Structure and distribution of mucosal immunological tissue and cell in reproductive duct of female t wo2humped camel

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1 2001, 24 (4) : 7983 Journal of Nanjing Agricultural University (, ) : 12, ( IEL),, (LPL),,,, ( ),,, : ; ; ; ; ; ; ; ; : S : A : 1000Ο2030 (2001) 04Ο0079Ο05 Structure and distribution of mucosal immunological tissue and cell in reproductive duct of female t wo2humped camel Chen Qiusheng, Su Zehong and Chen Xiaowu (College of Veterinary Medicine, Nanjing Agric Univ, Nanjing , China) Abstract : Structure and distribution of the mucosal immunological tissue and cells in reproductive duct of female two2humped camels were investigated with microscope and electro2microscope. Intraepithelial lymphocytes ( IEL) were scattered extensively in the mucosal epitheli2 um and gland epithelium of the oviduct, uterus and vagina, and lymphocytes infiltration in the posterior segment of the cervix uteri and the anterior segment of the vagina was even occurred. There were some lamina propria lymphocytes (LPL), plasma cells, macrophages and meutraphilic granulocytes in lamina propria along the duct. The special eosinophil cells which were suggested to be dendritic cells (DC) were appeared in groups in the lymph nodule arising in lamina propria of the uterine. There were also some neutrophilic granulocytes among the epithelium of mucosal and gland and in the glandular cavity of cervix uteri. Mast cells in groups were observed in deeper lamina propria and upper musclar layer of the uterus but they were not showed in that of the pregnant camels. The results above suggested that the repro2 ductive duct of the female camel may possess an active mucosal immunization. Key words : two humped camel ; female ; reproductive duct ; mucosal ; immunology ; tissue ; cell ; structure ; distribution,,, 80 % [1 ],,, ( ) [2 ],,,,,, : 2001Ο06Ο17 : ( ) ' China Academic Journal Electronic Publishing House. All rights reserved.

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3 4 : Fig. 1 Structure of mucosal immunological tissue and cell in reproductive duct of female two2humped camel

4 : ( ) 100 ; 2. : ( ), ( ) 400 ; 3. : ( ), ( ), ( ), ( ) 400 ; 4. : ( ), ( ), ( ) 400 ; 5. : ( ), ( ) 400 ; 6. ( ) : ( ) 100 ; 7. : ( ), ( ), ( ), ( ) 100 ; 8. 2 : ( ) ( ), ( ) 400 ; 9. ( ) : ( ), ( ) 100 ; 10. : ( ), ( ) ; 11. : ( ), ( 19 H1E ) 1. The fimbriae tubae of the oviduct : Group of immunocytes ( ) 100 ; 2. The isthmus of the oviduct : IEL ( ), the lymphocyte in lamina propria ( ) 400 ; 3. Lamina propria of uterine horn : Lymphatic nodule ( ), uterine gland ( ), a group of special cells ( ), IEL in gland epithelium ( ) 400 ; 4. Mucous layer of uteria corpus : IEL ( ), uterine gland ( ), IEL in gland epithelium ( ) 400 ; 5. Uterocervical mucous : IEL ( ), neutrophil leucocytes ( ) 400 ; 6. Cervix (posterior segment) : Neutrophil leucocytes ( ) in the gland cavity 100 ; 7. Cervix : Mucous epithelium ( ), immunocytes in upper lamina propria ( ), uterocervical gland ( ), lymphatic nodule ( ) 100 ; 8. Junctional part of cervix and vagina : The immunocytes ( ) are densily distributed beneath the lining epithelium, lymphocytes infiltrated ( ) in some segments of the epithelium ( ) 400 ; 9. Vagina (anterior segment) : Accumulated immunocytes ( ) in upper layer of lamina propria, lymphocytes infiltration ( ) in the epithelium 100 ; 10. Electronograph of mast cell ( ) and plasma cell ( ) in lamina propria of uterine horn ; 11. Electronograph of the gland epithelium ( ) in uterine horn, the basement membrane of the epithelium is not clear (129 H E staining) 214,,,,, (1Ο8), (1Ο9),, (1Ο11), 3 Veazey, 80 % IEL CD3 + CD103 + T NK, 6 %CD20 + B, IEL, [3, ] LPL, TB, B IgA, IgGIgM, [4 ],,, ( IEL),,, (LPL), IEL LPL, IEL,, IEL,, (APC), (DC), B, B,,,, IgAIgA [5 IgA, ],,,,

5 4 : 83,,, B IgA,, IEL,, :,, ; ; [6, ],,,,, [7, ],,,, : [1 ] 1 [J ]., 2000, 23 (3) : 143Ο145. [2 ] Camerini V, Sydora B C, Arand R, et al. Generation of intestinal mucosal lymphocyte in SCID mice reconstituted with mature thymusοderived T cell [J ]. J Immunol, 1998, 160 : 2608Ο2618. [3 ] 1 [J ]., 1999, 22 (5) : 255Ο259. [4 ] Husband A J, Mesteky J, Moldoveanu Z, et al. Regulation of mucosal IgA respones in vivo : cytokines and aduvants [J ]. Vet Immunol Immunopathol, 1996, 54 : 179Ο186. [5 ] Kagnoff M E. Mucosal immunology : new frontiers [J ]. Immunology Today, 1996, 17 : 57Ο59. [6 ],,, 1 [J ]., 2000, 23 (3) : 225Ο227. [7 ],,, 1 [J ]., 1998, 29 (2) : 207Ο210. :