TRACE ELEMENTS IN SERUM. Event #2, 2010
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- Lawrence Harrington
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1 TRACE ELEMENTS IN SERUM Event #, 00 June 8, 00
2
3 Event #, 00 Serum Aluminum The test materials for serum Al were prepared from human serum obtained from Tennessee Blood Services, Inc. The units were tested by FDA approved methods and found to be Non-reactive for Anti-HIV-/, Anti-HCV.0 and HBsAg. The serum has also been found to STS (RPR) Non-reactive and Negative for HIV- and HCV by PCR. Serum units were dispensed into acid-washed 500-mL polypropylene containers to make up five (5) serum pools. Each pool was spiked with a suite of additional trace elements including aluminum as Al + at various concentrations. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 58:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for serum aluminum range from µg/l (.9 µmol/l) to 07 µg/l (.97 µmol/l). Acceptable ranges for serum aluminum are based on fixed criteria of ±0%, or ±5 µg/l below 5 µg/l. These criteria are based on consensus recommendations from several EQAS organizers (). Discussion. Based on the above criteria, 88.8% of test results reported were judged as satisfactory, with three out of 5 participant laboratories (.0%) reporting or more of the 5 results outside the acceptable ranges.. Taylor, A., Angerer, J., Claeys, F., Kristiansen, J., Mazarrasa, O., Menditto, A., Patriarca, M., Pineau, A., Schoeters, I., Sykes, C., Valkonen, S. and Weykamp, C. Comparison of procedures for evaluating laboratory performance in external quality assessment schemes for lead in blood and aluminum in serum demonstrates the need for common quality specifications. Clinical Chemistry Trace Elements PT Program
4 Serum Aluminum Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l) SE0-06 SE0-07 SE0-08 SE0-09 SE0-0 Robust Mean Robust Standard Deviation Standard Uncertainty RSD (%) Acceptable Range: Upper Limit: Lower Limit: notes: Results reported as less than the method detection limit are excluded from statistical calculations.
5 Serum Aluminum Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method SE0-06 SE0-07 Results (µg/l) SE0-08 SE0-09 SE0-0 Info Only Target Values: ETAAS-Z ETAAS-Z ETAAS-Z Info 56 ICP-MS ETAAS-Z ETAAS-Z ICP-MS DRC/CC-ICP-MS DRC/CC-ICP-MS Info 06 ICP-MS ETAAS-Z ICP-MS Info 0 ETAAS-Z ICP-MS DRC/CC-ICP-MS Info 5 ETAAS-Z 97 9 Info 55 ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ETAAS-Z Info 67 ETAAS-Z Info 0 ICP-AES/OES Info 58 ETAAS Other Percent satisfactory results for all participants: 88.8 % notes: reported outside upper limit Info only: results included for informational purposes only. reported outside lower limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
6 DRC/CC-ICP-MS New York State Department of Health Serum Aluminum Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD ETAAS Other ETAAS-Z ICP-AES/OES ICP-MS All Laboratories Results (µg/l) SE0-06 SE0-07 SE0-08 SE0-09 SE notes: Insufficient data for calculation.
7 Event #, 00 Serum Copper The test materials for serum Cu were prepared from human serum obtained from Tennessee Blood Services, Inc. The units were tested by FDA approved methods and found to be Non-reactive for Anti-HIV-/, Anti-HCV.0 and HBsAg. The serum has also been found to STS (RPR) Non-reactive and Negative for HIV- and HCV by PCR. Serum units were dispensed into acid-washed 500-mL polypropylene containers to make up five (5) serum pools. Each pool was spiked with a suite of additional trace elements including copper as Cu + at various concentrations. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 58:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for serum copper range from 9 µg/l (.65 µmol/l) to 0 µg/l (.80 µmol/l). Acceptable ranges for serum copper are based on fixed criteria of ±5%, or ±95 µg/l below 65 µg/l. These criteria are consistent with those proposed by the OELM Network of EQAS organizers (, ) for trace elements in serum, and are slightly less stringent than those previously suggested for NYS (±0%). Discussion. Based on the above criteria, 8.8% of test results reported were judged as satisfactory, with three of the participant laboratories (.%) reporting or more of the 5 results outside the acceptable ranges.. A. Taylor, J. Angerer, J. Arnaud, F. Claeys, R.L. Jones, O. Mazarrasa, E. Mairiaux, A. Menditto, P.J. Parsons, M. Patriarca, A. Pineau, S. Valkonen, J.-P. Weber and C. Weykamp Accreditation and Quality Assurance J. Arnaud, J.-P. Weber, C.W. Weykamp, P.J. Parsons, J. Angerer, E. Mairiaux, O. Mazarrasa, S. Valkonen, A. Menditto, M. Patriarca, and A. Taylor Clinical Chemistry Trace Elements PT Program
8 Serum Copper Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l serum) SE0-06 SE0-07 SE0-08 SE0-09 SE0-0 Robust Mean Robust Standard Deviation Standard Uncertainty 6 7 RSD (%) Acceptable Range: Upper Limit: Lower Limit: notes: Results reported as less than the method detection limit are excluded from statistical calculations.
9 Serum Copper Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method SE0-06 Results (µg/l serum) SE0-07 SE0-08 SE0-09 SE0-0 Info Only Target Values: DRC/CC-ICP-MS Info 0 ICP-MS ICP-MS ICP-MS Info 56 FAAS ICP-AES/OES ETAAS-Z ICP-MS DRC/CC-ICP-MS ICP-MS FAAS Info 06 ICP-MS ICP-MS Info 05 ICP-MS DRC/CC-ICP-MS Info 5 FAAS Info 60 FAAS ICP-MS ETAAS-Z Info 0 DRC/CC-ICP-MS Info 57 ICP-AES/OES Info Percent satisfactory results for all participants: 8.8 % notes: reported outside upper limit Info only: results included for informational purposes only. reported outside lower limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
10 DRC/CC-ICP-MS New York State Department of Health Serum Copper Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD ETAAS-Z FAAS ICP-AES/OES ICP-MS All Laboratories Results (µg/l serum) SE0-06 SE0-07 SE0-08 SE0-09 SE notes: Insufficient data for calculation.
11 Event #, 00 Serum Selenium The test materials for serum Se were prepared from human serum obtained from Tennessee Blood Services, Inc. The units were tested by FDA approved methods and found to be Non-reactive for Anti-HIV-/, Anti-HCV.0 and HBsAg. The serum has also been found to STS (RPR) Non-reactive and Negative for HIV- and HCV by PCR. Serum units were dispensed into acid-washed 500-mL polypropylene containers to make up five (5) serum pools. Each pool was spiked with a suite of additional trace elements including selenium as Se + at various concentrations. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 58:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for serum selenium range from 0 µg/l (.9 µmol/l) to 99 µg/l (.79 µmol/l). Acceptable ranges for serum selenium are based on fixed criteria of ±0%, or ± µg/l below 0 µg/l. These criteria are a little less stringent than those proposed by the OELM Network of EQAS organizers (±5% or ±8 µg/l below 55 µg/l) (, ) for trace elements in serum. As performance for serum Se improves among NYS-permit laboratories, consideration will be given to adopting the OELM criteria. Discussion. Based on the above criteria, 9.6% of test results reported were judged as satisfactory, with two of the 9 participant laboratories (0.5%) reporting or more of the 5 results outside the acceptable ranges.. A. Taylor, J. Angerer, J. Arnaud, F. Claeys, R.L. Jones, O. Mazarrasa, E. Mairiaux, A. Menditto, P.J. Parsons, M. Patriarca, A. Pineau, S. Valkonen, J.-P. Weber and C. Weykamp Accreditation and Quality Assurance J. Arnaud, J.-P. Weber, C.W. Weykamp, P.J. Parsons, J. Angerer, E. Mairiaux, O. Mazarrasa, S. Valkonen, A. Menditto, M. Patriarca, and A. Taylor Clinical Chemistry Trace Elements PT Program
12 Serum Selenium Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l serum) SE0-06 SE0-07 SE0-08 SE0-09 SE0-0 Robust Mean Robust Standard Deviation Standard Uncertainty RSD (%) Acceptable Range: Upper Limit: Lower Limit: notes: Results reported as less than the method detection limit are excluded from statistical calculations.
13 Serum Selenium Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method SE0-06 Results (µg/l serum) SE0-07 SE0-08 SE0-09 SE0-0 Info Only Target Values: DRC/CC-ICP-MS Info 0 DRC/CC-ICP-MS ICP-MS ICP-MS Info 56 ICP-MS ETAAS-Z ICP-MS DRC/CC-ICP-MS ICP-MS DRC/CC-ICP-MS Info 06 ICP-MS DRC/CC-ICP-MS Info 05 ICP-MS DRC/CC-ICP-MS Info 66 ETAAS-Z Info 67 DRC/CC-ICP-MS Info 0 DRC/CC-ICP-MS Info 0 ETAAS-Z Info 57 ICP-AES/OES Info Percent satisfactory results for all participants: 9.6 % notes: reported outside upper limit Info only: results included for informational purposes only. reported outside lower limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
14 DRC/CC-ICP-MS New York State Department of Health Serum Selenium Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD ETAAS-Z ICP-AES/OES ICP-MS All Laboratories Results (µg/l serum) SE0-06 SE0-07 SE0-08 SE0-09 SE notes: Insufficient data for calculation.
15 Event #, 00 Serum Zinc The test materials for serum Zn were prepared from human serum obtained from Tennessee Blood Services, Inc. The units were tested by FDA approved methods and found to be Non-reactive for Anti-HIV-/, Anti-HCV.0 and HBsAg. The serum has also been found to STS (RPR) Non-reactive and Negative for HIV- and HCV by PCR. Serum units were dispensed into acid-washed 500-mL polypropylene containers to make up five (5) serum pools. Each pool was spiked with a suite of additional trace elements including zinc as Zn + at various concentrations. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 58:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for serum zinc range from 6 µg/l (9.5 µmol/l) to 07 µg/l (5.8 µmol/l). Acceptable ranges for serum zinc are based on fixed criteria of ±5%, or ±5 µg/l below 00 µg/l. These criteria are consistent with those proposed by the OELM network of EQAS organizers () for trace elements in serum. Discussion. Based on the above criteria, 88.% of test results reported were judged as satisfactory, with five out of 7 participant laboratories (8.5%) reporting or more of the 5 results outside the acceptable ranges.. A. Taylor, J. Angerer, J. Arnaud, F. Claeys, R.L. Jones, O. Mazarrasa, E. Mairiaux, A. Menditto, P.J. Parsons, M. Patriarca, A. Pineau, S. Valkonen, J.-P. Weber and C. Weykamp Accreditation and Quality Assurance J. Arnaud, J.-P. Weber, C.W. Weykamp, P.J. Parsons, J. Angerer, E. Mairiaux, O. Mazarrasa, S. Valkonen, A. Menditto, M. Patriarca, and A. Taylor Clinical Chemistry Trace Elements PT Program
16 Serum Zinc Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l serum) SE0-06 SE0-07 SE0-08 SE0-09 SE0-0 Robust Mean Robust Standard Deviation Standard Uncertainty RSD (%) Acceptable Range: Upper Limit: Lower Limit: notes: Results reported as less than the method detection limit are excluded from statistical calculations.
17 Serum Zinc Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method SE0-06 Results (µg/l serum) SE0-07 SE0-08 SE0-09 SE0-0 Info Only Target Values: DRC/CC-ICP-MS Info 0 ICP-MS ICP-MS ICP-MS Info 56 ICP-MS ICP-AES/OES FAAS ICP-MS DRC/CC-ICP-MS ICP-MS FAAS Info 06 ICP-MS FAAS ICP-MS Info 05 ICP-MS DRC/CC-ICP-MS Info 5 FAAS Info 55 ICP-MS ICP-MS ICP-MS FAAS ICP-MS ICP-MS FAAS Info 0 DRC/CC-ICP-MS Info 57 ICP-AES/OES Info 58 FAAS Percent satisfactory results for all participants: 88. % notes: reported outside upper limit Info only: results included for informational purposes only. reported outside lower limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
18 DRC/CC-ICP-MS New York State Department of Health Serum Zinc Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD FAAS ICP-AES/OES ICP-MS All Laboratories Results (µg/l serum) SE0-06 SE0-07 SE0-08 SE0-09 SE notes: Insufficient data for calculation.
19 Event #, 00 Additional Trace Elements Reported in Serum Participant laboratories reported their analytical results for any additional trace elements (other than Al, Cu, Se and Zn) that are routinely reported so that a more complete characterization can be recorded for these PT materials. Results for additional trace elements are reported here, but no target value is implied nor are any acceptable ranges provided. These data are provided solely for educational and informational purposes. In addition to Al, Cu, Se and Zn, the serum pools were supplemented with additional trace elements as indicated below. Additional Elements Mn, Cr, V Trace Elements PT Program
20 Serum Additional Elements, 00 Event # Page Serum Arsenic Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE DRC/CC-ICP-MS <0 <0 <0 <0 <0 Serum Barium Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE ICP-MS <.0 <.0.. <.0 Serum Cadmium Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE DRC/CC-ICP-MS <0.5 <0.5 <0.5 <0.5 <0.5 Serum Cobalt Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE ICP-MS ICP-MS <.0 <.0 <.0 <.0 <.0 Serum Chromium Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE0-0 7 ICP-MS ICP-MS DRC/CC-ICP-MS DRC/CC-ICP-MS <.0 < DRC/CC-ICP-MS Arithmetic Mean, n= SD Serum Iron Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE ICP-AES/OES
21 Serum Additional Elements, 00 Event # Page Serum Manganese Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE DRC/CC-ICP-MS DRC/CC-ICP-MS DRC/CC-ICP-MS ICP-MS DRC/CC-ICP-MS 5 Arithmetic Mean, n= SD Serum Lead Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE DRC/CC-ICP-MS <0. <0. <0. <0. <0. Serum Tin Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE ICP-MS <5.0 <5.0 <5.0 <5.0 <5.0 Serum Tellurium Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE ICP-MS <.0 <.0 <.0 <.0 <.0 Serum Thallium Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE ICP-MS.. <.0 <.0 <.0 Serum Vanadium Results (µg/l) Lab Code Method SE0-06 SE0-07 SE0-08 SE0-09 SE DRC/CC-ICP-MS DRC/CC-ICP-MS
22 ATOMIC SPECTROMETRY METHODS New York State Department of Health Trace Elements in Serum METHOD NOTES A- ETAAS-Z (Electrothermal atomic absorption spectrometry with Zeeman background correction) A- ETAAS other (i.e., D, S-H background correction) A- FAAS (Flame atomic absorption spectrometry) A- CV-AAS (Cold vapor atomic absorption spectrometry) A-5 HG-AAS (Hydride generation atomic absorption spectrometry) A-6 AFS (Atomic fluorescence spectrometry) A-7 Other INDUCTIVELY COUPLED PLASMA P- ICP-MS (Inductively coupled plasma - mass spectrometry) P- DRC/CC-ICP-MS (ICP-MS used in the Dynamic Reaction Cell or Collision Cell mode) P- ICP-AES/OES (ICP atomic/optical emission spectrometry) P- HR-ICP-MS (High resolution ICP-MS) P-5 ETV-ICP-MS (Electrothermal vaporization ICP-MS) P-6 ID-ICP-MS (Isotope dilution ICP-MS) P-7 Other ELECTROCHEMICAL METHODS E- ASV (Anodic stripping voltammetry without digestion) E- ASV-LeadCare (Anodic stripping voltammetry using the ESA LeadCare system) E- Fluoride specific electrode E- Other MOLECULAR FLUORIMETRY F- EtOAc (Ethyl acetate-acetic acid extraction method for determination of erythrocyte protoporphyrin) F- Aviv hematofluorometry (for determination of EP at hematocrit 5) F- Helena ZPP (for determination of zinc protoporphyrin in µmol ZPP/mol heme) F- Other OTHER METHODS If your method is not listed in the above list, please describe it briefly.
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