S.C. Moldoveanu, A.G. Hudson, A. Harrison. R.J. Reynolds Tobacco Co.
|
|
- Agnes Young
- 5 years ago
- Views:
Transcription
1 The Determination of Diacetyl and Acetylpropionyl in Aerosols from Electronic Smoking Devices using Gas Chromatography Triple Quad Mass Spectrometry S.C. Moldoveanu, A.G. Hudson, A. Harrison R.J. Reynolds Tobacco Co.
2 Background 2,3-Butanedione (diacetyl) is used as a flavoring agent in food and it is a component of certain fragrances. Also the compound occurs naturally in certain alcoholic and non-alcoholic beverages and in several types of tobacco. Concerns have been raised about exposure to diacetyl, and it has been indicated that the compound may contribute or may even cause severe respiratory disorders in the workplace. Occupational Safety and Health Administration (OSHA) issued a guidance regarding the use of diacetyl as a food flavoring. National Institute for Occupational Safety and Health (NIOSH) also issued a number of reports regarding the exposure to diacetyl and 2,3-pentanedione (acetylpropionyl) in the working place. Analysis of diacetyl and acetylpropionyl in mainstream cigarette smoke (burndown products) and in aerosol (both the particles and the suspending gas) from electronic smoking devices (e-cigarettes) is of considerable interest.
3 Background (cont.) Measurements for diacetyl were performed on various matrices such as wine, beer, various types of food, urine, and air. The analysis of diacetyl in e-liquids and tobacco products has been previously reported and the procedures can be based on gas chromatography followed by mass spectrometry, or on HPLC. A CORESTA method for the analysis of aldehydes and ketones in smoke from cigarettes [1] has been modified to include the measurement of diacetyl and acetylpropionyl [2] at μg level. The evaluation of diacetyl and acetylpropionyl in e-vapor products has been previously presented at TSRC [3,4]. 1. CORESTA Recommended Method No March Cooperation Centre for Scientific Research Relative to Tobacco (CORESTA). 2. Pierce, J. S., A. Abelmann, L. J. Spicer, R. E. Adams, B. L. Finley: Crit. Rev. Toxicol, 44 (2014) Huang. C. B., K. Wagner, J. W. Flora, 69 th Tobacco Science Research Conference, Naples, Fl, Sept , 2015, Paper Fraley N., 70 th Tobacco Science Research Conference, Palm Beach Gardens, Fl, Sept , 2016, Poster 18.
4 Analytical method summary The aerosols from e-cigarettes were collected using a Cerulean SM 450 Linear Smoke Machine (Cerulean SM 450,Milton Keynes, UK) using a square wave profile. The collection from the e-cigarettes was performed on a Cambridge pad and one impinger containing 10 ml acetone. The e-liquids were obtained either directly from bottles or removed from cartridges by centrifugation. The analysis used a gas chromatographic separation on a Carbowax type column with the measurement of the analytes on a triplequad mass spectrometer (GC-MS/MS) working in positive MRM mode. The GC-MS/MS was 7890B-7000C from Agilent (Agilent Technologies Inc., Wilmington, DE, USA). The separation was performed on an Agilent J&W DB-WaxEtr chromatographic column, 30 m x 0.25 mm i.d. with 0.25 μm film.
5 Analytical method (details on sample collection) The smoking regimen for aerosol collection was 55 ml puff volume, 3 second puff duration, and 30 second puff interval. Depending on the electronic smoking device, 50 puffs or 100 puffs were collected at specific puff intervals such as 1-50, , etc. The impingers were weighed before and after sample collection to determine the remaining volume of acetone (some acetone evaporates during smoking). After the samples were collected, the particulate phase pads were placed in the impinger containing the corresponding vapor phase and extracted (to create combined whole aerosol samples). An aliquot of 1mL sample was taken and d 7 -quinoline is added. The collection of smoke from 3R4F Kentucky reference cigarette was performed under ISO or HCI protocols, using a Cambridge pad followed by two impingers each with 50 ml acetone and glass beads.
6 Publication of the method The present method has been published: S. C. Moldoveanu, A. G. Hudson, W. A. Scott, A. Harrison, The Determination of Diacetyl and Acetylpropionyl in Aerosols from Electronic Smoking Devices using Gas Chromatography Triple Quad Mass Spectrometry, Beitraege Tabak. Intern., 27(7) (2017)
7 Analytical method (details on GC separation) Parameter Description Parameter Description Initial oven temp. 35 C Inlet initial temp. 120 C Initial time 7.0 min Initial time 7 min Oven ramp rate 2.5 C/min Heating rate 600 o C/min Oven final first ramp 50 C Final inlet temp. 290 o C Final time first ramp 0 min Injection volume 3.0 ml Oven ramp rate 30 C/mm Carrier gas Helium Oven final second ramp 260 o C Flow mode Constant flow Final time 5.0 min Flow rate 1.1 ml/min Total run time 25 min Nominal initial pressure 12.2 psi Inlet type Multi Mode Average flow velocity cm/sec Inlet mode Pulse splitless Total flow 19.1 ml/min Pulse pressure 20 psi Holdup time 1.88 min Purge flow time 0.75 min Outlet pressure Vacuum Purge flow 15 ml/min Transfer line heater 280 C
8 Analytical method (details on MS/MS parameters) Parameter Description Ion source EI Acquisition mode MRM (positive) Source temperature 230 o C Collision cell He 2.25 ml/min N 2 collision gas 1.5 ml/min Gain factor 10 Diacetyl transition CE energy for diacetyl 2 V Acetylpropionyl transition CE energy for acetylpropionyl 1 V d 7 -Quinoline transition CE energy for d 7 -quinoline l 5 V MS1 resolution Unit MS2 resolution Unit Dwell time for each compound 150 ms
9 Typical chromatogram for a sample containing about 50 ng/ml of the analytes with I.S. at 50 ng/ml d 7 -quinoline
10 Analytical method (various details) Calibration standards in ng/ml. Standard Compound Level 7 Level 6 Level 5 Level 4 Level 3 Level 2 Level 1 Diacetyl Acetylpropionyl Coefficients for the calibration curves Y = a X 2 + b X + c used in quantitation, Y = ng/ml, X = (analyte area)/(i.s. area) Compound a b c R 2 Diacetyl Acetylpropionyl Internal standard (d 7 -quinolone) concentration in the analyzed samples was 50 ng/ml
11 Method validation Limit of detection (LOD) and limit of quantitation (LOQ) in ng/ml. Compound LOD (ng/ml) LOQ (ng/ml) Diacetyl Acetylpropionyl Limit of detection (LOD) and limit of quantitation (LOQ) in ng/puff for 50 puffs per pad extracted with 10 ml acetone. Compound LOD (ng/puff) LOQ (ng/puff) Diacetyl Acetylpropionyl Limit of detection (LOD) and limit of quantitation (LOQ) in ng/g e- liquid for 0.5 g e-liquid in 10 ml acetone. Compound LOD (ng/g) LOQ (ng/g) Diacetyl Acetylpropionyl
12 Method validation Relative standard deviation (RSD%) for standard Level 1 (1.05 ng/ml diacetyl and 1.23 ng/ml acetylpropionyl), and standard Level 3 (21.0 ng/ml diacetyl and 24.6 ng/ml acetylpropionyl) Compound Level 1 Level 3 Diacetyl 3.91% (n = 5) 4.89% (n = 10) Acetylpropionyl 1.48% (n = 5) 3.36% (n = 10)
13 Method validation (cont.) Results for the recovery study in ng/ml Sample Added diacetyl Diacetyl ng/ml Recovery % Added acetylpropionyl Acetylpropionyl ng/ml Recovery% E-Cig. X puffs RSD% E-Cig. X puffs RSD% E-Cig. X puffs RSD% E-Cig. X puffs RSD% E-Cig. X puffs RSD% E-Cig. X puffs RSD% Results in ng/puff for diacetyl and acetylpropionyl from two different smoking devices and two impinger collection. Electronic device/flavor Impinger 1 + Pad Diacetyl Impinger 2 Acetylpropionyl Impinger 1 + Pad Impinger 2 Device 1 ng/puff RSD% Device 2 ng/puff RSD%
14 Method validation (cont.) Results in μg/cigarette for diacetyl in 3R4F cigarettes. Smoking protocol Pad Impinger 1 Impinger 2 Total ISO Average μg/cig RSD% HCI Average μg/cig RSD% Results in μg/cigarette for acetylpropionyl in 3R4F cigarettes. Smoking protocol Pad Impinger 1 Impinger 2 Total ISO Average μg/cig RSD% HCI Average μg/cig RSD% Literature reference [1] indicated an average of μg/cig diacetyl and 15.4 μg/cig acetylpropionyl for ISO smoking. 1. Pierce, J. S., A. Abelmann, L. J. Spicer, R. E. Adams, B. L. Finley, Crit. Rev. Toxicol, 44 (2014) (pag. 422)
15 Results for aerosols from various smoking devices expressed in ng/puff Sample description Diacetyl Acetylpropionyl Brand Puff range ng/puff RSD% ng/puff RSD% E-cig. A E-cig. A E-cig. A E-cig. A E-cig. B E-cig. B E-cig. B E-cig. B E-cig. C E-cig. C E-cig. C E-cig. C E-cig. D E-cig. D E-cig. D E-cig. D
16 Results for aerosols from various smoking devices expressed in ng/puff (cont.) Sample description Diacetyl Acetylpropionyl Brand Puff range ng/puff RSD% ng/puff RSD% E-cig. E E-cig. E E-cig. F E-cig. F E-cig. G E-cig. G E-cig. H E-cig. H E-cig. I E-cig. J E-cig. K E-cig. K E-cig. L E-cig. L
17 Results for diacetyl in the aerosols from various smoking devices expressed in ng/puff
18 Results for acetylpropionyl in the aerosols from various smoking devices expressed in ng/puff
19 Results for various e-liquids expressed in ng/g Sample description Diacetyl Acetylpropionyl ng/g RSD% ng/g RSD% 1 E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid
20 Results for various e-liquids expressed in ng/g (cont.) Sample description Diacetyl Acetylpropionyl ng/g RSD% ng/g RSD% 19 E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid * E-liquid E-liquid E-liquid E-liquid E-liquid E-liquid * E-liquid * E-liquid * * 3 36 E-liquid * E-liquid * *Note: These values were outside the calibration range and were calculated based on a linear dependence Y (ng/ml) = X (for diacetyl) and Y = X (for acetylpropionyl).
21 Results for aerosols from various smoking devices expressed in ng/puff
22 Results for acetylpropionyl in various e-liquids expressed in ng/g
23 Conclusions A new method for the quantitation of diacetyl and acetylpropionyl in the aerosols of electronic smoking devices (ecigarettes) has been developed. The method also has been applied for the measurements of these two analytes in the liquids used in e-cigarettes (e-liquids). The method uses a GC-MS/MS instrument with separation on a Carbowax type column and detection in MRM positive mode. The method has been validated. The limit of quantitation (LOQ) for the method was determined to be 0.41 ng/ml for diacetyl and 0.21 ng/ml for acetylpropionyl as measured for standards. The accuracy of the method has been verified on a 3R4F Kentucky reference cigarette. The results for different e-cigarette brands and e-liquids indicated a wide range of levels of the two analytes.
Matt S. Melvin, Karen C. Avery, Jason W. Flora, Karl A. Wagner. Altria Client Services LLC l Regulatory Sciences 12Oct2016 Final 1
Determination of 2,3-Butanedione, 2,3-Pentanedione, and Acetoin in Electronic Cigarette Formulations and Aerosols by Gas Chromatography-Mass Spectrometry Matt S. Melvin, Karen C. Avery, Jason W. Flora,
More informationAnalysis of short chain organic acids in tobacco and in some flavored e-liquids
Analysis of short chain organic acids in tobacco and in some flavored e-liquids S.C. Moldoveanu, Karen B. Kilby, Amanda G. Hudson 1 Background Short chain organic acids from acetic to caprylic play an
More informationAnalysis of several common. organic acids in tobacco leaf, snus, and moist snuff
Analysis of several common organic acids in tobacco leaf, snus, and moist snuff S. C. Moldoveanu, W. A. Scott R.J. Reynolds Tobacco Co. Background Among the organic acids commonly present in tobacco, are
More informationRapid and Robust Detection of THC and Its Metabolites in Blood
Rapid and Robust Detection of THC and Its Metabolites in Blood Application Note Forensics/Doping Control Author Stephan Baumann Agilent Technologies, Inc. Santa Clara CA 95051 USA Abstract A robust method
More informationHighly Repeatable Ultra Low Detection of Estradiol Using Triple Quadrupole GC/MS in NCI Mode
Highly Repeatable Ultra Low Detection of Estradiol Using Triple Quadrupole GC/MS in NCI Mode Application Brief Biomedical Authors Melissa Churley Agilent Technologies, Inc. 5301 Stevens Creek Blvd Santa
More informationUptake and Metabolism of Phthalate Esters by Edible Plants
1 Supporting Information for 2 3 Uptake and Metabolism of Phthalate Esters by Edible Plants 4 Jianqiang Sun, Xiaoqin Wu, Jay Gan * 5 6 7 Department of Environmental Sciences, University of California,
More informationDetection of Cannabinoids in Oral Fluid with the Agilent 7010 GC-MS/MS System
Application Note Forensics, Workplace Drug Testing Detection of Cannabinoids in Oral Fluid with the Agilent 7010 GC-MS/MS System Authors Fred Feyerherm and Anthony Macherone Agilent Technologies, Inc.
More informationCORESTA Recommended Method No. 84
Cooperation Centre for Scientific Research Relative to Tobacco E-Vapour Sub-Group CORESTA Recommended Method No. 84 DETERMINATION OF GLYCERIN, PROPYLENE GLYCOL, WATER, AND NICOTINE IN THE AEROSOL OF E-CIGARETTES
More informationCORESTA Recommended Method No. 78
Cooperation Centre for Scientific Research Relative to Tobacco Smoke Analytes Sub-Group CORESTA Recommended Method No. 78 DETERMINATION OF SELECTED PHENOLIC COMPOUNDS IN MAINSTREAM CIGARETTE SMOKE BY HPLC-FLD
More informationChromatographic Profiling as a Tool in the Comparison and Evaluation of Complex Mixtures
Chromatographic Profiling as a Tool in the Comparison and Evaluation of Complex Mixtures James C. Rogers*, Lisa S. Winkler, and Michael F. Borgerding R.J. Reynolds Tobacco Company, Research and Development,
More informationForensic Analysis of Blood Alcohol Concentration
Application Note Forensics Forensic Analysis of Blood Alcohol Concentration Using the Agilent 886 GC with Agilent J&W DB BAC1 UI and Agilent J&W DB-BAC2 UI columns and the Agilent 7697A headspace sampler
More informationApplication. Detection of Cannabinoids in Oral Fluid Using Inert Source GC/MS. Introduction. Authors. Abstract. Forensic Toxicology
Detection of Cannabinoids in Oral Fluid Using Inert Source GC/MS Application Forensic Toxicology Authors Christine Moore, Sumandeep Rana, and Cynthia Coulter Immunalysis Corporation 829 Towne Center Drive
More informationMETHOD DEVELOPMENT AND VALIDATION: QUANTIFICATION OF METALS IN LIQUIDS AND AEROSOL OF E-CIGARETTES
METHOD DEVELOPMENT AND VALIDATION: QUANTIFICATION OF METALS IN LIQUIDS AND AEROSOL OF E-CIGARETTES Sandra Otte, Sonja Nowak & Michael Intorp Imperial Tobacco Group; Special Analytes Laboratory Hamburg,
More informationA New Method for the Analysis of Carbonyl Compounds in e-cigarette Liquids
A New Method for the Analysis of Carbonyl Compounds in e-cigarette Liquids Alex Martin Fraser Williamson CORESTA Congress, Québec, Canada October 16, 2014 www.aristalabs.com Outline Introduction Analytical
More informationDetermination of red blood cell fatty acid profiles in clinical research
Application Note Clinical Research Determination of red blood cell fatty acid profiles in clinical research Chemical ionization gas chromatography tandem mass spectrometry Authors Yvonne Schober 1, Hans
More informationParticle Size Distribution of E-Cigarette Aerosols and the Relationship to Cambridge Filter Pad Collection Efficiency
Particle Size Distribution of E-Cigarette Aerosols and the Relationship to Cambridge Filter Pad Collection Efficiency S.L Alderman, C. Song, S. Moldoveanu, S.K. Cole R.J. Reynolds Tobacco Company, Winston-Salem,
More informationAnalysis of (S)- and (R)-nicotine
Analysis of (S)- and (R)-nicotine in various nicotine samples and in e-liquids S. C. Moldoveanu*, G. M. Dull**, R. J. Oden*, W. A. Scott* *R.J. Reynolds Tobacco Co. **RAI Innovation Co. Background Nicotine
More informationScreening by immunoassay and confirmation & quantitation by GC-MS of buprenorphine and norbuprenorphine in urine, whole blood and serum
Screening by immunoassay and confirmation & quantitation by GC-MS of buprenorphine and norbuprenorphine in urine, whole blood and serum NINA KANGAS, SIRPA MYKKÄNEN, SANNA KYLLÖNEN, PÄIVI RAJALA, KARI ARINIEMI
More informationExtraction of 11-nor-9-carboxy-tetrahydrocannabinol from Hydrolyzed Urine by ISOLUTE. SLE+ Prior to GC/MS Analysis
Application Note AN84 Extraction of -nor-9-carboxy-tetrahydrocannabinol from Hydrolyzed Urine by ISOLUTE SLE+ Page Extraction of -nor-9-carboxy-tetrahydrocannabinol from Hydrolyzed Urine by ISOLUTE SLE+
More informationExtraction of Multiple Mycotoxins From Nuts Using ISOLUTE Myco prior to LC-MS/MS Analysis
Application Note AN784 Extraction of Multiple Mycotoxins from Nuts Using ISOLUTE Myco Page 1 Extraction of Multiple Mycotoxins From Nuts Using ISOLUTE Myco prior to LC-MS/MS Analysis This application note
More informationCORESTA RECOMMENDED METHOD NÄ 9
CORESTA RECOMMENDED METHOD NÄ 9 DETERMINATION OF NICOTINE IN CIGARETTE FILTERS BY GAS CHROMATOGRAPHIC ANALYSIS (April 2009) 0. INTRODUCTION In 2001 the CORESTA Routine Analytical Chemistry Sub-Group was
More informationToxicology Screening of Whole Blood Extracts Using GC/Triple Quadrupole/MS
Toxicology Screening of Whole Blood Extracts Using GC/Triple Quadrupole/MS Application Note Forensic Toxicology Authors Bruce Quimby and Mike Szelewski Agilent Technologies, Inc. 2850 Centerville Road
More informationSecrets to Successful GC-MS/MS Pesticide Method Development
Secrets to Successful GC-MS/MS Pesticide Method Development Katerina Mastovska Nutritional Chemistry and Food Safety Covance Laboratories QuEChERS vs. Traditional Methods Quick, Easy, Cheap, Effective,
More informationAnalysis of anti-epileptic drugs in human serum using an Agilent Ultivo LC/TQ
Application Note Clinical Research Analysis of anti-epileptic drugs in human serum using an Agilent Ultivo LC/TQ Authors Jennifer Hitchcock 1, Lauren Frick 2, Peter Stone 1, and Vaughn Miller 2 1 Agilent
More informationA FORENSIC TOXICOLOGY METHOD FOR THE DETERMINATION OF DESOMORPHINE, HEROIN, METHADONE, BUPRENORPHINE AND METABOLITES IN URINE USING LC/MS QQQ
FORENSICS MARKET A FORENSIC TOXICOLOGY METHOD FOR THE DETERMINATION OF DESOMORPHINE, HEROIN, METHADONE, BUPRENORPHINE AND METABOLITES IN URINE USING LC/MS QQQ Desomorphine, also known by its street name
More informationExtraction of Multiple Mycotoxins From Grain Using ISOLUTE Myco prior to LC-MS/MS Analysis
Application Note AN782 Extraction of Multiple Mycotoxins from Grain Using ISOLUTE Myco Page 1 Extraction of Multiple Mycotoxins From Grain Using ISOLUTE Myco prior to LC-MS/MS Analysis This application
More informationRapid Analysis of 37 FAMEs with the Agilent 8860 Gas Chromatograph
Application Note Food Rapid Analysis of 37 FAMEs with the Agilent 88 Gas Chromatograph Author Youjuan Zhang Agilent Technologies (Shanghai) Co. Ltd., Shanghai 131 P. R. China Abstract An Agilent 88 GC
More informationDevelopment and Validation of Multiresidue Pesticide Methods at FDA/CFSAN
Development and Validation of Multiresidue Pesticide Methods at FDA/CFSAN Jon W. Wong U.S. Food and Drug Administration Center for Food Safety and Applied Nutrition American Council of Independent Laboratories
More informationApplicant Name: GD Sigelei Electroinc Tech Co., Ltd B7 Building, No.1 District, Xicheng Science and Technology Park, Hengli Town, Dongguan, China
Date: Jan.17, 2018 Applicant Name: GD Sigelei Electroinc Tech Co., Ltd Applicant Add.: B7 Building, No.1 District, Xicheng Science and Technology Park, Hengli Town, Dongguan, China Test sample(s) was(were)
More informationRapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases
Clinical, Forensic & Toxicology Applications Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases By Shun-Hsin
More informationDetermination of Amantadine Residues in Chicken by LCMS-8040
Liquid Chromatography Mass Spectrometry Determination of Amantadine Residues in Chicken by LCMS-8040 A method for the determination of amantadine in chicken was established using Shimadzu Triple Quadrupole
More informationSPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine
SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 709 Key Words SPE, SOLA
More informationGas Chromatography/Mass Spectrometry Approaches to the Analysis of Acrylamide in Foods Application
Gas Chromatography/Mass Spectrometry Approaches to the Analysis of Acrylamide in Foods Application Food Safety Author Bernhard Rothweiler Agilent Technologies Deutschland Gmb ewlett-packard Strasse 8 76337
More informationPHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES. DR. A. RAMESH, Ph.D, D.Sc.,
PHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES DR. A. RAMESH, Ph.D, D.Sc., raamesh_a@yahoo.co.in 1 OBJECTIVES Determination of persistence and photolysis
More informationRobust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS
Application Note 242 Robust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS INTRODUCTION Tobacco-specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco products. They
More informationAmphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 6430 LC/MS/MS
Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 643 LC/MS/MS Application Note Forensics Authors Jason Hudson, Ph.D., James Hutchings, Ph.D., and Rebecca Wagner, Ph.D. Virginia
More informationAnalysis of Acrylamide in French Fries using Agilent Bond Elut QuEChERS AOAC kit and LC/MS/MS
Analysis of Acrylamide in French Fries using Agilent Bond Elut QuEChERS AOAC kit and LC/MS/MS Food Application Author Fadwa Al-Taher Institute for Food Safety and Health Illinois Institute of Technology
More informationS4. Summary of the GALNS assay validation. Intra-assay variation (within-run precision)
S4. Summary of the GALNS assay validation (i.) Intra-assay variation (within-run precision) Intra-assay variation was determined by measuring standard blood samples (low activity standard; medium activity
More information[application note] Simultaneous detection and quantification of D 9 THC, 11-OH-D 9 T H C and D 9 THC-COOH in whole blood by GC tandem quadrupole MS
Simultaneous detection and quantification of D 9 THC, 11-OH-D 9 T H C and D 9 THC-COOH in whole blood by GC tandem quadrupole MS Marie Bresson, Vincent Cirimele, Pascal Kintz, Marion Villain; Laboratoire
More informationDetermination of Bath Salts (Pyrovalerone Analogs) in Biological Samples
Determination of Bath Salts (Pyrovalerone Analogs) in Biological Samples Application Note Forensic Toxicology Authors Joe Crifasi Saint Louis University Forensic Toxicology Laboratory Saint Louis, Mo.
More informationDetermination of Benzodiazepines in Urine by CE-MS/MS
Determination of Benzodiazepines in Urine by CE-MS/MS Application ote Forensic Toxicology Authors audimir Lucio do Lago Department of Fundamental Chemistry, Institute of Chemistry University of São Paulo,
More informationDetermination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No.
Page 1 of 10 Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection EPL-BAS Method No. 205G881B Method Summary: Residues of 6-CPA are
More informationLC/MS/MS of Trichothecenes and Zearalenone in Wheat Using Different Sample Prep Methods
LC/MS/MS of Trichothecenes and Zearalenone in Wheat Using Different Sample Prep Methods Application Note Food Testing & Agriculture Authors Nick Byrd and Danielle Sweeney Campden BRI Gloucestershire UK
More informationDETERMINATION OF CANNABINOIDS, THC AND THC-COOH, IN ORAL FLUID USING AN AGILENT 6490 TRIPLE QUADRUPOLE LC/MS
FORENSICS AND TOXICOLOGY ANALYSIS DETERMINATION OF CANNABINOIDS, THC AND THC-COOH, IN ORAL FLUID USING AN AGILENT 6490 TRIPLE QUADRUPOLE LC/MS Solutions for Your Analytical Business Markets and Applications
More informationGC-MS/MS Analysis of Benzodiazepines Using Analyte Protectants
GC-MS/MS Analysis of Benzodiazepines Using Analyte Protectants Jeremy Matthews, 1 Alex Chen, 2 and Flavio Bedini 1 1 Thermo Fisher Scientific, Singapore; 2 Alpha Analytical Pte Ltd, Singapore Overview
More informationA Robustness Study for the Agilent 6470 LC-MS/MS Mass Spectrometer
A Robustness Study for the Agilent 7 LC-MS/MS Mass Spectrometer Application Note Clinical Research Authors Linda Côté, Siji Joseph, Sreelakshmy Menon, and Kevin McCann Agilent Technologies, Inc. Abstract
More informationMaria João Mendes Mendonça Barreira Department of Health Promotion and Chronic Deseases, INSA
Quantification of four immunosuppressant drugs by Liquid Chomatography-Tandem Mass Spectrometry using Direct Injection. Maria João Mendes Mendonça Barreira Department of Health Promotion and Chronic Deseases,
More informationusing the Agilent 7696A Sample Prep
Automated Clean-up for Mineral Oil (Hydrocarbon Oil Index) Analysis using the Agilent 7696A Sample Prep WorkBench Application Note Automated Sample Preparation Authors Frank David, Karine Jacq, and Bart
More informationFatty Acid Mass Spectrometry Protocol Updated 10/11/2007 By Daren Stephens
Fatty Acid Mass Spectrometry Protocol Updated 10/11/2007 By Daren Stephens Synopsis: This protocol describes the standard method for extracting and quantifying free fatty acids found in cells and media
More informationApplication Note. Author. Abstract. Introduction. Food Safety
Determination of β2-agonists in Pork with SPE eanup and LC-MS/MS Detection Using Agilent BondElut PCX Solid-Phase Extraction Cartridges, Agilent Poroshell 120 column and Liquid Chromatography-Tandem Mass
More informationDetermination of Gamma-Hydroxy-Butyrate (GHB) in Biological Samples
Determination of Gamma-Hydroxy-Butyrate (GHB) in Biological Samples Application Note Forensic Toxicology Authors Joe Crifasi Saint Louis University Forensic Toxicology Laboratory Saint Louis, MO, USA Ron
More informationDetermination of N-Nitrososarcosine (NSAR) in tobacco
JTI-Ökolab Vienna, Austria Determination of N-Nitrososarcosine (NSAR) in tobacco Madeleine Werneth, Jutta Pani, Bernhard Mayer-Helm 2014 CORESTA CONGRESS - ST46 Québec City, Canada 12-16 October 2014 Background
More informationCOMPARISON OF RESULTS FROM THREE TECHNIQUES FOR THE ESTIMATION OF E-LIQUID ph-values
COMPARISON OF RESULTS FROM THREE TECHNIQUES FOR THE ESTIMATION OF E-LIQUID ph-values J.H. Lauterbach, Ph.D., DABT Lauterbach & Associates, LLC Macon, GA 31210-4708 Quotations from Pagano et al., 2016 E-Cigs
More informationDetermination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry
Determination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry Application Note Food Safety Authors Chenhao Zhai Agilent
More informationQuantitative Analysis of -Hydroxybutyrate in Hair Using Target Analyte Finding Processing of Comprehensive GC-HRT Data
Quantitative Analysis of -Hydroxybutyrate in Hair Using Target Analyte Finding Processing of Comprehensive GC-HRT Data LECO Corporation; Saint Joseph, Michigan USA Key Words: Pegasus GC-HRT, GHB, Hair,
More informationDetermination of Residues of 1,3-Dichloropropene in Grapes by Capillary Gas Chromatography with Mass Selective Detection
Dow AgroSciences LLC 9330 Zionsville Road Indianapolis, Indiana 46268-1054 GRM: 99.09.R1 EFFECTIVE: May 26, 2000 SUPERSEDES: 99.09 Determination of Residues of 1,3-Dichloropropene in Grapes by Capillary
More informationLC-MS/MS Method for the Determination of Tenofovir from Plasma
LC-MS/MS Method for the Determination of Tenofovir from Plasma Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 687 Key Words SPE, SOLA CX, Hypersil GOLD, tenofovir Abstract
More informationScreening of Antihistamine Agents (Diphenhydramine) with Blood and Urine Samples by REMEDi-HS System
Screening of Antihistamine Agents (Diphenhydramine) with Blood and Urine Samples by REMEDi-HS System Ohtsuji M, Ohshima T, Takayasu T, Nishigami J, Kondo T, Lin Z, Minamino T Department of Legal Medicine,
More informationAll stocks and calibration levels were prepared in water: methanol (50:50) v/v to cover range of all steroid concentrations (refer Table 1).
Application LCMS-8040 Simultaneous determination of 11 steroids and Vitamin D2/D3 in human serum using LC/MS/MS - Introduction Quantification of endogenous hormonal steroids and their precursors is essential
More informationRobust extraction, separation, and quantitation of structural isomer steroids from human plasma by SPE-UHPLC-MS/MS
TECHNICAL NOTE 21882 Robust extraction, separation, and quantitation of structural isomer steroids human plasma by SPE-UHPLC-MS/MS Authors Jon Bardsley 1, Kean Woodmansey 1, and Stacy Tremintin 2 1 Thermo
More informationSCOPE OF ACCREDITATION TO ISO/IEC 17025:2017
SCOPE OF ACCREDITATION TO ISO/IEC 17025:2017 R. J. REYNOLDS TOBACCO COMPANY 1 Product Services Analytical Testing Laboratories 950 Reynolds Blvd. Winston Salem, NC 27102 Jannell Rowe Phone: 336 741 4121
More informationA Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS
A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS By Shun-Hsin Liang and Frances Carroll Abstract Vitamin K₁ and K₂ analysis is typically complex and time-consuming because these
More informationModified QuEChERS Procedure for Analysis of Bisphenol A in Canned Food Products
Modified QuEChERS Procedure for Analysis of Bisphenol A in Canned Food Products UCT Product Number: ECQUEU75CT-MP (pouch contains 4 mg MgSO 4, 1 mg NaCl, 5 mg Na citrate dibasic sesquihydrate, 1 mg Na
More informationHigh-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring Buprenorphine and Norbuprenorphine in Urine
High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring and in Urine Xiaolei Xie, Joe DiBussolo, Marta Kozak; Thermo Fisher Scientific, San Jose, CA Application Note 627 Key Words, norbuprenorphine,
More information2017_ST10_JinCathy.pdf. SSPT Document not peer-reviewed by CORESTA
Investigation of TSNA Formation in Electronic Cigarette Liquids and Aerosols Xiaohong Cathy Jin, Karen C. Avery, Georgios D. Karles, William P. Gardner, Matt S. Melvin, Donna C. Smith, Willie J. Mckinney,
More informationAbstract. Introduction
A Fast Dilute-And-Shoot Method for Simultaneous 5-Hydroxyindoleacetic Acid (5-HIAA), Vanillylmandelic Acid (VMA), and Homovanillic Acid (HVA) LC-MS/MS Analysis in Human Urine By Shun-Hsin Liang and Sue
More informationTechnical Report. E-Liquid Preliminary Proficiency Study
E-Cigarette Task Force Technical Report E-Liquid Preliminary Proficiency Study March 2015 Author: Rana Tayyarah Lorillard Tobacco Company Task Force Coordinator: Chuck Garner, Ph.D. R. J. Reynolds Tobacco
More informationMALAYSIAN MALAYSIAN COCOA WORKSHOP ON THE SAFE USE OF PESTICIDES IN COCOA AND HARMONIZED
COCOA WORKSHOP ON THE SAFE USE OF PESTICIDES IN COCOA AND HARMONIZED Pesticides - Commonly used in cocoa production Residue problems - health hazard GAP & Legislation CODEX Alimentarius - introduced and
More informationThe Effect of Puff Profile and Volume on the Yields of E-Cigarettes
The Effect of Puff Profile and Volume on the Yields of E-Cigarettes Dr M J Taylor, Filtrona Technology Centre Smoke Science/Product Technology Meeting Seville, Spain, 29 September to 03 October 2013 Introduction
More informationThe Effect of Puff Duration on Smoke Chemistry
The Effect of Puff Duration on Smoke Chemistry Eun-Jung Han* John Lee, Hae-Geun Shin, Ga-Hee Lim, Hyo-Keun Kim Oct 15, 2014 Contents 1 Introduction 2 Experimental 3 Results & Discussion 4 Conclusion 2
More informationAnalysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research
Analysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research Dominic Foley, Michelle Wills, and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION
More informationCannabinoid Quantitation Using an Agilent 6430 LC/MS/MS
Cannabinoid Quantitation Using an Agilent 643 LC/MS/MS Application Note Forensics Authors Jason Hudson, Ph.D., James Hutchings, Ph.D., and Rebecca Wagner, Ph.D. Virginia Department of Forensic Science
More informationLC-MS/MS Method for the Determination of 21 Opiates and Opiate Derivatives in Urine
LC-MS/MS Method for the Determination of 21 Opiates and Opiate Derivatives in Urine J. Jones, S. Westwood, T. Liddicoat, L. Pereira, T. Edge Thermo Fisher Scientific, Manor Park, Runcorn, UK Overview Purpose:
More informationVitamin D3 and related compounds by ESI and APCI
Liquid Chromatography Mass Spectrometry SSI-LCMS-9 Vitamin D and related compounds by ESI and APCI LCMS-8 Summary Vitamin D and related compounds were measured by LC-ESI/APCI-MS-MS. Background Accurate
More informationDEVELOPMENT OF A TECHNIQUE FOR THE DETERMINATION OF ph-values OF AEROSOLS GENERATED BY E-CIGARETTES
DEVELOPMENT OF A TECHNIQUE FOR THE DETERMINATION OF ph-values OF AEROSOLS GENERATED BY E-CIGARETTES John H. Lauterbach, Ph.D., DABT Lauterbach & Associates, LLC Macon, GA 31210-4708 USA Outline for presentation
More informationAgilent 6490 Triple Quadrupole LC/MS System with ifunnel Technology. Ultra sensitive quantitative performance
Agilent 6490 Triple Quadrupole LC/MS System with ifunnel Technology Ultra sensitive quantitative performance Unmatched quantitative performance for the most challenging analyses The Agilent 6490 Triple
More informationE-Vapour (EVAP) Sub-Group 2017 Report. Kitzbühel, Austria October 10, 2017
E-Vapour (EVAP) Sub-Group 2017 Report Kitzbühel, Austria October 10, 2017 History Evolution o 2014, formation of E-cigarette TF o 2016, TF activities completed; formation of E-vapor product SG 2 E-Vapour
More informationNeosolaniol. [Methods listed in the Feed Analysis Standards]
Neosolaniol [Methods listed in the Feed Analysis Standards] 1 Simultaneous analysis of mycotoxins by liquid chromatography/ tandem mass spectrometry [Feed Analysis Standards, Chapter 5, Section 1 9.1 ]
More informationApplication Note LCMS-108 Quantitation of benzodiazepines and Z-drugs in serum with the EVOQ TM LC triple quadrupole mass spectrometer
Application Note LCMS-108 Quantitation of benzodiazepines and Z-drugs in serum with the EVOQ TM LC triple quadrupole mass spectrometer Abstract This study demonstrates a sensitive, rapid and reliable research
More informationExtraction of Aflatoxin M1 From Infant Formula Using ISOLUTE Myco SPE Columns prior to LC-MS/MS Analysis
Application Note AN807 Extraction of Aflatoxin M From Infant Formula Using ISLUTE Myco Page Extraction of Aflatoxin M From Infant Formula Using ISLUTE Myco SPE Columns prior to LC-MS/MS Analysis This application
More informationFast and simultaneous analysis of ethanol metabolites and barbiturates using the QTRAP 4500 LC-MS/MS system
Fast and simultaneous analysis of ethanol metabolites and barbiturates using the QTRAP 4500 LC-MS/MS system Xiang He 1, Adrian Taylor 2 and Alexandre Wang 1 1 SCIEX, Redwood City, USA. 2 SCIEX, Concord,
More informationValidation Report 23 B
EURL for Cereals and Feeding stuff National Food Institute Technical University of Denmark Validation Report 23 B Determination of pesticide residues in wheat, rye, oat and rice by LC-MS/MS and GC-MS/MS
More informationLC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell 120 EC C18 Column
Application Note Clinical Research LC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell EC C8 Column Authors Rongjie Fu and Zhiming Zhang Agilent Technologies
More informationQuantitation of Protein Phosphorylation Using Multiple Reaction Monitoring
Quantitation of Protein Phosphorylation Using Multiple Reaction Monitoring Application Note Authors Ning Tang, Christine A. Miller and Keith Waddell Agilent Technologies, Inc. Santa Clara, CA USA This
More informationDetection of Low Level of Chloramphenicol in Milk and Honey with MIP SPE and LC-MS-MS
Detection of Low Level of Chloramphenicol in Milk and Honey with MIP SPE and LC-MS-MS Olga Shimelis, An Trinh, and Michael Ye Supelco, Div. of Sigma-Aldrich, Bellefonte, PA T407125 Introduction Molecularly
More informationOrganophosphorus Pesticides Analysis Using an Agilent J&W DB-5ms Ultra Inert Capillary GC Column. Application Note. Environmental. Abstract.
Organophosphorus Pesticides Analysis Using an Agilent J&W DB-5ms Ultra Inert Capillary GC Column Application Note Environmental Authors Doris Smith and Kenneth Lynam Agilent Technologies, Inc. 2850 Centerville
More informationDetermination of Amphetamine and Derivatives in Urine
Determination of Amphetamine and Derivatives in Urine Using a Modified QuEChERS and Capillary Electrophoresis Tandem Mass Spectrometry Analysis Application Note Authors Vagner B. dos Santos and Claudimir
More information4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column
Clinical, Forensic & Toxicology Applications 4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column By Amanda Rigdon Abstract A rapid, sensitive method
More informationCORESTA Recommended Method No. 74
Cooperation Centre for Scientific Research Relative to Tobacco Smoke Analytes Sub-Group CORESTA Recommended Method No. 74 DETERMINATION OF SELECTED CARBONYLS IN MAINSTREAM CIGARETTE SMOKE BY HPLC February
More informationSupporting Information
Supporting Information Development of a High Coverage Pseudotargeted Lipidomics Method Based on Ultra-High Performance Liquid Chromatography-Mass Spectrometry Qiuhui Xuan 1,2#, Chunxiu Hu 1#, Di Yu 1,2,
More informationDetermination of Allergens in Fragrance Products Using Agilent Deconvolution Reporting Software Application Brief
Determination of Allergens in Fragrance Products Using Agilent Deconvolution Reporting Software Application Brief Wei Luan, Chris Sandy, and Mike Szelewski Flavor and Fragrance The 7th amendment of the
More informationPossibility of Use a Saliva for Determination Ethanol and Opiates
Possibility of Use a Saliva for Determination Ethanol and Opiates Wojciech Piekoszewski 1,2, Wojciech Gubała 1, Ewa Janowska 1, Janusz Pach 3, Dariusz Zuba 1 1 Institute of Forensic Research, Westerplatte
More informationAnalysis and Quantitation of Cocaine on Currency Using GC-MS/MS. No. GCMS No. SSI-GCMS-1501
Gas Chromatograph Mass Spectrometer No. GCMS-1501 Analysis and Quantitation of Cocaine on Currency Using GC-MS/MS Shilpi Chopra, Ph.D., Laura Chambers Introduction Cocaine (CAS # 50-36-2), a white crystalline
More informationE-Cigarette Aerosol Analysis Report
Report No. : TCT7226C0 Date : Jan. 04, 208 Page No.: of 9 Applicant: Address: ALD GROUP LIMITED ALD Building NO.2 Industrial 3rd Road, ShiXin Community, Shiyan Town, Bao An District, Shenzhen 5808, China.
More informationQuantification of lovastatin in human plasma by LC/ESI/MS/MS using the Agilent 6410 Triple Quadrupole LC/MS system
Quantification of lovastatin in human plasma by LC/ESI/MS/MS using the Agilent 641 Triple Quadrupole LC/MS system Application Note Clinical Research Author Siji Joseph Agilent Technologies Bangalore, India
More informationUPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes
UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F
More informationEU Priority PAH Analysis in Pumpkin Seed Oil Using Bond Elut EMR Lipid Cleanup by GC/MS/MS
Application Note Food Testing & Agriculture EU Priority PAH Analysis in Pumpkin Seed Oil Using Bond Elut EMR Lipid Cleanup by GC/MS/MS Authors Thorsten Bernsmann Chemisches und Veterinäruntersuchungsamt
More informationCannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system
Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D
More informationCigarettes Determination of tobacco specific nitrosamines in mainstream cigarette smoke Method using LC-MS/MS
INTERNATIONAL STANDARD ISO 19290 First edition 2016-12-01 Cigarettes Determination of tobacco specific nitrosamines in mainstream cigarette smoke Method using LC-MS/MS Cigarettes Dosage des nitrosamines
More information