Minjia Tan, Ph.D Shanghai Institute of Materia Medica, Chinese Academy of Sciences
|
|
- Barbra Jones
- 6 years ago
- Views:
Transcription
1 Lysine Glutarylation Is a Protein Posttranslational Modification Regulated by SIRT5 Minjia Tan, Ph.D Shanghai Institute of Materia Medica, Chinese Academy of Sciences
2 Lysine: the most frequently modified amino acid PTMs on the lysine residue: Acetylation, ubiquitylation, methylation, propionylation. Acetyl Succinyl Tri-methyl Malonyl Lysine Ubiquitinyl Crotonyl Tan et al, Cell : Zhang and Tan et al Nature Chem Biol : Peng et al, Mol Cell Proteomics M
3 The Ksucc proteome in mammalian cell 2,565 Ksucc sites on 779 proteins. Kim et al, Mol Cell :919-30
4 Proteome-wide E. Coli Ksucc Substrates Biological Process translation oxo acid metabolism cellular ketone metabolism cellular amino acid metabolism nucleotide metabolism gene expression Log 10 Adj P 2487 Ksucc sites on 658 proteins ~16% E. coli proteome CobB is a desuccinylase and deacetylase Gozde, Tan et al. Mol Cell Proteomics. 2013,
5 Lysine glutarylation (Kglu) Da Da + 86 Da
6 Detection of Kglu signals by anti-kglu antibody -250kd -150kd Kglu Ksucc Kmal Kac K -100kd 1x -75kd IB: K glu 5x IB: K glu -50kd -37kd 25x -25kd -20kd Dot spot assay Competed with unmodified BSA tryptic peptides Competed with glutarylated-bsa tryptic peptides -15kd -10kd
7 Identification of Kglu peptides by MS HeLa digestion IP HPLC/MS/MS Kglu (+114 Da) peptides identified by MS from HeLa cells. Peptide Sequence Protein Name Site NFSTVDIQKNGVK DNA mismatch repair protein Msh2 K546 TVDGPSGKLWR Glyceraldehyde- 3- phosphate dehydrogenase K194 VGMGGMEAKVK Delta- 1- pyrroline- 5- carboxylate synthase K311 SDVYYFSPSGKK Methyl- CpG- binding domain protein 2 K185 ADGKISEQSDAK ATP synthase subunit alpha K531 TPVTDPATGAVKEK Very- long- chain specific acyl- CoAdehydrogenase K322 VIQGAGKLPR Zinc finger/btb domain- containing protein 7A K396 NFGTKISAR 6- phosphofructokinase type C K688 GKGGEIQPVSVK 10 kda heat shock protein K56 FASDPIIKGSGTAEVELK Pyruvate kinase K105 MS/MS
8 Validation of Kglu 1. MS/MS analysis 2. HPLC Co-elution 100 NFSTVDIQK glu NGVK Synthetic Synthetic z= y 11 y 10 y 9 y 8 y 7 y 5 y 4 N F S T V D I Q K glu N G V K b 2 b In vivo In Vivo z=2 y y 7 b 2 y 4 y y 11 y y y Mixture Time (min)
9 Validation of Kglu 3. In vivo D4-glutrate labeling Glutarate Glutarate Glutarate Anti-K glu -200kDa -150kDa -100kDa -75kDa -50kDa -37kDa -25kDa -20kDa -15kDa -10kDa Anti-K ac -200kDa -150kDa -100kDa -75kDa -50kDa -37kDa -25kDa -20kDa -15kDa -10kDa Coomassie Blue -200kDa -150kDa -100kDa -75kDa -50kDa -37kDa -25kDa -20kDa -15kDa -10kDa 100 y y 12 y 11 y 10 y 9 y 8 y 7 y 6 y 5 N F S T V D I Q K 4D-Glu y 4 y 3 N G V K b 2 b 3 b 4 b 5 b 6 b 8 b 12 0 b y b * y * * b y b y y b y y y m/z b y *
10 What is the regulatory enzyme?
11 Screening regulatory enzymes for dekglu HC Acyllysine compound based assay HN NH H N HDACs Boc-Lys(glutaryl)-AMC Fluorescence (AU x10 5 ) µg Protein 2 µg Protein NH H N Boc-Lys-AMC 0 SIRT1 SIRT2 SIRT3 SIRT4 SIRT5 SIRT6 SIRT7 H 2 N Trypsin NH H 2 N 4-Amino-methylcoumarin fluorescence,30-folds (Ex=390nm,Em=460nm) H 2 N
12 SIRT5 as a dekglu enzyme in vitro 32 P-NAD + consumption assay Kglu peptide assay NAD + mau : VKSKATNLWW : VKSK glu ATNLWW z=2 613 m/z z=2 670 m/z Sirt5 +Sirt5 +Sirt5- HY Sirt5 - NAD 0 o-glutaryl-adp ribose, G-ADPR; o-succinyl-adp ribose, S-ADPR; o-acetyl-adp ribose, A-ADPR Sirt5 +NAM +Sirt5 +Sirtinol +Sirt5 +TSA +Sirt5 +NaBu min
13 SIRT5 catalyzes dekglu in vivo Pair 1 Pair 2 Pair 3 Pair 1 Pair 2 Pair 3 Pair 1 Pair 2 Pair 3-200kDa -150kDa -100kDa -75kDa -50kDa -37kDa -25kDa -20kDa -15kDa -200kDa -150kDa -100kDa -75kDa -50kDa -37kDa -25kDa -20kDa -15kDa -200kDa -150kDa -100kDa -75kDa -50kDa -37kDa -25kDa -20kDa -15kDa -10kDa -10kDa -10kDa Coomassie Blue Anti-K glu Anti-K succ Mitochondrial lysates from WT and Sirt5 K mouse livers
14 Sirt5 is the regulatory enzyme for Kmal/Ksucc Lysine Peng et al, Mol Cell Proteomics. 2011,,M
15 What are the preferred deacylation susbtrates of SIRT5?
16 Kinetic studies for SIRT5 deacylation activity
17 In silico molecular modeling K glu K mal K succ K adi K ac
18 Lys Acylation and Sirtuins Mann M., et al, Nat Rev Mol Cell Biol Aug;15:
19 Physiological significane of Kglu?
20 Biosynthetic Pathway of Glutaryl-CoA
21 Protein hyperglutarylation is sensitive to dietary changes Coomassie Blue Anti-K ac Anti-K glu The effect of tryptophan on the Kglu levels of Drosophila
22 Protein hyperglutarylation is sensitive to dietary changes Set 1 Set 2 Anti-K glu Lysate from WT and Sirt5 K 48h-fed and fasted mice livers.
23 Proteome-wide survey of Kglu sites in SIRT5K mouse liver 683 Kglu sites in 191 proteins Carbamoyl-phosphate synthetase 1 氨甲酰磷酸合成酶 (CPS1): 33 Kglu sites Key enzyme for ammonia detoxification
24 CPS1 is targeted for dekglu by SIRT5
25 Candidate Kglu sites regulated by SIRT5 Chemical glutarylated CPS1 Kglu site Peptide Sequence SIRT5 SIRT5 In vivo - + K55 AQTAHIVLEDGTKMK Yes No Yes K219 GNPTKVVAVDCGIK Yes No Yes K412 ATTITSVLPKPALVASR Yes No Yes K889 DILNMEKTLK Yes No Yes K892 TLKGLNSESMTEETLK Yes No Yes K915 AKEIGFSDKQISK Yes No Yes K1360 IPQKGILIGIQQSFRPR Yes No Yes K1486 KVDSKSLFHYR Yes No Yes
26 Structural analysis on glutarylated CPS1 homology model allosteric activation K1486 CPS1 oligomerization domain glutamine amidotransferase domain (structurally and interaction)
27 Pathological singnificance of Kglu?
28 Biosynthetic Pathway of Glutaryl-CoA human glutaric acidemia I
29 Protein hyperglutarylation in GCDH deficient mouse A A B K glu C
30 Kglu and Glutaric Acidemia I (GA) Crotonyl-CoA Tan et al, Cell Metabolism, 2014, 19,
31 Acknowledgements Prof. Zhao s Lab Chao Peng Zhonyu Xie Lunzhi Dai Gozde Colak Hao Luo Sangkyu Lee Prof. Matt Hirschey Prof. David Lombard
Biochemistry 2 Recita0on Amino Acid Metabolism
Biochemistry 2 Recita0on Amino Acid Metabolism 04-20- 2015 Glutamine and Glutamate as key entry points for NH 4 + Amino acid catabolism Glutamine synthetase enables toxic NH 4 + to combine with glutamate
More informationubiquitinylation succinylation butyrylation hydroxylation crotonylation
Supplementary Information S1 (table) Overview of histone core-modifications histone, residue/modification H1 H2A methylation acetylation phosphorylation formylation oxidation crotonylation hydroxylation
More informationFunctional interplay between protein acylation and cellular metabolism in metabolic disorders Pougovkina, O.A.
UvA-DARE (Digital Academic Repository) Functional interplay between protein acylation and cellular metabolism in metabolic disorders Pougovkina, O.A. Link to publication Citation for published version
More informationSupporting Information. Lysine Propionylation to Boost Proteome Sequence. Coverage and Enable a Silent SILAC Strategy for
Supporting Information Lysine Propionylation to Boost Proteome Sequence Coverage and Enable a Silent SILAC Strategy for Relative Protein Quantification Christoph U. Schräder 1, Shaun Moore 1,2, Aaron A.
More informationAmino acid Catabolism
Enzymatic digestion of dietary proteins in gastrointestinal-tract. Amino acid Catabolism Amino acids: 1. There are 20 different amino acid, they are monomeric constituents of proteins 2. They act as precursors
More informationProteins are sometimes only produced in one cell type or cell compartment (brain has 15,000 expressed proteins, gut has 2,000).
Lecture 2: Principles of Protein Structure: Amino Acids Why study proteins? Proteins underpin every aspect of biological activity and therefore are targets for drug design and medicinal therapy, and in
More information6. How Are Fatty Acids Produced? 7. How Are Acylglycerols and Compound Lipids Produced? 8. How Is Cholesterol Produced?
Lipid Metabolism Learning bjectives 1 How Are Lipids Involved in the Generationand Storage of Energy? 2 How Are Lipids Catabolized? 3 What Is the Energy Yield from the xidation of Fatty Acids? 4 How Are
More informationBiochemistry: A Short Course
Tymoczko Berg Stryer Biochemistry: A Short Course Second Edition CHAPTER 30 Amino Acid Degradation and the Urea Cycle 2013 W. H. Freeman and Company Chapter 30 Outline Amino acids are obtained from the
More informationMetabolism. Metabolic pathways. BIO 5099: Molecular Biology for Computer Scientists (et al) Lecture 11: Metabolic Pathways
BIO 5099: Molecular Biology for Computer Scientists (et al) Lecture 11: Metabolic Pathways http://compbio.uchsc.edu/hunter/bio5099 Larry.Hunter@uchsc.edu Metabolism Metabolism is the chemical change of
More informationMidterm 2. Low: 14 Mean: 61.3 High: 98. Standard Deviation: 17.7
Midterm 2 Low: 14 Mean: 61.3 High: 98 Standard Deviation: 17.7 Lecture 17 Amino Acid Metabolism Review of Urea Cycle N and S assimilation Last cofactors: THF and SAM Synthesis of few amino acids Dietary
More informationIn glycolysis, glucose is converted to pyruvate. If the pyruvate is reduced to lactate, the pathway does not require O 2 and is called anaerobic
Glycolysis 1 In glycolysis, glucose is converted to pyruvate. If the pyruvate is reduced to lactate, the pathway does not require O 2 and is called anaerobic glycolysis. If this pyruvate is converted instead
More informationMidterm 2 Results. Standard Deviation:
Midterm 2 Results High: Low: Mean: Standard Deviation: 97.5% 16% 58% 16.3 Lecture 17 Amino Acid Metabolism Urea Cycle N and S assimilation Last cofactors: THF and SAM Dietary (Exogenous) Proteins Hydrolyzed
More informationANSC 689 PHYSIOLOGICAL CHEMISTRY OF LIVESTOCK SPECIDS. Enzyme Kinetics and Control Reactions
Handout Enzyme Kinetics and Control Reactions ANSC 689 PHYSIOLOGICAL CHEMISTRY OF LIVESTOCK SPECIDS Enzyme Kinetics and Control Reactions I. Kinetics A. Reaction rates 1. First order (reaction rate is
More informationAmino Acid Oxidation and the Urea Cycle
Amino Acid Oxidation and the Urea Cycle Amino Acids: Final class of biomolecules whose oxidation contributes significantly to the generation of energy Undergo oxidation in three metabolic circumstances
More informationnumber Done by Corrected by Doctor F. Al-Khateeb
number 23 Done by A. Rawajbeh Corrected by Doctor F. Al-Khateeb Ketone bodies Ketone bodies are used by the peripheral tissues like the skeletal and cardiac muscles, where they are the preferred source
More informationChapter 10. Introduction to Nutrition and Metabolism, 3 rd edition David A Bender Taylor & Francis Ltd, London 2002
Chapter 10 Introduction to Nutrition and Metabolism, 3 rd edition David A Bender Taylor & Francis Ltd, London 2002 Chapter 10: Integration and Control of Metabolism Press the space bar or click the mouse
More informationLecture: Amino Acid catabolism: Nitrogen-The Urea cycle
BIOC 423: Introductory Biochemistry Biochemistry Education Department of Biochemistry & Molecular Biology University of New Mexico Lecture: Amino Acid catabolism: Nitrogen-The Urea cycle OBJECTIVES Describe
More informationLecture 11 - Biosynthesis of Amino Acids
Lecture 11 - Biosynthesis of Amino Acids Chem 454: Regulatory Mechanisms in Biochemistry University of Wisconsin-Eau Claire 1 Introduction Biosynthetic pathways for amino acids, nucleotides and lipids
More informationFind this material useful? You can help our team to keep this site up and bring you even more content consider donating via the link on our site.
Find this material useful? You can help our team to keep this site up and bring you even more content consider donating via the link on our site. Still having trouble understanding the material? Check
More informationMetabolism of amino acids. Vladimíra Kvasnicová
Metabolism of amino acids Vladimíra Kvasnicová Classification of proteinogenic AAs -metabolic point of view 1) biosynthesis in a human body nonessential (are synthesized) essential (must be present in
More informationThe Role of Protein Domains in Cell Signaling
The Role of Protein Domains in Cell Signaling Growth Factors RTK Ras Shc PLCPLC-γ P P P P P P GDP Ras GTP PTB CH P Sos Raf SH2 SH2 PI(3)K SH3 SH3 MEK Grb2 MAPK Phosphotyrosine binding domains: PTB and
More informationSkeletal muscle acetylation in response to an acute and chronic high fat diet
Skeletal muscle acetylation in response to an acute and chronic high fat diet John Wesley Kavanaugh Dissertation submitted to the faculty of Virginia Polytechnic Institute and State University in partial
More informationIII. Metabolism The Citric Acid Cycle
Department of Chemistry and Biochemistry University of Lethbridge III. Metabolism The Citric Acid Cycle Slide 1 The Eight Steps of the Citric Acid Cycle Enzymes: 4 dehydrogenases (2 decarboxylation) 3
More informationBio 366: Biological Chemistry II Test #2, 100 points total
Bio 366: Biological Chemistry II Test #2, 100 points total Please neatly PRINT YOUR NAME on EACH PAGE. PRINT the l ast four digits of your SOCIAL SECURITY NUMBER on the BACK SIDE OF PAGE 11 of this test.
More informationAhmad Ulnar. Faisal Nimri ... Dr.Faisal
24 Ahmad Ulnar Faisal Nimri... Dr.Faisal Fatty Acid Synthesis - Occurs mainly in the Liver (to store excess carbohydrates as triacylglycerols(fat)) and in lactating mammary glands (for the production of
More informationMarah Bitar. Faisal Nimri ... Nafeth Abu Tarboosh
8 Marah Bitar Faisal Nimri... Nafeth Abu Tarboosh Summary of the 8 steps of citric acid cycle Step 1. Acetyl CoA joins with a four-carbon molecule, oxaloacetate, releasing the CoA group and forming a six-carbon
More informationRoles of Lipids. principal form of stored energy major constituents of cell membranes vitamins messengers intra and extracellular
Roles of Lipids principal form of stored energy major constituents of cell membranes vitamins messengers intra and extracellular = Oxidation of fatty acids Central energy-yielding pathway in animals. O
More informationIntegration of Metabolism
Integration of Metabolism Metabolism is a continuous process. Thousands of reactions occur simultaneously in order to maintain homeostasis. It ensures a supply of fuel, to tissues at all times, in fed
More information(de novo synthesis of glucose)
Gluconeogenesis (de novo synthesis of glucose) Gluconeogenesis Gluconeogenesis is the biosynthesis of new glucose. The main purpose of gluconeogenesis is to maintain the constant blood Glc concentration.
More informationMultiple choice: Circle the best answer on this exam. There are 12 multiple choice questions, each question is worth 3 points.
CHEM 4420 Exam 4 Spring 2015 Dr. Stone Page 1 of 6 Name Use complete sentences when requested. There are 120 possible points on this exam. Therefore there are 20 bonus points. Multiple choice: Circle the
More informationLecture 16. Finish lipid metabolism (Triglycerides, Isoprenoids/Steroids, Glyoxylate cycle) Amino acid metabolism (Urea cycle) Google Man III
Lecture 16 Finish lipid metabolism (Triglycerides, Isoprenoids/Steroids, Glyoxylate cycle) Amino acid metabolism (Urea cycle) Google Man III The Powertrain of Human Metabolism (verview) CARBHYDRATES PRTEINS
More informationPrevious Class. Today. Detection of enzymatic intermediates: Protein tyrosine phosphatase mechanism. Protein Kinase Catalytic Properties
Previous Class Detection of enzymatic intermediates: Protein tyrosine phosphatase mechanism Today Protein Kinase Catalytic Properties Protein Phosphorylation Phosphorylation: key protein modification
More informationPoints 1. Following is the overall reaction catalyzed by the Calvin-Benson cycle:
BCH 4054 February 22, 2002 HOUR TEST 2 NAME_ Points 1. Following is the overall reaction catalyzed by the Calvin-Benson cycle: CO 2 + 3ATP + 2NADPH 1/3 glyceraldehyde-3-p + 3ADP + 2NADP + Give the structures
More informationPart III => METABOLISM and ENERGY. 3.5 Protein Catabolism 3.5a Protein Degradation 3.5b Amino Acid Breakdown 3.5c Urea Cycle
Part III => METABOLISM and ENERGY 3.5 Protein Catabolism 3.5a Protein Degradation 3.5b Amino Acid Breakdown 3.5c Urea Cycle Section 3.5a: Protein Degradation Synopsis 3.5a - Dietary proteins are degraded
More information4. Which step shows a split of one molecule into two smaller molecules? a. 2. d. 5
1. Which of the following statements about NAD + is false? a. NAD + is reduced to NADH during both glycolysis and the citric acid cycle. b. NAD + has more chemical energy than NADH. c. NAD + is reduced
More informationAerobic Fate of Pyruvate. Chapter 16 Homework Assignment. Chapter 16 The Citric Acid Cycle
Chapter 16 Homework Assignment The following problems will be due once we finish the chapter: 1, 3, 7, 10, 16, 19, 20 Additional Problem: Write out the eight reaction steps of the Citric Acid Cycle, using
More informationWelcome to Class 14! Class 14: Outline and Objectives. Overview of amino acid catabolism! Introductory Biochemistry!
Welcome to Class 14 Introductory Biochemistry Class 14: Outline and Objectives Amino Acid Catabolism Fates of amino groups transamination urea cycle Fates of carbon skeletons important cofactors metabolic
More informationLecture 16. Finish lipid metabolism (Triglycerides, Isoprenoids/Steroids, Glyoxylate cycle) Amino acid metabolism (Urea cycle) Google Man III
Lecture 16 Finish lipid metabolism (Triglycerides, Isoprenoids/Steroids, Glyoxylate cycle) Amino acid metabolism (Urea cycle) Google Man III The Powertrain of Human Metabolism (verview) CARBHYDRATES PRTEINS
More informationPost-translational modifications of proteins in gene regulation under hypoxic conditions
203 Review Article Post-translational modifications of proteins in gene regulation under hypoxic conditions 1, 2) Olga S. Safronova 1) Department of Cellular Physiological Chemistry, Tokyo Medical and
More informationBio 366: Biological Chemistry II Final Exam, 100 points total
Bio 366: Biological Chemistry II Final Exam, 100 points total Please neatly print your name on the top of each page, and put the last four digits of your social security number and the sticker from your
More informationModule No. # 01 Lecture No. # 19 TCA Cycle
Biochemical Engineering Prof. Dr. Rintu Banerjee Department of Agricultural and Food Engineering Asst. Prof. Dr. Saikat Chakraborty Department of Chemical Engineering Indian Institute of Technology, Kharagpur
More informationCHE 242 Exam 3 Practice Questions
CHE 242 Exam 3 Practice Questions Glucose metabolism 1. Below is depicted glucose catabolism. Indicate on the pathways the following: A) which reaction(s) of glycolysis are irreversible B) where energy
More informationCellular Pathways That Harvest Chemical Energy. Cellular Pathways That Harvest Chemical Energy. Cellular Pathways In General
Cellular Pathways That Harvest Chemical Energy A. Obtaining Energy and Electrons from Glucose Lecture Series 12 Cellular Pathways That Harvest Chemical Energy B. An Overview: Releasing Energy from Glucose
More informationKidney. Heart. Lung. Sirt1. Gapdh. Mouse IgG DAPI. Rabbit IgG DAPI
a e Na V 1.5 Ad-LacZ Ad- 110KD b Scn5a/ (relative to Ad-LacZ) f 150 100 50 0 p = 0.65 Ad-LacZ Ad- c Heart Lung Kidney Spleen 110KD d fl/fl c -/- DAPI 20 µm Na v 1.5 250KD fl/fl Rabbit IgG DAPI fl/fl Mouse
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature11700 Figure 1: RIP3 as a potential Sirt2 interacting protein. Transfected Flag-tagged Sirt2 was immunoprecipitated from cells and eluted from the Sepharose beads using Flag peptide.
More informationMBB 115:511 and 694:407 Final Exam Niederman/Deis
MBB 115:511 and 694:407 Final Exam Niederman/Deis Tue. Dec. 19, 2006 Name Row Letter Seat Number This exam consists of two parts. Part I is multiple choice. Each of these 25 questions is worth two points.
More informationPTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System
Application Note LC/MS PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Purpose This application note describes an automated workflow
More informationnumber Done by Corrected by Doctor Nayef Karadsheh
number 13 Done by Asma Karameh Corrected by Saad hayek Doctor Nayef Karadsheh Gluconeogenesis This lecture covers gluconeogenesis with aspects of: 1) Introduction to glucose distribution through tissues.
More informationSupplementary Figure S1. Appearacne of new acetyl groups in acetylated lysines using 2,3-13 C 6 pyruvate as a tracer instead of labeled glucose.
Supplementary Figure S1. Appearacne of new acetyl groups in acetylated lysines using 2,3-13 C 6 pyruvate as a tracer instead of labeled glucose. (a) Relative levels of both new acetylation and all acetylation
More informationCellular Respiration
Cellular Respiration 1. To perform cell work, cells require energy. a. A cell does three main kinds of work: i. Mechanical work, such as the beating of cilia, contraction of muscle cells, and movement
More information7 Pathways That Harvest Chemical Energy
7 Pathways That Harvest Chemical Energy Pathways That Harvest Chemical Energy How Does Glucose Oxidation Release Chemical Energy? What Are the Aerobic Pathways of Glucose Metabolism? How Is Energy Harvested
More informationdoi: /nature10642
doi:10.1038/nature10642 Supplementary Fig. 1. Citric acid cycle (CAC) metabolism in WT 143B and CYTB 143B cells. a, Proliferation of WT 143B and CYTB 143B cells. Doubling times were 28±1 and 33±2 hrs for
More informationNitrogen Metabolism. Overview
Nitrogen Metabolism Pratt and Cornely Chapter 18 Overview Nitrogen assimilation Amino acid biosynthesis Nonessential aa Essential aa Nucleotide biosynthesis Amino Acid Catabolism Urea Cycle Juicy Steak
More informationChapter 9 Overview. Aerobic Metabolism I: The Citric Acid Cycle. Live processes - series of oxidation-reduction reactions. Aerobic metabolism I
n n Chapter 9 Overview Aerobic Metabolism I: The Citric Acid Cycle Live processes - series of oxidation-reduction reactions Ingestion of proteins, carbohydrates, lipids Provide basic building blocks for
More information18 Amino Acid Oxidation and Production of Urea W. H. Freeman and Company
18 Amino Acid Oxidation and Production of Urea 2013 W. H. Freeman and Company 1 Last Class of Biomolecules For Energy 1. Production of acetyl-coa. Glucose. To pyruvate via glycolysis. To acetyl-coa by
More informationBiochemistry 423 Final Examination NAME:
Biochemistry 423 Final Examination NAME: 1 Circle the single BEST answer (3 points each) 1. At equilibrium the free energy of a reaction G A. depends only on the temperature B. is positive C. is 0 D. is
More informationSynthesis of Fatty Acids and Triacylglycerol
Synthesis of Fatty Acids and Triacylglycerol Lippincott s Chapter 16 Fatty Acid Synthesis Mainly in the Liver Requires Carbon Source: Acetyl CoA Reducing Power: NADPH 8 CH 3 COO C 15 H 33 COO Energy Input:
More informationBiochemistry: A Short Course
Tymoczko Berg Stryer Biochemistry: A Short Course Second Edition CHAPTER 30 Amino Acid Degradation and the Urea Cycle 2013 W. H. Freeman and Company In the cytosol of a cell amino groups from amino acids
More informationYield of energy from glucose
Paper : Module : 05 Yield of Energy from Glucose Principal Investigator, Paper Coordinator and Content Writer Prof. Ramesh Kothari, Professor Dept. of Biosciences, Saurashtra University, Rajkot - 360005
More informationNITROGEN METABOLISM An Overview
1 University of Papua New Guinea School of Medicine and Health Sciences Division of Basic Medical Sciences Discipline of Biochemistry and Molecular Biology PBL Seminar & Health Sciences NITROGEN METABOLISM
More informationNitrogen Metabolism. Overview
Nitrogen Metabolism Pratt and Cornely Chapter 18 Overview Nitrogen assimilation Amino acid biosynthesis Nonessential aa Essential aa Nucleotide biosynthesis Amino Acid Catabolism Urea Cycle Juicy Steak
More informationIntegrative Metabolism: Significance
Integrative Metabolism: Significance Energy Containing Nutrients Carbohydrates Fats Proteins Catabolism Energy Depleted End Products H 2 O NH 3 ADP + Pi NAD + NADP + FAD + Pi NADH+H + NADPH+H + FADH2 Cell
More informationPathway overview. Glucose + 2NAD + + 2ADP +2Pi 2NADH + 2pyruvate + 2ATP + 2H 2 O + 4H +
Glycolysis Glycolysis The conversion of glucose to pyruvate to yield 2ATP molecules 10 enzymatic steps Chemical interconversion steps Mechanisms of enzyme conversion and intermediates Energetics of conversions
More informationANSC 619 PHYSIOLOGICAL CHEMISTRY OF LIVESTOCK SPECIES. Carbohydrate Metabolism
ANSC 619 PHYSIOLOGICAL CHEMISTRY OF LIVESTOCK SPECIES I. Glycolysis A. Pathway Regulation of glycolysis Hexokinase: Activated by glucose. Inhibited by G6P. 6-Phosphofructokinase: Inhibited by ATP, especially
More informationPhotosynthesis in chloroplasts. Cellular respiration in mitochondria ATP. ATP powers most cellular work
Light energy ECOSYSTEM CO + H O Photosynthesis in chloroplasts Cellular respiration in mitochondria Organic molecules + O powers most cellular work Heat energy 1 becomes oxidized (loses electron) becomes
More informationChemistry 3503 Final exam April 17, Student s name:
Chemistry 3503 Final exam April 17, 2008 Student s name: THIS EXAM IS FOR STUDENTS IN D. CRAIG S SECTION. IF YOU ARE IN M. EZE S SECTION THIS EXAM IS NOT FOR YOU. Part I /40 Part II Question 1 /4 Question
More informationAmino acid metabolism
Amino acid metabolism The important reaction commonly employed in the breakdown of an amino acid is always the removal of its -amino group. The product ammonia is excreted after conversion to urea or other
More informationCellular Respiration
Cellular I can describe cellular respiration Cellular respiration is a series of metabolic pathways releasing energy from a foodstuff e.g. glucose. This yields energy in the form of ATP adenosine P i P
More informationSecondary Energy Deficiencies in Organic Acidemias. Kimberly A Chapman MD PhD Children s National July 26, 2014
Secondary Energy Deficiencies in Organic Acidemias Kimberly A Chapman MD PhD Children s National July 26, 2014 2 Goals of this talk Describe the secondary energy deficiencies seen in organic acidemias
More informationEnergy storage in cells
Energy storage in cells Josef Fontana EC - 58 Overview of the lecture Introduction to the storage substances of human body Overview of storage compounds in the body Glycogen metabolism Structure of glycogen
More informationDental Students Biochemistry Exam V Questions ( Note: In all cases, the only correct answer is the best answer)
Dental Students Biochemistry Exam V Questions - 2006 ( Note: In all cases, the only correct answer is the best answer) 1. Essential fatty acids are: A. precursors of biotin B. precursors of tyrosine C.
More informationUse of Derivatization for LC- MS/MS Analysis in the Clinical Lab
Use of Derivatization for LC- MS/MS Analysis in the Clinical Lab Asian Pacific Conference on Chromatography & Mass Spectrometry 2010 15 January 2010 Alan L. Rockwood and Mark M. Kushnir ARUP Laboratories,
More informationAmino acids. Side chain. -Carbon atom. Carboxyl group. Amino group
PROTEINS Amino acids Side chain -Carbon atom Amino group Carboxyl group Amino acids Primary structure Amino acid monomers Peptide bond Peptide bond Amino group Carboxyl group Peptide bond N-terminal (
More information2. (12 pts) Given the following metabolic pathway (as it occurs in the cell):
Answer Sheet 1 (Gold) 1. (1 pt) Write your exam ID (A) in the blank at the upper right of your answer sheet. 2. (12 pts) Given the following metabolic pathway (as it occurs in the cell): a. Would you expect
More informationEnzymes Part III: regulation II. Dr. Mamoun Ahram Summer, 2017
Enzymes Part III: regulation II Dr. Mamoun Ahram Summer, 2017 Advantage This is a major mechanism for rapid and transient regulation of enzyme activity. A most common mechanism is enzyme phosphorylation
More informationMetabolic integration and Regulation
Metabolic integration and Regulation 109700: Graduate Biochemistry Trimester 2/2016 Assistant Prof. Dr. Panida Khunkaewla kpanida@sut.ac.th School of Chemistry Suranaree University of Technology 1 Overview
More informationFatty acids synthesis
Fatty acids synthesis The synthesis start from Acetyl COA the first step requires ATP + reducing power NADPH! even though the oxidation and synthesis are different pathways but from chemical part of view
More informationConcept 8.3: ATP powers cellular work by coupling exergonic reactions to endergonic reactions
Concept 8.3: ATP powers cellular work by coupling exergonic reactions to endergonic reactions A cell does three main kinds of work: Chemical Transport Mechanical To do work, cells manage energy resources
More informationNitrogen Metabolism. Pratt and Cornely Chapter 18
Nitrogen Metabolism Pratt and Cornely Chapter 18 Overview Nitrogen assimilation Amino acid biosynthesis Nonessential aa Essential aa Nucleotide biosynthesis Amino Acid Catabolism Urea Cycle Juicy Steak
More informationChapter 9. Cellular Respiration and Fermentation
Chapter 9 Cellular Respiration and Fermentation Energy flows into an ecosystem as sunlight and leaves as heat Photosynthesis generates O 2 and organic molecules, which are used in cellular respiration
More informationANSC/NUTR 618 Lipids & Lipid Metabolism
I. Overall concepts A. Definitions ANC/NUTR 618 Lipids & Lipid Metabolism 1. De novo synthesis = synthesis from non-fatty acid precursors a. Carbohydrate precursors (glucose, lactate, and pyruvate) b.
More informationLecture 19: Review of regulation
Chem*3560 Lecture 19: Review of regulation What is meant by cooperative allosteric regulation? Positive cooperativity - characteristic is the sigmoidal binding/activity curve T-state has weaker affinity,
More informationSynthesis of Fatty Acids and Triacylglycerol
Fatty Acid Synthesis Synthesis of Fatty Acids and Triacylglycerol Requires Carbon Source: Reducing Power: NADPH Energy Input: ATP Why Energy? Why Energy? Fatty Acid Fatty Acid + n(atp) ΔG o : -ve Fatty
More informationAnswer three from questions 5, 6, 7, 8, and 9.
BCH 4053 May 1, 2003 FINAL EXAM NAME There are 9 pages and 9 questions on the exam. nly five are to be answered, each worth 20 points. Answer two from questions 1, 2, 3, and 4 Answer three from questions
More informationAMINO ACIDS NON-ESSENTIAL ESSENTIAL
Edith Frederika Introduction A major component of food is PROTEIN The protein ingested as part of our diet are not the same protein required by the body Only 40 to 50 gr of protein is required by a normal
More informationNITROGEN METABOLISM: An Overview
NITROGEN METABOLISM: An Overview University of PNG School of Medicine and Health Sciences Division of Basic Medical Sciences Discipline of Biochemistry & Molecular Biology VJ Temple 1 How are nitrogen-containing
More informationChem 109 C. Fall Armen Zakarian Office: Chemistry Bldn 2217
Chem 109 C Fall 2014 Armen Zakarian ffice: Chemistry Bldn 2217 o Catabolism of carbohydrates: 10 reactions of glycolysis Chapter 25 C C 2 C 2 D-glucose α-d-glucopyranose aworth projection α-d-glucopyranose
More informationAP Biology Summer Assignment Chapter 3 Quiz
AP Biology Summer Assignment Chapter 3 Quiz 2016-17 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. All of the following are found in a DNA nucleotide
More informationAMINOACID METABOLISM FATE OF AMINOACIDS & UREA CYCLE
AMINOACID METABOLISM FATE OF AMINOACIDS & UREA CYCLE SOURCE & FATE OF AA The aminoacids obtained from DIETARY SOURCE or BODY PROTEIN TURNOVER are utilized for protein biosynthesis and the production of
More informationUnder aerobic conditions, pyruvate enters the mitochondria where it is converted into acetyl CoA.
Under aerobic conditions, pyruvate enters the mitochondria where it is converted into acetyl CoA. Acetyl CoA is the fuel for the citric acid cycle, which processes the two carbon acetyl unit to two molecules
More informationSection 1 Proteins and Proteomics
Section 1 Proteins and Proteomics Learning Objectives At the end of this assignment, you should be able to: 1. Draw the chemical structure of an amino acid and small peptide. 2. Describe the difference
More informationMetabolic Biochemistry / BIBC 102 Midterm Exam / Spring 2005
Metabolic Biochemistry / BIBC 102 Midterm Exam / Spring 2005 I. (20 points) Fill in all of the enzyme catalyzed reactions which convert glycogen to lactate. Draw the correct structure for each intermediate
More informationReading Assignments. A. Energy and Energy Conversions. Lecture Series 9 Cellular Pathways That Harvest Chemical Energy. gasoline) or elevated mass.
Lecture Series 9 Cellular Pathways That Harvest Chemical Energy Reading Assignments Review Chapter 3 Energy, Catalysis, & Biosynthesis Read Chapter 13 How Cells obtain Energy from Food Read Chapter 14
More informationName: Chem 351 Exam 3
Multiple hoice: Pick the BEST answer and write it in the box at the end of the section. 1) The TA (Krebs) ycle depends on oxygen availability, though it does not directly use it. How can you best explain
More informationCHAPTER 16. Glycolysis
CHAPTER 16 Glycolysis Net reaction of Glycolysis Converts: 1 Glucose Hexose stage 2 pyruvate - Two molecules of ATP are produced - Two molecules of NAD + are reduced to NADH Triose stage Glucose + 2 ADP
More informationCitric acid cycle and respiratory chain. Pavla Balínová
Citric acid cycle and respiratory chain Pavla Balínová Mitochondria Structure of mitochondria: Outer membrane Inner membrane (folded) Matrix space (mtdna, ribosomes, enzymes of CAC, β-oxidation of FA,
More informationCatabolism of Carbon skeletons of Amino acids. Amino acid metabolism
Catabolism of Carbon skeletons of Amino acids Amino acid metabolism Carbon skeleton Carbon Skeleton a carbon skeleton is the internal structure of organic molecules. Carbon Arrangements The arrangement
More informationCITRIC ACID CYCLE ERT106 BIOCHEMISTRY SEM /19 BY: MOHAMAD FAHRURRAZI TOMPANG
CITRIC ACID CYCLE ERT106 BIOCHEMISTRY SEM 1 2018/19 BY: MOHAMAD FAHRURRAZI TOMPANG Chapter Outline (19-1) The central role of the citric acid cycle in metabolism (19-2) The overall pathway of the citric
More informationSheet #13. #Citric acid cycle made by zaid al-ghnaneem corrected by amer Al-salamat date 11/8/2016. Here we go.. Record #18
1 Sheet #13 #Citric acid cycle made by zaid al-ghnaneem corrected by amer Al-salamat date 11/8/2016 Here we go.. Record #18 2 Three processes play central role in aerobic metabolism: 1) The citric acid
More information