John Bell Centre for Innovative Cancer Therapeutics
|
|
- Kathleen Atkins
- 5 years ago
- Views:
Transcription
1 Enhancing Oncolytic Virus Activity by Engineering of Artificial micrornas John Bell Centre for Innovative Cancer Therapeutics Affiliated with Affilié à 1
2 Oncolytic Viruses: A Therapy for Metastatic Cancers? Lung Tumours Targeted Infection Tumour Clearance 2
3 Lessons Learned: Ideal Oncolytic Immunotherapeutic Tumor specific and highly lytic Intravenous Delivery: effective against metastatic disease Robust, long lasting tumor specific adaptive immune response Functional, cytolytic TIL and epitope spreading Complements Immune Checkpoint Inhibitors, IO therapies Strong IP and scalable production 3
4 Bio-prospecting for a potent OV Candidate Bioprospecting Rhabdovirus Screening Enhancement/ Augmentation Recombinant system Virus Isolates In Vitro screening assays (cytotoxicity & replication) Candidates (good tumour cell killers) Recombinant Bioselection In vivo efficacy testing Transfect DNA 293T cells Harvest after hours In vivo toxicity/ biodistribution profiling (naïve and tumour bearing animals) Orthotopic models (brain, lung, breast) Plaque Purify & Amplify Candidates (Safe and Effective)
5 Maraba virus: an ideal oncolytic & vaccine candidate Rhabdovirus Structure Rhabdovirus Life Cycle 11 kb single stranded negative sense RNA genome Matrix protein (M) is involved in budding, silencing host gene expression, and apoptosis Little pre-existing immunity; Cytoplasmic life cycle no genotoxicity; Genetically stable; Fully functionalized recombinant system (mutagenesis, payload); Ease of GMP manufacturing; Strong Patent position; Unique immune-stimulating platform.
6 How to increase potency?
7 Manipulate the Infected Host Cell with amirnas Cell Host and Microbe Sept 2013
8 A B Genome-targeting Sindbis virus-based library (16,000 amirna) Library designed using the Hannon and Elledge algorithm In Collaboration with Dr. tenoever (Mt. Sinai, New York)
9 Large-Scale Genome Screening to Identify Virus Replication Factors P0 of SV-LIB ( unique clones) Identification of host factors that enhance virus pathogenicity Varble et al, 2013
10 The challenges Pancreatic Cancer Highest mortality rate of all major cancers (Survival has not improved in the past 30 years) No curative treatment options Fibrotic nature (Cancer-associated fibroblasts are the predominant component of Pancreatic tumor stroma) P013 P019 P023 Pancreatic cancer patient samples- anti-asma
11 Our Strategy Select/engineer oncolytic viruses that have enhanced capacity to replicate in and kill both Pancreatic cancer cells and the CAFs that support their malignant growth/progression In vivo Serial Passaging on CAFS and PCa Deep sequencing In vitro
12 Project Overview MiaPaca-2 Human pancreatic carcinoma In vitro PanC1 Human Pancreatic/ductal carcinoma In vitro PanC02 Mouse Pancreatic carcinoma In vitro PaCF Fetal Pancreatic Fibroblasts (~CAFs) In vitro U125T Patient-derived CAFs In vitro GM38 Normal Lung Fibroblasts In vitro MiaPaca-2 Human pancreatic carcinoma In vivo MiaPaca-2+ PaCF P009 P014 P020 Human pancreatic carcinoma + Pancreatic Fibroblasts Pancreatic cancer Patient-derived grafts In vivo In vivo In vivo In vivo
13 amirna Activity in Maraba Virus 13
14 MG1 virus-mediated Silencing of Luciferase Arbitrary Luciferase Luminescence MG1-amiRLuc 5 N P M G L MOCK MG1 MG1-shLUC/shGFP p< DOX +DOX Induction Condition MiaPaca-Luciferase cells - Tetracycline ON inducible gene expression system
15 Enrichment of amirnas that enhance Oncolytic Virus Growth in PCa and CAFs Fold increase (relative to Library) GM38 PF MP PC2 MP-IV MP+PaCFs-IV amir-1 amir-2&3 amir-4 amir-5 amir-6 amir-7 amir-8 amir-9 amir-10 In Collaboration with Dr. Boutros (Ontario Institute for Cancer Research, Toronto)
16 Cell death (%) % Cell Death % Cell Death % Cell Death % Cell Death % Cell Death Enriched amirnas confer enhanced OV cytotoxicity 60 PanCO2 MiaPaca-2 PanC VSV-miRGFP VSV-amiR VSV-miRGFP VSV-amiR1` VSV-amiR2 VSV-amiR3 VSV-amiR4 VSV-amiR5 VSV-amiR6 VSV-amiR8 VSV-amiR9 VSV-amiR10 MOCK VSV-miRGFP VSV-amiR1` VSV-amiR2 VSV-amiR3 VSV-amiR4 VSV-amiR5 VSV-amiR6 VSV-amiR8 VSV-amiR9 VSV-amiR10 MOCK HPAFII HPAC PanFIB VSV-amiR2 VSV-amiR3 VSV-amiR4 VSV-amiR5 VSV-amiR6 VSV-amiR8 VSV-amiR9 VSV-amiR10 VSV-miRGFP VSV-amiR1` VSV-amiR2 VSV-amiR3 VSV-amiR4 VSV-amiR5 VSV-amiR6 VSV-amiR8 VSV-amiR9 VSV-amiR10 MOCK VSV-miRGFP VSV-amiR1` VSV-amiR2 VSV-amiR3 VSV-amiR4 VSV-amiR5 VSV-amiR6 VSV-amiR8 VSV-amiR9 VSV-amiR10 MOCK VSV-miRGFP VSV-amiR1` VSV-amiR2 VSV-amiR3 VSV-amiR4 VSV-amiR5 VSV-amiR6 VSV-amiR8 VSV-amiR9 VSV-amiR10 MOCK
17 Mock VSVD51-amiRGFP VSVD51-amiR-6 PanC1 PanCO2 HPAF II
18 Objective: To identify synergistic interactions between virotherapy and synthetic lethality for Pancreatic cancers. Synthetic lethal screens can be used to identify genes or small-molecule compounds to specifically target tumour cells while sparing the normal tissue
19 mirna transfer via exosomes may mediate bystander effect mirna are secreted in exosomes (local environment and systemic delivery) Tumor-associated exosomes determine organotropic metastasis (proven in Breast and Pancreatic cancer patients). Integrin-mediated exosomal tropism and cargoes delivery (Hoshino et al. Nature.2015) Sorting of mirna into exosomes is controlled by short motifs (EXOmotifs) (Villarroya-Beltri et al, Nature communications) Epstein-Barr virus-infected cells secrete exosomes that contain EBV-encoded mirnas (Pegtel et al, PNAS. 2010)
20 Infection increases exosome secretion in pancreatic cell lines HPAC Panc1 OVCAR8 SKOV3 HPAFII kda CD63 Secreted exosomes were concentrated by ultracentifugation. Lane 1 = uninfected Lane 2 = infected with VSV-GFP MOI 0.1
21
Oncolytic virus strategy
Oncolytic viruses Oncolytic virus strategy normal tumor NO replication replication survival lysis Oncolytic virus strategy Mechanisms of tumor selectivity of several, some of them naturally, oncolytic
More informationTumors arise from accumulated genetic mutations. Tumor Immunology (Cancer)
Tumor Immunology (Cancer) Tumors arise from accumulated genetic mutations Robert Beatty MCB150 Mutations Usually have >6 mutations in both activation/growth factors and tumor suppressor genes. Types of
More informationEnhancing Anti-tumour Immunity with Oncolytic Viruses
Enhancing Anti-tumour Immunity with Oncolytic Viruses John Bell Ottawa Hospital Research Institute SUPPORTED BY: NATIONAL CANCER INSTITUTE OF CANADA/CCS THE TERRY FOX FOUNDATION PPG CANADIAN INSTITUTES
More informationExperimental Therapeutics I
Experimental Therapeutics I Mary Hitt 5142 Katz Group Centre mhitt@ualberta.ca; or Mary.Hitt@albertahealthservices.ca 1 Specific Topics for Today Preclinical and clinical testing Gene therapy Nonviral
More informationBIT 120. Copy of Cancer/HIV Lecture
BIT 120 Copy of Cancer/HIV Lecture Cancer DEFINITION Any abnormal growth of cells that has malignant potential i.e.. Leukemia Uncontrolled mitosis in WBC Genetic disease caused by an accumulation of mutations
More informationOncolytic Viruses as a Potential Approach to Eliminate the HIV Reservoir
Oncolytic Viruses as a Potential Approach to Eliminate the HIV Reservoir Costiniuk CT, Côté SC, Al-Ghazawi FM, Carrasco-Medina L,Young CD, & Angel JB University of Ottawa, November 12 th, 2012 HIV Reservoirs
More informationArming the Oncolytic Virus Enadenotucirev to Develop Tumor-Localized Combination Immunotherapeutics. Charles Q. Morris MBChB MRCP(UK)
Arming the Oncolytic Virus Enadenotucirev to Develop Tumor-Localized Combination Immunotherapeutics Charles Q. Morris MBChB MRCP(UK) 1 Enadenotucirev (EnAd): Developed using directed evolution EnAd Start
More informationOncolytic viruses for cancer treatment
Oncolytic viruses for cancer treatment Interdisciplinary Technical Journal Club: Special Series on Laboratory Animal Science Dr. Katrin Frauenknecht Institute of Neuropathology University Hospital Zurich
More informationCurrent Strategies in HIV-1 Vaccine Development Using Replication-Defective Adenovirus as a Case Study
Note: I have added some clarifying comments to the slides -- please click on Comments under View to see them. Current Strategies in HIV-1 Vaccine Development Using Replication-Defective Adenovirus as a
More informationCOURSE: Medical Microbiology, PAMB 650/720 - Fall 2008 Lecture 16
COURSE: Medical Microbiology, PAMB 650/720 - Fall 2008 Lecture 16 Tumor Immunology M. Nagarkatti Teaching Objectives: Introduction to Cancer Immunology Know the antigens expressed by cancer cells Understand
More informationTumor Immunology. Tumor (latin) = swelling
Tumor Immunology Tumor (latin) = swelling benign tumor malignant tumor Tumor immunology : the study of the types of antigens that are expressed by tumors how the immune system recognizes and responds to
More informationOncolytic Virotherapy Summit Novel stroma targeted and microenvironment-inducible oncolytic adenoviruses (OAVs)
Oncolytic Virotherapy Summit 2016 Novel stroma targeted and microenvironment-inducible oncolytic adenoviruses (OAVs) Osvaldo L. Podhajcer, Ph.D. Superior Researcher CONICET (Consejo Nacional de Investigaciones
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationIntrinsic cellular defenses against virus infection
Intrinsic cellular defenses against virus infection Detection of virus infection Host cell response to virus infection Interferons: structure and synthesis Induction of antiviral activity Viral defenses
More informationLAMPvax DNA Vaccines as Immunotherapy for Cancer - Three Case Studies
LAMPvax DNA Vaccines as Immunotherapy for Cancer - Three Case Studies Cancer immunotherapy has emerged as a clinically validated tool for fighting certain kinds of cancers. These therapeutic cancer vaccines
More informationOncolytic Virotherapy: Targeting Cancer Stem Cells
Oncolytic Virotherapy: Targeting Cancer Stem Cells Cancer Stem Cells (CSCs) or Cancer Initiating Cells (CICs) A consensus of five defining criteria has been established to affirm the existence of CICs:
More informationSection Lectures: Immunology/Virology Time: 9:00 am 10:00 am LRC 105 A & B
Section Director: Cliff Bellone, Ph.D. Office: Doisy Hall - R 405 Phone: 577-8449 E-Mail: bellonec@slu.edu Lecturers: James Swierkosz, Ph.D. Office: Medical School Rm. 412 Phone: 577-8430 E-Mail: swierkoszje@slu.edu
More informationThe next steps for effective cancer immunotherapy and viral vaccines. Peter Selby FACP(UK)
The next steps for effective cancer immunotherapy and viral vaccines Peter Selby FACP(UK) Richard Alan Steve Sasha Matt Nav Vile Melcher Griffin Zougman Bentham Vasudev Adel Nick Gemma Liz Samson Hornigold
More informationVIRUSES. Biology Applications Control. David R. Harper. Garland Science Taylor & Francis Group NEW YORK AND LONDON
VIRUSES Biology Applications Control David R. Harper GS Garland Science Taylor & Francis Group NEW YORK AND LONDON vii Chapter 1 Virus Structure and 2.2 VIRUS MORPHOLOGY 26 Infection 1 2.3 VIRAL CLASSIFICATION
More informationImmune surveillance hypothesis (Macfarlane Burnet, 1950s)
TUMOR-IMMUNITÄT A.K. Abbas, A.H. Lichtman, S. Pillai (6th edition, 2007) Cellular and Molecular Immunology Saunders Elsevier Chapter 17, immunity to tumors Immune surveillance hypothesis (Macfarlane Burnet,
More informationVirion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics
Hepadnaviruses Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics Hepatitis viruses A group of unrelated pathogens termed hepatitis viruses cause the vast majority
More informationInfluenza A Virus Transmission Bottlenecks Are Defined by Infection Route and Recipient Host
Cell Host & Microbe, Volume 16 Supplemental Information Influenza A Virus Transmission Bottlenecks Are Defined by Infection Route and Recipient Host Andrew Varble, Randy A. Albrecht, Simone Backes, Marshall
More informationLESSON 4.4 WORKBOOK. How viruses make us sick: Viral Replication
DEFINITIONS OF TERMS Eukaryotic: Non-bacterial cell type (bacteria are prokaryotes).. LESSON 4.4 WORKBOOK How viruses make us sick: Viral Replication This lesson extends the principles we learned in Unit
More informationOncolytic Viruses: Reovirus
T S X : O N C N A S D A Q : O N C Y International Society for Biological Therapy of Cancer 2008 Oncology Biologics Development Primer Oncolytic Viruses: Reovirus REOLYSIN - mode of action REOLYSIN contains
More informationShiv Pillai Ragon Institute, Massachusetts General Hospital Harvard Medical School
CTLs, Natural Killers and NKTs 1 Shiv Pillai Ragon Institute, Massachusetts General Hospital Harvard Medical School CTL inducing tumor apoptosis 3 Lecture outline CD8 + Cytotoxic T lymphocytes (CTL) Activation/differentiation
More informationTMAC Affimer Drug Conjugates
TMAC Affimer Drug Conjugates A novel and proprietary class of immuno-oncology active, dual mode of action drug conjugates PRS 001 Immune-checkpoint Therapies: Improving Patient Response Rates Despite great
More informationIMMUNOTHERAPY IMMUNOTHERAPY. CHI Immuno-oncology Summit Boston. 27 th August 2018
NEXT NEXT GENERATION GENERATION ONCOLYTIC ONCOLYTIC IMMUNOTHERAPY IMMUNOTHERAPY CHI Immuno-oncology Summit Boston 27 th August 2018 2 Safe harbor Any statements contained herein that are not statements
More informationUnderstanding the T cell response to tumors using transnuclear mouse models
Understanding the T cell response to tumors using transnuclear mouse models Stephanie Dougan Dana-Farber Cancer Institute Boston, MA Presenter Disclosure Information Stephanie Dougan The following relationships
More informationOxford Expression Technologies Ltd
Oxford Expression Technologies Ltd Founded in 2007 as a spin out from Oxford Brookes University and Natural Environment Research Council Technology based on the insect baculovirus expression vectors (BEVs)
More informationSupplementary Figure 1 IL-27 IL
Tim-3 Supplementary Figure 1 Tc0 49.5 0.6 Tc1 63.5 0.84 Un 49.8 0.16 35.5 0.16 10 4 61.2 5.53 10 3 64.5 5.66 10 2 10 1 10 0 31 2.22 10 0 10 1 10 2 10 3 10 4 IL-10 28.2 1.69 IL-27 Supplementary Figure 1.
More informationTargeting Human Glioblastoma Cells: Comparison of Nine Viruses with Oncolytic Potential
JOURNAL OF VIROLOGY, May 2005, p. 6005 6022 Vol. 79, No. 10 0022-538X/05/$08.00 0 doi:10.1128/jvi.79.10.6005 6022.2005 Copyright 2005, American Society for Microbiology. All Rights Reserved. Targeting
More informationTITLE: Construction of a Vesicular Stomatitis virus Expressing Both a Fusogenic Glycoprotein and IL-12: A Novel Vector for Prostate Cancer Therapy
AD AWARD NUMBER: W8XWH-4--47 TITLE: Construction of a Vesicular Stomatitis virus Expressing Both a Fusogenic Glycoprotein and IL-2: A Novel Vector for Prostate Cancer Therapy PRINCIPAL INVESTIGATOR: Simon
More informationReviewers' comments: Reviewer #1 (Remarks to the Author):
Reviewers' comments: Reviewer #1 (Remarks to the Author): In the manuscript Rational Combination of CXCL11-Expressing Oncolytic Virus and PD-L1 Blockade Works Synergistically to Enhance Therapeutic Efficacy
More informationPhysicsAndMathsTutor.com. Question Number. 1. prevents viruses attaching to {uninfected / eq} host cells / eq ; 2. by binding to receptors / eq ;
1(a) 1. prevents viruses attaching to {uninfected / eq} host cells / eq ; 2. by binding to receptors / eq ; 3. (therefore) preventing virus from entering cell / eq ; 4. (therefore) viruses cannot replicate
More informationRXi Pharmaceuticals. Immuno-Oncology World Frontiers Conference. January 23, 2018 NASDAQ: RXII. Property of RXi Pharmaceuticals
RXi Pharmaceuticals Immuno-Oncology World Frontiers Conference January 23, 2018 NASDAQ: RXII Forward Looking Statements This presentation contains forward-looking statements within the meaning of the Private
More informationAn Epstein-Barr virus-encoded microrna targets PUMA to promote host cell survival
An Epstein-Barr virus-encoded microrna targets to promote host cell survival The Journal of Experimental Medicine 205(11): 2551-2560, 2008. 1 Elizabeth Yee-Wai Choy, Kam-Leung Siu, Kin-Hang Kok, Raymond
More informationAmy Lin, Ph.D. November 14, 2018
PD L1 checkpoint blockade using a single chain variable fragment targeting PD L1 delivered by retroviral replicating vector (Toca 521) enhances anti tumor effect in murine cancer models Amy Lin, Ph.D.
More informationThis training module is required for all personnel listed on an IBC protocol that describes work utilizing viral vectors (both replication competent
This training module is required for all personnel listed on an IBC protocol that describes work utilizing viral vectors (both replication competent and incompetent) regardless of the biosafety level used
More informationHIV Immunopathogenesis. Modeling the Immune System May 2, 2007
HIV Immunopathogenesis Modeling the Immune System May 2, 2007 Question 1 : Explain how HIV infects the host Zafer Iscan Yuanjian Wang Zufferey Abhishek Garg How does HIV infect the host? HIV infection
More informationTumor Immunology. Wirsma Arif Harahap Surgical Oncology Consultant
Tumor Immunology Wirsma Arif Harahap Surgical Oncology Consultant 1) Immune responses that develop to cancer cells 2) Escape of cancer cells 3) Therapies: clinical and experimental Cancer cells can be
More informationLesson 19 Study Guide: Medical Biotechnology Cancer Treatment
URI CMB 190 Issues in Biotechnology Lesson 19 Study Guide: Medical Biotechnology Cancer Treatment 11. There have been genes that have been identified to be associated with certain types of cancer. Microarrays
More informationChapter 4 Cellular Oncogenes ~ 4.6 -
Chapter 4 Cellular Oncogenes - 4.2 ~ 4.6 - Many retroviruses carrying oncogenes have been found in chickens and mice However, attempts undertaken during the 1970s to isolate viruses from most types of
More informationBiomarkers: An approach to targeting SMARCB1-deficient sarcomas
Biomarkers: An approach to targeting SMARCB1-deficient sarcomas Description The basis for dual targeting SMARCB1-deficient sarcomas with inhibitors towards PDGFRalpha and FGFR1. Key publications Wong et
More informationTITLE: Systemic Oncolytic Cytokine HSV Therapy of Prostate Cancer. CONTRACTING ORGANIZATION: Massachusetts General Hospital Boston, MA
AD Award Number: W81XWH-05-1-0367 TITLE: Systemic Oncolytic Cytokine HSV Therapy of Prostate Cancer PRINCIPAL INVESTIGATOR: Susan Varghese, Ph.D. CONTRACTING ORGANIZATION: Massachusetts General Hospital
More informationJune IMMUNE DESIGN The in vivo generation of cytotoxic CD8 T cells (CTLs)
June 2015 IMMUNE DESIGN The in vivo generation of cytotoxic CD8 T cells (CTLs) 1 Forward-looking Statements This presentation contains forward-looking statements with respect to, among other things, our
More informationHerpesviruses. Virion. Genome. Genes and proteins. Viruses and hosts. Diseases. Distinctive characteristics
Herpesviruses Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics Virion Enveloped icosahedral capsid (T=16), diameter 125 nm Diameter of enveloped virion 200 nm Capsid
More informationMedical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University
Medical Virology Immunology Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University Human blood cells Phases of immune responses Microbe Naïve
More informationTherapeutic efficacy of MUC1- specific CTL and CD137 costimulation. mammary cancer model. Pinku Mukherjee & Sandra Gendler
Therapeutic efficacy of MUC1- specific CTL and CD137 costimulation in a spontaneous mammary cancer model Pinku Mukherjee & Sandra Gendler Goal of Immunotherapy Boosting the low level anti-tumor immune
More informationLecture 2: Virology. I. Background
Lecture 2: Virology I. Background A. Properties 1. Simple biological systems a. Aggregates of nucleic acids and protein 2. Non-living a. Cannot reproduce or carry out metabolic activities outside of a
More informationVIRUSES. 1. Describe the structure of a virus by completing the following chart.
AP BIOLOGY MOLECULAR GENETICS ACTIVITY #3 NAME DATE HOUR VIRUSES 1. Describe the structure of a virus by completing the following chart. Viral Part Description of Part 2. Some viruses have an envelope
More informationIMMUNOTHERAPY FOR CANCER A NEW HORIZON. Ekaterini Boleti MD, PhD, FRCP Consultant in Medical Oncology Royal Free London NHS Foundation Trust
IMMUNOTHERAPY FOR CANCER A NEW HORIZON Ekaterini Boleti MD, PhD, FRCP Consultant in Medical Oncology Royal Free London NHS Foundation Trust ASCO Names Advance of the Year: Cancer Immunotherapy No recent
More informationAntiviral Drugs Lecture 5
Antiviral Drugs Lecture 5 Antimicrobial Chemotherapy (MLAB 366) 1 Dr. Mohamed A. El-Sakhawy 2 Introduction Viruses are microscopic organisms that can infect all living cells. They are parasitic and multiply
More informationB19, see Parvovirus B19 Bone marrow, gene transfer with parvovirus. Erythrovirus, see Parvovirus B19, Simian parvovirus
... Subject Index Adeno-associated virus Cap and genome encapsidation 87 DNA integration homologous recombination 90, 91 latency vs replication 77, 78 mechanism 79 requirements 78, 79 site in human genome
More informationGene Vaccine Dr. Sina Soleimani
Gene Vaccine Dr. Sina Soleimani Human Viral Vaccines Quality Control Laboratory (HVVQC) Titles 1. A short Introduction of Vaccine History 2. First Lineage of Vaccines 3. Second Lineage of Vaccines 3. New
More informationViral Vectors In The Research Laboratory: Just How Safe Are They? Dawn P. Wooley, Ph.D., SM(NRM), RBP, CBSP
Viral Vectors In The Research Laboratory: Just How Safe Are They? Dawn P. Wooley, Ph.D., SM(NRM), RBP, CBSP 1 Learning Objectives Recognize hazards associated with viral vectors in research and animal
More informationOncology Program. Chlorotoxin Technology Platform. Corporate Partnering Opportunities
TransMolecular, Inc. A Neuroscience Biotechnology Company Oncology Program Chlorotoxin Technology Platform Corporate Partnering Opportunities Background TransMolecular, Inc. (TMI) is a neuroscience biotechnology
More informationSynthetic Genomics and Its Application to Viral Infectious Diseases. Timothy Stockwell (JCVI) David Wentworth (JCVI)
Synthetic Genomics and Its Application to Viral Infectious Diseases Timothy Stockwell (JCVI) David Wentworth (JCVI) Outline Using informatics to predict drift (strain selection) Synthetic Genomics: Preparedness
More informationDetermination of the temporal pattern and importance of BALF1 expression in Epstein-Barr viral infection
Determination of the temporal pattern and importance of BALF1 expression in Epstein-Barr viral infection Melissa Mihelidakis May 6, 2004 7.340 Research Proposal Introduction Apoptosis, or programmed cell
More information2010 ASCO Annual Meeting Chicago, IL June 4 - June 8, 2010
2010 ASCO Annual Meeting Chicago, IL June 4 - June 8, 2010 Abstract ID: 2546 Poster Board #: 1H A Phase I/II Study of Intravenous Rexin-G and Reximmune-C for Cancer Immunotherapy: The GeneVieve Protocol
More informationBiotechnology-Based Vaccines. Dr. Aws Alshamsan Department of Pharmaceutics Office: AA87 Tel:
Biotechnology-Based Vaccines Dr. Aws Alshamsan Department of Pharmaceutics Office: AA87 Tel: 4677363 aalshamsan@ksu.edu.sa Objectives of this lecture By the end of this lecture you will be able to: 1.
More information2016 ONCOLYTIC VIRUS IMMUNOTHERAPY Part of CHI s
MEETING REPORT: 2016 ONCOLYTIC VIRUS IMMUNOTHERAPY Part of CHI s www.immuno-oncologysummit.com By Cole Peters and Fares Nigim, M.D. from Mass General Hospital - Harvard Medical School Cambridge Healthtech
More informationThird line of Defense
Chapter 15 Specific Immunity and Immunization Topics -3 rd of Defense - B cells - T cells - Specific Immunities Third line of Defense Specific immunity is a complex interaction of immune cells (leukocytes)
More informationVIROLOGY. Engineering Viral Genomes: Retrovirus Vectors
VIROLOGY Engineering Viral Genomes: Retrovirus Vectors Viral vectors Retrovirus replicative cycle Most mammalian retroviruses use trna PRO, trna Lys3, trna Lys1,2 The partially unfolded trna is annealed
More informationIN VIVO STUDIES ON VIRAL VIRULENCE
IN VIVO STUDIES ON VIRAL VIRULENCE M.Phil student: Emily TSUI Supervisor: Professor Paul K.S Chan Department of Microbiology, CUHK Date: 15th Dec, 2014 Viral Virulence Capacity of a virus to cause disease
More informationA Schematic Of The Life Cycle Of The
A Schematic Of The Life Cycle Of The Lymphocytes Involved A wonderful and informative website to visit is from Johns Hopkins Medical Center Called "The Body". The ability of these viruses to infect lymphocytes
More informationAdaptive Immunity: Specific Defenses of the Host
17 Adaptive Immunity: Specific Defenses of the Host SLOs Differentiate between innate and adaptive immunity, and humoral and cellular immunity. Define antigen, epitope, and hapten. Explain the function
More informationImmunity to Viruses. Patricia Fitzgerald-Bocarsly September 25, 2008
Immunity to Viruses Patricia Fitzgerald-Bocarsly September 25, 2008 The Immune System Deals with a Huge Range of Pathogens Roitt, 2003 Immune Responses to Viruses Viruses are dependent on the host cell
More informationBreast cancer. Risk factors you cannot change include: Treatment Plan Selection. Inferring Transcriptional Module from Breast Cancer Profile Data
Breast cancer Inferring Transcriptional Module from Breast Cancer Profile Data Breast Cancer and Targeted Therapy Microarray Profile Data Inferring Transcriptional Module Methods CSC 177 Data Warehousing
More informationOncolytic Immunotherapy: A Local and Systemic Antitumor Approach
Oncolytic Immunotherapy: A Local and Systemic Antitumor Approach Oncolytic immunotherapy Oncolytic immunotherapy the use of a genetically modified virus to attack tumors and induce a systemic immune response
More informationWhat is influenza virus? 13,000 base RNA genome: 1/ the size of the human genome
What is influenza virus? 13,000 base RNA genome: 1/246153 the size of the human genome CDC Principles of Virology, 4e Neumann et al. Nature. 2009. Influenza virus is one of the most deadly viral pathogens
More informationNew Small and Versatile Reporter Technologies for Challenging Applications in Virology. Robert Brazas, Ph.D.
New Small and Versatile Reporter Technologies for Challenging Applications in Virology Robert Brazas, Ph.D. Presentation Overview Small size of NanoLuc Luciferase is advantageous Agenda Building a better
More informationDevelopment of New Treatment Modalities Oncolytic Viruses and Nanotechnique
Development of New Treatment Modalities Oncolytic Viruses and Nanotechnique By Professor Kjell Öberg, M.D., Ph.D. Dept. of Endocrine Oncology, University Hospital, Uppsala, Sweden Nashville Oct. 2011 Hallmarks
More informationViruses Tomasz Kordula, Ph.D.
Viruses Tomasz Kordula, Ph.D. Resources: Alberts et al., Molecular Biology of the Cell, pp. 295, 1330, 1431 1433; Lehninger CD Movie A0002201. Learning Objectives: 1. Understand parasitic life cycle of
More informationMetastasis progression
Metastasis progression Mieloma multiplo Linear Progression Cancer cells disseminate through the organism after acquiring metastatic features inside the primary cancer Parallel progression Cancer cells
More information1. Overview of Adaptive Immunity
Chapter 17A: Adaptive Immunity Part I 1. Overview of Adaptive Immunity 2. T and B Cell Production 3. Antigens & Antigen Presentation 4. Helper T cells 1. Overview of Adaptive Immunity The Nature of Adaptive
More information19/06/2013. Viruses are not organisms (do not belong to any kingdom). Viruses are not made of cells, have no cytoplasm, and no membranes.
VIRUSES Many diseases of plants and animals are caused by bacteria or viruses that invade the body. Bacteria and viruses are NOT similar kinds of micro-organisms. Bacteria are classified as living organisms,
More informationHuman Cytomegalovirus (HCMV) Immediate early proteins, gene expression and signaling
Viruses, Cells and Disease November 13, 2008 Human Cytomegalovirus (HCMV) Immediate early proteins, gene expression and signaling Dr. Hua Zhu ICPH E350D UMDNJ - New Jersey Medical School 973-972-4483 X
More informationLESSON 4.6 WORKBOOK. Designing an antiviral drug The challenge of HIV
LESSON 4.6 WORKBOOK Designing an antiviral drug The challenge of HIV In the last two lessons we discussed the how the viral life cycle causes host cell damage. But is there anything we can do to prevent
More informationC. Incorrect! MHC class I molecules are not involved in the process of bridging in ADCC.
Immunology - Problem Drill 13: T- Cell Mediated Immunity Question No. 1 of 10 1. During Antibody-dependent cell mediated cytotoxicity (ADCC), the antibody acts like a bridge between the specific antigen
More informationReceived 3 September 2002/Accepted 15 January 2003
JOURNAL OF VIROLOGY, Apr. 2003, p. 4646 4657 Vol. 77, No. 8 0022-538X/03/$08.00 0 DOI: 10.1128/JVI.77.8.4646 4657.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved. Ability of
More informationBBS 2711 Virology. Virus Vaccines
BBS 2711 Virology Virus Vaccines Dr Paul Young, Department of Microbiology & Parasitology. p.young@mailbox.uq.edu.au Virus Vaccines First vaccine developed by Jenner in late 1700's against smallpox virus
More informationThe Wistar Institute is an international leader in biomedical
A LEADER IN RESEARCH The Wistar Institute is an international leader in biomedical research with special expertise in cancer, immunology and infectious disease research. The Institute works actively to
More informationFIG S1 Examination of eif4b expression after virus infection. (A) A549 cells
Supplementary Figure Legends FIG S1 Examination of expression after virus infection. () 549 cells were infected with herpes simplex virus (HSV) (MOI = 1), and harvested at the indicated times, followed
More informationPotential Role of Human Endogenous Retrovirus K102 (HERV-K102) Particles in Resistance to HIV-1 Transmission
Potential Role of Human Endogenous Retrovirus K102 (HERV-K102) Particles in Resistance to HIV-1 Transmission M. Laderoute, L. Larocque, A. Giulivi, K.R. Fowke, F.A. Plummer & F. Diaz-Mitoma As presented
More informationBioactivity Assays: Putting the Puzzle Together
Bioactivity Assays: Putting the Puzzle Together Dr. Ulrike Herbrand Department for Biosafety & Bioassay Services Charles River Biologics Testing Solutions Outline General Remarks MoA reflecting bioassays
More informationMeasles virus vaccine induces oncolysis of tumor cells and activates immune responses.
Measles virus vaccine induces oncolysis of tumor cells and activates immune responses. Marc Grégoire*, J.-F. Fonteneau, N. Boisgerault, J.-B. Guillerme and F. Tangy. * INSERM, Nantes, F-44, France. Pasteur
More informationEBV infection B cells and lymphomagenesis. Sridhar Chaganti
EBV infection B cells and lymphomagenesis Sridhar Chaganti How EBV infects B-cells How viral genes influence the infected B cell Differences and similarities between in vitro and in vivo infection How
More informationIMMUNOTHERAPY IMMUNOTHERAPY. 37th Annual JPMorgan Healthcare Conference
NEXT-GENERATION NEXT GENERATION ONCOLYTIC ONCOLYTIC IMMUNOTHERAPY IMMUNOTHERAPY 37th Annual JPMorgan Healthcare Conference 2 Safe harbor Any statements contained herein that are not statements of historical
More informationCellecta Overview. Started Operations in 2007 Headquarters: Mountain View, CA
Cellecta Overview Started Operations in 2007 Headquarters: Mountain View, CA Focus: Development of flexible, scalable, and broadly parallel genetic screening assays to expedite the discovery and characterization
More informationChapter 19: The Genetics of Viruses and Bacteria
Chapter 19: The Genetics of Viruses and Bacteria What is Microbiology? Microbiology is the science that studies microorganisms = living things that are too small to be seen with the naked eye Microorganisms
More informationFor personal use only
Cancer killing viruses Oncolytic Virotherapeutics Bryan Dulhunty Managing Director Bell Potter Life Sciences Conference Building a Global Profile May 2102 bryan.dulhunty@viralytics.com www.viralytics.com
More informationCANCER IMMUNOPATHOLOGY. Eryati Darwin Faculty of Medicine Andalas University
CANCER IMMUNOPATHOLOGY Eryati Darwin Faculty of Medicine Andalas University Padang 18 Mei 2013 INTRODUCTION Tumor: cells that continue to replicate, fail to differentiate into specialized cells, and become
More informationEBV Infection and Immunity. Andrew Hislop Institute for Cancer Studies University of Birmingham
EBV Infection and Immunity Andrew Hislop Institute for Cancer Studies University of Birmingham EBV Introduction Large ds DNA virus Spread by saliva contact Lifelong infection Predominantly B-lymphotropic
More informationExploring Immunotherapies: Beyond Checkpoint Inhibitors
Exploring Immunotherapies: Beyond Checkpoint Inhibitors Authored by: Jennifer Dolan Fox, PhD VirtualScopics (Now part of BioTelemetry Research) jennifer_fox@virtualscopics.com +1 585 249 6231 Introduction
More informationCloudbreak. March Cidara Therapeutics
Cloudbreak March 2019 Cidara Therapeutics 2019 0 Forward-Looking Statements These slides and the accompanying oral presentation contain forward-looking statements within the meaning of the Private Securities
More informationSTRUCTURE, GENERAL CHARACTERISTICS AND REPRODUCTION OF VIRUSES
STRUCTURE, GENERAL CHARACTERISTICS AND REPRODUCTION OF VIRUSES Introduction Viruses are noncellular genetic elements that use a living cell for their replication and have an extracellular state. Viruses
More informationCloudbreak. January Cidara Therapeutics
Cloudbreak January 2019 Cidara Therapeutics 2019 0 Forward-Looking Statements These slides and the accompanying oral presentation contain forward-looking statements within the meaning of the Private Securities
More informationEffector Mechanisms of Cell-Mediated Immunity
Effector Mechanisms of Cell-Mediated Immunity Dr. Julia Rempel Section of Hepatology 789-3825 jdrempel@cc.umanitoba.ca 804D JBRC Topics: I. Types of Cell-Mediated Immunity II. Migration of Effector T Lymphocytes
More information