Ahmed, M.A and Elham A. Badr. Field Crops Research Dept., National Research Centre, Dokki, Cairo, Egypt

Transcription

1 Australian Journal of Basic and Applied Sciences, 3(4): , 2009 ISSN Effect of Bio-and Mineral Phosphorus Fertilizer on the Growth, Productivity and NutritionalValue of Some Chickpea cultivars (Cicer arietinum, L.) in Newly Cultivated Land. Ahmed, M.A and Elham A. Badr Field Crops Research Dept., National Research Centre, Dokki, Cairo, Egypt Abstract: Two field experiments were carried out to study effect of bio-and mineral phosphorus fertilizer on the growth, productivity and nutritional value of some chickpea cultivars (Cicer arietinum L.) in newly cultivated land at New Salheyia Region, Sharkia Governorate. The main results were: Giza-88 cultivar significantly out weighted Giza- 2 cultivar in growth characters at 80 and 95 days (except leaves dry weight and pods dry weight/plant) and yield and its components/ plant and /or fed. (except plant height, number of branches, pods and seeds/ plant). The application of bio-fertilizer named phosphorene in two doses significantly increased growth parameters at 80 and 95 days age (except leaves dry weigh at 80 days after sowing) and yield and its components compared with one dose and control (without bio- P. fertilizer) treatments. The addition of mineral P. fertilizer at rate of 46.5 kg P2O 5/fed. resulted significant increment in growth character of chickpea plants (except branches dry weight/plant) and yield and its components in comparison with 15.5 and 31.0 kg P2O 5/fed treatments. With respect of the between cultivars, bio-p. fertilizer and mineral P.fertlilizer, the between cultivars x Bio-P fertilizer was significant on growth characters and on yield and its components. Moreover, the between cultivars x mineral P. fertilizer rates was significant on growth characters (except leaves dry weight/plant at 80 days age) and yield and its components, whereas, the between bio- P x mineral P. fertilizer rates was significant on growth parameters (except on leaves dry weight/ plant at 80 days) and on the yield and its components. On the other hand, the three way cultivar x phosphorene x P2O 5 was significantly affected growth characters (except leaves dry weight at 80 and 95 days after sowing) and yield and its components except on straw yield/fed. Regarding the chemical composition of chickpea green pods at 100 days after sowing, the effect of cultivars, and /or bio and mineral phosphate fertilizer and their s were studied. Generally, Giza-2 cultivars treated with two doses of bio- P fertilizer and 46.5kg P2O 5/fed produced the highest significant values from leaves dry weight and leaves area/plant at 80 and 95 days age, number of branches; pods and seed/plant at harvest date and N%; protein % ; P%, total soluble sugars % and V.C. in green pods at 100 days, meanwhile, Giza-88 plants treated with two doses of bio-p fertilizer and 46.5 kg/fed P2O 5 harvested the greatest mean values from plant height; number of branches; leaves and pods/plant,branches and pods dry weight/plant at 80 and 95 days age, as well as, plant height; number of branches/plant; weight of pods/plant, and seed and straw yield per plant/or fed at harvest date and K% per green pods at 100 days age. Key words: Chickpea,Phosphorene,mineral phosphorus, growth and Newly cultivated land INTRODUCTION Chickpea is one of the most important legume crops in Egypt as it offers human nutrition with vegetable protein. Many efforts have been made to improve the productivity of chickpea, (Singh et al. 1997, Neelima et al., 1998, Shalaby and El Ashry, 2000, Boulbaba et al, 2005 and Rokhgaid et al, 2008) The yield potential of chickpea can be defined as the total biomass produced or the agricultural important part of the crop. The total biomass is a result of the integration of metabolic reaction in the plant. Consequently, any factor influencing the metabolic activity of the plant at any period of it's growth can affect the yield. Metabolic processes of chickpea plant are greatly governed by both internal, i.e. genetic make up of the plant and external conditions which involve two main factors namely climatic and edaphic environmental factors. The Corresponding Author: Ahmed, M.A, Field Crops Research Dept., National Research Centre, Dokki, Cairo, Egypt 4656

2 yield potential of chickpea could be regulated through alternation of genetical make up and reconstitution of genetical structure through breeding programs and or by modifications of environment through cultural treatments, Thus the objective of this study is to investigate effect of bio- and mineral phosphorus fertilizer on the growth, productivity and nutritional value of some chickpea cultivars in newly cultivated land at new Salheyia Region, Sharkia Governorate. MATERIAL AND METHODS Two field experiments were carried out in newly cultivated lands under sandy soil condition at the new Salheyia Region, Sharkia Governorate, during the two successive seasons of 2006/2007 and 2007/2008 to study effect of bio-and mineral phosphorus fertilizer on the growth, productivity and nutritional value of some chickpea cultivars (Cicer arietinum L.) Each experiment included eighteen treatments which were the combination of two chickpea cultivars (i.e. Giza-2 and Giza-88) and nine treatments representing the between three application doses of the bio fertilizers (phosphorene), i.e. without, one dose and two doses with three rates of mineral phosphorus fertilizer, i.e 15.5, 31.0 and 46.5 kg P2O 5/fed. The treatments were arranged in a split- split block design with four replicates, where; chickpea cultivars were distributed in the main plots, meanwhile, phosphorene treatment were accupped in the sub plots and mineral phosphorus treatments distributed in the sub-sub plots. The experimental unit consisted of five ridges, three meter in length and 60cm apart. Seeds of chickpea were obtained from Agriculture Research Centre, Ministry of Agriculture and were th th planted on November 16 and 20 in 2006 and 2007, respectively, 10 cm between hills at a seeding rate of 60kg/fed. Soil characteristics are presented in Table (1). Table 1: Mechanical and chemical analysis of soil at experimental sites (Average of 2006 and 2007 seasons). Sand % Sit % Clay Texture ph Organic Available N Available P Available K matter % (P.P.m) (P.P.m) (P.P.m) Sand Normal cultural practices were followed as usual in chickpea fields. Phosphorene was applied as recommended by Ministry of Agriculture (two packages each of 1kg/ fed.) once at sowing or twice, i.e. at sowing and one month later as compared to the control (without). Plant height, number of branches; leaves and pods/plant, and dry weight of branches, leaves and pods/plant in grams were estimated at 80 and 95 days after sowing by harvesting ten guarded plants at random from the 2 middle ridges of every plot. Also, area of leaves (LA) "cm /plant" was computed as Bremner and Taha (1966). Random of ten green pods of each treatment was taken at 100 days after sowing for the chemical analysis purpose, i.e. N, P, K, protein, total soluble sugars and ascorbic acid (vitamin C) determination. N, P and K were determined according to Black (1983), Watanab and Olsen (1965) and Jacksen (1965), respectively. The percentage of crude protein) total soluble sugars and ascorbic acid (Vitamin C) were determined according to A.O.A.C. (1984). At full maturity, plant height, number of branches; pods; and seeds/plant, weight of pods/plant, as well as seed and straw yields per plant were recorded using a sample of ten random guarded plants from the middle ridges of each plot, meanwhile, seed and straw yield per fed.were determined from the total plants of each plot. Combined analysis was made for the two growing seasons according to Snedecor and Cochran (1990). For comparison between means, L.S.D test at 5% level was used. RESULTS AND DISCUSSIONS Growth Characters: A-1-Varietal Differences: Data reported in Table (2) show that there were significant differences between chickpea cultivars in plant height, number of branches, leaves; and pods/ plant, as well as, branches; leaves and pods dry weight/plant and leaves area/ plant after 80 and 95 days from planting date. Moreover, its clear that Giza 88 cultivar exceeded significantly Giza-2 cultivar in all previous growth characters except leaves area/plant. It is worthy to mention that plant height, number of branches and pods/plant, and branches dry weight/plant tended to increase with advance in plant age up to 95 days after sowing, meanwhile, number and dry weight of leaves/ plant as well as leaves area/plant tended to decrease after 80 days from sowing. The differences among chickpea cultivars in growth attributes may be due to the differences in number of nodules formed on the root of the tested cultivar. Consequently, the growth of each cultivar may depended 4657

3 mainly on nitrogen fixation (Tawfic et al., 1991), also, to the differences in partition and migration of photosynthate between cultivars (Ahmed et al., 1997) and the endogenous hormones content (Shalaby and El- Ashry, 2000). It could be mentioned that the results of the cultivar differences in growth characters, herein, are in full agreement with those obtained by Dahiya et al. (1993), Kosgey et al. (1993), Singh et al. (1997), El-Karamany and Bahr (1999), Hafiz (2000), Shalaby and Al-Ashry (2000), Boulbaba et al. (2005) and Rokhzaid et al. (2008). A-2. Effect of Bio-p Fertilizer: Table (2) observed that application of phosphorene improved plant growth expressed as plant height, number of branches; leaves and pods/plant, dry weight of branches; leaves (except at 80 days); and pods /plant and leaves area/plant where significantly affected by bio-fertilizer treatment, meanwhile, the effect on leaves dry weight/plant at 80 days after sowing failed to reach the significant level at 5%. Table 2: Effect of cultivars and bio and/or mineral phosphate fertilizer on the growth characters of chickpea plants( Average of 2006/ 2007 and 2007/2008 seasons) Plant height No of branches No. of leaves No. of pods/ Branches dry Leaves dry Pods dry Leaves area "cm" / plant / plant plant w.t g/plant w.tg/plant w.t. g/plant 2 "cm /plant" Cultivars Giza Giza L.S.D at 5% level Phosphorene Without One dose Two doses L.S.D at 5% level n.s PO kg P2O 5/fed kg P2O kg P2O 5/fed L.S.D at 5% level ns ns Data also indicate that two doses application of phosphorene showed an increament in plant growth than of one dose application treatment. The increment in growth characters were significant compared with one dose and control treatment (without bio-fertilizer). It could be concluded that application of the bio- P fertilizer named phosphorene increased plant growth and dry matter due to that phosphorene enhanced phosphorus solubilization (El-Sheekh, 1997). The effect of bio-fertilizer may be due to the effect of nutrients mobilizing microorganisms which help in availability of metals and increased levels of extractable mineral (El-Karamany et al, 2000). The obtained results agreed with those of Sherif et al. (1997) who mentioned that phosphates dissolving bacteria presses the ability in bring a soluble phosphate in soluble forms secreting organic acids which lower the ph and bring about the dissolution of bonds forms of phosphate and render then available for growing plants. The results of bio-p fertilizer in growth attributes obtained in this study are in agreement with those obtained by El-Sawah et al. (1995), Adam (2002), Ahmed et al. (2003) Ahmed and El-Abagy (2007) and Ahmed et al. (2008). A-3: Effect of Mineral P. Fertilizer: The addition of mineral phosphorus fertilizer at a rate of 46.5kg P2O 5/fed resulted in a significant increase in growth characters of chickpea plants compared with 15.5 and 31.0 kg P2O 5/fed. rates except the increase from 31.0 to 46.5 kg P2O 5/fed showed no significant increase in branches dry weight/ plant at 95 days and leaves dry weight/ plant at 80 days after sowing (Table 2). The positive effect of phosphorus fertilizer on growth characters, herene, may be due to the physiological role of P on the meristematic activity of plant tissues and consequently increasing plant growth, also, its function as a part of enzyme system having a vital role of the synthesis of other food from carbohydrate (Ahmed and El-Abagy; 2007 and Ahmed et al., 2008). It could be concluded that our results are in harmony with those obtained by El-Karamany and Bahr (1999), Hafiz (2004), Jain et al. (2006), Seema et al. (2007), Ahmed and El-Abagy (2007) and Ahmed et al. (2008). A-4 Effect of the Interactions: Table (3) indicate that the between cultivars x bio-fertilizer significantly affected growth parameters, i.e. plant height, number of branches; leaves; and pods/plant, dry weight of each one from 4658

4 branches; leaves and pods/ plant and leaves area/ plant at 80 and 95 days. Generally it could be concluded that the highest values of the previous growth parameters (except leaves dry weight and leaves area/plant) by Giza-88 cultivar treated with Bio-P fertilizer at two doses and this was true at 80 and 95 days, meanwhile, Giza-2 cultivar gave the highest value form leaves dry weight and leaves area/ plant under two doses of bio- P fertilizer. Regarding the between cultivars x mineral fertilizers the effect on all growth characters during the different two stages of growth was significant except leaves dry weight at 80 days after sowing. Moreover, Giza-88 cultivar had the highest significant values of growth attributes under 46.5kg P2O 5/fed treatment, meanwhile, Giza-2 treated with 46.5kg P2O 5/fed produced the greatest leaves dry weight and leaves area/plant values at 80 and 95 days after sowing (Table3). Table 3: Effect of between cultivars x phosphorene, cultivar x P2O 5 and phosphorene x P2O 5 on growth characters of chickpea plants. (Average of 2006/2007 and 2007/2008 seasons). Plant height No of branches No. of leaves No. of pods Branches dry Leaves dry Pods dry Leaves area / plant / plant / plant w.tg/plant w.t. g/plant w.tg/plant 2 " cm /plant" Cultivar x phosphorene Without Giza-2 One dose Two doses Without Giza-88 One dose Two doses L.S.D at % level Cultivar x P2O kg P2O 5/fed Giza kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed Giza kg P2O 5/fed kg P2O 5/fed L.S.D at 5% level n.s Phosphorenex P2O5 : 15.5 kg P2O 5/fed Without 31.0 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed One dose 31.0 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed Two doses 31.0 kg P2O 5/fed kg P2O 5/fed L.S.D at 5% level n.s n.s With respect of bio-p and mineral P fertilizer on growth characters, the effect was significant at 80 and 95 days, except on number of branches/ plant and leaves dry weight/pant at 80 days age. In addition, mineral phosphorus fertilizer at biofertilizer at two doses and applied a rate 46.5kg P2O 5/fed resulted in a significant increase in growth characters of chickpea plants compared with other eight treatments of. It is noteworthy to mention, that the three-way between cultivar x bio-p fertilizer x mineral P. fertilizer significantly affected growth characters of chickpea plants at the different stages of growth except leaves dry weight/plant at 80 and 95 days age. It could be summarized that the results of the three way as the most favourable treatment to produce the highest values from the studied growth characters was Giza -88 cultivar treated with Bio-P fertilizer at two doses and supplied with maximum rate of P2O5 fertilizer except leaves dry weight and area/plant recorded by Giza -2 cultivar under the same treatments of bio and mineral fertilizers (Table 4). B- Nutritional Values of Green Pods: Data in Table (5) indicated that the nutritional value of green pods of chickpea plants is significantly affected by cultivar differences since Giza -2 cultivar significantly exceeded Giza-2 cultivar in N%, protein %, P%, total soluble sugars % and V.C, meanwhile, Giza-88 plants recorded the height insignificant value from K%. Again, data in the same table observed that two doses application from phosphorene produced chickpea green pods characterized by the highest content from the chemical constituents studied compared with without application (control) and one dose of phosphorene treatments. With respect of the mineral phosphorus rate, data illustrated in Table (5), show clearly that supplied chickpea plants with 46.5kg P2O 5/fed. gave the greatest mean value from N%, protein%, P%, total soluble sugars%, and V.C. compared with 15.5 and 31.0 kg P O /fed

5 Table 4: Effect of three-way between chickpea-cultivar x phosphorene x P2O 5 levels on growth characters of chickpea plants at 80 and 95 days after sowing (Average of 2006/2007 and 2007/2008 seasons) cultivars Growth characters Plant height No of No. of No. of pods Branches dry Leaves dry Pods dray Leaves area (cm) branches/ plant leaves/ plant / plant wt g/plant wt g/plant wt g/plant 2 cm /plant Phosphorine P2O Giza - 2 Without 15.5 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed One Dose 15.5 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed Two Dose 15.5 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed Giza - 88 Without 15.5 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed One Dose 15.5 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed Two Dose 15.5 kg P2O 5/fed kg P2O 5/fed kg P2O 5/fed L.S.D at 5% level n.s n.s Table 5: Effect of cultivars and/or bio- and mineral phosphate fertilizer on the chemical composition of chickpea green pods (Average of 2006/2007 and 2007/2008 seasons) Cultivars Phsophorene P2O 5 kg/fed N% Protein% P% K% Total soluble V.C (mg/10g. sugars% fresh wt.) Giza Giza L.S.D at n.s Without One dose Two doses L.S.D at n.s n.s L.S.D at With respect to the between cultivar x phosphorene; the effect was significant on N%, protein and V.C since Giza-2cultivar supplied with two doses from phosphorene produced the highest values from N%, protein%, P%, total soluble sugars% and V.C meanwhile, Giza-88 cultivar treated with two doses from phosphorene gave the greatest K% value (Table 6). With respect of the between cultivars xp2o 5 rates the effect was significant on N%, protein % and V.C and not significant on P%, K% and total soluble sugars, also, Giza-2 cultivar with 46.5 kg P2O 5/fed gave chickpea green pods characterized by its high content form N%, protein%, P%, total soluble sugars% and V.C., whereas the green pods of chickpea with high content from K% collected from Giza-88 plants under 46.5 kg P2O 5/fed.( Table 6) Regarding the between phosphorene x P2O 5, the effect was significant on N%, protein% and V.C, whereas, was not significant on P%, K% and total soluble sugars, since, the signets values from the tested chemical composition of chickpea green pods collected from plants treated with two doses from phosphorene and 46.5kgP2O 5/fed. (Table 6). With respect of the three way cultivars x biofertilizer x mineral fertilizer, data detected in (Table 7) indicated cleacly that the effect on N%, protein % and V.C was significant, meanwhile, on P%; K% and total soluble sugars % failed to reach the significant level at 5%. Generally, Giza-2 cultivar recorded the highest N%, protein%, P%, total soluble sugars% and V.C in the green pods of chickpea at 100 days after sowing under two doses of phophorene and 46.5 Kg P2O 5/fed. On the other hand, Giza-88 cultivar gave green pods characterized by high insignificant K% under two doses of phosphorene and 46.5kg P O /fed. (Table 7). 2 5 C. Yield and its Components : C-1: Cultivar Differences: Data reported in Table (3) observed that chickpea cultivars significantly differed in plant height, number of branches and pods/plant, weight of pods/ plant, number of seeds/plant and seed and straw yields per plant and/or per fed. Data show clearly that Giza-2 cultivar significantly surpassed Giza-88 cultivar in number of branches; pods and seeds/plant, meanwhile, Giza-88 cultivar significantly exceeded Giza -2 cultivar in plant height, weight of pods/plant, and seed and straw yield per plant and/or fed. 4660

6 Table 6: Effect of between cultivar x phosphorenes cultivar P2O 5, and phsophorene x P2O 5 on the chemical composition of chickpea green pods. (Average of 2006/2007 and 2007/2008 seasons). Intoduction N% Protein% P% K% Total soluble V.C (mg/10g. sugars% fresh wt.) Culivar x phsophorene Interaction Without Giza-2 One dose Two doses Without Giza-88 One dose Two doses L.S.D at n.s n.s n.s 0.03 Culivar x P2O kg/fed Giza kg/fed kg/fed kg/fed Giza kg/fed kg/fed L.S.D at n.s n.s n.s 0.04 Phosphorene x P2O kg/fed Without 31.0 kg/fed kg/fed kg/fed One dose 31.0 kg/fed kg/fed kg /fed Two doses 31.0 kg/fed kg/fed kg/fed n.s n.s n.s 0.05 Table 7: Effect of three-way between chickpea cultivar x phosphorene x P2 O 5 (Average of 2006/2007 and 2007/2008 seasons). Intoduction Cultivars Phsophorene P2O N% Protein% P% K% Total soluble V.C (mg/10g. 5 sugars% fresh wt.) Without Giza-2 One dose Tow dose Without Giza-88 One dose Tow dose n.s n.s n.s 0.05 The differences between chickpea cultivar in the production efficiency may be due to the differences in number of nodules formed on the root of the tested cultivar, consequently, the growth of each cultivar may depend mainly on nitrogen fixation (Tawfic et al., 1991), also to the differences in partitioning and migration of photosynthate between chickpea cultivar (Ahmed et al., 1997), and in the content of endogenous hormones (Shalaley and Al-Ashry, 2001). Our results are in full agreement with those obtained by Dahiya et al. (1993), Kesgey et al. (1993), Singh et al. (1997); El-Karamany and Bahr (1999), Hafiz (2000), Shalaby and Al-Ashry (2000), Boulbaba et al. (2005) and Rokhzaid et al. (2008). C-2 Effect of Bio-P-fertilizer: Table (7) observed the application of phosphorene significantly improved plant height, number of branches; pods, and seeds/ plant as well as seed and straw yield per plant and /or per fed by application phosphorene 4661

7 in two doses compared with one dose application and the control treatment (without phosphorene). It could be concluded that application of the bio-p fertilizer named phosphorene increased yield and its components and dry matter due to that phosphorene enhanced phosphorus solubilization (El-Sheekh; 1997). The effect of bio-fertilizer may be due to the effect nutrients mobilizing microorganisms which help in availability of metal and increased levels of extratable minerals (El Karamany et al, 2000). The obtained results are in full agreement with those of Sherif et al. (1997), who indicated that phosphate dissolving bacteria presses the ability to bring a soluble phosphate in soluble forms secreting organic acids which lower the ph and bring about the dissolution of bonds forms of phosphate and render then available for growing plants. The results of bio P.fertilizer on yield and its component obtained by El-Sawah et al. (1995), Adam (2002), Ahmed et al. (2003), Ahmed and El-Abagy (2007) and Ahmed et al. (2008). C-3. Effect of Mineral P. Fertilizer: The addition of mineral P.fertilizer at a rate of 46.5 kg P2O 5/fed. significantly increased all yield and its components studied in this study in comparison with the other two treatment 15.5 and 31.0 kg P2O 5/fed except the difference in straw yield/fed between 31.0 and 46.5 kg P2O 5/fed failed to reach the significant level at 5% level (Table 8). Table 8: Effect of cultivars and/or bio- and mineral phosphate fertilizer on yield and its components of chickpea plant (Average of 2006/2007 and 2007/2008 seasons) Cultivars Phsophorene P2O Plant No of No. of Wt.of No.of Seed Straw Seed Straw 5 kg/fed height branches pods/ pods seed/ yield yield yield yield cm /plant plant g/plant plant g/plant g/plant ton/fed ton/fed Giza Giza L.S.D at Without One dose Two doses L.S.D at L.S.D at The increment in yield and its components regarding supplied chickpea plants with 46.5 kg P2O 5/fed. may by attributed to the physiological role of P on the meristematic activity of plant tissues and consequently increasing plant growth, also; its function as a part of enzyme system having a vital role of the synthesis of other food from carbohydrate. It is worthy to mention that our results are in good agreement with those obtained by El-Karamany and Bahr (1999), Hafiz (2004), Jain et al. (2006), Seema et al. (2007), Ahmed and El-Abagy (2007) and Ahmed et al. (2008). C-4 : Effect of the Interaction: Table (9) indicate that yield and its components of chickpea plants significantly affected by the between cultivars x phosphorene. Generally, data illustrated show clearly that Giza-2 cultivar treated with Bio- P. fertilizer at two doses gave the highest significant values from seeds/number of branches; pods and seeds/plant, whereas, Giza-88 cultivar treated with Bio-P. fertilizer at two doses had the greatest significant yields values from plant height, weight of pods/plant, seed and straw yields per plant and straw yield/fed. On the other hand the effect on seed yield/fed was not significant (Table 9). Furthermore, the between cultivars x P2O 5 fertilization rates significantly affected yield and its components of chickpea plants, again, Giza-2 cultivar under 46.5 Kg P2O 5/fed had the greatest significant values from number of branches; pods and seeds/plant, meanwhile, Giza-88 plant gave the highest significant values form plant height, weight of yields/pods/plant and seed and straw yields per plant and/or fed. (Table9)Regarding the Bio-P fertilizer Mineral P. fertilizer, data illustrated in Table (9) indicated that the effect of this on yield and its components was significant, whereas, the highest significant values from yield and its components were collected from plants treated Bio. P. fertilizer at two doses and supplied with 46.5kg P2O 5/fed. With respect of the three-way cultivars x Biofertilizer x Mineral fertilizer; data recorded in table (10) indicate clearly that the effect was significant on yield and its components (except straw yield/fed.). 4662

8 Table 9: Effect of between cultivar x phosphorenes cultivar P2O 5, and phsophorene x P2O 5 on the chemical composition of chickpea green pods. (Average of 2006/2007 and 2007/2008 seasons). Interaction Plant No of No. of Wt.of No.of Seed Straw Seed Straw height branches pods/ pods seed/ yield yield yield yield cm /plant plant g/plant plant g/plant g/plant ton/fed ton/fed Cultivar x phosphorene Without Giza-2 One dose Two doses Without Giza-88 One dose Two doses L.S.D at ns 0.09 Cultivar x P2O kg/fed Giza kg/fed kg/fed Giza kg/fed kg/fed kg/fed L.S.D at phosphorene x P2O kg/fed Without 31.0 kg/fed kg/fed kg/fed One dose 31.0 kg/fed kg/fed kg/fed Two doses 31.0 kg/fed kg/fed L.S.D at Table 10: Effect of three-way between chickpea cultivar x phosphorene x P2O 5 on yield and its components (Average of 2006/2007 and 2007/2008 cultivars). Introduction Cultivars Phsophorene P2O Plant No of No. of Wt.of No.of Seed Straw Seed Straw 5 kg/fed height branches pods/ pods seed/ yield yield yield yield cm plant g/plant plant g/plant g/plant ton/fed ton/fed Without Cultivar One dose Giza-2 Tow dose Cultivar Without Giza-88 One dose Tow dose n.s Generally, Giza-2 cultivar recorded the highest values from number of branches; pods and seeds/plant under two doses of phosphorene and 46.5 kg P2O 5/fed, whereas, Giza -88 cultivar supplied with two doses of phosphorene and 46.5kg P2O 5/fed gave the greatest values from plant height, weight of pods/plant as well as seed and straw yields per plant and /or fed. REFERENCES Adam, S.M., Growth and productivity of Vicia faba plants as influenced by some different bio and chemical nitrogen fertilizer. Egypt. J. Hort, 29:

9 Ahmed, M.A. and H.M.H. El-Abagy, Effect of bio and mineral phosphorus fertilizer on the growth, productivity and nutritional value of some faba bean (Vicia faba L.) cultivars in newly cultivated land. J. of Appl. Sci. Res., 3(6): Ahmed, M.A., H.M.H. El-Abagy and M.S. Hassanein, Effect of bio- and mineral phosphorus fertilizer on the growth, productivity, and nutritional value of some mungbean cultivars (Vigna radiata L. Wilczek) in newly cultivated land. Egypt. J. Appl. Sci., 23(9): Ahmed, M.A., M.S. Hassanein and N.M. Zaki, Yield capacity of some faba bean varieties (Vicia faba L.) J. Appl. Sci., 12: th A.O.A.C., Official Methods of Analysis of the association of Official Analytical Chemists. 14 ed. Association of Official chemists, Washington D.C., U.S.A. Black, C.A., "Methods of soil analysis". Part 1 and II. Amer. Soc. Agron. Inc. Publ. Madison, Wise, U.S.A. BouLababa, L., S. Bou Aziz, Z.M. Mainassara, L. Zourgui and L. Mokhtar, Response of chickpea (Cicer arietinum L.) to potassium fertilization. J. of Agric. Social Sci., 1(1): 7-9. Bremner, P.M. and M.A. Taha, Studies in potato agronomy. 1- The effects of variety, seed size and spacing on growth, development and yield. J. Agric. Sci., 66: Dahiya, S., M. Singh and M. Singh, Relative growth performance of chickpea genotypes to irrigation and fertilizer application. Haryana J. Agron., 9(2): El-Karamany, M.F. and A.A. Bahr, Effect of mineral fertilization, organic manuring and biofertilization on yield and yield components of chickpea (Cicer arietinum L.) cultivars in sandy soil. Egypt. J. Appl. Sci., 14(11): El-Sawah, M.M.A., F.I.A. Hauka and K.H. El-Hamdi, Role of bacterial phytate fertilizers on increasing soil fertility and crop production. Egypt. J. Soil. Sci., 35(3): 311. El-Sheekh, H.M., Effect of bio and mineral phosphate fertilizers on growth, yield and storability of onion. Egypt. J. Appl. Sci., 12(2): 213. Hafiz, S.I., Response of three chickpea cultivars to late foliar spraying with urea as a supplement for early soil applied nitrogen in sandy soils. Ann. Of Agric Sci. Moshtohor, 38(1): Jackson, M.L., Soil Chemical Analysis Advanced Course". Publ. by Auther, Madisoa, Wisconsin, USA. Jain, L.K., P. Singh and J.K. Balyan, Productivity and profitability of chickpea (Cicer aritinum L.) cultivation as influenced by biofertilizers and phosphorus fertilization. Indian J. of Dryland Agric. Res. And Development, 21(2): Kosgey, J.R., B.A. Mckenzie and G.D. Hill, Rhizobium and nitrogen effects on chickpea sown on two dates in Canterbury. Proc. Ann. Conf. Agron. Soc. Of New Zealand, 23: Rokhzadi, A., A. Asgharzadeh, F. Darvish, G. Nour Mohammadi and E. Majidi, Influence of plant growth promoting Rhizobecteria on dry matter accumulation and yield of chickpea (Cicer arietinum L.) under field condition. American-Eurasian J. Agric., Environ. Sci., 3(2): Shalaby Magda, A.F. and Zeinab M. El Ashry, Physiological effect of cycocel (2-chloroethyl trimethyl ammonium chloride) on vegetative growth, flowering and yield of some chickpea cultivars (Cicer arietinum L.). Ann. Of Agric. Sci Moshtohor, 38(4): Seema, S., R.H. Patel and K.M. Gediya, Response of chickpea to FYM and vermicompost with and without PSB and phosphorus nutrition. Res on Crops, 8(3): Sherif, F.A., M.H. Hegazy and F.K. Abdel-Fattah, Lentil yield and its components as affected by bio-fertilization and phosphorus application. J. Agric. Sci. Mansoura Univ., 22(7): Singh, K.B., Chickpea (Cicer arietinum L.). Field Crops Res., 53: th Snedecor, G.W. and W.G. Cochran, Statistical Methods. 8 Ed. Iowa State Univ., Press, Ames Iowa, USA. Tawfic, M.M., M.A. Azzazy and M.A. Mahmoud, Discrepancy response of some soybean cultivars to rhizobial inoculation under newly reclaimed soils. Egypt. J. Agron., 16: Watanab, F.S. and S.R. Olsen, Test of ascorbic acid methods for determining phosphorus and NaHCO extracts for soil. Soil Sci. Soc Amer. Proc., 29: