Clinical Chemistry
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1 Western Technical College Clinical Chemistry Course Outcome Summary Course Information Description Career Cluster Instructional Level Total Credits 5.00 Introduces Clinical Chemistry techniques and procedures for routine analysis using photometric, potentiometric, and separation techniques. Topics in this course include pathophysiology and methodologies for carbohydrate, lipids, proteins, renal function and blood gas analysis. Additional topics include hepatic, bone, cardiac markers, tumor markers, endocrine function, fetal function, miscellaneous body fluids, and toxicology. Health Science Associate Degree Courses Textbooks Clinical Chemistry - with Access. 8th Edition. Copyright Bishop, Michael L. Publisher: Lippincott Williams & Wilkins. ISBN-13: Required. Core Abilities 1. Apply mathematical concepts. 2. Demonstrate ability to think critically. 3. Transfer social and natural science theories into practical applications. 4. Use technology effectively. Course Competencies 1. Perform lipid tests 1.1. by completing a lipid report to document a lipid panel analysis Course Outcome Summary - Page 1 of 6
2 1.a. Identify the lab tests used to evaluate lipid levels 1.b. Explain methods of analysis used to evaluate lipid levels<br /> <br /> 1.c. Identify the reference ranges of the major lipids discussed 1.d. Explain factors associated with diet to lipoprotein analysis 1.e. Discuss specimen requirements for lipid analysis 1.f. Explain the Friedewald calculation 2. Correlate lipid test results with diagnosis, treatment, and prognosis 2.1. by correlating lipid case studies 2.a. Review physiology of triglycerides and cholesterol 2.b. Describe the pathological conditions associated with abnormal lipid values 2.c. Interpret clinical findings in lipidemias 3. Perform renal function tests 3.1. by completing a lab report to document renal function analysis 3.a. Identify lab tests used to evaluate renal function 3.b. Explain methods of analysis of each renal function test 3.c. Identify the reference ranges of the tests used to evaluate renal function 3.d. Discuss specimen requirements for each renal function test 3.e. Explain the creatinine clearance formula 4. Correlate renal test results with diagnosis, treatment, and prognosis 4.1. by correlating renal function case studies 4.a. Review renal physiology 4.b. Describe pathological conditions involving renal function 4.c. Interpret clinical findings in renal disease 5. Analyze blood gas tests 5.1. by completing a blood gas report to document a blood gas analysis 5.a. Identify the tests used to evaluate acid-base balance 5.b. Explain the methods used to measure acid-base balance 5.c. Identify reference ranges of acid-base balance tests 5.d. Discuss specimen requirements for acid-base balance tests 6. Correlate blood gas test results with diagnosis, treatment, and prognosis 6.1. by correlating blood gas case studies 6.a. Review the physiology of acid-base balance 6.b. Describe the pathological conditions associated with abnormal acid-base balance test results 6.c. Interpret acid-base balance results 7. Perform protein tests 7.1. by completing a lab report to document a protein analysis 7.a. Identify clinically significant protein tests Course Outcome Summary - Page 2 of 6
3 7.b. 7.c. 7.d. Explain methods used to measure clinically significant proteins Identify reference ranges for the requested proteins Discuss specimen requirements for requested protein tests 8. Correlate protein test results with diagnosis, treatment, and prognosis 8.1. by correlating protein case studies 8.a. Describe the basic properties of proteins 8.b. Review the metabolism of proteins 8.c. Describe pathological conditions associated with proteins 8.d. Interpret clinical findings of protein tests 9. Perform carbohydrate tests 9.1. by completing a lab report to document carbohydrate analysis 9.a. Identify lab tests used to evaluate carbohydrate levels 9.b. Explain methods used to measure carbohydrate levels 9.c. Identify reference ranges for carbohydrate levels 9.d. Discuss specimen requirements for carbohydrate tests 10. Correlate carbohydrate test results with diagnosis, treatment, and prognosis by correlating carbohydrate case studies 10.a. Review carbohydrate physiology 10.b. Describe pathological conditions related to abnormal carbohydrate levels 10.c. Interpret clinical findings associated with abnormal carbohydrate levels 11. Perform lytes tests by completing an electrolyte report to document electrolyte analysis 11.a. Identify tests included in the basic electrolyte panel 11.b. Explain methods used to measure electrolytes 11.c. Identify the reference ranges for electrolytes 11.d. Discuss specimen requirements 11.e. Explain the concept of osmolality concerning electrolytes 11.f. Explain methods used to measure calcium, magnesium, phosphorous 11.g. Identify the reference ranges for calcium, magnesium, phosphorus 11.h. Discuss specimen requirements of calcium, magnesium, phosphorus 12. Correlate lytes test results with diagnosis, treatment, and prognosis by correlating electrolyte case studies 12.a. Review the physiology of electrolyte balance 12.b. Describe the pathological conditions associated with electrolyte imbalance 12.c. Interpret clinical findings in electrolyte imbalance 12.d. Explain the use of anion gap to correlate results with disease conditions 12.e. Explain the use the osmolal gap results with disease conditions 12.f. Review the physiology of calcium, magnesium, phosphorus 12.g. Describe pathological conditions concerning abnormal calcium, magnesium, phosphorus values 12.h. Interpret abnormal results of calcium, magnesium, phosphorus Course Outcome Summary - Page 3 of 6
4 13. Perform photometric techniques by completing a instrument report to document photometric analysis 13.a. Describe the principles of light 13.b. Describe Beer's law as it relates to spectrophotometric analyses 13.c. Identify the components of a spectrophotometer 13.d. Describe the principle of atomic absorption in the clinical chemistry department<br /> 13.e. Describe the principle of fluorometry in the clinical chemistry department<br /> 13.f. Describe the principle nephelometry in the clinical chemistry department<br /> 13.g. Describe the principle reflectance densitometry in the clinical chemistry department <br /> 13.h. Identify the basic components of each of the following instruments: atomic absorption, fluorometer, nephelometer, reflectance densitometry 13.i. List the application of spectrophotometric analysis in the clinical lab 14. Perform potentiometric techniques by completing an instrument report to document potentiometric analysis 14.a. Explain ion selective electrodes 14.b. Describe the theory utilized in the following electrodes: ph, pco2, po2 14.c. Describe the theory of coulometry 15. Compare and contrast separation techniques by completing a report to document separation techniques 15.a. Describe the theory of electrophoresis 15.b. Identify the components of an electrophoretic system 15.c. Describe the factors affecting electrophoresis 15.d. Summarize total protein electrophoresis 15.e. List the major total protein electrophoretic fractions 15.f. Identify the clinical uses of total protein electrophoresis 15.g. Define the principle of chromatography 16. Evaluate enzyme reactions and testing requirements by completing an enzyme report 16.a. Define enzyme 16.b. Identify the basic properties of enzymes 16.c. List the classes of enzymes and the reaction they catalyze 16.d. Describe the ways in which the following factors may influence enzyme activity: <br />substrate concentration, enzyme concentration, ph, temperature, cofactors, inhibitors<br /> 16.e. Describe enzyme methods used in the lab 17. Perform hepatic/bone tests by completing a lab report to document hepatic/bone analysis 17.a. Identify the types of test(s) used in the laboratory to evaluate liver function 17.b. Explain the laboratory test methods used to measure liver function 17.c. Identify reference intervals for liver function tests 17.d. Discuss specimen requirements for liver function tests 17.e. Identify the types of test(s) used in the laboratory to evaluate bone function Course Outcome Summary - Page 4 of 6
5 17.f. 17.g. 17.h. Explain the laboratory test methods for bone markers Identify reference intervals for bone markers Discuss specimen requirements for liver function tests 18. Correlate hepatic/bone test results with diagnosis, treatment, and prognosis by correlating hepatic/bone case studies 18.a. Review the basic anatomy and physiology of the liver 18.b. Describe the physiology of bile degradation 18.c. Describe the the pathological conditions associated with liver function including: jaundice, kernicterus, physiologic jaundice, hepatitis and cirrhosis<br /> 18.d. Interpret clinical findings in liver disease 18.e. Review the basic physiology of bone formation 18.f. Describe the pathological conditions associated with bone disorders including: osteoporosis, osteomalacia, Paget's disease 19. Perform cardiac markers by completing a lab report to document cardiac marker results 19.a. Identify lab tests used to evaluate cardiac function 19.b. Explain methods used to measure cardiac markers 19.c. Identify reference intervals for cardiac markers 19.d. Discuss specimen requirements 20. Correlate cardiac marker results with diagnosis, treatment, and prognosis by correlating cardiac marker case studies 20.a. Review the anatomy and physiology of the heart 20.b. Identify the source of cardiac markers 20.c. Describe the pathological conditions associated with heart disease including: myocardial infarction and congestive heart disease 20.d. Interpret clinical findings in heart disease 21. Correlate tumor markers with disease states by correlating tumor marker case studies 21.a. Describe the characteristics of tumor markers 21.b. Identify the basic classifications of tumor markers 21.c. Describe the role of tumor markers in the detection and evaluation of cancer 21.d. Describe the pathological conditions associated with abnormal tumor marker levels 21.e. Interpret clinical findings of tumor markers 22. Correlate endocrine test results with diagnosis, treatment, and prognosis by correlating endocrine case studies 22.a. Review the anatomy and physiology of the endocrine system 22.b. Describe the pathological conditions associated with endocrine dysfunction 22.c. Interpret clinical findings in selected endocrine disorders 23. Correlate body fluid test results with diagnosis, treatment, and prognosis Course Outcome Summary - Page 5 of 6
6 23.1. by correlating body fluid test results 23.a. Review the physiology for the following body fluids: amniotic fluid, cerebrospinal fluid, sweat, synovial fluid, serous fluids, and semen 23.b. Review the clinical significance of the following body fluids: amniotic fluid, cerebrospinal fluid, sweat, synovial fluid, serous fluids, and semen 23.c. Identify the types of laboratory tests used to analyze the following body fluids: amniotic fluid, cerebrospinal fluid, sweat, synovial fluid, serous fluids, and semen 23.d. Explain the methods used to analyze: amniotic fluid, cerebrospinal fluid, sweat, synovial fluid, serous fluids, and semen 23.e. Discuss the specimen requirements for: amniotic fluid, cerebrospinal fluid, sweat, synovial fluid, serous fluids, and semen 23.f. Describe the pathological conditions associated with abnormal tests results for the following body fluids: amniotic fluid, cerebrospinal fluid, sweat, synovial fluid, serous fluids, and semen 23.g. Interpret test results for the following body fluids: amniotic fluid, cerebrospinal fluid, sweat, synovial fluid, serous fluids, and semen 24. Evaluate immunoassays by completing a report to document immunoassay(s) 24.a. Describe the basic principle of an antibody antigen reaction 24.b. Describe the principle of enzyme labels including the EMIT methodology 24.c. Describe the principle of fluorescent labels including MEIA and FPIA methodology 24.d. Describe the basic principle of chemiluminescence assays including LIA and ICMA 25. Correlate toxicology (TDM, DAU) test results with diagnosis, treatment, and prognosis by correlating toxicology test results 25.a. Describe the principles of toxicology 25.b. Identify the importance of drug screening in the clinical setting 25.c. Describe the specimen requirements for: drug screens, alcohol levels, carbon monoxide, acetaminophen, salicylate, and lead 25.d. Interpret toxicology test findings Course Outcome Summary - Page 6 of 6
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