Lignin and structural and non-structural carbohydrates and their association with stem strength components of irrigated spring wheat

Transcription

1 Lignin and structural and non-structural carbohydrates and their association with stem strength components of irrigated spring wheat 3 rd Internacional de Trigo (Wheat) Yield Potential Workshop de marzo de 2017 Ciudad Obregón, Sonora, México Francisco Piñera, Pete Berry, John Foulkes, Gemma Molero and Matthew P. Reynolds

2 Lodging Farmers field, March 2011 Selections at Toluca, September 2016 CENEB, March 2014 CENEB, March 2016 Farmers field, March 2017

3 Biomass composition and lodging Literature review Wheat straw biomass consists mainly of lignocellulosic compounds (Harper and Linch 1981, Collins et al. 2014) Lignin Ma (2009), Knapp gene et TaCM al. (1987) (involved concluded in lignin that biosynthesis) is associated arrangement with stem strength and inter-relationships and lodging index of the lignin and structural carbohydrates in Peng et al., wheat (2014), cell growth walls may regulators be more alter important lignin accumulation and the strength than of their basal simple internodes concentrations Wiersma et al. (2011) found a positive association between lodging resistance and lignin Cellulose Wang et al. (2012) proposed that cellulose plays a more important role in the ability of wheat stems to resist lodging

4 Objective The main goal was to investigate the contributions of structural biomass components (lignin, cellulose and hemicellulose) and WSC to stem strength.

5 Materials and Methods Field experiments established during the field seasons , and Ten cultivars from the CIMCOG panel WSC content (dry weight basis) CIMMYT s Maize Nutrition Quality and Plant Tissue Analysis Laboratory Anthrone method (Galicia et al., 2008) Lignin, cellulose and hemicellulose content (dry weight basis) Bioenergy and Brewing Science Building laboratories of the University of Nottingham (Sutton Bonington, UK). Lignin, Acetyl Bromide Spectrophotometric Method (Fukushima and Hatfield, 2001) Cellulose and hemicellulose fractions were estimated according to the method described by Fang et al. (1999)..

6 Cultivars with contrasting overall stem strength No. Name SS class SS (N mm)* 1 BACANORA T 88 Low BCN/RIALTO Low PFAU/SERI.1B//AMAD/3/WAXWING (=Super 152) Low CMH79A.955/4/AGA/3/4*SN64/CNO67//INIA66/5/NAC/6/RIALTO Intermediate CROC_1/AE.SQUARROSA (205)//BORL95/3/PRL/SARA//TSI/VEE#5/4/FRET2 Intermediate SIETE CERROS T66 (Hist 1) Intermediate TACUPETOF2001/BRAMBLING*2/5/KAUZ//ALTAR84/AOS/3/MILA N/KAUZ/4/HUITES WBLL1*2/KURUKU*2/5/REH/HARE//2*BCN/3/CROC_1/AE.SQUA RROSA(213)//PGO/4/HUITES YAV_3/SCO//JO69/CRA/3/YAV79/4/AE.SQUARROSA(498)/5/LINE 1073/6/KAUZ*2/4/CAR//KAL/BB/3/NAC/5/KAUZ/7/KRONSTAD F2004/8/KAUZ/PASTOR//PBW343 Intermediate 183 High 208 High CNO79//PF70354/MUS/3/PASTOR/4/BAV92*2/5/FH6-1-7 High 229 * Means from cross-year analysis for stem strength (SS) of internode 2 (Piñera-Chavez et al., 2016, Field Crops Research).

7 Biomass composition of stem base Stem strength Cultivar WSC (%) Lignin (%) Cellulose (%) Hemicellulose (%) Low Mean Intermediate Mean High Mean Grand Mean Experiment (SED) 0.87***(2) 0.62***(2) 1.24***(1) 1.40***(1) Cultivar (SED) 1.56***(9) 1.11***(9) 1.96***(9) 2.21*(9) Interaction (SED) 2.19ns(13) 1.55**(13) 2.90**(9) 3.28ns(9) ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant

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9 Stem strength and biomass components Stem strength Stem material strength

10 Regression equations for relationships between stem strength and biomass components Lignin WSC Cellulose Hemicellulose Stem strength Stem material strength Equation R 2 Equation R y = 9.21x y = -1.08x y = -3.45x y = -1.64x y = -9.78x (P < 0.05) y = 2.45x Mean y = -2.99x y = -1.40x y = -1.15x y = -1.07x y = 0.87x y = 0.82x y = 2.96x y = -2.38x Mean y = -1.61x y = -0.36x y = -1.98x y = 1.49x (P < 0.01) 2013 y = -1.89x y = 0.009x Mean y = -0.71x y = 1.54x (P = 0.10) 2012 y = -0.68x y = 0.72x y = 8.14x y = 2.99x Mean y = 3.53x y = 1.84x

11 Stem strength (N mm) Structural biomass and stem strength Dry weight per length (mg mm -1 )

12 Conclusions This study illustrated that wheat genotypes with a range of stem strength dimensions differed in biomass composition (lignin, cellulose, hemicellulose and WSC) within and across environments. However, a comparison of low, intermediate and high stem strength genotypes indicated similar concentrations of biomass components Lignin and cellulose concentrations were affected by the environment. Variation on the stem strength of wheat will depend on the biomass composition and interaction of its components

13 Acknowledgements

14 Thanks!