Forensic Drug Detection. ELISA Test Kits and Reagents Oral Fluid Collection System Automated Equipment. Simple Sensitive Solutions
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1 Forensic Drug Detection ELISA Test Kits and Reagents Oral Fluid Collection System Automated Equipment Simple Sensitive Solutions
2 Your Complete Forensic Drug Detection Source Neogen Corporation develops, manufactures, and markets a diverse line of products used to provide solutions for drug detection, and food and animal safety. Founded in 1982, Neogen (NASDAQ: NEOG) has grown to more than 1,000 employees with multiple U.S. and international locations, and a worldwide presence. Neogen s more than 30 years spent developing and manufacturing enzymelinked immunosorbent assay (ELISA) test kits has produced the highest quality, most dependable reagents with excellent reproducibility and sensitivity. Our comprehensive line of drug detection assays includes more than 115 ELISA kits that can be used to screen more than 300 drugs and/or their metabolites in a wide range of forensic matrices. Our test kits are qualitative one-step kits intended as screening devices for the detection of drugs and/ or their metabolites in forensic samples only. Suspect samples should be confirmed by a quantitative method, such as GC/MS. Our easy-to-use protocols are designed for high and low volume laboratories as they can be run manually or easily automated on a wide range of immunoassay instruments. Neogen s experienced technical support staff is available to discuss your testing and automation requirements to find a system that will work best for your applications. With our experienced research and development teams, and flexible operations, Neogen can quickly respond to rapidly evolving needs in the forensic market. Neogen believes that maintaining earned trust is a combination of innovation and a continuous commitment to quality. Neogen s quality management system is ISO 9001 certified, which communicates to the company s worldwide customer base that it understands the quality standards customers expect, and is firmly committed to achieving those quality standards. Neogen s continuing record of exceptional performance has earned it increasing recognition, including being repeatedly named as one of Forbes Magazine s Best Small Companies in America; being named to Fortune Magazine s prestigious list of 40 Stocks To Retire On; and being selected to NASDAQ s top tier of companies, the NASDAQ Global Select Market. Neogen s drug detection product line offers: More than 100 drug detection test kits Validation for forensic matrices: whole blood, urine, hair, oral fluid, serum, plasma, meconium, breast milk and others High sensitivity Excellent reproducibility Automated and semi-automated equipment packages Reagent Rental Program Customized single-line reporting software More than 30 years of ELISA experience Neogen offers products for multiple drug panels: Stimulants Anabolic Steroids/Beta-Agonists Hallucinogens and Anesthetics Antidepressants/Sedatives Narcotic Analgesics Anti-Inflammatories Antipsychotics Other Prescription Drugs Workplace Testing Designer Drugs 2 To order, call: (USA/Canada) or Fax: inform@neogen.com Web:
3 Drug Detection ELISA Panels Stimulants Amphetamine Amphetamine Specific Amphetamine Ultra Benzylpiperazine (BZP) Caffeine/Pentoxifylline Cocaine/BZE Cotinine Doxapram Mazindol/Mazindol Metabolites Methylphenidate Methamphetamine/MDMA Nikethamide Synthetic Cathinones (Methcathinone) Anabolic Steroids/Beta-Agonists Anabolic Steroids 16ß-Hydroxystanozolol Boldenone Nandrolone Stanozolol Trenbolone Beta-Agonists Bronchodilator Group Clenbuterol Ractopamine Theophylline Hallucinogens & Anesthetics Dextromethorphan Ketamine Lidocaine LSD Mepivacaine PCP Procaine THC Synthetic Cannabinoids Antidepressants/Sedatives Azaperone Barbiturate Group Benzodiazepine Group (Oxazepam/Clonazepam) Buspirone Citalopram Detomidine Flunitrazepam Fluoxetine Hydroxyzine Reserpine Sertraline Trazodone Tricyclics Group Zolpidem Narcotic Analgesics Alfentanil Anileridine Buprenorphine Buprenorphine/Norbuprenorphine Butorphanol Carfentanil Diprenorphine Etorphine Fentanyl Hydromorphone Levallorphan Lofentanil Meperidine Enhanced Methadone Methadone/LAAM Naltrexone/Nalbuphine Opiates Oxycodone/Oxymorphone Pentazocine Propoxyphene Sufentanil Tramadol Anti-Inflammatories Carprofen Celecoxib Corticosteroid Group Dexamethasone Flunixin Ibuprofen Ketorolac Methylprednisolone Phenylbutazone Salicylates Triamcinolone Acetonide Zomepirac Antipsychotics Droperidol Fluphenazine Haloperidol Haloperidol Metabolites Promazine Group Tricyclics Group FOR FORENSIC USE ONLY Other Prescription Drugs Amphetamine Amphetamine Specific Amphetamine Ultra Barbiturate Group Benzodiazepine Group (Oxazepam/Clonazepam) Buprenorphine Buprenorphine/Norbuprenorphine Butorphanol Carisoprodol Fentanyl Hydrocodone Hydrochlorothiazide Hydromorphone Ketamine Meperidine Enhanced Methadone Methadone/LAAM Methamphetamine/MDMA Methylphenidate Naltrexone/Nalbuphine Opiate Group Oxycodone/Oxymorphone Pentazocine Phenytoin Propoxyphene Tramadol Trazodone Zolpidem Workplace Testing Amphetamine Amphetamine Specific Amphetamine Ultra Barbiturate Group Benzodiazepine Group Buprenorphine Cocaine/BZE Methadone Methamphetamine/MDMA Opiates Oxycodone/Oxymorphone PCP Propoxyphene THC Designer Drugs Synthetic Cannabinoids (JWH-018) Synthetic Cannabinoids (JWH-250) Synthetic Cannabinoids (UR-144/XLR-11) Synthetic Cathinones (Methcathinone) Designer Benzodiazepines To order, call: (USA/Canada) or Fax: Web: 3
4 Drug Detection ELISA Kits ELISA Test Kits Product No. ELISA Test Kits Product No. ELISA Test Kits Product No. 16β-Hydroxystanozolol Acetaminophen Alfentanil Amphetamine Amphetamine Specific Amphetamine Ultra Anileridine Apomorphine/Apocodeine Azaperone Barbiturate Group Benzodiazepine Group (Oxazepam/Clonazepam) Benzylpiperazine Boldenone Bronchodilator Group Bumetanide Buprenorphine Buspirone Butorphanol Caffeine/Pentoxifylline Carfentanil Carisoprodol Carprofen Celecoxib Citalopram Clenbuterol Clonidine/Romifidine Cocaine/Benzoylecgonine Corticosteroid Group Cotinine (Serum) CT-96-S Cotinine (Urine) CT-96-U Creatinine Cromoglycate Dantrolene Detomidine Dexamethasone Dextromethorphan Diprenorphine Doxapram Droperidol Ethacrynic Acid Etorphine Fenspiride Fentanil Group Fentanyl Fexofenadine/Terfenadine Flunitrazepam Flunixin Fluoxetine Fluphenazine Furosemide Glycopyrrolate Guanabenz Haloperidol Haloperidol Metabolites Hydrochlorothiazide Hydromorphone Hydroxyzine Ibuprofen Ipratropium/Atropine Isoxsuprine Ketamine Ketoprofen Ketorolac Levallorphan Lidocaine Lofentanil LSD Mazindol/Mazindol Metabolites Meperidine Enhanced Mephentermine Mepivacaine Meprobamate Metaraminol Methadone Methadone/LAAM Methamphetamine/MDMA Methocarbamol Methotrexate Methylphenidate Methylprednisolone Naltrexone/Nalbuphine Nandrolone Nikethamide Opiate Group Oxycodone/Oxymorphone Pentazocine Phencyclidine (PCP) Phenylbutazone Phenytoin Procaine Promazine Group Propoxyphene Propranolol Pyrilamine Ractopamine Reserpine Salicylates Sertraline Stanozolol Sufentanil Sulfamethazine Neogen s Ready-to-Use (RTU) Forensic Assays Synthetic Cannabinoids (JWH-018) Synthetic Cannabinoids (JWH-250) Synthetic Cannabinoids (UR-144/XLR-11) Synthetic Cathinones (Methcathinone) THC Theophylline Tramadol Trazodone Trenbolone Triamcinolone Acetonide Tricyclics Group Zolpidem Zomepirac Zopiclone/Eszopiclone Hair ELISA Kits* Product No. Cocaine/BZE Methamphetamine Opiates Synthetic Opiates PCP THC *Not sold in the U.S. Oral Fluid ELISA Kits Product No. Cocaine/BZE Methamphetamine Opiates PCP THC Multi-Analyte Calibrator Set FOR FORENSIC USE ONLY jnew kits Ready-to-use (RTU) Assay Matrix specific calibrators included Other kits include positive and negative controls Neogen s ready-to-use or RTU test kits require no pre-dilution of conjugate prior to assay set-up. Neogen offers forensic assays that are ready to use and that do not require pre-dilution of reagents prior to assay setup. Matrix specific quantitative calibrators or qualitative positive or negative controls are assay-specific. See the product list or contact a forensic representative for details regarding a specific kit. 4 To order, call: (USA/Canada) or Fax: inform@neogen.com Web:
5 NeoSal Oral Fluid Collection System Neogen is proud to announce the release of our NeoSal Oral Fluid Collection System. Our oral fluid collection system is designed for maximum ease-of-use at the collection site and testing laboratory. Intended Use The NeoSal Oral Fluid Collection System is intended for the collection and transportation of oral fluid samples. Oral fluid samples collected with the NeoSal can be screened for drugs of abuse using Neogen s ELISA test kits, or other drug monitoring kits validated by the testing laboratory. NeoSal Key Features: Sample Volume Adequacy Indicator (SVAI) Stimulant-free collection pad Quick collection, typically 1-2 minutes Unique collector handle fits directly into buffer tube No additional sample processing required Automation friendly, buffer tube fits onto 12 x 75 sample racks Consistent drug recovery Oral Fluid ELISA Kits Neogen has expanded its forensic drug detection product line by adding panel of oral fluid ELISA kits for Cocaine/BZE, Methamphetamine, Opiates, PCP and THC. Features of Neogen s Oral Fluid ELISA Kits: Optimized with the NeoSal Oral Fluid Collection System High sensitivity Excellent reproducibility Compatible with automated and semi-automated equipment Available in 96 and 480 well assay NeoSal 25 Pack Product # Neogen s Oral Fluid ELISA Kits Kit Name Product No. Cocaine/BZE Methamphetamine Opiates PCP THC Multi-Analyte Calibrator Set FOR FORENSIC USE ONLY To order, call: (USA/Canada) or Fax: inform@neogen.com Web: 5
6 Hair ELISA Drug Kits Neogen has expanded its forensic drug detection product line by adding hair testing ELISA kits for the most commonly abused drugs on the illicit market Cocaine, Methamphetamine, Opiates, Synthetic Opiates, PCP, and THC. Using hair samples to detect drugs of abuse has become common practice because of its advantages over traditional sample types. Advantages include: Hair is easy to handle and store Collection is far less invasive than urine and blood Brief periods of non-use do not affect test results A larger window of drug detection Simple retesting Difficult to adulterate test results These advantages have led hair samples to be extensively used in workplace testing, as well as in other areas, such as legal cases, occupational and traffic medicine, and for historical research. Intended Use For the determination of trace quantities of drug analytes in human hair samples. Features of Neogen s Hair ELISA Kits: High sensitivity Excellent reproducibility Compatible with automated and semi-automated equipment Available in 96 and 480 well assay Neogen's Hair ELISA Kits Kit Name Product No. Cocaine Methamphetamine Opiates Synthetic Opiates PCP THC Not sold in the U.S. FOR FORENSIC USE ONLY Assay Principle Neogen s competitive direct ELISAs operate on the basis of competition between the drug or its metabolite in the sample and the drug-enzyme conjugate for a limited number of specific binding sites on the precoated microplate. The simple assay procedure includes the addition of samples, calibrators or controls to the antibody-coated microplate followed by the addition of the enzyme conjugate (HRP). During incubation, the drug in the sample and the drug enzyme conjugate compete for binding sites on the antibody- coated microplate. After the incubation, the microplate is washed and K-Blue Substrate (TMB) is added. After the substrate incubation, the test can be read visually or with a microplate reader. The extent of color development is inversely proportional to the amount of drug in the sample or control/calibrator. The absence of the drug in the sample will result in a blue color (yellow if acid stop is used), whereas the presence of the drug will result in decreased or no color development. 6 To order, call: (USA/Canada) or Fax: inform@neogen.com Web:
7 ELISA Reagents Neogen recognizes that additional substrates and reagents may be required for your application. Therefore, Neogen makes available the following products that are optimized with our test kits. Reagents for Immunoassays Product Shelf Life Product Features Available Sizes Product Number Enhanced K-Blue 48 months when stored at 4 C Develops blue color in the presence of HRP RTU (ready-to-use) Low background High consistency between lots 200 ml 500 ml 1 L Red Stop Solution 24 months when stored at 4 C Produces a dark purple-pink color for a minimum of 2 hours when added to the wells Non-acidic solution RTU (ready-to-use) 200 ml 500 ml 1 L EIA Buffer 12 months when stored at 4 C Dilutes enzyme conjugates, standards and samples RTU (ready-to-use) 500 ml 1 L Wash Buffer 12 months when stored at 4 C Washes all unbound enzyme conjugate, samples and standards from microplates Concentrated (10x) buffer 500 ml 1 L ß-Glucuronidase Reagent Pack 6 months when Reagent A is stored at 4 C and Reagent B is stored at -20 C Allows for better detection of total Lorazepam with Neogen s Benzodiazepine Group (Oxazepam/Clonazepam) Kit Single Reagent Pack Assay Procedure Well Antibodies Conjugate Sample drug Wash Unbound drug and drug conjugate Bound drug and drug conjugate Substrate More Analyte Less Analyte Plates are precoated with the antibody. The plate is ready for use. DO NOT WASH. A sample or control is added to each well. Next, the drug-enzyme conjugate is added and the mixture is incubated at room temperature. Wash the plate to remove all unbound materials. The bound materials now remain in the microplate. Add TMB substrate to each well and allow the color to develop. Qualitative results are obtained by measuring the absorbance reading at 450 nm or 650 nm if no acid stop is used. To order, call: (USA/Canada) or Fax: inform@neogen.com Web: 7
8 Automated Equipment Dynex Equipment The DSX Automated Four-Plate ELISA System and the DS2 Automated Two-Plate ELISA System were developed for small to medium-throughput processing of ELISAs with advanced automation and precise liquid-handling capabilities. Neogen s specially-designed Single Line Reporting Software enhances the DSX Revelation and DS2 Matrix software s reporting capabilities. The DSX and DS2 automated processing systems feature: Modular Design Flexible configuration, simple upgrades, and quick module replacement Process Security Instrument self-diagnostics supports unmanned operation Cover lock protects reagents and prevents interference Ease of Use Quick and easy installation, user-friendly control system, and minimal maintenance required Excellent Performance Precise liquid handling capability eliminates variations Hamilton Equipment The Microlab STAR line of workstations was developed based on 50 years of expertise in liquid handling for fast and efficient assay automation and sample preparation. These innovative instruments were specifically designed for regulated laboratories to ensure full GMP-compliant operation. The Microlab STAR automated liquid handling platform features: High throughput processing Advanced pipetting technology Easy-to-use VENUS software Flexible design to allow easy instrument configuration Broad range of testing applications In addition to the Microlab STAR workstation Neogen offers the STARlet (medium - large volume sample lab) and the NIMBUS (small - medium volume sample lab) automated work stations. Direct Purchase and Reagent Rental Program Neogen offers a full line of Dynex and Hamilton automated equipment for use with our ELISA test kits. The equipment is available for direct purchase or through our Reagent Rental Program, which provides laboratories access to automation with a lower initial investment. To take advantage of the Reagent Rental Program, customers agree to purchase a predetermined volume of test kits per year from Neogen, based on number of samples and required drug panel. Once an agreement is reached, Neogen will provide the automated equipment to the customer as a rental. The agreement is structured so the cost of the equipment rental, service contracts and test kits are merged, and spread over the duration of the agreement. The Reagent Rental Program has proven to be a successful way for many of our customers to meet their automation requirements, while at the same time satisfying their ELISA test kit needs. For assistance in determining your exact needs and selecting the best automated solution for your unique situation, or more details regarding the Reagent Rental Program, contact a Neogen sales representative. 8 To order, call: (USA/Canada) or Fax: inform@neogen.com Web:
9 Neogen ELISA Drug Detection Cross-reactivity Data Acetaminophen # Acetaminophen 100% Procainamide 2% Ethyl p-aminobenzoate 1% Oxyphenbutazone 0.7% Penicillin G-Procaine 0.3% Procaine 0.1% Methylene Blue 0.09% Amphetamine # d-amphetamine 100% d-methamphetamine 1000% N-desmethylselegiline 203% Fenfluramine 87% Ephedrine 56% Nylidrin 21% l-amphetamine 17% Mephentermine 14% Phentermine 11% Phenethylamine 10% Benzphetamine 8% Phenylpropanolamine 6% Isoxsuprine 6% MDMA (3,4-methylenedioxymeth-amphetamine) 2% Pseudoephedrine 2% Clenbuterol 2% Fencamfamine 2% α-ethyltryptamine 1% 4-Hydroxyamphetamine 0.6% p-hydroxymethamphetamine 0.5% Tuaminoheptane 0.5% MDA (3,4-methylenedioxy-amphetamine) 0.4% Phenylephrine 0.4% DOB 0.12% DOM 0.11% Amitriptyline 0.06% Quinidine 0.06% Tyramine 0.03% Imipramine 0.02% Glutethimide 0.01% Nortriptyline 0.01% (+/-) Octopamine 0.01% Diethylpropion <0.5% Hordenine <0.1% Pyrantel <0.1% Amphetamine Specific # d-amphetamine 100% p-hydroxyamphetamine 162% p-methoxyamphetamine 160% (+/-)-MDA 117% 4-Fluoroamphetamine 42% α-ethyltryptamine 5% Phentermine 1% Phenylpropanolamine 0.9% Metaraminol 0.4% S(-)-Cathinone 0.4% DOB 0.4% DOM 0.3% l-amphetamine 0.3% Phenethylamine 0.3% 2-Aminoheptane 0.2% Methylhexanamine 0.1% d-methamphetamine 0.1% N-Desmethylselegiline 0.1% Mephentermine 0.06% p-methoxymethamphetamine 0.03% (+/-)-MDMA 0.01% S(-)-Methcathinone 0.01% Amphetamine Ultra # d-amphetamine 100% N-desmethylselegiline 906% Fenproporex 731% d-methamphetamine 688% (-)-Ephedrine 49% Fenfluramine 33% Mephentermine 25% Nylidrin 14% 4-Fluoroamphetamine 13% l-amphetamine 7% Phenylpropanolamine 6% Phentermine 6% meta-chlorophenylpiperazine (mcpp) 6% Isoxsuprine 4% Benzphetamine 2% Methylhexanamine 1% R(+)-Methcathinone 1% (+/-)-MDMA 1% (+/-)-Pseudoephedrine 1% Fencamfamine 1% α-ethyltryptamine 0.8% S(-)-Methcathinone 0.7% S(-)-Cathinone 0.6% 2-Aminoheptane 0.6% Ampedramoff 0.6% Phenethylamine 0.5% p-hydroxyamphetamine 0.5% Selegiline 0.4% (+/-)MDA 0.4% Phendimetrazine 0.4% DOM 0.3% Clenbuterol 0.3% Metaraminol 0.2% p-methoxymethamphetamine 0.2% Salvinorin A 0.2% p-methyoxyamphetamine 0.1% DOB 0.1% Mephedrone 0.04% Hordenine 0.03% Pyrimethamine 0.03% Aminorex 0.03% Nicotine 0.02% Amitryptyline 0.02% Quinidine 0.02% Imipramine 0.01% Nortriptyline 0.01% (+/-) Synephrine 0.01% Barbiturate Group # Secobarbital 100% Amobarbital 757% Phenobarbital 469% p-oh Phenobarbital 316% Barbital 239% Butabarbital 131% Allobarbital 117% Butalbital 109% Aprobarbital 98% Pentobarbital 95% Mephobarbital 0.3% Methylene Blue 0.1% Glutethamide 0.1% Benzodiazepine Group (Oxazepam/Clonazepam) # Oxazepam 100% Diazepam 434% Estazolam 365% Nordiazepam 361% Alprazolam 346% Tetrazepam 264% Flurazepam 262% Lormetazepam 231% Prazepam 198% Temazepam 192% Phenazepam 190% Halazepam 173% Triazolam 171% 7-amino-Flunitrazepam 147% Diclazepam 142% Nitrazepam 141% Flubromazepam 120% N-desmethyl Flunitrazepam 119% Delorazepam 115% Flunitrazepam 110% Pyrazolam 106% Etizolam 87% Bromazepam 85% Clonazepam 79% Nimetazepam 74% Lorazepam 70% Midazolam 65% Clobazam 59% 7-amino-Clonazepam 39% alpha-hydroxy Alprazolam 25% Chlordiazepoxide 19% Benzylpiperazine # Benzylpiperazine 100% 1-(3-Trifluoromethyl)benzyl) piperazine 3% 1-(4-Trifluoromethyl)benzyl) piperazine 1% Trazodone 1% Nefazodone 0.20% Doxepin 0.13% Chlorpromazine 0.07% m-cpp 0.07% Isoxuprine 0.05% Pyrantel 0.03% N-[3-(Trifluoromethyl)phenyl] piperazine 0.03% Imipramine 0.02% Acepromazine 0.02% Orphenadrine 0.01% Amitriptyline 0.01% Nicotine 0.01% Buprenorphine # Buprenorphine 100% Diprenorphine 110% Norbuprenorphine 15% Etorphine 0.22% Butorphanol 0.16% Naltrexone 0.05% Thebaine 0.02% Nalorphine 0.02% Carisoprodol # Carisoprodol 100% Celecoxib # Celecoxib 100% Celecoxib Carboxylic Acid 143% Deracoxib 56% Citalopram # Citalopram 100% N-desmethylcitalopram 109% (S)-Citalopram 20% Acepromazine 2% Chlorpromazine 0.7% Fluoxetine 0.1% Imipramine 0.07% Amitriptyline 0.03% Paroxetine 0.03% Doxepin 0.03% Trimipramine 0.03% Nortriptyline 0.02% Fluvoxamine 0.02% Promazine 0.02% Orphenadrine 0.01% Sertraline 0.01% Duloxetine 0.01% Cocaine/Benzoylecgonine # Benzoylecgonine 100% Cocaine 133% Cocaethylene 124% m-hydroxycocaine 96% Ecgonine 0.4% Norcocaine 0.3% Ecgonine Methyl Ester 0.06% Cotinine CT-96-U (-) Cotinine 100% (+/-) Trans-3 -hydroxycotinine 3% (R/S) Norcotinine 0.89% Cotinine CT-96-S (-) Cotinine 100% (+/-) Trans-3 -hydroxycotinine 9% (R/S) Norcotinine 2% Detomidine # Detomidine 100% Carboxydetomidine 74% Medetomidine 26% Dextromethorphan # Dextromethorphan 100% 3-Methoxymorphinan 118% 3-Hydroxymorphinan 2% Dextrorphan 2% Promazine 0.05% Trimipramine 0.04% Orphenadrine 0.04% Imipramine 0.02% Nortriptyline 0.02% Amitriptyline 0.02% Acepromazine 0.01% Meperidine 0.01% Fentanil Group # Alfentanil 100% Sufentanil 270% Norsufentanil 119% Carfentanil 88% Remifentanil 76% Fentanyl 2% Thienylfentanyl 1% α-methylfentanyl 0.77% p-fluorofentanyl 0.19% 3-Methylfentanyl 0.08% Norfentanyl 0.04% Fentanyl # Fentanyl 100% p-fluorofentanyl 136% Thienylfentanyl 67% 3-Methylfentanyl 50% Acetyl Fentanyl 42% α-methylfentanyl 11% Carfentanil 6% Despropionylfentanyl 0.61% Lofenanil 0.20% Sufentanil 0.15% Norfentanyl 0.01% Fexofenadine/Terfenadine # Fexofenadine 100% Terfenadine 23% Hydroxyzine 0.97% Orphenadrine 0.20% Cetirizine 0.18% Hydroxyebastine 0.13% Carebastine 0.07% Doxepin 0.04% Amitryptyline 0.03% Imipramine 0.03% Nortriptyline 0.03% Promazine 0.03% Chlorpromazine 0.02% Ebastine 0.02% Trimipramine 0.01% Flunitrazepam # Flunitrazepam 100% 7-Aminoflunitrazepam 212% Diazepam 69% Clobazam 3% Clonazepam 2% Midazolam Maleate 1% 7-Aminoclonazepam 0.6% N-Desmethylflunitrazepam 0.5% Halazepam 0.4% Triazolam 0.3% Nitrazepam 0.3% Nordiazepam 0.2% Methylene Blue 0.02% Trimipramine 0.01% Fluoxetine # Fluoxetine 100% Norfluoxetine 67% Haloperidol # Haloperidol 100% Bromperidol 83% Trifluperidol 0.39% Risperidone 0.12% Spiperone 0.04% Fluanisone 0.03% Benperidol 0.01% Haloperidol Metabolites # Haloperidol Metabolite (II) ((+(-)-4- (4-chlorophenyl)-α-(4-fluorophenyl)-4- hydroxy-1-piperidinebutanol) 100% Bromperidol 157% Haloperidol 84% Haloperidol Metabolite (I) (4-(4-chlorophenyl) -4-hydroxypiperidine) 48% Azaperone 0.002% Hydrochlorothiazide # Hydrochlorothiazide 100% Trichlormethiazide 206% Chlorothiazide 8% Furosemide 0.24% Methylene Blue 0.01% Hydromorphone # Hydromorphone 100% 6-Acetylcodeine 143% Morphine 85% Hydrocodone 72% Dihydromorphine 56% Codeine 55% Ethylmorphine 54% Levorphanol 49% 6-Acetylmorphine 44% Morphine-6-glucuronide 39% Dihydrocodeine 38% Heroin 29% Thebaine 2% Levallorphan 0.5% Morphine-3-glucuronide 0.4% Oxymorphone 0.3% To order, call: (USA/Canada) or Fax: inform@neogen.com Web: 9
10 Neogen ELISA Drug Detection Cross-reactivity Data Oxycodone 0.2% Nalorphine 0.1% Normorphine 0.1% Norcodeine 0.04% Meperidine 0.02% Dextromethorphan 0.02% Amitriptylline 0.01% Doxepin 0.01% Ibuprofen # Ibuprofen 100% Flurbiprofen 0.3% Fenoprofen 0.06% Indoprofen 0.02% Suprofen 0.01% Ketamine # Ketamine 100% Norketamine 5% Methoxetamine 0.08% LSD # LSD 100% LAMPA 153% Fentanyl 56% Nor-/Nor-iso-LSD 36% Acetyl Fentanyl 13% Iso-LSD 3% 2-oxo-3-OH LSD 2% 2-oxo-3-OH LAMPA 1% Lobeline 0.08% Clenbuterol 0.07% Methylene Blue 0.06% Procaineamide 0.02% Meperidine Enhanced # Meperidine 100% Normeperidine 2% Anileridine 0.12% Diphenoxylate 0.06% Chlorpromazine 0.01% Methadone # Methadone 100% LAAM 113% Propiomazine 0.06% Trimeprazine 0.04% Promethazine 0.04% EDDP 0.03% Trimipramine 0.02% Imipramine 0.01% Orphenadrine 0.01% Acepromazine 0.01% Amitriptyline 0.01% Prochlorperazine 0.01% Promazine 0.01% Chlorpromazine 0.01% Methotrimeprazine 0.01% Methadone/LAAM # Methadone 100% LAAM 107% Dextromethorphan 0.18% Promethazine 0.15% Trimeprazine 0.11% Trimipramine 0.09% EDDP 0.04% Promazine 0.04% Propiomazine 0.03% Acepromazine 0.03% Chlorpromazine 0.03% Imipramine 0.02% Doxepin 0.02% Orphenadrine 0.02% Amitriptyline 0.02% Prochlorperazine 0.01% Methotrimeprazine 0.01% Meperidine 0.01% Methamphetamine/MDMA # d-methamphetamine 100% PMMA (p-methoxymethamphetamine) 1400% MDMA (3,4-Methylenedioxymethamphetamine) 212% p-hydroxymethamphetamine 77% l-methamphetamine 69% MDEA (Methylenedioxyethylamphetamine) 54% Mephentermine 13% Methoxyphenamine 8% Fenfluramine 7% N-desmethyselegiline 7% PMA (p-methoxyamphetamine) 4% Ephedrine 3% 4-Fluoroamphetamine 2% Mephedrone 2% MDA (3,4-Methylenedioxyamphetamine) 2% Pseudoephendrine 1% 4-Hydroxyamphetamine 0.6% d-amphetamine 0.6% Phenethylamine 0.5% α Ethyltryptamine 0.2% (+/-) Synephrine 0.2% Phentermine 0.2% Hordenine 0.2% Methylhexanamine 0.1% Nortriptyline 0.1% PPA (Phenylpropanolamine) 0.1% Penicillin G-Procaine 0.04% Fenproporex 0.04% Procaine 0.02% Doxepin 0.01% Methylphenidate # Methylphenidate 100% Ritalinc Acid 0.53% Naltrexone/Nalbuphine # Naltrexone 100% Nalbuphine 202% Naltriben 41% Butorphanol 28% Nalmefene 26% 6ß-Naltrexol 7% 3-Methoxynaltrexone 6% Naloxone 4% Nalorphine 1% Pentazocine 0.3% Codeine 0.02% Opiate Group # Morphine 100% 6-Acetylcodeine 195% Codeine 190% Morphine-3-Glucuronide 154% Heroin/Diacetylmorphine 154% 6-Acetylmorphine 146% Hydrocodone 122% Ethylmorphine 110% Nalorphine 76% Hydromorphone 66% Morphine-6-Glucuronide 37% Desomorphine 22% Thebaine 11% Levallorphan 7% Naloxone 2% Oxycodone 2% Levorphanol 2% Oxymorphone 1% Imipramine 0.4% Norcodeine 0.3% Trimipramine 0.3% Normorphine 0.2% Naltrexone 0.2% Acepromazine 0.05% Meperidine 0.02% Chlorpromazine 0.02% Promazine 0.01% Oxycodone/Oxymorphone # Oxycodone 100% Oxymorphone 88% Hydrocodone 27% Hydromorphone 21% Ethylmorphine 7% Codeine 5% Thebaine 3% 6-Acetylcodeine 3% Noroxycodone 3% 6-Acetylmorphine 2% Levorphanol 2% Heroin 2% Morphine 1% Noroxymorphone 0.2% Meperidine 0.03% Pentazocine # Pentazocine 100% Carfentanil 0.01% Phencyclidine (PCP) # Phencyclidine 100% 4-Hydroxyphencyclidine 2% Venlafaxine 0.18% Cyclobenzaprine 0.12% Orphenadrine 0.04% Amitriptyline 0.03% Promazine 0.03% Nortriptyline 0.02% Chlorpromazine 0.02% Imipramine 0.02% Doxepin 0.01% Methoxetamine 0.01% Methylene Blue 0.01% Trimipramine 0.01% Acepromazine 0.01% Phenytoin # Phenytoin 100% Hydroxyphenytoin 61% Propoxyphene # Propoxyphene 100% Norpropoxyphene 30% Salicylates # Salicylic Acid 100% 4-aminosalicylic Acid 121% Diflunisal 52% Gentisic Acid 37% Salicyluric Acid (2-Hydroxyhippuric Acid) 2% Sertraline # Sertraline 100% N-Desmethylsertraline 50% Methylene Blue 4% Fluoxetine 0.7% Pyrilamine 0.4% Paroxetine 0.3% Promazine 0.2% Chlorpromazine 0.2% Amitriptyline 0.2% Acepromazine 0.2% Nortriptyline 0.2% Doxepin 0.1% Pyrethamine 0.1% Trimipramine 0.1% Imipramine 0.1% Phenothiazine 0.02% Synthetic Cannabinoids (JWH-018) # JWH % JWH-073-N-(4-hydroxybutyl) metabolite 980% JWH-018 N-5-hydroxypentyl 754% JWH % JWH-018- N-pentanoic acid 613% JWH % AM % JWH-073 N-butanoic acid 426% (±) JWH-018-N-(4-hydroxypentyl) metabolite 392% AM % AM % JWH % AM-2201 N-(4-hydroxypentyl) metabolite 166% 1 -Naphthoyl Indole 153% JWH-018 N-(5-hydroxypentyl) ß-D-glucuronide 136% MAM % JWH hydroxyindole 126% AM % JWH % JWH % JWH hydroxyindole metabolite 60% JWH % JWH hydroxyindole metabolite 50% AM hydroxyindole metabolite 50% JWH % JWH % WIN 55,212-3 mesylate 11% JWH-081 6% JWH-210 5% JWH-250 N-(5-carboxypentyl) metabolite 2% JWH-250 N-(5-hydroxypentyl) metabolite 1% JWH-250 N-(4-hydroxypentyl) metabolite 1% RCS-4 N-(5-hydroxypentyl) metabolite 0.7% JWH % JWH % RCS-4 0.4% RCS-8 0.3% PB-22 N-(5-hydroxypentyl) metabolite 0.2% PB-22 N-pentanoic acid 0.12% 5-fluoro PB % PB-22 N-(4-hydroxypentyl) metabolite 0.11% PB % BB % Synthetic Cannabinoids (JWH-250) # JWH % JWH-250 N-(5-carboxypentyl) metabolite 160% JWH-250 N-(5-hydroxypentyl) metabolite 158% JWH-250 N-(4-hydroxypentyl) metabolite 123% JWH-073 N-(4-hydroxybutyl) metabolite 107% JWH-018 N-(5-hydroxypentyl) metabolite 104% JWH % (+)-JWH-018 N-(4-hydroxypentyl) metabolite 90% AM % AM % JWH % JWH % JWH-018 N-pentanoic acid 64% JWH-018 N-(5-hydroxypentyl) ß-D-glucuronide 59% JWH % JWH hydroxyindole metabolite 55% AM % JWH-073 N-butanoic acid metabolite 53% JWH % JWH % AM hydroxyindole metabolite 33% RCS-8 33% MAM % PB-22 N-(5-hydroxypentyl) metabolite 23% JWH hydroxyindole metabolite 21% 5-fluoro PB-22 19% PB-22 N-(4-hydroxypentyl) metabolite 19% 1 Napthoyl Indole 18% JWH hydroxyindole metabolite 16% PB-22 N-pentanoic acid 14% JWH % JWH % PB-22 11% JWH hydroxyindole metabolite 10% JWH % (+) WIN (mesylate) 8% JWH-081 7% BB-22 6% AM 2201 N-(4-hydroxypentyl) metabolite 6% JWH-398 4% JWH-210 1% AM 694 1% RCS-4 N-(5-hydroxypentyl) metabolite 0.8% RCS-4 0.7% XLR11 N-(4-hydroxybutyl) metabolite 0.01% Synthetic Cannabinoids (UR-144/XLR-11) # UR-144 N-pentanoic acid 100% A % UR-144 N-(5-hydroxypentyl) metabolite 89% (±)-UR-144 N-(4-hydroxypentyl) metabolite 62% XLR-11 45% A % XLR11 N-(4-pentenyl) analog 20% UR % UR-144 N-(2-hydroxypentyl) metabolite 11% XLR-11 N-(4-hydroxypentyl) metabolite 7% XLR11 Degradant 6% UR-144 Degradant 2% STS-135 (RM) 0.44% AKB48 N-(5-fluoropentyl) metabolite 0.33% AKB % JWH-250 N-(5-hydroxypentyl) metabolite 0.1% JWH % JWH-250 N-(5-carboxypentyl) metabolite 0.07% JWH-250 N-(4-hydroxypentyl) metabolite 0.07% JWH % AM % RCS % AM % JWH % JWH % AM % JWH-073 N-(4-hydroxybutyl) metabolite 0.01% JWH-018 N-(5-hydroxypentyl) metabolite 0.01% 10 To order, call: (USA/Canada) or Fax: inform@neogen.com Web:
11 Neogen ELISA Drug Detection Cross-reactivity Data Synthetic Cathinones (Methcathinone) R(+) Methcathinone 100% Mephedrone 167% Methedrone 150% Methylone 120% Buphedrone 103% 4-Fluoromethcathinone (Flephedrone) 94% 3-Fluoromethcathinone 75% (±) Methcathinone 73% Ethylone (bk-mdea) 43% Ethcathinone 40% 4-Methylethcathinone 35% Pentylone 2% Pentedrone 1% R(+) Cathinone 0.43% S(+) Methamphetamine 0.088% (±) MBDB (Butylone) 0.055% (-)-(S)-Cathinone 0.050% 1R,2S(-) Ephedrine 0.043% 1S,2R(+) Ephedrine 0.012% Diethylpropion 0.006% (±) N-Ethylcathinone ephedrine 0.006% R,R(-) Pseudoephedrine 0.005% MDPV < 0.003% PVP < 0.003% (±) MDA < 0.003% R(-) Amphetamine < 0.003% (+) Norpseudoephedrine < 0.003% (+)-N,N-Diethylnorephedrine < 0.003% (±) Phenylpropanolamine < 0.003% Naphyrone < 0.003% S,S(+)-Pseudoephedrine < 0.003% THC # THC-COOH 100% 8 -THC-COOH 88% 11-OH- 9 -THC 13% 8 -THC 5% 9 -THC 4% Cannabinol 2% Cannabidiol 0.01% Tramadol # Tramadol 100% O-Desmethyl Tramadol 22% N-Desmethyl Tramadol 7% N,N-Bisdesmethyl Tramadol 5% Trazodone # Trazodone 100% Nefazodone 20% m-cpp 0.7% N-(3-(Trifluoromethyl)phenyl)piperazine 0.3% 1-(3-(Trifluoromethyl)benzyl)piperazine 0.2% Doxepin 0.1% 1-(4-(Trifluoromethyl)benzyl)piperazine 0.07% Acepromazine 0.01% Imipramine 0.01% Tricyclics Group # Imipramine 100% Amitriptyline 464% Doxepin 364% Cyclobenzaprine 315% Dothiepin 232% Nortriptyline 155% Clomipramine 143% Protriptyline 44% Trimipramine 44% Promazine 21% Chlorpromazine 20% Desipramine 12% Perphenazine 9% Prochlorperazine 8% Cyproheptadine 4% Maprotiline 4% Triflupromazine 3% Fluphenazine 3% Trifluoperazine 3% Thioridazine 2% Orphenadrine 2% Acetophenazine 0.7% Acepromazine 0.6% Amoxapine 0.5% Clozapine 0.4% Thiethylperazine 0.2% Propionylpromazine 0.1% Olanzapine 0.05% Mesoridazine 0.04% Thiothixene 0.04% Glycopyrrolate 0.02% Nefopam 0.02% Hydroxyzine 0.02% Trazodone 0.01% Pyrilamine 0.01% Pyrimethamine 0.01% Zolpidem # Zolpidem 100% Carboxy-Zolpidem 0.02% Zopiclone/Eszopiclone # Zopiclone 100% Zopiclone-N-Oxide 100% N-Desmethyl-Eszopiclone 350% Eszopiclone 194% 6-(5-Chloro-2-pyridyl)-6,7-dihydro-7-hydroxy -5H-pyrrolo[3,4b]pyrazin-5-one 0.4% Zaleplon <0.04% Zolpidem <0.04% Oral Fluid Kits Cocaine Oral Fluid # Benzoylecgonine 100% m-hydroxybenzoylecgonine 140% Cocaethylene 59% Cocaine 58% Tropacocaine 4% Ecgonine 1% Norbenzoylecgonine 0.5% Ecgonine methyl ester 0.5% Methylene Blue 0.3% Anhydroecgonine methyl ester 0.2% Norcocaine 0.2% Methamphetamine Oral Fluid # S (+) Methamphetamine 100% PMMA (p-methoxymethamphetamine) 367% (+/-) MDMA (3,4,-Methylene-dioxymethamphetamine) 97% p-hydroxymethamphetamine 52% (+/-) MBDB (1,3-Benzodioxolyl-N-methylbutanamine) 43% (+/-) MDEA (Methylenedioxyethylamphetamine) 9% Mephentermine 5% (+/-) HMMA (4-hydroxy-3-methoxymandelic acid) 4% PMA (p-methoxyamphetamine) 3% Hydroxyephedrine 2% 1R, 2S (-)Ephedrine 2% R (-) Methamphetamine 2% (+/-) MDA (3,4-Methylenedioxyamphetamine) 0.9% (+) Amphetamine 0.6% Fenfluramine 0.5% Hordenine 0.5% Methoxyphenamine 0.5% (+) Pseudoephedrine 0.5% Phentermine 0.1% Nicotine 0.1% R (+) Methcathinone 0.1% Opiates Oral Fluid # Morphine 100% Ethylmorphine 162% Codeine 136% 6-Acetylcodeine 103% 6-Acetylmorphine 86% Heroin/Diacetylmorphine 86% Dihydrocodeine 39% Morphine-6-β-D-Glucuronide 35% Dihydromorphine 28% Morphine-3-β-D-Glucuronide 25% Thebaine 23% Hydrocodone 17% Hydromorphone 12% Levorphanol 5% Nalorphine 4% Oxymorphone 0.2% Oxycodone 0.2% Norcodeine 0.2% Meperidine 0.1% Diprenorphine 0.1% Normorphine 0.1% Norbuprenorphine <0.4% Buprenorphine <0.4% Noroxymorphone <0.4% PCP Oral Fluid # PCP 100% Metaphit 200% 4-Methoxy PCP (hydrochloride) 100% PCPr (hydrochloride) 4% PCP morpholine 3% 4-Hydroxy PCP 0.2% PCMPA (hydrochloride) 0.2% Furosemide 0.03% Promazine 0.02% Acepromazine 0.02% THC Oral Fluid # (-) 9 -THC 100% 11-nor-9-carboxy- 8 -THC 252% (+/-) 11-hydroxy- 9 -THC 146% (-) 11-nor-9-carboxy- 9 -THC 138% (+/-) 11-nor-9-carboxy- 9 -THC 87% (-) 8 -THC 62% Cannabinol 22% Hair Kits Cocaine Hair # Cocaine 100% Benzoylecgonine isopropyl ester 167% Cocaethylene 133% Benzoylecgonine 83% Meta-Hydroxybenzoylecgonine 71% Ecgonine 0.6% Norbenzoylecgonine 0.3% Norcocaine 0.2% Norcocaethylene 0.2% Ecgonine methyl ester <0.1% Anhydroecgonine methyl ester <0.1% Anhydroecgonine <0.1% Atropine <0.1% Methamphetamine Hair # (+/-) MDMA 100% PMMA 250% S (+) Methamphetamine 53% (+/-) MBDB 33% p-hydroxymethamphetamine 33% (+/-) MDEA 17% Mephentermine 7% (+/-) 4-Hydroxyephedrine 5% 1R,2S (-) Ephedrine 3% (-) Methamphetamine 3% (+/-) HMMA 2% PMA 2% (+/-) MDA 0.8% Fenfluramine 0.6% (+) Pseudoephedrine 0.6% Methoxyphenamine 0.3% (-) Amphetamine 0.2% 1S,2R (+) Ephedrine <1.3% 4-Fluoroamphetamine HCl <1.01% (+) Amphetamine <0.1% Diphenhydramine <0.1% Phenylpropanolamine <0.1% R,R (-) Pseudoephedrine <0.1% Phendimetrazine <0.1% R (+) Methcathinone <0.1% Phentermine <0.1% R ( +) Cathinone <0.1% Labetalol <0.1% (-) Phenylephrine <0.1% (+/-) 2,5-Dimethoxy-4-bromoamphetamine <0.1% Opiates Hair # Morphine 100% Dihydrocodeine 243% Heroin 142% Codeine 121% 6-Monoacetylmorphine 85% Morphine-6-Glucuronide 8% Morphine-3-Glucuronide 8% Hydrocodone 5% Hydromorphone 3% Oxycodone 0.06% Oxymorphone 0.04% Synthetic Opiates Hair # Oxycodone 100% Hydrocodone 120% Oxymorphone 20% Dihydrocodeine 12% 6-Acetylcodeine 8% Codeine 7% Ethylmorphine 6% Hydromorphone 5% Heroin 2% Dihydromorphine 2% Levorphanol 2% 6-Monoacetylmorphine 2% Morphine 1% Noroxycodone 1% Thebaine 0.8% Morphine-3-glucuronide 0.2% Naloxone 0.1% Morphine-6-glucuronide <1.5% Norbuprenorphine <1.5% Buprenorphine <1.5% Noroxymorphone <1.5% Nalorphine <0.2% Normorphine <0.2% Diprenorphine <0.2% Dextromethorphan <0.2% Naltrexone <0.2% Naltriben <0.2% Nalmefene <0.2% Apomorphine <0.2% 3-Methoxy-naltrexone <0.2% PCP Hair # Phencyclidine 100% Metaphit 60% Phencyclidine Morpholine 20% 4-Hydroxy-Phencyclidine 5% THC Hair # THC 100% (-)-11-nor- 9 -THC-9-COOH 417% (-)-11-nor- 9 -THC-9-COOH-glucuronide 313% (-)-11-nor- 8 -THC-9-COOH 167% (+/-)-11-nor- 9 -THC-9-COOH 125% (+/-)-11-Hydroxy- 9 -THC 100% Cannabinol 71% 8 -THC 71% Cannabidiol 0.17% To order, call: (USA/Canada) or Fax: inform@neogen.com Web: 11
12 References V. Spiehler, D. Isenschmid, P. Mathews, P. Kemp, T. Kupiec. Performance of a Microtiter Plate ELISA for Screening of Postmortem Blood for Cocaine and Metabolites. J. Analytical Toxicol. 27: (2003). V. Spiehler, L. DeCicco, R. McCutcheon, T. Kupiec, P. Kemp. Screening Postmortem Whole Blood for Oxycodone by ELISA Response Ratios. J. Forensic Sci. 49, No. 3: (2004). T. Kupiec, L. DeCicco, V. Spiehler, G. Sneed, P. Kemp. Choice of an ELISA Assay for Screening Postmortem Blood for Amphetamine and/or Methamphetamine. J. Analytical Toxicol. 26: (2002). P. Kemp, G. Sneed, T. Kupiec, V. Spiehler. Validation of a Microtiter Plate ELISA for Screening of Postmortem Blood for Opiates and Benzodiazepines. J. Analytical Toxicol. 26: (2002). H.R. Wallage and J-P.F.P. Palmentier. Hydromorphone-Related Fatalities in Ontario. J. Analytical Toxicol. 30: (2006). N. Harun, R.A. Anderson, E.I. Miller. Validation of an Enzyme-Linked Immunosorbent Assay Screening Method and a Liquid Chromatography-Tandem Mass Spectrometry Confirmation Method for the Identification and Quantification of Ketamine and Norketamine in Urine Samples from Malaysia. J. Analytical Toxicol. 33: (2009). A. Negrusz, P. Adamowicz, B.K. Saini, D.E. Webster, M.P. Juhascik, C.M. Moore, R.F. Schlemmer. Detection of Ketamine and Norketamine in Urine of Nonhuman Primates after a Single Dose of Ketamine using Microplate Enzyme-Linked Immunosorbent Assay (ELISA) and NCI-GC-MS. J. Analytical Toxicol. 29: (2005). Brzenski AB, Phan J, Yaksh TL, Rossi SS, Benumof JL (2013) Intravenously Administered Fentanyl is Not Detectable in Exhaled Breath. Analg Resusc: Curr Res 2:1. doi: / x L. Reidy, B. Nolan, A.R. Ramos, H.C. Walls, B.W. Steele. Zolpidem Urine Excretion Profiles and Cross-Reactivity with ELISA Kits in Subjects Using Zolpidem or Ambien CR as a Prescription Sleep Aid. J. Analytical Toxicol. 35: (2011). P. Adamowicz, B. Tokarczyk, R. Stanaszek, M. Slopianka. Fatal Mephedrone Intoxication A Case Report. J. Analytical Toxicol. 37:37-42 (2013). doi: /jat/bks085 N.B. Tiscione, X. Shan, I. Alford, D.T. Yeatman. Quantitation of Opioids in Whole Blood by Electron Impact-Gas Chromatography Mass Spectrometry. J. Analytical Toxicol. 35: (2011). 944 Nandino Boulevard Lexington, KY (USA/Canada) inform@neogen.com Neogen Corporation, Neogen and K-Blue are registered trademarks of Neogen Corporation, Lansing, Michigan. All other trademarks are the property of their respective companies. LS
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