ABOUT US WORLDWIDE DISTRIBUTION ICON KEY STAIN LINE PRODUCT LIST

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3 ABOUT US WORLDWIDE DISTRIBUTION ICON KEY STAIN LINE PRODUCT LIST

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5 BQCKit Company is one of the three differentiated business lines of Bioquochem S.L. We are a Spanish biotechnology company specialized in the design, development and manufacture of biological kits for the measurement of biological parameters. These kits present characteristics of specificity and are geared especially for fields of health, food and cosmetic. All BQCkit Assay Kits are designed, developed and manufactured in Spain by BQCkit team, which is formed by PhDs and researchers from the University of Oviedo with several years of experience. The company strengths are innovation, personalized attention, very competitive prices and short delivery time. As a company involved with science, BQCkit is always in the search of new solutions and products of maximum interest for the customer, trying to find the shorter analysis time, the highest sensitivity and stability and the greater range of linearity of the already existing products in the market. Our main goal is to help researchers in their daily job, this is the reason why our team gives expert technical support being able to solve any problem related to its products. We really value your time, that s why we not only try to answer your enquiries as soon as possible, but also send your orders in a short delivery time. BQCkit has expert technical support being able to solve any problem related to its products and customer s doubts immediately. BQCkit catalogue includes a wide range of assay kits/products, related to different lines such as oxidative stress, cell biology or food analysis, which are constantly growing due to the active work of our R&D department. BQCkit kits are for R&D use only. 5

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7 Available sizes Staining characteristics Time of preparation 7

8 ALCIAN BLUE STAIN KIT KH07001 Alcian Blue 2.5 ph is used to detect acid mucopolysaccharides in tissue samples, that cannot be detected with the habitual method of hematoxylin eosin. Sulphured and carboxylated mucins can be visualized. Nuclear fast red is supplied as a counterstain, to visualize cell nucleus. Nuclei can be visualized as red and mucopolysaccharides in blue 45 min Image 1 Attribution By The Armed Forces Institute of Pathology (AFIP) [Public domain], via Wikimedia Commons GOMORI TRICHROME STAIN KIT KH07002 Gomori trichrome stain is used to demonstrate muscle fiber and cytoplasm in red, nuclei in black and collagen containing connective tissue in blue. Weigert s Hematoxylin and Aniline blue are included to improve staining balance. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining Nuclei can be visualized as black, muscle fiber and cytoplasm in red and collagen containing connective tissue in blue 2 h Image 2. Lung section staining from a wild type C57BL6J mouse. HARRIS MODIFIED, HEMATOXYLIN SOLUTION KH07003 Hematoxylin is frequently used together with Eosin, nuclear fast red, periodic acid or alcian blue. It is also used in immunohistochemical procedures to visualize the end product. This product does not contain mercury. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Nuclei can be visualized as blue-purple min Image 3 Attribution: By No machine-readable author provided. KGH assumed (based on copyright claims). [GFDL ( or CC-BY-SA-3.0 ( via Wikimedia Commons 8

9 MAYER S HEMATOXYLIN AND EOSIN STAIN KIT KH07004 Hematoxylin Eosin is a common staining technique used in a wide range of tissues. Through this method, nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple min Image 4 Attribution By No machine-readable author provided. KGH assumed (based on copyright claims). [GFDL ( or CC-BY-SA-3.0 ( via Wikimedia Commons HARRIS HEMATOXYLIN AND EOSIN STAIN KIT KH07005 Hematoxylin Eosin is a common staining technique used in a wide range of tissues. Through this method, nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple min Image 5 Attribution: By No machine-readable author provided. KGH assumed (based on copyright claims). [GFDL ( or CC-BY-SA-3.0 ( via Wikimedia Commons WEIGERT S IRON HEMATOXYLIN STAIN KIT KH07006 Hematoxylin is frequently used together with Eosin, nuclear fast red, periodic acid or alcian blue. It is also used in immunohistochemical procedures to visualize the end product. This product does not contain mercury. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Nuclei can be visualized as black 1-2 h Image 6 Attribution By Michal Maňas (snek01) [CC BY 2.5 ( from Wikimedia Commons 9

10 MASSON S TRICHROME STAIN KIT KH07007 Masson trichrome stain is used to demonstrate connective tissue elements, collagen and muscle fibers. After staining, collagen and mucin will be coloured in blue, muscle fibers, cytoplasm and keratin in red and the nuclei blue/black. Sample preparation: Formalin fixed, paraffin and frozen samples can be used. After each step, rinsing with tap water and distilled water is required unless specified Collagen and mucin can be visualized as blue, muscle fibers, cytoplasm and keratin in red and the nuclei blue/black. 2 h Image 7 Attribution: Per Nephron [CC BY-SA 3.0 ( o GFDL ( de la Wikimedia Commons MAYER S HEMATOXYLIN SOLUTION KH07008 Hematoxylin is frequently used together with Eosin, nuclear fast red, periodic acid or alcian blue. It is also used in immunohistochemical procedures to visualize the end product. This product does not contain mercury. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Nuclei can be visualized as blue-purple 40 min Image 8 Attribution By No machine-readable author provided. KGH assumed (based on copyright claims). [GFDL ( or CC-BY- SA-3.0 ( via Wikimedia Commons OIL RED O (LIPID STAIN) KIT KH07009 The Oil Red O kit is used to demonstrate adipocites and neutral triglicerides. This is also useful to demonstrate adipogenesis. Fat cells and neutral fat will be coloured in red and the nuclei in blue. Sample preparation: This kit can be used in frozen tissue sections, fresh smears or touch preps. It is recommended the use of saline formalin or Baker s fixative in order to maintain the phospholipids Fat cells and neutral fat can be coloured in red and the nuclei in bluepurple min Image 9 Attribution By M. Oktar Guloglu [CC BY-SA 4.0 ( from Wikimedia Commons 10

11 PERIODIC ACID-SCHIFF (PAS)- ALCIAN BLUE KH07010 PAS-AB technique is used to differentiate between acidic mucins as sialomucins, sulfomucin and neutral epithelial mucin. After staining, acidic mucins will be coloured blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Acidic mucins can be coloured in blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour. 45 min Image 10 Attribution By The Armed Forces Institute of Pathology (AFIP) [Public domain], via Wikimedia Commons PERIODIC ACID-SCHIFF (PAS)- MAYER S HEMATOXYLIN KH07011 Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Mayer s hematoxylin is used to stain cell nuclei. Nuclei can be visualized in violet, simple polysaccharides, neutral mucopolisaccharides, glicolipids and basement membrane in red/purple 60 min Image 11 Attribution By Jerad M Gardner, MD [CC BY-SA 3.0 ( or GFDL ( from Wikimedia Commons PERIODIC ACID-SCHIFF (PAS)- HARRIS HEMATOXYLIN KH07012 Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Harris hematoxylin is used to stain cell nuclei. Nuclei can be visualized in violet, simple polysaccharides, neutral mucopolisaccharides, glicolipids and basement membrane in red/purple 60 min Image 12 Attribution By CoRus13 [CC BY-SA 4.0 ( from Wikimedia Commons 11

12 SCHIFF REAGENT SOLUTION KH07013 Schiffs reagent is used in the staining technique PAS. Periodic Acid Schiff is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. It can be used together with hematoxylin or alcian blue. Only used together with the PAS Stain 60 min Image 13 Attribution By The Armed Forces Institute of Pathology (AFIP) [Public domain], via Wikimedia Commons CRESYL VIOLET SOLUTION 0.1 % KH07014 Cresyl Violet 0,1% is used to demonstrate Nissl substance in the nervous system, X chromatin, Helicobacter, mastocytes and cartilage granules. Neurons are visualized in purple and cell nuclei in blue. Nissl substance in neurons can be visualized as purple and nuclei in blue 10 min Image 14 Attribution By Marika Nosten-Bertrand, Caroline Kappeler, Céline Dinocourt, Cécile Denis, Johanne Germain, Françoise Phan Dinh Tuy, Soraya Verstraeten, Chantal Alvarez, Christine Métin, Jamel Chelly, Bruno Giros, Richard Miles, Antoine Depaulis et Fiona CRESYL VIOLET SOLUTION 1 % KH07015 Cresyl Violet 0,1% is used to demonstrate Nissl substance in the nervous system, X chromatin, Helicobacter, mastocytes and cartilage granules. Neurons are visualized in purple and cell nuclei in blue. Nissl substance in neurons can be visualized as purple and nuclei in blue 10 min Image 15 By Marika Nosten-Bertrand, Caroline Kappeler, Céline Dinocourt, Cécile Denis, Johanne Germain, Françoise Phan Dinh Tuy, Soraya Verstraeten, Chantal Alvarez, Christine Métin, Jamel Chelly, Bruno Giros, Richard Miles, Antoine Depaulis et Fiona Francis [CC 12

13 CRESYL VIOLET SOLUTION 2 % KH07016 Cresyl Violet 0,1% is used to demonstrate Nissl substance in the nervous system, X chromatin, Helicobacter, mastocytes and cartilage granules. Neurons are visualized in purple and cell nuclei in blue. Nissl substance in neurons can be visualized as purple and nuclei in blue 10 min Image 16 By Marika Nosten-Bertrand, Caroline Kappeler, Céline Dinocourt, Cécile Denis, Johanne Germain, Françoise Phan Dinh Tuy, Soraya Verstraeten, Chantal Alvarez, Christine Métin, Jamel Chelly, Bruno Giros, Richard Miles, Antoine Depaulis et Fiona Francis [CC 13

14 STAIN LINE Reference Product Name Size Price ( ) KH KH ALCIAN BLUE STAIN KIT 105,50 232,10 KH KH GOMORI TRICHROME STAIN KIT 146,80 322,90 KH KH HARRIS MODIFIED, HEMATOXYLIN SOLUTION 39,90 87,90 KH KH MAYER S HEMATOXYLIN AND EOSIN STAIN KIT 98,60 216,90 KH KH HARRIS HEMATOXYLIN AND EOSIN STAIN KIT 98,60 216,90 KH KH WEIGERT S IRON HEMATOXYLIN STAIN KIT 66,90 147,50 KH KH MASSON S TRICHROME STAIN KIT 144,20 317,50 KH KH MAYER'S HEMATOXYLIN SOLUTION 39,80 87,60 KH KH OIL RED O (LIPID STAIN) KIT 169,70 373,40 KH KH PERIODIC ACID-SCHIFF (PAS)-ALCIAN BLUE 188.,80 442,10 KH KH PERIODIC ACID-SCHIFF (PAS)-MAYER S HEMATOXYLIN 107,40 236,30 KH KH PERIODIC ACID-SCHIFF (PAS)-HARRIS HEMATOXYLIN 107,40 236,30 KH KH SCHIFF REAGENT SOLUTION 35,90 78,90 KH KH CRESYL VIOLET SOLUTION 0.1% 88,70 195,10 KH KH CRESYL VIOLET SOLUTION 1% 154,80 340,50 KH KH CRESYL VIOLET SOLUTION 2% 207,90 587,

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