Chapter 23 *Lecture PowerPoint

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1 Chapter 23 *Lecture PowerPoint The Urinary System *See separate FlexArt PowerPoint slides for all figures and tables preinserted into PowerPoint without notes. Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

2 Introduction Urinary system rids the body of waste products. The urinary system is closely associated with the reproductive system Shared embryonic development and adult anatomical relationship Collectively called the urogenital (UG) system 23-2

3 Functions of the Urinary System Expected Learning Outcomes Name and locate the organs of the urinary system. List several functions of the kidneys in addition to urine formation. Name the major nitrogenous wastes and identify their sources. Define excretion and identify the systems that excrete wastes. 23-3

4 Functions of the Urinary System Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Diaphragm 11th and 12th ribs Adrenal gland Renal artery Renal vein Kidney Vertebra L2 Aorta Inferior vena cava Ureter Urinary bladder Urethra Figure 23.1a,b (a) Anterior view (b) Posterior view Urinary system consists of six organs: two kidneys, two ureters, urinary bladder, and urethra 23-4

5 Functions of the Kidneys Filters blood plasma, separates waste from useful chemicals, returns useful substances to blood, eliminates wastes Regulate blood volume and pressure by eliminating or conserving water Regulate the osmolarity of the body fluids by controlling the relative amounts of water and solutes eliminated Secretes enzyme, renin, which activates hormonal mechanisms that control blood pressure and electrolyte balance 23-5

6 Functions of the Kidneys Secretes the hormone, erythropoietin, which stimulates the production of red blood cells Collaborate with the lungs to regulate the PCO 2 and acid base balance of body fluids Final step in synthesizing hormone, calcitriol, which contributes to calcium homeostasis Gluconeogenesis from amino acids in extreme starvation 23-6

7 Retroperitoneal Position of the Kidney Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Anterior Small intestine Stomach Colon Pancreas Renal artery and vein Peritoneum Spleen Kidney Hilum Fibrous capsule Perirenal fat capsule Renal fascia Inferior vena cava Aorta L1 Ureter Lumbar muscles Posterior Figure 23.3a,b 23-7

8 Nitrogenous Wastes Waste any substance that is useless to the body or present in excess of the body s needs Metabolic waste waste substance produced by the body Urea formation Proteins amino acids NH 2 removed forms ammonia, liver converts to urea Uric acid Product of nucleic acid catabolism Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. H O N C H H H 2 N NH 2 Ammonia Urea O NH H C N C HN C C O HN N CH 3 O C C CH N N C 2 H H O Uric acid Creatinine Figure

9 Nitrogenous Wastes Creatinine Product of creatine phosphate catabolism Blood urea nitrogen (BUN) expression of the level of nitrogenous waste in the blood Normal concentration of blood urea is 10 to 20 mg/dl Azotemia: elevated BUN Indicates renal insufficiency Uremia: syndrome of diarrhea, vomiting, dyspnea, and cardiac arrhythmia stemming from the toxicity of nitrogenous waste Treatment hemodialysis or organ transplant Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. H O N C H H H 2 N NH 2 Ammonia Urea O NH H C N C HN C C O HN N CH 3 O C C CH N N C 2 H H O Uric acid Creatinine Figure

10 Excretion Excretion separation of wastes from body fluids and the elimination of them Four body systems carry out excretion Respiratory system CO 2, small amounts of other gases, and water Integumentary system Water, inorganic salts, lactic acid, urea in sweat Digestive system Water, salts, CO 2, lipids, bile pigments, cholesterol, other metabolic waste, and food residue Urinary system Many metabolic wastes, toxins, drugs, hormones, salts, H +, and water 23-10

11 Anatomy of the Kidney Expected Learning Outcomes Describe the location and general appearance of the kidney. Identify the external and internal features of the kidney. Trace the flow of blood through the kidney. Trace the flow of fluid through the renal tubules. Describe the nerve supply to the kidney

12 Position and Associated Structures Position, weight, and size Lie against posterior abdominal wall at level of T12 to L3 Right kidney is slightly lower due to large right lobe of liver Rib 12 crosses the middle of the left kidney Retroperitoneal along with ureters, urinary bladder, renal artery and vein, and adrenal glands 23-12

13 Shape and size Gross Anatomy About the size of a bar of bath soap Lateral surface is convex, and medial is concave with a slit, called the hilum Receives renal nerves, blood vessels, lymphatics, and ureter Three protective connective tissue coverings Renal fascia immediately deep to parietal peritoneum Binds it to abdominal wall Perirenal fat capsule: cushions kidney and holds it into place Fibrous capsule encloses kidney protecting it from trauma and infection Collagen fibers extend from fibrous capsule to renal fascia Still drop about 3 cm when going from lying down to standing up 23-13

14 Gross Anatomy of the Kidney Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fibrous capsule Renal cortex Renal medulla Renal papilla Renal sinus Adipose tissue in renal sinus Renal pelvis Major calyx Minor calyx Renal column Renal pyramid Ureter Renal blood vessels (a) Ralph Hutchings/Visuals Unlimited Figure 23.4a 23-14

15 Gross Anatomy Renal parenchyma glandular tissue that forms urine Appears C-shaped in frontal section Encircles the renal sinus Renal sinus: contains blood and lymphatic vessels, nerves, and urine-collecting structures Adipose fills the remaining cavity and holds structures into place 23-15

16 Gross Anatomy Two zones of renal parenchyma Outer renal cortex Inner renal medulla Renal columns extensions of the cortex that project inward toward sinus Renal pyramids 6 to 10 with broad base facing cortex and renal papilla facing sinus Lobe of the kidney: one pyramid and its overlying cortex Minor calyx: cup that nestles the papilla of each pyramid; collects it urine Major calyces: formed by convergence of two or three minor calyces Renal pelvis: formed by convergence of two or three major calyces Ureter: a tubular continuation of the pelvis that drains the urine down to the urinary bladder 23-16

17 Gross Anatomy of the Kidney Fibrous capsule Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Renal cortex Figure 23.4b Renal medulla Renal papilla Renal sinus Renal pelvis Major calyx Minor calyx Renal column Renal pyramid Ureter Renal blood vessels (b) 23-17

18 Renal Circulation Kidneys account for only 0.4% of body weight, they receive about 21% of the cardiac output (renal fraction) Renal artery divides into segmental arteries that give rise to: - Interlobar arteries: up renal columns, between pyramids - Arcuate arteries: over pyramids - Interlobular arteries: up into cortex - Branch into afferent arterioles: each supplying one nephron Leads to a ball of capillaries glomerulus 23-18

19 Renal Circulation Cont. - Blood is drained from the glomerulus by efferent arterioles - Lead to either peritubular capillaries or vasa recta around portion of the renal tubule - Interlobular veins or directly into arcuate veins interlobar veins Renal vein empties into inferior vena cava 23-19

20 Renal Circulation Figure 23.5a,b Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Interlobular artery and vein Interlobar artery and vein Aorta Inferior vena cava Renal medulla Segmental artery Renal a. Segmental a. Renal v. Renal cortex Renal artery and vein Interlobar a. Arcuate a. Interlobar v. Arcuate v. Interlobular a. Interlobular v. Arcuate artery and vein Afferent arteriole Glomerulus Peritubular capillaries Efferent arteriole Vasa recta (a) (b) Kidneys receive 21% of cardiac output 23-20

21 Renal Circulation In the cortex, peritubular capillaries branch off of the efferent arterioles supplying the tissue near the glomerulus, the proximal and distal convoluted tubules C o r t e x Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Cortical nephron Juxtamedullary nephron PCT DCT Peritubular capillaries Afferent arteriole Glomerulus Efferent arteriole Interlobular artery Interlobular vein In the medulla, the efferent arterioles give rise to the vasa recta, supplying the nephron loop portion of the Vasa recta Nephron loop Arcuate artery Arcuate vein Collecting duct nephron Figure 23.6 M e d u l l a Corticomedullary junction 23-21

22 The Nephron Each kidney has about 1.2 million nephrons Each composed of two principal parts Renal corpuscle: filters the blood plasma Renal tubule: long coiled tube that converts the filtrate into urine Renal corpuscle consists of the glomerulus and a two-layered glomerular (Bowman) capsule that encloses glomerulus Parietal (outer) layer of Bowman capsule is simple squamous epithelium Visceral (inner) layer of Bowman capsule consists of elaborate cells called podocytes that wrap around the capillaries of the glomerulus Capsular space separates the two layers of Bowman capsule 23-22

23 The Renal Corpuscle Key Afferent arteriole Flow of blood Flow of filtrate Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Glomerular capsule: Parietal layer Capsular space Podocytes of visceral layer Glomerulus Blood flow Efferent arteriole (a) Blood flow Proximal convoluted tubule Glomerular capillaries (podocytes and capillary wall removed) Figure 23.7a Glomerular filtrate collects in capsular space, flows into proximal convoluted tubule. Note the vascular and urinary poles. Note the afferent arteriole is larger than the efferent arteriole

24 The Nephron Vascular pole the side of the corpuscle where the afferent arterial enters the corpuscle and the efferent arteriole leaves Urinary pole the opposite side of the corpuscle where the renal tubule begins 23-24

25 The Renal Tubule Renal (uriniferous) tubule a duct that leads away from the glomerular capsule and ends at the tip of the medullary pyramid Divided into four regions Proximal convoluted tubule, nephron loop, distal convoluted tubule: parts of one nephron Collecting duct receives fluid from many nephrons Proximal convoluted tubule (PCT) arises from glomerular capsule Longest and most coiled region Simple cuboidal epithelium with prominent microvilli for majority of absorption 23-25

26 The Renal Tubule Nephron loop (loop of Henle) long U-shaped portion of renal tubule Descending limb and ascending limb Thick segments have simple cuboidal epithelium Initial part of descending limb and part or all of ascending limb Heavily engaged in the active transport of salts and have many mitochondria Thin segment has simple squamous epithelium Forms lower part of descending limb Cells very permeable to water Distal convoluted tubule (DCT) begins shortly after the ascending limb reenters the cortex Shorter and less coiled that PCT Cuboidal epithelium without microvilli DCT is the end of the nephron 23-26

27 The Renal Tubule Collecting duct receives fluid from the DCTs of several nephrons as it passes back into the medulla Numerous collecting ducts converge toward the tip of the medullary pyramid Papillary duct: formed by merger of several collecting ducts 30 papillary ducts end in the tip of each papilla Collecting and papillary ducts lined with simple cuboidal epithelium Flow of fluid from the point where the glomerular filtrate is formed to the point where urine leaves the body: glomerular capsule proximal convoluted tubule nephron loop distal convoluted tubule collecting duct papillary duct minor calyx major calyx renal pelvis ureter urinary bladder urethra 23-27

28 Microscopic Anatomy of the Nephron Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Renal capsule Renal cortex Nephron Collecting duct Efferent arteriole Renal medulla (a) Renal corpuscle: Glomerular capsule Glomerulus Minor calyx Renal papilla Cortical nephron Convoluted tubules (PCT and DCT) Afferent arteriole Proximal convoluted tubule (PCT) Distal convoluted tubule (DCT) Juxtamedullary nephron Cortex Medulla Nephron loop: Descending limb Ascending limb Collecting duct (CD) Thick segment Thin segment Key Flow of blood Nephron loops Flow of tubular fluid (b) Figure 23.8 Collecting duct Papillary duct (c)

29 Cortical and Juxtamedullary Nephrons C o r t e x M e d u l l a Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Juxtamedullary nephron PCT DCT Vasa recta Nephron loop Cortical nephron Peritubular capillaries Arcuate artery Arcuate vein Collecting duct Figure 23.6 Afferent arteriole Glomerulus Efferent arteriole Interlobular artery Interlobular vein Corticomedullary junction Cortical nephrons 85% of all nephrons Short nephron loops Efferent arterioles branch into peritubular capillaries around PCT and DCT Juxtamedullary nephrons 15% of all nephrons Very long nephron loops, maintain salinity gradient in the medulla and helps conserve water Efferent arterioles branch into vasa recta around long nephron loop 23-29

30 Renal Innervation Renal plexus nerves and ganglia wrapped around each renal artery Follows branches of the renal artery into the parenchyma of the kidney Issues nerve fibers to the blood vessels and convoluted tubules of the nephron 23-30

31 Renal Innervation Cont. Carries sympathetic innervation from the abdominal aortic plexus Stimulation reduces glomerular blood flow and rate of urine production Respond to falling blood pressure by stimulating the kidneys to secrete renin, an enzyme that activates hormonal mechanisms to restore blood pressure Carries parasympathetic innervation from the vagus nerve; increases rate of urine production 23-31

32 Urine Formation I: Glomerular Filtration Expected Learning Outcomes Describe the process by which the kidney filters the blood plasma, including the relevant cellular structure of the glomerulus. Explain the forces that promote and oppose filtration, and calculate the filtration pressure if given the magnitude of these forces. Describe how the nervous system, hormones, and the nephron itself regulate filtration

33 Basic Stages of Urine Formation Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Blood flow 1 Glomerular filtration Creates a plasma like filtrate of the blood Renal corpuscle 2 Tubular reabsorption Removes useful solutes from the filtrate, returns them to the blood Flow of filtrate Peritubular capillaries 3 Tubular secretion Removes additional wastes from the blood, adds them to the filtrate Renal tubule 4 Water conservation Removes water from the urine and returns it to blood; concentrates wastes H 2 O H 2 O H 2 O Figure 23.9 Urine 23-33

34 Urine Formation I: Glomerular Filtration Kidneys convert blood plasma to urine in three stages Glomerular filtration Tubular reabsorption and secretion Water conservation Glomerular filtrate the fluid in the capsular space Similar to blood plasma except that it has almost no protein Tubular fluid fluid from the proximal convoluted tubule through the distal convoluted tubule Substances have been removed or added by tubular cells Urine fluid that enters the collecting duct Undergoes little alteration beyond this point except for changes in water content 23-34

35 Structure of the Glomerulus Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Podocyte cell body Foot processes (separated by narrow filtration slits) (b) 5 mm Capsular space Podocyte Foot processes Filtration slits Basement membrane Filtration pore Endothelial cell Blood plasma Erythrocyte Figure 23.10b-c (c) 0.5 mm b: Don Fawcett/Photo Researchers, Inc.; c: Barry F. King/Biological Photo Service

36 The Glomerular Filtration Membrane Turned back: Blood cells Plasma proteins Large anions Protein-bound minerals and hormones Most molecules > 8 nm in diameter Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Endothelial cell of glomerular capillary Basement membrane Filtration slit Filtration pore Foot process of podocyte Passed through filter: Water Electrolytes Glucose Amino acids Fatty acids Vitamins Urea Uric acid Creatinine Figure Bloodstream Capsular space 23-36

37 The Filtration Membrane Glomerular filtration a special case of the capillary fluid exchange process in which water and some solutes in the blood plasma pass from the capillaries of the glomerulus into the capsular space of the nephron 23-37

38 The Filtration Membrane Filtration membrane three barriers through which fluid passes Fenestrated endothelium of glomerular capillaries 70 to 90 nm filtration pores exclude blood cells Highly permeable Basement membrane Proteoglycan gel, negative charge, excludes molecules greater than 8 nm Albumin repelled by negative charge Blood plasma is 7% protein, the filtrate is only 0.03% protein Filtration slits Podocyte cell extensions (pedicels) wrap around the capillaries to form a barrier layer with 30 nm filtration slits Negatively charged which is an additional obstacle for large anions 23-38

39 The Filtration Membrane Almost any molecule smaller than 3 nm can pass freely through the filtration membrane Water, electrolytes, glucose, fatty acids, amino acids, nitrogenous wastes, and vitamins Some substances of low molecular weight are bound to the plasma proteins and cannot get through the membrane Most calcium, iron, and thyroid hormone Unbound fraction passes freely into the filtrate 23-39

40 The Filtration Membrane Kidney infections and trauma can damage the filtration membrane and allow albumin or blood cells to filter Proteinuria (albuminuria): presence of protein in urine Hematuria: presence of blood in the urine Distance runners and swimmers often experience temporary proteinuria or hematuria Prolonged, strenuous exercise greatly reduces profusion of kidney Glomerulus deteriorates under prolonged hypoxia 23-40

41 Filtration Pressure Blood hydrostatic pressure (BHP) Much higher in glomerular capillaries (60 mm Hg compared to 10 to 15 in most other capillaries) Because afferent arteriole is larger than efferent arteriole Larger inlet and smaller outlet Hydrostatic pressure in capsular space 18 mm Hg due to high filtration rate and continual accumulation of fluid in the capsule 23-41

42 Filtration Pressure Colloid osmotic pressure (COP) of blood About the same here as elsewhere: 32 mm Hg Glomerular filtrate is almost protein-free and has no significant COP Higher outward pressure of 60 mm Hg, opposed by two inward pressures of 18 mm Hg and 32 mm Hg Net filtration pressure: 60 out 18 in 32 in = 10 mm Hg out 23-42

43 The Forces Involved in Glomerular Filtration Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. BHP 60 out COP 32 in CP 18 in Blood hydrostatic pressure (BHP) Colloid osmotic pressure (COP) Capsular pressure (CP) Net filtration pressure (NFP) Figure NFP 10 out 60 mm Hg out -32 mm Hg in -18 mm Hg in 10 mm Hg out High BP in glomerulus makes kidneys vulnerable to hypertension It can lead to rupture of glomerular capillaries, produce scarring of the kidneys (nephrosclerosis), and atherosclerosis of renal blood vessels, ultimately leading to renal failure 23-43

44 Glomerular Filtration Rate Glomerular filtration rate (GFR) the amount of filtrate formed per minute by the two kidneys combined GFR = NFP x K f 125 ml/min. or 180 L/day (male) GFR = NFP x K f 105 ml/min. or 150 L/day (female) Net filtration pressure (NFP) Filtration coefficient (K f ) depends on permeability and surface area of filtration barrier 23-44

45 Glomerular Filtration Rate Total amount of filtrate produced equals 50 to 60 times the amount of blood in the body 99% of filtrate is reabsorbed since only 1 to 2 L urine excreted per day 23-45

46 Regulation of Glomerular Filtration GFR too high Fluid flows through the renal tubules too rapidly for them to reabsorb the usual amount of water and solutes Urine output rises Chance of dehydration and electrolyte depletion GFR too low Wastes reabsorbed Azotemia may occur 23-46

47 Regulation of Glomerular Filtration GFR controlled by adjusting glomerular blood pressure from moment to moment GFR control is achieved by three homeostatic mechanisms Renal autoregulation Sympathetic control Hormonal control 23-47

48 Renal Autoregulation Renal autoregulation the ability of the nephrons to adjust their own blood flow and GFR without external (nervous or hormonal) control Enables them to maintain a relatively stable GFR in spite of changes in systemic arterial blood pressure Two methods of autoregulation: myogenic mechanism and tubuloglomerular feedback 23-48

49 Renal Autoregulation Myogenic mechanism based on the tendency of smooth muscle to contract when stretched Increased arterial blood pressure stretches the afferent arteriole Arteriole constricts and prevents blood flow into the glomerulus from changing much When blood pressure falls The afferent arteriole relaxes Allows blood flow more easily into glomerulus Filtration remains stable 23-49

50 Renal Autoregulation Tubuloglomerular feedback mechanism by which glomerulus receives feedback on the status of the downstream tubular fluid and adjusts filtration to regulate the composition of the fluid, stabilize its own performance, and compensate for fluctuation in systemic blood pressure Juxtaglomerular apparatus: complex structure found at the very end of the nephron loop where it has just reentered the renal cortex Loop comes into contact with the afferent and efferent arterioles at the vascular pole of the renal corpuscle 23-50

51 Renal Autoregulation Three special kinds of cells occur in the juxtaglomerular apparatus Macula densa patch of slender, closely spaced epithelial cells at end of the nephron loop on the side of the tubules facing the arterioles Senses variations in flow or fluid composition and secretes a paracrine that stimulates JG cells 23-51

52 Cont. Renal Autoregulation Juxtaglomerular (JG) cells: enlarged smooth muscle cells in afferent arteriole directly across from macula densa When stimulated by the macula They dilate or constrict the arterioles They also contain granules of renin, which they secrete in response to drop in blood pressure Mesangial cells: in cleft between afferent and efferent arterioles and among capillaries of the glomerulus Connected to macula densa and JG cells by gap junctions and communicate by means of paracrines Build supportive matrix for glomerulus, constrict or relax capillaries to regulate flow 23-52

53 The Juxtaglomerular Apparatus Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Podocytes Mesangial cells Efferent arteriole Nephron loop Figure Sympathetic nerve fiber Juxtaglomerular cells Afferent arteriole Smooth muscle cells Macula densa If GFR rises Flow of tubular fluid increases and more NaCl is reabsorbed Macula densa stimulates JG cells with a paracrine JG cells contract which constricts afferent arteriole, reducing GFR to normal OR Mesangial cells may contract, constricting the capillaries and reducing filtration If GFR falls Macula relaxes afferent arterioles and mesangial cells Blood flow increases and GFR rises back to normal 23-53

54 Renal Autoregulation Maintains a dynamic equilibrium GFR fluctuates within narrow limits only Blood pressure changes do affect GFR and urine output somewhat Renal autoregulation cannot compensate for extreme blood pressure variation Over a MAP range of 90 to 180 mm Hg, the GFR remains quite stable Below 70 mm Hg, glomerular filtration and urine output cease Occurs in hypovolemic shock 23-54

55 Negative Feedback Control of GFR Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. High GFR Reduced GFR Rapid flow of filtrate in renal tubules Constriction of afferent arteriole Sensed by macula densa Paracrine secretion Figure

56 Sympathetic Control Sympathetic nerve fibers richly innervate the renal blood vessels Sympathetic nervous system and adrenal epinephrine constrict the afferent arterioles in strenuous exercise or acute conditions like circulatory shock Reduces GFR and urine output Redirects blood from the kidneys to the heart, brain, and skeletal muscles GFR may be as low as a few milliliters per minute 23-56

57 Renin Angiotensin Aldosterone Mechanism Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Drop in blood pressure Kidney Hypothalamus Liver Angiotensinogen (453 amino acids long) Renin Angiotensin I (10 amino acids long) Angiotensin II (8 amino acids long) Vasoconstriction Angiotensinconverting enzyme (ACE) Cardiovascular system Aldosterone Lungs Adrenal cortex Kidney Renin secreted by juxtaglomerular cells if BP drops dramatically Renin converts angiotensinogen, a blood protein, into angiotensin I In the lungs and kidneys, angiotensin-converting enzyme (ACE) converts angiotensin I to angiotensin II, the active hormone Works in several ways to restore fluid volume and BP Thirst and drinking Elevated blood pressure Sodium and water retention Figure

58 The Renin Angiotensin Aldosterone Mechanism Angiotensin II Potent vasoconstrictor raising BP throughout body Constricts efferent arteriole raising GFR despite low BP Lowers BP in peritubular capillaries enhancing reabsorption of NaCl and H 2 O Angiotensin II stimulates adrenal cortex to secrete aldosterone promoting Na + and H 2 O reabsorption in DCT and collecting duct Stimulates posterior pituitary to secrete ADH which promotes water reabsorption by collecting duct Stimulates thirst and H 2 O intake 23-58

59 Urine Formation II: Tubular Reabsorption and Secretion Expected Learning Outcomes Describe how the renal tubules reabsorb useful solutes from the glomerular filtrate and return them to the blood. Describe how the tubules secrete solute from the blood into the tubular fluid. Describe how the nephron regulates water excretion

60 Basic Stages of Urine Formation Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Blood flow 1 2 Glomerular filtration Creates a plasma like filtrate of the blood Tubular reabsorption Removes useful solutes from the filtrate, returns them to the blood Renal corpuscle Flow of filtrate Peritubular capillaries Conversion of glomerular filtrate to urine involves the removal and addition of chemicals by tubular reabsorption and secretion Occurs through PCT to DCT Tubular fluid is modified 3 Tubular secretion Removes additional wastes from the blood, adds them to the filtrate Steps involved include: Renal tubule Tubular reabsorption 4 Water conservation Removes water from the urine and returns it to blood; concentrates wastes H 2 O H 2 O H 2 O Tubular secretion Water conservation Urine Figure

61 The Proximal Convoluted Tubule PCT reabsorbs about 65% of glomerular filtrate, removes some substances from the blood, and secretes them into the tubular fluid for disposal in urine Prominent microvilli and great length Abundant mitochondria provide ATP for active transport PCTs alone account for about 6% of one s resting ATP and calorie consumption Tubular reabsorption process of reclaiming water and solutes from the tubular fluid and returning them to the blood 23-61

62 The Proximal Convoluted Tubule Two routes of reabsorption Transcellular route Substances pass through the cytoplasm of the PCT epithelial cells and out their base Paracellular route Substances pass between PCT cells Junctions between epithelial cells are quite leaky and allow significant amounts of water to pass through Solvent drag water carries with it a variety of dissolved solutes Taken up by peritubular capillaries 23-62

63 Tubular Reabsorption Sodium reabsorption is the key to everything else Creates an osmotic and electrical gradient that drives the reabsorption of water and other solutes Most abundant cation in filtrate Creates steep concentration gradient that favors its diffusion into the epithelial cells Two types of transport proteins in the apical cell surface are responsible for sodium uptake Symports that simultaneously bind Na + and another solute such as glucose, amino acids, or lactate Na + H + antiport that pulls Na + into the cell while pumping out H + into tubular fluid 23-63

64 Tubular Reabsorption Sodium is prevented from accumulating in the epithelial cells by Na + K + pumps in the basal surface of the epithelium Pumps Na + out into the extracellular fluid Picked up by peritubular capillaries and returned to the bloodstream ATP consuming active transport pumps Secondary active transport: Na + transporting symports in apical cell membrane do not consume ATP, are considered an example of secondary active transport for their dependence on the Na + K + pumps at the base of the cell 23-64

65 Tubular Reabsorption Negative chloride ions follow the positive sodium ions by electrical attraction Various antiports in the apical cell membrane that absorb Cl in exchange for other anions they eject into the tubular fluid: K + Cl symport 23-65

66 Tubular Reabsorption Potassium, magnesium, and phosphate ions diffuse through the paracellular route with water Phosphate is also cotransported into the epithelial cells with Na + Some calcium is reabsorbed through the paracellular route in the PCT, but most Ca 2+ occurs later in the nephron Peritubular capillary Tissue fluid Tubule epithelial cells Tubular fluid Glucose Na + K + K + Cl ATP K + Cl symport Na + K + pump ADP + P i Tight junction Na+ Glucose Na + Anions H + Cl H 2 O Sodium glucose transport protein (SGLT) (symport) Na + H + antiport Cl anion antiport Aquaporin Figure Solvent drag Transcellular route Paracellular route Brush border H 2 O, urea, uric acid, Na +, K +, Cl, Mg 2+, Ca 2+, P i Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display

67 Tubular Reabsorption Glucose is cotransported with Na + by sodium glucose transport (SGLT) proteins Urea diffuses through the tubule epithelium with water reabsorbs 40% to 60% in tubular fluid Kidneys remove about half of the urea from the blood; creatinine is not reabsorbed at all Peritubular capillary Tissue fluid Tubule epithelial cells Tubular fluid Glucose Na + K + K + Cl ATP K + Cl symport Na + K + pump ADP + P i Tight junction Na+ Glucose Na + Anions H + Cl H 2 O Sodium glucose transport protein (SGLT) (symport) Na + H + antiport Cl anion antiport Aquaporin Figure Solvent drag Transcellular route Paracellular route Brush border H 2 O, urea, uric acid, Na +, K +, Cl, Mg 2+, Ca 2+, P i Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display

68 Tubular Reabsorption Kidneys reduce 180 L of glomerular filtrate to 1 or 2 L urine each day Two-thirds of water in filtrate is reabsorbed by the PCT Reabsorption of all the salt and organic solutes makes the tubule cells and tissue fluid hypertonic Water follows solutes by osmosis through both paracellular and transcellular routes through water channels called aquaporins In PCT, water is reabsorbed at constant rate called obligatory water reabsorption 23-68

69 Uptake by the Peritubular Capillaries After water and solutes leave the basal surface of the tubular epithelium, they are reabsorbed by the peritubular capillaries Reabsorbed by osmosis and solvent drag Three factors promote osmosis into the capillaries Accumulation of reabsorbed fluid around the basolateral sides of epithelial cell creates high interstitial fluid pressure that drives water into the capillaries 23-69

70 Uptake by the Peritubular Capillaries Cont. Narrowness of efferent arterioles lowers blood hydrostatic pressure in peritubular capillaries so there is less resistance to absorption Proteins remain in blood after filtration, which elevates colloid osmotic pressure High COP and low BHP in the capillaries and high hydrostatic pressure in the tissue fluid, the balance of forces in the peritubular capillaries favors absorption 23-70

71 The Transport Maximum Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. (a) Normoglycemia Normal urine volume, glucose-free Glomerular filtration Glucose transport protein Glucose reabsorption (b) Figure Hyperglycemia Increased urine volume, with glycosuria There is a limit to the amount of solute that the renal tubules can reabsorb Limited by the number of transport proteins in the plasma membrane If all transporters are occupied as solute molecules pass Excess solutes appear in urine Transport maximum is reached when transporters are saturated Each solute has its own transport maximum Any blood glucose level above 220 mg/dl results in glycosuria 23-71

72 Tubular Secretion Tubular secretion process in which renal tubule extracts chemicals from capillary blood and secretes them into tubular fluid Two purposes in proximal convoluted tubule and nephron loop Waste removal Urea, uric acid, bile acids, ammonia, catecholamines, prostaglandins, and a little creatinine are secreted into the tubule Secretion of uric acid compensates for its reabsorption earlier in PCT Clears blood of pollutants, morphine, penicillin, aspirin, and other drugs Explains need to take prescriptions three to four times/day to keep pace with the rate of clearance Acid base balance Secretion of hydrogen and bicarbonate ions help regulate ph of body fluids 23-72

73 The Nephron Loop Primary function of nephron loop is to generate salinity gradient that enables collecting duct to concentrate the urine and conserve water Electrolyte reabsorption from filtrate Thick segment reabsorbs 25% of Na +, K +, and Cl Ions leave cells by active transport and diffusion NaCl remains in the tissue fluid of renal medulla Water cannot follow since thick segment is impermeable Tubular fluid very dilute as it enters distal convoluted tubule 23-73

74 The Distal Convoluted Tubule and Collecting Duct Fluid arriving in the DCT still contains about 20% of the water and 7% of the salts from glomerular filtrate If this were all passed as urine, it would amount to 36 L/day DCT and collecting duct reabsorb variable amounts of water salt and are regulated by several hormones Aldosterone, atrial natriuretic peptide, ADH, and parathyroid hormone 23-74

75 The Distal Convoluted Tubule and Collecting Duct Two kinds of cells in the DCT and collecting duct Principal cells Most numerous Have receptors for hormones Involved in salt and water balance Intercalated cells Involved in acid base balance by secreting H + into tubule lumen and reabsorbing K

76 The Distal Convoluted Tubule and Collecting Duct Aldosterone the salt-retaining hormone Steroid secreted by the adrenal cortex When blood Na + concentration falls or When K + concentration rises or There is a drop in blood pressure renin release angiotensin II formation stimulates adrenal cortex to secrete aldosterone 23-76

77 The Distal Convoluted Tubule and Collecting Duct Functions of aldosterone Acts on thick segment of nephron loop, DCT, and cortical portion of collecting duct Stimulates the reabsorption of more Na + and secretion of K + Water and Cl follow the Na + Net effect is that the body retains NaCl and water Helps maintain blood volume and pressure Urine volume is reduced Urine has an elevated K + concentration 23-77

78 The Distal Convoluted Tubule and Collecting Duct Atrial natriuretic peptide (ANP) secreted by atrial myocardium of the heart in response to high blood pressure Four actions result in the excretion of more salt and water in the urine, thus reducing blood volume and pressure Dilates afferent arteriole, constricts efferent arteriole: GFR Inhibits renin and aldosterone secretion Inhibits secretion of ADH Inhibits NaCl reabsorption by collecting duct 23-78

79 The Distal Convoluted Tubule and Collecting Duct Antidiuretic hormone (ADH) secreted by posterior lobe of pituitary In response to dehydration and rising blood osmolarity Stimulates hypothalamus Hypothalamus stimulates posterior pituitary Action make collecting duct more permeable to water Water in the tubular fluid reenters the tissue fluid and bloodstream rather than being lost in urine 23-79

80 The Distal Convoluted Tubule and Collecting Duct Parathyroid hormone (PTH) secreted from parathyroid glands in response to calcium deficiency (hypocalcemia) Acts on PCT to increase phosphate excretion Acts on the thick segment of the ascending limb of the nephron loop, and on the DCT to increase calcium reabsorption Increases phosphate content and lowers calcium content in urine Because phosphate is not retained, the calcium ions stay in circulation rather than precipitating into the bone tissue as calcium phosphate PTH stimulates calcitriol synthesis by epithelial cells of the PCT 23-80

81 The Distal Convoluted Tubule and Collecting Duct PCT reabsorbs 65% of glomerular filtrate and returns it to peritubular capillaries Much reabsorption by osmosis and cotransport mechanisms linked to active transport of sodium Nephron loop reabsorbs another 25% of filtrate DCT reabsorbs Na +, Cl, and water under hormonal control, especially aldosterone and ANP The tubules also extract drugs, wastes, and some solutes from the blood and secrete them into the tubular fluid DCT completes the process of determining the chemical composition of urine Collecting duct conserves water 23-81

82 Urine Formation III: Water Conservation Expected Learning Outcomes Explain how the collecting duct and antidiuretic hormone regulate the volume and concentration of urine. Explain how the kidney maintains an osmotic gradient in the renal medulla that enables the collecting duct to function

83 Urine Formation III: Water Conservation The kidney eliminates metabolic wastes from the body, but prevents excessive water loss as well As the kidney returns water to the tissue fluid and bloodstream, the fluid remaining in the renal tubules passes as urine, and becomes more concentrated 23-83

84 Osmolarity of tissue fluid (mosm/l) Water Reabsorption by the Collecting Duct Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Tubular fluid (300 mosm/l) ,200 H 2 O H 2 O H 2 O H 2 O H 2 O Cortex Medulla Collecting duct Nephron loop Collecting duct (CD) begins in the cortex where it receives tubular fluid from several nephrons As CD passes through the medulla, it reabsorbs water and concentrates urine up to four times Medullary portion of CD is more permeable to water than to NaCl As urine passes through the increasingly salty medulla, water leaves by osmosis, concentrating urine Urine (up to 1,200 mosm/l) Figure

85 Control of Water Loss How concentrated the urine becomes depends on body s state of hydration Water diuresis drinking large volumes of water will produce a large volume of hypotonic urine Cortical portion of CD reabsorbs NaCl, but it is impermeable to water Salt removed from the urine stays in the CD Urine concentration may be as low as 50 mosm/l 23-85

86 Control of Water Loss Producing hypertonic urine Dehydration causes the urine to become scanty and more concentrated High blood osmolarity stimulates posterior pituitary to release ADH and then an increase in synthesis of aquaporin channels by renal tubule cells More water is reabsorbed by collecting duct Urine is more concentrated If BP is low in a dehydrated person, GFR will be low Filtrate moves more slowly and more time for reabsorption More salt removed, more water reabsorbed, and less urine produced 23-86

87 The Countercurrent Multiplier The ability of kidney to concentrate urine depends on salinity gradient in renal medulla Four times as salty in the renal medulla than the cortex Nephron loop acts as countercurrent multiplier Multiplier: continually recaptures salt and returns it to extracellular fluid of medulla which multiplies the salinity in adrenal medulla Countercurrent : because of fluid flowing in opposite directions in adjacent tubules of nephron loop Fluid flowing downward in descending limb Passes through environment of increasing osmolarity Most of descending limb very permeable to water but not to NaCl Water passes from tubule into the ECF leaving salt behind Concentrates tubular fluid to 1,200 mosm/l at lower end of loop 23-87

88 The Countercurrent Multiplier Fluid flowing upward in ascending limb Impermeable to water Reabsorbs Na +, K +, and Cl by active transport pumps into ECF Maintains high osmolarity of renal medulla Tubular fluid becomes hypotonic: 100 mosm/l at top of loop Recycling of urea: lower end of CD permeable to urea Urea contributes to the osmolarity of deep medullary tissue Continually cycled from collecting duct to the nephron loop and back Urea remains concentrated in the collecting duct and some of it always diffuses out into the medulla adding to osmolarity 23-88

89 Countercurrent Multiplier of Nephron Loop 1 More salt is continually added by the PCT. Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display The more salt that is pumped out of the ascending limb, the saltier the ECF is in the renal medulla. 2 The higher the osmolarity of the ECF, the more water leaves the descending limb by osmosis. 400 Na + K + Cl 200 Na + K + Cl H 2 O 600 H 2 O Na + K + Cl 400 Na + K + Cl H 2 O 3 The more water that leaves the descending limb, the saltier the fluid is that remains in the tubule. H 2 O 900 Na + K + Cl H 2 O Na + K + Cl The saltier the fluid in the ascending limb, the more salt the tubule pumps into the ECF. 1,200 Figure

90 The Countercurrent Exchange System Vasa recta capillary branching off efferent arteriole in medulla Provides blood supply to medulla and does not remove NaCl and urea from medullary ECF Countercurrent system formed by blood flowing in opposite directions in adjacent parallel capillaries Descending capillaries Exchanges water for salt Water diffuses out of capillaries and salt diffuses in 23-90

91 The Countercurrent Exchange System As blood flows back up to the cortex, the opposite occurs Ascending capillaries Exchanges salt for water Water diffuses into and NaCl diffuses out of blood Vasa recta gives the salt back and does not subtract from the osmolarity of the medulla Absorb more water on way out than the way in, and thus they carry away water reabsorbed from the urine by collecting duct and nephron loop 23-91

92 Functional Relationship of the Nephron Loop Figure Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Osmolarity of ECF (mosm/l) Cortex Medulla Urea Na + K + Cl Na + K + Cl H 2 O Na + K + Cl Na + K + Cl Urea 500 Urea H 2 O NaCl H 2 O NaCl 900 Key Active transport H 2 O Urea 900 Na + K + Cl Urea Urea 700 Urea H 2 O NaCl H 2 O NaCl 1,200 Diffusion through a membrane channel H 2 O 1,200 1,200 1,200 Nephron loop Collecting duct Vasa recta 23-92

93 Solutes Reabsorbed and Secreted Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Glucose Na + Amino acids K + Protein Ca 2+ Vitamins Mg 2+ Lactate Cl Urea HCO 3 Uric acid H 2 O PCT Na + Cl HCO 3 H 2 O DCT Urea H + Uric acid NH + 4 Creatinine Some drugs Nephron loop: Descending limb Ascending limb Na + K + Cl H + K + NH 4 + Collecting duct H 2 O Urea H 2 O Urea Key Tubular reabsorption Tubular secretion Figure

94 Urine and Renal Function Tests Expected Learning Outcomes Describe the composition and properties of urine. Carry out some calculations to evaluate renal function

95 Composition and Properties of Urine Urinalysis the examination of the physical and chemical properties of urine Appearance clear, almost colorless to deep amberyellow color due to urochrome pigment from breakdown of hemoglobin (RBCs); other colors from foods, drugs, or diseases Cloudiness or blood could suggest urinary tract infection, trauma, or stones Pyuria: pus in the urine Hematuria: blood in urine due to urinary tract infection, trauma, or kidney stones Odor bacteria degrade urea to ammonia, some foods impart aroma 23-95

96 Composition and Properties of Urine Specific gravity compared to distilled water Density of urine ranges from to1.028 g/ml Osmolarity (blood = 300 mosm/l) Ranges from 50 mosm/l to 1,200 mosm/l in dehydrated person ph range: 4.5 to 8.2, usually 6.0 (mildly acidic) Chemical composition: 95% water, 5% solutes Normal to find: urea, NaCl, KCl, creatinine, uric acid, phosphates, sulfates, traces of calcium, magnesium, and sometimes bicarbonate, urochrome, and a trace of bilirubin Abnormal to find: glucose, free hemoglobin, albumin, ketones, bile pigments 23-96

97 Urine Volume Normal volume for average adult 1 to 2 L/day Polyuria output in excess of 2 L/day Oliguria output of less than 500 ml/day Anuria 0 to 100 ml/day Low output from kidney disease, dehydration, circulatory shock, prostate enlargement Low urine output of less than 400 ml/day, the body cannot maintain a safe, low concentration of waste in the plasma 23-97

98 Urine Volume Diabetes any metabolic disorder resulting in chronic polyuria At least four forms of diabetes Diabetes mellitus type 1, type 2, and gestational diabetes High concentration of glucose in renal tubule Glucose opposes the osmotic reabsorption of water More water passes in urine (osmotic diuresis) Glycosuria glucose in the urine Diabetes insipidus ADH hyposecretion causes not enough water to be reabsorbed in the collecting duct More water passes in urine 23-98

99 Urine Volume Diuretics any chemical that increases urine volume Some increase GFR Caffeine dilates the afferent arteriole Reduce tubular reabsorption of water Alcohol inhibits ADH secretion Act on nephron loop (loop diuretic): inhibit Na + K + Cl symport Impairs countercurrent multiplier reducing the osmotic gradient in the renal medulla Collecting duct unable to reabsorb as much water as usual Commonly used to treat hypertension and congestive heart failure by reducing the body s fluid volume and blood pressure 23-99

100 Renal Function Tests Tests for diagnosing kidney disease Evaluating their severity Monitoring their progress Determining renal clearance Determining glomerular filtration rate

101 Renal Clearance Renal clearance the volume of blood plasma from which a particular waste is completely removed in 1 minute Represents the net effect of three processes Glomerular filtration of the waste + Amount added by tubular secretion Amount removed by tubular reabsorption Renal clearance

102 Renal Clearance Determine renal clearance (C) by collecting blood and urine samples, measuring the waste concentration in each, and measuring the rate of urine output U: waste concentration in urine: 6.0 mg/ml (urea example) V: rate of urine output: 2 ml/min. P: waste concentration in plasma: 0.2 mg/ml C: renal clearance in ml/min. of waste cleared C = UV/P = 60 ml/min (60 ml of blood plasma is completely cleared of urea per minute) Compare C to normal GFR of 125 ml/min. to see if normal rate of clearance is occurring 48%, which is normal for urea

103 Glomerular Filtration Rate Kidney disease often results in lowering of GFR need to measure patient s GFR Cannot use clearance rate of urea Some urea filtered by glomerulus is reabsorbed in the tubule Some urea is secreted into the tubule Need a substance that is not secreted or reabsorbed at all so that all of it in the urine gets there by glomerular filtration Use inulin, a plant polysaccharide to determine GFR Neither reabsorbed or secreted by the renal tubule Inulin GFR = renal clearance on inulin Clinically GFR is estimated from creatinine excretion Does not require injecting a substance or drawing blood to determine its blood concentration

104 Urine Storage and Elimination Expected Learning Outcomes Describe the functional anatomy of the ureters, urinary bladder, and male and female urethra. Explain how the nervous system and urethral sphincters control the voiding of urine

105 Urine Storage and Elimination Urine is produced continually Does not drain continually from the body Urination is episodic occurring when we allow it Made possible by storage apparatus and neural controls of this timely release

106 The Ureters Ureters retroperitoneal, muscular tube that extends from the kidney to the urinary bladder About 25 cm long Passes posterior to bladder and enters it from below Flap of mucosa acts as a valve into bladder Keeps urine from backing up in the ureter when bladder contracts

107 The Ureters Cont. Three layers of ureter Adventitia connective tissue layer that connects ureter to surrounding structures Muscularis two layers of smooth muscle with third layer in lower ureter Urine enters, it stretches and contracts in peristaltic wave Mucosa transitional epithelium Begins at minor calyces and extends through the bladder Lumen very narrow, easily obstructed kidney stones

108 The Urinary Bladder Urinary bladder muscular sac located on floor of pelvic cavity Inferior to peritoneum and posterior to pubic symphysis Three layers Parietal peritoneum, superiorly, fibrous adventitia other areas Muscularis: detrusor muscle: three layers of smooth muscle Mucosa: transitional epithelium Rugae conspicuous wrinkles in relaxed bladder

109 The Urinary Bladder Trigone smooth-surfaced triangular area marked with openings of ureters and urethra Capacity moderate fullness is 500 ml, maximum fullness is 700 to 800 ml Highly distensible As it fills, it expands superiorly Rugae flatten Epithelium thins from five or six layers to two or three

110 The Urinary Bladder Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Ureter Detrusor muscle Ureteral openings Figure 23.23a Trigone Urethra Internal urethral sphincter Urogenital diaphragm External urethral orifice (a) Female External urethral sphincter

111 Kidney Stones Renal calculus (kidney stone) hard granule of calcium phosphate, calcium oxalate, uric acid, or a magnesium salt called struvite Form in the renal pelvis Usually small enough to pass unnoticed in the urine flow Large stones might block renal pelvis or ureter and can cause pressure buildup in kidney which destroys nephrons Passage of large jagged stones is excruciatingly painful and may damage ureter causing hematuria

112 Kidney Stones Causes include hypercalcemia, dehydration, ph imbalances, frequent urinary tract infections, or enlarged prostate gland causing urine retention Treatment includes stone-dissolving drugs, often surgery, or lithotripsy (nonsurgical technique that pulverizes stones with ultrasound)

113 The Urethra Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Ureter Detrusor muscle Ureteral openings Trigone Urethra Urogenital diaphragm External urethral orifice (a) Female Figure 23.23a Internal urethral sphincter External urethral sphincter Female urethra: 3 to 4 cm long Bound to anterior wall of vagina External urethral orifice Between vaginal orifice and clitoris Internal urethral sphincter Detrusor muscle thickening Smooth muscle under involuntary control External urethral sphincter Where the urethra passes through the pelvic floor Skeletal muscle under voluntary control

114 The Urethra Ureter Rugae Ureteral openings Trigone Prostate gland Prostatic urethra Membranous urethra Bulbourethral gland Spongy (penile) urethra Penis Detrusor muscle Internal urethral sphincter Urogenital diaphragm External urethral sphincter External urethral orifice (b) Male Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Male urethra: 18 cm long Three regions Prostatic urethra (2.5 cm) Passes through prostate gland Membranous urethra (0.5 cm) Passes through muscular floor of pelvic cavity Spongy (penile) urethra (15 cm) Passes through penis in corpus spongiosum Internal urethral sphincter Detrusor muscle thickening External urethral sphincter Part of skeletal muscle of pelvic Figure 23.23b floor

115 Urinary Tract Infection (UTI) Cystitis infection of the urinary bladder Especially common in females due to short urethra Frequently triggered by sexual intercourse Can spread up the ureter causing pyelitis Pyelitis infection of the renal pelvis Pyelonephritis infection that reaches the cortex and the nephrons Can result from blood-borne bacteria

116 Voiding Urine Between acts of urination, the bladder is filling Detrusor muscle relaxes Urethral sphincters are tightly closed Accomplished by sympathetic pathway from upper lumbar spinal cord Postganglionic fibers travel through the hypogastric nerve to the detrusor muscle (relax) and internal urethral sphincter (excite)

117 Voiding Urine Cont. Somatic motor fibers from upper sacral spinal cord through pudendal nerve to supply the external sphincter give us voluntary control Micturition the act of urinating Micturition reflex spinal reflex that partly controls urination

118 Voiding Urine Involuntary control (steps 1 4) Stretch receptors detect filing of bladder, transmit afferent signals to the spinal cord Signals return to bladder from spinal cord (S2 or S3) via parasympathetic fibers in the pelvic nerve Efferent signals excite detrusor muscle Efferent signals relax internal urethral sphincter. Urine is involuntary voided if not inhibited by the brain

119 Voiding Urine Voluntary control (steps 5 8) For voluntary control, the micturition center in the pons receives signals from stretch receptors If it is timely to urinate, the pons returns signals to spinal interneurons that excite detrusor and relax internal urethral sphincter. Urine is voided. If it is untimely to urinate, signals from the pons excite spinal interneurons that keep external urethral sphincter contracted. Urine is retained in the bladder If it is timely to urinate, signals from the pons cease, and external urethral sphincter relaxes. Urine is voided

120 Neural Control of Micturition Full urinary bladder Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Involuntary micturition reflex Sensory fiber Pelvic nerve 1 Motor fiber To pons From pons Sacral segments of spinal cord S2 S Stretch receptors detect filling of bladder, transmit afferent signals to spinal cord. Signals return to bladder from spinal cord segments S2 and S3 via parasympathetic fibers in pelvic nerve. Efferent signals excite detrusor muscle. Efferent signals relax internal urethral sphincter. Urine is involuntarily voided if not inhibited by brain. Voluntary control For voluntary control, micturition center in pons receives signals from stretch receptors. Stretch receptors Motor fibers to detrusor muscle Internal urethral sphincter (involuntary) External urethral sphincter (voluntary) 4 Urethra 8 3 Parasympathetic ganglion in bladder wall Somatic motor fiber of pudendal nerve Figure S If it is timely to urinate, pons returns signals to spinal interneurons that excite detrusor and relax internal urethral sphincter. Urine is voided. If it is untimely to urinate, signals from pons excite spinal interneurons that keep external urethral sphincter contracted. Urine is retained in bladder. If it is timely to urinate, signals from pons cease and external urethral sphincter relaxes. Urine is voided

121 Voiding Urine There are times when the bladder is not full enough to trigger the micturition reflex but one wishes to go anyway Valsalva maneuver used to compress bladder Excites stretch receptors early to get the reflex started

122 Renal Insufficiency and Hemodialysis Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Thermometer Dialysis tubing Dialysis fluid Artery Vein Shunt Blood pump To drain Figure Hank Morgan/Photo Researchers, Inc. Bubble trap Cutaway view of dialysis chamber Flow meter