Clinical ICSI in the horse:
|
|
- Eunice Byrd
- 5 years ago
- Views:
Transcription
1 Clinical ICSI in the horse: differences and similarities to human in an in vitro maturation-based system Katrin Hinrichs College of Veterinary Medicine & Biomedical Sciences Texas A&M University
2
3 Standard In Vitro Fertilization Low fertilization rates, not repeatable Major barrier: Sperm fail to penetrate oocyte zona pellucida
4 Equine ICSI
5
6 Horse Progesterone OV LH Luteal Follicular Luteal
7 Chorionic/ Placental hormone production Progestins
8 Chorionic/ Placental hormone production Progestins
9 Chorionic/ Placental hormone production Progestins
10 Chorionic/ Placental hormone production 2 o CLs Progestins
11 Chorionic/ Placental hormone production 2 o CLs Progestins
12
13 Choi et al., 2015 CDX2 GATA6
14 Blastocyst biopsy for PGD
15 Day-7 blastocyst recovered from uterus
16
17 7-d blastocyst (403 µm) After biopsy After vitrification 24 h culture Choi et al., 2011
18 Why Clinical ICSI?
19 Subfertile mare Mare cannot provide embryo for transfer Endometritis/Pyometra Uterine adhesions Cervical tear Oviductal blockage? Idiopathic
20 Post Mortem
21
22 Low semen stores Only semen available is frozen, low quality or numbers Deceased stallion Aged stallion no longer fertile
23 Oocyte Recovery
24
25
26
27
28 Recovery from Stimulated PRE-OVULATORY Follicle
29
30 Management Monitor follicle growth via repeated transrectal ultrasonography Predict first day of projected responsiveness from size, shape, wall thickness Administer hcg or deslorelin Aspirate follicle 24 to 35 h later
31 Why not superovulate? Superovulation not repeatable in the horse Equine FSH can be effective - 3 follicles Ovary hard to manipulate if >1-2 large follicles present
32
33 Trans-vaginal Ultrasound-guided Follicle Aspiration (TVA)
34
35
36 Dominant Stimulated Follicle 70 85% recovery
37 24 h after hcg MI 35 h after hcg - MII Bezard et al., 1997
38 We designate these in vivo matured oocytes Culture (no hormones needed) until ~36-42 h after donor received hcg Recover at 24 h, culture 12 h Recover at 35 h, culture 1 h
39 ~ 40% blastocyst rate Jacobson et al., 2010
40 Drawbacks to Aspirating DSF: Monitoring of estrus and follicle activity in the mare Timing of gonadotropin stimulation in relation to follicle maturity Timing of aspiration at a set point after administration of gonadotropin stimulation Inability to schedule these procedures beforehand, due to variations in follicle growth Recovery at most only one maybe two oocytes Must perform ICSI at a set time after gonadotropin stimulation
41 Recovery from IMMATURE Follicles
42
43 Immature Follicles: Present in the ovary throughout the year
44
45 Immature Follicles: Growing follicle? Atretic follicle?
46 Work with Oocytes from Ovaries recovered Post Mortem
47
48 Expanded Compact Expanded-cumulus oocytes (Ex) had a higher rate of maturation in vitro
49 Ex oocytes came from atretic follicles Oocytes from atretic follicles had a higher maturation rate
50 Developmental competence?
51 Immature Oocyte Cumulus Type and Maturation Duration (post-mortem tissue) (DMEM/F-12, 5% 0 2, 5% CO 2, 90% N 2 ) Cumulus Type Mat time n % Cleav % Blast Cp 24 h 30 h 36 h a 35 b 38 b Ex 24 h 30 h 36 h Hinrichs et al., 2005
52 Human: SN Human: NSN Monti et al., 2017
53
54 LH
55
56
57
58
59 Early results with TVA of Immature Follicles Many laboratories LOW Recovery Rate: 20% recovery rate = 2 to 6 follicles/session 1 to 2 immature oocytes /session
60 Hawley et al., 1995
61 Bovine Bovine Equine Equine Hawley et al., 1995
62 Hawley et al., 1995
63 Aspirate Scrape while emptying Fill Aspirate Scrape while emptying Fill Aspirate Scrape while emptying Fill. (6 times) Hawley et al., 1995
64 2017 Research Data TAMU 229 research transvaginal aspirations Average 71.1% oocyte recovery Average 10 oocytes per mare
65 Advantages to aspirating Immature Follicles: No hormonal stimulation needed No monitoring of estrus or follicle activity Can do on fixed schedule, e.g. once every two weeks Large number of follicles; more oocytes recovered
66 30 h maturation
67 Trials to delay onset of maturation Follicular fluid Follicle wall 6-DMAP Cycloheximide Roscovitine Butyrolactone Hinrichs et al., 1995; Alm and Hinrichs, 1996; Hinrichs et al., 2002; Franz et al., 2003
68
69 Overnight holding before maturation Immature oocytes placed in modified M199 Held overnight at ROOM TEMPERATURE No detrimental effect on Maturation rate Held: 65% Control: 68% No detrimental effect on Blastocyst rate Held: 34% Control: 25% Choi et al., 2006
70 Shipped oocytes
71 Oocyte shipment to the ICSI Lab: Room Temperature vs. Cold Treatment No. oocytes No. mature oocytes/icsi (%) No. Blastocysts (% of injected) Cold (5-10 C) (23.1) a 5 (16.7) a Room Temperature (21-24 C) (37.3) b 20 (40.8) b
72 ICSI Case Load Texas A&M
73 Equal quality blastocyts?
74 Clinical ICSI results 2016 In vivo matured oocytes vs. In vitro matured (IVM) oocytes Oocyte type Blastocyst rate Pregnancy rate Ongoing pregnancy (heartbeat) In vivo 47/123 (38%) 26/36 (72%) 24/36 (67%) IVM 351/1210 (29%) 166/234 (71%) 146/234 (62%)
75 Oocyte maturation medium M199/Earle s salts 10% fetal bovine serum 5 mu/ml bovine FSH
76
77 Acknowledgements Members of the Equine Embryo Laboratory Texas A&M University
78 Members of the Section of Theriogenology Texas A&M Participating ET Centers The Link Equine Research Endowment Fund, Texas A&M University
EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM
COLLEGE OF VETERINARY MEDICINE & BIOMEDICAL SCIENCES Department of Large Animal Clinical Sciences 4475 TAMU EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM Section of Theriogenology Veterinary Medical
More informationEQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM
COLLEGE OF VETERINARY MEDICINE & BIOMEDICAL SCIENCES Department of Large Animal Clinical Sciences 4475 TAMU January 2016 b EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM Section of Theriogenology & the
More informationProceedings of the Society for Theriogenology Annual Conference 2014
www.ivis.org Proceedings of the Society for Theriogenology Annual Conference 2014 Aug. 6-9, 2014 Portland, OR, USA Next SFT Meeting: Aug. 5-8, 2015 San Antonio, TX, USA Reprinted in the IVIS website with
More informationWEATHERFORD EQUINE BREEDING CENTER 6375 MINERAL WELLS HWY WEATHERFORD, TX PHONE: FAX:
WEATHERFORD EQUINE BREEDING CENTER 6375 MINERAL WELLS HWY WEATHERFORD, TX 76088 PHONE: 817-594-9181 FAX: 817-594-9183 Oocyte Services Weatherford Equine (WE) is very experienced in providing for all the
More informationEQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM. Equine Embryo Laboratory College of Veterinary Medicine & Biomedical Sciences Texas A&M University
COLLEGE OF VETERINARY MEDICINE & BIOMEDICAL SCIENCES Department of Large Animal Clinical Sciences 4475 TAMU January 2017 EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM Equine Embryo Laboratory College
More informationWEATHERFORD EQUINE BREEDING CENTER 6375 MINERAL WELLS HWY WEATHERFORD, TX PHONE: FAX:
Oocyte Services Weatherford Equine (WE) is very experienced in providing for all the needs associated with collecting oocytes from your mare to undergo ICSI fertilization. Overview of the Process for Utilizing
More informationIn Vitro Production of Equine Embryos: State of the Art
Reprod Dom Anim 45 (Suppl. 2), 3 8 (2010); doi: 10.1111/j.1439-0531.2010.01624.x ISSN 0936-6768 In Vitro Production of Equine Embryos: State of the Art K Hinrichs Departments of Veterinary Physiology and
More informationProceedings of the 12th International Congress of the World Equine Veterinary Association WEVA
www.ivis.org Proceedings of the 12th International Congress of the World Equine Veterinary Association WEVA November 2-5, 2011 Hyderabad, India Reprinted in IVIS with the Permission of WEVA Organizers
More informationTEXAS A&M UNIVERSITY College of Veterinary Medicine and Biomedical Sciences Department of Veterinary Physiology & Pharmacology
TEXAS A&M UNIVERSITY College of Veterinary Medicine and Biomedical Sciences Department of Veterinary Physiology & Pharmacology Fee Schedule for Equine Embryo Laboratory (Dr. Hinrichs) Page 1 of 2 Updated
More informationFemale Reproductive System. Lesson 10
Female Reproductive System Lesson 10 Learning Goals 1. What are the five hormones involved in the female reproductive system? 2. Understand the four phases of the menstrual cycle. Human Reproductive System
More informationIndex. Note: Page numbers of article titles are in boldface type.
Note: Page numbers of article titles are in boldface type. A N-Acetylcysteine in endometritis management, 506 Acupuncture in endometritis management, 508 Anatomic defects correction of in persistent post-breeding
More informationNew Assisted Reproductive Techniques for Horses. Dirk K. Vanderwall, DVM, PhD, Dipl. ACT
New Assisted Reproductive Techniques for Horses Dirk K. Vanderwall, DVM, PhD, Dipl. ACT Northwest Equine Reproduction Laboratory Department of Animal and Veterinary Science Center for Reproductive Biology
More informationUse of in vitro maturation for fertility preservation
Use of in vitro maturation for fertility preservation G. Arroyo Servei de Medicina de la Reproducció Departament d Obstetrícia, Ginecologia i Reproducció INSTITUT UNIVERSITARI DEXEUS MEDICAL STRATEGY TO
More informationBiology of fertility control. Higher Human Biology
Biology of fertility control Higher Human Biology Learning Intention Compare fertile periods in females and males What is infertility? Infertility is the inability of a sexually active, non-contracepting
More information2018 Oocyte Recovery and ICSI
2018 Oocyte Recovery and ICSI Equine Medical Services is one of the world s leading producers of in vitro produced embryos. While standard IVF techniques are ineffective with horses, ICSI (Intracytoplasmic
More informationIn vitro production of equine embryos and genetic testing
In Vitro Fertilization (FIV) In Vitro Production of Equine Embryos and Preimplantation Genetic Testing Oocytes from or slaughterhouse ovaries Sperm sample Carolina Herrera, M Sc. Clinic for Reproductive
More informationPreliminary Scientific Program
Preliminary Scientific Program Thursday, 27th September 2018 Pre-conference sessions Practical parallel sessions Veterinary Teaching Hospital, Faculty of Veterinary Medicine 09:00-18:30 1 Equine oocyte
More information2018 Oocyte Recovery and ICSI
2018 Oocyte Recovery and ICSI Equine Medical Services is one of the world s leading producers of in vitro produced embryos. While standard IVF techniques are ineffective with horses, ICSI (Intracytoplasmic
More informationEffect of potential oocyte transport protocols on blastocyst rates after intracytoplasmic sperm injection in the horse
bs_bs_banner Effect of potential oocyte transport protocols on blastocyst rates after intracytoplasmic sperm injection in the horse R. FOSS*, H. ORTIS and K. HINRICHS Equine Medical Services, Inc., Missouri,
More informationI. ART PROCEDURES. A. In Vitro Fertilization (IVF)
DFW Fertility Associates ASSISTED REPRODUCTIVE TECHNOLOGY (ART) Welcome to DFW Fertility Associates/ Presbyterian-Harris Methodist Hospital ARTS program. This document provides an overview of treatment
More informationLOW RESPONDERS. Poor Ovarian Response, Por
LOW RESPONDERS Poor Ovarian Response, Por Patients with a low number of retrieved oocytes despite adequate ovarian stimulation during fertility treatment. Diagnosis Female About Low responders In patients
More informationAssisted Reproduction. By Dr. Afraa Mahjoob Al-Naddawi
Assisted Reproduction By Dr. Afraa Mahjoob Al-Naddawi Learning Objectives: By the end of this lecture, you will be able to: 1) Define assisted reproductive techniques (ART). 2) List indications for various
More information10.7 The Reproductive Hormones
10.7 The Reproductive Hormones December 10, 2013. Website survey?? QUESTION: Who is more complicated: men or women? The Female Reproductive System ovaries: produce gametes (eggs) produce estrogen (steroid
More informationChapter 14 Reproduction Review Assignment
Date: Mark: _/45 Chapter 14 Reproduction Review Assignment Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Use the diagram above to answer the next question.
More informationFemale reproductive cycle: A Comprehensive Review Rachel Ledden Paper for Bachelors in Science January 20, 2018
Running head: 1 Female reproductive cycle: A Comprehensive Review Rachel Ledden Paper for Bachelors in Science January 20, 2018 Female reproductive cycle: A Comprehensive Review 2 The reproductive cycle
More informationPricelist. Consultations & other investigations
Pricelist Please note as part of pre-treatment consultations, you may be required to have blood tests performed, the costs of which are not included in the treatment cycles. These costs are outlined below.
More informationEMBRYO TRANSFER IN HORSES
Vet Times The website for the veterinary profession https://www.vettimes.co.uk EMBRYO TRANSFER IN HORSES Author : Jonathan F Pycock, James R Crabtree Categories : Vets Date : March 14, 2011 Jonathan F
More informationAnimal Reproduction Science 98 (2007) 39 55
Animal Reproduction Science 98 (2007) 39 55 Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation
More informationFAILED OOCYTE MATURATION. A Fekih, N Farah, D Chardonnens, F Urner, D De Ziegler, PG Bianchi, P Mock, A Campana, H Lucas
FAILED OOCYTE MATURATION A Fekih, N Farah, D Chardonnens, F Urner, D De Ziegler, PG Bianchi, P Mock, A Campana, H Lucas INTRODUCTION In our laboratory,we perform (X) IVF and (Y) ICSI per year with a success
More informationApplication of Ovum Pick-Up, Intracytoplasmic Sperm Injection and Embryo Culture in Equine Practice
REPRODUCTIO Application of Ovum Pick-Up, Intracytoplasmic Sperm Injection and Embryo Culture in Equine Practice Silvia Colleoni, PhD; Sandro Barbacini, DVM*; Denis ecchi, DVM, Diplomate ECAR; Roberto Duchi,
More informationCauses of Infertility and Treatment Options
Causes of Infertility and Treatment Options Dr Mrs.Kiran D. Sekhar Former vice President-FOGSI Former Chairperson- Genetics and Foetal medicine-fogsi Founder and Medical Director-Kiran Infertility centre
More informationby Bergero and Cynthia Préfontaine photos by Shary B. Akers Introduction
by Bergero and Cynthia Préfontaine photos by Shary B. Akers Introduction The embryo transfer (ET= embryo transfer) is a breeding method or reproductive technology, where an embryo is flushed from a donor
More informationReproductive Endocrinology & Infertility Glossary
Reproductive Endocrinology & Infertility Glossary The following is a glossary of terms you may hear during your association with the University of Mississippi Health Care's reproductive endocrinology and
More informationInfertility. Review and Update Clifford C. Hayslip MD Intrauterine Inseminations
Infertility Review and Update Clifford C. Hayslip MD Intrauterine Inseminations Beneficial effects of IUI not consistently documented in studies No deleterious effects on fertility 3-4 cycles of IUI should
More informationINDICATIONS OF IVF/ICSI
PROCESS OF IVF/ICSI INDICATIONS OF IVF/ICSI IVF is most clearly indicated when infertility results from one or more causes having no other effective treatment; Tubal disease. In women with blocked fallopian
More informationThe Human Menstrual Cycle
The Human Menstrual Cycle Name: The female human s menstrual cycle is broken into two phases: the Follicular Phase and the Luteal Phase. These two phases are separated by an event called ovulation. (1)
More informationIVM in PCOS patients. Introduction (1) Introduction (2) Michael Grynberg René Frydman
IVM in PCOS patients Michael Grynberg René Frydman Department of Obstetrics and Gynecology A. Beclere Hospital, Clamart, France Maribor, Slovenia, 27-28 February 2009 Introduction (1) IVM could be a major
More informationReproduction and Development. Female Reproductive System
Reproduction and Development Female Reproductive System Outcomes 5. Identify the structures in the human female reproductive system and describe their functions. Ovaries, Fallopian tubes, Uterus, Endometrium,
More informationInformation Booklet. Exploring the causes of infertility and treatment options.
Information Booklet Exploring the causes of infertility and treatment options www.ptafertility.co.za info@ptafertility.co.za +27 12 998 8854 Faith is taking the first step even if you don t see the whole
More informationWeb Activity: Simulation Structures of the Female Reproductive System
differentiate. The epididymis is a coiled tube found along the outer edge of the testis where the sperm mature. 3. Testosterone is a male sex hormone produced in the interstitial cells of the testes. It
More informationFertility 101. About SCRC. A Primary Care Approach to Diagnosing and Treating Infertility. Definition of Infertility. Dr.
Dr. Shahin Ghadir A Primary Care Approach to Diagnosing and Treating Infertility St. Charles Bend Grand Rounds November 30, 2018 I have no conflicts of interest to disclose. + About SCRC State-of-the-art
More informationStudy on Several Factors Involved in IVF-ET of Human Beings
Study on Several Factors Involved in IVF-ET of Human Beings Lei X 1, Zhuoran W 1, Bin L 1, Huiming L 1, Hongxiu Z 1, Yajuan Z 1, Yingbo Q 1, Guixue Z 2 1 The First Clinical College of Harbin Medical University,
More informationPatient Price List. t: e: w:
Patient Price List t: 0333 015 9774 e: enquires@ivi.uk w: www.ivi.uk fertility treatments Pre Treatment Medical Consultation 250 Nurse Planning 200 Baseline ultrasound scan of uterus and ovaries included
More informationVM 744 THERIOGENOLOGY Spring 2013
VM 744 THERIOGENOLOGY Spring 2013 Credits-3 Coordinator: Patrick M., DVM, PhD, Diplomate ACT Department of Clinical Sciences Office: Equine Reproduction Laboratory Phone: 491-8626 email: pmccue@colostate.edu
More informationPage 1. A wide variety of ovarian abnormalities are encountered in clinical practice
A wide variety of ovarian abnormalities are encountered in clinical practice Common Problems Anovulatory follicles Persistent anovulatory follicles Hemorrhagic/Luteinized follicles Persistent corpus luteum
More informationPuerto Rico Fertility Center
Puerto Rico Fertility Center General Information of the In-Vitro Fertilization Program Dr. Pedro J. Beauchamp First test-tube baby IN PUERTO RICO Dr. Pedro Beauchamp with Adlin Román in his arms. Paseo
More informationResearch Article Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries
SAGE-Hindawi Access to Research International Volume 2010, Article ID 102591, 4 pages doi:10.4061/2010/102591 Research Article Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries F.
More informationNew assisted reproductive technologies applied to the horse industry: successes and limitations
Anim. Reprod., v.5, n.3/4, p.67-82, Jul./Dec. 2008 New assisted reproductive technologies applied to the horse industry: successes and limitations F.C. Landim-Alvarenga 1, C.B. Fernandes, L.G. Devito,
More informationSmall Ruminant Reproductive Management Workshop
Small Ruminant Reproductive Management Workshop Animal Nutrition and Physiology Center, North Dakota State University Sponsors: American Sheep and Goat Center, North Dakota State University, University
More informationIs it the seed or the soil? Arthur Leader, MD, FRCSC
The Physiological Limits of Ovarian Stimulation Is it the seed or the soil? Arthur Leader, MD, FRCSC Objectives 1. To consider how ovarian stimulation protocols work in IVF 2. To review the key events
More informationLecture 14: Conception, Fertility, Early Fetal Loss. provera. Depo-provera. Early Fetal Loss. Implanon. Norplant. Nuva Ring.
Lecture 14: Conception, Fertility, Early Fetal Loss Birth Control (cont.) Conception What Influences Probability of Conception? Early Fetal Loss Infertility Fertility Enhancement Depo-provera provera Injectable
More informationOocyte maturation. A.Trounson 1 ' 3, C.Anderiesz 1, G.MJones 1, A.Kausche 1, N.Lolatgis 2 and C.Wood 2
A.Trounson 1 ' 3, C.Anderiesz 1, G.MJones 1, A.Kausche 1, N.Lolatgis 2 and C.Wood 2 Centre for Early Human Development, Institute of Reproduction and Development, Monash University, Monash Medical Centre,
More informationAP Biology Ch ANIMAL REPRODUCTION. Using only what you already know (you cannot look up anything) complete the chart below.
AP Biology Ch. 46 - ANIMAL REPRODUCTION Using only what you already know (you cannot look up anything) complete the chart below. I. Overview of Animal Reproduction A. Both asexual and sexual reproduction
More informationAssisted reproductive techniques in mares
Received: 01 March 2018 Revised: 13 May 2018 Accepted: 16 May 2018 DOI: 10.1111/rda.13259 PLENARY Assisted reproductive techniques in mares Katrin Hinrichs Department of Veterinary Physiology and Pharmacology,
More informationChapter 14 The Reproductive System
Biology 12 Name: Reproductive System Per: Date: Chapter 14 The Reproductive System Complete using BC Biology 12, page 436-467 14. 1 Male Reproductive System pages 440-443 1. Distinguish between gametes
More informationIN VITRO FERTILISATION (IVF)
IN VITRO FERTILISATION (IVF) Pre Treatment - first cycle 785 Medical Consultation 225 Nurse Planning 235 Baseline ultrasound scan of uterus and ovaries HIV, Hep B antibodies, Hep B antigen, Hep C blood
More informationThe reproductive lifespan
The reproductive lifespan Reproductive potential Ovarian cycles Pregnancy Lactation Male Female Puberty Menopause Age Menstruation is an external indicator of ovarian events controlled by the hypothalamicpituitary
More informationOutline. Male Reproductive System Testes and Sperm Hormonal Regulation
Outline Male Reproductive System Testes and Sperm Hormonal Regulation Female Reproductive System Genital Tract Hormonal Levels Uterine Cycle Fertilization and Pregnancy Control of Reproduction Infertility
More informationInfluence ovarian stimulation on oocyte and embryo quality. Prof.Dr. Bart CJM Fauser
Influence ovarian stimulation on oocyte and embryo quality Prof.Dr. Bart CJM Fauser How to balance too much vs too little? Lecture Outline Context ovarian stimulation Impact ovarian stimulation on oocyte
More informationReproductive Endocrinology. Isabel Hwang Department of Physiology Faculty of Medicine University of Hong Kong Hong Kong May2007
Reproductive Endocrinology Isabel Hwang Department of Physiology Faculty of Medicine University of Hong Kong Hong Kong May2007 isabelss@hkucc.hku.hk A 3-hormone chain of command controls reproduction with
More information1 st International Symposium on Bison Health
Faculty of Veterinary Medicine 1 st International Symposium on Bison Health Assisted Reproductive Technologies in Bison Robert McCorkell June 26, 2015 Artificial Insemination Frozen semen Estrus synchronization
More informationSelect Breeders Services
Select Breeders Services MANAGEMENT OF MARES FOR FROZEN SEMEN INSEMINATION Verify that the mare is a suitable candidate for AI with frozen semen by performing a routine reproductive exam that may include
More informationInfertility treatment
In the name of God Infertility treatment Treatment options The optimal treatment is one that provide an acceptable success rate, has minimal risk and is costeffective. The treatment options are: 1- Ovulation
More informationSperm production. Sperm production. Meiosis. Mitosis. The cells of Leydig in testes secrete
Sperm production Ductus deferens Epididymis The cells of Leydig in testes secrete Seminiferous testosterone (T) tubules T secreted at puberty produces 2 o sex characteristics, spermatogenesis, & maintain
More informationSperm production. Sperm production. Controlling sperm production. Meiosis. Mitosis. The cells of Leydig in testes secrete
Ductus deferens Sperm production Epididymis The cells of Leydig in testes secrete Seminiferous testosterone (T) tubules T secreted at puberty produces 2 o sex characteristics, spermatogenesis, & maintain
More informationBiology of gender Sex chromosomes determine gonadal sex (testis-determining factor)
Indifferent ducts of embryo Biology of gender Sex chromosomes determine gonadal sex (testis-determining factor) Y chromosome present Y chromosome absent Phenotypic sex is depends on development of external
More informationBiology of gender Sex chromosomes determine gonadal sex (testis-determining factor)
Indifferent ducts of embryo Y chromosome present Y chromosome absent Male Female penis ovary uterus vagina testis Biology of gender Sex chromosomes determine gonadal sex (testis-determining factor) Phenotypic
More informationIn vitro maturation of human oocytes for assisted reproduction
MODERN TRENDS Edward E. Wallach, M.D. Associate Editor In vitro maturation of human oocytes for assisted reproduction Marcus W. Jurema, M.D., and Daniela Nogueira, Ph.D. Department of Obstetrics and Gynecology,
More informationINFERTILITY. Services - Part 2
INFERTILITY Services - Part 2 1 Imprint Published in January, 2013 By Victory A.R.T. Laboratory Phils, Inc. These articles were compiled by in hopes of helping bring into light infertility questions and
More informationAnS 214 SI Multiple Choice Set 4 Weeks 10/14-10/23
AnS 214 SI Multiple Choice Set 4 Weeks 10/14-10/23 The following multiple choice questions pertain to material covered in the last two weeks' lecture sets. Answering the following questions will aid your
More informationNeil Goodman, MD, FACE
Initial Workup of Infertile Couple: Female Neil Goodman, MD, FACE Professor of Medicine Voluntary Faculty University of Miami Miller School of Medicine Scope of Infertility in the United States Affects
More informationThe Science of your Cycle
The Science of your Cycle Day 3: Get to know your cycle (Part I) with Jennifer Aldoretta Cofounder & CEO of Groove Today s goals Learn how your hormones work together to create the changes that happen
More informationTestes (male gonads) -Produce sperm -Produce sex hormones -Found in a sac called the scrotum -Suspended outside of the body cavity for temperature
REPRODUCTION Testes (male gonads) -Produce sperm -Produce sex hormones -Found in a sac called the scrotum -Suspended outside of the body cavity for temperature reduction -Testes wall made of fibrous connective
More informationAdvanced Assisted Reproductive Technologies
Advanced Assisted Reproductive Technologies 體外受孕中心 IVF Centre IVF CENTRE at Hong Kong Sanatorium & Hospital The IVF Centre at Hong Kong Sanatorium & Hospital has been offering excellent patient care and
More informationOvarian follicular dynamics and superovulation in cattle
Ovarian follicular dynamics and superovulation in cattle John P Kastelic Professor of Theriogenology Head, Department of Production Animal Health University of Calgary Calgary, Alberta, Canada Factors
More informationConsultations and Assessment Fertility Specialist consultation 180 Ultrasound scan of uterus and ovaries 100 AMH measurement 80 Semen analysis 100
We hope this price list will help you assess the cost of your consultations, investigations and treatment. It provides information about what is included in the cost and how we make a refund if your treatment
More informationChapter 36 Active Reading Guide Reproduction and Development
Name: AP Biology Mr. Croft Chapter 36 Active Reading Guide Reproduction and Development Section 1 1. Distinguish between sexual reproduction and asexual reproduction. 2. Which form of reproduction: a.
More informationIn Vitro Fertilization in Clomiphene-Resistant Women with Polycystic Ovary Syndrome
Original Article Effect of Laparoscopic Ovarian Drilling on Outcomes of In Vitro Fertilization in Clomiphene-Resistant Women with Polycystic Ovary Syndrome Maryam Eftekhar, M.D. 1, Razieh Deghani Firoozabadi,
More informationDr. Julio Giordano. Ovulation. Follicle. Corpus Luteum. GnRH
Dr. Julio Giordano Follicle Corpus Luteum LH FSH E2 Hypothalamic hormones Gonadotropin releasing hormone () Hormone Concentration CL LH (ng/ml) 12 10 8 6 4 2 LH Response Cows Treated with 28 h (22-36)
More information1. Be able to characterize the menstrual cycle from the perspective of the ovary a. Follicular phase b. Luteal phase
Human Sexuality Exam II Review Material Gametogenesis: Oogenesis 1. Be able to characterize the menstrual cycle from the perspective of the ovary a. Follicular phase b. Luteal phase 2. Know the relative
More informationEMBRYO TRANSFER ANIMAL SCIENCE 8818-B INTRODUCTION
ANIMAL SCIENCE 8818-B EMBRYO TRANSFER INTRODUCTION Embryo transfer* is a process by which an embryo is collected from a donor female and then transferred into a recipient female where the embryo completes
More informationAnimal Science 434! Tonic and Preovulatory Surge of GnRH! Tonic and Preovulatory Surge of GnRH! Lecture 11: The Follicular Phase of the Estrous Cycle!
Tonic and Preovulatory Surge of GnRH! Animal Science 434! Lecture 11: The Follicular Phase of the Estrous Cycle!! (-)! Hypothalamus! GnRH! Estradiol! (-)! Tonic and Preovulatory Surge of GnRH! Anterior!
More informationKisspeptin: A Potential Factor for Unexplained Infertility and Impaired Embryo Implantation
Original Article Kisspeptin: A Potential Factor for Unexplained Infertility and Impaired Embryo Implantation Aaida Mumtaz, MBBS. 1#, Aqsa Khalid, MBBS. 1#, Zehra Jamil, M.Phil. 2, Syeda Sadia Fatima, Ph.D.
More informationUnderstanding eggs, sperm and embryos. Marta Jansa Perez Wolfson Fertility Centre
Understanding eggs, sperm and embryos Marta Jansa Perez Wolfson Fertility Centre What does embryology involve? Aims of the embryology laboratory Creation of a large number of embryos and supporting their
More informationREPRODUCTION & GENETICS. Hormones
REPRODUCTION & GENETICS Hormones http://www.youtube.com/watch?v=np0wfu_mgzo Objectives 2 Define what hormones are; Compare and contrast the male and female hormones; Explain what each hormone in the mail
More informationPrice List. Valid from 1 st April 2017
Price List Valid from 1 st April 2017 Consultations & Assessments Consultations & Tests Medical Consultation 200 Nurse Planning Consultation (includes ultrasound scan) 230 Consultation with Counsellor
More informationChapter 28: REPRODUCTIVE SYSTEM: MALE
Chapter 28: REPRODUCTIVE SYSTEM: MALE I. FUNCTIONAL ANATOMY (Fig. 28.1) A. Testes: glands which produce male gametes, as well as glands producing testosterone 2. Seminiferous tubules (Fig.28.3; 28.5) a.
More informationREPRODUCCIÓN. La idea fija. Copyright 2004 Pearson Education, Inc., publishing as Benjamin Cummings
REPRODUCCIÓN La idea fija How male and female reproductive systems differentiate The reproductive organs and how they work How gametes are produced and fertilized Pregnancy, stages of development, birth
More informationAbstracts for the KSAR and JSAR Joint Symposium. Fertility control in female domestic animals: From basic understanding to application
Abstracts for the KSAR and JSAR Joint Symposium Fertility control in female domestic animals: From basic understanding to application Current Research Orientation in Livestock Reproduction in Korea Choong-Saeng
More informationStudy Guide Answer Key Reproductive System
Biology 12 Human Biology Textbook: BC Biology 12 Study Guide Answer Key Reproductive System 1. Distinguish between a gamete and a gonad using specific examples from the male and female systems. Gonads
More informationSmall Ruminant Embryo Transfer. - Opportunities and Challenges A Practitioner s perspective Brian W. McOnie
Small Ruminant Embryo Transfer - Opportunities and Challenges A Practitioner s perspective Brian W. McOnie Benefits of embryo transfer Embryo transfer (ET) technology gained commercial prominence in the
More informationCARD HyperOva (Superovulation Reagent for mouse)
Product manual (Superovulation Reagent for mouse) Cat. No. KYD-010-EX -X5 Size: 5 1 ML Origin Serum of goat, Horse-derived villus gonatropin. Composition 1. Inhibin antiserum (Goat). 2. Equine chorionic
More informationFemale Reproductive Physiology. Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF
Female Reproductive Physiology Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF REFERENCE Lew, R, Natural History of ovarian function including assessment of ovarian reserve
More informationPast, present and future of bovine in vitro embryo production in Denmark. Poul Hyttel and Lotte Strøbech University of Copenhagen
Past, present and future of bovine in vitro embryo production in Denmark Poul Hyttel and Lotte Strøbech University of Copenhagen Bovine in vitro embryo production in DK - looking back Royal Veterinary
More informationA comparison of the effects of estrus cow. nuclear maturation of bovine oocytes
A comparison of the effects of estrus cow serum and fetal calf serum on in vitro nuclear maturation of bovine oocytes J Spiropoulos, SE Long University of Bristol, School of Veterinary Science, Department
More informationPhases of the Ovarian Cycle
OVARIAN CYCLE An ovary contains many follicles, and each one contains an immature egg called an oocyte. A female is born with as many as 2 million follicles, but the number is reduced to 300,000 to 400,000
More informationFertility assessment and assisted conception
Fertility assessment and assisted conception Dr Geetha Venkat MD FRCOG Director Pulse Learning Women s health 14 September 2016 Disclosure statement Dr Venkat is a director of Harley Street Fertility Clinic.
More information9.4 Regulating the Reproductive System
9.4 Regulating the Reproductive System The Reproductive System to unite a single reproductive cell from a female with a single reproductive cell from a male Both male and female reproductive systems include
More informationWhy Cycle Control?" Manipulating Ovulation and Estrous Synchronization" Manipulating Ovulation" Cattle" Principle of PGF 2α Use"
Why Cycle Control?" Manipulating Ovulation and Estrous Synchronization" John Parrish 1. Group females for parturition: " a) Decrease labor, calving period Reduce calving season" b) More uniform weaning
More information