AAFCO Check Sample 2015 Minerals Program. A Targeted and Engineered Concentration Program
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1 AAFC Check Sample 215 Minerals Program A Targeted and Engineered Concentration Program
2 Approach: 16 Target Elements This quarterly program focuses on minerals of health and toxicological importance in the feed and food chain at significant concentration ranges. Minerals are spiked into the feed or food materials to achieve the desired concentrations that are not available in the AAFC monthly Check Sample Program. Code Element Aluminum Arsenic Boron Cadmium Chromium Cobalt Copper Fluorine Iodine Units Samples for 215: Poultry Layer Feed (Reports 4/3/215) Rice (Reports /31/215) Lead Mercury Molybdenum Nickel ppb Swine Feed or Dairy Feed (Due 1/1/215) 34 Selenium Dog Food (Due 1/1/216) Sulfur Vanadium %
3 Precise final concentration will be unknown due to natural occurrence. We will use a Consensus measure of Location (Robust Estimate). Statistical Approach We will use the Horwitz relationship as a measure of Dispersion. < 2σ H > Z = X LAB X A σ ( Consensus) ModifiedHorwitz Consensus A
4 Modified Horwitz Trumpet 1% 13.8% 1%.1% ppb 1 ppb 1 ppb = 2 mf RSD H C % Analyte Concentration (Mass Fraction) Between Lab %RSD
5 215 Samples Completed to Date Sample # Sample Name # Labs # Methods # Analytes Poultry Layer Feed Rice Sample # Sample Name Compliant Z Warning Z Actionable Z Poultry Layer Feed 5.% 6.8% 18.2% Rice 85.2% 3.% 11.8%
6 Calculated Adjusted Spike as Assigned Value ccasionally the lab data will be wildly dispersed. Even Consensus will not make sense. We need another reference to provide reasonable Z scores. Calculated Adjusted Spike = Base Feed Analyte Value + Spike Added From Poultry Layer Feed Assigned Value = Base Value + Spike Value From PFP Brown Rice Assigned Value = Base Value + Spike Value
7 Spikes in Poultry Layer Feed Code Analyte () # Labs Spike Assigned Value Z Score of Spike Calculated Spike Spike 15 Aluminum 9? Arsenic As 1 mg/ml 1 Boron H 3 B Cadmium Cd 1 mg/ml 52 Chromium CrCl 3 6H 2 21 Cobalt Co 1 mg/ml 22 Copper CuCl 2 2H 2 23 Fluorine NaF 24 Iodine KI 526 Lead Pb(CH 3 C 2 ) 2 3H 2 Mercury (ppb) Hg 1 mg/ml 38 Molybdenum Na 2 Mo 4 2H Nickel NiS 4 6H 2 34 Selenium Se 1 mg/ml 41 Vanadium NaV 3 36 Sulfur (%)
8 Spikes in Rice Code Analyte () # Labs Spike Assigned Value Z Score of Spike Calculated Spike Spike 15 Aluminum 4? 516 Arsenic As 1 mg/ml 1 Boron H 3 B Cadmium Cd 1 mg/ml 52 Chromium CrCl 3 6H 2 21 Cobalt Co 1 mg/ml 22 Copper CuCl 2 2H 2 23 Fluorine NaF 24 Iodine KI 526 Lead Pb(CH 3 C 2 ) 2 3H 2 Mercury (ppb) Hg 1 mg/ml 38 Molybdenum Na 2 Mo 4 2H Nickel NiS 4 6H 2 34 Selenium Se 1 mg/ml 41 Vanadium NaV 3 36 Sulfur (%) NiS 4.6H 2
9 All Targets Will be Above The LQ If you Do NT Detect it, then Do NT Report it. Report sufficient decimal places to include significant digits at the units requested. Report raw data in the required units (35 is.35 % not. %). Do NT report zero s. s are flagged (4) and not used in calculations BUT! You will receive a Z score based on.
10 21551 Minerals Program Analyte Report
11 Proficiency Testing All Tests Report Sample # Minerals Program # Labs Reporting: 13 Poultry Layer Feed Issue Date : 4/3/215 Method Analyte Lab Lab Data Method Values AAFC CS Your Group Group (Units) Code Value Range Rob Mean Horwitz SD R-bar # Labs Z Score Method Flag 2 Protein (%) Fat (%) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Aluminum (mg / kg ()) Boron (mg / kg ()) Boron (mg / kg ()) Boron (mg / kg ()) Boron (mg / kg ()) Boron (mg / kg ()) Calcium (%) Calcium (%) Calcium (%) Calcium (%) Calcium (%) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Page 1 of 5
12 Method Analyte Lab Lab Data Method Values AAFC CS Your Group Group (Units) Code Value Range Rob Mean Horwitz SD R-bar # Labs Z Score Method Flag 21 Cobalt (mg / kg ()) Cobalt (mg / kg ()) Cobalt (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Copper (mg / kg ()) Fluorine (mg / kg ()) Fluorine (mg / kg ()) Fluorine (mg / kg ()) Iodine (mg / kg ()) Iron (mg / kg ()) Iron (mg / kg ()) Iron (mg / kg ()) Iron (mg / kg ()) Iron (mg / kg ()) Iron (mg / kg ()) Magnesium (%) Magnesium (%) Magnesium (%) Magnesium (%) Magnesium (%) Manganese (mg / kg ()) Manganese (mg / kg ()) Manganese (mg / kg ()) Manganese (mg / kg ()) Manganese (mg / kg ()) Manganese (mg / kg ()) Phosphorus (%) Phosphorus (%) Phosphorus (%) Phosphorus (%) Phosphorus (%) Page 2 of 5
13 Method Analyte Lab Lab Data Method Values AAFC CS Your Group Group (Units) Code Value Range Rob Mean Horwitz SD R-bar # Labs Z Score Method Flag 31 Phosphorus (%) Potassium (%) Potassium (%) Potassium (%) Potassium (%) Potassium (%) Potassium (%) Salt as chloride (%) Salt as chloride (%) Selenium (mg / kg ()) Selenium (mg / kg ()) Selenium (mg / kg ()) Selenium (mg / kg ()) Selenium (mg / kg ()) Selenium (mg / kg ()) Selenium (mg / kg ()) Selenium (mg / kg ()) Selenium (mg / kg ()) Sodium (%) Sodium (%) Sodium (%) Sodium (%) Sodium (%) Sodium (%) Sulfur (%) Sulfur (%) Sulfur (%) Sulfur (%) Sulfur (%) Sulfur (%) Sulfur (%) Zinc (mg / kg ()) Zinc (mg / kg ()) Zinc (mg / kg ()) Zinc (mg / kg ()) Zinc (mg / kg ()) Zinc (mg / kg ()) Zinc (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Page 3 of 5
14 Method Analyte Lab Lab Data Method Values AAFC CS Your Group Group (Units) Code Value Range Rob Mean Horwitz SD R-bar # Labs Z Score Method Flag 38 Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Molybdenum (mg / kg ()) Barium (mg / kg ()) Barium (mg / kg ()) Vanadium (mg / kg ()) Vanadium (mg / kg ()) Vanadium (mg / kg ()) Vanadium (mg / kg ()) Vanadium (mg / kg ()) Vanadium (mg / kg ()) Vanadium (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Arsenic, total (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Cadmium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Page 4 of 5
15 Method Analyte Lab Lab Data Method Values AAFC CS Your Group Group (Units) Code Value Range Rob Mean Horwitz SD R-bar # Labs Z Score Method Flag 52 Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Chromium (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Lead (mg / kg ()) Nickel (mg / kg ()) Nickel (mg / kg ()) Nickel (mg / kg ()) Nickel (mg / kg ()) Nickel (mg / kg ()) Nickel (mg / kg ()) Nickel (mg / kg ()) Nickel (mg / kg ()) Notes: Interpreting Z Scores: Red indicates a normally distributed Z value >3 or <-3 (requires action), range = Z between 2 and 3 or -2 and -3 (warning) and Green = Z < 2 and >-2 (K at 95%). Flags indicate data usage: = Used, 1 = Rejected for duplicates too far apart, 2 = Rejected as outlier and 8 = Analyst data exempt. Robust statistics not used if < 6 labs reporting, in this case the Z Scores are included for information only (Grey, No Action!). Flag 9 indicates no statistics calculated for this dataset. To review the problem please see all submitted data for this test. Page 5 of 5
16 Poultry Layer Feed Method Analyte Lab Lab Data Method Values AAFC CS Your Group Group (Units) Code Value Range Spike AV Horwitz SD R-bar # Labs Z Score Method Flag
17 Rice Method Analyte Lab Lab Data Method Values AAFC CS Your Group Group (Units) Code Value Range Spike AV Horwitz SD R-bar # Labs Z Score Method Flag , , , , , ,8.. 2, , , ,
18 As We Move Forward I will keep an eye out for problem Analytes and use the Calculated Adjusted Spike instead of the Robust Assigned Value. In some cases the Calculated Adjusted Spike is our best estimate of the true analyte concentration. We will make efforts to obtain more reliable spike calculations. And tell your friends about our program. We need a lot of participants
19 Questions?
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