Postdoctoral fellow in Queen s University, Department of M icrobiology and
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1 CURRICULUM VITAE Name: Fatemeh Fotouhi Date of birth: Jan. 13, 1966 Place of birth: Iran,Tehran Marital Status: Married, 2 children Address: Influenza unit, Department of Virology, Pasteur Institute of Iran, Tehran, Iran. Phone #: +98 (21) Fax #: +98 (21) Cell#: fotouhi@pasteur.ac.ir, fotouhi44@yahoo.com Education: Postdoctoral fellow in Queen s University, Department of M icrobiology and Immunology, Kingston, Canada Ph.D. in Virology, Tarbiat Modarres University, Tehran, Iran M.Sc. in Virology, Tarbiat Modarres University, Tehran, Iran B.Sc. in Microbiology, Tehran University, Tehran, Iran. Honors and Awards: 2006 First ranked among PhD students, Tarbiat Modarres University Second Prize Winner, Young Researcher, In 4th Razi Festival, Winter 1998, Tehran, 1995 First ranked in M.Sc. Entrance Exam, Tarbiat Modarres University. Employment History: Research assistant, full time. Assisted post-graduated students in their projects Training in Research Department, Minstry of Health M.Sc. student,full time Biology & Chemistry laboratory instructor in State High School (special for top and sagacious students). Established and well-organized up to date laboratories for appropriate practical training of high school students. 1
2 Graduate Students Advised: 2007-present Asghar Abdoli: Construction of recombinant baculovirus encoding HPV-16 L1 gene 2006-Present Ali Ashraf Teimoori : Construction of expression vector containing HPV-16 L1 gene and confirmation of its protein production Mohammad Jazayeri : Evaluation of cross-immunity between HSV-gD1 and HSV-gD2 against HSV 1 and 2 virus infection Marzieh Jamalidoost : Preparation of recombinant glycoprotein G of herpes simplex virus type 2 in prokaryotic system to show HSV-2 Abs Research Experience: Study on project which concerned Role of STAT3 in cancer cells in Canada, Queen s University Ph.D. project which concerned construction of eukaryotic expression vectors (pcdna) and recombinant Baculovirus containing coding region of gd of HSV-2 and evaluation of their efficacy as DNA and subunit vaccines in animal models M.Sc.thesis which concerned adaptation and application of dot immunoassay (DIA) to detect and measure measles virus antibody in human sera, in comparison with that of serum neutralization test. Scientific Skills: Classical Virology Techniques; Insect & eukarutic cell cultures, Virus culture, purification and titration (TCID50, PFU), Serum neutralization test, Hemagglutination, Hemadsorption Molecular Virology Techniques; Gene cloning, Transfection, RNA /DNA Extraction and Purification, clonal selection, PCR/RT-PCR, production of recombinant baculovirus, expression of recombinant protein, blotting, Protein - DNA interaction Immunological Techniques; Immunofluorescence, ELISA, SD S-PAGE Western blotting, DIA, Luciferase assay, EMSA, affinity and ion exchange chromatography, work with experimental animal models 2
3 Bioinformatics; work with some molecular software. Professional Activities: present Member of Iranian Society of Virology Workshops (co-organaizer) : st Workshop on molecular virology"molecular cloning & Expression of recombinant protein " Organized by Tarbiat Modarres University, School of Medical Sciences. Tehran,Iran. Books: "Molecular cloning & Expression of recombinant protein" published by Tarbiat Modarres University, School of Medical Sciences. Tehran I. R. IRAN Publications: 1) Ali Teimoori, Hoorieh Soleimanjahi, Fatemeh Fotouhi, Zahra Meshkat. Isolation and cloning of human papillomavirus 16 L1 gene from Iranian isolate. Saudi Med J 2008; 29 (8): (ISI) 2) Fotouhi Fatemeh., Soleimanjahi Hoorieh*. Roostaee Mohammad Hasan and Dalimi Asl Abdolhossein. "Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea pigs". Iranian Biomedical Journal 2008, 12(2): (ISI) 3) Fotouhi Fatemeh., Soleimanjahi Hoorieh*, Roostaee Mohammad Hasan, and Behzadian Farida. " Enhancement of protective humoral immune responses against herpes simplex virus-2 in DNA-immunized guinea pigs using protein boosting". FEMS Immunol Med Microbiol. 2008, 54: (ISI) 4) Mohammad Jazayeri Farsani, Hoorieh Soleimanjahi*, Fatemeh Fotouhi, and Nafiseh Pakravan. "Comparison of Intramuscular and Footpad Subcutaneous Immunization with DNA Vaccine Encoding HSV- gd2 in Mice. Comparative Immunology, Microbiology and Infectious Diseases, Jun 18. (ISI) 3
4 5) Behzadian F, Sabahi F*, Sadeghizadeh M, Maghsoodi N, Fotouhi F. and Karimi M. "In vitro replication of Hepatitis D Virus using a new construct containing a cdna dimer of HDV genome". Intervirology 2007, 50: (ISI) 6) Mohammad Jazayeri Farsani, Hoorieh Soleimanjahi*, Fatemeh Fotouhi, Taravat Bamdad, Abbas Jamali. "Evaluation of cross immune response in DNA based vaccinated mice against HSV-1 and HSV-2". Gene Therapy & Molecular Biology, 2007, (11): (ISI) 7) H. Soleimanjahi*, F. Fotouhi, T. Bamdad. MH. Roostaee, "Evaluation of HI antibodies against rubella virus to determine the immune status, regarding the mass campaign vaccination in Tehran, Iran", Iranian Journal of Virology, 2007, (1), ) Marzieh Jamalidoost, Hoorieh Soleimanjahi*, Fatemeh Fotouhi and Zahra Meshkat "Amplification and Cloning of Herpes Simplex Virus Type 2 Glycoprotein- G from an Iranian Isolate". Pakistan Journal of Biological science. 2007,10(6): (ISI) 9) Soleimanjahi H*, Bamdad T, Fotouhi F, Roostaee MH, Faghihzadeh S." Prevalence of HI antibody titer against rubella virus to determine the effect of mass vaccination in Tehran". J Clin Virol. 2005, 34(2): (ISI) 10) Fotouhi Chahooki, F., Roostaee*, M. H., Soleimanjahi. H and Haqshenash, G. R. "Construction of an Eukaryotic Expression Vector Encoding Herpes Simplex Virus Type 2 Glycoprotein D (gd2) and In Vitro Expression of the Desired protein. Arch.Razi. 2005(59), ) Fotouhi F., Roustaee* M.H., Khabiri A.R., Soleimanjahi H., Malekane M., and F.Sabahi. "Application of Dot Immunoassay for Detection and Titration of Measles Virus Antibodies". Med.J. of the IR.IRAN 2001, 15(1): Abstracts: 1) Fotouhi F.,Roustaee M.H.,Khabiri A.R., Soleimanjahi H., MalekaneM., Application of Dot Immunobinding assay using Measles antigens for Detection and Titration of Antibodies. 4th Biochem. Cong. Spring 1996.Tehran,IRAN. 2) Roustaee M.H., Soleimanjahi H. Bamdad T. Fotouhi F. Techniques for measuring Rubeola antibodies,pakistan SOCITY FOR MICROBIOLOGY SIBC-PSM
5 3) Fotouhi F., Roustaee M.H., Haqshenas G.R.,and M.Karimi. Construction of expression vector pcdna3 encoding glycoprotein D of Herpes Simplex Virus type 2(HSV2).The second congress on virology, Winter 2004.Tehran, IRAN. 4) Fotouhi. F., Roustaee. M.H., Soleimanjahi. H. and Haqshenas.G.R. Expression of the Glycoprotein D of Herpes Simplex Virus Type-2 in Insect Cells.,HUPO 3rd Annual World Congress. October 2004.Beijing,China. 5) Jamalidoust.M., Fotouhi.F. and Roustaee.M.H. The preparation of protein G-2 of Herpes Simplex Virus Type-2 (HSV-2) in Prokaryotic System to Show Type Specific Antibody Against This Virus., HUPO 3rd Annual World Congress. October 2004.Beijing,China. 6) Fotouhi.F., Soleimanjahi.H. and Roustaee.M.H. Construction of an expression vector containing HSV-1&2 glycoprotein D genes to express in mammalian cell lines.the 9th Congress on Obsterics and Gynaecology.2004.Tehran, Iran. 7) Jamalidoust.M., Fotouhi.F. and Roustaee.M.H. The preparation of Herpes Simplex Virus type-2 glycoprotein G to identify pregnant mothers that afflicated of herpes simplex virus type-2. The 9th Congress on Obsterics and Gynaecology Tehran, Iran. 8) Jamalidoust M, Fotouhi F., Soleimanjahi H., Roosaee MH. Development of type specific based glycoprotein G assay to identify of HSV-2 infection. April 2005,15th ECCMID, Copenhagen,Denmark. 9) Fotouhi F., Roostaee MH., Soleimanjahi H. Expression and purification of herpes simplex virus type 2 glycoprotein D in insect cells using the baculovirus exprresion system. April 2005,15th ECCMID, Copenhagen, Denmark. 10) Soleimanjahi H, Bamdad T, Fotouhi F., Roosaee MH, Siadat SD. Seroprevalance of rubella antibodies among young population in Tehran, Iran. April 2005,15th ECCMID, Copenhagen, Denmark. 11) Fotouhi F., Roustaee MH, Soleimanjahi H. Modification of primary and recurrent genital herpes in guinea pig by DNA immunization. (Oral) The 4th national biotechnology congress of Islamic republic of Iran Agu (2005), Kerman. 12) Fotouhi F., Soleimanjahi H, Roostaee MH., and Dalimi A. Preparation of the herpes simplex virus type 2 glycoprotein D subunit and DNA vaccines and evaluation of their efficacy in guinea pigs. April 2007,17th ECCMID, Munich, Germany. 5
6 13) Fotouhi F., Soleimanjahi H, Roostaee MH., and Dalimi A. DNA priming-protein boosting using HSV-gD2 induces higher humoral responses compared to DNA immunization. ( Oral) The 4th Iranian congress on Virology, Spring 2007.Tehran,IRAN. 14) Teimoori A, Soleimanjahi H, Fotouhi F, Meshkat Z, Khansarinejad B. Isolation and amplification of HPV-16 L1 gene from paraffin embedded tissue samples. (Oral) The 4th Iranian congress on Virology, Tehran, Iran. Jan ) Soleimanjahi H, Fotouhi F. Administration of DNA and protein vaccines containing gd genes of HSV1 and 2. April 2008,18th ECCMID, Barcelona, Spain. 16) Jazayeri M, Soleimanjahi H, Fotouhi F., Evaluation of inoculation method of DNA vaccine containing gd against HSV-2.9th congress of cancer association.tehran, Iran ) Abdoli A., Soleimanjahi H., Fotouhi chahooki F., Teimoori A., Fazeli M., Farzaneh pour F.,. Shenagari M, Construction of a recombinant bacmid containing papillomavirus type16-l1 gene. 18th ECCMID,2008. Research Interest: DNA and Subunit Vaccines Baculovirus expression system Adenoviral expression system, Insect & eukaryotic cell cultures, Oncolytic viruses and viral oncoproteins, Bio-nonoparticles like VLPs Referees: 1) Professor A. Dalimi Asl, Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University,Tehran, Iran Dalimi@modares.ac.ir 2) Professor M.Tavassoti Kheiri, Influenza Unit, Pasteur Institute of Iran, Tehran, Iran mtkheiri@pasteure.ac.ir 3) Professor H. Soleimanjahi, Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University,Tehran, Iran Soleim_h@modares.ac.ir 4) Professor T. Mokhtari Azad, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran mokhtari@sina.tums.ac.ir 6
CURRICULUM VITAE M.Sc. in Medical Virology, Tarbiat Modarres University, Tehran, Iran.
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