BRIEF COMMUNICATIONS and CASE REPORTS
|
|
- Lizbeth Chapman
- 6 years ago
- Views:
Transcription
1 Vet Pathol 44: (2007) BRIEF COMMUNICATIONS and CASE REPORTS Metastatic Iridociliary Adenocarcinoma in a Labrador Retriever M. K. ZARFOSS AND R. R. DUBIELZIG Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI Abstract. An enucleated left eye from a 15-year-old female spayed Labrador Retriever was received by the Comparative Ocular Pathology Laboratory of Wisconsin (COPLOW) for histopathologic evaluation. Routine histologic preparation included staining with hematoxylin and eosin, and with alcian blue periodic acid Schiff (PAS). At necropsy 9 months later, all grossly abnormal tissues (ipsilateral orbit and lung) were submitted to the COPLOW for histopathologic evaluation. Histopathologic evaluation of the globe revealed extensive invasion of the uvea and sclera by a pleomorphic cell population that formed disorganized cords and exhibited PAS-positive basement membrane material. Necropsy revealed a morphologically similar tumor in the ipsilateral orbit and lung. On immunohistochemical examination, the intraocular tumor stained diffusely immunopositive for vimentin, S-100, and neuron-specific enolase and multifocally, sparsely immunopositive for cytokeratin AE1/AE3. The orbital and thoracic tumors stained positively for vimentin but negatively for cytokeratin AE1/AE3. There are few reports of canine metastatic iridociliary adenocarcinoma in the literature; this is the first with immunohistochemical analysis. Key words: Adenocarcinoma; dogs; eye; neoplasms; uvea. Iridociliary adenomas and adenocarcinomas arise from pigmented or nonpigmented iris or ciliary epithelium, and are the second most common primary intraocular tumor of dogs (second only to melanocytic tumors). 4,11 A review of canine iridociliary adenomas and adenocarcinomas by Peiffer 11 describes morphologic features and previous reports of these tumors. Although extraocular epithelial tumors and intraocular metastatic epithelial tumors are usually immunopositive for cytokeratin and immunonegative for vimentin, both normal iridociliary epithelium and iridociliary adenomas are immunohistochemically positive for vimentin and negative for cytokeratin AE1/ AE3. Iridociliary epithelial tumors are also characterized by S-100 and neuron-specific enolase (NSE) immunopositivity (Table 1). 5 The term iridociliary adenocarcinoma has been used for iridociliary epithelial tumors that are vimentin and cytokeratin AE1/AE3 positive, invade the sclera, and exhibit features of anaplasia. 5 A recent study found that expression of cytokeratins AE1 and AE3 was higher in scleroinvasive canine iridociliary adenocarcinomas compared with relatively well-differentiated iridociliary adenomas that more frequently expressed cytokeratin To date, no morphologic or immunohistochemical criteria have been associated with decreased survival in canine iridociliary tumors; however, there are few reports of unconfirmed 5 and confirmed metastasis. 6 Metastasis is likely a latestage phenomenon and is extremely unlikely in the absence of extensive scleral invasion. 10 Enucleation is both diagnostic and most reliably curative in benign cases; surgical excision or evisceration carries the risk of recurrence. 1,2 The purpose of this report is to describe the histologic features of a canine iridociliary adenocarcinoma that exhibited marked histologic features of malignancy, deeply invaded uveal, scleral, and orbital tissues, and metastasized to the lungs. An enucleated left globe from a 15-year-old female spayed Labrador Retriever was received by the Comparative Ocular Pathology Laboratory of Wisconsin (CO- PLOW) for histopathologic evaluation. After sectioning in the dorsoventral plane, the globe was embedded in paraffin, routinely sectioned (5-microns thick), and stained with hematoxylin and eosin (HE) and with alcian blue periodic acid Schiff (PAS) for evaluation. After euthanasia 9 months later, a full gross necropsy was performed by the referring veterinarian. All grossly abnormal tissues (left orbit and lung) and the grossly normal right eye were submitted to the COPLOW for histopathologic evaluation. These tissues were routinely stained with HE and alcian blue PAS as described above. Immunohistochemistry for vimentin, cytokeratin AE1/ AE3, S-100, and NSE was performed on all submitted tissues as previously described. Additional patient history was obtained directly from the referring veterinarian and the owner. Four immunohistochemistry, paraffin-embedded tissues were cut at 4 microns; mounted on charged slides; deparaffinized; and hydrated for 3 minutes each in 3 xylene baths, 3 absolute alcohol baths, and one 95% alcohol bath. Slides were then rinsed in tap water and treated for 5 minutes at room temperature with 3% H 2 O 2. Heat-induced epitope retrieval was used with a buffer at ph 6.0 at 97uC for 20 minutes in a vegetable steamer, then cooled outside the steamer for 20 minutes. The slides 672
2 Vet Pathol 44:5, 2007 Brief Communications and Case Reports 673 Table 1. Cytokeratin, vimentin, NSE, and S100 immunopositivity of various tissues and intraocular tumors.* Primary Antibodies Used in This Study{ Cytokeratin AE1/AE3 Vimentin NSE S-100 Clones AE1/AE3, No. M3515 (1 : 50) Clone V9, No. M0725 (1 : 200) No. M0873 DakoCytomation Polyclonal, No (1 : 2000) 1 Normal iridociliary 2 + epithelium 2 Iridociliary adenoma % of tumors + 3 Iridociliary + + adenocarcinoma 4 Intraocular tumor in this report 5 Orbital tumor in this report 6 Pulmonary tumor in this report * + 5 immunopositive; 2 5 immunonegative { Information listed in rows 1 3 is derived from the experience of the author (RRD) and the veterinary literature (from Ref. 5); immunohistochemistry in rows 4 6 was performed in this study. were then placed in phosphate-buffered saline solution (PBS, ph 6.0). On a Biogenex Optimax Plus auto stainer, slides were treated with BioGenex Power Block Universal Blocking Reagent (San Ramon, CA) for 10 minutes at room temperature to block nonspecific background staining. Slides were then incubated at room temperature for 30 minutes with the primary antibody (see Table 1), PBS washed, incubated with biotinylated secondary mouse/rabbit antibody for 20 minutes at room temperature, then PBS washed again. Tissues were incubated with streptavidin-peroxidase conjugate at room temperature for 20 minutes, then PBS washed. Slides were treated with a 0.05% 3-39-diaminobenzidine tetrahydrochloride solution with 1% H 2 O 2 for 5 minutes. Slides were removed from the stainer and placed in tap water. Slides were then counterstained with Harris hematoxylin for 2 minutes, dehydrated through progressive ethanol concentrations (50, 75, 95, and 100%) to xylene, and coverslipped (secondary antibody, streptavidin conjugate, and reagents from LSAB+Kit, Dako, Carpenteria, CA). Positive and negative controls accompanied all cases. This 15-year-old patient had a history of 4 prior tumors. Histopathology of these revealed an oral epulis, a subcutaneous lipoma, an incompletely excised subcutaneous peripheral nerve sheath tumor with low metastatic potential, and a perianal gland adenoma that was completely excised. The patient also had a severalyear history of unilateral cataract and a 6-month history of uveitis and secondary glaucoma in the left eye. Ultimately, an intravitreal gentamicin injection was performed in an attempt to control the glaucoma. Two months later, a circumferential thickening of the iris was noted clinically and precipitated enucleation. On gross examination, upon sectioning, much of the globe was internally lined and infiltrated by a tan mass (Figs. 1, 2). A pleomorphic amelanotic cell population formed disorganized cords and exhibited PAS-positive basement-membrane material, consistent with an epithelial tumor. Neoplastic cells extended from the iris base amid iridal necrosis to infiltrate full thickness through the limbus. Neoplastic cells exhibited a large nuclear to cytoplasmic ratio, prominent single-tomultiple nucleoli, marked anisocytosis and anaplasia (Fig. 3). The mitotic rate was 8 in 10 high-power fields. The ocular tumor expressed diffuse vimentin immunopositivity and few intensely positive areas of cytokeratin immunopositivity, making up between 5 and 10% of neoplastic tissue (Figs. 4 6) (Table 1). A collagen-rich neoplastic spindle-cell membrane filled the posterior chamber, cradled the lens, lined the inner aspect of the choroid, and infiltrated the sclera around the optic nerve. There was extensive lens capsular wrinkling in the absence of evidence for lens capsule rupture, consistent with hypermature cataract. Nine months after enucleation, the patient experienced lethargy and nasal discharge ipsilateral to the enucleation site. Thoracic radiographs revealed multiple pulmonary nodules. The patient was humanely euthanatized. At necropsy, all organs were within normal limits, except for the left orbit and lungs. The right eye was histologically normal. Orbital tissue was heavily infiltrated by an anaplastic neoplasm composed primarily of spindle-shaped cells. Orbital neoplastic cells were vimentin, S-100, and NSE positive and cytokeratin negative (Table 1). Multiple pulmonary nodules were within and surrounding blood vessels, consistent with a metastatic neoplasm (Fig. 7). These neoplastic cells were anaplastic and morphologically similar to the cells within the orbit (also vimentin, S100, and NSE positive and cytokeratin negative). The distinction between iridociliary adenomas and adenocarcinomas is not clear within the human and veterinary literature. In humans, iridociliary epithelial
3 674 Brief Communications and Case Reports Vet Pathol 44:5, 2007 Fig. 1. Eye; canine. Gross photograph. The globe was internally lined and infiltrated by a tan mass. Formalin fixation. Ruler 5 1 cm. Fig. 2. Eye; canine. Subgross photograph. HE. Fig. 3. Uvea; canine. Anaplastic, pleomorphic neoplastic cells form invasive clusters, extending through the limbal sclera. HE. Bar 5 50 microns. Fig. 4. Uvea; canine. Vimentin, streptavidin-biotin indirect immunoperoxidase immunohistochemistry. Fig. 5. Uvea; canine. Same magnification as Fig. 4. Cytokeratin AE1/AE3, streptavidin-biotin indirect immunoperoxidase immunohistochemistry. Fig. 6. Uvea; canine. Neoplastic tissue extends from the pigmented iris (right) to the lens capsule (left). This image is at lower magnification than Figs. 4 and 5, showing diffuse immunoreactivity of the tumor. The star is in the same anatomic location in Figs Vimentin, streptavidin-biotin indirect immunoperoxidase immunohistochemistry. Bar microns. tumors are much less common than in dogs, and histologic features of malignancy vary widely. In 1 study, human iridociliary adenocarcinomas with orbital extension metastasized to the parotid gland and distant bones. 10 A subset of human iridociliary tumors is associated with a history of trauma (including surgical trauma), intraocular inflammation, or phthisis bulbi. 3,7 The only known incident of trauma in this case was a gentamicin intravitreal injection. The gentamicin-induced intraocular inflammation would have caused further compromise of the blood-ocular barrier and could have affected tumor morphology. In this case, the intraocular tumor showed sparse, strong, cytokeratinimmunopositive areas that were not maintained within the morphologically similar orbital or pulmonary tumors. Extraocular epithelial tumors are generally immunopositive for cytokeratin and immunonegative for vimentin. However, rare epithelial tumors are vimentin
4 Vet Pathol 44:5, 2007 Brief Communications and Case Reports 675 Fig. 7. Lung; canine. Larger image is lung, inset at higher magnification. Multiple pulmonary nodules are present within and surrounding blood vessels. Neoplastic cells are morphologically similar to the intraocular tumor. HE. Bar (larger image) microns, Bar (inset) 5 60 microns. positive, suggesting a potentially more aggressive, mesechymal-cell variant. 8 Loss of cytokeratin reactivity and gain of vimentin reactivity was reported in metastatic epithelial tumors as part of a malignant transformation. 12 The loss of cytokeratin immunoreactivity in the extraocular tumors in this case was most likely because of tumor cell anaplasia. Acknowledgements The authors would like to thank the owners of this patient, Dr. Kelly Carver and Dr. Jennifer Chilton, for allowing the necropsy, performing the necropsy, and perusing the manuscript. Immunohistochemistry was accomplished with the expertise of Lona K. Barsness, Anne Slattery, and Sandra Cheasty, HT (ASCP). References 1 Bellhorn RW: Ciliary body adenocarcinoma in the dog. J Am Vet Med Assoc 159: , Beltran W, Chahory S, Clerc B: Complication d implantation d une prothese intra-oculaire par proliferation d un adenocarcinome iridociliaire chez un chien. [Complication of implantation of intraocular prosthesis by proliferation of an iridociliary adenocarcinoma in a dog] Le Point Veterinaire 31:55 59, Dryja TP, Zakov ZN, Albert DM: Adenocarcinoma arising from the epithelium of the iris and ciliary body. Int Ophthalmol Clin 20: , Dubielzig RR: Tumors of the eye. In: Tumors in Domestic Animals, ed. Meuten DJ, pp Iowa State Press, Ames, IA, USA, Dubielzig RR, Steinberg H, Garvin H, Deehr AJ, Fischer B: Iridociliary epithelial tumors in 100 dogs and 17 cats: a morphological study. Vet Ophthalmol 1: , Glickstein JM, Allen HL: Malignant ciliary body adenocarcinoma in a dog. J Am Vet Med Assoc 165: , Grossniklaus HE, Zimmerman LE, Kachmer ML: Pleomorphic adenocarcinoma of the ciliary body. Immunohistochemical and electron microscopic features. Ophthalmology 97: , 1990
5 676 Brief Communications and Case Reports Vet Pathol 44:5, Hammar SP: Lung and pleural neoplasms. In: Diagnostic Immunohistochemistry, ed. Dabbs DJ, pp Churchill Livingston, New York, NY, USA, Klosterman E, Colitz CM, Chandler HL, Kusewitt DF, Saville WJ, Dubielzig RR: Immunohistochemical properties of ocular adenomas, adenocarcinomas and medulloepitheliomas. Vet Ophthalmol 9: , Laver NM, Hidayat AA, Croxatto JO: Pleomorphic adenocarcinomas of the ciliary epithelium. Immunohistochemical and ultrastructural features of 12 cases. Ophthalmology 106: , Peiffer RL Jr: Ciliary body tumours in the dog and cat; a report of thirteen cases. J Small Anim Pract 24: , Thiery JP: Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer 2: , 2002 Request reprints from Richard R. Dubielzig, Department of Pathobiological Sciences, University of Wisconsin- Madison 2015 Linden Dr. Madison, WI (USA). dubielzr@svm.vetmed.wisc.edu.
Proceedings of the 36th World Small Animal Veterinary Congress WSAVA
www.ivis.org Proceedings of the 36th World Small Animal Veterinary Congress WSAVA Oct. 14-17, 2011 Jeju, Korea Next Congress: Reprinted in IVIS with the permission of WSAVA http://www.ivis.org 14(Fri)
More informationOcular Neoplasia What s Common? What s New? Richard R Dubielzig
Ocular Neoplasia What s Common? What s New? Richard R Dubielzig Orbit 288 6% Tumors of the globe make up 3225 out of 6110 total neoplasms = 53%. Tumors of the conjunctiva make up 1192 out of 6110 total
More informationOcular Neoplasia CL Davis 9/08. Richard R Dubielzig
Ocular Neoplasia CL Davis 9/08 Richard R Dubielzig 2135/5722 Canine Melanocytic Tumors Outside the Globe: 264 Conjunctival: 159 Eye Lid: 72 Skin: 33 Affecting the Globe: 1871 Anterior Uveal Melanocytoma:
More informationGeneral physical examination was unremarkable. No history of previous ocular problems.
AQUEOUS HUMOUR FROM A DOG H Ferreira 1, E Scurrell 2, J Bass 3, K Salmon 4 1: Axiom Veterinary Laboratories, Newton Abbot, UK 2: CYTOPATH Limited, Ledbury, Herefordshire, UK 3: Finn Pathologists, Weybread,
More informationAsadi-Amoli et al Adenocarcinoma of RPE Iranian Journal of Ophthalmology - Volume 19, Number 4, 2007
Adenocarcinoma of Retinal Pigment Epithelium Clinically Diagnosed as Malignant Melanoma; A Case Report with Unsystematic Review of Literature Fahimeh Asadi-Amoli, MD, 1 Hedyeh Moradi, MD 2 Mohammad-Taher
More informationFELINE DIFFUSE IRIDAL MELANOMA
Vet Times The website for the veterinary profession https://www.vettimes.co.uk FELINE DIFFUSE IRIDAL MELANOMA Author : JAMES OLIVER Categories : Vets Date : June 3, 2013 JAMES OLIVER looks at several clinical
More informationAdenocarcinorna of the Ciliary Body A Report of 2 Cases in Dogs
Path. vet. 5: 122-126 (1968) From the Ophthalmic Pathology Laboratory, Department of Ophthalmology, New York University School of Medicine, New York Adenocarcinorna of the Ciliary Body A Report of 2 Cases
More informationFORELIMB SWEAT GLAND ADENOCARCINOMA IN A CAT
I: 2047-2051 ISSN: 2277 4998 FORELIMB SWEAT GLAND ADENOCARCINOMA IN A CAT ABEDI G 1, HESARAKI S 2, ASGHARI A 1* 1: Department of Clinical Science, Science and Research branch, Islamic Azad University,
More informationIntraocular sarcomas in two rabbits. <!?show "fnote_aff1"$^!"content-markup(./author-grp[1]/aff./author-grp[1]/dept-list)>
Case Reports 547 J Vet Diagn Invest 21:547 551 (2009) Intraocular sarcomas in two rabbits Leslie McPherson, Shelley J. Newman, 1 Nancy McLean, Stephanie McCain, Ramesh Vemulapalli, Stephen Kania, Richard
More informationGross Ocular Pathology
Gross Ocular Pathology 06rd0850 Feline Metastatic sarcoma 06rd0878 Feline Cryptococcosis 06rd0880 Canine Malignant Melanoma 06rd0929 Feline Conjunctivalization of the epithelium 06rd0931 Name the Species
More informationUveal Melanoma. Protocol applies to malignant melanoma of the uvea.
Uveal Melanoma Protocol applies to malignant melanoma of the uvea. Protocol revision date: January 2005 Based on AJCC/UICC TNM, 6 th edition Procedures Cytology (No Accompanying Checklist) Biopsy (No Accompanying
More informationMesectodermal suprauveal iridociliary leiomyoma: Transscleral excision without postoperative iris defect
4 Chapter Mesectodermal suprauveal iridociliary leiomyoma: Transscleral excision without postoperative iris defect Lubna Razzaq 1, Ekaterina A Semenova 2, Marina Marinkovic 1, Rob JW de Keizer 1, Sjoerd
More informationRetina Center of Oklahoma Sam S. Dahr, M.D. Adult Intraocular Tumors
Adult Intraocular Tumors Sam S. Dahr, M.D. Retina Center of Oklahoma www.retinacenteroklahoma.com www.rcoklahoma.com Table of Contents Posterior uveal malignant melanoma Uveal metastasis Uveal melanoma
More informationCLINICAL PRESENTATION, DIAGNOSTIC AND THERAPEUTIC APPROACH OF OCULAR MELANOSIS IN A GOLDEN RETRIEVER- CASE STUDY
Scientific Works. Series C. Veterinary Medicine. Vol. LIX (3) ISSN 2065-1295, ISSN Online 2067-3663, ISSN-L 2065-1295 CLINICAL PRESENTATION, DIAGNOSTIC AND THERAPEUTIC APPROACH OF OCULAR MELANOSIS IN A
More informationDisorders of Cell Growth & Neoplasia. Histopathology Lab
Disorders of Cell Growth & Neoplasia Histopathology Lab Paul Hanna April 2010 Case #84 Clinical History: 5 yr-old, West Highland White terrier. skin mass from axillary region. has been present for the
More informationDiffuse infiltrating retinoblastoma
Brit. 1. Ophthal. (I 971) 55, 6oo Diffuse infiltrating retinoblastoma GWYN MORGAN Department of Pathology, Institute of Ophthalmology, University of London The term "diffuse infiltrating retinoblastoma"
More informationUNUSUAL OCULAR HODGKINʼS-LIKE LYMPHOMA IN A DOG
Bulgarian Journal of Veterinary Medicine, 2019 ONLINE FIRST ISSN 1311-1477; DOI: 10.15547/bjvm.2241 Case report UNUSUL OCULR HODGKINʼS-LIKE LYMPHOM IN DOG Summary M. KHORDDMEHR 1, S. JROLMSJED 2, J. SHRFI-HELN
More informationAmong the benign intraepithelial melanocytic proliferations, Inflamed Conjunctival Nevi. Histopathological Criteria. Resident Short Reviews
Resident Short Reviews Inflamed conjunctival nevi (ICN) may suggest malignancy because of their rapid growth and atypical histology. The objective of this study was to characterize the diagnostic features
More informationA Canine Case of Complex Carcinoma of the Mammary Gland with Metastasis to the Axillary Lymph Node
A Canine Case of Complex Carcinoma of the Mammary Gland with Metastasis to the Axillary Lymph Node Kenjiro Hashimoto 1), Atsushi Kawabata 1), Tamio Ohmuro 2), Kinji Shirota 1, 3) * 1) Research Institute
More informationThe Pathology and Pathogenesis of Acute Glaucoma in Dogs. Richard R Dubielzig
The Pathology and Pathogenesis of Acute Glaucoma in Dogs Richard R Dubielzig Overview of Glaucoma Intraocular Pressure too High to Support a Healthy Optic Nerve Terminology used in the classification of
More informationNeoplasia 2018 Lecture 2. Dr Heyam Awad MD, FRCPath
Neoplasia 2018 Lecture 2 Dr Heyam Awad MD, FRCPath ILOS 1. List the differences between benign and malignant tumors. 2. Recognize the histological features of malignancy. 3. Define dysplasia and understand
More informationNeoplasms of the Canine, Feline and Lemur Liver:
Neoplasms of the Canine, Feline and Lemur Liver: Classification and Prognosis Annual Seminar of the French Society of Veterinary Pathology John M. Cullen VMD PhD DACVP North Carolina State University Primary
More informationAtypical Palisaded Myofibroblastoma of Lymph Node: Report of a rare case.
ISPUB.COM The Internet Journal of Pathology Volume 10 Number 1 Atypical Palisaded Myofibroblastoma of Lymph Node: Report of a rare case. V Kinnera, R Nandyala, M Yootla, K Mandyam Citation V Kinnera, R
More informationCase Report A Rare Cutaneous Adnexal Tumor: Malignant Proliferating Trichilemmal Tumor
Case Reports in Medicine Volume 2015, Article ID 742920, 4 pages http://dx.doi.org/10.1155/2015/742920 Case Report A Rare Cutaneous Adnexal Tumor: Malignant Proliferating Trichilemmal Tumor Omer Alici,
More informationA study of iris melanoma in Northern Ireland
British Journal of Ophthalmology, 1989, 73, 591-595 A study of iris melanoma in Northern Ireland J N McGALLIARD AND P B JOHNSTON From the Department of Ophthalmology, Royal Victoria Hospital, Grosvenor
More informationOcular melanocytic neoplasia in dogs
CE Article #1 Michael Finn, DVM, MS* Sheryl Krohne, DVM, MS, DACVO Jean Stiles, DVM, MS, DACVO Purdue University ABSTRACT: Tumors of melanocytic origin in ocular tissues are relatively uncommon in dogs
More informationAround The Globe in 60 Minutes
Around The Globe in 60 Minutes Around the GLOBE in Sixty Minutes Basic Ocular Anatomy, Examination, and Diagnostic Techniques Introduction Focusing on canine and feline ocular anatomy and basic examination
More informationLIST OF ORGANS FOR HISTOPATHOLOGICAL ANALYSIS:!! Neural!!!!!!Respiratory:! Brain : Cerebrum,!!! Lungs and trachea! Olfactory, Cerebellum!!!!Other:!
LIST OF ORGANS FOR HISTOPATHOLOGICAL ANALYSIS:!! Neural!!!!!!Respiratory:! Brain : Cerebrum,!!! Lungs and trachea! Olfactory, Cerebellum!!!!Other:! Spinal cord and peripheral nerves! Eyes, Inner ear, nasal
More informationPathology of the lens
Pathology of the lens Carol Naranjo, LV, DACVP, DECVP, PhD IDEXX Laboratories Embryonal development Gelatt s Veterinary Ophthalmology, 5th Ed. Normal histology Lens capsule Anterior > posterior Lens cortex
More informationC.L. Davis Foundation Descriptive Veterinary Pathology Course
C.L. Davis Foundation 2015 Descriptive Veterinary Pathology Course IHC Resources IHC Identification Targets Antibodies Antibodies 1 Antibodies Specimens Antigen Retrieval Unmasks antigen epitopes Methods
More information2008 Gross Ocular Pathology. Gross Pathology 2
2008 Gross Ocular Pathology Gross Pathology 2 08rd1281 Feline T-Cell Lymphoma 08rd1300 Canine Iridociliary Adenoma Foam Cell Variant 08rd1331 Feline Feline Iridociliary Adenoma 08rd1340 Canine Retinal
More informationCiliary Body Metastasis Masquerading as Scleritis. Brian J. Lee, MD 1. Careen Y. Lowder, MD, PhD 1. Charles Biscotti, MD 2. Lynn Schoenfield, MD 2
Ciliary Body Metastasis Masquerading as Scleritis Brian J. Lee, MD 1 Careen Y. Lowder, MD, PhD 1 Charles Biscotti, MD 2 Lynn Schoenfield, MD 2 Arun D. Singh, MD 1 Cole Eye Institute 1 and Department of
More informationCanine Cutaneous Melanoma
Canine Cutaneous Melanoma By Elizabeth Downing Clinical Advisor: Dr. Angharad Waite, VMD Basic Science Advisor: Dr. Cheryl Balkman, DVM, DACVIM Senior Seminar Paper Cornell University College of Veterinary
More informationCell Culture. The human thyroid follicular carcinoma cell lines FTC-238, FTC-236 and FTC-
Supplemental material and methods Reagents. Hydralazine was purchased from Sigma-Aldrich. Cell Culture. The human thyroid follicular carcinoma cell lines FTC-238, FTC-236 and FTC- 133, human thyroid medullary
More informationSpinal Cord Compression caused by Metastatic Epithelial Myoepithelial Carcinoma of the Parotid Gland
Spinal Cord Compression caused by Metastatic Epithelial Myoepithelial Carcinoma of the Parotid Gland Pages with reference to book, From 249 To 250 Irshad N. Soomro,Akber S. Hussainy,Rashida Ahmed,Sheema
More informationAppendix 1. A. Procedure for preparing histopathology slides. The liver removed and stored immediately in buffered formalin 10 % for
Appendix 1 A. Procedure for preparing histopathology slides. The liver removed and stored immediately in buffered formalin 10 % for histopathological examination. The tissue fixed for at least 48 hours
More informationComparative Ocular Pathology Rounds
Comparative Ocular Pathology Rounds 2007 2 07RD0943 Bovine Anterior Segment Dysgenesis 07RD1072 Feline Feline post-traumatic sarcoma spindle cell variant 07rd1124 Canine Stick in the Eye 07rd1132 Feline
More informationSelected Diseases of the Cornea Dick Dubielzig July 20 th, 2009
Selected Diseases of the Cornea Dick Dubielzig July 20 th, 2009 Canine Collagenolytic Keratitis Canine Collagenolytic Keratitis 270 cases (1.5%) Usually associated with suppurative keratitis Collagenolytic
More informationA case of giant cell tumour of soft parts in a horse Francesco Cian 1, Sarah Whiteoak 2, Jennifer Stewart 1
A case of giant cell tumour of soft parts in a horse Francesco Cian 1, Sarah Whiteoak 2, Jennifer Stewart 1 1 Animal Health Trust, Newmarket, UK 2 608 Equine and Farm Vets, Rowington, UK Signalment: Horse,
More informationDiplomate of the American Board of Pathology in Anatomic and Clinical Pathology
A 33-year-old male with a left lower leg mass. Contributed by Shaoxiong Chen, MD, PhD Assistant Professor Indiana University School of Medicine/ IU Health Partners Department of Pathology and Laboratory
More informationScience & Technologies A RETROSPECTIVE STUDY OF CANINE SKIN ROUND CELL TUMOURS
A RETROSPECTIVE STUDY OF CANINE SKIN ROUND CELL TUMOURS Radostin Simeonov Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria, e-mail:
More informationA 25 year old female with a palpable mass in the right lower quadrant of her abdomen
May 2016 A 25 year old female with a palpable mass in the right lower quadrant of her abdomen Contributed by: Paul Ndekwe, MD, Resident Physician, Indiana University School of Department of Pathology and
More informationEnterprise Interest Nothing to declare
Enterprise Interest Nothing to declare Diagnoses one would not like to miss in soft tissue pathology early in your career Marta Sbaraglia, MD Department of Pathology Hospital of Treviso University of Padua
More informationPapillary Lesions of the Breast A Practical Approach to Diagnosis. (Arch Pathol Lab Med. 2016;140: ; doi: /arpa.
Papillary Lesions of the Breast A Practical Approach to Diagnosis (Arch Pathol Lab Med. 2016;140:1052 1059; doi: 10.5858/arpa.2016-0219-RA) Papillary lesions of the breast Span the spectrum of benign,
More informationDiagnostic Immunohistochemistry of Canine Round Cell Tumors
Vet. Pathol. 24495-499 (1987) Diagnostic Immunohistochemistry of Canine Round Cell Tumors G. E. SANDUSKY, W. W. CAIUTON, AND K. A. WIGHTMAN Lilly Research Laboratories, A Division of Eli Lilly and Company,
More informationBSD 2015 Case 19. Female 21. Nodule on forehead. The best diagnosis is:
BSD 2015 Case 19 Female 21. Nodule on forehead. The best diagnosis is: A. mixed tumour of skin B. porocarcinoma C. nodular hidradenoma D. metastatic adenocarcinoma BSD 2015 Case 19 Female 21 Nodule on
More informationPediatric Ocular Sonography
Pediatric Ocular Sonography Cicero J Torres A Silva, MD Associate Professor of Radiology 2016 SPR Pediatric Ultrasound Course Yale University School of Medicine None Disclosures Objectives of Presentation
More informationCoagulative necrosis in a malignant melanoma of the choroid at the macula with extensive subretinal hemorrhage
Coagulative necrosis in a malignant melanoma of the choroid at the macula with extensive subretinal hemorrhage Robert D. Yee, Robert Y. Foos, and Bradley R. Straatsma The authors present a case report
More informationSolitary Fibrous Tumor of the Kidney with Massive Retroperitoneal Recurrence. A Case Presentation
246) Prague Medical Report / Vol. 113 (2012) No. 3, p. 246 250 Solitary Fibrous Tumor of the Kidney with Massive Retroperitoneal Recurrence. A Case Presentation Sfoungaristos S., Papatheodorou M., Kavouras
More informationHistopathology: skin pathology
Histopathology: skin pathology These presentations are to help you identify, and to test yourself on identifying, basic histopathological features. They do not contain the additional factual information
More informationKidney Case 1 SURGICAL PATHOLOGY REPORT
Kidney Case 1 Surgical Pathology Report February 9, 2007 Clinical History: This 45 year old woman was found to have a left renal mass. CT urography with reconstruction revealed a 2 cm medial mass which
More informationNeoplasia part I. Dr. Mohsen Dashti. Clinical Medicine & Pathology nd Lecture
Neoplasia part I By Dr. Mohsen Dashti Clinical Medicine & Pathology 316 2 nd Lecture Lecture outline Review of structure & function. Basic definitions. Classification of neoplasms. Morphologic features.
More informationHistopathological Study of Lacrimal Gland Tumors
ORIGINAL ARTICLE Pratikkumar B. Desai 1, Ami Shah 2 1 4 th Year Resident, Pathology Department, B.J.Medical College, Civil Hospital, Ahmedabad 2 Associate Professor, M. J. Institute of Ophthalmology, Civil
More informationPulmonary Salivary Gland Type Tumors With Features of Malignant Mixed Tumor (Carcinoma Ex Pleomorphic Adenoma) A Clinicopathologic Study of Five Cases
Anatomic Pathology / Pulmonary Salivary Gland Type Tumors Pulmonary Salivary Gland Type Tumors With Features of Malignant Mixed Tumor (Carcinoma Ex Pleomorphic Adenoma) A Clinicopathologic Study of Five
More information3/27/2017. Disclosure of Relevant Financial Relationships
Ophthalmic Pathology Evening Specialty Conference USCAP 2017 5 th March, 2017 Mukul K. Divatia, MD Assistant Professor Department of Pathology & Genomic Medicine Weill Cornell Medical College Houston Methodist
More informationA neoplasm is defined as "an abnormal tissue proliferation, which exceeds that of adjacent normal tissue. This proliferation continues even after
NEOPLASIA Neoplasia is a very important topic in pathology because neoplasms are both common and serious diseases. A neoplasm literally means a new growth, and this term is used interchangeably with a
More informationCLINICAL PEARLS IN OCULAR ONCOLOGY
CLINICAL PEARLS IN OCULAR ONCOLOGY IRIS NEVUS - Two kinds circumscribed and diffuse - Photodocumentation important to monitor growth - Risk Factors for iris nevus growth to melanoma (ABCDEF) A Age (young),
More informationSynonyms. Nephrogenic metaplasia Mesonephric adenoma
Nephrogenic Adenoma Synonyms Nephrogenic metaplasia Mesonephric adenoma Definition Benign epithelial lesion of urinary tract with tubular, glandular, papillary growth pattern Most frequently in the urinary
More informationWT1, Estrogen Receptor, and Progesterone Receptor as Markers for Breast or Ovarian Primary Sites in Metastatic Adenocarcinoma to Body Fluids
Anatomic Pathology / WT1, ESTROGEN RECEPTOR, AND PROGESTERONE RECEPTOR IN CYTOLOGY OF BODY FLUIDS WT1, Estrogen Receptor, and Progesterone Receptor as Markers for Breast or Ovarian Primary Sites in Metastatic
More informationType IV collagen and laminin staining patterns in benign
J Clin Pathol 1989;42:1173-1177 Type IV collagen and laminin staining patterns in benign and malignant cutaneous lesions RONA M MacKIE, D B CLELLAND, CHRISTINE J SKERROW From the Department ofdermatology,
More informationNeoplasia literally means "new growth.
NEOPLASIA Neoplasia literally means "new growth. A neoplasm, defined as "an abnormal mass of tissue the growth of which exceeds and is uncoordinated with that of the normal tissues and persists in the
More informationOcular Pathology. I. Congenital and/or developmental. A. Trisomy 21. Hypertelorism (widely spaced eyes) Keratoconus (cone shaped cornea)
I. Congenital and/or developmental Robbins Pathologic Basis of Disease, 6 th Ed. A. Trisomy 21 Hypertelorism (widely spaced eyes) Keratoconus (cone shaped cornea) Focal hypoplasia of iris Cataracts frequently
More informationOPHTHALMOLOGY AND ULTRASOUND
Vet Times The website for the veterinary profession https://www.vettimes.co.uk OPHTHALMOLOGY AND ULTRASOUND Author : JAMES OLIVER Categories : Vets Date : April 28, 2008 JAMES OLIVER discusses why ultrasound
More informationDesmoplastic Melanoma R/O BCC. Clinical Information. 74 y.o. man with lesion on left side of neck r/o BCC
R/O BCC Sabine Kohler, M.D. Professor of Pathology and Dermatology Dermatopathology Service Stanford University School of Medicine Clinical Information 74 y.o. man with lesion on left side of neck r/o
More informationCase # nd Annual SEVPAC May 17, Kathy-Anne Clarke
Case # 10 42 nd Annual SEVPAC May 17, 2014 Kathy-Anne Clarke Google images Babu Babu is 10 year old spayed female French Bulldog Chronic weight loss over 4 months Febrile and lethargic at the referring
More informationImmunohistochemical Evaluation of Necrotic Malignant Melanomas
Anatomic Pathology / EVALUATION OF NECROTIC MALIGNANT MELANOMAS Immunohistochemical Evaluation of Necrotic Malignant Melanomas Daisuke Nonaka, MD, Jordan Laser, MD, Rachel Tucker, HTL(ASCP), and Jonathan
More informationNEOPLASIA-I CANCER. Nam Deuk Kim, Ph.D.
NEOPLASIA-I CANCER Nam Deuk Kim, Ph.D. 1 2 Tumor in the hieroglyphics of the Edwin Smith papyrus (1,600 B.C., Breasted s translation 1930) 3 War on Cancer (National Cancer Act, 1971) 4 Cancer Acts in Korea
More informationMaligna Melanoma and Atypical Fibroxanthoma: An Unusual Collision Tumour G Türkcü 1, A Keleş 1, U Alabalık 1, D Uçmak 2, H Büyükbayram 1 ABSTRACT
Maligna Melanoma and Atypical Fibroxanthoma: An Unusual Collision Tumour G Türkcü 1, A Keleş 1, U Alabalık 1, D Uçmak 2, H Büyükbayram 1 ABSTRACT Two different neoplasia in the same biopsy material called
More informationRetinoblastoma. Protocol applies to retinoblastoma only.
Retinoblastoma Protocol applies to retinoblastoma only. Protocol revision date: January 2005 Based on AJCC/UICC TNM, 6 th edition Procedures Cytology (No Accompanying Checklist) Biopsy (No Accompanying
More informationDispelling Rumors about Tumors. Case
Dispelling Rumors about Tumors Jesse L. Berry, MD Arizona Ophthalmology Society 2017 Associate Director, Ocular Oncology Service Associate Program Director USC/CHLA, Keck School of Medicine Case 65 year
More informationProceedings of the Society for Theriogenology 2012 Annual Conference
www.ivis.org Proceedings of the Society for Theriogenology 2012 Annual Conference Aug. 20-25, 2012 Baltimore, MD, USA www.therio.org/ Next Meeting: Aug. 7-10, 2013 Louisville, KY, USA Reprinted in the
More informationXUE HUI Department of Histology& Embryology, Basic Medicine College of Jilin University
SENSE ORGAN XUE HUI Department of Histology& Embryology, Basic Medicine College of Jilin University EYE fibrous globe lens photosensitive cells a system of cells and nerves concentric layers the sclera
More informationUniversity Journal of Pre and Para Clinical Sciences
ISSN 2455 2879 Volume 2 Issue 1 2016 Metaplastic carcinoma breast a rare case report Abstract : Metaplastic carcinoma of the breast is a rare malignancy with two distinct cell lines described as a breast
More informationnumber Done by Corrected by Doctor Maha Shomaf
number 16 Done by Waseem Abo-Obeida Corrected by Zeina Assaf Doctor Maha Shomaf MALIGNANT NEOPLASMS The four fundamental features by which benign and malignant tumors can be distinguished are: 1- differentiation
More informationPrinciples of Surgical Oncology. Winnie Achilles Tierklinik Hollabrunn Lastenstrasse Hollabrunn
Principles of Surgical Oncology Winnie Achilles Tierklinik Hollabrunn Lastenstrasse 2 2020 Hollabrunn boexi@gmx.de The first surgery provides the best chance for a cure in an animal with a tumor Clinical
More informationOcular disorders known or presumed to be inherited (published) Iris and ciliary body cysts ; 2-6 y.o. ; possible relationship with glaucoma
Golden Retriever Ocular disorders known or presumed to be inherited (published) Diagnosis Description and comments specific to the breed Inheritance Gene/ marker test References A Uveal cysts Iris and
More informationGastric Carcinoma with Lymphoid Stroma: Association with Epstein Virus Genome demonstrated by PCR
Gastric Carcinoma with Lymphoid Stroma: Association with Epstein Virus Genome demonstrated by PCR Pages with reference to book, From 305 To 307 Irshad N. Soomro,Samina Noorali,Syed Abdul Aziz,Suhail Muzaffar,Shahid
More informationMammary Nodular Hyperplasia in Intact R hesus Monkeys
Vet. Path. 10: 130-134 (1973) Mammary Nodular Hyperplasia in Intact R hesus Monkeys L. W NELSON and L. D. SHOTT Department of Pathology and Toxicology, Mead Johnson Research Center, Evansville, Ind., and
More informationSynchronous Multicentric Osteosarcoma in the Canine Axial Skeleton: a Report of Two Cases
173 Case Report Synchronous Multicentric Osteosarcoma in the Canine Axial Skeleton: a Report of Two Cases Matheus V. L. Moreira 1, Juneo F. Silva 1, Jankerle N. Boeloni 1, Natalia M. Ocarino 1, Rogéria
More informationTitleSarcomatoid carcinoma of the bladde.
TitleSarcomatoid carcinoma of the bladde Author(s) Takashi, Munehisa; Sakata, Takao; N Tatsuya; Miyake, Koji Citation 泌尿器科紀要 (1992), 38(1): 67-70 Issue Date 1992-01 URL http://hdl.handle.net/2433/117446
More informationAll authors abide by the Association for Medical Ethics (AME) ethical rules of disclosure.
Longo F, Musumeci G, Parenti R, Vecchio G, Magro G. Atypical cell leiomyoma of the uterus with amianthoid-like fibers: A case report. OA Case Reports 2013 Nov 15;2(14):137. Licensee OA Publishing London
More informationIris-Ciliary Body Melanoma: 57-year-old female with iris lesion
Iris-Ciliary Body Melanoma: 57-year-old female with iris lesion Gina M. Rogers, MD, Nasreen A. Syed, MD, Wallace L. M. Alward, MD, Juan Fernandez de Castro, MD, Lauren Jensen, BS Chief Complaint: Spot
More informationPLEOMORPHIC ADENOMA ( BENIGN MIXED TUMOR )
( BENIGN MIXED TUMOR ) Grossly, the tumor is freely movable, solid, sometimes lobulated and occasionally cystic. If recurrent, multinodular masses are common. Histologically, within a fibrous capsule,
More informationComplicated Cataract to Intraocular Tumors, Beware of the unexpected
Complicated Cataract to Intraocular Tumors, Beware of the unexpected Ihab Saad Othman, MD, FRCS Professor of Ophthalmology Cairo University In this part of the world: We Master Phakoemulsification 1 Intraoperative/Second
More informationRuthenium-106 plaque brachytherapy in the primary management of ocular medulloepithelioma
Poon, DS; Reich, E; Smith, VM; Kingston, J; Reddy, MA; Hungerford, JL; Sagoo, MS; (2015) Ruthenium-106 Plaque Brachytherapy in the Primary Management of Ocular Medulloepithelioma. Ophthalmology, 122 (9)
More informationHistology of the Eye
Histology of the Eye Objectives By the end of this lecture, the student should be able to describe: The general structure of the eye. The microscopic structure of:»cornea.»retina. EYE BULB Three coats
More informationProceedings of the 36th World Small Animal Veterinary Congress WSAVA
www.ivis.org Proceedings of the 36th World Small Animal Veterinary Congress WSAVA Oct. 14-17, 2011 Jeju, Korea Next Congress: Reprinted in IVIS with the permission of WSAVA http://www.ivis.org 14(Fri)
More informationCASE REPORT Benign epithelioid peripheral nerve sheath tumour resembling schwannoma
Malaysian J Pathol 2014; 36(3) : 217 221 CASE REPORT Benign epithelioid peripheral nerve sheath tumour resembling schwannoma Thejasvi KRISHNAMURTHY MD and SR NIVEDITHA MD, DNB Department of Pathology,
More informationTable of Contents 1 Orbit 3 2 Eyelids 7
Table of Contents Preface, x List of abbreviations xi Glossary xii Section I Atlas 1 1 Orbit 3 Clinical signs associated with orbital neoplasia 3 Clinical signs associated with orbital cellulitis 3 Enophthalmos
More information(A) PCR primers (arrows) designed to distinguish wild type (P1+P2), targeted (P1+P2) and excised (P1+P3)14-
1 Supplemental Figure Legends Figure S1. Mammary tumors of ErbB2 KI mice with 14-3-3σ ablation have elevated ErbB2 transcript levels and cell proliferation (A) PCR primers (arrows) designed to distinguish
More informationG3.02 The malignant potential of the neoplasm should be recorded. CG3.02a
G3.02 The malignant potential of the neoplasm should be recorded. CG3.02a Conventional adrenocortical neoplasm. Each of the below parameters is scored 0 when absent and 1 when present. 3 or more of these
More informationDisclosures. Parathyroid Pathology. Objectives. The normal parathyroid 11/10/2012
Disclosures Parathyroid Pathology I have nothing to disclose Annemieke van Zante MD/PhD Assistant Professor of Clinical Pathology Associate Chief of Cytopathology Objectives 1. Review the pathologic features
More informationMapping Basal Cell and Squamous Carcinoma By 10 Min CK5 Direct Immunohistochemistry on Frozen Section Skin Tissues during Mohs Micrographic Surgery
Mapping Basal Cell and Squamous Carcinoma By 10 Min CK5 Direct Immunohistochemistry on Frozen Section Skin Tissues during Mohs Micrographic Surgery Robert Glinert, MD and Song Q. Zhao, MD, Ph.D., MPH Department
More informationGlaucoma. Glaucoma. Glaucoma. Trevor Arnold, MS, DVM, DACVO
Glaucoma Trevor Arnold, MS, DVM, DACVO Glaucoma Physiology of Aqueous Humor Produced in the ciliary body Flows out the iridocorneal angle and ciliary cleft High intraocular pressures are caused by a decreased
More informationThis protocol is intended to assist pathologists in providing
Protocol for the Examination of Specimens From Patients With Uveal Melanoma A Basis for Checklists Daniel Albert, MD; Nasreen Syed, MD; for the Members of the Cancer Committee, College of American Pathologists
More informationWe report the case of a 77-year-old woman in whom choroidal metastasis was the
CLINICOPATHOLOGIC REPORT Choroidal Metastasis as the Initial Manifestation of a Pigmented Neuroendocrine Tumor Ralph C. Eagle, Jr, MD; Hormoz Ehya, MD; Jerry A. Shields, MD; Carol L. Shields, MD We report
More informationLACRIMAL GLAND ADENOMA IN A SHEEP
Bulgarian Journal of Veterinary Medicine, 2017 ONLINE FIRST ISSN 1311-1477; DOI: 10.15547/bjvm.2013 Case report LACRIMAL GLAND ADENOMA IN A SHEEP Summary G. FARJANIKISH 1, A. KHODAKARAM-TAFTI 2 & M. GHANE
More informationAn Evaluation of Xylene-free Processing of Tissues From the Central Nervous System Using the PelorisTM Dual Retort Rapid Tissue Processor
An Evaluation of Xylene-free Processing of Tissues From the Central Nervous System Using the PelorisTM Dual Retort Rapid Tissue Processor Geoffrey Rolls Leica Microsystems, iosystems Division, Melbourne,
More informationCase Study. Monocular Malignant Melanoma
Case Study Monocular Malignant Melanoma Case History A 52 year old Caucasian female presented with a number of naevi on the skin and a right ciliary body malignant melanoma twelve years ago and had an
More informationONCOLOGY. Csaba Bödör. Department of Pathology and Experimental Cancer Research november 19., ÁOK, III.
ONCOLOGY Csaba Bödör Department of Pathology and Experimental Cancer Research 2018. november 19., ÁOK, III. bodor.csaba1@med.semmelweis-univ.hu ONCOLOGY Characteristics of Benign and Malignant Neoplasms
More information