Review the following alphabetical listing of our lab test catalog for each of our offered tests. Each page has at least the following elements:

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William Cobb
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1 Site: Fremont Rideout Health Group Laboratory Services Policy and Procedure Creation Date: 08/17/2009 Subject/Title: FRHG Laboratories' Catalog of Tests Q-S Document Owner: Corson, Karen Pagination: Page 1 of 9 Approved By and Title: Bill R. Pettross, M.D. Director of FRHG Laboratory Services Effective Date: Date of Revision/Review: Version #:1 Date of Next Review: 02/19/2011 Catalog Format: Review the following alphabetical listing of our lab test catalog for each of our offered tests. Each page has at least the following elements: Test Name LIS/HIS Code /Transportation Post Testing Collection Instructions Page 1 of 9

2 HIS Number Code/ LIS Code Reducing Substances Clinitest 1740, CLNTS /RMH Should be run at laboratory where specimen is collected Fresh urine or stool 10 ml 0.5 ml Negative Benedict's reaction Reducing sugars other than glucose will react positively with this test Specimen Handling Respiratory Syncytial Virus RSV 1462, RSVT Transport specimens to laboratory as soon as possible, where specimens should be processed immediately. Fresh nasopharyngeal washes, aspirates, and swabs, as well as tracheal aspirates Nasopharyngeal washings with volumes of 3 4 ml Nasopharyngeal swab with added transport media Collection of the nasopharyngeal washes is to be performed by the physician or nursing staff Negative EIA Excessively bloody specimens should not be tested. Specimens containing visible blood have been found to yield either uninterruptible or false-positive results. Please see the following page for a guide on proper collection of nasopharyngeal specimens Page 2 of 9

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4 Retic Count Reticulocyte Count 1197, RETIC RMH/ Performed at hospital where specimen drawn. EDTA whole blood (4.5 draw or 2.0 draw lavender tube or lavender top Microtainer acceptable) Heparinized blood is not acceptable 1 ml 100µL 24 hours at room temperature Adult: 0.5 to day-old: 0.9 to 1.6 Newborn: 0.5 to 6.0 Automated Instrumentation Rheumatoid Factor RA screen, RA, RF 1653/RFAC Run immediately in chemistry. Use hospital courier or cab to transport in a cooler to if needed. Heparinized Plasma or Serum 1 ml 0.25 ml 5 days at 2-8 C <15 IU/mL Latex Particle Immunoturbidmetric Page 4 of 9

5 Rotovirus Rotovirus stool antigen, Roto 1281, ROTO Store and transport at 2-8 o C Specimen Requirement 2 grams fresh stool in Sterile Screw Cap Container, 1 gram minimum Instructions Collect stool in sterile leak-proof container without media or preservative. A sample maybe collected into a clean dry container free of detergent residue, or obtained from a diaper. Rotavirus antigen not detected Enzyme Immunoassay Set-Up Schedule Processed ASAP, 7days a week, all shifts. : RPR RPR pre delivery, prenatal RPR 1662 &1744 (RPR pre delivery, mother's)1194 (RPR) RPR, PDRPR, RPRPD Sample should be tested within 48 hours. The sample may be stored at 2 to 8 C for five days The preferred sample is a 7.0 ml clot tube. 3.0 ml serum 1.0 ml serum Non-Reactive Flocculation Serum that is grossly hemolyzed or contaminated may not be used. A positive RPR will result in a confirmation being sent to the Sutter County Public Health Department. Page 5 of 9

6 Rubella Rubella Screen, German Measles 1550, RUBLA Serum should be separated and can be stored up to 48 hours at 2-8 C The preferred sample is a 7.0 ml clot tube 3.0 ml serum 1.0 ml serum Immune Latex agglutination Serum that displays excessive particulate matter, lipemia, or hemolysis could affect the test. Patients that are not pregnant that test as Non Immune can be vaccinated. Salicylate Aspirin, Acetylsalicylic Acid (ASA) 1201/SALICT /RMH Run immediately in chemistry. Heparinized Plasma or Serum 1 ml 0.25 ml 24 hours at 2-8 C mg/dl Homogeneous Enzyme Immunoassay (EMIT) Note last dose time and date with requisition in the LIS or HIS. Page 6 of 9

7 Semen Analysis-post vasectomy Post vasectomy sperm count Post vasectomy sperm count Post Vasectomy - SPPV Specimen should be received at room temperature, and processed immediately. Specimen Requirement Seminal fluid collected by masturbation Entire sample The physician ordering the test instructs the patient in the collection of the specimen. If the patient has questions he should be referred back to his physician Post vasectomy: no sperm seen Direct and microscopic observation.. Sickle Cell Prep 1207, SIKL Transport by courier to Whole Blood collected in EDTA, Citrate, or Heparin 1 ml 150 µl 2-8 C for up to two weeks. Negative Sickle Screen by Pacific Hemostasis Page 7 of 9

8 Sodium (Blood or Urine, Random) Na++ Blood: 1209/NA Urine, Random: 1208/UNAR /RMH Run immediately in chemistry. Heparinized Plasma or Serum or Urine, Random 1 ml 0.25 ml 5 days at 2-8 C Blood: mmol/l Urine, Random: No Range Ion Selective Electrode Specific Gravity Urine or Fluid Specific Gravity 1210, FSPG /RMH Test should be run at laboratory receiving sample. Specimen Requirement Urine or Fluid 10 ml 0.5 ml Refractometer Included in Urinanalysis. Streptozyme 1192, STZM Serum is separated from the cells and can be stored 24 hours at 2-8 C. 7.0 ml clot tube 3.0 ml of serum 1.0 ml of serum Negative Agglutination All positive Streptozymes will be sent to a reference lab for an ASO titer: LIS Code: ASO, HIS Number: 2376 Page 8 of 9

9 Specimen Container Preferred Specimen Instructions Reference Range Set-Up Schedule Specimen Requirement Stool Culture Enteric Pathogens 1099, STLC RMH Room Temperature Para-Pak (C&S) Preserved Stool, do not refrigerate or freeze Collect fresh stool in clean dry container. Using the collection spoon built into the lid place small scoopfuls of stool in the container. Mix with the spoon. Recap tightly and shake the tube vigorously until the contents are well mixed. For patients requiring the use of diapers, first line the diaper with clean plastic to prevent absorption. Then transfer 2 g or 2 ml of the stool specimen from the plastic lined diaper to the Para-Pak container. Do not submit the diaper itself. Cap securely. Stool will be cultured for the presence of Salmonella sp., Shigella sp., Campylobacter sp., Aeromonas, Plesiomonas and Escherichia coli H7:0157. Stool will not be checked for Yersinia enterocolitia or vibrios unless specifically requested. If other organisms are suspected, that fact must be noted under comments so appropriate techniques can be employed Conventional Culture Processed daily. Preliminary reports within 24 hours Fresh stool must be placed in the preservative within one hour of collection. Stool for WBC Gram stain 1486, STLWBC /RMH Specimens must tested immediately Fresh stool in Sterile Screw Cap Container, only one specimen accepted per day 5 grams 0.5 gram No WBC s seen Microscopic evaluation of gram stained smear WBC s rapidly degenerate in stool, evaluation of the stool after 2 hours post collection may yield false negative results. Page 9 of 9

Collection (Specimen Source Required on all tests) Sputum: >5 ml required. First morning specimen preferred.

Collection (Specimen Source Required on all tests) Sputum: >5 ml required. First morning specimen preferred. Type Acid Fast (Mycobacteria) Sputum: >5 ml required. First morning specimen preferred. For blood, sodium heparin tube preferred. Lithium heparin acceptable. Do not centrifuge.. delay. Swabs are not appropriate