Protease and Phosphatase Inhibitor Cocktail, Brochure

Transcription

1 Protease and Phosphatase Inhibitor Cocktail, Brochure Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0) T BE +32 (0) Begonialaan 3a F NL +31 (0) F BE +32 (0) TE Huissen E info@bio-connect.nl The Netherlands W

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3 THE BENEFITS OF AG INHIBITOR COCKTAILS INCLUDE: Effectiveness. Researched in detail using HPLC studies, substrate-based studies, and more. Optimized to maximize the effectiveness of each individual inhibitor within the complex mixture of these inhibitor cocktails. Stability. In general are stable for a few years. Specificity. Designed to provide a comprehensive selection of specific cocktail formulations. Using fewer inhibitors avoids unnecessary contamination due to unwanted inhibitors and keeps costs lower. We also offer Animal-Free, EDTA-Free, and more for unique applications. Cost. Pricing 20-50% lower than similar competitor products available in the market. Form. Lyophilized solids allow instantaneous dissolution. Liquids based on mode-of-action and solubility properties make them readily available for instantaneous inhibitory action with target enzymes. Competitors products such as compressed tablets require time, effort and undesired delay for dissolution. Quality. Consistent high quality, making your application results highly reproducible. ISO 9001:2008 approved facility. Customize. Don t see the exact combination you require? We regularly produce unique inhibitor combinations in a variety of sizes. Inquire with your special requirements and we ll employ our extensive experience in creating the ideal custom inhibitor cocktail. 1

4 Protease And Phosphatase Inhibitor Cocktail Frequently Asked Questions Q: My lab needs an inhibitor cocktail not represented in this catalog, but it is for small-scale use. Can AG Scientific make it for me? A: Yes. We will work with you to develop a custom formulation of inhibitors for your specific research needs. Our scientists are readily available to assist you with developing an inhibitor cocktail with only the specific inhibitors your research requires. Q: I m not sure which inhibitor cocktail is best for my research. Would it be better to order a wideranging cocktail instead of designing a custom formulation? A: In general, no. Including more inhibitors than necessary can have detrimental effects on protein activity. Additionally these additives can cause contamination and will cost more than specifying the necessary inhibitors for your job. A.G Scientific offers expertise beyond your lab. We have collaborative services that can assist you in developing your cocktail, saving you time and money and eliminating unnecessary trial and error. Q: In the past, we have always ordered bulk supplies from the largest suppliers in the country, but they are more difficult to work with when it comes to custom formulations. Do you have the same quality and competitive prices? A: Absolutely. In fact, we are able to offer better prices than our competitors, at 20%-50% below prices of similar products available on the market. We pass our lower overhead savings on to you. We also have numerous bulk clients not using cocktails, but ordering large scales of one or two inhibitors. AG Scientific specializes in bulk accounts, but we are never too busy to listen to our customers needs. Q: What are the best ways to optimize the life of my proteins? A: Each inhibitor or inhibitor cocktail comes with an informational insert describing the best ways to handle each particular reagent. AG Scientific is constantly looking for ways to increase preservation of protein samples, and we pass along our knowledge to you. We also provide rapid shipping and easy-touse formulations to ensure the most stable products with the quickest activity. Q: What are the advantages of the physical form of your cocktails compared to the compressed solids we normally receive? A: Whereas compressed solids take time and effort (i.e. vortexing, heating, stirring) to solubilize, our cocktails come in the form of lyophilized powder that readily dissolve into solution. Proteolysis is time sensitive, and delayed solubilization causes preventable protein denaturation. Instant lyophilized powder dissolution allows for quick enzyme inhibition to preserve as much protein from degrading as possible. Q: I need a supply to deliver to my client. Are you an OEM? Do you offer private labeling services? A: We provide the entire range of services from manufacture, lyophilization, packaging, private labeling, custom inserts and more. We have over 20 years of development and production experience. 2

5 Protease Inhibitor Cocktails Invasive proteases can contaminate research samples and slow or halt research progress, rendering timeconsuming and expensive work useless. Protease inhibitor cocktails are a unique series of enzyme inhibitors that knock out specific proteases to avoid peptide bond hydrolysis and subsequent protein destruction. Protease inhibitors are vital to scientists engaged in proteomic research, protein expression, characterization, protein modification, protein profiling, and quantitative measurement of proteins to prevent destructive protein degradation. A.G Scientific maintains a large selection of standard inhibitor cocktails in stock, as well as the ability to prepare custom formulations. Available protease inhibitor cocktails: Product Code Product Name Components Specificity of Inhibition P-1510 Protease Inhibitor Cocktail I AEBSF, Aprotinin, E-64, EDTA, Leupeptin P-1540 Protease Inhibitor Cocktail I, Animal-Free AEBSF, Aprotinin (recombinant), E-64, EDTA, Leupeptin P-1511 Protease Inhibitor Cocktail II AEBSF, bestatin, E-64, EDTA, pepstatin A P-1512 Protease Inhibitor Cocktail III P-1542 P-1543 Protease Inhibitor Cocktail III, Animal-Free Protease Inhibitor Cocktail III, Animal-Free, DMSO-Free AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A AEBSF, aprotinin (recombinant), bestatin, E-64, leupeptin, pepstatin A AEBSF, aprotinin (recombinant), bestatin, E-64, leupeptin, pepstatin A P-1513 Protease Inhibitor Cocktail IV AESBF, E-64, Pepstatin A, Phenanthroline P-1514 P-1544 Protease Inhibitor Cocktail V, EDTA-free Protease Inhibitor Cocktail V, EDTA-free, animal-free AESBF, Aprotinin, E-64, Leupeptin AESBF, Aprotinin (recombinant), E-64, Leupeptin esterases; metalloproteases esterases; metalloproteases Serine, cysteine, and aspartic proteases; aminopeptidases; metalloproteases Serine, cysteine, aspartic, and trypsin-like proteases; aminopeptidases Serine, cysteine, aspartic, and trypsin-like proteases; aminopeptidases Serine, cysteine, aspartic, and trypsin-like proteases; aminopeptidases Serine, cysteine, and aspartic proteases; metalloproteases Serine, cysteine, and trypsin-like proteases Serine, cysteine, and trypsin-like proteases P-1515 Protease Inhibitor Cocktail VI, General Use AEBSF, aprotinin, bestatin, E-64, EDTA, leupeptin aminopeptidases; metalloproteases P-1545 Protease Inhibitor Cocktail VI, Plant Cells AEBSF, bestatin, E-64, leupeptin, phenanthroline, pepstatin A Serine, cysteine, and aspartic proteases; amiopeptidases; metalloproteases P-1546 Protease Inhibitor Cocktail VII AEBSF, bestatin, E-64, pepstatin A, phosphoramidon Serine, cysteine, and aspartic proteases; aminopeptidases; metalloendopeptidases P-1547 Protease Inhibitor Cocktail VII, DMSO-free AEBSF, bestatin, E-64, pepstatin A, phosphoramidon P-1548 Protease Inhibitor Cocktail VIII ALLN, antipain, E-64 P-1516 Serine Protease Inhibitor Cocktail I P-1551 PIA 100x, Protease Inhibitor A AEBSF, aprotinin, elastatinal, GGACK Antipain, leupeptin, 50 mg/ml EDTA in water Serine, cysteine, and aspartic proteases; aminopeptidases; metalloendopeptidases Calpain I/II, cathepsin A/B, cathepsin L/S, papain, cysteine proteases Cymotrypsin, kallikrein, plasmin, thrombin, trypsin, elastase, urokinase, Factor Xa Ca2+-dependent endopeptidases, papain, trypsin-like serine and cysteine proteases P-1552 PIA 1000x, Protease Inhibitor A Antipain, leupeptin, 50 mg/ml EDTA in water Ca2+-dependent endopeptidases, papain, trypsin-like serine and cysteine proteases 3

6 Phosphatase Inhibitor Cocktails Phosphatases play an important role in many signal transduction pathways, as the addition or removal of a phosphate group can activate or de-activate an enzyme, or allow a particular protein-protein interaction. Phosphatase inhibitors vary in function, targeting specific unwanted phosphatases to prevent their activity. Combinations of inhibitors in cocktail form can selectively target particular contaminants. AG Scientific maintains a selection of standard inhibitor cocktails in stock, and retains the ability to prepare custom formulations. Available Phosphatase Inhibitor Cocktails: Product Code Product Name Components Specificity of Inhibition P-1517 Phosphatase Inhibitor Cocktail I P-1518 Phosphatase Inhibitor Cocktail II- Solid/Solution P-1549 Phosphatase Inhibitor Cocktail III P-1550 Phosphatase Inhibitor Cocktail IV Bromotetramisole, cantharidin, microcystin LR Imidazole, Na(orthovanadate, fluoride, tartrate, molybdate) Na(orthovanadate, fluoride, pyrophosphate), glycerophosphate Bromotetramisole, cantharidin, calyculin A Alkaline phosphatases, protein phosphatase 1/2A (PP-1/PP-2A) Protein tyrosine phosphatase, acid phosphatases, alkaline phosphatases Ser/Thr phosphatases, acid phosphatases, alkaline phosphatases, PP-1/PP-2A Alkaline phosphatases, PP-1/PP-2A 4

7 Turbocharged Protease/Phosphatase Inhibitor Cocktails Turbocharged Protease/Phosphatase Inhibitor Cocktail is a broad spectrum cocktail, specifically designed to provide complete protection of valuable proteins of interest from degradation during extraction and purification. Able to dissolve instantaneously, our cocktails are lyophilized solids which are readily available for immediate inhibitory action. Our cocktails are much easier to use than tablets, which require splitting, vortexing and timeconsuming dissolution prior to use. Our lyophilized solids are guaranteed for stability, reproducibility and have a shelf life of over a year. Most other commercially available cocktails made and sold in aqueous solutions offer very low guarantee of stability after 1 month of being manufactured. Available Protease/Phosphatase Inhibitor Cocktails: Product Code Product Name Components Specificity of Inhibition T-2492 Turbocharged Protease Inhibitor Cocktail AEBSF HCl, Aprotinin, Bestatin, Pepstatin, E-64, EDTA, Leupeptin, Antipain. esterases; metalloproteases T-2493 Turbocharged Protease Inhibitor Cocktail, EDTA Free AEBSF HCl, Aprotinin, Bestatin, Pepstatin, E-64, EDTA, Leupeptin, Antipain. esterases; metalloproteases T-2494 Turbocharged Phosphatase Inhibitor Cocktail Imidazole, Sodium orthovanadate, Sodium fluoride, Sodium tartrate dihydrate, Sodium molybdate, Sodium Pyrophosphate, Glyserophosphate, Bromotetramisole Oxalate. Protein tyrosine phosphatase, Acid phosphatases, Alkaline phosphatases, PP-1/ PP-2A, Acid and phosphoprotein phosphatases, Ser/Thr Phosphatases T-2495 Turbocharged Protease+Phosphatase Inhibitor Cocktail AEBSF HCl, Aprotinin, Bestatin, Pepstatin, E-64, EDTA, Leupeptin, Antipain, Imidazole, Sodium orthovanadate, Sodium fluoride, Sodium tartrate dihydrate, Sodium molybdate, Sodium Pyrophosphate, Glyserophosphate, Bromotetramisole Oxalate. esterases; metalloproteases, Protein tyrosine phosphatase, Acid phosphatases, Alkaline phosphatases, PP-1/PP-2A, Acid and phosphoprotein phosphatases, Ser/Thr Phosphatases T-2496 Turbocharged Protease+Phosphatase Inhibitor Cocktail, EDTA Free. AEBSF HCl, Aprotinin, Bestatin, Pepstatin, E-64, EDTA, Leupeptin, Antipain, Imidazole, Sodium orthovanadate, Sodium fluoride, Sodium tartrate dihydrate, Sodium molybdate, Sodium Pyrophosphate, Glyserophosphate, Bromotetramisole Oxalate. esterases; metalloproteases, Protein tyrosine phosphatase, Acid phosphatases, Alkaline phosphatases, PP-1/PP-2A, Acid and phosphoprotein phosphatases, Ser/Thr Phosphatases 5

8 Individual Inhibitors All inhibitors are available independently, in custom formulations, or in tested and researched combinations of application-specific cocktails. AG Scientific maintains an extensive selection of specific cocktail formulations designed to inhibit destructive activity in most tissues or cell extracts including mammalian, bacterial, yeast, fungal, and plant cells. For specific research needs, we are readily available to help you develop a custom inhibitor cocktail. Inhibitor Acetyl-Pepstatin AEBSF Alafosfalin Amastatin HCl Antipain Antipain 2HCl Aprotinin Description An aspartyl protease inhibitor. Serine proteases such as thrombin, plasmin, kallikrein, chymotrypsin, and trypsin. Also inhibits TGFβ (transforming growth factor beta), moderately inhibits CD26 (Dipeptidyl peptidase IV: DPP IV) An antibacterial agent Isolate from the culture filtrate of Streptomyces sp., Amastatin is a slow, tightbinding inhibitor of aminopeptidases. It does not inhibit aminopeptidase B. Amastatin has been utilized to inhibit degradation by aminopeptidases, resulting in the potentiation of bioactive peptide activity Papain and trypsin, and to a smaller extent plasmin Inhibits papain and trypsin and to a small extent plasmin. Antipain is more specific for papain and trypsin than is leupeptin. The inhibitory potency of antipain is 100- fold higher than that of elastatinal. Acts by forming a hemiacetal adduct between its aldehyde group and the active serine of the proteinase Kallikrein, trypsin, chymotrypsin, and various serine proteases Bestatin Aminopeptidase and Leukotriene A4 hydrolase (LTA4H). Boc-statine A low molecular weight inhibitor Calyculin A Protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A-C) Cantharidin Cathepsin Inhibitor I Dipeptidylpeptidase IV Inhibitor E-64 E-64 c E-64 d Elastatinal Elastase Inhibitor I Protein phosphatase 2A Cell-permeable cysteine protease inhibitor Dipeptidyl peptidase IV inhibitor. Inhibits rat, canine, human and monkey enzymes with ICvalues of 3, 5, 8, and 8 nm respectively. Long-acting hypoglycemic agent. Beneficial as protein preservation stabilization inhibitor. A potent calpain inhibitor and a membrane-impermeable analog of E-64 of which displays a better inhibition activity against cathepsins B and L than E-64 A potent calpain inhibitor and a membrane-impermeable analog of E-64 of which displays a better inhibition activity against cathepsins B and L than E-64 An irreversible, highly selective cysteine protease inhibitor and a cell-permeable analog of E-64 but with the difference that E-64 d has no charged groups. Has been used in research studies of muscular dystrophy Phenylmethylsulfonyl fluoride (PMSF, sc-3597) degradation of the H1 histone and high mobility group (HMG) proteins. Also inhibits Neutrophil Elastase Isolate from the culture filtrate of Streptomyces sp., Amastatin is a slow, tightbinding inhibitor of aminopeptidases. It does not inhibit aminopeptidase B. Amastatin has been utilized to inhibit degradation by aminopeptidases, resulting in the potentiation of bioactive peptide activity 6

9 Inhibitor Epiamastatin HCl Epibestatin HCl FALGPA GGACK Leupeptin Leupeptin HCl Leupeptin Hemisulfate Leupeptin TFA Pepstatin A Phosphoramidon Phosphoramidon disodium salt Propionyl-leupeptin hemisulfate salt Description A metallo-protease inhibitor showing a selectivity for aminopeptidases. Epiamastatin is pharmacologically inactive compared to Amastatin and differs only by a single transposition of an OH group A metallo-protease inhibitor showing a selectivity for aminopeptidases Substrate for collagenase for the use in a continuously recoding spectrophotometric assay reducing the adsoprtion range to nm Urokinase(uPA), Factor Xa, Factor IX, Factor VII and Trypsin Serine and cysteine proteases. Often used for inhibition of plasmin, trypsin, papain, kallikrein, and cathepsin B. Blocks various apoptotic pathways in T cells A potent, reversible inhibitor of serine and cystein proteases. It showed little to no inhibition activity against: pepsin, cathepsins A and D and alpha-chymotrypsin. All three salt forms are equally effective, when adjusted to their molarity A potent, reversible inhibitor of serine and cystein proteases. It showed little to no inhibition activity against: pepsin, cathepsins A and D and alpha-chymotrypsin. All three salt forms are equally effective, when adjusted to their molarity A potent, reversible inhibitor of serine and cystein proteases. It showed little to no inhibition activity against: pepsin, cathepsins A and D and alpha-chymotrypsin. All three salt forms are equally effective, when adjusted to their molarity Aspartic proteases. Pepsin, cathepsin D, rennin, chymosin, HIV proteases and Fcγ receptor induction by IFN-γ CD10 (neutral endopeptidase 24.11, NEP), metalloproteases (MMPs), Neprilysin-2 (NEP2) and endothelial converting enzyme (ECE) A potent, reversible inhibitor of metallo-proteases. Inhibition of some metalloendopeptidases A potent, reversible inhibitor of metallo-proteases. Inhibition of some metalloendopeptidases Subtilisin Inhibitor I Inhibition of serine proteases TLCK A specific, highly effective, irreversible inhibitor of lysyl endopeptidase. It showed no inhibition of chymotripsin. Activated caspase proteases can be inhibited with TLCK 7

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