Flavonoids Optimum Dose of Antioxidants?
39 Light Electronic Excitation Sensitizer Photooxidative Stress Sensitizer* ROS Singlet Oxygen LIPIDS DNA PROTEIN
40 Sens hν 1 Sens* ISC Car 3 Sens* Energytransfer 3 3 O2 Car* Reaction rate constant: Quenching of 1 O (M -1 s -1 ) ß-Carotene 8 Lutein 8 Zeaxanthin 10 Lycopene 15 4-OH-ß-Carotene 9 Cryptoxanthin 9 Capsorubin 8 Sens + 1 O 2 Car
41 Carotenoids and Oxocarotenoids Polyene Structure The essential function of carotenoids is to prevent harmful photooxidative reactions There would be no photosynthesis were it not for the presence of carotenoids : Plants would get sunburnt!
42 Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Second-order rate constant near diffusion limit Di Mascio et al (1989) Arch. Biochem. Biophys. 274,
43 Irradiated Skin: Reddening 24 h after UV-Exposition a value
44 Lycopene protects against UV-induced erythema Serum Lycopene Skin Carotenoids a Value Serum Lycopene (µm) 1,00 0,75 0,50 0,25 * * Skin Carotenoids inside arm (nmol/g) 0,6 0,4 0,2 Erythema - -a value ns * day 0 wk 4 wk 10 day 0 wk 4 wk 10 day 0 wk 4 wk 10 n = 10; p = Stahl et al (2001) J. Nutr. 131:
45 Dietary tomato paste protects against ultraviolet light-induced erythema in humans Stahl et al (2001) J Nutr 131:
46 Cocoa Polyphenols: Flavanols Cocoa with high flavanol content protects against UV-induced erythema 6 Low flavanol High flavanol -a-value * * week *significantly different from wk 0; p < 0.05 Heinrich et al (2006) J. Nutr. 136,
47 High-frequency ultrasound scan skin density at week 0 after 12 weeks of consuming high flavanol cocoa beverage Heinrich et al (2006) J. Nutr. 136,
48 Skin surface profiles at week 0 after 12 weeks of consuming high flavanol cocoa beverage Corresponding top view of skin at week 0 after 12 weeks Heinrich et al (2006) J. Nutr. 136,
49 Oxidative Stress: Reactive Oxygen Species, Antioxidants Nutritional (dietary) Oxidative Stress: Postprandial Oxidative Stress Total Antioxidant Capacity (TAC) Plasma Redox Potentials, Glutathione Photooxidative Stress: Carotenoids, Flavonoids Optimum Dose of Antioxidants?
50 Is there an optimum dose?
51 UV-Induced Lipid Peroxidation in Skin Fibroblasts 2,00 1,80 Lycopene prooxidant TBARS/Protein [pmol/µg] 1,60 1,40 1,20 1,00 0,80 0,60 0,40 antioxidant Control: lycopene -free cells exposed to UV-light; TBARS 0.98 ± 0.11 pmol MDA/µg protein 0,20 0,00 0,00 0,10 0,20 0,30 0,40 0,50 Lycopene/Protein [pmol/µg] Eichler et al (2002) Photochem. Photobiol. 75,
52 Odds ratios for peripheral arterial disease by serum selenium levels Curves represent adjusted odds ratios (solid line) Bleys J et al (2009) Amer J Epidemiol 169:
53 Acknowledgements Wilhelm Stahl Tankred Schewe Cristina Polidori Lars-Oliver Klotz Institut für Umweltmedizinische Forschung, Heinrich-Heine-Universität Düsseldorf Deutsche Forschungsgemeinschaft Ernst-Jung-Stiftung für Medizin National Foundation for Cancer Research (NFCR), Bethesda
Reactive Oxygen species ROS + Anti-oxidants. Dr. Naif Karadsheh
Reactive Oxygen species ROS + Anti-oxidants Dr. Naif Karadsheh Oxygen Toxicity & Free Radicals Biradical O 2 Radical O 2 Non-Radical Radical H 2 O 2 OH ROS O 2 Metabolism and Toxicity O 2 Consumption >90%
More informationMid Term Test- Template Questions
Mid Term Test- Template Questions Name : ID : Time: Date: Total points: 100 Instructions: 1. Only 20 questions from Section I and II, 10 questions from Section III will be counted for grading. 2. Try to
More information6. SUMMARY AND CONCLUSION
6. SUMMARY AND CONCLUSION Free radicals are chemical species containing one or more unpaired electrons, like hydrogen atom, most transition metal ions, nitric oxide and oxygen, with two unpaired electrons.
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationLipid Oxidation and its Implications to Food Quality and Human Health. Dong Uk Ahn Animal Science Department Iowa State University
Lipid Oxidation and its Implications to Food Quality and Human Health Dong Uk Ahn Animal Science Department Iowa State University Introduction Process of Lipid Oxidation Free Radicals and Reactive Oxygen
More informationORAC Assay Kit KF A/ B 96/ 192 tests (96 well plate)
ORAC Assay Kit KF-01-004 A/ B 96/ 192 tests (96 well plate) Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Assay protocol Pag. 4 Data analysis Pag. 8 References Pag. 9 Warranties and
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2001 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2001) offered
More informationBiologic Oxidation BIOMEDICAL IMPORTAN
Biologic Oxidation BIOMEDICAL IMPORTAN Chemically, oxidation is defined as the removal of electrons and reduction as the gain of electrons. Thus, oxidation is always accompanied by reduction of an electron
More informationBiological Chemistry of Hydrogen Peroxide
Biological Chemistry of Hydrogen Peroxide Christine Winterbourn Department of Pathology University of Otago, Christchurch New Zealand Hydrogen Peroxide Intermediate in reduction of oxygen to water A major
More informationWhat is an antioxidant? How do antioxidants work?
What is an antioxidant? How do antioxidants work? Garry R. Buettner and Freya Q. Schafer Free Radical and Radiation Biology Program and ESR Facility The University of Iowa Iowa City, IA 52242-1101 Tel:
More informationLujain Hamdan. Faisal Nimri
20 Lujain Hamdan Faisal Nimri...... Sources of NADPH [ The pentose phosphate pathway is the primary source of the NADPH and is the only source in RBC.] Cytosolic conversion of oxaloacetate to pyruvate
More informationRelationship between serum glutathione peroxidase-1activity with endothelial dysfunction level in patients with coronary artery diseases
Relationship between serum glutathione peroxidase-1activity with endothelial dysfunction level in patients with coronary artery diseases Introduction Reactive oxygen species (ROS),such as superoxide and
More informationAntioxidant Products
Antioxidant Products Introduction Introduction Antioxidant Total Antioxidant Status (TAS) Ransel Ransod Glutathione Reductase Antioxidants help defend living organisms against free radical attack. Many
More informationThe levels of oxidative stress and antioxidants in diabetes mellitus before and after diabetic treatment with or without antioxidants
Original article: The levels of oxidative stress and antioxidants in diabetes mellitus before and after diabetic treatment with or without antioxidants *Sarita A Shinde, Anita D. Deshmukh, Adinath N. Suryakar,
More informationnumber Done by Corrected by Doctor
number 19 Done by حسام ابو عوض Corrected by وسيم ابو عبيدة Doctor د.نايف 1 P a g e GAGs and Glycoproteins: GAGs: long, unbranched heteropolysaccharides, made from زunits repeating disaccharide [Acidic
More informationNumerous epidemiology studies support the concept that diets rich in plant foods are associated with a reduced risk for vascular disease.
13 Numerous epidemiology studies support the concept that diets rich in plant foods are associated with a reduced risk for vascular disease. Fruits/Vegetables - Multivariate adjusted RR for >=3 times/day
More informationMyBioSource.com. OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit. Product Manual. Catalog Number
Product Manual OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit Catalog Number Introduction Oxidative stress is a physiological condition where there is an imbalance between concentrations
More informationDMPD Assay Kit KF tests (96 well plate)
DMPD Assay Kit KF-01-001 200 tests (96 well plate) Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Sample preparation Pag. 4 Assay protocol Pag. 5 Data analysis Pag. 9 References Pag.
More informationTheory Photochem. Anna Horszwald (Michalska)
Theory Photochem Anna Horszwald (Michalska) Free radicals Free radicals inflamation mitochondrial disfuntion Lachance P. A. et al. (2001) Antioxidants an intergarative approach, Nutrition, 17,835-838.
More information-Carotene affects antioxidant status in non-insulin-dependent diabetes mellitus
Pathophysiology 6 (1999) 157 161 www.elsevier.com/locate/pathophys -Carotene affects antioxidant status in non-insulin-dependent diabetes mellitus Yishai Levy *, Haya Zaltzberg, Ami Ben-Amotz, Yoram Kanter,
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2003 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2003) offered
More informationLecture 19 Summary Gestational Diabetes and Complications of Diabetes. Gestational diabetes;
Lecture 19 Summary Gestational Diabetes and Complications of Diabetes Gestational diabetes; - Type of diabetes that only develops during pregnancy Usually diagnosed in late pregnancy Causes high blood
More informationQuality Assurance of Nutraceutical Health Claims: The Case for Antioxidants
Quality Assurance of Nutraceutical Health Claims: The Case for Antioxidants Jan Knight and Robert Knight Knight Scientific Limited The consumer of nutraceuticals perceives natural products to be safer
More informationEFFECT OF LYCOPENE IN TOMATO SOUP AND TOMATO JUICE ON THE LIPID PROFILE OF HYPERLIPIDEMIC SUBJECTS
EFFECT OF LYCOPENE IN TOMATO SOUP AND TOMATO JUICE ON THE LIPID PROFILE OF HYPERLIPIDEMIC SUBJECTS Nora Vigasini, Assistant Professor, Department of Home Science, Women s Christian College, Chennai, noravigas@gmail.com
More informationNAB Rhodiola Extract Your Partner in the Fight Against Urban Stress
Personal Care NAB Rhodiola Extract Your Partner in the Fight Against Urban Stress Key Product Attributes Adaptogenic plant extract that helps protect skin against the stresses of modern urban life Enhances
More informationEVALUATION OF ANTIOXIDANT ACTIVITY OF NEW NUTRACEUTICALS: IN VITRO AND CELLULAR ASSAYS. Ana Denicola
EVALUATION OF ANTIOXIDANT ACTIVITY OF NEW NUTRACEUTICALS: IN VITRO AND CELLULAR ASSAYS Ana Denicola Lab. Fisicoquímica Biológica. Instituto Química Biológica. Facultad de Ciencias, UdelaR. Center for Biomedical
More informationRenoprotective effects of carotenoid on the Kidneys of Adult Wistar Rats
International Journal of Scientific and Research Publications, Volume 4, Issue 2, February 2014 1 Renoprotective effects of carotenoid on the Kidneys of Adult Wistar Rats Ezejindu D. N., Nwokoro Tochukwu,
More informationProtective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin
American Journal of Clinical and Experimental Medicine 2017; 5(2): 41-45 http://www.sciencepublishinggroup.com/j/ajcem doi: 10.11648/j.ajcem.20170502.13 ISSN: 2330-8125 (Print); ISSN: 2330-8133 (Online)
More informationABTS Assay Kit KF tests (96 well plate)
ABTS Assay Kit KF-01-002 250 tests (96 well plate) Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Sample preparation Pag. 4 Assay protocol Pag. 5 Data analysis Pag. 9 References Pag.
More informationNutritional facts of fruit and health benefits
Training 2 - Fruit consumption Ohrid, 23 June 2009 Nutritional facts of fruit and health benefits Julie Mardon Enita Clermont, France Introduction Taste Texture Color Fruit Safety (pesticides, microbiological
More informationNUTRITIONAL PROTECTION AGAINST SKIN DAMAGE FROM SUNLIGHT
Annu. Rev. Nutr. 2004. 24:173 200 doi: 10.1146/annurev.nutr.24.012003.132320 Copyright c 2004 by Annual Reviews. All rights reserved First published online as a Review in Advance on February 6, 2004 NUTRITIONAL
More informationCornstarch
Electronic Supplementary Material (ESI) for Food & Function. This journal is The Royal Society of Chemistry 2018 Supplementary data : Supplementary Table 1: Diet composition (g/kg) on the basis of the
More information1 INTRODUCTION. Page 1
INTRODUCTION 1 INTRODUCTION You are what you eat. Diet is an integral part of human life which indirectly reflects the physical and emotional state of a person. There is a huge diversity in food patterns
More informationTHE ANTIOXIDANT REVOLUTION - TO PROTECT AGAINST DISEASES & TO MAINTAIN OPTIMUM HEALTH AND WELLBEING
ISSN 2249-7641 Print ISSN 2249-765X International Journal of Pharmacology Research www.ijprjournal.org THE ANTIOXIDANT REVOLUTION - TO PROTECT AGAINST DISEASES & TO MAINTAIN OPTIMUM HEALTH AND WELLBEING
More informationLipid Oxidation in Muscle Foods
Lipid Oxidation in Muscle Foods Unique Challenges with Oxidation in Muscle Foods Rancidity is a major shelf-life limiting factor in frozen muscle foods NaCl generally accelerates oxidation Oxidation accelerates
More information02/12/13. Marketing Local Tomatoes by Promising Health Benefits. Consumer Health and Eating Habits. Alan Walters Ruplal Choudhary Travis Killion
Marketing Local Tomatoes by Promising Health Benefits Alan Walters Ruplal Choudhary Travis Killion Southern Illinois University Consumers today base part of their fresh produce purchase decisions on eating
More informationMechanistic Toxicology
SECOND EDITION Mechanistic Toxicology The Molecular Basis of How Chemicals Disrupt Biological Targets URS A. BOELSTERLI CRC Press Tavlor & France Croup CRC Press is an imp^t o* :H Taylor H Francn C'r,,jpi
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2003 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2003) offered
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationTechnical Overview of Micronutrient Testing
Technical Overview of Micronutrient Testing J.F. Crawford, Ph. D. SpectraCell Laboratories, Inc. Houston, TX Recent Publications Antioxidant supplementation increases morbidity and mortality. JAMA, 2007
More informationClean Label Solutions to Controlling Lipid Oxidation
Clean Label Solutions to Controlling Lipid Oxidation Eric A Decker Department of Food Science University of Massachusetts, Amherst, USA March 27-28, 2018 Itasca, Illinois, USA Why is Oxidation Important?
More informationAntioxidants in food, drinks and supplements for cardiovascular health
Position statement Antioxidants in food, drinks and supplements for cardiovascular health This position statement provides recommendations for the consumption of antioxidantrich food, drinks and supplements
More informationFree Radicals in Biology and Medicine
Free Radicals in Biology and Medicine 0 \ Second Edition BARRY HALLIWELL Professor of Medical Biochemistry, University of London King's College and JOHN M.C. GUTTERIDGE Senior Scientist, National Institute
More informationBiochemistry: A Short Course
Tymoczko Berg Stryer Biochemistry: A Short Course Second Edition CHAPTER 20 The Electron-Transport Chain 2013 W. H. Freeman and Company Chapter 20 Outline Oxidative phosphorylation captures the energy
More informationOxidation general concepts. Prof. Umberto Cornelli Loyola University Medical School-Chicago
Oxidation general concepts Prof. Umberto Cornelli Loyola University Medical School-Chicago 1 Antioxidants a real mess! Is oxidative stress a real problem? Is every antioxidant good for health? Oxidation
More informationBiomarkers of Oxidative Stress and Antioxidant Status in Type 2 Diabetes: Importance in treatment Professor Grace George
Division of Medical Biochemistry, Department of Human Biology, Faculty of Health Sciences, Mthatha, South Africa Biomarkers of Oxidative Stress and Antioxidant Status in Type 2 Diabetes: Importance in
More informationOSAS oxidative stress and atherosclerosis Who is the culprit: AHI or ODI
OSAS oxidative stress and atherosclerosis Who is the culprit: AHI or ODI Arie Wollner MD, FCCP Department of Respiratory Care and Rehabilitation Sheba Medical Center OSA as a major public health problem
More informationTHE TOTAL ANTIOXIDANT CAPACITY OF BLOOD PLASMA DURING CARDIOVASCULARY BYPASS SURGERY IN PATIENTS WITH CORONARY HEART DISEASE
CELLULAR & MOLECULAR BIOLOGY LETTERS Volume 8, (2003) pp 973 977 http://www.cmbl.org.pl Received 1 September 2003 Accepted 28 October 2003 Short Communication THE TOTAL ANTIOXIDANT CAPACITY OF BLOOD PLASMA
More informationEM-X Herbal Tea Inhibits Interleukin-8 Release in Alvelor Epithelial cells
EM-X Herbal Tea Inhibits Interleukin-8 Release in Alvelor Epithelial cells Okezie I. Arouma a) and Irfan Rahman b) a) Department of Neuroinflammation, Division of Neuroscience & Psychological Medicine,
More informationORAC Values. Antioxidant measurement units
ORAC Values ORAC (Oxygen Radical Absorption Capacity) is a standardised test that was adopted by the U.S. Department of Agriculture to measure the Total Antioxidant Potency of foods and nutritional supplements.
More informationFunctional Properties of Cocoa Products: Health Aspects. Puspo Edi Giriwono SEAFAST Center - IPB
Functional Properties of Cocoa Products: Health Aspects Puspo Edi Giriwono SEAFAST Center - IPB Theobroma cacao Nutrient content of various cocoa products Colombo et al., Chocolate and Health., 2012 Cocoa
More informationnumber Done by Corrected by Doctor Nayef Karadsheh
number 17 Done by Abdulrahman Alhanbali Corrected by Lara Abdallat Doctor Nayef Karadsheh 1 P a g e Pentose Phosphate Pathway (PPP) Or Hexose Monophosphate Shunt In this lecture We will talk about the
More informationPII S (00) CONSUMPTION OF TOMATO PRODUCTS WITH OLIVE OIL BUT NOT SUNFLOWER OIL INCREASES THE ANTIOXIDANT ACTIVITY OF PLASMA
PII S0891-5849(00)00440-8 Free Radical Biology & Medicine, Vol. 29, No. 10, pp. 1051 1055, 2000 Copyright 2000 Elsevier Science Inc. Printed in the USA. All rights reserved 0891-5849/00/$ see front matter
More informationA deficiency of biotin, commonly seen in alcoholics, can cause neurological symptoms
Water-soluble vitamins Vitamin deficiencies Metabolism General Diseases etc. A deficiency of biotin, commonly seen in alcoholics, can cause neurological symptoms Levels of folate are particularly low in
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered
More informationAging and nutrition 03/11/2012. Why do people age? Oxidative stress and damage
Aging and nutrition % of elderly people in Canadian population is increasing more than for other age groups within the elderly age group there is great variability in terms health, metabolism, physical
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2003 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2003) offered
More informationSource Variation in Antioxidant Capacity of Cranberries from Eight U.S. Cultivars
33 Source Variation in Antioxidant Capacity of Cranberries from Eight U.S. Cultivars Peter J. Schaaf Faculty Sponsors: Margaret A. Maher and Ted Wilson, Departments of Biology/Microbiology ABSTRACT Antioxidants
More informationThis student paper was written as an assignment in the graduate course
77:222 Spring 2003 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2003) offered
More informationAN ACTIVE SHELTER AGAINST POLLUTION V.16
AN ACTIVE SHELTER AGAINST POLLUTION V.16 THE SKIN IS CONSTANTLY EXPOSED The skin is an interface and thus is constantly exposed to environmental agents: Sun radiation Smoke Air pollution Free radicals
More informationCardiovascular impact of Sugar and Fat
Professor Göran Petersson May 2011 Chemical and biological engineering Chalmers University of Technology Göteborg, Sweden Less sugar and soft drinks Less food with high GI Less omega-6 fatty acids More
More informationMediterranean Diet: Choose this heart-healthy diet option
Mediterranean Diet: Choose this heart-healthy diet option The Mediterranean diet is a heart-healthy eating plan combining elements of Mediterraneanstyle cooking. Here s how to adopt the Mediterranean diet.
More informationLifePak Health Benefits. LifePak is optimum supplementation for:
LifePak What is LifePak? Our comprehensive nutritional wellness program delivering the optimum amounts of all essential and generally beneficial nutrients for long-term health and well-being. LifePak Health
More informationGlutathione / Thioredoxin Nrf2 & Hyperoxia
Glutathione / Thioredoxin Nrf2 & Hyperoxia Trent E. Tipple, MD Principal Investigator, Center for Perinatal Research The Research Institute at Nationwide Children s Hospital Assistant Professor of Pediatrics,
More informationAntioxidant Measurement and Applications: An Overview
Chapter 1 Antioxidant Measurement and Applications: An Overview Downloaded via 148.251.232.83 on December 9, 2018 at 23:57:09 (UTC). See https://pubs.acs.org/sharingguidelines for options on how to legitimately
More informationPeter Surai, PhD, DSc Feed-Food. Ltd., Scotland, UK Szent Istvan University, Godollo, Hungary Trakia University, Stara Zagora, Bulgaria
Nutritional modulation of the antioxidant system of the body Peter Surai, PhD, DSc Feed-Food. Ltd., Scotland, UK Szent Istvan University, Godollo, Hungary Trakia University, Stara Zagora, Bulgaria Free
More informationROLE OF MINERAL NUTRITION IN ALLEVIATING DETRIMENTAL EFFECTS OF ENVIRONMENTAL STRESSES ON CROP PRODUCTION
ROLE OF MINERAL NUTRITION IN ALLEVIATING DETRIMENTAL EFFECTS OF ENVIRONMENTAL STRESSES ON CROP PRODUCTION by Ismail CAKMAK Sabanci University Istanbul, Turkiye HUGE INCREASES IN WORLD POPULATION FOOD SECURITY
More informationINTRODUCTION
CHAPTER I 37 3.1.0.0. INTRODUCTION Molecular oxygen is an essential nutrient for higher forms of life. In addition to its normal physiological reactions, oxygen and hs partially reduced forms, ROS oxidize
More informationBIOASTIN Bioastin is product that contains 4 mg astaxantin and 50 i.e. vitamin E (α-tocopherol) per tabl.. Carotenoids are family of around 700 substa
INTRODUCTION In 1956 Rebecca Gerchman and Daniel Gilbert detected toxic effect of superoxid on aerobs. Till today there are identificated many free radicals. Their toxic effect is registrated in more than
More informationFood, Supplements and Healthy Lifestyles in Slowing and Preventing Macular Degeneration Julie Mares, Professor For the Nutrition and Eye Health Research Team http://nutritionforeyes.ophth.wisc.edu Preserving
More information- Spontaneous hemorrhaging increase
MEDCHEM 562-2014 Fat Soluble Vitamins Problem Set 1. Fill out the blanks in the table below. Vitamin Physiological Function Deficiency symptoms Toxicity A -Vision -Cell Differentiation -Inhibition of Cell
More informationTHERMALLY PROCESSED FOODS: Possible health implications
COST-927 Action THERMALLY PROCESSED FOODS: Possible health implications Building Skills on the Determination of the Overall Antioxidant Capacity of Thermally Processed Foods Olsztyn, 26-30 January 2009
More informationMembrane lipid peroxidation by ultrasound: Mechanism and implications
J. Biosci., Vol. 15, Number 3, September 1990, pp. 211 215. Printed in India. Membrane lipid peroxidation by ultrasound: Mechanism and implications A. K. JANA, S. AGARWAL and S. N. CHATTERJEE* Biophysics
More informationThe LiVicordia study
CARDIOVASCULAR HEALTH AND LIFESTYLE: NORDIC-BALTIC COMPARISON The LiVicordia study Zita Kučinskienė On behalf of the LiVicordia group Mortality among men / 100000 from Ischemic heart diseases (410-414).
More informationThe Effect of High Dose IV Vitamin C on Plasma Antioxidant Capacity and Level of Oxidative Stress in Cancer Patients and Healthy Subjects
The Effect of High Dose IV Vitamin C on Plasma Antioxidant Capacity and Level of Oxidative Stress in Cancer Patients and Healthy Subjects N.A Mikirova, Ph.D.; J.A. Jackson, Ph.D., MT(ASCP); Neil H Riordan,
More informationWhat s the Score? What is ORAC?
What s the Score? Neways recently sent samples of Revenol and Cascading Revenol to be tested to find out what their ORAC scores are. Read on to discover their ratings and what their ORAC scores mean for
More informationOxidative Stress, Oxidative Damage and Antioxidants A Beginners Guide
Oxidative Stress, Oxidative Damage and Antioxidants A Beginners Guide David J Marlin 1 and Catherine E Dunnett 2 David Marlin Consulting Ltd, Newmarket, Suffolk, UK 1 Independent Equine Nutrition Ltd,
More informationGemma Cooper University of Nottingham
Gemma Cooper University of Nottingham mbxgc@nottingham.ac.uk Outline Introduction to flavanols What are flavanols? What do flavanols do? Flavanols In the diet In apples and apple juice My work Current
More informationOxiSelect Hydrogen Peroxide Assay Kit (Colorimetric)
Product Manual OxiSelect Hydrogen Peroxide Assay Kit (Colorimetric) Catalog Number STA-343 5 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Oxidative stress is a physiological
More informationSynergistic effects of antioxidative peptides from rice bran
Synergistic effects of antioxidative peptides from rice bran Pichamon Kiatwuthinon 1,*, Neeracha Lapanusorn 1, Anunyaporn Phungsom 1, Wirawan Tinanchai 1 1 Department of Biochemistry, Faculty of Science,
More informationIvan Petyaev. Cambridge UK 16TH FOOD INNOVATE SUMMIT AMSTERDAM APRIL 2018
Ivan Petyaev Cambridge UK 16TH FOOD INNOVATE SUMMIT AMSTERDAM APRIL 2018 What we do Develop technologies and product prototypes for Functional Food and Beverages Laboratory and Clinical Validation License
More informationMetabolism of xenobiotics FM CHE 5-6
Metabolism of xenobiotics FM 3.5-10 CHE 5-6 Metabolism of xenobiotics Also called:! Biotransformation, or detoxification Primary metabolism secondary metabolism Goal: To make use of a compound or to facilitate
More informationNM SACHINDRA JSPS Post-Doctoral Research Fellow. Prof. K MIYASHITA. Faculty of Fisheries Hokkaido University, Hakodate
NM SACHINDRA JSPS Post-Doctoral Research Fellow Prof. K MIYASHITA Faculty of Fisheries Hokkaido University, Hakodate Mysore India Karnataka Working as: Scientist Central Food Technological Research Institute
More informationCopyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and
Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere
More informationI Antonio Cilla Tatay PhD Postdoctoral researcher
ANTICARCINOGENIC EFFECT OF PHYTOSTEROLS AND/OR BETA-CRYPTOXANTHIN IN COLON CANCER CELLS I Antonio Cilla Tatay PhD Postdoctoral researcher E-mail: antonio.cilla@uv.es Nutrition and Food Science Area. Faculty
More informationSFRBM Annual Meeting, Pre-meeting Workshop II Flavanols in Health and Disease
FLAVIOLA Targeted delivery of dietary flavanols for optimal human cell function: Effects on cardiovascular health SFRBM Annual Meeting, Pre-meeting Workshop II Flavanols in Health and Disease Flavanol
More informationIntolerance in Heart Failure
Novel Targets to Attack Exercise Intolerance in Heart Failure - Skeletal Muscle - Volker Adams, PhD ESC, Paris 3. Aug. 211 UNIVERSITÄT LEIPZIG H E R Z Z E N T R U M Nothing to disclose Myers et al. NEJM
More informationASTAXANTHOL - #1057. Anti-Inflammatory, Anti-Aging Support for the Whole Body
ASTAXANTHOL - #1057 Anti-Inflammatory, Anti-Aging Support for the Whole Body Astaxanthol is a uniquely designed, nutraceutical formulation that is an extremely potent, highly bi-available, broad spectrum
More informationAntioxidants: Taking a Closer Look at Vitamin C and E. Kerry B. Barbera. University of Alabama.
Antioxidants: Taking a Closer Look at Vitamin C and E Kerry B. Barbera University of Alabama kbbarbera@crimson.ua.edu Introduction Oxygen sustains life but it is also responsible for creating oxidative
More informationNATURES BEST GREENS SIMPLY SHAKE WELL (THE SEEDS MAY HAVE SETTLED) TEAR THE TOP OFF SQUEEZE AND EXPERIENCE PURE SEED NUTRITION
NATURES BEST GREENS SIMPLY SHAKE WELL (THE SEEDS MAY HAVE SETTLED) TEAR THE TOP OFF SQUEEZE AND EXPERIENCE PURE SEED NUTRITION SEEDS. It s a simple concept, and you re going to love it. We ve taken the
More informationLOOKING FOR LIPID PEROXIDATION IN VITRO AND IN VIVO: IS SEEING BELIEVING? Vanderbilt University School of Medicine Jason D.
LOOKING FOR LIPID PEROXIDATION IN VITRO AND IN VIVO: IS SEEING BELIEVING? Vanderbilt University School of Medicine Jason D. Morrow MD Which of the following assays of lipid peroxidation may be useful and
More information~PENTOSE PHOSPHATE PATHWAY~ DR. A. TARAB DEPT. OF BIOCHEMISTRY HKMU
~PENTOSE PHOSPHATE PATHWAY~ DR. A. TARAB DEPT. OF BIOCHEMISTRY HKMU OVERVIEW The pentose phosphate pathway (also called the hexose monophosphate shunt, or 6- phosphogluconate pathway) occurs in the cytosol
More informationshehab Moh Tarek ... ManarHajeer
3 shehab Moh Tarek... ManarHajeer In the previous lecture we discussed the accumulation of oxygen- derived free radicals as a mechanism of cell injury, we covered their production and their pathologic
More informationMouse Hydrogen Peroxide (H2O2) Fluorescent Detection Kit
Mouse Hydrogen Peroxide (H2O2) Fluorescent Detection Kit CATALOG NO: IRAAKT2552 LOT NO: SAMPLE INTENDED USE The Hydrogen Peroxide Fluorescent Detection Kit is designed to quantitatively measure H2O2 in
More information10 MISCONCEPTIONS ABOUT ANTIOXIDANTS
10 MISCONCEPTIONS ABOUT ANTIOXIDANTS Excitement around antioxidants has diminished, replaced by polarised views and misconceptions that have started to impede industry use of the ingredient in supplements
More informationSensitivity of mammalian cells to higher concentrations of reactive oxygen species induced by radiation or chemical treatment
Sensitivity of mammalian cells to higher concentrations of reactive oxygen species induced by radiation or chemical treatment The reactive oxygen species (ROS) are group of very unstable compounds that
More informationFactors Affecting Oxidative Stability of Pork, Beef, and Chicken Meat
Animal Industry Report AS 654 ASL R2257 2008 Factors Affecting Oxidative Stability of Pork, Beef, and Chicken Meat Byung R. Min Ki C. Nam Joseph C. Cordray Dong U. Ahn, duahn@iastate.edu Recommended Citation
More informationTargeted Health Regimen Every Targeted Health Regimen builds upon the Foundations of Optimal Health Regimen. Vision Health Regimen
Targeted Health Regimen Every Targeted Health Regimen builds upon the Foundations of Optimal Health Regimen. Vision Health Regimen Declining vision is a result of the normal aging process. Why should we
More informationBIOCHEMICHISTRY OF EYE TISSUE. Sri Widia A Jusman Department of Biochemistry & Molecular Biology FMUI
BIOCHEMICHISTRY OF EYE TISSUE Sri Widia A Jusman Department of Biochemistry & Molecular Biology FMUI 1 METABOLIC PATHWAYS IN EYE TISSUE Glycolysis ( aerobic & anaerobic) HMP shunt Poliol pathway TCA cycle
More information