2017/18 PRODUCT CATALOGUE SCHIZOSACCHAROMYCES POMBE
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1 2017/18 PRODUCT CATALOGUE SCHIZOSACCHAROMYCES POMBE
2 CONTENTS 3 Schizosaccharomyces Pombe 4 Complex Media 5 YE Broth 5 YE Agar 6 YES Broth 7 YES Agar 8 YSO Broth 9 YSO Agar 10 ME Broth 11 ME Agar 22 SP Supplements 22 SP Supplements 23 SP Supplements, -Leu, -Ura 23 SP Supplements, -Lys 24 Schizosaccharomyces Pombe Minimal Defined Media Technical Data 12 Minimal Defined Media 2 12 EMM Agar 13 EMM Broth 13 EMM Agar without Dextrose 14 EMM Broth without Dextrose 14 EMM Agar low Dextrose, 5 g/l 15 EMM Broth low Dextrose, 5 g/l 15 EMM Agar plus 1 x SP Supplements, -Leu, -Ura 16 EMM Broth plus 1 x SP Supplements, -Leu, -Ura 16 EMMS 17 EMM Agar without Phosphates 17 EMM Broth without Phosphates 18 EMM Agar without Nitrogen 18 EMM Broth without Nitrogen 19 EMM Glutamate Agar 19 EMM Glutamate Broth 20 MB Agar 20 MB Broth 21 MB Broth without Amino acids 21 MMA Agar Medium
3 SCHIZOSACCHAROMYCES POMBE Besides Saccharomyces Cerevisiae, Schizosaccharomyces Pombe, a fission yeast, is the other commonly studied yeast. Schizosaccharomyces Pombe is different from Saccharomyces Cerevisiae in a way that it has a typical eukaryotic cell cycle with discrete G1, G2 and M phases. Another difference is it's chromosome structure and RNA processing that are much closer to the systems present in mammalian cells compared to Saccharomyces Cerevisiae. Schizosaccharomyces Pombe is isolated from Pombe (an East African Millet beer). The size of it's genome is about 14 Mb containing approximately 7000 genes arranged in three distinct genomes. Schizosaccharomyces Pombe has two mating types, h+ and h-, and is stable as a haploid or diploid. The most commonly auxotrophic markers used with Schizosaccharomyces Pombe are; Ade, Glu, His, Leu, Lys and Ura. Homologous recombination of Schizosaccharomyces Pombe is less efficient compared to Saccharomyces Cerevisiae. Conjugation and sporulation can only take place when starved for nutrients. Formedium is producing a large range of media for the cultivation of Schizosaccharomyces Pombe. Fission yeast like Schizosaccharomyces Pombe can can be grown on Saccharomyces Cerevisiae media like YPD for general culturing purposes, however, the cells are not as happy on Peptone (the P in YPD). Therefore YPD (Yeast Extract, Peptone and Dextrose) is often replaced by media like YES in which Peptone is omitted or YSO in which Peptone is replaced by Casamino Acids. Many of the Schizosaccharomyces Pombe media contain 30 gram/litre of Dextrose (glucose.h2o) compared to the Saccharomyces Cerevisiae media with 20 gram/litre of Dextrose. To prevent caramelization of Glucose during autoclavation it is essential to mix the powdered media in for 5 minutes in order to dissolve the Glucose completely. Autoclavation is recommended at 15 psi for 15 minutes or 10 psi for 20 minutes. Filter sterilization is a good alternative as well. 3 Formedium is producing four groups of media formulations for the cultivation of Schizosaccharomyces Pombe. S. Moreno, A. Klar, and P. Nurse. (1991). Molecular genetic analysis of the fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194: Nasim, A., Young, P. and Johnson, B.F., eds. (1989) Molecular Biology of the Fission Yeasts. Academic Press Inc., NY.
4 COMPLEX MEDIA Schizosaccharomyces Pombe Complex Media This product category includes: 5 YE Broth 5 YE Agar 6 YES Broth 7 YES Agar 8 YSO Broth 9 YSO Agar 10 ME Broth 11 ME Agar 4
5 YE BROTH PCM0102 PCM0105 PCM0110 PCM0150 PCM g 15kg (Yeast extract, Glucose) Formula g/l Yeast extract 5 Dextrose 30 Total 35 Suspend gram powdered medium in 1 litre distilled Store dry at room temperature YE AGAR 5 PCM0202 PCM0205 PCM0210 PCM0250 PCM g 15kg (Yeast extract, Glucose and Agar) Formula g/l Yeast extract 5 Dextrose 30 Agar 17 Total 52 Suspend gram powdered medium in 1 litre distilled Store dry at room temperature
6 YES BROTH PCM0302 PCM0305 PCM0310 PCM0350 PCM g 15kg (Yeast extract, Glucose, Amino acid supplement) Formula g/l 6 Yeast extract 5 Dextrose 30 Adenine 0.05 L-Histidine 0.05 L-Leucine 0.05 L-Lysine HCl 0.05 Uracil 0.05 Total Suspend gram powdered medium in 1 litre distilled Store dry at room temperature
7 YES AGAR PCM0402 PCM0405 PCM0410 PCM g (Yeast extract, Glucose, Agar, Amino acid supplement) Formula g/l Yeast extract 5 Dextrose 30 Agar 17 Adenine 0.05 L-Histidine 0.05 L-Leucine 0.05 L-Lysine HCl 0.05 Uracil 0.05 Total Suspend gram powdered medium in 1 litre distilled Store dry at room temperature 7
8 YSO BROTH PCM0502 PCM0505 PCM0510 PCM g (Casamino acids, Glucose, Amino acid supplement w/o Adenine) Formula g/l 8 Dextrose 30 Casamino acids 2 L-Histidine 0.05 L-Leucine 0.05 L-Lysine HCl 0.05 Uracil 0.05 Total Suspend gram powdered medium in 1 litre distilled Store dry at room temperature
9 YSO AGAR PCM0602 PCM0605 PCM0610 PCM g (Casamino acids, Glucose, Agar, Amino acid supplement w/o Adenine) Formula g/l Dextrose 30 Casamino acids 2 Agar 17 L-Histidine 0.05 L-Leucine 0.05 L-Lysine HCl 0.05 Uracil 0.05 Total Suspend gram powdered medium in 1 litre distilled Store dry at room temperature 9
10 ME BROTH PCM0702 PCM0705 PCM0710 (Malt extract, Amino acid supplement w/o L-Lysine HCl) Formula g/l Malt Extract 30 Adenine 0.05 L-Histidine 0.05 L-Leucine 0.05 Uracil 0.05 Total Suspend gram powdered medium in 1 litre distilled Store dry at room temperature 10
11 ME AGAR PCM0702 PCM0705 PCM0710 (Malt extract, Agar, Amino acid supplement w/o L-Lysine HCl) Formula g/l Malt Extract 30 Agar 17 Adenine 0.05 L-Histidine 0.05 L-Leucine 0.05 Uracil 0.05 Total Suspend 47.2 gram powdered medium in 1 litre distilled Store dry at room temperature 11
12 MINIMAL DEFINED MEDIA 12 Schizosaccharomyces Pombe Minimal Defined Media View technical data on pages This product category includes: 12 EMM Agar 13 EMM Broth 13 EMM Agar without Dextrose 14 EMM Broth without Dextrose 14 EMM Agar low Dextrose, 5 g/l 15 EMM Broth low Dextrose, 5 g/l 15 EMM Agar plus 1 x SP Supplements, -Leu, -Ura 16 EMM Broth plus 1 x SP Supplements, -Leu, -Ura 16 EMMS 17 EMM Agar without Phosphates 17 EMM Broth without Phosphates 18 EMM Agar without Nitrogen 18 EMM Broth without Nitrogen 19 EMM Glutamate Agar 19 EMM Glutamate Broth 20 MB Agar 20 MB Broth 21 MB Broth without Amino acids 21 MMA Agar Medium EMM AGAR PMD0101 PMD0102 PMD0105 PMD0110 PMD0150 PMD g 15kg EMM containing Dextrose, Minerals, Trace elements, Vitamins and Agar without Amino Acids. Suspend 49.3 gram powdered medium in 1 litre distilled
13 EMM BROTH PMD0201 PMD0202 PMD0205 PMD0210 PMD0250 PMD g 15kg EMM containing Dextrose, Minerals, Trace elements and Vitamins without Agar and Amino Acids. Suspend 32.3 gram powdered medium in 1 litre distilled EMM AGAR WITHOUT DEXTROSE 13 PMD0301 PMD0302 PMD0305 PMD0310 EMM containing Minerals, Trace elements, Vitamins and Agar without Dextrose and Amino Acids. Suspend 29.3 gram powdered medium in 1 litre distilled
14 EMM BROTH WITHOUT DEXTROSE PMD0401 PMD0402 PMD0405 PMD0410 EMM containing Minerals, Trace elements, Vitamins without Dextrose, Agar and Amino Acids. Suspend 12.3 gram powdered medium in 1 litre distilled 14 EMM AGAR LOW DEXTROSE, 5 G/L PMD0501 PMD0502 PMD0505 PMD0510 EMM containing Dextrose, Minerals, Trace elements, Vitamins and Agar without Amino Acids. Suspend 34.3 gram powdered medium in 1 litre distilled
15 EMM BROTH LOW DEXTROSE, 5 G/L PMD0601 PMD0602 PMD0605 PMD0610 EMM containing Dextrose, Minerals, Trace elements and Vitamins without Agar and Amino Acids. Suspend 17.3 gram powdered medium in 1 litre distilled EMM AGAR PLUS 1 X SP SUPPLEMENTS, -LEU, -URA 15 PMD0701 PMD0702 PMD0705 PMD0710 EMM containing 0.15 g SP supplements ( Ade, His and Lys), Dextrose, Minerals, Trace elements, Vitamins and Agar. Suspend 49.5 gram powdered medium in 1 litre distilled
16 EMM BROTH PLUS 1 X SP SUPPLEMENTS, -LEU, -URA PMD0801 PMD0802 PMD0805 PMD0810 EMM containing 0.15 g SP supplements ( Ade, His and Lys), Dextrose, Minerals, Trace elements and Vitamins without Agar. Suspend 32.5 gram powdered medium in 1 litre distilled 16 EMMS PMD0910 EMM containing g/l Sorbitol (1.2 M), Dextrose, Minerals, Trace elements and Vitamins without Agar and Amino Acids. Suspend gram powdered medium in 1 litre distilled
17 EMM AGAR WITHOUT PHOSPHATES PMD1001 PMD1002 PMD1005 PMD1010 EMM containing Dextrose, Minerals, Trace elements, Vitamins and Agar without NaH2PO4 and Amino acids. Suspend 47.1 gram powdered medium in 1 litre distilled EMM BROTH WITHOUT PHOSPHATES 17 PMD1101 PMD1102 PMD1105 PMD1110 EMM containing Dextrose, Minerals, Trace elements and Vitamins without NaH2PO4 and Agar. Suspend 30.1 gram powdered medium in 1 litre distilled
18 EMM AGAR WITHOUT NITROGEN PMD1201 PMD1202 PMD1205 PMD1210 EMM containing Dextrose, Minerals, Trace elements, Vitamins and Agar without NH4Cl and Amino acids. Suspend 44.3 gram powdered medium in 1 litre distilled 18 EMM BROTH WITHOUT NITROGEN PMD1301 PMD1302 PMD1305 PMD1310 EMM containing Dextrose, Minerals, Trace elements and Vitamins without NH4Cl, Agar and Amino acids. Suspend 27.3 gram powdered medium in 1 litre distilled
19 EMM GLUTAMATE AGAR PMD2101 PMD2102 PMD2105 PMD2110 PMD2150 PMD2155 5kg 15kg Ammonium Chloride (5 g/l) is replaced by Glutamate (3.75 g/l) as a Nitrogen source in EMM Glutamate Agar. EMM Glutamate Agar contains Dextrose, Minerals, Trace elements, Vitamins and Agar and is without Amino Acids. Suspend 48.0 gram powdered medium in 1 litre distilled EMM GLUTAMATE BROTH 19 PMD1801 PMD1802 PMD1805 PMD1810 PMD1850 PMD1855 1kg 5kg 15kg Ammonium Chloride (5 g/l) is replaced by Glutamate (3.75 g/l) as a Nitrogen source in EMM Glutamate Broth. EMM Glutamate Broth contains Dextrose, Minerals, Trace elements, Vitaminsand is without Amino Acids. Suspend 31.0 gram powdered medium in 1 litre distilled
20 MB AGAR PMD1401 PMD1402 PMD1405 PMD1410 MB containing Dextrose, Minerals, Trace elements, Vitamins, Agar and Amino acids. Suspend 28.9 gram powdered medium in 1 litre distilled 20 MB BROTH PMD1501 PMD1502 PMD1505 PMD1510 MB containing Dextrose, Minerals, Trace elements and Vitamins without Agar. Suspend 11.6 gram powdered medium in 1 litre distilled
21 MB BROTH WITHOUT AMINO ACIDS PMD1701 PMD1702 PMD1705 PMD1710 1kg MB containing Dextrose, Minerals, Trace elements and Vitamins without Agar. Suspend 11.6 gram powdered medium in 1 litre distilled MMA AGAR MEDIUM 21 PMD1601 PMD1602 PMD1605 PMD1610 EMM containing Dextrose, Minerals, Trace elements and Vitamins without NaH2PO4 and Agar. Suspend 33.7 gram powdered medium in 1 litre distilled
22 SP SUPPLEMENTS Schizosaccharomyces Pombe Supplements A basic mixture of Ade, His, Leu, Lys and Ura and it's derived formulations. This product category includes: 22 SP Supplements 23 SP Supplements, -Leu, -Ura 23 SP Supplements, -Lys SP SUPPLEMENTS PSU0101 PSU g Formula g/l Adenine L-Histidine HCl 50 L-Leucine 50 L-Lysine HCl 50 Uracil 50 Suspend 0.25 gram in 1 litre distilled for 1X supplements Add 2-4x for auxotrophs or slower growing strains Store dry at room temperature
23 SP SUPPLEMENTS, -LEU, -URA PSU0201 PSU g Formula g/l Adenine 50 L-Histidine HCl 50 L-Lysine HCl 50 Suspend 0.15 gram in 1 litre distilled for 1X supplements SP SUPPLEMENTS, -LYS 23 PSU0301 PSU g Formula g/l Adenine 50 L-Histidine HCl 50 L-Leucine 50 Uracil 50 Suspend 0.2 gram in 1 litre distilled for 1X supplements Add 2-4x for auxotrophs or slower growing strains Store dry at room temperature
24 SCHIZOSACCHAROMYCES POMBE MINIMAL DEFINED MEDIA TECHNICAL DATA EMM EMM w/o Dextrose EMM-Low Glucose EMMS (1,2 M Sorbitol) EMM w/o Phosphate EMM w/o Nitrogen EMM SP MB Medium MMA Agar Medium Units Carbon Source Dextrose g Glucose g Sorbitol g Amino Acids Adenine mg Histidine mg Lysine mg L-Leucine mg 24 Uracil mg Minerals Phtalic Acid K g Na2HPO g NH4Cl g MgCl2.6H2O g CaCl2.2H2O mg KCl g Na2SO mg KH2PO mg K+ Acetate mg MgSO4.7H mg NaCl mg (NH4)2SO mg Vitamins Panthotenic acid mg Nicotinic acid mg Inositol mg Biotin mg Ca-Pantothenate mg
25 EMM EMM w/o Dextrose EMM-Low Glucose EMMS (1,2 M Sorbitol) EMM w/o Phosphate EMM w/o Nitrogen EMM SP MB Medium MMA Agar Medium Units Trace Elements H3BO mg MnSO4.H2O mg ZnSO4.7H2O mg FeCl3.6H2O mg Na2MoO4.2H2O mg KI mg CuSO4.5H2O mg Citric Acid mg Agar Agar (17) (17) (17) - (17) (17) (17) g 25 Total Total g/l Total (Agar) (49.30) (23.30) (34.30) - (47.10) (44.30) (49.50) - - g/l
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