Three and Four Mass Spectrometers t in Parallel for Lipid Analysis How Many Instruments are Enough?

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1 Three and Four Mass Spectrometers t in Parallel l for Lipid Analysis How Many Instruments are Enough? Wm. Craig Byrdwell Food Composition and Methods Development Laboratory USDA, ARS, Beltsville Human NutritionResearch Center Beltsville, MD Byrdwell.com/Ghent2013.ppt Byrdwell.com/Ghent2013_iPad.ppt

2 Why Parallel Mass Spectrometry? So many experiments to perform So Little Time! (and Sample!)

3 Why Parallel Mass Spectrometry? Arpino, P.J., Trends Anal. Chem., 1:154 (1982).

4 Why Parallel Mass Spectrometry? Three primarytypes of Atmospheric Pressure Ionization (API) techniques for LC MS: Atmospheric Pressure Chemical Ionization (APCI) Electrospray Ionization (ESI) Atmospheric Pressure Photoionization i ti (APPI) E h id Vl bl b C l Each provides Valuable, but Complementary information

5 Atmospheric Pressure Ionization (API) AtmosphericPressureChemical Ionization (APCI) Relatively simple spectra Produces d some, but maybe not a lot, of [M+H] + Often produces major fragments as base peaks Produces d some ACN adducts ( chemical ionization ) i Some, but minimal, discrimination between classes Substantial b t ti ldependence d of [M+H] + on the degree of unsaturation in triacylglycerols (TAGs)

6 Atmospheric Pressure Ionization (API) Atmospheric Pressure Chemical Ionization (APCI) OOS [M+H RCOOH] + [M+H] + = [DAG] + [M+H] + [M+H RCOOH] + = [DAG] + [M+89] + [M+89] + [M+29] + [M+29] + QTrap 5500

7 Atmospheric Pressure Ionization (API) Atmospheric Pressure Chemical Ionization (APCI) OOS [LL] + [OS] + [OL] [OO] + QTrap 5500

8 Atmospheric Pressure Ionization (API) Atmospheric Pressure Chemical Ionization (APCI) [M+H] + [M+H RCOOH] + OOS [DAG] + [M+H RCOOH] + [DAG] + [M+H] + [M+89] + [M+89] + TSQ 7000

9 Atmospheric Pressure Ionization (API) Atmospheric Pressure Chemical Ionization (APCI) [LL] + [OL] + [OS] + OOS [OO] + TSQ 7000

10 Atmospheric Pressure Ionization (API) Electrospray Ionization (ESI) Produces mostly [M+X] +, where X is adduct (e.g., NH + 4+ from NH 4 OCOH) Except: Produces [M+H] + from phospholipids Produces minimal fragmentation Best suited to producing precursors for MS/MS Substantial discrimination between classes Requires q electrolyte (LC or via tee)

11 Atmospheric Pressure Ionization (API) Electrospray Ionization (ESI) [M+NH 4 ] + [M+NH 4 ] + OOS [M+H RCOOH] + [DAG] + [M+H RCOOH] + [DAG] + [2M+NH 4 ] + LCQ Deca XP

12 Atmospheric Pressure Ionization (API) Electrospray Ionization (ESI) [OL] + [OS] + OOS [LL] + [OO] + LCQ Deca XP

13 Triple Parallel MS Instrumentation A/D #2 Solvents de gasser Pump A/S A/D CAD UV ELSD APCI A/S Therm Column Therm Agilent 1200 TSQ 7000 QTrap5500 ESI Solvents de gasser APCI LCQ Deca XP Syringe Pump HP 1050

14 Oil filled Vitamin D 3 Supplement Gelcaps

15 UV 265 nm Isocratic MeOH for Vitamin D 2, D 3 Vit. D 3 Triacylglycerols Vit. D 2 ACN/DCM Gradient for Triacylglycerols Polyunsaturated Saturated

16 UV Full Scan Chromatogram

17 APCI MS on QTrap 5500 TIC TIC Vit. D SIM x12 MRM Avg. Spec.

18 APCI MS on QTrap 5500 TIC LLL LLP OOL POL OOO LLLn OLLn PLLn POLn PPL SLO POP OOS OOP DAG POS OOA POA+SSO OOLg = OO 24:0 [OLg] + [OO] + [M+H] + OOCe = OO 26:0 [OCe] + [OO] + [M+H] +

19 MS and Aux. Det. Chromatograms APCI MS OOO ESI MS OOL OOO ELSD LLP LLL OO OP POL OOP OOL LLP POL LLL CAD DAG POP POP OOS LLLn POS OOS DAG LL LLn POS TSQ 7000 LCQ Deca XP

20 APCI MS on TSQ 7000 TIC DAG LLL OOO OOL POL LLP PPL OOP POPOOS SLO POS OOA D 3 EIC of Vit. D 2 & D 3 D 2 OOB = OO 22:0 [OO] + [OB] + [M+H] + POLg [OLg] + [PO] + [PLg] + [M+H] + OOCe = OO 26:0 [OO] + [OCe] + [M+H] +

21 ESI MS on LCQ Deca XP TIC DAG LLL OOL OOO LLP POL OOP PPL SLO POPOOS POS PPL OOA Full scans MS/MS scans MS 3 scans LLL + OLLn [LL] + [M+NH 4 ] + [LL] + [M+H] + [LL]+ [M+H H 2 O] + PPL [M+NH 4 ] + [PL] + [PP] + [PL] + [PP] + [M+H] + OOB [M+NH 4 ] + [OO] + [OB]+ [OO] +[OB]+ [M+H] + MS MS/MS MS 3 ~APCI MS

22 Atmospheric Pressure Ionization (API) Atmospheric Pressure Photoionization i ti (APPI) Very similar spectra to APCI MS Produces some, but maybe not a lot, of [M+H] + Often produces major fragments as base peaks More discrimination between classes than APCI R i d t f t li id (A t Requires dopant for most lipids (Acetone or ul/min)

23 Atmospheric Pressure Ionization (API) Atmospheric Pressure Photoionization (APPI) OOS [M+H] + [DAG] + [M+H RCOOH] + [DAG] + [M+H] + QTrap 4000

24 Atmospheric Pressure Ionization (API) Atmospheric Pressure Photoionization (APPI) [OL] OOS + [OS] + [LL] + [OO] + QTrap 4000

25 Quadruple Parallel MS Instrumentation Agilent 1200 Solvents de gasser A/D #2 A/D ELSD APCI Pump CAD A/S A/S Therm UV Column Therm TSQ 7000 APPI Solvents Agilent 1290 APCI ESI LCQ Deca XP Syringe Pump TSQ Vantage EMR QTrap 4000

26 APCI MS on TSQ Vantage TIC Full Scan TIC EIC Vit. D SIM x5 MRM Avg. Spec.

27 APCI MS on TSQ Vantage TIC OOO OOP DAG LLL OLL Ln LLP OLO POL POP OOS POS OOA POA Full Scan TIC OOLg = OO 24:0 [OO] + [OLg] + [M+H] + OOCe = OO 26:0 [OO] + [OCe] + [M+H] +

28 APCI MS on TSQ7000 TIC OOO DAG LLL OLLn LLP OLO POL OOP OOS POP POSOOA POA EIC for Vit. D D 2 D 3 [M+H] + OOP [OP] + [OO] + [M+H] + [OL] + [LL] + OLO [M+H] + [OS] [OL] + + OOS [OO] + [M+H] +

29 APCI MS on TSQ7000 TIC OOO DAG LLL OLLn LLP OLO POL OOP OOS POP POSOOA POA EIC for [OO] + O DAG L Po P S [OL] + [LL] + OOP [OP] + [OO] + OLO [OL] + [OS] + OOS [OO] + [OO] +

30 ESI MS on QTrap 4000 TIC DAG LLL OLL Ln LLP * * * OOO OOP OOL POL OOS POP POS OOA POA [M+NH 4 ] OOS + [M+NH 4 ] + MS [OL] + [OS] + MS/MS