W rntroduction . Read about John Endler's experiment . Read the three possible hypotheses for guppy coloration

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1 Sex and the Single Guppy The purpose of this activity is to analyze how guppy populations change over time. The simulation activity allows you to start with a pool of guppies and your choice of predators, you will be able to watch what happens to your guppy population and how the introduction of predators can affect the guppy's appearance. The simulation will help you understand what pressures drive guppy evolution xactivity adapted from PBS - lwww' Pbs.org Open the Guppy Sex Simulator!!! http: / /www. pbs.orq /wg bh /evol utlon'sex /o u ppv/ i ndex. htrnl m@'; W rntroduction First of all, familiarize yourself with the site, you should know each of the following before proceeding to the simulation.. View the guppy gallery, know the color variations found among guppies. View tne [reditor gallery, know the types of predators of guppies -'. Read about John Endler's experiment. View the three types of pools and know what guppy colors are common in each area. Read the three possible hypotheses for guppy coloration

2 Experiment Sexual selection and predation both play roles in the evolution of guppies. Both can affect the coloration of male guppies. In this experiment, you will attempt to answer the following question: QUESTION: To what degree do predation and sexual selection affeet guppy coloration? (which has a greater effect, or do they work equaly ro rnluence guppy populations?) open up a word document to begin your lab report - for this activity, the lab repoft includes only 3 sections (you can atso write this by hand) 1. Introduction - incrudes background information on guppies and your hypothesis 2. Data - a data tabre or graph that shows your guppy cororation over several generations xxyou can make a graph with microsoft exer, or you can go to an online graph-maker at **Make sure your data is defined - incrude beginning populations, number of generations, cororations and other pertinent labels 3. conclusions - answer the experimental question. The conclusion can be written in.about a paragraph. Be sure to inc.lude g;r"iir information about the trends in tne data to rrpport your conclusion.

3 -'- rn* simulation The simulation allows you to choose your starting population of guppies (coloration) and the number and types of predators in the habitat. Remember that you will need to compare 2 sets of data in order to make a clear determination about how predators and sex selection affect guppy populations. You will need 2 sets of data (and two graphs) to compare - in eftect run 2 simulations. You may want to run more than 2 simulations if you cannot make a determination with only 2. You will also need to make sure the simulation runs through enough generations to show patterns (5 minimal) Grading pt) Good (2 pts) Exellent (3 pts) Introduction Either background information or hypothesis is missing Background information included,could be expanded upon, hypothesis included Background information included and provides a context for the problem, hypothesis stated Data Conclusions Format Data is not included as a chart or graph; not enough data is taken to draw conclusions, difficu lt to interpret Conclusions do not follow data; experimental question is not answered; surnmary misslng or incoherent Organization Iacking; not neat (0 pts) Data includes enough information to draw conclusions, some labels are missing or data is difficult to interpret Conclusions appear to follow data, the experimental question is answered, summary of data missing or incoherent Neat, organized and easy to read (1 pt) Data is extensive and clearly shows trends in the populations. All graphs and charts are labeled and easy to read Conclusions loeically follow data, trends in data are summarized; the experimental question is answered and the hypothesis is addressed x 2 = Total of 2O possible

4 Sex and the Single Guppy The purpose of this activity is to analyze how guppy populations change over time. The simulation activity allows you to start with a pool of guppies and your choice of predators, you will be able to watch what happens to your guppy population and how the introduction of predators can affect the guppy's appearance. The simulation will help you understand what pressures drive guppy evolution. xactivity adapted from PBS - pbs,org ' :.--::;;,::, f..,: r,rr,:,.:,,,, :,::'.:.;;:] ',.',,.,..tt)::. :. -,'.-,"*i 1ii;ili*..r. I.'t, Open the Guppy Sex Slmulatorl!! http : / / www. pbs.o rg / wg bh / ev olutio n / sex / gu ppy/ ed-pop. html Introduction: 1. If being flashy and colorful attracts predators, why do you think guppies are so colorful? 2. After viewing the guppy gallery, pick the fish you find most interesting. What is the fish's scientific name, origin and average size? Describe the coloration of the fish you chose. 3. After viewing the predator gallery, pick the fish you find most interesting. What is the fish's common name, scientific name, and origin? 4. View the guppy's habitats, what habitat conditions would affect the predator populations? Endler's Discovery and Variations of Guppy's in Pools

5 5. Who is John Endler? What did he study and where did he study it? 6. For each of the three stream areas, describe the guppy coloration: Pool 1: Pool 2: Pool 3: 7. Develop your own hypothesis about guppy coloration. The hypothesis should answer the questions: Why do guppies in different areas of the stream have difference in coloration? (You can choose from the list on the simulation, or make up your own) Guppy Simulation % of Brightest Guppies ( 10 gerrerations) % of Bright GLrppies ( l0 generations) % of Drab GLrppies (10 generatious) o/o of DrabbestGLrppies ( 10 generations) Trial 1 Guppy: Even Mix Predators: l{) [(ivulus Trial2 Guppy: Even Mix Predators: l0 I{ivulus, J0 Acara Trial 3 Guppy: Even Mix Predators: J0 Rivulus, l0 Acara. l{) Cichlid Trial 4 Guppy: Mostly Bright Predators: -10 Rivulus Trial 5 Guppy: ivlostly Drab PredatOrS: _10 Il ivlrllrs. -l{),.\crra. -1t) ( iclrlitl

6 I summary B. Describe how predators influence guppy coloration. 9. Was your hypothesis correct, use your data to justify your answer. 10. What does it mean that"male guppies live in a crossfire between their enemies and their would be mates"? 11. Why do you think guppies in different areas of the stream have different coloration? 12. What would happen to mostly drab guppies that were placed in a stream with very few predators? 13. What would happen to brightly colored guppies that were placed in a stream with many predators?