March Immunoglobulins, Total (IgA, IgE, IgG, IgM) Interleukin-6 (IL6) Protein Electrophoresis, CSF. Thyrotropin Receptor Antibody
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1 University of Washington March 2012 TEST CHANGE Alpha 1-Antitrypsin Anti-IgE Receptor Antibody Anti-Mullerian Hormone Aquaporin-4 Receptor Antibody ColoSeq - Polyposis Complement testing, C3, C4 Everolimus Fungal Serology Testing Aspergillus Antibody by CF and ID Blastomyces Antibody by CF and ID Coccidioides Antibody by CF Coccidioides Antibody by CF and ID Fungal Antibody Panel, CSF Histoplasma Antibody by CF and ID Haptoglobin Immunoglobulins, Total (IgA, IgE, IgG, IgM) Interleukin-6 (IL6) Protein Electrophoresis, CSF Stool Leukocyte Exam Thyrotropin Receptor Antibody Off Line Specimen Handling Alpha 1-Antitrypsin Pediatric: 2 plain Microtainers. Also Acceptable: Green, PST or Lavender tubes. A1AT Anti-IgE Receptor Antibody Specimen Collection: 3 ml blood in Red top. Allow blood to clot and centrifuge within one hour of collection. Centrifuge and aliquot serum into a plastic tube. Freeze at -20ºC. Unacceptable: Gel Separator tubes. Specimen Transport: 1 ml serum (min. 0.5 ml); Frozen After separation from cells: Ambient: Unacceptable; Refrigerated: 7 days; Frozen: 180 days. M, Thursday, results in 1-2 weeks Reference Range: % RFIGRG 88184, x2 Price: $153.40
2 Anti-Mullerian Hormone Specimen Collection: 1.5 ml blood in Gold SST tube. Red top tube also acceptable. Centrifuge and aliquot serum into plastic tube. Freeze serum. Specimen Transport: 0.5 ml (min. 0.2 ml); Frozen on ice. Unacceptable: Hemolyzed or lipemic specimens. Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 30 days (avoid repeated freeze/thaw cycles). M, W, F, results in 5 days Reference Range: Female 0-16 years: ng/ml years: ng/ml years: ng/ml years: ng/ml 50 years and older: ng/ml Male: 0-13 days: ng/ml 14 days-11 months: ng/ml 12 months-6 years: ng/ml 7-8 years: ng/ml 9-12 years: ng/ml years: ng/ml Adult males (17 and older): ng/ml RAMHG Price: $ Aquaporin-4 Receptor Antibody Specimen Collection: 3.0 ml blood in Gold SST is preferred, Red top tube is acceptable. Centrifuge and aliquot serum into plastic tube. Specimen Transport: 1.0 ml (min. 0.3 ml) Unacceptable: CSF, amniotic fluid, ocular fluid peritoneal fluid, synovial fluid, or plasma. Contaminated, hemolyzed, icteric, or lipemic specimens. After separation from cells: Ambient: 72 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles) Tuesday, results in 5 days Reference Range: Negative: 4 U/mL Indeterminate: 5 U/mL Positive: 6 U/mL Interpretive Data: Approximately 75% of patients with neuromyelitis optica (NMO) express antibodies to the aquaporin-4 (AQP4) receptor. Diagnosis of NMO requires the presence of longitudinally extensive acute myelitis (lesions extending over 3 or more vertebral segments) and optic neuritis. While absence of antibodies to the AQP4 receptor does not rule out the diagnosis of NMO, presence of this antibody is diagnostic for NMO. RAQP4G Price: $ ColoSeq - Polyposis This test uses next-generation sequencing to detect most mutations in APC and MUTYH. Mutations in these genes are associated with familial adenomatous polyposis syndrome (APC) and MUTYH-associated polyposis syndrome. The assay completely sequences all exons, introns, and flanking regions of these genes AND detects large deletions and duplications. For additional information, see: Test Information: Requisition: TestForms/genetics.pdf Specimen Collection: 10 ml whole blood in Lavender top tube tube. Also acceptable: ACD tube. Specimen Transport: Entire sample (min. 5 ml whole blood). Ship at room temperature for overnight delivery. OK to hold up to 7 days before shipping, if refrigerated. Once a month, results within 12 weeks. COSEQP CPT Codes: x32, x32, Price: $1,350 2 UW Laboratory Medicine March 2012
3 Complement testing, C3, C4 Pediatric: 2 plain Microtainers. Also Acceptable: Green, PST or Lavender tubes. Test Order Codes: C3, C4 Everolimus Specimen Collection: Specimen Transport: Reference Range: Price: $ ml blood in Lavender tube. Draw blood immediately before a schedule dose. 3 ml whole blood (min. 1.0 ml); After separation from cells: Ambient: 72 hours; Refrigerated: 14 days (preferred); Frozen: 14 days M - Sun., results in 2 days 3 to 8 ng/ml. REVERG Fungal Serology Testing Aspergillus Antibody by Complement Fixation and. Specimen Transport: 1 ml serum (min. 0.2 ml); Refrigerate RASPG x2 Price: $38.84 Blastomyces Antibody by Complement Fixation and Specimen Transport: 1 ml serum (min. 0.3 ml); Refrigerate RBLSG x2 Price: $54.24 Coccidioides Antibody by Complement Fixation or 3 ml CSF. Refrigerate Specimen Transport: 1 ml serum or CSF (min ml); Refrigerate RCOCFG Price: $26.03 Coccidioides Antibody by Complement Fixation and or 3 ml CSF. Refrigerate Specimen Transport: 1 ml serum or CSF (min ml); Refrigerate RCOCFG x2 Price: $37.06 Fungal Antibody Panel, CSF Includes Aspergillus, Blastomyces and Histoplasma (Yeast and Mycelia) Specimen Collection: 1 ml CSF. Specimen Transport: 1 ml CSF (min ml); Unacceptable: Grossly hemolyzed Reference Range: <1:2 RCFAP 86606, 86635, x2, Price: $
4 Histoplasma Antibody by Complement Fixation and Specimen Transport: 1 ml serum (min. 0.3 ml); Refrigerate RHISG x3 Price: $57.72 Haptoglobin Specimen Collection: 3 ml blood in RED TOP or GOLD SST tube preferred. Also acceptable: Lavender top (EDTA), Green top (Heparin), Lime Green PST or Pearl Top tube. HPT Immunoglobulins, Total (IgA, IgE, IgG, IgM) Also Acceptable: Green, PST or Lavender tubes. Test Order Codes: IGA, IGE, IGG, IGM Interleukin-6 (IL6) Specimen Collection: 3 ml blood in Red top tube or Gold SST. Specimen Transport: 1 ml serum (min ml); Ship frozen overnight, on Dry ice. After separation from cells: >24 hours frozen, stable 3 freeze/thaw cycles. M, W, F, results 3-5 days from receipt of specimen. Reference Range: <17.4 pg/ml. RIL6G Price: $ Protein Electrophoresis, CSF As of March 1, 2012, the Department of Laboratory Medicine will no longer perform testing for Protein Electrophoresis, CSF because of lack of demand for the test. We will forward requests for this test to Mayo Medical Laboratories, test #8274. You may choose to send this test directly to MAYO laboratory or another laboratory of your choice. Mayo Medical Laboratories 200 1st St SW Rochester, MN Stool Leukocyte Exam Specimen Collection: STOOL: in container with tight-fitting lid. If possible, please place parafilm or tape around the lid to prevent leakage during transportation. RECTAL SWAB: submit swab in transport medium. Specimen Transport: Unpreserved specimens need to arrive at the UWMC processing lab within 2 hours of collection. Stool leukocytes may be degraded in samples received more than two hours after collection, which may lead to false negative findings. In such instances, clinical correlation is advised. STOLEU Thyrotropin Receptor Antibody Specimen Collection: 3 ml blood in Red top tube preferred, Gold SST acceptable. Centrifuge and aliquot serum into a plastic tube. Specimen Transport: 1 ml serum (min. 0.5 ml); Refrigerate Unacceptable: Grossly hemolyzed specimen. After separation from cells: Refrigerated: 7 days (preferred); Frozen: 30 days M - F, results in 3 days. Reference Range: 1.75 IU/L. RTRABG Price: $ UW Laboratory Medicine March 2012
5 Online Test Guide: Fee Schedule: Call Reference Lab Services at Virology sletter: CONTACT US: Tel: web: March 2012 Reference Lab Services University of Washington PO Box Seattle, WA Non-Profit Org. U.S. Postage PAID Seattle, WA Permit No. 62 address service requested
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