Implementation of an ex-vivo Th17 Assay
|
|
|
- Thomas Preston
- 5 years ago
- Views:
Transcription
1 Implementation of an ex-vivo Th17 Assay Dr Laine Hosking Immunology Laboratory, RCH
2 Background Expanding roles for CD4 + T cells in immunity to viruses Susan L. Swain, K. Kai McKinstry & Tara M. Strutt Nature Reviews Immunology 12, (February 2012) doi: /nri3152
3 T-Helper Type 17 Cells (Th17) Known to play a key role in mucosal immunity Differentiation is promoted mainly by dendritic cells secreting IL-1, IL-6 and TGF-β activate transcription factors STAT3 and RORγt IL-17 production IL-17 Induction of proinflammatory cytokines & chemokines Recruitment of neutrophils Distinguished from other CD4 T cells by receptors and IL-17 production
4 However
5 Chronic Mucocutaneous Candidiasis Heterogeneous group of disorders characterised by recurrent/persistent superficial infections of the skin, mucous membranes & nails with Candida organisms Susceptibility has been shown in patients with impaired Th17 development and function PIDs associated with CMC Decreased Th17 production STAT 3 LOF STAT 1 GOF IL12 and IL12RB1 deficiencies Autoantibodies against IL-17 AIRE mutations
6 AIRE mutations Curr Opin Allergy Clin Immunol Dec; 12(6):
7 Aim To implement a whole blood one-tube flow cytometric assay for the rapid identification of Th17 deficient patients; to identify patients with possible PID as cause of CMC and patients with suspected AD-HIES Modified protocol by Dhalla et al. (Clin. Exp. Immunol. 2016) who compared an in-vitro assay for Th17 cells using intracellular cytokine staining for IL-17A with an ex-vivo assay using cell surface markers CCR6+CXCR3 CCR4+CD161+
8 Method Th17 cells defined by their surface markers: CD45+CD3+CD4+CD45RA-CCR6+CXCR3- Cell surface markers CD45 KrO CD3 PCB CD4 FITC CD45RA PC7 CCR6 AF647 CXCR3 PE 100ul whole blood 30 minutes assay Acquired on Gallios Flow Cytometer Collect 20,000 CD4 T cell events Run in parallel to healthy control Results expressed as Th17 as percentage of CD4 T cells
9 Results 19 individual healthy controls have been assessed Th17 cells account for % of CD4+ T cells (5 th -95 th centile), mean 7.8% % of CD4+CD45RA- T cells (5 th -95 th centile) 3 molecularly defined PID patients 2 STAT 1 GOF 1 STAT 3 LOF
10 Healthy Control CD4 T Cells
11 STAT 3 Loss of Function 19yo male with Hyper-IgE syndrome (STAT3 LOF) Chronic Aspergillus infection with pneumatocoeles No CMC Th17 0.3% of CD4 T cells, and 1.9% of CD4+CD45RA- T cells
12 STAT 1 Gain of Function CC 42yo male with STAT1 GOF Th17 0.7% CD4 T cells, 1.34% of CD4+CD45RA- T cells Note if only assessing for CCR6+ cells this patient would look normal
13 STAT 1 GOF 5yo female with STAT 1 GOF CMC & bronchiectasis Th17 0.0% of CD4 T cells and 0.1% of CD4+CD45RA- T cells Quite a difference compared with adult STAT 1 patient for CCR6+ population
14 Clinical Utility All CMC patients run to date have lower Th17 as compared to healthy controls Cost effective & rapid flow assay that can be used as a screening tool in patients with chronic mucocutaneous candidiasis or AD-HIES Whole blood Set up within 24hrs Alternative method involves stimulation and intracellular staining Help target genetic testing where indicated
15 Challenges Determining what is normal for the paediatric population Lower memory CD4 T cells, therefore with reduced Th17 cells (as % of CD4)
16 References Dhalla F, Fox H, Davenport EE, et al. Chronic mucocutaneous candidiasis: characterization of a family with STAT 1 gain of function and development of an ex vivo assay for Th17 deficiency of diagnostic utility. Clinical and Experimental Immunology. 2016;184(2): doi: /cei Engelhardt KR, Grimbacher B. Mendelian traits causing susceptibility to mucocutaneous fungal infections in human subjects. J Allergy Clin Immunol 2012; 129: ; quiz 6 7 Iwakura Y, Ishigame H, Saijo S, Nakae S. Functional specialization of interleukin-17 family members. Immunity Feb 25;34(2): Puel A, Cypowyj S, Maródi L, Abel L, Picard C, Casanova J-L. Inborn errors of human IL-17 immunity underlie chronic mucocutaneous candidiasis. Current opinion in allergy and clinical immunology. 2012;12(6): doi: /aci.0b013e328358cc0b. Susan L. Swain, K. Kai McKinstry & Tara M. Strutt. Expanding roles for CD4+ T cells in immunity to viruses. Nature Reviews Immunology 12, (February 2012) doi: /nri3152
17 Thank You & Questions
CD4+ T Helper T Cells, and their Cytokines in Immune Defense and Disease
CD4+ T Helper T Cells, and their Cytokines in Immune Defense and Disease Andrew Lichtman M.D., Ph.D. Brigham and Women s Hospital Harvard Medical School Lecture outline Intro to T cell mediated immunity
Allergy and Immunology Review Corner: Chapter 13 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti, MD.
Allergy and Immunology Review Corner: Chapter 13 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti, MD. Chapter 13: Mechanisms of Immunity to Viral Disease Prepared by
CD4 + T Helper T Cells. and their cytokines in immune defense and disease. Types of T Cell Mediated Immune Reactions
CD4 + T Helper T Cells. and their cytokines in immune defense and disease Andrew Lichtman M.D., Ph.D. Brigham and Women s Hospital Harvard Medical School BWH HMS Types of T Cell Mediated Immune Reactions
CD4 + T Helper T Cells. and their cytokines in immune defense and disease. Types of T Cell Mediated Immune Reactions
CD4 + T Helper T Cells. and their cytokines in immune defense and disease Andrew Lichtman M.D., Ph.D. Brigham and Women s Hospital Harvard Medical School BWH HMS Types of T Cell Mediated Immune Reactions
DURACLONE IF BE CERTAIN ABOUT THE RESPONSE. l res. a il n c n. For Research Use Only - Not for use in Diagnostic procedures
DURACLONE IF earch tria l res lc a om ic il n c n nio pa Yo ur BE CERTAIN ABOUT THE RESPONSE For Research Use Only - Not for use in Diagnostic procedures BE CERTAIN ABOUT THE RESPONSE The sensitive and
Effector T Cells and
1 Effector T Cells and Cytokines Andrew Lichtman, MD PhD Brigham and Women's Hospital Harvard Medical School 2 Lecture outline Cytokines Subsets of CD4+ T cells: definitions, functions, development New
Effector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells
ICI Basic Immunology course Effector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells Abul K. Abbas, MD UCSF Stages in the development of T cell responses: induction
Naive, memory and regulatory T lymphocytes populations analysis
Naive, memory and regulatory T lymphocytes populations analysis Jaen Olivier, PhD ojaen@beckmancoulter.com Cellular Analysis application specialist Beckman Coulter France Introduction Flow cytometric analysis
USTEKINUMAB and BRIAKINUMAB
USTEKINUMAB and BRIAKINUMAB Andrew Blauvelt, M.D. Professor, Dept. of Dermatology and Dept. of Molecular Microbiology & Immunology Oregon Health & Science University Chief, Dermatology Service Veteran
Autoimmunity and Primary Immune Deficiency
Autoimmunity and Primary Immune Deficiency Mark Ballow, MD Division of Allergy & Immunology USF Morsani School of Medicine Johns Hopkins All Children s Hospital St Petersburg, FL The Immune System What
ﺖاﻀﻴﺒﻤﻠا ﺾﻴﺒﻠا ﻦﻤزﻤﻠا ﻰﻠﻋ ﺔﻴﺸﻐأﻠا ﺔﻴﻄاﺨﻤﻠا
اﻠﻤﺨاﻄﻴﺔ اﻠأﻐﺸﻴﺔ ﻋﻠﻰ اﻠﻤزﻤﻦ اﻠﺒﻴﺾ اﻠﻤﺒﻴﻀاﺖ داء= candidiasis_chronic_mucocutaneos 1 / 19 اﻠﻤﺨاﻄﻴﺔ اﻠأﻐﺸﻴﺔ ﻋﻠﻰ اﻠﻤزﻤﻦ اﻠﺒﻴﺾ اﻠﻤﺒﻴﻀاﺖ داء= candidiasis_chronic_mucocutaneos 2 / 19 Chronic Mucocutaneous 3 /
Chapter 10 (pages ): Differentiation and Functions of CD4+ Effector T Cells Prepared by Kristen Dazy, MD, Scripps Clinic Medical Group
FIT Board Review Corner September 2015 Welcome to the FIT Board Review Corner, prepared by Andrew Nickels, MD, and Sarah Spriet, DO, senior and junior representatives of ACAAI's Fellows-In-Training (FITs)
Tolerance, autoimmunity and the pathogenesis of immunemediated inflammatory diseases. Abul K. Abbas UCSF
Tolerance, autoimmunity and the pathogenesis of immunemediated inflammatory diseases Abul K. Abbas UCSF Balancing lymphocyte activation and control Activation Effector T cells Tolerance Regulatory T cells
Tolerance 2. Regulatory T cells; why tolerance fails. Abul K. Abbas UCSF. FOCiS
1 Tolerance 2. Regulatory T cells; why tolerance fails Abul K. Abbas UCSF FOCiS 2 Lecture outline Regulatory T cells: functions and clinical relevance Pathogenesis of autoimmunity: why selftolerance fails
IMMUNODEFICIENCIES CLASSIFICATION OF PIDS PRIMARY IMMUNODEFICIENCIES CLASSIFIED?
PRIMARY IMMUNODEFICIENCIES CLASSIFICATION OF PIDS HOW ARE PRIMARY IMMUNODEFICIENCIES CLASSIFIED? 1 PRIMARY IMMUNODEFICIENCIES KEY ABBREVIATIONS CGD CVID IBD IgA/D/E/G/M IPOPI PID SCID Chronic granulomatous
T cell-mediated immunity
T cell-mediated immunity Overview For microbes within phagosomes in phagocytes.cd4+ T lymphocytes (TH1) Activate phagocyte by cytokines studies on Listeria monocytogenes For microbes infecting and replicating
When should a Primary Immunodeficiency be Suspected?
When should a Primary Immunodeficiency be Suspected? Ricardo U Sorensen. MD Head, Jeffrey Modell Diagnostic Center for Primary Immunodeficiencies. LSUHSC, New Orleans Learning Objectives Define Primary
Autoimmune Diseases. Betsy Kirchner CNP The Cleveland Clinic
Autoimmune Diseases Betsy Kirchner CNP The Cleveland Clinic Disclosures (financial) No relevant disclosures Learning Objectives Explain the pathophysiology of autoimmune disease Discuss safe administration
Original Article Serum Levels of IL-17A Increase in Asthma But Don t Correlate with Serum Level of IgE and Asthma Severity
International Journal of Medical Laboratory 2015;2(1):25-33. Original Article Serum Levels of IL-17A Increase in Asthma But Don t Correlate with Serum Level of IgE and Asthma Severity Masouma Mowahedi
Biology of Immune Aging
Biology of Immune Aging Jorg J. Goronzy Stanford University Immune deficiency Increase morbidity and mortality from infections Poor vaccine responses Cancer Immune Aging Chronic inflammation Coronary artery
CYTOKINES. Based on: Cellular and Molecular Immunology, 4 th ed.,abbas A.K., Lichtman A.H. and Pober J.S. Sounders company; Philadelphia, 2010.
CYTOKINES Based on: Cellular and Molecular Immunology, 4 th ed.,abbas A.K., Lichtman A.H. and Pober J.S. Sounders company; Philadelphia, 2010. 1 What are cytokines? Glycoproteins (15 25 kda): Interleukins
The central role of T helper cells
e central role of T helper s the expanding universe of lineages Cytometric analysis of T function Hyun-Dong Chang Deutsches Rheuma-Forschungszentrum Berlin, a Leibniz Institute, Berlin, Germany CD8+ T
I. Critical Vocabulary
I. Critical Vocabulary A. Immune System: a set of glands, tissues, cells, and dissolved proteins that combine to defend against non-self entities B. Antigen: any non-self chemical that triggers an immune
Following T-cell activation and differentiation with HTRF reagents: IL-2, IFN-γ and IL-17
Following T-cell activation and differentiation with HTRF reagents: IL-2, IFN-γ and IL-17 4 th HTRF Symposium for Drug Discovery Avignon, Sept. 24-26, 28 Introduction: T-cells have effector and helper
Cytokines modulate the functional activities of individual cells and tissues both under normal and pathologic conditions Interleukins,
Cytokines http://highered.mcgraw-hill.com/sites/0072507470/student_view0/chapter22/animation the_immune_response.html Cytokines modulate the functional activities of individual cells and tissues both under
Chapter 24 The Immune System
Chapter 24 The Immune System The Immune System Layered defense system The skin and chemical barriers The innate and adaptive immune systems Immunity The body s ability to recognize and destroy specific
Monocyte subsets in health and disease. Marion Frankenberger
Monocyte subsets in health and disease Marion Frankenberger main cellular components: Leukocytes Erythrocytes Composition of whole blood Monocytes belong to the cellular components of peripheral blood
Immunodeficiency and Skin (September 21, 2018) By (Arti Nanda, MD, DNBE [Kuwait])
![Immunodeficiency and Skin (September 21, 2018) By (Arti Nanda, MD, DNBE [Kuwait])](/thumbs/90/103062332.jpg "Immunodeficiency and Skin (September 21, 2018) By (Arti Nanda, MD, DNBE [Kuwait])")
Immunodeficiency and Skin (September 21, 2018) By (Arti Nanda, MD, DNBE [Kuwait]) Immune deficiency refers to a state in which part of immune system is missing or defective resulting into an inability
Cell-mediated Immunity
Cellular & Molecular Immunology Cell-mediated Immunity Nicholas M. Ponzio, Ph.D. Department of Pathology & Laboratory Medicine April 6, 2009 Today s Presentation: Overview Cellular Interactions In Humoral
B cell activation and antibody production. Abul K. Abbas UCSF
1 B cell activation and antibody production Abul K. Abbas UCSF 2 Lecture outline B cell activation; the role of helper T cells in antibody production Therapeutic targeting of B cells 3 Principles of humoral
Chapter 3, Part A (Pages 37-45): Leukocyte Migration into Tissues
Allergy and Immunology Review Corner: Chapter 3, Part A (pages 37-45) of Cellular and Molecular Immunology (Seventh Edition), by Abul K. Abbas, Andrew H. Lichtman and Shiv Pillai. Chapter 3, Part A (Pages
IMMUNOTOOLS: EFFECT OF NOTCH-DEFICIENT MACROPHAGES TO AUTOIMMUNE DISEASE WIPAWEE WONGCHANA
IMMUNOTOOLS: EFFECT OF NOTCH-DEFICIENT MACROPHAGES TO AUTOIMMUNE DISEASE 22-02-2017 WIPAWEE WONGCHANA WHAT DO YOU SEE? Allergy Ref: http://carrington.edu/blog/medical/vaccines/smallpox-andsmallpox-vaccine/
Question 1. Kupffer cells, microglial cells and osteoclasts are all examples of what type of immune system cell?
Abbas Chapter 2: Sarah Spriet February 8, 2015 Question 1. Kupffer cells, microglial cells and osteoclasts are all examples of what type of immune system cell? a. Dendritic cells b. Macrophages c. Monocytes
DNA vaccine, peripheral T-cell tolerance modulation 185
Subject Index Airway hyperresponsiveness (AHR) animal models 41 43 asthma inhibition 45 overview 41 mast cell modulation of T-cells 62 64 respiratory tolerance 40, 41 Tregs inhibition role 44 respiratory
T-Cell Research. Novel multicolor flow cytometry tools for the study of CD4 + T-cell differentiation and plasticity
T-Cell Research Novel multicolor flow cytometry tools for the study of CD4 + T-cell differentiation and plasticity A Solid Commitment to Research: Flexible Ways to Study CD4 T-Cell Differentiation and
Airway Inflammation in Asthma Chih-Yung Chiu 1,2, Kin-Sun Wong 2 1 Department of Pediatrics, Chang Gung Memorial Hospital, Keelung, Taiwan.
REVIEW ARTICLE Chih-Yung Chiu 1,2, Kin-Sun Wong 2 1 Department of Pediatrics, Chang Gung Memorial Hospital, Keelung, Taiwan. 2 Division of Pediatric Pulmonology, Department of Pediatrics, Chang Gung Memorial
A. Incorrect! The duodenum drains to the superior mesenteric lymph nodes. B. Incorrect! The jejunum drains to the superior mesenteric lymph nodes.
USMLE Step 1 Problem Drill 11: Immunology Question No. 1 of 10 1. A 67 year old man is discovered to have metastatic disease involving his inferior mesenteric lymph nodes. His primary cancer is most likely
DURACLONE RE THERE ARE EVENTS YOU CANNOT AFFORD TO MISS
DURACLONE RE THERE ARE EVENTS YOU CANNOT AFFORD TO MISS Your clinical research trial companion For Reseach Use Only - Not for use in Diagnostic procedures THERE ARE EVENTS YOU CANNOT AFFORD TO MISS Rare
Part III Innate and Adaptive Immune Cells: General Introduction
Innate and Adaptive Immune Cells: General Introduction Iván López-Expósito As an organ specialized in food digestion and nutrient absorption, the intestinal mucosa presents a huge surface area (almost
Candida albicans and Candida parapsilosis Induce Different T-Cell Responses in Human Peripheral Blood Mononuclear Cells
MAJOR ARTICLE Candida albicans and Candida parapsilosis Induce Different T-Cell Responses in Human Peripheral Blood Mononuclear Cells Adél Tóth, 1 Katalin Csonka, 1 Cor Jacobs, 2 Csaba Vágvölgyi, 1 Joshua
PIDPID GLOSSARYID GLOSSARY
PIDPID GLOSSARYID GLOSSARY PID GLOSSARY Acquired immune deficiency Acquired Immune Deficiency Syndrome Acute Adenosine Deaminase(ADA) Agammaglobulinemia Amniocentesis Anemia Antibody Antigen Aspergillus
Innate immune regulation of T-helper (Th) cell homeostasis in the intestine
Innate immune regulation of T-helper (Th) cell homeostasis in the intestine Masayuki Fukata, MD, Ph.D. Research Scientist II Division of Gastroenterology, Department of Medicine, F. Widjaja Foundation,
Immune response to pathogens
Bari, May 26, 2017 Immune response to pathogens Francesco Dieli Department of Biopathology and Medical Biotechnologies Central Laboratory of Advanced Diagnosis and Biomedical Research University of Palermo
IL-12 family members in experimental colitis. Markus F. Neurath I. Medical Clinic Johannes Gutenberg-University Mainz, Germany
IL-12 family members in experimental colitis Markus F. Neurath I. Medical Clinic Johannes Gutenberg-University Mainz, Germany IBD - Pathogenesis Genetic Predisposition Bacterial Antigens Activation of
IL-17 in health and disease. March 2014 PSO13-C051n
IL-17 in health and disease March 2014 PSO13-C051n Originally Researchers Suggested That IL-12 and IL-4 drove Th Cell Differentiation Naïve CD4 + T cell Question: Which of these cell types is responsible
Activation and migration of CD4 + lymphocytes induced by different foreign bodies
Activation and migration of CD4 + lymphocytes induced by different foreign bodies Бобрышев Д. В., F. Entschladen 2, В.Д. Гримм, W.-D. Grimm 1 Ставрополь State Medical University, Россия, University of
Lecture outline. Immunological tolerance and immune regulation. Central and peripheral tolerance. Inhibitory receptors of T cells. Regulatory T cells
1 Immunological tolerance and immune regulation Abul K. Abbas UCSF 2 Lecture outline Central and peripheral tolerance Inhibitory receptors of T cells Regulatory T cells 1 The immunological equilibrium:
Fluorochrome Panel 1 Panel 2 Panel 3 Panel 4 Panel 5 CTLA-4 CTLA-4 CD15 CD3 FITC. Bio) PD-1 (MIH4, BD) ICOS (C398.4A, Biolegend) PD-L1 (MIH1, BD)
Additional file : Table S. Antibodies used for panel stain to identify peripheral immune cell subsets. Panel : PD- signaling; Panel : CD + T cells, CD + T cells, B cells; Panel : Tregs; Panel :, -T, cdc,
FOR OPTIMAL GUT HEALTH KEMIN.COM/GUTHEALTH
FOR OPTIMAL GUT HEALTH KEMIN.COM/GUTHEALTH ALETA A SOURCE OF 1,3-BETA GLUCANS Aleta is highly bioavailable, offering a concentration greater than 5% of 1,3-beta glucans. Aleta provides a consistent response
The Immune System. These are classified as the Innate and Adaptive Immune Responses. Innate Immunity
The Immune System Biological mechanisms that defend an organism must be 1. triggered by a stimulus upon injury or pathogen attack 2. able to counteract the injury or invasion 3. able to recognise foreign
T H 17 deficiency in human disease
Clinical reviews in allergy and immunology Series editors: Donald Y. M. Leung, MD, PhD, and Dennis K. Ledford, MD T H 17 deficiency in human disease Douglas R. McDonald, MD, PhD Boston, Mass INFORMATION
Chemical aspects of the cell. Chemicals that control cell signaling: chemotaxis
Chemical aspects of the cell Chemicals that control cell signaling: chemotaxis Cellular responses Chemotaxis Cellular response to an environmental substance with a directional movement. Chemokinesis Cellular
Diagnosis and Management of Fungal Allergy Monday, 9-139
Diagnosis and Management of Fungal Allergy Monday, 9-139 13-2010 Alan P. Knutsen,, MD Director, Pediatric Allergy & Immunology Director, Jeffrey Modell Diagnostic Center for Primary Immunodeficiencies
Antigen Presentation and T Lymphocyte Activation. Abul K. Abbas UCSF. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Abul K. Abbas UCSF FOCiS 2 Lecture outline Dendritic cells and antigen presentation The role of the MHC T cell activation Costimulation, the B7:CD28 family
Mendelian traits causing susceptibility to mucocutaneous fungal infections in human subjects
Mechanisms of allergic diseases Series editors: Joshua A. Boyce, MD, Fred Finkelman, MD, William T. Shearer, MD, PhD, and Donata Vercelli, MD Mendelian traits causing susceptibility to mucocutaneous fungal
Bead Based Assays for Cytokine Detection
Bead Based Assays for Cytokine Detection September 27, 2014 6 th EFIS-EJI South East European Immunology School SEEIS 2014 Timisoara, Romania The Cells of the Immune System The Immune Reaction (Th2) (Th1)
5/9/2017. Another Itchy Dog. Skin Diseases are Common. Rule Out Infectious Causes of Itching Prior to Diagnosing Allergy. 2011: VPI Pet Insurance 1
Another Itchy Dog New Understanding of the Pathophysiology of Canine Allergic Disease Dr. Kimberly Greene- DVM, PSV Midwest Skin Diseases are Common 2011: VPI Pet Insurance 1 1. 2. 3. Ear Infections Skin
Helminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii. Ringworm fungus HIV Influenza
Helminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii Ringworm fungus HIV Influenza Candida Staph aureus Mycobacterium tuberculosis Listeria Salmonella Streptococcus Levels
Updates on Candida Immunology
2018 MSGERC Biennial Meeting Plenary Lectures on Candida September 26, 2018 Updates on Candida Immunology Michail S. Lionakis, MD, ScD Chief, Fungal Pathogenesis Section Laboratory of Clinical Immunology
Recurrent Infection, Pulmonary Disease, and Autoimmunity as Manifestations of Immune Deficiency
Recurrent Infection, Pulmonary Disease, and Autoimmunity as Manifestations of Immune Deficiency Erwin W. Gelfand, M.D. Professor, Department of Pediatrics National Jewish Health Professor of Immunology
Relevant Disclosures
6/18/215 Therapeutic developments for autoimmune demyelinating diseases: Musings from a MD (Mouse Doctor) Michael K. Racke, M.D. May 28, 215 Relevant Disclosures Editorial Boards for Journal of Neuroimmunology,
Analysis of regulatory T cell subsets in the peripheral blood of immunoglobulin A nephropathy (IgAN) patients
Analysis of regulatory T cell subsets in the peripheral blood of immunoglobulin A nephropathy (IgAN) patients S. Yang, B. Chen, J. Shi, F. Chen, J. Zhang and Z. Sun Department of Nephrology, Huaihe Hospital
Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12
1 Supplementary Data Figure legends Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12 serum levels measured by multiplex ELISA (Luminex) in FL patients before
The CAPRI-T was one of two (along with the CAPRI-NK, see reference. [29]) basic immunological studies nested within the CAMELIA trial.
![The CAPRI-T was one of two (along with the CAPRI-NK, see reference. [29]) basic immunological studies nested within the CAMELIA trial.](/thumbs/88/117921481.jpg "The CAPRI-T was one of two (along with the CAPRI-NK, see reference. [29]) basic immunological studies nested within the CAMELIA trial.")
1 SUPPLEMENTARY INFORMATION Patient description and recruitment The CAPRI-T was one of two (along with the CAPRI-NK, see reference [29]) basic immunological studies nested within the CAMELIA trial. Patients
Concepts in Rhinosinusitis. Nick Jones University of Nottingham
Concepts in Rhinosinusitis Nick Jones University of Nottingham Definition of rhinosinusitis Very broad and is based on clinical symptoms, CT and endoscopy NOT on pathology Definition of rhinosinusitis
Defects of Innate Immunity
Defects of Innate Immunity TLR signalling IRAK4 and MyD88 AR Same clinical phenotype for both genes (Recurrent) invasive bacterial infections: Meningitis and septicemia >> skin Pneumococcus >> Staph aureus
Kerrie Clerici, Michael Swain, Dominic Fernandez, Julia Schulz, Matthew Archer, Janine Campbell
Minimal Residual Disease (MRD) Testing by Flow Cytometry for childhood Precursor B Cell Acute Lymphoblastic Leukaemia Royal Children s Hospital experience. Kerrie Clerici, Michael Swain, Dominic Fernandez,
Review Questions: Janeway s Immunobiology 8th Edition by Kenneth Murphy
Review Questions: Janeway s Immunobiology 8th Edition by Kenneth Murphy Chapter 11 (pages 429-460): Dynamics of Adaptive Immunity prepared by Kelly von Elten, Walter Reed National Military Medical Center,
CLINICAL USE OF CELLULAR SUBPOPULATION ANALYSIS IN BM
CLINICAL USE OF CELLULAR SUBPOPULATION ANALYSIS IN BM CANCER RESEARCH CENTRE, UNIVERSITY AND UNIVERSITY HOSPITAL OF SALAMANCA (SPAIN)( Sao Paulo, 18th of April, 2009 IDENTIFICATION OF HPC (I) 1.- In vivo
Intrinsic cellular defenses against virus infection
Intrinsic cellular defenses against virus infection Detection of virus infection Host cell response to virus infection Interferons: structure and synthesis Induction of antiviral activity Viral defenses
Immunology for the Rheumatologist
Immunology for the Rheumatologist Rheumatologists frequently deal with the immune system gone awry, rarely studying normal immunology. This program is an overview and discussion of the function of the
Immunology MIMM-314 MID-TERM II EXAMINATION. 1 hour between 8:30 a.m. - 10:00 a.m. McIntyre Medical Rm 504 (Martin Amphitheatre)
GROUP (Version) 1 Annotated version April 8, 2011, RGEP DEPARTMENT OF MICROBIOLOGY AND IMMUNOLOGY Immunology MIMM-314 MID-TERM II EXAMINATION Course Coordinator: Dr. Roger Palfree Date: Thursday, March
A. Incorrect! It s not correct. Synergism of cytokines refers to two or more cytokines acting together.
Immunology - Problem Drill 11: Cytokine and Cytokine Receptors Question No. 1 of 10 1. A single cytokine can act on several different cell types, which is known as. Question #1 (A) Synergism (B) Pleiotropism
Generation of post-germinal centre myeloma plasma B cell.
Generation of post-germinal centre myeloma. DNA DAMAGE CXCR4 Homing to Lytic lesion activation CD38 CD138 CD56 Phenotypic markers Naive Secondary lymphoid organ Multiple myeloma is a malignancy of s caused
Acquired Immunity 2. - Vaccines & Immunological Memory - Wataru Ise. WPI Immunology Frontier Research Center (IFReC) Osaka University.
Acquired Immunity 2 - Vaccines & Immunological Memory - Wataru Ise WPI Immunology Frontier Research Center (IFReC) Osaka University Outline 1. What is vaccine (vaccination)? 2. What is immunological memory?
Chapter 13: Cytokines
Chapter 13: Cytokines Definition: secreted, low-molecular-weight proteins that regulate the nature, intensity and duration of the immune response by exerting a variety of effects on lymphocytes and/or
Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal-dominant gain-of-phosphorylation STAT1 mutations
Title Author(s) Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal-dominant gain-of-phosphorylation STAT1 mutations Lee, PPW; Mao, H; Yang, W; Chan, KW; Ho, MHK; Lee,
CONTRACTING ORGANIZATION: Johns Hopkins University School of Medicine Baltimore, MD 21205
AD Award Number: DAMD7---7 TITLE: Development of Artificial Antigen Presenting Cells for Prostate Cancer Immunotherapy PRINCIPAL INVESTIGATOR: Jonathan P. Schneck, M.D., Ph.D. Mathias Oelke, Ph.D. CONTRACTING
NTD Vaccine Design Toolkit and Training Workshop Providence, RI January 05, 2011 Cytokines Leslie P. Cousens, PhD EpiVax, Inc.
NTD Vaccine Design Toolkit and Training Workshop Providence, RI January 05, 2011 Cytokines Leslie P. Cousens, PhD EpiVax, Inc. Cytokines Properties of Cytokines Cytokines are proteins with specific roles
Prof. Sandrine Florquin Department of Pathology Academic Medical Center University of Amsterdam Amsterdam, The Netherlands. Slide 1.
Interleukin 17 in renal biopsies as risk factor for progression Sandrine Florquin, Amsterdam, The Netherlands Chairs: Mohamed R. Daha, Leiden, The Netherlands Pierre Ronco, Paris, France Prof. Sandrine
Determinants of Immunogenicity and Tolerance. Abul K. Abbas, MD Department of Pathology University of California San Francisco
Determinants of Immunogenicity and Tolerance Abul K. Abbas, MD Department of Pathology University of California San Francisco EIP Symposium Feb 2016 Why do some people respond to therapeutic proteins?
Attribution: University of Michigan Medical School, Department of Microbiology and Immunology
Attribution: University of Michigan Medical School, Department of Microbiology and Immunology License: Unless otherwise noted, this material is made available under the terms of the Creative Commons Attribution
Activation of inflammatory response by fungal cell wall components and toxins
Finnish Institute of Occupational Health Activation of inflammatory response by fungal cell wall components and toxins Sampsa Matikainen Innate Immunity Research Group Unit of Immunotoxicology Finnish
Cellular Immune response. Jianzhong Chen, Ph.D Institute of immunology, ZJU
Cellular Immune response Jianzhong Chen, Ph.D Institute of immunology, ZJU Concept of adaptive immune response T cell-mediated adaptive immune response I. Concept of immune response A collective and coordinated
Tolerance 2. Regulatory T cells; why tolerance fails. FOCiS. Lecture outline. Regulatory T cells. Regulatory T cells: functions and clinical relevance
1 Tolerance 2. Regulatory T cells; why tolerance fails Abul K. Abbas UCSF FOCiS 2 Lecture outline Regulatory T cells: functions and clinical relevance Pathogenesis of autoimmunity: why selftolerance fails
Optimizing Intracellular Flow Cytometry
Optimizing Intracellular Flow Cytometry Detection of Cytokines, Transcription Factors, and Phosphoprotein by Flow Cytometry Presented by Erika O Donnell, PhD, BD Biosciences 23-14876-00 Outline Basic principles
Role of Th17 cells in the immunopathogenesis of dry eye disease
Role of Th17 cells in the immunopathogenesis of dry eye disease The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Chauhan,
Voting feature in App. Breaks Optional Event Educational Session Meet the expert INGID Working Party
TIMETABLE Plenary Workshop Special Breaks Optional Event Educational Meet the expert INGID Working Party Wednesday 24 October 2018 I 08:30-09:00 Educational Lecture - Lymphoproliferation in PIDs 09:00-09:30
Alessandra Franco, M.D., Ph.D. UCSD School of Medicine Department of Pediatrics Division of Allergy, Immunology and Rheumatology
Natural regulatory T cells recognize the heavy constant region (Fc) of immunoglobulins: a novel mechanism for IVIG immunotherapy in Pediatric Immune-mediated diseases Alessandra Franco, M.D., Ph.D. UCSD
THE ROLE OF IL-17 IN THE INNATE IMMUNE RESPONSE TO OROPHARYNGEAL CANDIDIASIS. Alanna Carol Peterson
THE ROLE OF IL-17 IN THE INNATE IMMUNE RESPONSE TO OROPHARYNGEAL CANDIDIASIS by Alanna Carol Peterson B.S. Biological Sciences, San Jose State University, 2007 B.A. English, University of California at
Central tolerance. Mechanisms of Immune Tolerance. Regulation of the T cell response
Immunoregulation: A balance between activation and suppression that achieves an efficient immune response without damaging the host. Mechanisms of Immune Tolerance ACTIVATION (immunity) SUPPRESSION (tolerance)
Mechanisms of Immune Tolerance
Immunoregulation: A balance between activation and suppression that achieves an efficient immune response without damaging the host. ACTIVATION (immunity) SUPPRESSION (tolerance) Autoimmunity Immunodeficiency
Simultaneous correlation of cytokine production with Treg and Th17 cell proliferation
Simultaneous correlation of cytokine production with Treg and Th17 cell proliferation Jurg Rohrer, PhD Director, R&D BD Biosciences 23-11773-00 For Research Use Only. Not for use in diagnostic or therapeutic
Chapter 11 CYTOKINES
Chapter 11 CYTOKINES group of low molecular weight regulatory proteins secreted by leukocytes as well as a variety of other cells in the body (8~30kD) regulate the intensity and duration of the immune
Understanding basic immunology. Dr Mary Nowlan
Understanding basic immunology Dr Mary Nowlan 1 Immunology Immunology the study of how the body fights disease and infection Immunity State of being able to resist a particular infection or toxin 2 Overview
Medical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University
Medical Virology Immunology Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University Human blood cells Phases of immune responses Microbe Naïve
Supplemental Information. T Cells Enhance Autoimmunity by Restraining Regulatory T Cell Responses via an Interleukin-23-Dependent Mechanism
Immunity, Volume 33 Supplemental Information T Cells Enhance Autoimmunity by Restraining Regulatory T Cell Responses via an Interleukin-23-Dependent Mechanism Franziska Petermann, Veit Rothhammer, Malte
Dr Rodney Itaki Lecturer Division of Pathology Anatomical Pathology Discipline
Pathology of Asthma Dr Rodney Itaki Lecturer Division of Pathology Anatomical Pathology Discipline Bronchial Asthma Definition: chronic, relapsing inflammatory lung disorder characterised by reversible
Immunological Changes in the FRT During the Menstrual Cycle
Immunological Changes in the FRT During the Menstrual Cycle Charles R. Wira PhD Department of Physiology and Neurobiology Geisel School of Medicine at Dartmouth Lebanon, NH 03756 May 1, 2014 Anatomy and
The recruitment of leukocytes and plasma proteins from the blood to sites of infection and tissue injury is called inflammation
The migration of a particular type of leukocyte into a restricted type of tissue, or a tissue with an ongoing infection or injury, is often called leukocyte homing, and the general process of leukocyte