Safety Data Sheet Carbol Fuchsin

Transcription

1 Safety Data Sheet Carbol Fuchsin SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND THE COMPANY/ UNDERTAKING 1.1 Product Identifier Trade Name Product # SDS # Carbol Fuchsin 38016SS8A 38017SS6 186 SDS Date August 22, Relevant Identified Uses of the Substance or Mixture and Uses Advised Against Product Use: Histology Stain Uses Advised Against: All other uses. 1.3 Details of the Supplier of the Substance or Mixture Manufacturer/Preparer: Leica Biosystems Richmond, Inc Route 12 Richmond, IL Leica Biosystems Canada, Inc. 83 Terracon Place Winnipeg, Manitoba R2J 4B Emergency Telephone Number Emergency Spill Information (CHEMTREC) International calls (call collect) Other Product Information: SECTION 2: HAZARDS IDENTIFICATION 2.1 Classification of the Substance or Mixture CLP/GHS Classification (1272/2008): Physical: Health: Environmental Not Hazardous Skin Corrosion Category 1B Eye Damage Category 1 Germ Cell Mutagen Category 2 Carcinogen Category 1B Specific Target Organ Toxicity Repeat Exposure Category 2 Not Hazardous EU Classification (67/548/EEC): C, Xn, Carc. Cat 2, Muta. Cat.3, R20/21/22, R34, R45, R Label Elements DANGER! Contains phenol and basic fuchsin Page 1 of 9

2 Hazard Phrases H314 Causes sever skin burns and eye damage H341 Suspected of causing genetic defects. H350 May cause cancer H373 May cause damage to liver, kidneys, and eyes through prolonged or repeated exposure. Precautionary Phrases P201 Obtain special instructions before use. P202 Do not handle until all safety precautions have been read and understood. P260 Do not breathe mist/vapours/spray. P264 Wash exposed skin thoroughly after handling. P280 Wear protective gloves/protective clothing/eye protection/face protection. P301+P330+P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. P303+P361+P353 IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. P304 + P340 IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. P310 Immediately call a POISON CENTER or doctor/physician. P363 Wash contaminated clothing before reuse. P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P308 + P313 IF exposed or concerned: Get medical advice/ attention. P405 Store locked up. P501 Dispose of contents/container in accordance with local and national regulations. 2.3 Other Hazards: None SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substances Chemical Name CAS Number / EINECS Number / REACH Reg. Number Glycerol Dimethyl Sulfoxide Ethanol Phenol Basic Fuchsin % (w/w) See Section 16 for full text of GHS and EU Classifications. EU Classification (67/548/EEC) <10 Not classified as dangerous <10 Not classified as dangerous CLP/GHS Classification (1272/2008) Not classified as hazardous Not classified as hazardous <10 F R11 Flammable Liquid Category 2 (H225) <5 T, C, Muta. Cat.3, Germ Cell Mutagen Category 2 R23/24/25, R34, (H341) R68 Acute Toxicity Category 3 (H331, H311, H301) Specific Target Organ Toxicity Repeat Exposure Category 2 (H373) Skin Corrosive Category 1B (H314) Acute Aquatic Toxicity Category 3 (H412) <5 Carc. Cat 2; R45 Carcinogen Category 1B (H350) Page 2 of 9

3 SECTION 4: FIRST AID MEASURES 4.1 Description of First Aid Measures First Aid Eye contact: Immediately flush eye with water for at least 20 minutes while lifting the upper and lower lids. Get immediate medical attention. Skin contact: Immediately flush skin with water while removing contaminated cloths. Get immediate medical attention. Launder contaminated clothing before reuse. Inhalation: Ingestion: Remove victim to fresh air. Get medical attention if irritation persists. If breathing is difficult have qualified individual administer oxygen and get immediate medical attention. If breathing stops, give artificial respiration and get immediate medical attention. Do not induce vomiting unless directed to do so by medical personnel. If the victim is conscious and alert, have them rinse their mouth with water. Never give anything by mouth to an unconscious or drowsy person. Get immediate medical attention. See Section 11 for more detailed information on health effects. 4.2 Most Important symptoms and effects, both acute and delayed: Causes burns to eyes and skin. Inhalation of vapors may cause nervous system effects including dizziness, drowsiness, nausea, vomiting, visual disturbances and unconsciousness. Harmful if swallowed. Ingestion causes burns of the upper digestive and respiratory tract. 4.3 Indication of any immediate medical attention and special treatment needed: Immediate medical treatment is required for all significant exposures. SECTION 5: FIREFIGHTING MEASURES 5.1 Extinguishing Media: Use dry chemical, foam, carbon dioxide (CO2), or water spray. 5.2 Special Hazards Arising from the Substance or Mixture Unusual Fire and Explosion Hazards: This product contains a small amount of flammable liquid. Flammable vapors may collect in low, or confined areas. Combustion Products: Thermal decomposition can lead to release of carbon and sulfur oxides. 5.3 Advice for Fire-Fighters: Self-contained breathing apparatus and protective clothing should be worn in fighting large fires involving chemicals. SECTION 6: ACCIDENTAL RELEASE MEASURES 6.1 Personal Precautions, Protective Equipment and Emergency Procedures: Evacuate spill area. Ventilate the area. Wear appropriate protective clothing. Eliminate all ignition sources and ventilate the area. Prevent entry into basements or confined areas. 6.2 Environmental Precautions: Prevent entry in storm sewers and waterways. Report spill as required by local and federal regulations. Page 3 of 9

4 6.3 Methods and Material for Containment and Cleaning Up: Contain and collect with an inert absorbent and place into a suitable container for disposal. 6.4 Reference to Other Sections: Refer to Section 8 for personal protective equipment and Section 13 for disposal information. SECTION 7: HANDLING and STORAGE 7.1 Precautions for Safe Handling: Prevent contact with eyes, skin and clothing. Do not breathe vapors or mists. Use only with adequate ventilation. Wash thoroughly after handling. Keep containers closed when not in use. Keep product away from heat, sparks and all other sources of ignition. Keep containers closed when not in use. 7.2 Conditions for Safe Storage, Including any Incompatibilities: Store in a cool, dry well-ventilated area. Protect containers from physical damage. Keep containers closed when not in use. Store away from oxidizers and other incompatible materials. Empty containers retain product residues. Follow all SDS precautions in handling empty containers 7.3 Specific end use(s): Industrial uses: None identified Professional uses: Histology Stain SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1 Control Parameters: Chemical Name US OEL EU IOEL UK OEL Germany OEL Glycerol 10 mg/m3 TWA ACGIH TLV None Established 10 mg/m3 TWA 50 mg/m3 TWA (as mist) 100 mg/m3 STEL 15 mg/m3 TWA OSHA PEL (Total dust) 5 mg/m3 TWA OSHA PEL (Respirable Fraction) Dimethyl Sulfoxide 250 ppm AIHA WEEL None Established None Established 50 ppm TWA 100 ppm STEL Ethanol 1000 ppm TWA OSHA PEL 1000 ppm STEL ACGIH TLV None Established 1000 ppm TWA 500 ppm TWA 1000 ppm STEL Phenol 5 ppm TWA ACGIH TLV 2 ppm TWA 2 ppm TWA 2 ppm TWA (skin) 4 ppm STEL 4 ppm STEL 5 ppm TWA OSHA PEL (skin) Basic Fuchsin None Established None Established None Established None Established Refer to local or national authority for exposure limits not listed above. Chemical Name Glycerol Dimethyl Sulfoxide Ethanol Phenol Biological Limit Value None Established None Established None Established Phenol in urine 250 mg/g creatinine, end of shift (ACGIH) Page 4 of 9

5 Basic Fuchsin None Established 8.2 Exposure Controls: Recommended Monitoring Procedures: Ethanol: Collection on charcoal tubes with analysis by gas chromatography. Phenol: Collection on XAD-7 or ORBO 47 analysis by HPLC or gas chromatography. Appropriate Engineering Controls: Use with adequate local exhaust ventilation to maintain exposure levels below the occupational exposure limits. Personal Protective Measures Eye/face Protection: Wear safety glasses or chemical goggles. Face shield should be worn if splashing is possible. Skin Protection: Impervious clothing as needed to prevent skin contact. Hands: Impervious gloves recommended (Teflon), or multi-laminate. Respiratory Protection: None needed with adequate ventilation. If the occupational exposure limit is exceeded, an organic vapor respirator appropriate for the form and concentration of the contaminants should be used. Selection of respiratory protection depends on the contaminant type, form and concentration. Select in accordance with OSHA or other applicable regulations and good Industrial Hygiene practice. Other protection: Eye wash and suitable washing facilities should be available in the work area. SECTION 9: PHYSICAL and CHEMICAL PROPERTIES 9.1 Information on basic Physical and Chemical Properties Appearance: Red liquid. Odor: Phenolic odor Odor Threshold: Not established ph: Not applicable Melting/Freezing Point: Not available Boiling Point: 78.3ºC (173.0 F ) Flash Point: : >100 C (212 F ) Evaporation Rate: Not determined Lower Flammability Limit: 1.7% (Phenol) Vapor Pressure: Not available Upper Flammability Limit: 19% (Ethanol) Vapor Density(Air=1): Not available Relative Density: 0.95 Solubility: Soluble in water Octanol/Water Partition Coefficient: Not available Autoignition Temperature: Not available Decomposition Temperature: Not established Viscosity: Not established VOC Content (%): 42 Explosive Properties: Vapors may be explosive in confined areas. Oxidizing Properties: None Specific Gravity (H 2 O= 1): 0.95 Molecular Formula: Mixture Molecular Weight: Mixture 9.2 Other Information: None available SECTION 10: STABILITY and REACTIVITY 10.1 Reactivity: This material is not reactive under normal conditions Chemical Stability: Normally stable Possibility of Hazardous Reactions: Reaction with oxidizers may generate heat Conditions to Avoid: Avoid heat, sparks, flames, and all other sources of ignition. Page 5 of 9

6 10.5 Incompatible Materials: Avoid strong oxidizing agents, acids, bases, acid chlorides, phosphorus halides, reducing agents Hazardous Decomposition Products: Thermal decomposition can lead to release of carbon oxides and sulfur oxides. SECTION 11: TOXICOLOGICAL INFORMATION 11.1 Information on Toxicological Effects: Potential Health Effects: Eye Contact: May cause severe irritation or burns with redness, tearing and stinging. May cause corneal damage. Skin contact: May cause severe skin irritation and burns. Phenol may be absorbed through the skin in harmful amounts. Inhalation: Ingestion: Inhalation of mists may cause mucous membrane and upper respiratory tract irritation with coughing and sore throat. May cause central nervous system effects including headache, weakness, dizziness, confusion and unconsciousness. Ingestion may cause burns to the mouth, throat and stomach, abdominal pain, nausea, vomiting and diarrhea and central nervous system effects including dizziness, drowsiness, visual disturbances and unconsciousness. May cause damage to the kidney and liver. Acute toxicity: Glycerol: LD50 oral rat 12,600 mg/kg; LD50 skin rabbit >10,000 mg/kg; LC50 inhalation rat >570 mg/m3/ 1 hr Dimethyl Sulfoxide: LD50 oral rat 14,500 mg/kg; LD50 skin rat 40,000 mg/kg Ethanol: LD50 oral rat 7,060 mg/kg; LC50 inhalation rat 20,000 ppm/10 hr Phenol: LD50 oral rat 317 mg/kg; LD50 skin rabbit 630 mg/kg Basic Fuchsin: LD50 oral mouse 5,000 mg/kg; Skin corrosion/irritation: No data available for mixture. Phenol is corrosive to skin. Eye damage/ irritation: No data available for mixture. Phenol is expected to be corrosive to eyes and cause eye damage. Respiratory Irritation: No data available for mixture. High concentrations of vapors may be irritating to the respiratory system. Respiratory Sensitization: No data available for mixture. None of the components are respiratory sensitizers. Skin Sensitization: No data available for mixture. None of the components are skin sensitizers. Germ Cell Mutagenicity: No data available for mixture. Phenol was negative in the AMES test but positive at high doses in a CHO assay, a chromosome aberration assay and a mouse lymphoma cell assay. Carcinogenicity: Ingestion of alcoholic beverages is known to cause cancer in humans (IARC group 1). Basic Fuchsin is classified by IARC as Possibly Carcinogenic to Humans (Group 2B) and Reasonably Anticipated to be a Human Carcinogen by NTP. Reproductive Toxicity: No data available for mixture. Ethanol is known to cause developmental toxicity when intentionally ingested during pregnancy. Phenol was shown to cause reproductive effects only at doses that were toxic to the mother. Page 6 of 9

7 Specific Target Organ Toxicity: Single Exposure: Observed effects from acute exposure to phenol may include: shock, delirium, coma, pulmonary distress, phenolic breath, scanty/dark urine, and death. Repeat Exposure: Ethanol when consumed as a beverage has been found to cause damage to the liver, nervous system and reproductive system. Protracted or chronic exposure to phenol usually results in major damage to the liver, kidneys, and eyes. SECTION 12: ECOLOGICAL INFORMATION 12.1 Toxicity: Glycerol: LC50 Goldfish >5,000 mg/l/24 hr Dimethyl Sulfoxide: LC50 rainbow trout 53,000 mg/l/24 hr; LC50 Fathead minnow 36,000 mg/l/96 hr Ethanol: LC50 rainbow trout mg/l/96 hr; LC50 daphnia magna mg/l/48 hr; EC50 Chlorella pyrenoidosa (Green algae; growth inhibition) 9310 mg/l/48 hr Phenol: LC50 Fathead minnow mg/l/48-96 hr; LC50 Daphnia magna 17 mg/l/24-48 hr Basic Fuchsin: None established Persistence and degradability: Ethanol and phenol readily biodegradable in screening tests Bioaccumulative Potential: Ethanol and dimethyl sulfoxide are expected to have a low potential for bioaccumulation. The rapid elimination of phenol suggests that bioaccumulation of phenol is unlikely Mobility in Soil: Glycerin, dimethyl sulfoxide, ethanol and phenol are expected to have a high mobility in soil Results of PVT and vpvb assessment: Not required Other Adverse Effects: No data available. SECTION 13: DISPOSAL CONSIDERATIONS 13.1 Waste Treatment Methods: Dispose in accordance with local, state and national regulations. SECTION 14: TRANSPORTATION INFORMATION 14.1 UN Number 14.2 UN Proper Shipping Name 14.3 Hazard Class(s) 14.4 Packing Group US DOT Not regulated No Canadian Not regulated No TDG EU Not regulated No ADR/RID IMDG Not regulated No IATA/ICAO Not regulated No 14.6 Special Precautions for User: None 14.7 Transport in Bulk According to Annex III MARPOL 73/78 and the IBC Code: Not applicable SECTION 15: REGULATORY INFORMATION 14.5 Environmental Hazards 15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance or Mixture Page 7 of 9

8 INTERNATIONAL INVENTORIES EPA TSCA INVENTORY: All of the components are listed on the TSCA inventory. CANADIAN ENVIRONMENTAL PROTECTION ACT: All of the ingredients are listed on the Canadian Domestic Substances List. EUROPEAN UNION: All of the components of this product are listed on the European Inventory of New and Existing Chemical Substances (EINECS) inventory. CHINA: All of the ingredients are listed on the Chinese chemical inventory. NEW ZEALAND: All of the components of this product are listed on the New Zealand Inventory of Chemicals (NzloC). PHILIPPINES: All of the components of this product are listed on the Philippine Inventory of Chemicals and Chemical Substances (PICCS). U.S. REGULATIONS OSHA HAZARD CLASSIFICATION: Corrosive, Target Organ Effects, Carcinogen EPA SARA 302: This product contains Phenol which is regulated under SARA Section 302. CERCLA Section 103: The RQ for the product, based on the RQ for Phenol (5% maximum) of 1000 lbs, is 20,000 lbs. Many states have more stringent release reporting requirements. Report spills required under federal, state and local regulations. EPA SARA 311 HAZARD CLASSIFICATION: Acute Health, Chronic Health EPA SARA 313: This product contains the following chemicals that are regulated under SARA Title III, section 313: Phenol <5% CALIFORNIA PROPOSITION 65: This product contains the following chemicals which are known to the State of California to cause cancer or reproductive toxicity: Basic Fuchsin (cancer), Methanol <1% (Reproductive). INTERNATIONAL REGULATIONS WHMIS CLASSIFICATION: Class D-1-B, Class D-2-A, Class E SECTION 16: OTHER INFORMATION Revision History: Updated Logo and website. EU Classes and Risk Phrases for Reference (See Sections 2 and 3) Carc Cat 2 Carcinogen Category 2 Muta. Cat 3 Mutagen Category 3 F Highly Flammable T Toxic C Corrosive Xn Harmful R11 Highly Flammable R20/21/22 Harmful by inhalation, in contact with skin and if swallowed. R23/24/25 Toxic by inhalation, in contact with skin and if swallowed. R34 Causes burns R45 May cause cancer. Page 8 of 9

9 R68 Possible risk of irreversible effects CLP/GHS Classification and H Phrases for Reference (See Section 3) H225 Highly flammable liquid and vapour. H301 Toxic if swallowed. H311 Toxic in contact with skin H314 Causes severe skin burns and eye damage. H331 Toxic if inhaled. H341 Suspected of causing genetic defects. H350 May cause cancer. H373 May cause damage to liver, kidneys, and eyes through prolonged or repeated exposure. H412 Harmful to aquatic life with long lasting effects. NFPA Rating: Health: 2 Fire: 0 Instability: 0 HMIS Rating: Health: 2 Fire: 0 Physical Hazard: 0 This Safety Data Sheet has been prepared in accordance with the REACH regulation in the EU and the Globally Harmonized System for the Classification and Labeling of Chemicals (GHS). It complies with the requirements of the Canadian Controlled Products Regulations and US 29CFR To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries makes any warranty of merchantability or any other warranty, expressed or implied, which respect to such information, and we assume no liability resulting from its use. In no event shall Leica Biosystems be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages resulting from use of or reliance upon this information. Page 9 of 9